LOCUS       DAYYCQ010000001       115740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000001 DAYYCQ010000000
VERSION     DAYYCQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 115740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.001"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHAYF_000001"
     CDS             212..604
                     /locus_tag="ACHAYF_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8577474.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHAYF_000002"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHAYF_000002"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8577475.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHAYF_000003"
     CDS             2698..2943
                     /locus_tag="ACHAYF_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577476.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHAYF_000004"
     CDS             3165..4775
                     /locus_tag="ACHAYF_000004"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8577477.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHAYF_000005"
     CDS             complement(4752..4964)
                     /locus_tag="ACHAYF_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577478.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHAYF_000006"
     CDS             5067..5936
                     /locus_tag="ACHAYF_000006"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8577479.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHAYF_000007"
     CDS             6101..7372
                     /locus_tag="ACHAYF_000007"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8577480.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHAYF_000008"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHAYF_000008"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8577481.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHAYF_000009"
     CDS             8763..9026
                     /locus_tag="ACHAYF_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8577482.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHAYF_000010"
     CDS             9471..9920
                     /locus_tag="ACHAYF_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8577483.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHAYF_000011"
     CDS             9977..11395
                     /locus_tag="ACHAYF_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8577484.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHAYF_000012"
     CDS             11395..12255
                     /locus_tag="ACHAYF_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8577485.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHAYF_000013"
     CDS             12429..13202
                     /locus_tag="ACHAYF_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8577486.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHAYF_000014"
     CDS             13349..13714
                     /locus_tag="ACHAYF_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8577487.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHAYF_000015"
     CDS             complement(13862..15070)
                     /locus_tag="ACHAYF_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8577488.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHAYF_000016"
     CDS             complement(15089..15367)
                     /locus_tag="ACHAYF_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577489.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHAYF_000017"
     CDS             15560..16216
                     /locus_tag="ACHAYF_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8577490.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHAYF_000018"
     CDS             complement(16658..17455)
                     /locus_tag="ACHAYF_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8577491.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHAYF_000019"
     CDS             complement(17452..18636)
                     /locus_tag="ACHAYF_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8577492.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHAYF_000020"
     CDS             complement(18661..19908)
                     /locus_tag="ACHAYF_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8577493.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHAYF_000021"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHAYF_000021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8577494.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHAYF_000022"
     CDS             21888..22202
                     /locus_tag="ACHAYF_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8577495.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHAYF_000023"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHAYF_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8577496.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHAYF_000024"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHAYF_000024"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8577497.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHAYF_000025"
     CDS             23546..23875
                     /locus_tag="ACHAYF_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8577498.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHAYF_000026"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHAYF_000026"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8577499.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHAYF_000027"
     CDS             24863..25687
                     /locus_tag="ACHAYF_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8577500.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHAYF_000028"
     CDS             25680..26027
                     /locus_tag="ACHAYF_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8577501.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHAYF_000029"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHAYF_000029"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8577502.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHAYF_000030"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHAYF_000030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8577503.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHAYF_000031"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHAYF_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8577504.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHAYF_000032"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHAYF_000032"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8577505.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHAYF_000033"
     CDS             31684..32451
                     /locus_tag="ACHAYF_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8577506.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHAYF_000034"
     CDS             32441..34483
                     /locus_tag="ACHAYF_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8577507.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACHAYF_000035"
     CDS             34764..34913
                     /locus_tag="ACHAYF_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8577508.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACHAYF_000036"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACHAYF_000036"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8577509.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACHAYF_000037"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACHAYF_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8577510.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACHAYF_000038"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACHAYF_000038"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8577511.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACHAYF_000039"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACHAYF_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8577512.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACHAYF_000040"
     CDS             39330..40412
                     /locus_tag="ACHAYF_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8577513.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACHAYF_000041"
     CDS             40412..41215
                     /locus_tag="ACHAYF_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8577514.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACHAYF_000042"
     CDS             41285..42025
                     /locus_tag="ACHAYF_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8577515.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACHAYF_000043"
     CDS             42036..43040
                     /locus_tag="ACHAYF_000043"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8577516.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="ACHAYF_000044"
     CDS             complement(43085..44929)
                     /locus_tag="ACHAYF_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577517.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45140..45658
                     /locus_tag="ACHAYF_000045"
     CDS             45140..45658
                     /locus_tag="ACHAYF_000045"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8577518.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45786..46469
                     /locus_tag="ACHAYF_000046"
     CDS             45786..46469
                     /locus_tag="ACHAYF_000046"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8577519.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46778..46851
                     /locus_tag="ACHAYF_000047"
     tRNA            46778..46851
                     /locus_tag="ACHAYF_000047"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46812..46814,aa:Arg,seq:tct)
     gene            47010..47369
                     /locus_tag="ACHAYF_000048"
     CDS             47010..47369
                     /locus_tag="ACHAYF_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8577520.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47492..49123
                     /locus_tag="ACHAYF_000049"
     CDS             47492..49123
                     /locus_tag="ACHAYF_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8577521.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49185..49826)
                     /locus_tag="ACHAYF_000050"
     CDS             complement(49185..49826)
                     /locus_tag="ACHAYF_000050"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8577522.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50012..50088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50108..50416
                     /gene="rplU"
                     /locus_tag="ACHAYF_000051"
     CDS             50108..50416
                     /gene="rplU"
                     /locus_tag="ACHAYF_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8577523.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50443..50787
                     /locus_tag="ACHAYF_000052"
     CDS             50443..50787
                     /locus_tag="ACHAYF_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8577524.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50800..51093
                     /gene="rpmA"
                     /locus_tag="ACHAYF_000053"
     CDS             50800..51093
                     /gene="rpmA"
                     /locus_tag="ACHAYF_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8577525.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51527..54334
                     /locus_tag="ACHAYF_000054"
     CDS             51527..54334
                     /locus_tag="ACHAYF_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8577526.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54525..55016
                     /locus_tag="ACHAYF_000055"
     CDS             54525..55016
                     /locus_tag="ACHAYF_000055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8577527.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55119..56108
                     /locus_tag="ACHAYF_000056"
     CDS             55119..56108
                     /locus_tag="ACHAYF_000056"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8577528.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56141..56944
                     /locus_tag="ACHAYF_000057"
     CDS             56141..56944
                     /locus_tag="ACHAYF_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8577529.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56965..57879
                     /locus_tag="ACHAYF_000058"
     CDS             56965..57879
                     /locus_tag="ACHAYF_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8577530.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57962..58336
                     /locus_tag="ACHAYF_000059"
     CDS             57962..58336
                     /locus_tag="ACHAYF_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8577531.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58475..58720
                     /locus_tag="ACHAYF_000060"
     CDS             58475..58720
                     /locus_tag="ACHAYF_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8577532.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58892..59956
                     /locus_tag="ACHAYF_000061"
     CDS             58892..59956
                     /locus_tag="ACHAYF_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8577533.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60202..60627
                     /locus_tag="ACHAYF_000062"
     CDS             60202..60627
                     /locus_tag="ACHAYF_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8577534.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60620..61069
                     /gene="nusB"
                     /locus_tag="ACHAYF_000063"
     CDS             60620..61069
                     /gene="nusB"
                     /locus_tag="ACHAYF_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8577535.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61166..62020
                     /locus_tag="ACHAYF_000064"
     CDS             61166..62020
                     /locus_tag="ACHAYF_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8577536.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62021..63358
                     /gene="xseA"
                     /locus_tag="ACHAYF_000065"
     CDS             62021..63358
                     /gene="xseA"
                     /locus_tag="ACHAYF_000065"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8577537.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63363..63587
                     /locus_tag="ACHAYF_000066"
     CDS             63363..63587
                     /locus_tag="ACHAYF_000066"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8577538.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63589..64479
                     /locus_tag="ACHAYF_000067"
     CDS             63589..64479
                     /locus_tag="ACHAYF_000067"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8577539.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64488..65306
                     /locus_tag="ACHAYF_000068"
     CDS             64488..65306
                     /locus_tag="ACHAYF_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8577540.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65413..65862
                     /locus_tag="ACHAYF_000069"
     CDS             65413..65862
                     /locus_tag="ACHAYF_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8577541.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65880..67562
                     /gene="recN"
                     /locus_tag="ACHAYF_000070"
     CDS             65880..67562
                     /gene="recN"
                     /locus_tag="ACHAYF_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8577542.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67617..67733)
                     /locus_tag="ACHAYF_000071"
     CDS             complement(67617..67733)
                     /locus_tag="ACHAYF_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8577543.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67806..68762)
                     /locus_tag="ACHAYF_000072"
     CDS             complement(67806..68762)
                     /locus_tag="ACHAYF_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8577544.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68933..69301
                     /locus_tag="ACHAYF_000073"
     CDS             68933..69301
                     /locus_tag="ACHAYF_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8577545.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69471..69902
                     /gene="mraZ"
                     /locus_tag="ACHAYF_000074"
     CDS             69471..69902
                     /gene="mraZ"
                     /locus_tag="ACHAYF_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8577546.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69923..70882
                     /gene="rsmH"
                     /locus_tag="ACHAYF_000075"
     CDS             69923..70882
                     /gene="rsmH"
                     /locus_tag="ACHAYF_000075"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8577547.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70887..71288
                     /gene="ftsL"
                     /locus_tag="ACHAYF_000076"
     CDS             70887..71288
                     /gene="ftsL"
                     /locus_tag="ACHAYF_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8577548.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71289..73481
                     /locus_tag="ACHAYF_000077"
     CDS             71289..73481
                     /locus_tag="ACHAYF_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8577549.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73507..74469
                     /gene="mraY"
                     /locus_tag="ACHAYF_000078"
     CDS             73507..74469
                     /gene="mraY"
                     /locus_tag="ACHAYF_000078"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8577550.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74483..75844
                     /gene="murD"
                     /locus_tag="ACHAYF_000079"
     CDS             74483..75844
                     /gene="murD"
                     /locus_tag="ACHAYF_000079"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8577551.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75870..76958
                     /gene="murG"
                     /locus_tag="ACHAYF_000080"
     CDS             75870..76958
                     /gene="murG"
                     /locus_tag="ACHAYF_000080"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8577552.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76973..78193
                     /locus_tag="ACHAYF_000081"
     CDS             76973..78193
                     /locus_tag="ACHAYF_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8577553.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78349..79674
                     /gene="ftsA"
                     /locus_tag="ACHAYF_000082"
     CDS             78349..79674
                     /gene="ftsA"
                     /locus_tag="ACHAYF_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8577554.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79697..80938
                     /gene="ftsZ"
                     /locus_tag="ACHAYF_000083"
     CDS             79697..80938
                     /gene="ftsZ"
                     /locus_tag="ACHAYF_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8577555.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80955..81632
                     /locus_tag="ACHAYF_000084"
     CDS             80955..81632
                     /locus_tag="ACHAYF_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8577556.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81646..82245
                     /locus_tag="ACHAYF_000085"
     CDS             81646..82245
                     /locus_tag="ACHAYF_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8577557.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82326..82547
                     /locus_tag="ACHAYF_000086"
     CDS             82326..82547
                     /locus_tag="ACHAYF_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8577558.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82596..83378
                     /locus_tag="ACHAYF_000087"
     CDS             82596..83378
                     /locus_tag="ACHAYF_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8577559.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83399..84106
                     /locus_tag="ACHAYF_000088"
     CDS             83399..84106
                     /locus_tag="ACHAYF_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8577560.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84368..87166
                     /gene="ileS"
                     /locus_tag="ACHAYF_000089"
     CDS             84368..87166
                     /gene="ileS"
                     /locus_tag="ACHAYF_000089"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8577561.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87215..88741)
                     /gene="zwf"
                     /locus_tag="ACHAYF_000090"
     CDS             complement(87215..88741)
                     /gene="zwf"
                     /locus_tag="ACHAYF_000090"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8577562.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88756..89403)
                     /locus_tag="ACHAYF_000091"
     CDS             complement(88756..89403)
                     /locus_tag="ACHAYF_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8577563.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89587..89916)
                     /locus_tag="ACHAYF_000092"
     CDS             complement(89587..89916)
                     /locus_tag="ACHAYF_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8577564.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90093..90821
                     /gene="sfsA"
                     /locus_tag="ACHAYF_000093"
     CDS             90093..90821
                     /gene="sfsA"
                     /locus_tag="ACHAYF_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8577565.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90837..91850
                     /locus_tag="ACHAYF_000094"
     CDS             90837..91850
                     /locus_tag="ACHAYF_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8577566.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91850..93127
                     /locus_tag="ACHAYF_000095"
     CDS             91850..93127
                     /locus_tag="ACHAYF_000095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8577567.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93190..95889
                     /locus_tag="ACHAYF_000096"
     CDS             93190..95889
                     /locus_tag="ACHAYF_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8577568.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQE
                     LLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIYE
                     ETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETLQ
                     RKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYEI
                     KAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFSI
                     DELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFSK
                     IYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96041..96358
                     /locus_tag="ACHAYF_000097"
     CDS             96041..96358
                     /locus_tag="ACHAYF_000097"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8577569.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96388..96708
                     /locus_tag="ACHAYF_000098"
     CDS             96388..96708
                     /locus_tag="ACHAYF_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8577570.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96816..98276
                     /locus_tag="ACHAYF_000099"
     CDS             96816..98276
                     /locus_tag="ACHAYF_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8577571.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98344..98565
                     /locus_tag="ACHAYF_000100"
     CDS             98344..98565
                     /locus_tag="ACHAYF_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577572.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98596..98778
                     /locus_tag="ACHAYF_000101"
     CDS             98596..98778
                     /locus_tag="ACHAYF_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8577573.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98778..99191
                     /locus_tag="ACHAYF_000102"
     CDS             98778..99191
                     /locus_tag="ACHAYF_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8577574.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99314..100495
                     /locus_tag="ACHAYF_000103"
     CDS             99314..100495
                     /locus_tag="ACHAYF_000103"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8577575.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100566..100730)
                     /locus_tag="ACHAYF_000104"
     CDS             complement(100566..100730)
                     /locus_tag="ACHAYF_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577576.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100884..100957
                     /locus_tag="ACHAYF_000105"
     tRNA            100884..100957
                     /locus_tag="ACHAYF_000105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100918..100920,aa:Arg,seq:cct)
     gene            complement(101026..102165)
                     /locus_tag="ACHAYF_000106"
     CDS             complement(101026..102165)
                     /locus_tag="ACHAYF_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8577577.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            102337..102471
                     /locus_tag="ACHAYF_000107"
     CDS             102337..102471
                     /locus_tag="ACHAYF_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577578.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(102464..103099)
                     /locus_tag="ACHAYF_000108"
     CDS             complement(102464..103099)
                     /locus_tag="ACHAYF_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577579.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(103212..103847)
                     /locus_tag="ACHAYF_000109"
     CDS             complement(103212..103847)
                     /locus_tag="ACHAYF_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8577580.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(103881..104342)
                     /locus_tag="ACHAYF_000110"
     CDS             complement(103881..104342)
                     /locus_tag="ACHAYF_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8577581.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(104472..104903)
                     /locus_tag="ACHAYF_000111"
     CDS             complement(104472..104903)
                     /locus_tag="ACHAYF_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8577582.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(104921..105241)
                     /locus_tag="ACHAYF_000112"
     CDS             complement(104921..105241)
                     /locus_tag="ACHAYF_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8577583.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            105540..106316
                     /locus_tag="ACHAYF_000113"
     CDS             105540..106316
                     /locus_tag="ACHAYF_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8577584.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            106331..106534
                     /locus_tag="ACHAYF_000114"
     CDS             106331..106534
                     /locus_tag="ACHAYF_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8577585.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            106550..106888
                     /locus_tag="ACHAYF_000115"
     CDS             106550..106888
                     /locus_tag="ACHAYF_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8577586.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            106875..107054
                     /locus_tag="ACHAYF_000116"
     CDS             106875..107054
                     /locus_tag="ACHAYF_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577587.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(107097..107567)
                     /locus_tag="ACHAYF_000117"
     CDS             complement(107097..107567)
                     /locus_tag="ACHAYF_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577588.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            107654..108352
                     /locus_tag="ACHAYF_000118"
     CDS             107654..108352
                     /locus_tag="ACHAYF_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8577589.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            108530..108871
                     /locus_tag="ACHAYF_000119"
     CDS             108530..108871
                     /locus_tag="ACHAYF_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577590.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            108864..109535
                     /locus_tag="ACHAYF_000120"
     CDS             108864..109535
                     /locus_tag="ACHAYF_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8577591.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            109541..110233
                     /locus_tag="ACHAYF_000121"
     CDS             109541..110233
                     /locus_tag="ACHAYF_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8577592.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            110230..110979
                     /locus_tag="ACHAYF_000122"
     CDS             110230..110979
                     /locus_tag="ACHAYF_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8577593.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            110991..111260
                     /locus_tag="ACHAYF_000123"
     CDS             110991..111260
                     /locus_tag="ACHAYF_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577594.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            111266..111430
                     /locus_tag="ACHAYF_000124"
     CDS             111266..111430
                     /locus_tag="ACHAYF_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577595.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            111423..111722
                     /locus_tag="ACHAYF_000125"
     CDS             111423..111722
                     /locus_tag="ACHAYF_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577596.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            111722..112024
                     /locus_tag="ACHAYF_000126"
     CDS             111722..112024
                     /locus_tag="ACHAYF_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8577597.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            112064..112429
                     /locus_tag="ACHAYF_000127"
     CDS             112064..112429
                     /locus_tag="ACHAYF_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577598.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            112919..113059
                     /locus_tag="ACHAYF_000128"
     CDS             112919..113059
                     /locus_tag="ACHAYF_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577599.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            113056..113238
                     /locus_tag="ACHAYF_000129"
     CDS             113056..113238
                     /locus_tag="ACHAYF_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577600.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            113251..113445
                     /locus_tag="ACHAYF_000130"
     CDS             113251..113445
                     /locus_tag="ACHAYF_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577601.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(113478..113690)
                     /locus_tag="ACHAYF_000131"
     CDS             complement(113478..113690)
                     /locus_tag="ACHAYF_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577602.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKFYSDYWTQTRENELNLEKLIIKKK"
     gene            113818..114018
                     /locus_tag="ACHAYF_000132"
     CDS             113818..114018
                     /locus_tag="ACHAYF_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577603.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            114015..114287
                     /locus_tag="ACHAYF_000133"
     CDS             114015..114287
                     /locus_tag="ACHAYF_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8577604.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            114288..114473
                     /locus_tag="ACHAYF_000134"
     CDS             114288..114473
                     /locus_tag="ACHAYF_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577605.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            114451..114807
                     /locus_tag="ACHAYF_000135"
     CDS             114451..114807
                     /locus_tag="ACHAYF_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577606.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            114767..115243
                     /locus_tag="ACHAYF_000136"
     CDS             114767..115243
                     /locus_tag="ACHAYF_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8577607.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(115389..>115740)
                     /locus_tag="ACHAYF_000137"
     CDS             complement(115389..>115740)
                     /locus_tag="ACHAYF_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8577608.1"
                     /translation="EQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKIKKMLLGKTIE
                     NYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQFIAADYDADLA
                     DQFINGNNFMPTTN"
BASE COUNT        38129 a        19588 c        24152 g        33871 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaatttat cgttattata tgattaagaa ggattgaata
    45121 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    45181 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    45241 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    45301 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    45361 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    45421 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    45481 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    45541 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    45601 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    45661 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    45721 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    45781 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    45841 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    45901 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    45961 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    46021 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    46081 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    46141 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    46201 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    46261 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    46321 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    46381 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    46441 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    46501 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    46561 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    46621 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    46681 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    46741 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    46801 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    46861 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    46921 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    46981 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    47041 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    47101 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    47161 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    47221 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    47281 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    47341 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    47401 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    47461 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    47521 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    47581 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    47641 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    47701 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    47761 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    47821 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    47881 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    47941 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    48001 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    48061 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    48121 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    48181 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    48241 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    48301 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    48361 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    48421 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    48481 gctctccatg cttgtttttg cgccagtgat gtcacagttt ccaatgccag cacttgcagg
    48541 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    48601 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    48661 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    48721 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    48781 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    48841 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    48901 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    48961 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    49021 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    49081 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    49141 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    49201 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    49261 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    49321 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    49381 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    49441 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    49501 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    49561 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    49621 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    49681 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    49741 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    49801 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    49861 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    49921 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    49981 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    50041 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    50101 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    50161 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    50221 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    50281 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    50341 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    50401 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    50461 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    50521 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    50581 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    50641 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    50701 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    50761 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    50821 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    50881 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    50941 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    51001 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    51061 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    51121 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    51181 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    51241 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    51301 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    51361 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    51421 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    51481 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    51541 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    51601 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    51661 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    51721 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    51781 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    51841 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    51901 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    51961 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    52021 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    52081 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    52141 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    52201 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    52261 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    52321 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    52381 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    52441 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    52501 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    52561 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    52621 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    52681 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    52741 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    52801 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    52861 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    52921 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    52981 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    53041 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    53101 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    53161 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    53221 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    53281 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    53341 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    53401 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    53461 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    53521 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    53581 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    53641 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    53701 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    53761 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    53821 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    53881 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    53941 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    54001 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    54061 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    54121 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    54181 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    54241 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    54301 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    54361 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    54421 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    54481 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    54541 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    54601 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    54661 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    54721 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    54781 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    54841 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    54901 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    54961 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    55021 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    55081 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    55141 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    55201 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    55261 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    55321 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    55381 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    55441 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    55501 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    55561 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    55621 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    55681 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    55741 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    55801 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    55861 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    55921 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    55981 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    56041 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    56101 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    56161 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    56221 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    56281 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    56341 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    56401 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    56461 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    56521 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    56581 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    56641 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    56701 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    56761 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    56821 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    56881 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    56941 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    57001 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    57061 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    57121 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    57181 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    57241 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    57301 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    57361 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    57421 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    57481 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    57541 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    57601 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    57661 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    57721 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    57781 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    57841 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    57901 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    57961 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    58021 tctgacagat tacgggcaaa tcatgatagg taataatggt tttgagtttt accatgcgcg
    58081 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    58141 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    58201 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    58261 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    58321 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    58381 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    58441 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    58501 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    58561 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    58621 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    58681 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    58741 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    58801 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    58861 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    58921 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    58981 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    59041 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    59101 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    59161 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    59221 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    59281 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    59341 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    59401 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    59461 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    59521 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    59581 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    59641 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    59701 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    59761 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    59821 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    59881 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    59941 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    60001 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    60061 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    60121 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    60181 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    60241 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    60301 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    60361 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    60421 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    60481 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    60541 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    60601 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    60661 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    60721 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    60781 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    60841 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    60901 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    60961 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    61021 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    61081 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    61141 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    61201 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    61261 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    61321 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    61381 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    61441 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    61501 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    61561 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    61621 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    61681 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    61741 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    61801 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    61861 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    61921 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    61981 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    62041 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    62101 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    62161 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    62221 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    62281 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    62341 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    62401 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    62461 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    62521 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    62581 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    62641 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    62701 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    62761 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    62821 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    62881 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    62941 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    63001 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    63061 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    63121 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    63181 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    63241 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    63301 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    63361 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    63421 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    63481 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    63541 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    63601 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    63661 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    63721 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    63781 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    63841 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    63901 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    63961 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    64021 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    64081 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    64141 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    64201 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    64261 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    64321 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    64381 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    64441 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    64501 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    64561 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    64621 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    64681 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    64741 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    64801 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    64861 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    64921 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    64981 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    65041 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    65101 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    65161 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    65221 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    65281 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    65341 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    65401 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    65461 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    65521 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    65581 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    65641 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    65701 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    65761 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    65821 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    65881 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    65941 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    66001 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    66061 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    66121 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    66181 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    66241 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    66301 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    66361 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    66421 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    66481 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    66541 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    66601 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    66661 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    66721 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    66781 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    66841 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    66901 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    66961 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    67021 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    67081 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    67141 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    67201 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    67261 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    67321 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    67381 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    67441 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    67501 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    67561 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    67621 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    67681 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    67741 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    67801 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    67861 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    67921 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    67981 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    68041 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    68101 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    68161 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    68221 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    68281 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    68341 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    68401 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    68461 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    68521 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    68581 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    68641 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    68701 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    68761 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    68821 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    68881 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    68941 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    69001 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    69061 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    69121 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    69181 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    69241 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    69301 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    69361 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    69421 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    69481 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    69541 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    69601 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    69661 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    69721 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    69781 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    69841 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    69901 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    69961 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    70021 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    70081 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    70141 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    70201 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    70261 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    70321 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    70381 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    70441 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    70501 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    70561 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    70621 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    70681 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    70741 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    70801 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    70861 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    70921 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    70981 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    71041 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    71101 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    71161 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    71221 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    71281 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    71341 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    71401 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    71461 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    71521 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    71581 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    71641 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    71701 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    71761 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    71821 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    71881 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    71941 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    72001 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    72061 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    72121 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    72181 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    72241 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    72301 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    72361 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    72421 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    72481 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    72541 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    72601 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    72661 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    72721 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    72781 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    72841 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    72901 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    72961 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    73021 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    73081 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    73141 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    73201 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    73261 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    73321 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    73381 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    73441 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    73501 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    73561 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    73621 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    73681 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    73741 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    73801 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    73861 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    73921 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    73981 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    74041 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    74101 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    74161 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    74221 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    74281 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    74341 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    74401 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    74461 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    74521 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    74581 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    74641 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    74701 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    74761 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    74821 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    74881 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    74941 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    75001 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    75061 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    75121 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    75181 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    75241 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    75301 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    75361 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    75421 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    75481 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    75541 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    75601 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    75661 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    75721 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    75781 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    75841 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    75901 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    75961 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    76021 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    76081 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    76141 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    76201 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    76261 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    76321 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    76381 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    76441 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    76501 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    76561 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    76621 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    76681 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    76741 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    76801 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    76861 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    76921 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    76981 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    77041 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    77101 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    77161 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    77221 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    77281 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    77341 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    77401 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    77461 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    77521 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    77581 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    77641 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    77701 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    77761 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    77821 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    77881 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    77941 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    78001 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    78061 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    78121 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    78181 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    78241 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    78301 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    78361 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    78421 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    78481 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    78541 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    78601 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    78661 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    78721 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    78781 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    78841 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    78901 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    78961 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    79021 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    79081 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    79141 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    79201 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    79261 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    79321 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    79381 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    79441 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    79501 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    79561 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    79621 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    79681 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    79741 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    79801 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    79861 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    79921 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    79981 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    80041 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    80101 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    80161 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    80221 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    80281 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    80341 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    80401 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    80461 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    80521 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    80581 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    80641 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    80701 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    80761 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    80821 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    80881 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    80941 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    81001 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    81061 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    81121 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    81181 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    81241 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    81301 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    81361 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    81421 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    81481 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    81541 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    81601 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    81661 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    81721 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    81781 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    81841 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    81901 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    81961 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    82021 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    82081 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    82141 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    82201 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    82261 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    82321 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    82381 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    82441 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    82501 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    82561 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    82621 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    82681 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    82741 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    82801 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    82861 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    82921 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    82981 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    83041 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    83101 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    83161 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    83221 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    83281 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    83341 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    83401 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    83461 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    83521 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    83581 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    83641 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    83701 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    83761 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    83821 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    83881 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    83941 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    84001 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    84061 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    84121 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    84181 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    84241 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    84301 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    84361 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    84421 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    84481 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    84541 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    84601 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    84661 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    84721 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    84781 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    84841 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    84901 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    84961 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    85021 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    85081 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    85141 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    85201 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    85261 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    85321 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    85381 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    85441 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    85501 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    85561 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    85621 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    85681 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    85741 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    85801 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    85861 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    85921 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    85981 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    86041 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    86101 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    86161 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    86221 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    86281 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    86341 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    86401 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    86461 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    86521 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    86581 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    86641 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    86701 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    86761 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    86821 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    86881 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    86941 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    87001 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    87061 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    87121 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    87181 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    87241 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    87301 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    87361 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    87421 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    87481 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    87541 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    87601 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    87661 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    87721 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    87781 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    87841 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    87901 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    87961 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    88021 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    88081 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    88141 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    88201 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    88261 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    88321 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    88381 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    88441 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    88501 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    88561 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    88621 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    88681 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    88741 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    88801 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    88861 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    88921 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    88981 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    89041 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    89101 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    89161 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    89221 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    89281 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    89341 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    89401 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    89461 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    89521 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    89581 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    89641 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    89701 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    89761 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    89821 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    89881 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    89941 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    90001 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    90061 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    90121 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    90181 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    90241 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    90301 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    90361 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    90421 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    90481 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    90541 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    90601 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    90661 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    90721 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    90781 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    90841 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    90901 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    90961 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    91021 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    91081 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    91141 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    91201 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    91261 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    91321 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    91381 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    91441 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    91501 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    91561 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    91621 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    91681 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    91741 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    91801 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    91861 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    91921 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    91981 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    92041 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    92101 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    92161 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    92221 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    92281 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    92341 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    92401 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    92461 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    92521 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    92581 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    92641 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    92701 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    92761 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    92821 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    92881 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    92941 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    93001 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    93061 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    93121 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    93181 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    93241 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    93301 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    93361 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    93421 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    93481 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    93541 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    93601 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
    93661 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    93721 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    93781 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    93841 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    93901 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    93961 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    94021 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    94081 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    94141 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    94201 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    94261 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    94321 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    94381 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    94441 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    94501 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    94561 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    94621 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    94681 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga acaagttgag
    94741 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    94801 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    94861 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    94921 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    94981 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    95041 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    95101 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    95161 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    95221 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    95281 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    95341 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    95401 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    95461 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    95521 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    95581 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    95641 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    95701 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    95761 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    95821 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    95881 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    95941 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    96001 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    96061 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    96121 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    96181 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    96241 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    96301 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    96361 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    96421 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    96481 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    96541 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    96601 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    96661 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    96721 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    96781 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    96841 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    96901 gtgatgcttt tatcggcata ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    96961 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    97021 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    97081 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    97141 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    97201 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    97261 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    97321 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    97381 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    97441 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    97501 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    97561 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    97621 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    97681 atggatcaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    97741 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    97801 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    97861 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    97921 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    97981 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    98041 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    98101 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    98161 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    98221 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    98281 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    98341 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    98401 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    98461 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    98521 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    98581 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    98641 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    98701 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    98761 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    98821 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    98881 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    98941 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    99001 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    99061 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    99121 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    99181 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    99241 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    99301 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    99361 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    99421 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    99481 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    99541 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    99601 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    99661 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    99721 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    99781 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    99841 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    99901 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    99961 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
   100021 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
   100081 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
   100141 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
   100201 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
   100261 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
   100321 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
   100381 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
   100441 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
   100501 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
   100561 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
   100621 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
   100681 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
   100741 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
   100801 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
   100861 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
   100921 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
   100981 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
   101041 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
   101101 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
   101161 gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg
   101221 caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac
   101281 ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt
   101341 gatttcaaaa ttctcgaata attctttttg attgatttta aactttttta agagatcaat
   101401 cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt ttccccattc
   101461 atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc
   101521 ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtggcaccag caactaacac
   101581 gagcatagga gaagagtact gcgcagaaag gttttcttct acgagtttca ttaaagattt
   101641 aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat
   101701 tactgctttt ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga
   101761 cgctctaata tggttattaa acttaataac ggtagatttt acatggtctt tagcatactt
   101821 atttaaaaag cgttgatatc ccactctatc taaatcagaa atcaataccg ctggcatata
   101881 tttctttatg ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc
   101941 ttctttataa agttgcatcc aatcctcaaa gtaatctgat aataatagat ttttgcgatc
   102001 ccctttaagg cctttgttca agttatattc taattcgttg gcggcatctt ttgcttctcc
   102061 ttttgtctta aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga
   102121 aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg aatcagctct
   102181 ctttctctga tacaatagga actataaaga agcccattgt ttgggtttta ttattttttg
   102241 cacatccttt ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat ggtataattg
   102301 gatgtgtatt agatcatcca atcagaggtg tttattatga ttaaagtgat ttttaaaaga
   102361 ataactgaac ttccagagtt tagattattt gttgattatt ggtatgtctg gttttttatt
   102421 gttgcattgc tgataggtct agatgtcata ttagaaagaa aacttaagta agagttgagt
   102481 taataaattg aattcctaaa tgagatattt tgtagcccat attttgtttg ccgacatcac
   102541 ctaatactat ggagctaatc gttttttcta acagaccaaa atcagataat ctatttaagc
   102601 taccatctat tagctcttgt gaatagccag acatgctgat atttccagtg aaattttcgt
   102661 ttccacgact cattaagcat gctaacaatt gaatctcaat cgatgttaat tgattcagta
   102721 caagaagata atactcctcg ttatcccatg aagcgttgtt aattcgaagt aaacaattct
   102781 tataaagatt tttatacata tctattttat tttgtgcctt tgatttttct acctcgtcat
   102841 ttatcccttc gaatattcca atgagttggt ccttgttctc taagttgaaa acatcttctg
   102901 gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat ttctcgatgc
   102961 gtttgaaacg cttctcgttt ttagacccaa aataaagtga ctgcacaacg ccaccaatat
   103021 aaggaattgc tccaataccg ccttcaacta ttaattcaag atattctttt ttggttagtt
   103081 tttcattgct attatccata gttacctcct attttgtcat ttcctttaca agcattgtga
   103141 ctcctgatgg cactcttttg tataggtttt attattgtgc acaatctaac tcttagtggg
   103201 gcgtggttca tttatttttt acttacttta ctattaatca tatcccattg agcataggat
   103261 ttttctatgt tcgatcctct tgaactaatt tctcctgtaa aagttacttt atcgcccttt
   103321 gtaaaggtat cagcattgtt taagtccttc gcaaaaatta catatgaaac tttaggtgtt
   103381 gcagaaatat cattccaact tttatcatcg tactctttgt ctgctataat ggctattcta
   103441 gtagtcattg attcgataac agttccttca aaagtatatg ttttaccact aatatattta
   103501 gtaaaagctg tattttgctc agtagctgga gtaatagaat aataactatc taagaaagca
   103561 ccccagtcat gattatttgt ctctaaaaat tcttcaaatt gagttaggct tttattgctg
   103621 ctgttagatg tagtagtttg agtatcagat gatgagcttt cctgtttgtt attgtcattt
   103681 gcctcgtttg atgaggaaag cgagaaacct acggtagtaa aaataatccc gataataagg
   103741 agaatcatag gtgttttctt atttgttttt ttgataaatc ttacaattaa catgattaaa
   103801 cctataataa ataaaacaat accagtaaaa ccaaatatac cgtccattaa tagatcctct
   103861 ttctcttttg atattgaata ttataaacca agcaattctt ttttcttaaa atcgaattct
   103921 tcttgattaa taatcccttc atctaacaac tctttatact ttttaatttc atctgcactt
   103981 gaaatttgtg tttctttgga attattatct aatatagatt gttgcttctc tatatatttt
   104041 tttaaatttt ccatttcatt ccaatatttt tttgtaaaca taacggtgtt ttcatctttc
   104101 gtggccgcta atatacctcc gcgactttcg tttccgccta gcaaggtaaa ttgtatataa
   104161 ccattactca tacctggttt ttttaattgt acggctgata tatttttaaa ggggatagtt
   104221 ttttctcctt taataccttg attaactaaa tttataaatc cttttctaat aattgaaata
   104281 gaatcatcag ttactttaat caaggttttt cctggagatt ttattaaaat ctctttttcc
   104341 attttttctt cctcatttct gtgatatgat tttttttgta gaagctcata tatgaggaaa
   104401 gagcccgtgt tgcagcacgg gcttttttaa tgttttgctg aaatcggttt ttttaataat
   104461 tcttgatgta ttcatacata ttgccttgcg taagaatatt ttttcttaaa atagcattgg
   104521 cagacagcat aacaagtgta tctgagctta ttctgaatag aataatactc cataaatttt
   104581 tcgagattaa actgagattc atcagtcagt tcgttctcaa tatagatatt taataaaatt
   104641 agaatagcta ttttatctgc ttctgtttca aattttgagt gaaaagttgt agaagtatcg
   104701 tacaaaactg aaaattcaaa atgagaggcg ctgaaatgtg caagttcatg agataaatga
   104761 aaggcttctg cagtttcacc gtatagattt tcattcaaaa aaatgattct gggttttgga
   104821 tagtagaaac caggctcttt catctccata taaactactt tcaaattgta ttcgctcaac
   104881 atttctttca atttcaaata catacaaacc atcactccaa ctattcattt tcctctaaag
   104941 ctttagcaat tgcaatcgct ttacgcattg tctccttaga tatttctttt ccatcaaaag
   105001 aaaaaacagt atcgtcttct gataaatcca catgtttagg ggtttctctt tcttctctac
   105061 ctagaaggta gtctacagag acatcgaaat agtcagcaat ccgacttaat tcatctgaat
   105121 taggagtatt atttttccac tttgctagat aaccgttaga atatccaaaa ttaatttcta
   105181 actgccgtat agatatcttt ttcctcttcg ccaagtcttt tattatttca taggtattca
   105241 tcgataaaac aacctttcta aatgtttaca aaaaaagttt agaaaaatac gcagaaataa
   105301 tttgactaat tctgagtaat gagctatact atatcttgta aacagattta acaactaaaa
   105361 agacaacaaa aaacactatt gatatgtaaa tgcagaccgc caagaaagct ttaaaatcaa
   105421 tgtttttatg tcttatttaa ttatgctctt agtatagagt attactcaga tactgtcaat
   105481 tgaatttaga aaaaagttgt taaatttgtt tacgaatata aaagaaagga atgagaaata
   105541 tgaacacacc gcaaattttt aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg
   105601 atgaaccata ttttgtaggc aaagacgttg cgagtgtttt gggttattca aacactaaag
   105661 atgctttgtc gcggcatgta gatttggaag ataagatggg gtcgcgaatc acgacctcag
   105721 gtcaatcaag agagatgaca atcatcaacg aatctggttt gtacagttta atcttaaaat
   105781 caaaacttcc ctctgccaaa aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa
   105841 ttagaaaaca tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag
   105901 atacaatcat tcaattagca actcaactaa aagaagaaag aactggaaga ttaatcgcag
   105961 aacaaaagat tgcagagtac gaacctaaaa tctcctattt agatagcata ttatcttcta
   106021 cagattcagt aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga
   106081 ataaattact tcataaacta ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca
   106141 aaaaacacat gaaccaagga tacacaaaat ctcatacaac tgagatcccg aaagccgatg
   106201 gtggcactaa aattgtaatg aataccaaat ggacacagaa agggcgtcta tttatctacg
   106261 aattactaaa aaaagaagga tattaccctc aaatggattt agaggaaatt ggttagaaag
   106321 gagttttagt atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga
   106381 atatgtcgaa agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag
   106441 caaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat
   106501 tatagattcg attatcacta tgtacgaatt gtaaagggag gaacagctaa tgcaatatct
   106561 agaagcaaaa atcccaattc cagaaggcta tgtaattatc tcccaagtgg attatgagga
   106621 gttaaaaaaa gctgatgata ctggtagatg gatgacgttg ccagaagtgc tagaacggat
   106681 taacagaaaa tatgattggt ttacttctag agttttaaag aacccaagat atagaaatat
   106741 tatcgatatc gagaaaaaca aaaatggatt tgtctattat ccagttgaag gaagagacac
   106801 atatctattt ttaagaagta aaacacttga atttttagaa acaaattttt cggaaatctt
   106861 aaggaggcaa gcggatggga aaatttaata gagcgctagt gttcagcgca ccgctaatca
   106921 tctacgcttt aggactttgg ggaagcaggc aagcgttgat aggaacgatt gtttacatgg
   106981 tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg
   107041 agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag
   107101 tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg
   107161 tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta
   107221 aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata
   107281 tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt
   107341 aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa
   107401 ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaatcata
   107461 tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat tccttttatt
   107521 tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca ttgaaaaatt
   107581 gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta taccaaaaag
   107641 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag
   107701 cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc
   107761 actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc
   107821 atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat
   107881 ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga
   107941 aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta
   108001 tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg
   108061 aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt
   108121 tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt
   108181 ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag
   108241 attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga
   108301 aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag
   108361 cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc
   108421 taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc
   108481 ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga
   108541 actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga
   108601 aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca
   108661 agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa
   108721 gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga
   108781 agagtacgac agagataact tgattgatat ctcttattac ggaccaggta agaaatacat
   108841 tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac
   108901 tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt
   108961 gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg
   109021 agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata
   109081 aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac
   109141 acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa
   109201 atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg
   109261 gactgggatt atatgtattt caaggggaag atattccaac accacctaga atcgatacaa
   109321 agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata
   109381 tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg
   109441 ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa
   109501 tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga
   109561 ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga
   109621 ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagtcat
   109681 tgaaattcgt tttattgatc ctagacgttt caccgtacag caacgaaact tcatctatgc
   109741 gctgataggc gatattttta tcgatacagg catgccaacg gacttctgga aggaattctt
   109801 ctactttcgt tttgaaggtg tcacagggcg caaaataagc ctgaaagacg aatcgaatac
   109861 aactgtgagt gatgccaatg tcttagcaaa tatcatctta gatttcatct ttgaacatca
   109921 tattcctttc aaagaaggct atgagatttt acctgcgaat caagaatatt acttctacaa
   109981 atgcatcaca aaaagagtct gctgcatctg tggcaaaaca ggagctgaca tcgatcactt
   110041 tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat catgcagagt acacatttgc
   110101 agcactctgc agaatccatc acacagagaa gcacaaaata ggtgtgatta atttcaaaaa
   110161 taaatatcaa atcaaaggga tcaaattaaa ccagaaaaca atcaaaaagt taaatattgg
   110221 aggggaaaaa tgacagaaca tcgaagttat tatgcgatta taccagccaa cgtaaggtac
   110281 gacaaaagac ttaaaccaaa tactaagttg ttatacggag agataacggc cttgtgtaat
   110341 gaaagaggct tttgttgggc aggcaatgag tactttgcag atttatatgg tgtgaataaa
   110401 gagaccatat cgcgatgggt aagtgatttg attaagtttg gatacttgaa tcgggaaatc
   110461 atttacaaag agggtaccaa tcaaataatc aataggtacc tacgaattaa tcaataccct
   110521 attgacgaaa aacgcaatac ccctattgac gaaaaagtca aagataataa tacatctatt
   110581 aataatacat ttaataatac aaaagaatat ataagagagt taccgccttc gaaaaaatcg
   110641 aaggctaagc ccgtccgtca taaatacgga gagtataaaa atgttctttt gtcagatgag
   110701 cagatggaga aactcaaaac agaattccct aatgattacc aagagcgaat cgaacgactg
   110761 tcagagtatt gtgaatcatc tggtaagact tataaaaact atttggcaac tattcgaagt
   110821 tgggcaagga aagaaaaaag tgaacctaag aacgcaagca gtggatacaa gcgcacagga
   110881 agacgagaga agcttcctga atgggcaatc gaccaagaag cctatctcaa gaaaaaagcg
   110941 ctagaacgag ctaatagaca atcaaaagca ccattctaag aggtggaaaa atgaagatcg
   111001 attatctaga actaattaat gaaatagcga attataaaaa gggcgaggaa ttagacgtcc
   111061 tgagagacgt atatgatcaa ctcgaagaag ctggaattga aggaattaag aatgatcgtt
   111121 cgagttggag taaactcaga tactatttcg cactctatat cgatggaacg caattaagaa
   111181 atttagcata taccaaatta ctattcattg attgtgttaa aggcttgcaa aaacatctta
   111241 atgaacttga gcaggtgtaa acaagatgga cctaaagaca tttacagcac agatcgaatt
   111301 aatgcatcaa gaagctttaa gacaaagtgt gtcgtacgaa gacaagtggc tcaacacatt
   111361 tcatggcgga cgtgagagcg cacttgatca agtactcaaa ttactgaaag gagaatgtcg
   111421 ggatggataa gaaagcggca atgcagcgaa ttatcgaatt gacttattca gaagattggc
   111481 aggaagataa aaaaatagtt gcagaagtcc aaaagctcgg taaatcaatg tggactgaaa
   111541 agcccaaacg gagaacgccg agaaagattg caatctggca tggtgatcga attctagtaa
   111601 cgggtaccgc taaacagtta tctgaaatta ctggtctgag caaaaatatt atttgggata
   111661 gagctaggag cttatggatt gattcaaaag gaagacagtt taggtatgtg gaggagaaat
   111721 aatggacgaa ctaatcgcga aagtagagca gtgggcaaaa gataagggat tggatcaagc
   111781 ggatttcagc aagcaaatgt taaaaacggt cgaagaaatc ggggaagttg ccgcttctct
   111841 agctagaaaa gatgaacatg gtttaagaga cggaattggc gatgtagtag taaccttgat
   111901 catcttggca atgcaaaatg atatggattt atacgagtgc ttggattttg cttatgacga
   111961 aatcaaaggt cggactggaa agatggtcaa tggtgttttc gttaagtcca gtgacctgaa
   112021 ataattccat taatggaaga aataactgaa taggaggata gacatgattg atgcaaaaca
   112081 agtgcaaaaa cagaaagatg gaatgttgat gtttgaagca tatgttctac catttcttaa
   112141 tcaatttgaa gttttggaat gctctgccag cggagaagaa ttggagtatg ttgtaattcg
   112201 tgaaaccaaa gaaaacgttc agaagctaaa cgaatttctt tgcactatta actgttggga
   112261 tatgatagct ccaggatttt tatgtccagc tatgggagaa tttttggaat attgtcgtct
   112321 tgaagatgca ggcactttgg atttagctta tctggtatac aactacctaa acatcaacac
   112381 agatcatttg tggtttggca ctgccgaaag aaaatgggta gtccgctaac caccaatatc
   112441 aatgaaaaaa ggcggtttcc tgaaagtgga attgccgcca ttgctgtaag gttctttacg
   112501 cgactcaagg tcacagaaat ataaatgcaa atctcttttg aagtgatcca aagctctcgg
   112561 aaaatggaag ttttttgttt caaagaataa atgagatttg cgtcaagaac ctgagagtta
   112621 tttccaaaaa agcttccaaa gaaccgaatg agctaaggac atcatgtgta aacacataat
   112681 atagctctcc tttcacggct aatcataaca tgaaaaatat tcgatgtcag taggaaaaaa
   112741 ggtgaattat gaaaagaata ttaaattatc ccggcagtaa cagattctgc aattaaactc
   112801 cgaagctgat gtatcagtat caactgtaga atattatgag cgtggagaaa gtcaagcagt
   112861 cacaaatgta aaaatcgata acgtcaacgg tcttatctgt ttagatgaag gcgatagtat
   112921 gaatgaaatt gctgcagacc atgaattatc agaaactgct ttaattgatc aaatcgaatt
   112981 agctagacaa tactttgttc catatgcaga tacctggtcc aagcataaga atgacatcat
   113041 ttttccagag gaacaatgag agcaactgat ccagtaatta tccttgagga agccaaattt
   113101 atttggactc acgaagagat agagcaagca cgcttgctct tttctcaagg agttaagccg
   113161 agcaaagtag ctgaaataat gggtcaaaag attcttgatg tcggattgct tttgctccat
   113221 ctagcagaaa aaatttgatt tgaggtgaaa atgatgattc aacttgcagg catccaaaca
   113281 gggaaaattt atttttctgg agaaagcaaa agcgaagcca accaatggct attgaaaact
   113341 tacacgaaca ataagaagct aaggaaaaaa tatcctgatt tgttgctaaa ggatgatcag
   113401 attatgccgg aaccgatgat tttgacccgt aaggagggaa gttgaaacta aaaaaccagc
   113461 cgtgtgggct ggagttgcta tttcttttta ataatgagtt tttctaaatt taattcattc
   113521 tcacgagttt gcgtccaata atcagaataa aattttaatt gtttaagagt aggaatgtcg
   113581 tcttcggtaa aattagaaaa taattctgga gaattatctt tttttaattg ttctaatcct
   113641 gattctttaa aacgttgttc aacgagtttt actaaattgt tgatattcat gataaatcac
   113701 ctttttaaat ttaatttcga tttgattaat cgatacaagt attatatcaa ataaaaaagc
   113761 tgatttaggg ggtcttacaa aaacactaaa attttttcaa aaagaggagg aacggtggtg
   113821 aaagtcagag aacaattcaa gatagtgagc gacgaaggta atgtttacaa aataaagatc
   113881 gttaatatta atgaatatcg agaagcagat cacaaatatt tggttgactt atacatgaac
   113941 gataaatatt tgggagaagg attttgtgga gattcacttt ttgatgatga gcgaattttg
   114001 aaggaggaaa cagaatgatc cacgaactaa aaatactacc agaatatttt gaagcagtga
   114061 cgagcggacg caaacaattc gagatccgca agaatgatcg tgacttcaaa gttggtgatc
   114121 agttaatact acgtgaatat aaaaaataca ttaaacatga attacctgga ctaacgagac
   114181 caacatttag ttacactggt gactcataca aagcagagat cacttatatt actgattaca
   114241 tgcagaagtc cggatatgta gtgttgggga ttaaaggggt gaattaaatg aaagtataca
   114301 tcttagctat cacagagggc acatggatgt tcccagtcgg ttccggaaaa atctataaat
   114361 caaaaacagc tgcttataaa gcatttgaaa aatacaaaaa agaaaacggt ggcggcacta
   114421 acgcaaagat tctagtggct gataactggc atgaagaagg tgaacgtaat tgatttacct
   114481 gttcattttc gtggtactaa ctatttttat gctagtaatc ttattcaaaa aaagatgagt
   114541 aaagacgaac gaaaaattct ggccaacttt tatttagtat taaaaatcga acaaaatgtg
   114601 ttttcggaaa ggagaattgt atgggaaaaa caaaatcaaa gattaagaag aaaaaacgac
   114661 gtttggaaca aaaggcaatc cagaatggaa cggctaagaa aaaataaaaa agctacccct
   114721 ttcgagatag cagcgcaata ctattctacc ataaggggtg gcgtttgtga gatttcattg
   114781 gttaaaagat taccaagaac tagatgagca gattctttac ttaaagtgga atcttaataa
   114841 aagtaagctt gaattgaatc gatgggtctg tggtgattta gcaaacgtcc gcatcgaaaa
   114901 gaattcgaga acttcttctt tagaagagaa tatccaaaag atagaaaatg aattggaatt
   114961 gttgatcgag caaaaagaag aaatgttatt gctgatagat agcttttctg gtatcgataa
   115021 tcaaattgtt aagatgaaat atgttgatca aatgagctta gaagatattg ctgaatctgt
   115081 tgggtatagc tcatcgtatg tcagacagcg acatgcagaa attagaaaga cattgaactt
   115141 tttagatgag tatgaacaca gacaagctga ccgattgaaa aaagaaaacg aaattgattt
   115201 ttacaatagc gagaagtaca aagaacaact ctctttattc taaaattaca ctgttcacta
   115261 aatgttcggt cattgtagct atgtaaacat tgttagcagc atgatattct attagtgtca
   115321 aaaaaatatg aaagagccaa gatatcccaa ccgttttatt aattggtatc tgtggctctt
   115381 ttattctttt aattagtagt tggcataaag ttatttccat ttataaattg atcagctaaa
   115441 tcagcatcat aatcagccgc gataaattgc gctgctgatt taataaatgc atccatatca
   115501 tgaacatctt tattagtatg tctacgaaca tagtgcgttt catcattacc aatccaagta
   115561 gcagctttgg ctaatctttg gagtttagga aaatcattca aataattttc aattgtttta
   115621 cctaataaca ttttttttat tttatcttca tcctctgaat ttttagatat tgcataatct
   115681 ttaattagaa attctagagc ttttctataa ccaactccag caatttgttc caattgctca
//
LOCUS       DAYYCQ010000002        82307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000002 DAYYCQ010000000
VERSION     DAYYCQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.002"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..196
                     /locus_tag="ACHAYF_000138"
     CDS             <1..196
                     /locus_tag="ACHAYF_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HFM8577609.1"
                     /translation="KKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFANGEQLEMG
                     KRSIDKLKNFMQEKIENESF"
     gene            372..1115
                     /locus_tag="ACHAYF_000139"
     CDS             372..1115
                     /locus_tag="ACHAYF_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8577610.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1108..2688
                     /locus_tag="ACHAYF_000140"
     CDS             1108..2688
                     /locus_tag="ACHAYF_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8577611.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2939..3418)
                     /locus_tag="ACHAYF_000141"
     CDS             complement(2939..3418)
                     /locus_tag="ACHAYF_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8577612.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3434..4186)
                     /locus_tag="ACHAYF_000142"
     CDS             complement(3434..4186)
                     /locus_tag="ACHAYF_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8577613.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4303..4373
                     /locus_tag="ACHAYF_000143"
     tRNA            4303..4373
                     /locus_tag="ACHAYF_000143"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4335..4337,aa:Gly,seq:ccc)
     gene            4734..5459
                     /locus_tag="ACHAYF_000144"
     CDS             4734..5459
                     /locus_tag="ACHAYF_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8577614.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5714..5980
                     /locus_tag="ACHAYF_000145"
     CDS             5714..5980
                     /locus_tag="ACHAYF_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8577615.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            5977..6408
                     /gene="ruvX"
                     /locus_tag="ACHAYF_000146"
     CDS             5977..6408
                     /gene="ruvX"
                     /locus_tag="ACHAYF_000146"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8577616.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6433..6738
                     /locus_tag="ACHAYF_000147"
     CDS             6433..6738
                     /locus_tag="ACHAYF_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8577617.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6819..7964)
                     /locus_tag="ACHAYF_000148"
     CDS             complement(6819..7964)
                     /locus_tag="ACHAYF_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8577618.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(8025..8672)
                     /locus_tag="ACHAYF_000149"
     CDS             complement(8025..8672)
                     /locus_tag="ACHAYF_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8577619.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            8866..9159
                     /locus_tag="ACHAYF_000150"
     CDS             8866..9159
                     /locus_tag="ACHAYF_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577620.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            9203..9514
                     /locus_tag="ACHAYF_000151"
     CDS             9203..9514
                     /locus_tag="ACHAYF_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577621.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            9511..10135
                     /locus_tag="ACHAYF_000152"
                     /pseudo
     CDS             9511..10135
                     /locus_tag="ACHAYF_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            10276..10434
                     /locus_tag="ACHAYF_000153"
     CDS             10276..10434
                     /locus_tag="ACHAYF_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577622.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            10435..10797
                     /locus_tag="ACHAYF_000154"
     CDS             10435..10797
                     /locus_tag="ACHAYF_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577623.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            10834..11682
                     /locus_tag="ACHAYF_000155"
     CDS             10834..11682
                     /locus_tag="ACHAYF_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8577624.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            11672..13147
                     /locus_tag="ACHAYF_000156"
     CDS             11672..13147
                     /locus_tag="ACHAYF_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8577625.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            13413..13820
                     /locus_tag="ACHAYF_000157"
     CDS             13413..13820
                     /locus_tag="ACHAYF_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8577626.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            13823..14038
                     /locus_tag="ACHAYF_000158"
     CDS             13823..14038
                     /locus_tag="ACHAYF_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577627.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            14042..14422
                     /locus_tag="ACHAYF_000159"
     CDS             14042..14422
                     /locus_tag="ACHAYF_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8577628.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            14556..14711
                     /locus_tag="ACHAYF_000160"
     CDS             14556..14711
                     /locus_tag="ACHAYF_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8577629.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            14780..15253
                     /locus_tag="ACHAYF_000161"
     CDS             14780..15253
                     /locus_tag="ACHAYF_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8577630.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            15250..16944
                     /locus_tag="ACHAYF_000162"
     CDS             15250..16944
                     /locus_tag="ACHAYF_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8577631.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            16910..17095
                     /locus_tag="ACHAYF_000163"
     CDS             16910..17095
                     /locus_tag="ACHAYF_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577632.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            17099..18274
                     /locus_tag="ACHAYF_000164"
     CDS             17099..18274
                     /locus_tag="ACHAYF_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8577633.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            18267..19790
                     /locus_tag="ACHAYF_000165"
     CDS             18267..19790
                     /locus_tag="ACHAYF_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8577634.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            19846..20130
                     /locus_tag="ACHAYF_000166"
     CDS             19846..20130
                     /locus_tag="ACHAYF_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8577635.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            20117..20452
                     /locus_tag="ACHAYF_000167"
     CDS             20117..20452
                     /locus_tag="ACHAYF_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8577636.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            20747..21373
                     /locus_tag="ACHAYF_000168"
     CDS             20747..21373
                     /locus_tag="ACHAYF_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577637.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            22115..22951
                     /locus_tag="ACHAYF_000169"
     CDS             22115..22951
                     /locus_tag="ACHAYF_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8577638.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            23015..23446
                     /locus_tag="ACHAYF_000170"
     CDS             23015..23446
                     /locus_tag="ACHAYF_000170"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8577639.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(23508..24830)
                     /locus_tag="ACHAYF_000171"
     CDS             complement(23508..24830)
                     /locus_tag="ACHAYF_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8577640.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(24984..26624)
                     /locus_tag="ACHAYF_000172"
     CDS             complement(24984..26624)
                     /locus_tag="ACHAYF_000172"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8577641.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            26957..27427
                     /locus_tag="ACHAYF_000173"
     CDS             26957..27427
                     /locus_tag="ACHAYF_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8577642.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            27649..28752
                     /locus_tag="ACHAYF_000174"
     CDS             27649..28752
                     /locus_tag="ACHAYF_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8577643.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            29036..29716
                     /locus_tag="ACHAYF_000175"
     CDS             29036..29716
                     /locus_tag="ACHAYF_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8577644.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            29806..30840
                     /locus_tag="ACHAYF_000176"
     CDS             29806..30840
                     /locus_tag="ACHAYF_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8577645.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            30854..31189
                     /locus_tag="ACHAYF_000177"
     CDS             30854..31189
                     /locus_tag="ACHAYF_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577646.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            31173..33653
                     /locus_tag="ACHAYF_000178"
     CDS             31173..33653
                     /locus_tag="ACHAYF_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577647.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            33709..35268
                     /locus_tag="ACHAYF_000179"
     CDS             33709..35268
                     /locus_tag="ACHAYF_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8577648.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            35265..36629
                     /locus_tag="ACHAYF_000180"
     CDS             35265..36629
                     /locus_tag="ACHAYF_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8577649.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            36800..37870
                     /locus_tag="ACHAYF_000181"
     CDS             36800..37870
                     /locus_tag="ACHAYF_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8577650.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            37885..38553
                     /locus_tag="ACHAYF_000182"
     CDS             37885..38553
                     /locus_tag="ACHAYF_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8577651.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            38573..39151
                     /locus_tag="ACHAYF_000183"
     CDS             38573..39151
                     /locus_tag="ACHAYF_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8577652.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            39552..40463
                     /locus_tag="ACHAYF_000184"
     CDS             39552..40463
                     /locus_tag="ACHAYF_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8577653.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(40551..41468)
                     /gene="rnhC"
                     /locus_tag="ACHAYF_000185"
     CDS             complement(40551..41468)
                     /gene="rnhC"
                     /locus_tag="ACHAYF_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8577654.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            41670..42107
                     /gene="zapA"
                     /locus_tag="ACHAYF_000186"
     CDS             41670..42107
                     /gene="zapA"
                     /locus_tag="ACHAYF_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8577655.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            42166..42714
                     /locus_tag="ACHAYF_000187"
     CDS             42166..42714
                     /locus_tag="ACHAYF_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8577656.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            42829..45189
                     /locus_tag="ACHAYF_000188"
     CDS             42829..45189
                     /locus_tag="ACHAYF_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8577657.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            45276..45590
                     /gene="trxA"
                     /locus_tag="ACHAYF_000189"
     CDS             45276..45590
                     /gene="trxA"
                     /locus_tag="ACHAYF_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8577658.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            45772..47550
                     /gene="uvrC"
                     /locus_tag="ACHAYF_000190"
     CDS             45772..47550
                     /gene="uvrC"
                     /locus_tag="ACHAYF_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8577659.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            47695..48594
                     /locus_tag="ACHAYF_000191"
     CDS             47695..48594
                     /locus_tag="ACHAYF_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8577660.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            48587..49849
                     /locus_tag="ACHAYF_000192"
     CDS             48587..49849
                     /locus_tag="ACHAYF_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8577661.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            50010..51908
                     /locus_tag="ACHAYF_000193"
     CDS             50010..51908
                     /locus_tag="ACHAYF_000193"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8577662.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(52238..52957)
                     /locus_tag="ACHAYF_000194"
     CDS             complement(52238..52957)
                     /locus_tag="ACHAYF_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8577663.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    53147..53194
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            53294..53626
                     /locus_tag="ACHAYF_000195"
     CDS             53294..53626
                     /locus_tag="ACHAYF_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577664.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            53613..55616
                     /locus_tag="ACHAYF_000196"
     CDS             53613..55616
                     /locus_tag="ACHAYF_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8577665.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            55635..56105
                     /locus_tag="ACHAYF_000197"
     CDS             55635..56105
                     /locus_tag="ACHAYF_000197"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8577666.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            56126..56710
                     /locus_tag="ACHAYF_000198"
     CDS             56126..56710
                     /locus_tag="ACHAYF_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8577667.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            56725..57711
                     /locus_tag="ACHAYF_000199"
     CDS             56725..57711
                     /locus_tag="ACHAYF_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8577668.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            57701..58012
                     /locus_tag="ACHAYF_000200"
     CDS             57701..58012
                     /locus_tag="ACHAYF_000200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8577669.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            58034..59815
                     /locus_tag="ACHAYF_000201"
     CDS             58034..59815
                     /locus_tag="ACHAYF_000201"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8577670.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            59808..61184
                     /locus_tag="ACHAYF_000202"
     CDS             59808..61184
                     /locus_tag="ACHAYF_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8577671.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            61191..61826
                     /locus_tag="ACHAYF_000203"
     CDS             61191..61826
                     /locus_tag="ACHAYF_000203"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8577672.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            61856..63211
                     /locus_tag="ACHAYF_000204"
     CDS             61856..63211
                     /locus_tag="ACHAYF_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8577673.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(63403..64797)
                     /locus_tag="ACHAYF_000205"
     CDS             complement(63403..64797)
                     /locus_tag="ACHAYF_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8577674.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            64889..65860
                     /locus_tag="ACHAYF_000206"
     CDS             64889..65860
                     /locus_tag="ACHAYF_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8577675.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            65857..66639
                     /locus_tag="ACHAYF_000207"
     CDS             65857..66639
                     /locus_tag="ACHAYF_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8577676.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(66808..67179)
                     /locus_tag="ACHAYF_000208"
     CDS             complement(66808..67179)
                     /locus_tag="ACHAYF_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8577677.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            67313..67756
                     /locus_tag="ACHAYF_000209"
     CDS             67313..67756
                     /locus_tag="ACHAYF_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8577678.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(67799..68608)
                     /gene="yidA"
                     /locus_tag="ACHAYF_000210"
     CDS             complement(67799..68608)
                     /gene="yidA"
                     /locus_tag="ACHAYF_000210"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8577679.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(68627..69994)
                     /locus_tag="ACHAYF_000211"
     CDS             complement(68627..69994)
                     /locus_tag="ACHAYF_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8577680.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(69978..70808)
                     /locus_tag="ACHAYF_000212"
     CDS             complement(69978..70808)
                     /locus_tag="ACHAYF_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8577681.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            70971..71408
                     /locus_tag="ACHAYF_000213"
     CDS             70971..71408
                     /locus_tag="ACHAYF_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8577682.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            71484..72104
                     /gene="rpoE"
                     /locus_tag="ACHAYF_000214"
     CDS             71484..72104
                     /gene="rpoE"
                     /locus_tag="ACHAYF_000214"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8577683.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            72293..72799
                     /locus_tag="ACHAYF_000215"
     CDS             72293..72799
                     /locus_tag="ACHAYF_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8577684.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            72816..73715
                     /locus_tag="ACHAYF_000216"
     CDS             72816..73715
                     /locus_tag="ACHAYF_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8577685.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            73802..74686
                     /locus_tag="ACHAYF_000217"
     CDS             73802..74686
                     /locus_tag="ACHAYF_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8577686.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(74878..74949)
                     /locus_tag="ACHAYF_000218"
     tRNA            complement(74878..74949)
                     /locus_tag="ACHAYF_000218"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74914..74916),aa:Arg,seq:ccg)
     gene            75009..76343
                     /gene="rpoN"
                     /locus_tag="ACHAYF_000219"
     CDS             75009..76343
                     /gene="rpoN"
                     /locus_tag="ACHAYF_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8577687.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            76492..77583
                     /locus_tag="ACHAYF_000220"
     CDS             76492..77583
                     /locus_tag="ACHAYF_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8577688.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            77624..78625
                     /gene="gap"
                     /locus_tag="ACHAYF_000221"
     CDS             77624..78625
                     /gene="gap"
                     /locus_tag="ACHAYF_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8577689.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            78735..79925
                     /gene="pgk"
                     /locus_tag="ACHAYF_000222"
     CDS             78735..79925
                     /gene="pgk"
                     /locus_tag="ACHAYF_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8577690.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            80029..80784
                     /gene="tpiA"
                     /locus_tag="ACHAYF_000223"
     CDS             80029..80784
                     /gene="tpiA"
                     /locus_tag="ACHAYF_000223"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8577691.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            80900..82198
                     /gene="eno"
                     /locus_tag="ACHAYF_000224"
     CDS             80900..82198
                     /gene="eno"
                     /locus_tag="ACHAYF_000224"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8577692.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        27411 a        14140 c        17169 g        23587 t
ORIGIN      
        1 taaaaaatca ctcgaatatg agtatttatg taaagattta aaagcttata tcattaatac
       61 tgaaaacatt acatctatta gcagagtaaa cgaaatagta acatttgcaa atggagaaca
      121 actagagatg ggaaagagaa gtattgataa actaaagaat tttatgcaag aaaagataga
      181 aaatgaatca ttttaaagaa aacaaagact catatcttac ctgttagaag agtgttttcc
      241 tgtagaagag aacaacccaa gtagcagcct agtcgtttct atcctttttt gttatgcact
      301 ctttttgaca tttactccac caataacgaa gggtacaata aaaagtaata acaaacaaaa
      361 ggagtacaga aatgattgaa ttcatcaatg taagtaaaag ttacgggaaa aaacaagcat
      421 taaaagactt gtctctctcg ataagacaag gggaaatttt tggattttta ggacataatg
      481 gtgccggaaa atcaactacg attaaaagtt tagttagtat tattgaacca tcatcgggaa
      541 cgattttagt agatggaatg aaattgacgg aaaatcgatt gagtatcaaa caaaaaatcg
      601 gttatgtgcc tgattcccca gatatttttt tacaattgac ggctggagaa tattgggact
      661 tgatttcagc tgcctatgaa ttagacacac aaaaaaaaga aaagcgttta gcagaattga
      721 cagcgctttt tgatatgtat agtcatcaaa atgaaacaat cgcaagtttt tcacatggaa
      781 tgcgtcaaaa aacgattttg atcggcacgt tgctgccaga tccagatatt tgggtattgg
      841 atgaaccttt gcaaggactt gatcctcaag cagcatttga tttgaaagaa atgatgaagg
      901 ctcatgccgc aaaaggaaaa acagtcattt tttctacaca tgtattggat accgcacaac
      961 agttatgcga taaactggct attttgaaga aaggtgaatt gatttatcag ggggcagtta
     1021 gtgaattatt gaatggctca cctgatgaaa cgctagaaaa gatttatttg aaaatggctg
     1081 gaaggcaagc gacaggagat ggaccgtatg tataagcgac aattattaga attaattcga
     1141 gttaatcttt tatatgttaa tccacagctc acgaataaag caagagagaa aggaaaaaag
     1201 ggaaagcgat taactatcta tcttttgtct caaaatcttt tttcagggct gattttttta
     1261 ctgatttacg gatttgctat gtttgctatg aactttgtta aattgccagg tttctttact
     1321 tattacgtgg cactttttgg tatgttaggt ttaactcaaa gtatatcagt catttataat
     1381 atcttttttg aaggaaatga cctgcaagta tttttgcctc tgccttttgg acaagaacag
     1441 gttttttcag caaagatttt ggtagttgct ttgaccatca ttccttttgt ttttccgctt
     1501 ctagtcatgt ttattttaac cggatggcga gctgatctag ggattttgct tactatcatt
     1561 atctcaatcc ttttgttttc tctatttttg attattcttt tttgtctggc tagtttgatt
     1621 gtttttggtt tggctcgaac agcagtgttt aaaaaacata gaaaaacagt ttctagtatc
     1681 ttgcttggca gctcgatgct gatcgcattt gctggaatca tgtggatgaa tcatcaaacg
     1741 gggactattg aaaatgagtt aacagatcgg catccgattt cgatattatt gccattctat
     1801 gatgctgcca gtcagccact ttctcaacaa ggtttattgg gttggggaat actagctatc
     1861 acggctctgg gatttttcct tgctgtaaaa cagctgatag taccaaagct tacagagcaa
     1921 ctgactgatt caaacagtga gttcaaaata aaaagaaccc ataaaaagaa ccaaaatgtg
     1981 catcagcttt tatttagtta caatgctcaa ttagtaaaag aacctaattt gattatgcaa
     2041 gttctttctt cttccttgtt aactccaatc atttttattc ttgcttttgc cctaggaggc
     2101 gaaataaaat taactgattt agataataag ttaatcggtg ttgttttcct ggtagggatc
     2161 gcattggctt tcttaacagt taatcagact tcttttatca gcaatcttat ttctttggat
     2221 caagaaaatt ttctgtttat ccgttctttg ccaatctcta tgaaccaata tttaaaagaa
     2281 aagttcagat tcggatggat attgcagagt tttttaactg gtgggatcgc attattaagc
     2341 ggactgtctt ttcagctgcc tttgtttttt atccctagct tgctatttgg ttctttattc
     2401 ggctgctact tattgtcact acgttatttc gctagagatt atcggttgct gctgctgaac
     2461 tggaccaata ttaaccaatt atttaacaga ggttcaggaa gtttgggttt agtcgcaact
     2521 atgatgggtt cattgatcat cagtattatt cttatcgtct tatacggaat gttggctttg
     2581 ttttatccat tttggttagt gaatattcct gtgatcggtt tagttctaat actttccgct
     2641 ctatggatcg tttattatca acgaacgttt tggaaaaaat ttgaataaag aagaggggcc
     2701 gtgacaaaaa tcatgtcgca gccccttttt tacagataga gggtgttcaa aagtcagttt
     2761 ctcagtatta agtcaatttt atgtaaaaat gtgagaagct atttttacaa aacttttttt
     2821 aatactcata attacttctt attgctcctg cggttacttg tcgcaatgtt tgtgagatat
     2881 tttgtcatcg ctcaaggctg aacgtctctt tcacaacctt ttctttgtta cacttcgttt
     2941 atttaaaaag ccgttttttt cgtttcatca tgattgcgtc acgctttact ttgaaattac
     3001 ggatgttatg gttaaagact tctgataata caagacctaa agcgattgct ccagcaatca
     3061 taattgcttg gaccgcataa tgagccgctt ctcgatagga ttcagtaacg agatttcgaa
     3121 ccgcttgata agctaaacca ccagggacta gaggaaccat cccgggaata ttgaaaatcg
     3181 ttacgggcat tttcaaacgt ttagagaaaa gatcgctgat gaatgctact cctaaagaac
     3241 caagcaatga accaaaagca gcagatccgc ctatttgaac acagatccag tagatcatcc
     3301 agccaaaagc accagaaaga ccacaacaaa tcaatgaacg cctaggtaca tttgtaataa
     3361 cggcaaatgc cgcagtcgct aaaaaactaa aagaaaactg aataaatatc gtccccatac
     3421 ttgtgttcct ttcctaataa aaaagctgga aaataaaagc aatcgcaaaa ccgatcatcg
     3481 tagcagtcat catcgcttct gttccgcgag aaactcctga gacataatgt ccagctagca
     3541 aatcacggat agcgttcgta atttgaactc caggaactaa aggcatcaca catccgataa
     3601 tgattagatc ctgattgctt cctaagccaa aacgtacgct gtagatagcg gctatgccaa
     3661 tcaataaaga agataaaaat tccgataaaa atttgattct aaaaaatttc aaactatata
     3721 gataagctgc gtatcccaat cctccaatga ctaaagtcac aggtaaatcg gtcatctttc
     3781 cgccaaataa caccataatt gttccactga tcactgccgc gctaacgatt tgcaaccaga
     3841 caggaaagaa agaacggtct cggtcgattg cttgcagttc gcagtaaagt tcttctaatg
     3901 ttagctctga agcaacatat ttacgtgaaa gatcatttac ttttactact ttttctaaat
     3961 taatcgaacg gttggtaatt tgttccattc ggatggtcga ggtacgatca agaccaataa
     4021 agagaccggt ttgagtgaca tagctgacca aacggaaatt tccagaagct aaagcaattc
     4081 gactcatcgt atcttctacc cgatacatct ccgcattgct ttccatcatg attttcccag
     4141 cgagtaaaca agtgtccagc aacagatcta attcttttcc cgtcatagca tcttctccaa
     4201 taattgattg attagtttta ttttactaca aaacatacca gaagcgtaaa gaataaaaaa
     4261 acttgaggaa ggaataaaag aggcgtatac tgtttttgta ttgcgggtgt agtttagtgg
     4321 taaaatccca gcttcccaag ctgatgtcgc gggttcgatt cccgtcaccc gcttaaaata
     4381 ggttgtgaaa aagctgctct gcaacaagta ataagaacag ccaagcaaaa acaacttctt
     4441 atgtttttaa cttggatgga cttattaccg cagatgcaag cttttgcgca ccgtttacca
     4501 agcggttgtg aaatcagtgt attgtcttga gtatgaagaa aatttttgaa taataataaa
     4561 taccttacag atagagtggt gactgattag agacgaggaa tccgtctctt ttttgtattt
     4621 ttaagccatc ttaaaacagg ttttattttt tgctgtttct atttctttct agtatttatt
     4681 tataggggaa catatgttaa gatttaggga gctgttagta aaggagtaaa gaaatgagca
     4741 aaaaaacaac caaggcgatc gtggggatcg tcgctgctgt gattgcaggg gcattcggtg
     4801 tttccaccac acaaaaagat tcagatatct ttcagcagat tacaactatt tttgattcca
     4861 caccacaagc ttctcaagta gcggatgtgc cagaatatga tggaaaacat caagaaatag
     4921 aaatcaatgg gaatcagccg gaatttacaa aagaggatct tagcctttcc aaaggaacat
     4981 gggaaagcta ttcaaatatt gataaattga acagagtagg acaagccaat gctatgcttg
     5041 gaaaagattt gttccctact gaaaagagag agcctttgta tattgacccg acaggttgga
     5101 aacaaaagaa attaagtgac gggcaatggt tatataatcg cagccattta attggtttcc
     5161 agctgactgg tcaaaataac aacattaaaa acttgatgac agggacacgt tcgttgaatg
     5221 ctccatacat gcttgctcac gaaaatgata ttgcggcata tatcaaagaa acaaatcatc
     5281 atgtacgcta ccgtgtcact cctcattttg aggggaatga actggtagcc aggggagtgc
     5341 aattggaagc agaaagcatt gaagacaaaa aaattgaatt caatgtcttt atctacaatg
     5401 ttcaagatgg ctacacgatc aattatgaga caggacaagc aacaaaaaca aaaaattagt
     5461 agcagctatt cgaatttttt atgaatatta tgaaaaaatt gtgtaaaaac atacattcaa
     5521 agaaatatag cgtatacttt atgaaaagag tacttttgat aaagctacag aaaaaaggaa
     5581 aaaaacatac aaatagtgtt tcttaattta ttcggaagtt tggaatattt gtttgtagtt
     5641 ttccctttct tcgagtaaaa taaggttgta gaaggtaagc tggaattacc ttgcgggaaa
     5701 gaggtgtaga atcatgggtt ttacagatga aaccgtacgt tttggttttg acgatagtcg
     5761 caaaaaagaa gtgagcgaaa cattagcgat tgtttatcgg gctttggaag aaaaaggcta
     5821 caatccaatc aatcagattg tcggctactt gctttctgga gacccgtcct acattcctcg
     5881 ttatcgtgat gctcgaaatt tgattcgccg tcacgaacgc gatgagatca tggaagtaat
     5941 tgtcaaggac tacctctcaa atcatggagt gaatttatga gagtaatggg cttggatgtc
     6001 ggttctaaga cagtaggtgt tgctatcagt gacccctttg gctggacagc tcaaggagta
     6061 gagatcattc ggatcaatga agcagaagaa gagtttggct ttgatcggtt gaaggaatta
     6121 gtagcacaat atgaagtaga ccggtttgta gttggattac ctaaaaatat gaacaattcc
     6181 atcggaccaa gggcagaagc ttccatgtca tacggagaaa agatcaaaga actattctcc
     6241 ttgccagttg attatcaaga tgaacgatta accactgttc aggcagaacg tatgctagta
     6301 gagcaggcaa atacttcaag agccaaacga aaaaaagtca tcgataaatt agcagctgtg
     6361 atgatcttac aaaattattt agacggctca ggtaagcaat tattttaata ataaaagaaa
     6421 agaggtaatc ttatgacaga acataaccat tcccacgacc atgaaggaca cgaacacatc
     6481 acattggtgg atgaccaagg aaatgaaaca ttatatgaaa tcctactaac agttgacgga
     6541 caagaagaat ttggacgtaa ctatgttctt ttatatcctg ctggtgtttc agaagatgaa
     6601 gatgtagaat tacaagctta tgcatacatc gaaaacgaag atggaacaga aggcgagtta
     6661 gaacaaatcg aaacagacgc agaatgggac atgattgaag aagtattcaa tacattcatg
     6721 gcggaagaag aagaatagac tataaagcta ggagaaacct tgatatgata gggtttctcc
     6781 tttttttatt ttttactgta tgccatattg tatgccatct agtcaatatt taagtgttgt
     6841 actaattttt caacagtctt ttctttcgtt tcttttgcca agtgaccgta acaatcgatc
     6901 gtctgactaa tttttgtatg ccgtaatcta gtttgaactt ctaccatagt agctccagcg
     6961 tccaagagca ttgaagcatg agtgtgtctt gctacatgca caccgagaaa tggaaccata
     7021 gctttttcac aaatagtaat caatcttgca taaatagcgt ggtgtgtgat tagttcttta
     7081 tagataccac aaaaaatcat agtcggatca gttacaccaa gtgtcagcat atatttccgc
     7141 tgattaatct gccatttttt taatatagaa attgttcttt catctagtgg aagaacccca
     7201 gcactacaat cagttttagg atcactgatt ttatagccat ttttggattg gcttagtgtt
     7261 ttattgatgg tgaccgtatt ttcttgaaaa tcaatgtctg accaattcaa tgcaagggct
     7321 tcactgatcc taaccccgct ataaaagaga aagcgtagta acgttttatc aaagtcgttt
     7381 cgataagttt caggtttgct gtttaggtaa tcaaacaagg tgatcagttg ttctttactg
     7441 taaactttga ccttttcttt atgcttagtg gtggttcttt taggcattaa tacgttgtct
     7501 agtggattag tctcaataat cccaagattg atagcgtagc taaaaatacg cttagaagtt
     7561 gaatgaagta ccttgtattg gtcagtagtt tcagcccagc tatttacaat ttgttgggct
     7621 ttttttaccg tgacttcatt cacacgaaga tttccatatt ttggaagaat ccactttcta
     7681 aacttgattt cagttttcat gaaggtggct tctcggacag tggttttata tgtttctagc
     7741 cacaagtcat aaacttcttt aaacgtttcg ttgttcgctt ttttaatacc gtttttatcg
     7801 tagtccactt gcaagcgtga caaggcaagc tgggcttctt tttttgtttt gtaacctcgt
     7861 ctacgaacct tatcaggttt gccagtcatt ttgttcattc caagataggc ttcatagagc
     7921 caaaatttac cttttttagt ttcatattgc ttgaatgttg ccataatagt tttccctcca
     7981 tagtgtagca agccgtgggg gcggttggag gaggcagttt attattattt tgaatagtgt
     8041 ttatttagct cagtaacaag agctgtgaac tcttttagaa gatcattttc ataaaattct
     8101 tgatactctt ttttctcatc tgtatttttt tctgaatcat taattaatga gtcatatcgt
     8161 aaaagcgtat ttagaagagc tgcgagataa gcaattgggg tactcccgtc aggaaattca
     8221 attcgagatc ctgttttaag atattttatt gcgtttgtta aataaatctt ctgtgagaca
     8281 gtcaggttat cttcttcgat gttattgata gcattagaaa gattagaatc aattatagag
     8341 ttattttttt caatagtttt tttcaattgc ttttcaagca catttcgctt aatttcgggc
     8401 atcatatgca tatctcttaa tgtataagat ccgtttaata gataggtagt ggatacatct
     8461 aatacctttg aaagttgttt aagcctggtg ttattgggaa gattatagcc attttcccaa
     8521 ttactaacgg cacctttact ggccggtggg gataatttac ctccaagttg ttccattgag
     8581 agtccctttt caattctagc cagtctaatt ctttgtcctg tctccactgc ttcggggtat
     8641 ttttttctag ccacattttc accaccattc atatattttc tctactatat cacgatttaa
     8701 atatgtgtga aacataaaag ttcaaaaaag tatgttaaaa ttcttgcaaa tgaaaaggta
     8761 tgatggtata ttgagtttat aagaggtata gaaatgtatt caagagcctc ttgcattttt
     8821 ttaaaatctt tgtatagaaa tgtatgctaa cgagggggga taaatatgga attaaaaatt
     8881 gacaaactca cgcttgatga tccagctttt aaaattattg acgatcgagt tgttcaacaa
     8941 gtagagcaaa ctcaaggaac gttactttca ttattagaaa aaaagtacca gtttccaaaa
     9001 tatatgaaca aggcacaagc tgctaagtat atgaacgttt catacaatac tatgatgcag
     9061 aaatatgttc ctaacggttt aaagttgatt atagttgatg gagtcgtaag aatttcacaa
     9121 gatgaatgtg atcgctttat ggaagaatac caaaaataaa cgcaagccgt gggggcaaag
     9181 ggattaatag gaggaaataa taatggaatc gacacaaaat aaagcaatcg aaaaagtatt
     9241 atcaacaatt cttacagaag acacagcggc agaattagca aacttggaag gcaaagcgct
     9301 agaagaaacg tttgaatgtt tgtacgaaca aatggactat caaaaattgc taccacaaga
     9361 accaacggta agcggagtat tgcgtggctt gaacgattta gtacaagcag agtttaaaga
     9421 acgactttct attgaagagt atcaagaaat tttgtatcag caagttgagt tgttagcgag
     9481 cttgctaggg cttgaactgg aggacgttga atgaaaactg aaatttggaa cggacacatt
     9541 ataagatttg tcgatattaa tgatgaatgg tgggcagtgg cgaaagatgt tgcggaagcg
     9601 ttaggtctta aacaggttac tagagccatt cactctttgc ctaaagatgg ggttactact
     9661 agtaaggtca tcgattcatt gggcagaaca caagatgtaa acatcatcaa cgaaaaaaaa
     9721 tatttaccgc atggcattca aaagccgtaa aaaagaagcc gaggcatttc aagactggat
     9781 tttcgacatc atcaaagaac tacgccagtc cacaggactt gaaggctttc aagtattccg
     9841 catgctagac aaagaacacc aaaaagaagc aatgactaaa ttaagccacg ctattactga
     9901 accaaaacca gtagactaca ttaaagcgaa tgtgatagcc aataaagctg tatcaactat
     9961 ttatggtcat tccaaaatgg taaaaaagaa agatatgact cctgaaatgt tggttgatcg
    10021 tgaacctatt ttagatgaga cagtagaatt aatgactgtt aaagaaaaat atgggttgca
    10081 gttttcagtg agtgagaaaa tttataatcg ttctgctgaa ttgcagacaa cttaggagca
    10141 ttaacatgat cgagaacaaa gaaaaagcct tatccataac agtcttggcg gacaggataa
    10201 aggcaactta attgggataa aacccgatat tctttgttcc gattatacca tattaaaaaa
    10261 tcggagggaa ccattatgaa acgaaactta attataggaa gcattttaat cggcggaatt
    10321 ttcggcttat taccattaat tgcaaaaata gctatctttg caggattagc gttatatctc
    10381 ttcactgctt atgacgagta cgactatcaa ttaagaactg gagggcataa gtagatgtac
    10441 aaaacaaaac tattaaacca actcgatagc cttgaattag aggaaattaa tcaagggatc
    10501 gctgaactag aaaataatat cgggaaaacc tactttggaa attcattcaa tgaaaaacta
    10561 acagttttgt atgtactcaa aaaacatgca gaacacaaaa taatttgtcg agaaatcaac
    10621 gagctaaaaa atcagatttt aaccgcatgg ttaaacatta ctgatatgca agaagcaagg
    10681 gtgaaaacat ttaatacatg ggtaaagtat cagaatcaat taaaaggtgc tgaatttgtt
    10741 cgggatgggt tgaaatacga acttgagcag ttgaagctca tggaggtgtc tgaatgattt
    10801 ataccagtat tttgaaaaag gaggagaggt ttattggcaa ataattatca agcagcaacg
    10861 gaattacaaa agtcgggatg gtcaatttat cccttatctc ctggaactaa aattccaata
    10921 aaaggctcta gtggttggaa tgatgcaact aacgattcta atcaaattaa tgaatggtgg
    10981 acggaagatg caactagaaa tattgggtta atgcttgaag atcatcaaat ggtggtagtt
    11041 gacctagatc aacattctga aacacgaaac ggcgttgaga attataaaaa acttgcttca
    11101 atgtatgatc catttccttc tacttatacg gaaatcacac caggtaaagg aatccatttc
    11161 ttctttaaaa ttcctgatgg tgtaattatc caacaagaaa caggtgcatt tgctgatatc
    11221 ttcgggcgtg atgataaagg taaaagttta agcgggattg atattatcac taaaggagtt
    11281 ccaatagcgc caactatgat tatttcaaga agtgttggga gagcatatga acctattgaa
    11341 aacgtatctc taaataatat tgcaccagct cccgattggc tagtgaacct actgctaaaa
    11401 aaaccgcaag aaataaagac tcaataccga cccgtagcaa aaacagggac agcaaaaaaa
    11461 ttagatatga ttgttcaagg cgctggcgaa ggtagtagaa acgatcattt aactaagtta
    11521 tgcggttggt tactatggca cggtgtagat aatcaaactt tagcagaatt gatttataca
    11581 gcaaatgcgt acaatgaacc accgctaaaa gataaagaag tgaatcaaat tattcgatcg
    11641 atgattaaaa aagatttgag ggggaaagct catggctcat aagcataaat tagatagtat
    11701 tttagacttt cccgaagcca gcgagcgtga agacaatatc attgaattga aaacctggat
    11761 gtcacgatta agatgcaata aagacgatca gattaaaagt aacagtgttg tgaatgcaga
    11821 attgatttta accaatgata gcaatttagc tggaacaatt gcttataacg aatttagcgg
    11881 atacattcac ctattaaaag attctccttg gatcaatcgt accgctggag aatgggaaga
    11941 cagttttgag gatgccttaa cggcatatat tgaagaaaac tacaatgtag tttttgatga
    12001 caacaaaata cacaaagcag tagtaaatgt ggctagaaag aatgttttca acccagtaaa
    12061 agaacgtatc gaaaaagtta aatgggatca acagcctaga ctggaaacac tgtttattga
    12121 ccttttaggg gtagacgaca acctttacac acgagaagta acaaaacgtt ggatagtcgg
    12181 atcagtcgca cgtatctata aaccaggaat aaagtttgag atcgttccag ttttggatgg
    12241 accgcaaggg attggtaaga gtaccgttcc agcattactt tacacggatg attttttcac
    12301 cgattcatta gattcgctag gtgaaaagaa agatgattat atgcagcttc aaggcaatgt
    12361 aattattgaa cttggagaat tatcttcaat gaacaaaact aaaattgaac agataaaaaa
    12421 cttcatcagt gccaagatcg ataaaattag acctccttat ggtcggaatg tcatagcttg
    12481 ggcaaggcaa tgtgtattta ttggaacgtc gaatgatggg cagtatttga aagatgatac
    12541 aggaaaccgc cgtttttatc ctttgccttg taagaataag ccaaagatga atccgtttaa
    12601 aacaaaggat gaatattttc ttcaagtgtt agctgaagca aaggtgcttt ttgataaagg
    12661 acaacgaatc tattttagtc ccgatgaaga taaagaagtc ttagaaattg caaaagacta
    12721 tcaagaggat gccaagatgg agaatccaat taaagaagca atcactaaat atttggaaat
    12781 ggaaatcccg tataaatggg aagaagctcc agcgtgggcg agacgttctt attatcagaa
    12841 ctatcctgac agtaaatgta atgagaaaaa tttacagcat ttctataatg gagctactat
    12901 gcaacaagag ttttatttag ttgatagcgt actcaccgct gatattttgg aagctgtttt
    12961 tgataaacaa gcaaaggatt tattgaatgg tcgatcagat gcagaaacta aaaagattgc
    13021 cttgattatt agcagtattc ctggttggga acggaagcaa ctgccaaaaa gaaataaacg
    13081 acgaggattt tacaataagt caaatgccta tgaaaataaa aatcgagctg gaaagtacaa
    13141 aaagtaacgg gtagtaacgg gtcagtaaca cctagcccgt tactagttta cccttactgc
    13201 cccaagggat acagcaattt ggtaacgggg taacacctta tttccttaaa acttttgaaa
    13261 tagttagcta atatgctggg gaataatgtt actggatttt acccgttacc tgttaccaaa
    13321 acgctagaac ccttgtcccc gttgggattg agtggtaacg ggtacctgtt acgtaggtgt
    13381 taccacgtgt tacgaatcaa ggaggaaagt aaatgctcaa tattatttca acaaacaaag
    13441 caccgaactt tcaatataca gatgaaatgg atcggtttct aatgaacacg ctggcattta
    13501 gtgtgggatt ggtaacagaa gattattcca catttgatcc agaagtatta aagataatgg
    13561 aagaagaacc tgattggctg caggagtcag tagcgtggtg tcaatcgcta gtagtgggat
    13621 cattggtaga tagtggcaac tatgatgata caggtgagct aatggatgaa tttaattgtc
    13681 tgcttaacct atatgatcga gcgagacaac gagagcttac atcgaatgag gataatttgt
    13741 ttttaaacat ccatgataag ttcttggctt tgctactaac agatgatgaa ttgataacta
    13801 atttattgga ggtagaataa ttatgcatat ggaaagtatt agatacttcg atggtgagaa
    13861 acacattgag tattcaaaca ctcaagaaga tgtagacttt attagttttg aggaagataa
    13921 aatcactaaa gttaatttta aagacggaac gtgcttgaag atagtttcgc cttatattga
    13981 atacaaatca aaatgggaac aagatgattc tgacgaacga gatcgctttg gttggtaatc
    14041 tatggcagtt aaaaaacaat gtaatcatgc tggatgtaaa acattgattg attataggca
    14101 gaagtattgt gggaagcata aagcaaagca aacggcaatt aagcgtgaag aaagaaagca
    14161 aagcgaaggt aaatattttc aattctatca aagtagaaca tggcgaaagg cttcatactt
    14221 gtatcggtta aatcatccag tatgtgagga ttgcctagaa gaaggcttga taaggaaagc
    14281 tgatgttgta gatcacaaaa ttgaactaaa agatgattgg aatagacggt tagatgaaac
    14341 taattttcgt tctttatgcc atgcacacca taattccaag acagccaatg aaagacaaag
    14401 acgagaaaag agtacccttt gagttaaggg ggcatagtgt gaaacttact gacaatcgat
    14461 gcctactcat tttggtacaa ataaccgttg tgaaaaggca taagggtaat tacaaataac
    14521 gattacactt gtaatcacaa gtgaaaaagt atataatgaa agtaggagat ttattgaaaa
    14581 atacaattgt tattactgat aaaacaggga aacagcgcac cattgagtta cctcattttg
    14641 gtcaagtgac cattcaaatg cagaacggaa aaatcattta tatagataaa ttagataaag
    14701 aaaaattctg atcgaaaaac gaaggaatgt gagcttaatt gcttacgttc ctttttcttt
    14761 tgtctgaaag gaggacaaca tgggacaccc taaaatatta cacgatacaa aaggcaatat
    14821 atcaagtgaa gaaaaagcaa tccgtgtgga cgctagagaa gaattgttca agcagcaacc
    14881 attaataaat atcacgcccc ctgactggat ggcagcgagc gctagaagtg aatggaatcg
    14941 tatcgtacca acattaaaaa aagattatcc attgagtgaa gcagattatg gttcattggt
    15001 agcctattgt ttagcctttg ctcgaatgaa aacagccgaa gccgagataa gaaaatcagg
    15061 tacgtttatc acatgcgaaa acggagtaaa gaaagccaat ccagcggttc gggttcaatc
    15121 acaagctatg agtgatttga aaaaacaagc cacctcacta ggtatgacct tagaatcacg
    15181 atcaaaacta gctttgaaca aggttaaaaa tgatgaaccc gaagacccgt tcaaagagtt
    15241 gatgaattca tgaatgatta cattgagaaa gtcctatcag gtaagctgat tgcacccaag
    15301 aaaattatcc aagcgtgtga gcgtcatata agcgatttgg agcgttctaa gtcagatagc
    15361 ttcccttatg tgttcgatga agaacaagcc accaaagcga ttaaattcat tgagttgcta
    15421 ccatctacgg acggtaaagc aatcaagatg ttaggatttc aaaaattcat tctaggcagt
    15481 ctttatggct ggcgtactaa agaagggaat tacaggcgat tcaatcgagc gtttaccagt
    15541 atgagccgta agaatgggaa aacgtatatc gcaagtggca tggctgccaa tgcgttgatt
    15601 atggaacaag aaccagcaga agcaaggcaa gtattgtttg taagtaacgc tttgaaacaa
    15661 gctaaattgg gctatgatat gctgtctaat tcacttagaa acgtggtcaa gtctagtaag
    15721 tttttaagac cacaactgaa aattatgaac tctaagattc agcacttgcc ctctaattcg
    15781 ttcgctatgg cactggctag tgaaaccagc acgctagatg ggtttgcacc aacaaccgca
    15841 attcttgacg agtggcacga agcaaaaact cgtaaaacgt acaacgtcat taagtcagga
    15901 atgacccaac aaaagaacgg cttattgtgt gttattagta ccgctgggct tgatttaaac
    15961 gttcctatgt acgaggaata cttattgtta gatcgtgtgc taaaaggcga agaacaagcc
    16021 gacaggtact ttatagcgat atgggaattg gacgatcccg aagaaattca cgatcaagag
    16081 aaatggatca aagccaatcc gatttttgaa agtgaagaaa tcaaaaaagt aatgattcca
    16141 accattcaag atgatgtgaa ccttgctttg aaacaaaata accttaattc tgtattggtg
    16201 aaaaacttca atttatggag acaagcgagt gaggacagct atatgattgc tgaagactgg
    16261 caggcaaccg aagtagaacc acaagatatt acaggcaagc ccgtttatat cggagtggat
    16321 ttatctaaaa cagatgattt aactagcgtt tcatggatcg taccgctaga taacggcgaa
    16381 ctttattgtg attcacatag ctttgtagcc accaaatatg ggcttcaaga caaagaaaag
    16441 cgcgacggtt tgccttatcg agaactagaa aaagctggtg agtgttccat tactcaatta
    16501 gaaagtggaa tcgtggacta tgaccaagta tttcagttta ttcaagattt gattcaagaa
    16561 aatgatttgg aatgtatggg gatttgttat gacccgtata acgctaattc gcttatcagt
    16621 aaagctgaaa aagccaacta cccaatgtta gaagtaagac aaggaacgat tactctaaac
    16681 gttccgaccc gaacttttag agaacaagtt tatgaaggca acgttattca caataaaaat
    16741 acgattctca cccatgcagt gaacaacgct attttaaaaa cggataacaa cggcattcag
    16801 attaataaat caaagaacag taacaaaatt gatccaatag ctgcattaat caatgcctat
    16861 gtgtttgcaa tggattactt caccacaacg gaaggagcga aagcagacaa tgaattttat
    16921 acaagtgaag aattttcttt ctaattacat tcataccgtt cttttacttc ttggattggt
    16981 gtgtgtgttg gttgctatca ccttactaac aaatgtctac tatggcttgt tagcgctggg
    17041 catagtgctt attggggtag cggtcatgct aaatacagaa cagaagggag gttaaaagat
    17101 ggcatttttt aaagcgagac aaaatacaac gggagatcct ttcctacaac atgtggtatc
    17161 aatccaatcg gatgattaca ccaccagctt tacaagcgtt cgtgcattaa gaaatagtga
    17221 tgtgtttgca gccgttcgaa ttattgctag tgatattgct tcaagtccga ttcaattggt
    17281 caaaaacaat atgccacaag ctgataatga actggtgaag ttactaaacg agaaacctaa
    17341 ttcagaaatg gatggttggc atttaaagtt tgctttggcg gtcaatatgt tgctaaacgg
    17401 taatagcttt gcagagatta aacgcaacgg tgaaaaggta gaagaaattc acttattacc
    17461 taactcaagt gtaacggtta ctcaattaga taatggtacg ttgtcttatc agattggtga
    17521 taaaaaaaga cgtgtgaagt ctagcgatat tttgcacttc aaatatttca cccaagatgg
    17581 tttaacagga ttaccaccac tttatgcgtt acgtgatgaa ctgaaaattc aacaagctgg
    17641 caaccgcaca ttgcacaatt tctttactcg tggtgtcagt gggtcaggta ttttgaaggt
    17701 tcataagtca gatttagacg gatcagcaaa gaacgcaatt cgtgaaaagt ttgaagaagc
    17761 caacggttca agtagtggag ataatgctct tagaacgatc attcttgatg aaacaatgga
    17821 ctacaaaaca ttagaaataa acaccgatgt tttgaagctt atcaattcca atgattggaa
    17881 cacaaaacaa attgccaagg cgtttggtgt accgattgag cgtttaggcg ttgaaaatga
    17941 acattctagc accgttcaaa gtaatctcca gtatatccaa agtacgctga ttcattactt
    18001 taatgtgttt gtgagtgaat tagatacgaa acttgaaacc aatatccgct ttaattccga
    18061 tcagttacta gagactgatc ctgaaacaaa agtaaaaaat gtattggatc aggtcaaagg
    18121 gtcacttctc acgattaatg aggggcggtc gaaaatgggg ctacccccca tggatggtgg
    18181 cgatcgttta ctagcaagtt tgaactttac gtatttagat acgttggaga aatatcaatt
    18241 aaaagaacag gaaggagtta caccagttga ataatgaaga agaaaaggaa aaacggctga
    18301 cagaagaagc tgagctaaca gccgattctc ccaaagtaga gaaagaaaat gaaaaacaac
    18361 caacagacgg caaaactatt tcaggctatg cgttgaaatt cgggcaaccg tcaaaagatt
    18421 taggcggctt tgtagaagtg attacacccg aagcattaaa agaggtggat ttatcaaatg
    18481 tgttcttatt atatggacac gattattcaa agccactagc tagtgtcaaa gcaggtacgt
    18541 tgaaattgaa tgtcgatgat acaggattac attttgaagc agagctaacc gacacgacct
    18601 attcaaatga tgtgtatgag aatatctcaa aaggggttgt tgattctatg tcctttgggt
    18661 tcgtgcttgg attagattca ttcgacaaaa aagaagatgg cacaattgaa cgatcaatag
    18721 ataaaatcaa agcacttaat gaaattagtg tcgtgaccgt tcccgcttat gattcatcaa
    18781 atgtccaagt aaataagcgt tcttacgaat cgtttatgag taacaaccaa gcaaaacaaa
    18841 caaacaatag cttagaatcc acttctaaag cacaaaagga gagtaataac atggaaaaaa
    18901 ctttaatcga taacgaaaaa acagaattgc gtgggtatga agaatatatc cgttcacaag
    18961 gcgaagtgcg tgatggagtc actactgtaa atgcagcggc agttgttccc gaagaagtaa
    19021 tcggtgaagt ctttgatttg aaacgttcaa attataactt agctcaatat gcaacagtaa
    19081 aaacagtatc aaatggacaa ggtaaatatc cagtagcaac taaccaacaa gcagtgttag
    19141 caacaaaagc tgaacttgct gaaattggtg atattgacgc tgaaatgttt acttcagttg
    19201 attataaagt agaaactcgt gctggtaaga ttgccttatc aaatgaggtt gtggaagatt
    19261 cagcagtgaa tattgtacaa gaggtcaaag atcagttagc gaaattggta gaaaacactg
    19321 acaataagca tatcatggat ttattaaaaa cattcactaa gaaaacggct gctacgttgg
    19381 acgatttgaa acaactatac aatgtggcat tagacccagc attaaataaa atggtaattc
    19441 taaaccaaag cggatattac cacctagata cattgaaaga ttcagatgga cgttacattt
    19501 tacaacccga tgtgacagca cctagtggta aatcattatt cggtatgcca gtagtattga
    19561 ttgcagatac attgtttgcc aatcctaaag ctggaacatt ccctatgatt atgggggata
    19621 ttgcacaatc tatctttgtt gctcgtagaa atcaagtaac gactcaatgg gaaaaattcg
    19681 attactactc acaaggactt gcagtgatcg ttcgcaacga ttataagaaa attgatcaaa
    19741 atgcttcagt gtatattgag tttacgccag ttgtaacacc aaaagtatag aaaaatattg
    19801 ggcggcggtt tatcccactg cctttttttc attaggagag ataaaatggt aattttggat
    19861 agcataaaaa aaagtatgcg gatcgatcac actattgatg atgactttat tcaacaattg
    19921 attgatacag caggcgaata tataaaaagt gctattgata gtagcgcaac ggataaagat
    19981 atggataatt atcagcaatt tgatttggcg gtgtcattac ttactcaaca ttggtatttg
    20041 aaccgtcaag aagccagcag cgaacggata ccagtaacgg tacaagcatt agtacaacaa
    20101 atgagaggtg cttattatgc caatcattaa gaatgtaaat gaattgactg agagagtcaa
    20161 gtttaaaaag acaaaacggg taaaagatga agatgggcag atggtggata gtgaagaaac
    20221 tgtatttgaa tgttgggcta atgtgcgttc acaaatgcta aaagacgtgc ttgctagtgt
    20281 aggtaccatt cttgaaggaa cgttgacgtt tattattcga tacgatcaag attatgaact
    20341 aacaaacgat atgaaagtag cttggaaaaa caagagttat aaaattattt caatcaatga
    20401 aggaacggcg tttaaagatt atacaacgat catagctaaa cagatttctt agaatgatta
    20461 cacttgtaaa cgttgtgggt atttagtata attaaggtag taaatgaagg gttagctgcc
    20521 tgaattttgc aagacttagc tagtcgaaat ctattgttgg actgaaaatt gtaggtgtgg
    20581 ttgcaaacat gctggactaa gaaagatgat agatgatgaa cgttcaattt taatacaggc
    20641 atgctaggac tgattggatg gttttggcaa gctgattgtg tagaaatcaa agaacacgtc
    20701 tgttgtggac gtgttttttt tgttataatt caaaagaggt gatttaatgg acaaagcaga
    20761 gtttacaaat ttttattgga agtattatct acatttagaa aatgagttta ttaataccac
    20821 agattttgtg atgctcgatg agaataattt caaaacattc tctattgaat atcaaaaact
    20881 actactagcg attggttcgg agtgtgagat tattttcaaa gaactttgcg gatttgatag
    20941 cagtagcaat aaaagcataa ctgattttaa agctgttatt aaatctagcg aattaatgtg
    21001 tttggataat ggtgttaggg ttatgaattc tttaacattg acttcactaa aaccttttgg
    21061 agatgggtgg cctgaagata caccagattg gtggaaaata tataacagag taaaacatgg
    21121 tcgttcttca aattataagg aagcaaattt ggaaaatgtg ttgtatgctt tagcaagtct
    21181 atatttatta gaggaatatt tgcataagaa agtaatacta gaaggtgaag tagactttat
    21241 aagtccagag tcaaatctat ttacgttatc ttgggaaaga aaacattcgt cgatgcaaaa
    21301 agtgacgttt gaaaaagtgg attcaaatta tgtagctcca ctcattgatt ttaaagaaca
    21361 agaagaaatt taaaaacccc cacggcttat tgaagttttt gtatgccata ttgtatgcca
    21421 tttggtttaa atcgcttgaa aaatagtgat agataactct aattcattta gtcataaacg
    21481 ctgttttgat aacctttata gttctttgat atgggtttct cattcatggc ggaagaagaa
    21541 gaatagaaca aaagaatgaa ttaataagat ataaaaaaag ggattgtgac aaacgtcttg
    21601 tcacaatccc tttttccaaa taaacgatgt tcaatcagct gcccctcggc attcgctcga
    21661 aaaacggcga aatatgaaga actattttgc catttgtttc tctaataatc aggtcaaaac
    21721 gttgacttca cagtctcttc cttaaacggt gttctcctgc caactcctcg gtcacaagtc
    21781 acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga ctcggagctg
    21841 aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata
    21901 agttcaacca aacgaaaatt atgaggagct atttttgttt ggctgttctt attacttgat
    21961 gcaggacagc tttttcacaa cctcttttcg tttagcacta aatttcctta aaaagtagaa
    22021 aagctttcga agcctcgttt gtttctttta ctgtataata aaagaaaaga gtaacaatcg
    22081 ctaaaagata taaacaagag aaaggaaata caagatgatt ggtctgattt attttgtggt
    22141 gatcgttctg gcgaacacag ttggcgctat ttccgggatg ggtggaggcg tgttgatcaa
    22201 accgattctt gatcttatcg gcgcccattc tgttgcaggg atttcttttt actcaacagt
    22261 tgcggtattc acgatgtcca ttgtttcgac tgtgcgacaa gtatcaagcg ggaaatcact
    22321 gaattggcag attgtcggtt gggtatctag tggagctgtt gttggaggag tagtcggcaa
    22381 cattgtattc gaagtttttt tacagttatt tgaaaacgag aagcatgtgc agctgatcca
    22441 aatttttttg acggtattga ccttggtttt tgcgttcttc tacaccaagc atcatcagcc
    22501 aaaatttcat ttgacgtcat ggacttggta ccttatatgt gggggggttc tgggcttttt
    22561 agctagtttt ttaggtattg gtggtggacc agtcaatgtt tctttgttga tgctgatgtt
    22621 tgcattgcca ataaaagaag caacactata ttctctatct acgattttct tttctcaact
    22681 ggcaaaattg gtaacgattg ctttaacaag tagctttatg cgctttgatt tatctatgtt
    22741 gttttatgtt atccccgctg ctgttattgg aggtttgtgg ggagcaagat ttagcagggt
    22801 tttgtctcca aaaaaagtga cctttatttt tcaagtaatt gtcctcgtgg ttcttttgat
    22861 caatctttac aatgctttcg tcatcttaca aacattagga gaatctagac taacgcaagt
    22921 caaaatcact gaaacatatt gttctaaatg aaaaaaggag tatactgtga taaaaaaatt
    22981 catttaaaaa aataaaaatt ggctggtgat ttggatgttg cgggaaatgc gtttaggaga
    23041 tgcagaagca ctacgagaaa tcaatgcgga acaattagga tatgatgtac cattaaccgt
    23101 tacggtgaga caaatgaaga aactgttgaa ggagccagaa aaaaattttt tcttggtcta
    23161 tgaagatcct acttctaaaa aagcagctgg ctatgtacaa gcggagtcct atgaatcgat
    23221 tttcagcgat ccgatgttca atattatggc acttgctgtg tctaaacatg ctgaaaaaag
    23281 aggaatcgga aaggccttga tgactggcgt agaagaagcg aaaagacgag gaatatcagc
    23341 aataagactt aattctgcag aatacagagt agaagcacac cgattttatg aacgcatcgg
    23401 ttatcattcc gataaaatgc aaaaaagatt tttgaaaatc ctgtaaattt ttatcaaaaa
    23461 atacctggtc atcaagccga tacttgatga tcaggtattt tttttgacta agaaagtaat
    23521 tgcatcaaga aacctgagat gatcaaaacg atggcaccac caagccgatt gcccatttga
    23581 gcaaaagcaa taagttccat ccgattagct gcagataaga cagctacatt tcctgtacca
    23641 cccatcgagt tgttgcataa gccagcagta atagttgatt cgattggata taaaccaaac
    23701 aaacgaccga caaatcctga aactactgaa atcgcgataa cacttgtcaa acataagaca
    23761 acaaattgcc atgtcaaagc agaagccaat acatttagat tcaataacgc aataccgata
    23821 ccggcaagaa cagctgctgt caagtttttg acaatcaagt tgttgaacat gatagctgca
    23881 tcttcgtaat attctggtaa taggtttgcg attttacaga taacaacgat aatgatcatg
    23941 aatgcgtaag catggacttt aggaacaaaa tagttacaga tttgtcctaa gatgaagaaa
    24001 gtcaatgata ccatcaaacc gacacctaat tgtggaatgg aaggttttac atttctaggt
    24061 tttccatcgc caaggtcgcc tttttccatt aatttaccat taccgttgaa ttttggcatt
    24121 gattgtcccg cacgagcaac taacgctgca ccaatgatag ctaggacatt tgtcattgct
    24181 gtcgcaggaa tcaggcggga aatgatagaa gaagcgtccg tacctaaaac atttgcataa
    24241 atcgttgata aaggtacagc tccggctcca acaccaccag ccattgcggg caatgagata
    24301 tataaaacgg aatttgcaaa accatttccg ataagcatac ctacgagtcc tacggcaaaa
    24361 aaggagacga ccattgaaat aagtgcaacg ggaataaaac gaacagaagc tttcatcaat
    24421 agattacggt tcataccaag gatgctacca gtaatcaagg cagcaatata aaagtctaaa
    24481 aagcccatat tgctgacaaa gttttctgtg gattttacta catactcagg taagatccca
    24541 aatgtagcaa tcgcggcaga ggcaaagatc gcaaaaacgg agccaccacc taaataactt
    24601 ttgacgatag ggatttttgt tccgataaaa tgaaataaat taccgaaaat gaccaaaaca
    24661 gcgattgggc caatcatatt cgtcggtaat tttcctaaag ccattgcaat gatcaggaca
    24721 aggaccatga tcaaataaag gggcaaactt acgccggtaa tttttgttga ccaaaaccct
    24781 tcttttttct tttcctcaac tgcagttgcg gttgttgttt tttgtgccat ttttacagat
    24841 cctttcttat tctgtatcgt atgaaaaaag ccaaagagaa gcggcaaaag agcagtagtt
    24901 ggctggctgc tgttttgccg cagtcgcttt tttcttttta gacgtcatac aaagtttctg
    24961 tatgttcttt tactataaca atcttaatct tctaaagatt tatattcagg aacccattta
    25021 gcagattcaa cagcttcttt gatatccgtg atttcttcac gattcaattt ttgtgcaaca
    25081 actgattttg cgacagtttc agcaatcgtt tgagagaatt cagtgatttt tgctactgga
    25141 ggcaagatag ctgctcctgg ttttgttaca tcaacgattc cgcctaatgc tcgactagct
    25201 tgagaaatga tttcagcatt tactcgtgta gctgttgaag caatcaaacc aagccctaaa
    25261 cctgggtaca tcaacgcatt atttgcttga ccgatttggt aagttacacc gttatattcc
    25321 acatcagcag cagggatacc tgttccaact aaagcttttc cgtccgtcca ttcaatcaaa
    25381 tcttttgctt ttgcttctgc caattttgtt ggattagaca aaggcatgat gattggacgt
    25441 ggggtatgag ccgccatttc tttcacgatt tcttcagtga atgctccagg ttgggtagaa
    25501 gtaccgatca tgatcgttgg atggattgct tttacaactg cttctaagtt tgtcaattca
    25561 tcagcattag tgaattctga gcgtttacga gcgaatggga tttgaccagg agtcaatcct
    25621 tttgtatctt caaacaataa accctgttta tctacttgat agaaatgttg gcgtgcttct
    25681 tcttcaggta caccttgacg gatcatttca tctaaaagga tgtttgcgat acctacacca
    25741 gcagtaccag caccaaatgt caagatcgtt tgatctttca attgctcacc tgatatattc
    25801 aatccaccta atacaccagc tagtacaacg attcctgtgc cttggatatc atcattgaaa
    25861 gtagtgattt tatcttcgta tttttctaag atcgttgcag catttcctcg tccaaaatct
    25921 tcccaatgta ataacaattc tgggaataat tcagtagctg attgaacgaa ttgatcaatg
    25981 aattcgtagt aagtttcgcc ttctacacga gcatgacgat tccctaagta taatggatta
    26041 ttcaataatt cttcgttatt agtaccagcg tcgattgata caggcaagac ttgtgcagga
    26101 ttgataccag ccgcagcagt gtagaccatc aatttaccga tagcgatgtc aacaccgttc
    26161 acaccccagt cacccatacc taagattcct tctgcatctg tcacaacgat caaacgaatg
    26221 tcgcgtccat ctgctgcgtt tttcaaactt gctttgatat tttctggttc gtcgattgaa
    26281 agaaatgcag catcttgtgg tttcaagaaa atttcgttgt attgttcgat tgaatccgca
    26341 acgacaggat cataaacgac aggcatgaat tcaactaaat gttcgcccat caatttataa
    26401 aacaatgtcc ggttcgtatt gaatagattc attaagaaga tacgtttttc caaatcggtc
    26461 tgtttgctta agtattgacc gtaagcttgg acagattgtt gttccaatgt ttggacagta
    26521 cttggaagca taccagtgat tccgtatttt gcccgttctt cttttgtaaa agctgttcct
    26581 ttatttagaa aaggattatt taataagttt agccctgtaa gcatgaatga ttcctcctaa
    26641 gttttaatac aaacgaagta taggagtcag atgaggatat agtcaaaaag agcaggtata
    26701 ataaatttta tttatatgag taatagagtc aaaagaaatc aaaatagcgg tatttgttct
    26761 ttttatatca acaatccttg cttgattact atgcgtatct cttatatgct aaagaaagaa
    26821 aagtagaaga ataaaggata tgttagatag ataaatagaa tctctgaaga ataaatcacg
    26881 tgatcgtgac gataaaatac acttacgtca ggtgataaaa aatacataag tagaaaacta
    26941 agaggaggaa cagaaaatga aagaagttca attgatcaga aaaagcgaac tatcagaagg
    27001 tggttgcaat gcatgcggcg tagtagaagc aacaagctat acattaaagc ttggtgccaa
    27061 caaagcgata atctctgagt tgacagtagg cggtcttgtg gacagtctgg cgcttgctga
    27121 aggttttatt ggagaagata tttatgagat gttttcagaa atccgtcagt taaaaaaagg
    27181 ggaaaactgc atagaggttc atcatgagag cccgaatgtg agattcaaac gtggtgataa
    27241 tgagatgatc ttcaataatc atgtgtccga tcacaccgag ctatatggaa tcgtgaatca
    27301 aatactaact gagctttttg gattgggacc ttatgcgttc aaggaagaaa acggtaatcc
    27361 aaaattaaac gaagaatggc aagaaacgat tgaaatacaa agaaacaacc cgcacttatt
    27421 tcaataggca tccacagtat ttcgtaaaag ggactttgac acgacttttg tcaaagtccc
    27481 atcgtttttt atctatttgt gttctaaaca tttctttcag agtagtaaca actggaatga
    27541 ctgaaatgta aacagattat ttgcctgtat caatacggaa gagtaatata aagtgtgtaa
    27601 acgctatcat attattcatt caatttttta tagagggaag gcattgaaat ggaaaaaacg
    27661 tatcaagcag gtacaaatga aggaatagtg gattttatca atatggaaga tttagaaata
    27721 gccgcttcac aagtgattcc agctggtggt tatggttaca tcagcagtgg tgctggcgac
    27781 ttatttactt atcaagaaaa tgaacgggca ttcaatcatc agctgattat tcctcatgta
    27841 ttaaaagatg tagaattacc cgatacaact acacattttg acaaagaaac gctgactgca
    27901 ccaatcatca tggcaccagt tgctgctcat ggcttagctc atgtgaaggc ggagaaagct
    27961 tctgcaaaag gtgtagcaga ttttgggacg atctatacag caagttctta cgcttcttgt
    28021 acattagaag agataagaga agcaggcggg gagaaagcac cgcaatggtt tcaattttat
    28081 atgagcaaag atgatgggat caatttggat attcttgaag tggccaagcg taatggagca
    28141 aaagcaatcg tattgacagc ggatgcaaca gttggcggaa accgtgaaac agaccgcaga
    28201 aatgggttta ccttcccact gccaatgccg atcgttcagg cttatcaatc aggagtaggc
    28261 caaacgatgg atgctgtgta caaatcttcc aaacagaagc ttagtccaaa agatgtagaa
    28321 tttattgctg ctcattctga tttgccagtc tatgtgaaag gtgtccagtc agaagaagat
    28381 gtctatcgtt cattagaatc tggtgctggc ggaatctggg tatctaatca tggtggccgt
    28441 caactagatg gtggtccagc agcgtttgat tcgttgcaat atgtggcaga agcagtagat
    28501 aaacgtgtac caatcgtgtt tgacagcggt gtacgacgcg gacagcatgt tttcaaagca
    28561 atcgcatcag gtgcagatct tgttgctatt ggcagacccg ttatttatgg attgtcttta
    28621 ggaggaagta caggagtcca tcaagtgttt gatttcttta aaacagaatt ggaaatggtc
    28681 atgcaattag ctggaacaca aacagtagaa gatatcaaaa aaataaaatt gcgtgagaat
    28741 cgttttattt aagaaacaaa tgacgaaaaa aataggagtg cggcagatga tgccgtactc
    28801 ctatttttat agttcaagga tgtcactaat agaataattt gaaacgaaaa aataaaattg
    28861 aaatattttt tcatacttaa taaaaaagga agaatattca tgtaaacgat tttattgttt
    28921 ttttctcttt tatacaaaaa gggtcataat ttcaagtaag cggatgaatt catgaggatt
    28981 ttttgcatac tgtctctact atacttaggt cactaagaaa aaggagtgat ttattatgaa
    29041 aaagaacatg attgggacat tattactttc aacacttctt ctaggcggct tggcaacacc
    29101 agcttttgct gagggtcaag cgacttcaaa aggagatatt acctttactg aaccaacaaa
    29161 taccgtggag cctcttaacc ctactgatcc ttcaaaacca gtagagccag cagatccgga
    29221 aaatccagca actggccaga caggtccttt aacactggat gtagttcccg aattgccatt
    29281 tggtacacac gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt caaaaaacga
    29341 tacaccttat ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc aagcacaagg
    29401 gtggaacgtg acagtttcgg tttcagattt tgtaaacggt tcacaagttt tgcaaggcgc
    29461 tgaattagat tttggaacat ccactgttaa aagtacgtca gataacgaat ccactggacc
    29521 aactagtcaa actgtaactg ggttgagtaa agcaagtgca gcgacaccaa tatttacagc
    29581 agcaaaagat caaggtctgg gaacatggct ttctgtttat gatccagcaa atatcacact
    29641 gaaagtgccg aaagcagcag ctggaacatt cacggctgat ttgacttgga acctcgtggc
    29701 tggcccagta gcatagagta tgaaataaat tcaacaaact gcggtgaaaa tactataggt
    29761 gaaggatttt ttaccgcagt ttttgttgaa aggatgtggt ggacaatgaa tcaaagtaaa
    29821 cgactgtttg tttcgttttt ttctatttta agtctatttt tcatttttcc gtcaatcagc
    29881 caagcaaagg attcagcagg agactttgga atcaaaccgg tatttccaga gaaccaaata
    29941 gataaagcaa tcggttattt tgatttatta gtggcaccgg aacaaaatca gacactagaa
    30001 gtaattatca gcaattcttc agatgaagaa cgaacatttg aggtctctgt caatccggcg
    30061 gtaacgagtg atggtggaac gatcgattat agtcaaaaaa acccaacgtt agatgagaca
    30121 ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa tgtatcagca
    30181 catgcagaaa caactgtacc tatagaagtg aaaatcccag caaaaagctt taagggacgt
    30241 gtattagcgg gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa tgctacagaa
    30301 ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag tcgttttaca agaaagtcaa
    30361 gagacgatag aaccggatct gaaactttta tcaggagatt tggacgaggt caacgcaaag
    30421 ccaactgttc agcttcgttt ccaaaatcct cagcctagga ttatcagcaa tttgatattt
    30481 acgagcaaga tcttttatga aaatcagctt tatattgaaa atacgtctaa tgcattttta
    30541 gttgcaccaa attcgaattt tcacttgaat cttgatttag ctggtgataa agcaaaagcc
    30601 ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg gcgatttaca
    30661 caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc cgtttttgca
    30721 gtacaagaac aatctttccc atggctttat gcaggattgg ttgctgcagt tctagtgtta
    30781 tggttgattt tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga aacaacatga
    30841 tgaggtgatt tcgttgaaaa catcgatctt attgggaggg attattgcaa gtttgctgtt
    30901 cttcagctgg caagagccag tccaagcaga taccaaaagt tcaggtacga ttacatttta
    30961 tgatctaagt ccaattccgc atccaagtgg ttcagtcaat ggcgcatcaa aacagcagac
    31021 acagctaccg atttataaag aatctaatca acatggcgta ttcccggaga cgaatgaaca
    31081 aaagatccgt tttctttatt tgatcattgg tatagaatta tggcttcttg cgtggttgtt
    31141 gttatggaga agaaaggaaa aggaggatga acatgttgct acaaaataaa ttttcatttt
    31201 ttcgctcctt cttttttact tcgctttttg ttttaagtat atttatcttt tcaccttcgg
    31261 cagaagctgt tggagcaacg cctcctgcca tttcttatga tccatacacg tatgaaaata
    31321 tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc caactaactg
    31381 gggctaacca attgcctaaa ttgactgcta cttatttgaa cgatggttca aatgatcctt
    31441 ggaaaggtgg cacggtccgt tttcattggg aaagccatgt gaaactaaca aatgtacttg
    31501 atttggtgaa tatcactgca caaggatttg catttgtggc gatcatcaat ttgccggaac
    31561 atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa caacgcctac ttgtatattg
    31621 ccggtgcccc catcaaggtt actagcggaa gtttattatc agcaggaagc cataccttgc
    31681 gttttgggat ggggtcgtgg gatacaaata ctctattgac agatattatt gctattatta
    31741 cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt tgatgttaca gctaatgtcg
    31801 cgaagatgac agaaaacgga gataaatatg atacgagtcc aaacaaaatt ttgaccaagg
    31861 gtaaattaca accttctatg tctaaaaagg ccgttttttc tgttgatttt tatgattcga
    31921 ataatattat ccaagatcgt atgttcaatt cgattttagc tccagctgca ggtagatttt
    31981 atcctaaatt aactggcgga aacttgaatc caggagcata tgcattttct gcaagtgcag
    32041 agataaatac gtggaatagt tacatctcac caagtgattc aacaggaaaa tataatacat
    32101 ttgctgatac cacagaatac gtggatggaa ataaccagaa ccttccaggc tctaccaata
    32161 gtcggacatt gacgatgggg ctgactgaag gacgttttga tcagttgcca tttagtcgct
    32221 ttgatcgcgt agtcaactat tttacaggaa aaaacgttac acaaggagca atactgactc
    32281 atactccagc aagcacaagt ggattgaatc agacagccaa tatcgtgtac agtgggaaag
    32341 atgcgcaagg cggattgctt tctcctgttg ctttaaaggt attggagcag tacaagctag
    32401 acggaacggt tttaccaaca aacttaacag ccgatagtca gtggggaaag ctcctttctt
    32461 ctgcatcgta ccaagtcacg gccaattggt cagcaactga actaaaaaag ggttcgatcc
    32521 attacaggct ttattcgaaa ggaacccaaa aattagcgac aggatggaca gatcaacttt
    32581 tccaaacgat ccaatcaaat aacgggggtg taaatacagc tacttctaca ctacctgcat
    32641 tggcttcagg gcaatattat tttgattatc gcttggtgga cgatgtactt actaacatgt
    32701 atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg aatcaccact cttcctcaaa
    32761 taacagtggc taattttcca tctgtgagtg cagatagtca gctaaaaaac cttagcagac
    32821 aacctgagac aggagatccc ctgtctgctc tatcaggaga tacgattcaa gaaaatgtga
    32881 cattcacgtt aacaaaatct ggggatgcga taaccgacaa aaaaatcacg atctcacttc
    32941 ctgaaaatac tacttatatc aatggttcct taaaattaaa tggtacaact atcgcagata
    33001 ccggcattca acaaggagtt tctgtcccag cagatctatt gagcaaaata ggagatacca
    33061 ttcatcttac ttacaactat caattaaata ctgtagatac ttctttacaa tccgtcagca
    33121 tactgacaaa ggcagctgta ctttccagca atattacatt agcagatggt gctaagcttc
    33181 ctaacccagt tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa gaattaacat
    33241 tagttaatgt accagatgat tttacatttg gtaacgatct ccccaaaccg ttgaagacaa
    33301 gttattatga atcaaaaggc gatttttctt ttgatgtgcg ggatactcgg cttccaagca
    33361 cgtctccttg gcaactgaca ggtacattga cttctttatt taagaatgat caaggtcaag
    33421 aattaagcgg aacaaaatta tacttcaatc attctggatc aaagcaattg attcagcaag
    33481 gtcaaaacac gttgatttat gagagtgatg gaacagctaa aggagaggtt ctcgttgact
    33541 ttcccgatac cgatggttta ctcttagagg taaatagcag tacgaatgct caacctggcg
    33601 caacgtatca aggcatggtc acatggaaac tgacagctgg gccaaccagc taagtgatta
    33661 gaatttgtat ggatctgtga taactgtcac agatcctctt tttcaaggat gagaaggaaa
    33721 cttctgctgt taaaatcaga aatgcttgaa ggagtggact tggaactttt cagtttgctt
    33781 gaaaaaaatg aacaacggca aatcaaactt ttccaacttt tattagagga aaaaggagag
    33841 gtttccgtta ataacgtact tacccgctta tcagttgatc gggctacctt gaaagaagat
    33901 ctgttttatt tacaagctaa cttggaaaat atcaaacaat tggaaattgt tatccaagaa
    33961 gggaatattt tattaatcag acatggaaat atagcagcag tcgatgttta ttatcattgt
    34021 tacttgcgct attctacaaa atatcagata cttctttatc tgttggaaaa tggctcattt
    34081 gatcggcaga aacttgtcat ggaacttaat atcagttcgg ctactttgtc tcgcagagtg
    34141 aaggaattaa atgattcttt aaatgagttt catctgaaaa taaaaagcgg gcggctgacc
    34201 ggtccagaaa gtcaaattcg ttatttttat ttccaattat tttggtttgg acgtccctat
    34261 cttgaaaaca aaaaagaata cagtgattta tcaggaaaca cttttttaga tctgctgcaa
    34321 aaagagttta gttttgctat tacagaagaa ggacgattga aatttgaaat atggctttcc
    34381 attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac cagattatag caatcttcct
    34441 gttggattgt ttcaaacgac acaattttta ttggacctcc atacattttt gacccgttac
    34501 ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt tggtttttta tcttttcatg
    34561 atcagtaact ttacattaga tacacataac tcgcatgtcc aggagttttt gataagcgaa
    34621 aaatacaaag acgacgtagt agctcaattg aatcgaacgt ttaaacaatc gatagaagaa
    34681 tatttctctt tttatgtttt tccagaagag atttcgcaaa agattgcgtt gacagtgatg
    34741 caaaatcacg ctcattttgc ctattttaga ggatggatga atatttttgg ccaagaagga
    34801 ttaaaacagc ggttaactga agtagaagat aaacaattcc tggttttatg tgaaagattg
    34861 gtcagacaaa catttgctat tgttgatttt actgaagtag acgaggaaaa tgcccgcttt
    34921 gaattatttg ctcgctatgc atctatttta cggacgctga tcaagcagtt agaattacgt
    34981 ctgcaaattg cttatgatct tccttatgaa aggctaatgg cggatatcgt gaaagaaatc
    35041 atccaggaac aattgctgaa tacgatcaaa tatgatttag tcgaatacga gaaagaaaag
    35101 cagtatgata tcattttgac tagccaattg aaagagtacc cgtcacagaa gtccgctaaa
    35161 gtttttgtat tcactagcaa cgaatataat tatgatttcc cctatttgaa ccaattttta
    35221 aaagaatgtt atttagaaaa attgaattta aggatgaata aaacatgaaa atcgtgatca
    35281 ttggtgcttc ccatgcaggc atcacagctg ccctaaatct aagaaaactt caaccgaaag
    35341 cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt tcaaatggaa
    35401 tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat aacatgcgga
    35461 ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa cttgatccag
    35521 acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata acctatgata
    35581 aattaatcgt agcgacaggt tcctctcctg tcaccttgta taaacaaatt gaagctgaaa
    35641 atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct ttaaaagaag
    35701 caaaagaagt agtgatcttc ggtgctggtt acattggatt ggaattagca gatgctttga
    35761 gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta tcaagatatt
    35821 tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa ataaattttt
    35881 atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta tccgtacaac
    35941 ttctgtctca aacaataaag gcagatatgg tcattttttc tgcccaaact cgtcctaata
    36001 cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata gtcaatgaat
    36061 atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca gtcagttttg
    36121 acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat atggcaagag
    36181 cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa aagataacag
    36241 cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat gaagcgccat
    36301 tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca caatacgacg
    36361 aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta gaaataattg
    36421 gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt gcggatatcg
    36481 tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt ctgtctgaat
    36541 acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct aagagagcaa
    36601 atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt gtgtggggat
    36661 ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc ggaagaattt
    36721 tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat aaaatgatta
    36781 tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt caaagttacg
    36841 ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt ttttgactgg
    36901 gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg aggcagattc
    36961 gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta ctaagaagat
    37021 agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc caaacacctg gagtcgtgac
    37081 tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta tcgataaaaa
    37141 tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg atgaggcagt
    37201 aggcgatatt agtttgaaag aagaatatgg tctagccata ggagatacag ttaagttatc
    37261 tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat tcaatgtctc
    37321 accagttctc tatacaacca tcaatgctta ccaagaaatc cgcttcgaaa aagaagatac
    37381 aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta atttacctga
    37441 agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat ataatgcgca
    37501 agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta ttgtgatcgg
    37561 gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa tgaaagcaca
    37621 agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca ttttatcatt
    37681 tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc tacctgatgc
    37741 ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga tgttagtagt
    37801 agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc cgttgaaggc
    37861 aattggataa gaaggaggaa gaacatgaaa gcaatagaat ttaaagaagt agaaaaaagt
    37921 ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag tatcgataaa
    37981 ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt tttgactttg
    38041 gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca agaattcagt
    38101 aatcaaaaag aaagcgaacg cgttaaacta cgttttaaag aaattggctt tattttgcaa
    38161 gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt agataagatt
    38221 aaacatgaac atcgtcaaaa agaagcaaac gaattatttg agcagcttgg cgtggataag
    38281 ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt cgcaatcgct
    38341 cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc tagtttggat
    38401 tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga caaaaataaa
    38461 gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag aatatttatg
    38521 atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa aaatggtcca
    38581 tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact tgtttatgtc
    38641 caagggatat ctcggcactt caacaagaga aatcgcacaa aaagttggga tcacccagcc
    38701 caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt tagaaaacca
    38761 cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa cgggatttca
    38821 agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg atttgtttct
    38881 gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc ttttctttct
    38941 atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc aatctgtgct
    39001 aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt tagcacctta
    39061 catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat tgatcgattt
    39121 atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa aatagttagg
    39181 tgaagaagca agatatgacg aagatcgtta tatcttgctt ctttatatag gtagtttttc
    39241 ataaagacaa atttgttatt ctctcatgat tatcagggta tatttttatt tattatgtcg
    39301 gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta gaatgaagga
    39361 aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc aggatatgcc
    39421 aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat cttttacagc
    39481 cccttttttg cttacttaag ctgattttaa gttttggccc ttagtctgga tctacgaggt
    39541 gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt atcattatta
    39601 tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg gtcagcagta
    39661 cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa gagacattaa
    39721 tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact accccacttg
    39781 caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat gcttatcaga
    39841 tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc aaagtcacaa
    39901 agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta gatcctgggc
    39961 atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa aaagatataa
    40021 cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca acggtttatt
    40081 tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac agtgaagaaa
    40141 aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg aatgaagcaa
    40201 ctggaatcac gacatattat tactatggac aagaggaaac attagcacag acaattgctg
    40261 actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac tatcaagttc
    40321 ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat aatgatagtg
    40381 atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt ttgacacaag
    40441 cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag ctcctcatat
    40501 ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt tgaacaccgt ttattttttt
    40561 agtagcttca aagctttttc tgtatttgca aagtgaagtt tggcatattt ttcaagcata
    40621 ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct
    40681 gatggaaggg tgattccagc ttcagctgat gctttgtgta actcttccaa aaagctggca
    40741 cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag
    40801 tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc
    40861 ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc
    40921 aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg
    40981 atctcattgt atttttctgg agataagaca agcagtttat aggggatggt tttttttaag
    41041 atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt
    41101 ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggaccg
    41161 aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt
    41221 ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta
    41281 tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta
    41341 atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa
    41401 taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg
    41461 ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc
    41521 gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt
    41581 atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa
    41641 ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcgg
    41701 tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg
    41761 taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata
    41821 atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag
    41881 aaagaatctt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta
    41941 cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat
    42001 tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt
    42061 taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca
    42121 aaataagaaa aatttaaaaa aagttagaag agaaaggaac gtagcatgct gagtttgctg
    42181 attttatttt tattgctaat tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa
    42241 atcatattcg cgattggata tgtattgtca tttattgctg cccagcattt ttacaaacca
    42301 ttagcaagtc atttggaatt atacattcca tacccagctg tcacgccaac atcaaagtta
    42361 gcattttttg atcaggtatt tgcctttcac ctagatgaag ctttttatgc aggaactgct
    42421 tttctgatta ttttgctaat tggctggctg cttacccgat ttgtgggggt ttttgtccat
    42481 ggattgacgt atgtgccgat cttgagacag gtagactgga ttgcaggagg catcctgtca
    42541 ctaatcatgg cttacgttac tatatttttg attttgcagt tacttgcctt tgttccatta
    42601 gatgtggtcc aaaatcaatt cggcgagcat agtttcgctc gctttatcgt tgagagaacg
    42661 ccatttctta ccaacaagat gtatgagctt tgggtaacga atattttgaa ttaaggaaaa
    42721 tagtgctgga aaatatttcg ttttttgtac agagtagatt atttaagaag gctgcgtagc
    42781 gtatgtccgg cacagctctt ttttcatagt gaaagataag gtgataaaat gaataaccga
    42841 atccttgaaa cattggaatt tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact
    42901 gcacaaggaa aagaagaact ggcagaattg gtaccagtga gcgaggaaca aacgatcaca
    42961 aactggcttc aagaaactga ggatggattg aaagtgcaac gtctgcgagg cgggatccct
    43021 atacctaaac ttgaaaatat ccgtccccat atgaaacgga ttgagatcgg agcagacttg
    43081 aatggagtgg agcttgcaca agtttccaga gtattgtcta cgacaagcga attgaatcgt
    43141 ttcattgatg atctttatga cagtgaaatc gaatttgcac gtttgtatat gtgggcagat
    43201 cagttagtca cgatccctgt tttgagccgc cgtttaaagg aagcaatcga tgaagacgga
    43261 cgcgtgactg atgacgcttc tcctgagtta aagagcataa gacagaacat tcggcgaagt
    43321 gaacaagctg ttcgtgaaca gttagatggg atcgtccgtg ggaaaaatgc aaagtattta
    43381 agtgatgcga tcatcaccat gcgaaatgac cgctacgtta tcccagtcaa acaagaatac
    43441 cgcggtgtat ttggcggtgt agtccacgat caaagtgctt caggacagac tttatttatt
    43501 gaaccaaaac aagtggtgga tttgaataat cgtttgcgtc aataccaaat cgctgaacga
    43561 aatgagattc aacgtatttt aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa
    43621 atcatccaca atgcttatgt gattggtaaa atggatctga tgaatgcaaa agcacgattt
    43681 ggtaaagaag taaaagctat cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa
    43741 caagcaagac atccactgat cgatcaggaa aaagtagttt ctaatgacat cacaatcgga
    43801 aaagactatc aagccatcgt cattacagga cctaacacag gtgggaaaac gatcacgttg
    43861 aagactttag gtctgttaca actgatgggg caagcaggat taccgattcc tgcaggtgaa
    43921 gaaagtcaaa tcggtatttt tgaggaagta tttgcggata taggagacga acagtcgatt
    43981 gagcaaagtc tatcgacctt ttcttctcat atgacgaaca ctgtagatat tttatcaaaa
    44041 gtcaatgaaa aaagtctggt attatttgac gagctcggtg caggaacaga tccgcaagaa
    44101 ggtgctgctc tagcaatcgc aatcttagat gatcttggta aaaagtctgc ttatgtgatg
    44161 gcaacaacgc attatcctga attgaaagtt tatggatata atcgggcaaa tacgattaat
    44221 gccagcatgg agtttgatgt ggatacatta agtccaacgt atcgtttgct gattggcgtt
    44281 cctggacgaa gcaatgcgtt tgaaatctct agccgattag gtcttgatac agaagttatt
    44341 gatgaagcga agcagttgat gaatgatgag agccaagatt taaacgaaat gatcacagat
    44401 ttagagaatc gccggaaaat ggcagagacc gaatatctgg aaatgcgtca ttttgtttct
    44461 gaggcacaag aacttcacga tgatttgaaa gaagcttaca gctatttctt tgaagaacgt
    44521 gaaaaagaaa tggaaaaagc aaagaaaaaa gcaaatgaag tcgtgtcaga ggcggaagaa
    44581 aaagcagaaa aaatcattgc tgatattcgt aaaatgcaac agcagattgg gcaaggaaat
    44641 gtgaaagagc atcagttgat cgatgcaaag acccaattag caaatctaca tcaagaagaa
    44701 actttgaaga aaaataaagt attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga
    44761 gacgaagtgt tggttacgac ttacggccaa agagggacgc ttttacgtaa aaacgggaat
    44821 cagtggcaag tagaaatcgg tatattaaaa atgaatgtgt ccgaagacga gctgacgcct
    44881 gttgctcctc aaaaagagcc gacacaacga gttatccatg ctgtacgatc tgaatctagt
    44941 agtcacgtgc cgaatcaatt ggaccttcga ggaaaacgat acgaagaagc gctaagtgag
    45001 gtagatcaat atttggattc tgccattcta gctggttatc ctcaagtgac tattgtccac
    45061 ggaaaaggaa caggcgcact tcgcaaggga attactgatt atcttaaaaa ccatcggagc
    45121 gtcaaaagct ttgagtttgc accggcaaac caaggcggaa atggtgcgac aattgttaaa
    45181 tttaagtaaa gcttactaaa ggtaagcaga tggttcgcta tccaagtata gaaatgctat
    45241 actagtcata gattttaaaa atggaggtct ttgatatgtc acaagtaatt acagataaga
    45301 catttaatga agaaacagat aaaggacttg tattgattga tttttgggca acttggtgtg
    45361 gtccatgtcg tatgcaagca ccgattttgg atcagttgga acaagaatat gatgaagaag
    45421 aattcagaat tgctaaaatg gatgtggatg aaaatccaga aactcctcaa caattcggta
    45481 tcatgagcat tccaacatta atgttgaaaa aagatggtca agtagtagaa aaagctgttg
    45541 gtgttcattc aaaagaacaa ctacgtcaaa tgatcgatca atatttataa aaacttacag
    45601 caaaaataag ctgagggtgg gactgaatcc agttccgtcc tcggtttttt tgcggttgag
    45661 ttaaggttat tcaaattaat attttttgag tataattacg ttgtcaggct ggattgtttt
    45721 tttgacagac atgctataat aactttaatt gaatatgaag gggagtacca gatgaacgaa
    45781 cgaatcaaaa acaaattagc tttacttcct gatcagcccg gctgttactt gatgaaagat
    45841 aaaaatggaa cgatcattta tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca
    45901 tattttactg gtagccataa tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt
    45961 gaatacatcg ttacagaatc gaatatcgaa gcattgctct tagagatcaa tctgataaag
    46021 aaaaatgatc ctaaatacaa cattatgtta aaggatgata aaacctatcc tttccttaag
    46081 ataacgaatg aaaaatatcc ccgtttggtg attactcgta aggtactaaa agacaaagca
    46141 ctgtattttg gaccatatcc cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat
    46201 cgattatttc ctctgagaaa atgcaaacct agtcagacga aggagccttg tctttactat
    46261 catttgggac aatgtctgtg tccttattat ttcgacgtag atccgaaagt ctatacgaaa
    46321 atcgttgatg aagtgaaaaa ctttttaaac ggaggacacg aaaaaatcga gtcagagatc
    46381 catcagaaaa tggaaaaagc tgctgaaaac atggagtttg aaaaagctgc agaataccga
    46441 gaccagatcc gtgccattga gacaatcatg actcgccaaa aaatgacaaa tactgattta
    46501 ctggatcgag atgtatttgg ttatgcgatt gataaaggat ggatgtgtgt ccaagtgttt
    46561 ttcgtaagac aaggaaagct aattgaacga gatgtttcaa tgtttccttt ctataacgaa
    46621 gcagaagaag atttcttgac ttacctcggt cagttttatc aggaaaatga gcatttcatt
    46681 cctaaagaag tcttgattcc tgatacgatc gacaagccca gtgtagaagc tttgttagct
    46741 acgaaagtgc tgcaaccaaa acgaggagaa aagaaaaaat tagttgaatt ggcgagtaag
    46801 aatgcctcag ttgctttgaa tgaacggttt gatctgattg ccagaaaaca ggaacggacg
    46861 atcggagcag ttgaacgttt aggaaacgcg atgaatatcc cgaccccaat acgtatcgag
    46921 tcttttgata actctaatat catggggacg aatcctgtgg ctgcgatggt cgtattcatt
    46981 gatggaaaac cggcaagaaa agaatatcgg aaatataaaa taaaaacagt caacggaccg
    47041 gatgattatg catcaatgag agaagtcatc tatcgcagat attcgcgagt gattcgcgaa
    47101 gggttgcctt tacctgattt gatcttgatc gatggaggaa aaggacaagt agatgtggca
    47161 aaagatgtct tagaaaatca attaggaatc gatattccta ttgctggcat ggcaaaaaac
    47221 gataaacata aaacaaatga gttgctattc ggtacagatt tggctgtagt accgcttgag
    47281 cgaaattcac cagagttttt cttactccag cgaatccaag atgaagtcca tcgattcgca
    47341 atcactttcc atcggcagct acgcagtaaa aacagttttg cttcccgatt agatgaaatt
    47401 gaagggttag gaccaaaacg aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat
    47461 atcacagcag ccacgacaga agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa
    47521 aacgtatttg ataaattaca tcaggaatga ggaaagggca ctttacaaag aaagtaaagt
    47581 gtcctatcat tttatgtaca acaaaaagag cgtttacgtt ttgttggtaa ataataaatc
    47641 gcttacgtct ggtgtctaag gatatatgtg atttcttgaa gaaggagtga agaaatgtta
    47701 gaagtcaagg atcttgttaa aacttttggg aactacacag ctgtcgatca tgtctctttc
    47761 caaatccccg atggaaaaat catggggcta attggccaaa acggggctgg gaagacaacg
    47821 acttttcgtt tgatcttgga ttttttgaca caagaccaag gagaagtgtt atggaatggc
    47881 catcatcttg gggagaaaga ttatgatgtc atcggttatc ttccggagga acggggactt
    47941 tatccgaaag taacgattca agaccaactg ctttattttg cagaacttag agggaaatca
    48001 agaaaagaga ttgaaccaaa aatcgacttt tggatggaaa aatttcaggt aaaagggaaa
    48061 aagacggata aagtaaaatc tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc
    48121 acgttaatcc atgaacctaa attgatcatt ttggatgagc cattcagcgg gttagatccg
    48181 gtaaatgctg aattgctaaa agatggaata ttggaattga aaaaacatgg ttcatgtgtc
    48241 attttttcta gccacaatat ggataatgta gaaaaaatct gcgatcattt gatcatgttg
    48301 cgaaacggaa aaatggtttt gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt
    48361 acgaagttat tcttggaatc tggtttaagc caacaggaag tagaagaaat cgaaggcgtg
    48421 aaaaaagtga tccttcatga agacaatacg ttagagatta ccttagaaga tcctgaagct
    48481 gggaaagaaa tctttactcg tgcgactcag ttcggctaca ttcctatgtt taaccagcaa
    48541 ccgccaactt tggaagagat tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg
    48601 gattattacc aaagatgtct atttaaaaaa tgttaagtcg atttctttct tgatcatgat
    48661 attagtccct tttatcatgc tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa
    48721 tagtgatgtc aataaaatcg gcgtattggc tgataatgag caaatcagtc agcaaatgag
    48781 ccaagtcaaa acagatgatt ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa
    48841 agagctttcg gaagaaaaaa tcgatgcgta tttagtagtc tcaactgaga atgataagat
    48901 aaatgggatg ctttatagcg aaaattcatt aggacaaagt acacaactac tgatacaaca
    48961 acaactgact ggcttgcaat cgatgcttcg agcgagtcaa ttaggcattt caccagaaga
    49021 agtagctagc ttgagccaac aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa
    49081 aggaaaaatg acagtcggag aagataactc gacagtccaa tacgtcgtaa gttatgtagc
    49141 aacgattgtc ttgttcatta ttattttgac gtatgcgcag atcattgcac aagagatcgc
    49201 ttctgaaaaa ggcacgcgga tcatggaagt gatcctgtcc agtacgactg cacaaaagca
    49261 tttttacgga aaattaacag gtgttttact tgttgcatta acgcaaatgg cattatacgg
    49321 tattattttt ggtataggat acagtcaatt caaagatatg gaaatggtca aaaatatatt
    49381 agacggtatc tcattggata acttgattgg accattcttg tggtattctc ttctattcat
    49441 gatctttggc attttgattt tttctgtttt agcagcttta tgtggctctt tggtcaacaa
    49501 agcagaagat acagcaaaag caattttgcc agtgacttat ctatctctag gcggatacat
    49561 gcttggattg atactaggag cttcagatcc aaacaatatc gttattcgga tcacttcgta
    49621 tattccattc ttatcttcat atattatgcc ggttcgctta gcaaatgaga cagtcggtat
    49681 gggtggagca ttgatttcct tgttggtact tgtagccgta acattcttat tgatgatctt
    49741 gtctgcaaac atgtacaaat cgaatgtact cgtttatagc gaaggcggat tatggagttc
    49801 attgaaacaa tcgatttcaa ttatgaggaa tgaacgaaaa aaagcataaa acaatcctaa
    49861 aatacagaag gaagctggac atctattgtt cccagcttct ttttgtatag acctatttta
    49921 tttattttac tatattggat atatattcat tgaaaatagt gtataacaac tttataatat
    49981 tgagtatacc aatttttgga ggcgttttaa tggaccgtta tttagattta ctttcagaaa
    50041 aattcccctc aacggaggat gtcatcacgg aaatcatcaa tctcgaagca attttgcagc
    50101 ttccaaaagg aacagagtta tttttgagtg atatacatgg cgaatttcct gcttttgatc
    50161 atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt ttttctgaac
    50221 aaatgacgga aacggatatc agccaattca ctatttttac ggcttatcca gaatatgcat
    50281 taacgactga ttggtacaaa gaacaagata aatcgcagct gatccatcat ttgatcgatc
    50341 ttttacggtt tacgacagtc aaatataccc ggtcaaaagt tcgaaaagga ttgccgaaag
    50401 aatatagcta tatcattgag gaactcttgt atatcgatga ccgtatcaat ggaaaaaaag
    50461 actatgtaaa aaaaatcatt gaacaacttg ttttgatgaa ggaagaagaa aaatttttaa
    50521 cgaaacttgc acagacgatc caaaaatctg tcatcgatca tttgcatatc gttggagata
    50581 tttttgaccg aggaactcaa gcagccaaag tgatggatcg aatcatggat ttttcttctg
    50641 tagatatcca atggggaaat catgatattt tgtggattgg tgcgtattgc ggctctgagg
    50701 catgtttact gacactttta cggattgcag cacgctataa ttatttattt gaattagaga
    50761 aagaatatgg gttgaattta cgtccattgt tttcttttgc tcatcaaact tatcagaaga
    50821 atccagtatt tgcgcctaaa aacagtaaaa atctatccga aagagagcaa gaagaattag
    50881 aaaaaatcca tcaggcactt tctatccttc aattcaaatc agaacatcag ttattggatc
    50941 gtcgacctga atttaaaatg gacgatcgta aattgctgga aaaaatcgac tatgaagcat
    51001 caacgattga tttagaaggt cagctctatg agttgaaagg aacttgtttt caaacgatcc
    51061 agccagatga tccaaaaaaa ttcttggaag aggaacaaaa agtgattgat agtttgatgt
    51121 attcattcca gcactctttg agaatgcaaa aacatatgac gtttttgatc gaaaaagggg
    51181 gaatgtatct cacgaacaat cagcatgtgc tgtttcatgg ctgtatacct gtagacgaga
    51241 agggacagct cttagcattt gaattagacc aatcttatcg cggaaaagaa ctgctgggct
    51301 tttttgaaaa acagataact gaaagcgcaa aagatctgac tgcaaaagaa gatttagcaa
    51361 ccgatttgat ttggtatgcg tggagtgggc cattttcgcc tttattcggc aagagtaaaa
    51421 tggcgacttt tgaacgttat tttctcacag agcctcatac gcacgtcgag aaaagcaatc
    51481 cttattatca cttgcgagat gaagagtggt tcagtgaaaa aattttagca gaattcgaca
    51541 caaaagaaga agggtcagca atcattaatg ggcacacgcc agtaaaagta aaaaaagggg
    51601 agtcaccgat caaagcaaat ggaaaagtat ttgtcattga cggaggttta tccaaagctt
    51661 atcaaaaaac gacaggaatt gccggttatt cactattatg caattcttat ggtttccaaa
    51721 tcgtgacaca ttatccgttt ttgcctattg aacaacaatt tgcaaaaaaa tccgaccaaa
    51781 caaatgtgaa aaaagtaatc gaacagcaac taccacgcaa actcaatcgt gatacaacaa
    51841 aaggtatgga actgcagcag cagattaccc agctcaaaag gctgttggac tatcgaaagg
    51901 acgattgaga agaattccag tgaacattgt tccatagact ggagactgag acaaaagtcg
    51961 tgtcgcagtc tctttttccg gataaacggt gttcttctgc caactcctcg gtcacaagtc
    52021 acactattca acaaacatga aaagctgttt tttgaaattg ctccttgtga ctcagagttg
    52081 aacggcaggc tcacgacctc ttggtataaa cggtgttcaa aagccagttt ctcggtatta
    52141 agccaaattt atgcaaaaat gtgaaaagcc attttcacaa aatttttctt aatactcgaa
    52201 actaaacagc tttttcacaa ccccctcaga aagtctatta aaattcattc acaaagaaat
    52261 cgaataagcg ctgttcagat ggatgtacac ggtgggtaag ttccggatgc cattgaacac
    52321 cgaggatttt atgcatatca tctttgcttt gaaccgcttc aatgagtcca tcgttagaaa
    52381 aagcgatggc ttttaataaa ggtgaaagat ctttgactgc ctgatgatgg taagaattca
    52441 caaaatatgg atcggggaga aggttgctta acaaactgtc tgaaacaatc ttgacttcgt
    52501 gagtcgtaaa ttgcggggca gtgggctgtt gttcatgttt tactgaccac tttggatata
    52561 atgaaagatc ttgataaagt gtaccttcca aaacgacatt caatagttgg agtccccggc
    52621 agactgcaaa aattggtttt ctttgtttca atgcttccaa gatcagtgcc tgttcaaatt
    52681 ggtctcgttg aatatttgtt tccgttagct ttggatgagg atcttccatg taaagttgtg
    52741 gactgacatc ttgaccacct gcaagaataa gtttgtcgat ttgtccaatc agttgaggag
    52801 ctgaatctgg agacatgact ggcagaataa gaggcaatcc ttgagcatct aaaactgcat
    52861 ccacgaatcc ttgcggtgta tagctcactt gatttccttg gaaagtatct gttgctcgaa
    52921 tcagttgatt tccggcaatg ccgatgattg gtttcatttg tattccccct aaagtagact
    52981 tattgtaaca aggatttaaa aaaaattaaa ctatgtaaga cttgccccta tacttatctg
    53041 ttcttgctgt gttactatct gtaaagagaa atttcgtgtg aaattcatga cgatagttga
    53101 aaatattgct taatttgtta taataaaaag taagcagaaa taaaaaggtt gggcgtaagc
    53161 ttcaaggatc tagttcttca aggggtgaga aggacatttt attgaagggg cctgcctata
    53221 ctaagttatc tttagaggta catactttca atgaaaaaag aaagcatgta cctctttttt
    53281 aaatcgaaag gaagtgaagc aagtgttaga agctttggaa caaatccgaa aagcagaaga
    53341 agaaaatgaa aagcgtgctc aaacactgcg gcagcagctg aagatctatg caacagaaaa
    53401 agagcaggaa ttggaaacga tccaaacaaa ctatcgacaa caactaaaac agttggtgga
    53461 agaaaaagaa caatttgaaa tacaagaatt agaaaaagaa aaaatgaaat tgaaagcaga
    53521 agcagacaag gtgcaagcaa ctttgaaaga tcaatatcaa aagcatcaga gtcaagcgat
    53581 cggtgcgatc attgaaaggg tgaaagaaac ttatggccgt cactaagatg gaaaaagtga
    53641 ccctcatttc agataaaaga aatcaggaga ggattttaca agcagtacaa ggaatccaaa
    53701 acgttgaaat ccgggatctg tatcaaaata gaacaaacaa ccagtgggtg gagaagtatt
    53761 ttccagatac cacgattatt gataaagaag caaaattaag cgctttaggt aatcggttga
    53821 aagacatcca agaagctatc caattcatac aacaccacgg cgatagccgt caaaagaaac
    53881 tccatttgaa acgtcaagaa ctttccttac aagatttgga agccaattat tccgaagaaa
    53941 atttcataaa taaattgaaa gaagtccttg atttaaaaga aaagtgggaa caactaactg
    54001 aagaacgtga tcgtttagaa gaagaggaag aatggcttct tcattggcaa cagctggata
    54061 ttgctccaag tatgtatgca agtaagacga ccatttttga gatggggact gtgagtgcag
    54121 caaattttga aagtttcaag gaagaactga gtaagcttga tgacgtttat atggaagaaa
    54181 tagattatac gccaaaacaa gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg
    54241 ttatagaaga aattgctggg aaatacgggt tcaataaaga aacgtatcct tatgaacaaa
    54301 atcctaaaga aaaactgcaa gagaccaagc agcaattatc cattgttcac gagcagcaaa
    54361 agaagataac gacagcgtta ggtcagtgca gtggctatat ccaagaattt gaatgggtag
    54421 aagaagtcac acttgccgtt gcagaacgag aaaaaataaa agaacggttt atccatgcga
    54481 cttatctgat cgttttgcaa ggttgggtag atgcagaaga aaaacagaat ttgctgcatg
    54541 tgttaaacga aaccgtatca gaagaagata tatacgtcag tttcgaaaca ccaaactcag
    54601 aagagattca gcaggaagtc cctactaaat tgaaaaacca tccgcttgtc gaaccttttg
    54661 aattgctgac tgaaatgtac agtctgccaa aatatgaaga agtagatcca acaccttgga
    54721 tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc agatgttggt tatggtttgc
    54781 tgatgctcat tgggacaata ctagctcaaa aactacttgt tctaccacgc gggatgaaac
    54841 gtttcgtaaa attctttgag attctctcga taccatcgat catctgggga ttgatctaca
    54901 gttcattttt cggacagtca ttaccaaaag aactgtttgg catccgcttg cctttcccaa
    54961 tcctatctac gacggatgac gtcaatacga ttttgatttt atccgtgatt ttcggtctga
    55021 tacagatttt ggttggatta tttgtggcag caaaggaaaa tatcaggaga aaagattact
    55081 tgagtgctgt cagtgatgga tttgcctggc aagggatcct aattgggatc gttattgcgt
    55141 tggttggtgc catgttgata aaaaatacag catttgttta ccttggcgct gggctatcga
    55201 tcattgctgc cttatgtatc ttgatcgttc cgattatcca aacgccgtct aaagtcaaag
    55261 gtgctgccaa aggtgcgtat aatctttacg gactgaccgg ctatatcggt gatttagtca
    55321 gttatacacg tttgatggca ttgggtattt caggcggaag tattggagcg gcattcaata
    55381 tgctagttgc tttcatgccg ccagctgctc gattcagtgt agggatcctt ttgatcatcg
    55441 ctttacacgc actgaatatg tttttgacac tgctaagtgc ctatgtccat ggcgcacgat
    55501 tgcagtatgt cgaatttttt gggaagtttt atacaggagg cggacgtgcc tttgatccgc
    55561 tgaaaacggc ggaaaaatac gtcaacatca atcacaagaa aaaaaacaat aaataattgg
    55621 aggattttta tataatgatg gattacttaa ttagtcaaaa tggtggaatg gtatttgcgg
    55681 tattagcgat ggcaacagcg acgatttttt cagggatcgg ttcagctaaa ggtgttggaa
    55741 tgacaggtga ggcagcagca gcattgacaa caagtcaacc cgaaaaattc gggcaggctc
    55801 tgattctgca attgcttcca ggtacgcaag gtttgtatgg attcgttatt gcctttttga
    55861 tttttatcaa cttaggaaat gacatgtcag ttgtacaagg actgaacttt ctaggtgctt
    55921 ctctgccgat tgcttttaca ggcttgtttt caggtattgc ccaaggaaaa gtcgcagcgg
    55981 caggtattca aattttagcg aaaaaacctg aacatgctac aaaaggaatc atttttgctg
    56041 cgatggttga aacatacgcg attttaggtt tcgttatttc cttcctatta gtgttgaatg
    56101 cctaagcata aaggaggaaa agcagatgaa cgccattgaa aaaatcatct ctcagatgaa
    56161 cgaagcagca gagcaggaaa gagcggcgtt agaacaagaa gagcgaatga agatcgatca
    56221 aaactttgaa caaaaacgta cgcaagttga aacagaacat caaaaacaaa aagaaaaaca
    56281 aatcgaatta ctcgaaaaaa aataccgtca gctaaggaat cgccagcaag tagaagtacg
    56341 acaagaaaat ttgaatgcga agcaggaatt tttaagacga ttgtttgcag atgcggtaac
    56401 tgaaatggaa aattgggatg aatctgaaca aatccagttt atcaaaaatg cattgtattc
    56461 attacctttg acaggaaaag tagcattcat tgctggagaa aaatcagcag cctatctttc
    56521 tcagacgttg ttggatgaat ggaacaatga acttcccttt atgatggttt taagtgatga
    56581 aactgttgct gatcaagcgg gttttctgat aaacgatcaa ggagttcagt ataattttct
    56641 tttcagcagc cttgtacaag atatccaagg aacgatgagt tttgagattg ccaatcaatt
    56701 attcgaataa ggagggatca gtgtatggaa taccacgaac taaatccctt gattcgcgga
    56761 agagagcttg aacttttgtc caatgagaca tttgaacgga tgattcaaac aaattctgtc
    56821 gaagcacttg gtgaaatatt gaaatccacg atttacagtc catatattta tgacggatat
    56881 gaatatgact tcgaacagaa tcttttaaag gagcagagcc gtctatttcg ctggttaaag
    56941 gaatcagcac cggagcctga aatcgtttgg atctatacga tgcgttatac tttccataat
    57001 ctgaaggttt tgacaaaagc tgagatgaca aatcaaaatc tggaccattt gtatatcgac
    57061 gatggatttt attcattaga aaccttgaag gatgccattc atacgcaagt ttcaaatgaa
    57121 cttcctgaaa gcattatgga cagtatacga gaagtacgtg attattttga agagtctacc
    57181 attcttcagg gaattgacgt gatttatgat cgacagtttt tgacagagca gcgacggctt
    57241 gccgataagc taggatatca agaattgctg acagaagtca ttgcttttat tgacttgacc
    57301 aatatcacaa cgatggctcg aggaatattg cagaaaagaa catatgggtt catgacgacg
    57361 gtactttcca gctcgggaag tatcgaaaaa gaagtatttt tgaactttgt agataaagat
    57421 atggaagatt acacgaattt tctattagag acagattatg ctgattttct gcgccctgtc
    57481 atccgtgagg gaaatattga tctcgtaaag cttgaacagt tgaaagatga ttatctaagt
    57541 tcattgtatc aagaagcaca aacccaggct ttcggaccac tgccactact agcctttttg
    57601 aacgcaaaag aggtagaaag caaaaacctt cgtttactgg ttatcggcaa aagaagccat
    57661 ttcgaatcag aaacaatcag aaagcgggtg agacaagtct atgacgcata agatcggtgt
    57721 agttggcgac aaagattctg ttttgccatt caaattgttt ggctttgatg ttcgttatgg
    57781 tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt
    57841 gatttatgtc acagagcaat gtgcagcatt gagcccagaa acgatcgaac gttacaagga
    57901 acaaatgata cctgctatcg tattgattcc caatcatcaa ggatcattag ggatagggct
    57961 ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa
    58021 ggagatagaa attttgcaaa ttggaaaaat cataaaagtc tccggacctc ttgtaatggc
    58081 tgagaacatg tctgatgcca gtatccaaga catgtgtcta gtgggagatc taggagttat
    58141 cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt caagtatatg aagagacttc
    58201 tggaattggt ccaggcgaac cggttcgctc tactggtgaa gctttatctg ttgaattagg
    58261 accagggatc atttcacaaa tgtttgacgg aatccaacgg ccattagata cctttatgga
    58321 aatcacacaa agtaatttct tagggcgcgg agttcaattg cccgcattgg accatgagaa
    58381 aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg gtgagtgctg gagatgttat
    58441 cgggatcgta gaagaaacaa aagttataaa acataaaatc atggtgccaa atgggataaa
    58501 aggaacgatc aaaacaatcg aaaaaggttc attcactatt gatgaaacag tatgtgtcat
    58561 tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa aaatggccag taagacgtag
    58621 tcggccaatc aaagaaaaat taaacccgga tgcacccatg atcactggac agcgagtgat
    58681 tgatacattc ttcccagtga caaaaggagg agcagcagct gtacctggtc cttttggtgc
    58741 agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc gatgtggaca tggttgtcta
    58801 tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt ttaaatgaat tccctgaatt
    58861 gattgaccct aatacaggag aatcgttgat ggaacgtacg gtattgattg ctaatacatc
    58921 aaacatgcca gtagcagctc gagaagcttc tatctatacg ggaatcacaa ttgcggaata
    58981 tttccgtgac atgggttatg acgttgctat catggcagac tccacttcac gatgggcaga
    59041 agcacttcgt gagatgagcg gacgtttgga agaaatgcct ggtgatgaag gttatccagc
    59101 ttatcttggt tcacggcttg cggaatacta tgaacgatct ggaagagtca ttgcattagg
    59161 atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct gtatccccgt ctggagggga
    59221 tatttcggaa ccagtcacac aaaatacatt gcgggtcgta aaagtattct ggggtcttga
    59281 ttccagttta gcccaaaaaa gacattttcc ttcgatcgat tggatacaaa gctattctct
    59341 ttattcaaca gaagtaggga agtatatgga tcatatcctt caagctgact gggcatccat
    59401 ggtcaccgag ggcatgcgta ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt
    59461 ggtagggatt gattcccttt cagataatga ccgtctgaca ttagaagtgg caaaatccat
    59521 tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc gatacattca cttcaagaga
    59581 gaaacaattc aaaatgttga agatgatctt gacctttggt gaagaaggac gccgtgcgtt
    59641 gtcgttaggt gcttatttaa gtgagatcat ggacggtacc gtatcgattc gtgaaaggat
    59701 cggacgaagc aaatatatcc cggaaacaga attagaaaaa ctagatcaaa ttattggtga
    59761 aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc atgagcaatg attaaagaat
    59821 atcgtacgat cggtgaagtc gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca
    59881 aatatgaaga attaatcgaa gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat
    59941 tggaagtcca agaagataaa gcaatggtcc aaatattcga aggaacaagt ggtatcaatt
    60001 tacgtgactc ttctgtacga tttttaggcc atcctttaga actaggtgta tcggaagaca
    60061 tgatcggtcg agtattcgat ggtttgggac gaccgaaaga taacggacca gagatcctgc
    60121 ctgaaaagca tttggatatt aacggagaag taatcaatcc agtagctcgg gattatccag
    60181 atgagtttat ccaaacaggt atctctgcaa tcgatcattt gaatacatta gttcgtgggc
    60241 aaaaattacc agtattttct ggctctggtc ttcctcataa agaattagct gctcagatcg
    60301 cgcgacaagc aacggtactc gattcatcag atgatttcgc cgtagtcttt gctgctattg
    60361 gaatcacttt tgaagaagct gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg
    60421 accgttctgt catgtttatg aatttagcaa acgaccctgc aatcgaacgt atcgctactc
    60481 ctcgtatggc tttgactgct gcagaatatc ttgcttatga aaagggaatg catgttttgg
    60541 ttatcatgac agacatgacc aactatgcag aggcattgcg agagatttct gcagcacggc
    60601 gagaagttcc aggaagaaga ggatatcctg ggtatcttta cacgaactta gctactttat
    60661 tcgaacgagc aggaagaatc cgcggactaa aaggatcggt tactcaaatt ccgattctaa
    60721 caatgccaga agacgataaa actcatccaa tccctgactt gactggttat attacagaag
    60781 gacagatcat cttgactaga gagctttaca agagtgggat tcagccacca attgatgtac
    60841 tcccgtctct ttcgcggtta aaagataagg gaactggacc cggaaagaca agagaagacc
    60901 atgcagctac gatgaatcag ctttttgcag cctatgcaca aggaaaacag gcaaaagaat
    60961 tagccgtagt tttaggtgaa tctgctttat ctgatgtaga caagatctat gcgaagtttg
    61021 ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta cacgaaccgt tcgattactg
    61081 aaacacttga tctagggtgg gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa
    61141 tcaaagacga catgctggac aaatatctgc cagaagggaa gtgagttctg atggcacgat
    61201 tgaatgtgaa tccgacccgc atggaattga ctcgattgaa aaaacagttg acaactgcca
    61261 caagagggca taaattatta aaagataagc aagatgaact gatgcgtcaa ttcatcctgt
    61321 tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt
    61381 ctgattttgt attggcaaat gcttcgatca cggaaccttt catcgaagaa ttatttgttt
    61441 tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac
    61501 ctgtgatgaa ttttgaatat gatgaacagc tgacagacac acctttggaa tacgggtatt
    61561 tgaattccag tgccgaactg gatcggtcaa tcgatcggtt tactaccctg cttccaaaac
    61621 ttttggaact gacagaaatc gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga
    61681 cacgtcggcg agtcaatgca ttagaatata tgacgatccc tcaattagaa gaaacgatct
    61741 attatattcg tatgaagctg gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag
    61801 taaaaaatat gggtaccact gactaagtgg tatctttttt taaaagagga aaacgatgat
    61861 taaacaaagc aaacaaataa agaaacgttt gccggctgtt cagcttctcg cattaggatt
    61921 ttttctcctg attttagttg gcggcggcct acttacccta ccatttttta gtaatagtgg
    61981 gcatgggaca aattttatcg atgcgctttt cactgcaact tctgctgtct gtgtaacggg
    62041 attgacaaca ttgaatacag cagagcattg gaacagtgct ggtcaatttt tgatcatgct
    62101 tttgattgag atcggcggtc ttggttttat gatggttccg attctatgct ttgcattgat
    62161 aaagaagaaa gtcagtctca gcacacgtat tatattaaaa gaagcgctga atctagaaga
    62221 agtatcaggt gtgatgaagc tcatgcttta catcctaaaa ttggcctttg tgatccaatt
    62281 gattggtacg gttgctttat cttttgtatt tgtgcctgaa tttggctggt tgaaaggtat
    62341 ctggtacagt ttgttccatg caatctctag tttttgtaat gcagggtttg atttgttagg
    62401 agacagttta gccaatcacc agcaaaatgt ttatttgatc atggtggtat ctgctttgat
    62461 catttctggt gggttaggct tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa
    62521 gaagatgacg attcattcaa aaatcgcatt aaccgttaca ggtacccttt tattaggcgg
    62581 attcgttgtg ttctttttca cagagcgcaa tggggcaacg ctagtggaag ggaatatgtt
    62641 tcaacgattg gcaaacacct tttttatgag tgtgacacct agaacggctg gatattattc
    62701 agttgattac ctccatatga gccatgcagg tctgatttta acgatgtttt tgatgtacat
    62761 cggcggaaca tctgggtcga cggccggcgg attgaaaaca actacgttag gtgtgttgct
    62821 gatccagatg cgttcgatgt tgaaaggacg tacgagagcg gaagcatttg gccgtaccat
    62881 ccgacaaggc gcagtgttgc gggcacttac tttatttttt gttacgttga cgttgtgtgt
    62941 agtcgccatc atgattttat cggtgactga aacgatacct aaaacttctg gcatcgaata
    63001 tattgctttt gaggtcttct ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc
    63061 agacttgact gcaataggga aattgatcat tatagcgctt atgtacattg gacgagttgg
    63121 tgtattgact gtggtcttct ctctgatgac aaaagcacaa agaacacaat caaaatacaa
    63181 atatccagaa gaaagtgtca tgattggata atttcatctt ctgacagaaa cctagtgatt
    63241 tagtaaaata agaaaataaa ataatttttc ttgcttcttg gatactttaa gtgcaaaatg
    63301 ttcgtttatt ttttgaggca ctacccgttg ttgaacctcg ggtagtgcct cttttttatt
    63361 gaaaggaatc aataagattt gattccttta tttatcaata ggttataatt catatttttt
    63421 tattttttct tttacgatac ttagtccaga agctaaaatt tcaaggttat tagaggatag
    63481 ggaaattcga acgaatgcat ctggttgcgt acgtgtagta aagcgtctgg aatgataaag
    63541 tcggataccg ttagcaagaa agtcattctc aattatctta gggtcaatct caggatcaac
    63601 tggaagaatt cgatagtaag ggacacgatt cgttggtttt tctactttga aaagagaatc
    63661 aaaaagctga ttggctcttt ttgtaagact taatttagtt tctaagatcc tatttgcttc
    63721 atttgaatgg aggatttgag tgatgacttc cacatctaat ccagaagttt tcacatttat
    63781 attaaaaatt gcttgttcta tcttgctttg gacatgtact ggaactacaa gataagcaac
    63841 tcgcaatcca gaacaaataa attttgtcat tccagctaag tagattgttt ggttaggtaa
    63901 taaattttgg aaaggaggaa tgatcgtatg ttgatagtaa gttgttaaaa atgaatggat
    63961 atcatcttca ataacccata gaccttcttt ttcaatcact tcggctaatt gctctcgtct
    64021 tttgtgagag atttgaaacc ctataggatt attacaagaa ggcataagaa agacaccatg
    64081 aatcttcttt tttctacatt cctgaatcaa tatatcaggt cgcattcctt gctcgtcaaa
    64141 atcaattgga acgatctcta aatgatataa ctgagccaat tcaatgaaat tagcgtatgt
    64201 atatcggtct gttgctattc tatcacctgg agagaaaagt gcagaaagtg tgatagccaa
    64261 tccattttga acaccggaaa caatagatag agattctaag gacggaatga ctccttgagt
    64321 ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg cctttgggat cttggtaatt
    64381 aagcaaacca acaagatttg ggttattact agttgattga ataaacggga caacgaattt
    64441 attacattct tcaaaagaac taaccagacc aaaatcaatg atatgttcgg ttacttcatt
    64501 ggtagagatg gtgatggact caaagacatt gggagaaata aatgtgccac ttccaatatt
    64561 cgtatacagc aaacctttct caattccata tttataagcc ttgccaacgg tagtaaaatt
    64621 taaatctaaa taatcagcta gctctctttg ggagggtaac tgagtgtttt tctgtaattt
    64681 attctttaaa atatcttgtt ctaattgttg aatcaacgat cgataataag gacgcttcaa
    64741 tttttgtttg tctggtcgcc aagttaacgg gtaattgtca taagaattaa taggcatttt
    64801 ctccactcct taaaattgta taccatacaa ttttaccatt gattgtattg attttagaag
    64861 ttaatctttt gtgaggaggg aattgtctat gaaaattatt aattttgagg aagtagtagc
    64921 ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa tgtttttttg attatgaaag
    64981 tggtaaaatc agccaaaatc ctcgattggt ggaaatttta cctgacggaa aaggcaaaaa
    65041 tatgtttgcg atgatgcctg cttatttggg ggagaacaga tattttggca caaaagtcat
    65101 taccgctttt ccggataatc atcagtatca tttaccttct catttaggag agattttctt
    65161 gtttgatgct caaaacggac tacccaaagc attgataaac gcaaatgcag tcacctggat
    65221 tcgaacagct gctgtatctg cgctggcaac agattactta gcaaatccgc atgcagaaac
    65281 acttgcttta attggagctg gtcaacaagc attgtcgcat ttgactgcga tttctaccat
    65341 tcgtcccatc actacagtgt ttgtttacga tattttagaa gaaagacgaa atacttttat
    65401 cgctaaagct aaaaaacagt atcctaaaat aacgtttata aatgcaatat cagtagaaga
    65461 tgcagtacgt ccagcggaaa tcatttgtac gttgacctct agtaaagagg catttcttca
    65521 aaaagagtgg gtaagggaag acgcacatat caatgctgtg ggcacattta ctcctgatac
    65581 aagagagata agtacatcgt tgatggaaac ttctcaaatc tatgtagatg attactcagc
    65641 tgccttaaca gaaagcgggg acctgcttat agccatggca gaaggagatt tagaggaatc
    65701 ttgtattgtt ggtacattag gcgaattagt ttctgagaaa aaaacatttt ataaaggaga
    65761 atcgaggccg acgattttcg acgcagttgg tctagctatt gaagatcttt gttgtgcaga
    65821 atatatttat aacaaattag agaaaggggc tttaaaatga acttagatta ttacgtagtt
    65881 gatgcatttg cagatgaggt gttcaaagga aatccagcag cggtttatgt gctagaagaa
    65941 tggctgccag aaggtacgat gcaaaaaatt gccattgaaa acaatttatc tgaaacagca
    66001 tttacagtga agaaaaacca agaatttgag cttagatggt ttacgccaga tcgagaaatc
    66061 gatctttgtg gtcacgcgac attagcaaca gcatttgttt tatttaacta ttacaaaata
    66121 cctgatgaaa cgattaaatt ttctactcaa agcggtaatc tatttgtgac aagaattaaa
    66181 gattattatt acatggattt tcctagtatt atgccgaaaa aagtgccgat attgtctgaa
    66241 tatgaagaag cgattggggc taagattaaa gaagcctact tagcacgtga tttatttttc
    66301 gtattagaag atgaaaaaac ggtggctaaa ttgcagccag attttacagc tatcaaaaat
    66361 tttgaattag gaataggagt catcgtgaca gcagaaagtg tgcaaaaaga ctttgtttct
    66421 agaaccttct ttccaaaatt aacaatcaat gaagatccag tatgtggatc agcacattct
    66481 aatcttattc cttattgggc agaaagaatg aataaaacaa aattgtcagc ttatcaaatg
    66541 tcatcaaggg gcggggatct agaatgtgaa ttaaaagaag atcgtgtgat aataggagga
    66601 aaagcagttt tgttttctaa aggtacctcg tttatttgat ttctttcaaa tgaattaaaa
    66661 ataaattggt ggagagtggc aaccttatag ctacaatggt tgttaaaaaa agacgcaaac
    66721 tttttcaatt taatgaaaag tttgcgtctc attttaatta ttggtgtttg ttttttattc
    66781 aatcaagagt aaaatgcccc atactaacta tcctcggatg acttcgattt tataaccatc
    66841 agggtcaatg acaaagtagt aagaaggcgc agtgccaggt aatcctttta agtcagtaat
    66901 ctcaagacca gcagctttat gttgctcgtg taatttttct agatcatcag ctgctatagc
    66961 gatatgacca tagccgtctc ctagatcata cgcaccatga tcatagttgt aagtcaactc
    67021 taattcatag tcatccccag gaagtgtcaa ataaacaagt gtaaatttgt tttctggaaa
    67081 atcacggcgg cggctttctt caaaaccaaa tgccttttga taaaattcga ttgatgcttc
    67141 aagatctttc actcgaacac atgtgtgagc cattttcata gattcatctc tccttttcct
    67201 tttactcaat cataacaaaa agaagtaagg aaatagttga tttgtgcttg ttcatgagaa
    67261 atttctcatg taaaatggat agaaaataaa actgttaagg tggaaactat tcatggataa
    67321 accaattttt ggtgaaaaaa agcaatttgc tgattatcaa acccgttatg cagcatatat
    67381 tgtcatagaa agagatcagc agattgcagt aatcgaggct ccaaatggtg ctttttttct
    67441 tcctggcggg gaaattgaag gagaagagac gaaagaagaa gcgattgtcc gtgagctgat
    67501 agaagaaatg ggaattgtgg cagaaatttc ttcttatctg ggagaagctg atgagtattt
    67561 ttactcgaat tatcgtgcaa cttattatta taatccagga tatttctttg ttgctgattc
    67621 ttggaagcgt ataggagaac caactgaaaa gaccaataaa atttggtggg tgagccctca
    67681 agaagcgctt gtgaaattga aacgtggtag ccaccgatgg gcagtcgaga gatggatcga
    67741 aaaaaataaa aactgaaaga aagcgagtag atcagctttc tctcagtttt tttatttatt
    67801 acagtacata ttttttgatt gcttcagcta caccgtgctc gtcgtttgat gaagtgatga
    67861 catttgctgc ttctttgaca agagaaacgg catttcccat tgctactcct agaccagcat
    67921 attcaatcat ggataagtcg ttctcgttat ctccgatagc catgatttca tcttgatcaa
    67981 taccaaggtg ctgtgcgaga ttcgcaactg ccgcaccttt acttgcttct ttatttagta
    68041 cttcgaagta atagggcgca cttttgaccg ttgtgtattt atcacggaaa gtttgcggta
    68101 aacgcgcaat tgctgcatct aaaatttctg gttcgtcgat catcatcatt ttgacgatgt
    68161 tcatgtctgg tgtcatttct tcaggtgttc gatattttag cggcatcttg actaaacttg
    68221 cttcgtggat cgtataagcg ctgatatctc gattaggcgt gtagatcgct ttgtcatcga
    68281 tcgaatggag atgactgccg acacggcgtg ccatcacttc gatctctagg aaatcttcat
    68341 gtgtcagtcc gtgacgggcg atgattttac ctgatttcgt ttgttgtacg agtgccccgt
    68401 tataagtaat aacgtaatca ttatcttgaa aaaggttcaa ctcgtttaac tgttcctgga
    68461 cacctggtaa gggacggcca gtgcataaaa cgatcgaaac accttttgca acagcagcgg
    68521 caatcgcttc ttttacttct tttgttactt ctcttttctc atttaataaa gtaccatcga
    68581 tgtcgatagc gactagtttg atagacataa tttcctccaa tatattttaa tttatttcaa
    68641 tcaatgcgcc attacggata tggctggcaa attcttgata agtttcgtca aataaatcat
    68701 aatgatcacg caaggacgga tcaaccattt cttttggaaa gaaaaatcgc tcatctcctt
    68761 gttcttgtcc cgctaatgca gcaacaagct gactaacctg tgataattct atcaaagttc
    68821 cgtcatttcg caaaatctcg atttgtgtgc gatgtctgcc ttgttctggg cgataaaaat
    68881 cataaggtag atcgtagcta gaattcacag cagtgtaata aacaggatta tatccgacct
    68941 tttctactaa caaagtgagt tcttggacta attttgaatc ccggttgcca gaaaaagtag
    69001 cagatttcag tggtttacga ttcaaaaaac gtttagctaa atcatttaaa ataggatcag
    69061 cagattcaga ccattgagta aagtaagtgc ccaagacccc atcatcaagt ttgaggtatt
    69121 cttctaaagt aaagcttccg tcaagaaatg gcagaagtaa ttgagaatgg agttcaaaac
    69181 tgctggcatc ttcttgatac aactcgtgtg cgcgatgaag cagatgctcc aaaattactt
    69241 ccataccacg ggaaacagga tggaagtaca cttgcacata catttgatat cggctaacga
    69301 tataatcttc cactgcatgc atgccactca tggaaaaagc gatcccgcct tcataaggac
    69361 gaatcacacg taatattcgg gtaagatcaa atgttccata ttctgttcca gtgaaataag
    69421 catcccgaag caaatagtcc attcgatctg catcgatctg actagaaatc atttggacaa
    69481 cttgtgggtt ggggtaagtt ttttggatca cactagctac cttttcagga aaaccttctt
    69541 ctactcggtt aagaatctga tacacttctg tttctggaga agtgatgatc tctacagtga
    69601 tggcttcatg attcgtatgg aagatatgtt caaatgtatg agagtaagga ccatgaccga
    69661 catcatgcaa cagtgccgca cataaagtca ccagccgttc ctgatcatcc cagcctccgt
    69721 tgcctatttt ttcttttgaa aaatttcgag aaaagatatc gcagattcgt cttgaaatct
    69781 cataaacgcc taatgaatga gtaaaacggc tatgttctgc tccgtgaaaa gtgaaagaag
    69841 aagtgcctaa ttgtttaatg cgtcgtagac gttggacttc tttcgaattg atcaaatcta
    69901 agattacttg atgctggaca tggatatagt tatggactgg atcacgaaaa actttttcca
    69961 taggcagcat ttgatattta taaggggttg tcataatgta gctcctttat gttattacgt
    70021 tgttccattc tctggatttg gttgttccat tgctccagct ggatttcttt ttttagccag
    70081 tcggtagaat cctgtaaatc acttgcagaa tacagctttt caaaagcttt tgcaaatgct
    70141 tctttgactt cttttacaaa aaggggttgg tcaagcagtt cttcaagtgt tgccattgta
    70201 gcaggatcca ctgcaggata gccatttgta ccaaagtctt cttttaaagc agattgataa
    70261 aatcgcttca tgacttgccc gcgtttttcc tgattaccgt tgacacttaa gtacatcata
    70321 acagaaatcc catttttgat ccgacgctga gcgataccgg caaatttttt gccatggatg
    70381 cttaaatcat aagttccagg acaataagaa tgggtgattt cgaatgcttg tatcggtgcc
    70441 tttggaaaag cgtatttagt caataggagc atttgctcgt aagcgtcatc tatggttatt
    70501 ttttttgtgt cactttgggg gaaaatcaaa gaaatattca aaattccttc atctgatacg
    70561 atccctaatc cacctgaatt tctgatcact ggagcgtaac ctgcatcttt cagtgtttgg
    70621 atcccgtcac ttagaaaagg agttcgaaca tccttcatac ctaagatgac tgtgcagtca
    70681 tattgccaga aatgtaaaaa gatttgctgg tgttctttag aatattccgt taatatatct
    70741 gttaatgcaa aaggggaaaa gtcgtttttt tgataaatgt gctgatctag tcgaatcgca
    70801 gggttcattc aaccggctcc tttgtataaa ttcatatttt ctacaatatt atactctaaa
    70861 atcaaagagt tgttttggaa aaaaggtttt ctgtaaaaat ggacaagttg gttgacaaaa
    70921 caaaagaaac taggaacaat gtagctatga gaagtaaagg agaatgaata atggaattat
    70981 cacaaggaac accgatttca atcaaattgc gtacaaaagt aaaacagcaa ggggaagtgc
    71041 aagattttta ttttgatttg aaagggcaaa tggtgacaat tggcgatacg ctgtacatac
    71101 gttataaaga gattcaggag gagacgggcg atgagattcc tgtaacgatc aaattagttc
    71161 cagatggtca tgtacagctg atccgtgcag gagaaatgcg gatgcgtttg aaatttggct
    71221 ataaagaacg gttagaaaca tcctatcgaa caccatacgg tatgatccag attgctactt
    71281 ttacgaaaga actgcatgtt agtttgaaag accgtcccac agcagggaag gttcgaatcg
    71341 attatgatct gttcatggga ccggaaaaaa tcggcgaata ttatttgacc ttggatttta
    71401 cagcgtaaat ataaaagaaa agtttgattt tttgtgtctg attttgtatg ataaggagta
    71461 gactgtgaaa ggacgtgtcc tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg
    71521 aattatcaat gatcgaagta gcacacgcga tcttagaaca acgtggcgat gtcatggatt
    71581 tttctgattt agctaaccag atccaaaact atcttgggaa atcagacagt gaaattcgtg
    71641 attcactcgc acaattttat actgacttga atattgacgg cagctttatc tcattagggg
    71701 ataatcgttg gggcttgcgt tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc
    71761 atggaatgga cgaagaggat gaagatactc cacgtcgccg taaacgcaag aaagtcaacg
    71821 cctttatcaa tgatgatgaa gacgcgatcg attacaatga cgatgaccca gaagatacag
    71881 atctgacaga agaagatgac gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa
    71941 tcgctgctta taattctgac ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag
    72001 aagacttgcc aggaaatatc gaagaagatt taagtatcat agacgaagac gatgatgatg
    72061 aagattatga agaggaagaa ttttctgact ctgaaaaaga ataaacatta ttttcttacg
    72121 agaggttgtg acagagacga tcagcctatc agaataagga ggtgtgacac gatttttgtc
    72181 acaccttctt ttttgtatat aaaaatccca agtaaataca taaaaaataa aaaaattgaa
    72241 acttttagcg caccttgtac gtcatattaa gtgaggcaga aaggagtgga gaatgatttt
    72301 tactggatat aagaaaaaac ggcagatcga aaaataccgt cacctgctga atggactcat
    72361 cgaaactgat tatcaaaaaa tgttccgtat cacgatgaat tacgtacatg ataaagaaga
    72421 ggccttagat gtgatgcaag atagttttca taaagccctg aactattttg aaaaagaaaa
    72481 agagatcgag cattttagtg catggtttta tcggatattg atccgcacgg cattagatag
    72541 ttggcgcaga caaaaaagga cgcctgtgga cttatttgaa attcaagata tcccccaaca
    72601 gttaccttta gaaacttccg ttgcagagct ccatatgatt ttagataaaa tagacagtcc
    72661 ggaaaaagag attctgatcc tctacttttt tgaaggattt cgtttgaaag aaattgcgac
    72721 tatcttggat atgaatgaaa acacagttaa aacgaaaatg taccgctcat tgaatgcttt
    72781 acgtcaaatc ctagaatgaa ggagagaaga gtgccatgta ttcccaaaaa gaaaatttaa
    72841 aaaaactaaa gaaaacttat aaagaacaag caattcccaa agggcttaaa gaggagatcc
    72901 gccagcgttt tattatcgaa gagcaatcat ttataaaagc aaagcggaga aagagacgtc
    72961 tccaagggtt cggagtatct ttggctatcg taatgattac aacaagtagc cttcttttta
    73021 atagtcaagt tcgaagcttt gcagaagatt tacccatctt aggatcagtc attgaattga
    73081 tcttaggcga acgctttact gatcgatctg aaaaaatcga tattcaggta ccgaagatca
    73141 atacacaaaa tgagaaagaa aataaaacaa ttcatggatt aaatcaaaaa tactttcgcg
    73201 aaggacaagc agattttgaa aaagctgaaa aagaatatgg aaattttgaa acggatcatt
    73261 accaagtttt gggagattat cagaaaatcg tagacgataa ccggtttctc gtgattgaaa
    73321 ggcagatcac ccaaacagca gcagatagcc atgttgaaaa aagatacgac acaatcgaca
    73381 agaaaaacag tgtccagctc tcacttccac ttttattcaa agatgacacc tatctatctg
    73441 ttttgacaaa agaagtcaaa cggcagatgg ccgaacaagt caaggaagat ccatctaagt
    73501 actattggac tgagcaagat attcaagaag ggacgataga aaaaccaaca cttgttacgc
    73561 ctacacgaag tttttatttg aacaaagagc atcaattagt tttgactttt tctcaatacg
    73621 aaattgctcc tggttatatg ggtacaccag aatttgtcat tcctaagtcc gtgacaaaaa
    73681 cgattttggc atcggaagat tatttggatc attagacgag aagtagttgc gactagcagt
    73741 ttcaattttc aagtaagtac aagtaaataa taagaagaga aaaagagaaa gaagagatat
    73801 agtgaaaaga aattatttag ttatcagtat tattgcttta tccgtgcttt tgtttttcga
    73861 aattacttcg gtggcctcct tgatcaccga agtaaaagga caaacacact cttcggagca
    73921 ttcgtcacaa tctttcaaac aatcagaaca atcaaccatt aaaagccaat caactgaaac
    73981 agctaacgtc aaagaagcca ttcaagaatc agcggaaaag gtacaaagtc agtcatcaca
    74041 atcaacacaa gacagtacaa aaacgagccg agatccggct gtcaaaagcg tcgcaatcag
    74101 ctttgatgat ggaccaggag cgaccacaac accgcagctt ctgcgaatct taaaagaaaa
    74161 aaacgtgcat gccacttttt tcgtcttagg agaaaatacg gcgcagcatc ctgaaatcgt
    74221 gaagcagaca gctgaagcag ggcacgagat tggcaatcac acatacgatc atcagaatct
    74281 ggccactctt tctgcccagt cgatgacgga ggaagtgacg aaagcagaca ctgagatcaa
    74341 aaaagtcact ggcaaaaccc ccacttttgt tcgtccgccc tatggttctg tcacgaatgt
    74401 aggagcaacg atcatccagc gaccgatcat cgaatggtct gttgattcag aagattggaa
    74461 gacaagaaat cctgatttga ttttgcagaa gatccaagca acagtatacg atggggcaat
    74521 tattttattc catgatatct atcctgaaac gattcgtgcc gttcctcaag tgatcgatta
    74581 tctgaaagag caagggtatc gcatcacgac agttggggat ttattagggc atccaacggc
    74641 tgtcgagaac tattatggca gaaacgatca tcggccagtt cagtaagtga gacaagtatc
    74701 tttaataaat aacacatttt aataagtagt tgaacacata actaccttac ttgcaaaggc
    74761 ttaacaatat ctatacaggt acaacaaaaa actgtccacc aattttcttt ggcggacagt
    74821 tttcacttaa aaataaagaa gcaactattc gtaaattatg ctaaaatagc agtagaacgc
    74881 gtcctgaggg aatcgaaccc ccgtctcaag aaccggaatc ttacgtgata tccactacac
    74941 taaggacgca agcactggga taattgtaac taaacagata aaaaattaca aggggattgg
    75001 acaaaaaaat gaaatttgag caaaattttt cacaaaaaca gcagcagaca cagaaactag
    75061 ccatgacgca acagttgcaa cagtccatcc aagttttaca atataacagt gaggagctgt
    75121 tggcattcgt tgagaatcaa gcaatggaaa atccattggt ggaagttgtt gagccggaat
    75181 ggcagccaga ttacataaaa gcttcttcat cttcttatga aggagaagag acaaattacc
    75241 taaatcagat tcctgataca aaagggtctt tatttgattc attgatcgaa caagtccatt
    75301 tgaattaccg agatactttt ttgaggaaaa tcgttcttta tcttgtagaa tacatcgatt
    75361 tgaacggatt tttgacgatt agtttagaag aagccggaaa agagattgga gctacgccta
    75421 tccaaatgct cgatgcgttg actctgattc agcaattaga accagctgga gtaggtgcac
    75481 gtagtttgca agaatgtctc atgcttcaaa cagaacgaga tgattatgcg ccggaattag
    75541 cttatatcgt gttggaagaa tgttttgagg aactggtgga gcgaaaatgg aaagaaatcg
    75601 cacaacgttt ttctgttgat ctacatgctg ttcagcagat attcgactat ttacaaacgt
    75661 tgagtccaag ccctggccgg attttcgacc gtagcagcga attgtacatt attcctgatg
    75721 tccgggtatt agtggacgac gataaaaatg tacaagttat ctcaaaccga aaaaatcagc
    75781 caaatatacg ctttcaagag agttacttca aacaaatgtc acaacaagca gataaagaaa
    75841 ctgaaaatta tttaaaagaa cggaaacagg aattcgaatg gttgaagaaa acgatcctac
    75901 aacgtgggga tacaattttg cgggttgctc aagtcattgt ttcccggcaa aaagctttct
    75961 ttatcgacaa ggaacgtcca atcaagcctc ttacgttaaa ggaagttgca actgagatag
    76021 atgtgcatga atccacggtc agtcgtgcag tcaacgggaa atatttggaa acagacttcg
    76081 gtgtatttga attaaaaaaa tttttcacta cacgaattcc tacgaatagt acagaacaga
    76141 cagaagacct ttcagcagat acagcgaaga aaaagctgca ggaattagtc gatcaagaag
    76201 acaaaaacaa gccgttgtct gaccagaaat tggttgaatt gctgaaaaaa gatgagatag
    76261 cgatctcaag acgaacagta gcaaaatatc gtgatttatt aggaatcccg agttcatcaa
    76321 aaagaaaacg ttatgataac taatcagtca aaagactaga aaaagtattt cactttttct
    76381 agtcttttta gttgaaaaac gagagcaaaa ctgttagact tacagtcgtg aggttgagat
    76441 ttccctttat tgatgtgaaa ttcgaatagt taatttcttt ttttatcgta tatgggtcgt
    76501 tttaagtcta cattggacgt aaagtgtcca actaaggaga tcgctatgct ggatgaaata
    76561 aaaatgattg aatctgttgc accagatatt atcgaagtgt tgcaagaacg ctataaaatt
    76621 ttacgaaata tctattggat gcagccgatt ggacgtcgaa gtttatcgga aagtatgggg
    76681 atcacagaac gagtattgcg aacagaaaca gatcttttga aaaatctgaa tttgatcgac
    76741 acctcaaaaa gcggtatgac gctgacaact aagggcgaag aagtttatca aagccttgaa
    76801 aacttcatgg atcagctttt aggtacgcat caaaccgaac aacagttggc agaatatttt
    76861 ggtaaccaac gctgtatcgt tgtttcagga aacagcgaag agcagaccaa agtggctgat
    76921 gcattcggcg aagcgctgag cgaagcccta gaccgactac ttccagaagg cgagaatatc
    76981 atcgctgtga tgggaggaac tactatggct gtggtagccg aacaattgtc taatctggaa
    77041 aataagaaac ggcataacct gtttgtccct gcaagaggcg ggattggtga agcgataact
    77101 gtacaggcaa attctgtcag tgcaagaatg gctgctaaag caaacggtaa ccaccgtgca
    77161 ctttatgttc cggaacaatt aagtctggca acttacaatt ctttactcaa tgagccatct
    77221 atccaagaag tcttaaactt gattagtgaa gcgaattgtg ttattcatag tattgggcgt
    77281 gcattacaca tggcagcacg tcgtaagatg actgaaaaag aattagtcat gctgaaacaa
    77341 gcaaatgctg ttgcggaatc ttttggctac tttttcaatg aaaaaggtga agtcgtttat
    77401 aaggtcccta gaatcggcat agaattgcaa gatttggaaa aagtcccgat catcatggca
    77461 atcgcgggcg gaaaatcaaa agctaaagca attcgagctt atatgaaaaa tgcaccaaaa
    77521 caaacgtggc tcatcactga tgaagctgct gcaaatgaga ttttaaaagg ggtaaccctt
    77581 taaaataaat atttttttga ttttcataag gaggaaatct taaatgacag ttaaagtagg
    77641 tattaatggt tttggacgta ttggacgtct agcattccgt cgtatccaag atgtagatgg
    77701 aatcgaagta gtagcaatca acgacttgac agatgctaaa atgttggcac acttgttaaa
    77761 atacgacaca actcaaggac gtttcaacgg aacagttgaa gttcatgaag gttcattcaa
    77821 cgtaaacgga aaagaagtta aagtattagc taaccgcaac ccagaagaat taccatgggg
    77881 cgaactaggc gtagatatcg ttctagaatg tactggtttc ttcacttcta aatcagcagc
    77941 tgaaaaacat ttaacagctg gtgctaaacg cgttgttatc tctgctcctg gcggaaacga
    78001 tgttccaaca atcgtttata acacaaacca cgaaacatta actggtaaag aaacagttat
    78061 ctcaggtgct tcatgtacta ctaactgttt agctcctatg gctaaaacat tgaacgacaa
    78121 atttggtgtt gttgaaggat taatgacaac tatccacgct tacacaggtg accaaatgac
    78181 tctagacgga cctcatccta aaggtgactt ccgccgtgca cgcgctgctg ctgcaaacat
    78241 cgttcctaac tcaacaggtg ctgctaaagc tatcggttta gtaattcctg aattgaacgg
    78301 taaattagac ggagctgctc aacgtgttcc tgtaccaaca ggttcattaa cagaattagt
    78361 aacagttctt gaaaaagaag taactgttga cgaaatcaat gcagcaatga aagaagcttc
    78421 aaacgaatct tacggataca acacagacga aatcgtttct tcagatatcg ttggtatgac
    78481 ttacggttca ttatttgatg ctacacaaac taaagtaatg acagttggcg acaaacaatt
    78541 agttaaaact gttgcttggt acgacaacga aatgtcatac actgcacaat tagtacgtac
    78601 tttagaatac ttcgctaact tataagatag acaaacgtac aagtgtaaat tatagacatg
    78661 tacatttaaa agcggggaag caatcgcgct tcttccgctt tttttgataa taagtttatt
    78721 caggaggaaa taaaatggct aagaaaacag tgaaagatat cgatttaaaa gacaaaaaag
    78781 ttcttgtccg tgttgacttc aacgttcctt tgaaagacgg cgtgatcact gacgacactc
    78841 gtatcaaagc agcattgcca acaatcaact acgttctaga acaaggcgga aaagcaatcc
    78901 ttttctctca ccttggacgt gtaaaaacag aagaagataa agaaggaaaa tctttagctc
    78961 cagttgcaaa acgtctaggc gaattattag gtaaagaagt aacattcgta cctgaaactc
    79021 gcggagaaca attagaagaa gcaatccgca acatgaatga cggcgacgtt gtcgtattcg
    79081 aaaatacacg atttgaagat attgacggta aaaaagaaag caaaaatgat acagaactag
    79141 gtaaatactg ggcttcatta ggcgatgtat ttgttaacga tgctttcggt acagctcacc
    79201 gtgcacatgc ttctaacgta ggtatcgctt caactggtat cccaacagtt gctggattct
    79261 tgatggaaaa agaaatcaag ttcatcggtg aagcagtaga agaaccaaaa cgaccaatga
    79321 ttgcaatcct tggtggcgca aaagtttctg ataaaatcgg cgtaatcgaa aacttgattc
    79381 ctaaagcaga taaaatcttg atcggtggcg gaatgactta cacattctac gaagcaaaag
    79441 gaatcaagat cggtaactct ctagtagaag ctgataaagt ggaactagca aaagaattaa
    79501 tcgaaaaagc aggcgacaaa cttgtcttgc caatcgacaa tgtctgtgca ccagaatttt
    79561 caaatgatgt tgaaacacaa gtaatcgaag gagacattcc tgatggtttg atggctttag
    79621 acatcggccc agcatcagta aaattatttg ctgatacatt acaaggtgca aaaacagttg
    79681 tttggaacgg acctatgggt gtatttgaaa tgagtaactt tgctaaaggt acaatcggtg
    79741 tttgtgaagc aatcgctaac ttagaaggcg caacaacaat catcggtggc ggagactcag
    79801 ccgcagcagc agaacaatta ggatttgctg acaaattcac acacatctca acaggtggcg
    79861 gcgcaagctt ggaattgctt gaaggtaaag aattaccagg actagcagca atcaacgata
    79921 aataaacgac caagtttgct caattctgac gagtaatcga acaaaaggtg aagggagttt
    79981 tcatgactcc ctagccattt ttcaataaat taaggagtgt gttcctcaat gcgtaaacct
    80041 attatcgcag gtaactggaa aatgaacaaa actgcatcag aagcaaaaga atttgctgaa
    80101 gcagtaaaaa acaaaatccc ttcaaatgac aaagttgatt cagtcatcgg atctcctgca
    80161 ttattcttac aagaattaat gacatcagct gaaggtacag aactaaaaat ctcagcacaa
    80221 aactgctact gggaaaactc aggtgcgttc actggtgaaa cttcaccagc agctcttgct
    80281 gatctaggtg ttcattatgt gatcatcgga cattcagaac gtcgtgaata tttccatgaa
    80341 acagacgaag acatcaataa aaaagcgaaa gctatctttg caaatggtat gacaccaatc
    80401 ctatgctgtg gcgaatcttt agaaacctat gaagcaggca aaactgctga atggatcgaa
    80461 ggacaaatta cagctggttt agctgatctt tcagctgaac aggtgtcaaa catggttatt
    80521 gcttatgaac caatctgggc aatcggtact ggtaaatcag ctgacgcaca aatcgctgac
    80581 gaaatttgtg gagttgtacg tcaaacagtt gaaaaacttt atggaaaaga agtatcagaa
    80641 gcagttcgta tccaatacgg cggttctgta aaaccagaaa atattgctga atacatggca
    80701 aaagaaaatg ttgacggtgc tttagtcggt ggcgcaagct tgcaagcaga ttcattcttg
    80761 tcattgttag acgctgtaaa ataattgttg aaaatttgcg aaaaatgcaa gaaatgtttg
    80821 cactaatgag caaaattaac taaaatcgta tatgagggaa atcccttagg aaaaaacaaa
    80881 ctcaaaggag agacaaaaca tgtcaattat tactgatgtt tacgctcgcg agatcttaga
    80941 ctcacgtggt aacccaacaa tcgaagtaga agtatacaca gaaagcggag cttttggccg
    81001 cggaatggtt ccttcaggtg cttcaactgg tgaatacgaa gcagttgaat tacgtgacgg
    81061 agacaaagct cgttatggcg gtaaaggtgt aactaaagct gtcgacaacg taaacaacat
    81121 cattgctgaa gcaatcattg gctacgatgt tcgtgaccaa atggctatcg acaaagctat
    81181 gatcgcttta gacggaactc ctaacaaagg taaattaggt gcgaacgcta ttttaggtgt
    81241 ttctatcgct gttgcacgtg cagctgctga ctacctagaa gtacctttat accactacct
    81301 aggcggattc aacacaaaag tattgccaac accaatgatg aacatcatca acggcggatc
    81361 acatgctgat aactcaatcg acttccaaga attcatgatc atgcctgttg gcgctccaac
    81421 attcaaagaa gctctacgta tgggtgctga agtattccac gcattagctt caatcttgaa
    81481 agctcgcgga ttagcaactt ctgttggtga cgaaggtgga tttgctccaa accttggttc
    81541 aaacgaagaa ggttttgaag taatcatcga agcaatcgaa aaagctggct atgtacctgg
    81601 taaagacgtt gttcttgcta tggatgctgc ttcttcagaa ttctacgaca aagaaaaagg
    81661 tgtttacgta ctagctgatt caggcgaagg cgaaaaaaca actgacgaaa tgatcaaatt
    81721 ctacgaagaa ttagtttcta aatatccaat catctcaatc gaagacggat tagacgaaaa
    81781 cgactgggac ggattcaaga aattaactga cgtattaggc gacaaagttc aattagttgg
    81841 tgacgacttg ttcgtaacaa acactcaaaa attatctgaa ggtatcgaaa aaggaattgc
    81901 taactcaatc cttatcaaag tgaaccaaat cggtacatta acagaaactt tcgaagctat
    81961 cgaaatggct aaagaagctg gctacacagc agttgtatct caccgttctg gtgaaacaga
    82021 agattcaaca atctctgata tcgctgttgc aacaaacgct ggccaaatca aaactggttc
    82081 tctatcacgt actgaccgta ttgctaaata caaccaatta ctacgtatcg aagaccaact
    82141 tggtgaagtt gcagaataca aaggtttgaa atctttctac aacttaaaaa acaaataatt
    82201 cttttctaat gaagatacat gatcgactat tgaaagatag tcgagtatga tcacttagtt
    82261 aagtaatcga actctctact tgctgaaata tcagcaggta gagagtt
//
LOCUS       DAYYCQ010000003        77334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000003 DAYYCQ010000000
VERSION     DAYYCQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.003"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..830)
                     /locus_tag="ACHAYF_000225"
     CDS             complement(<1..830)
                     /locus_tag="ACHAYF_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM8577693.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
     gene            complement(901..1362)
                     /locus_tag="ACHAYF_000226"
     CDS             complement(901..1362)
                     /locus_tag="ACHAYF_000226"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8577694.1"
                     /translation="MFQILKLSKNEIRHSNFLAFLFAPNETHNLSDEFFKMFLKRYID
                     SNDDTKAAINYFDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQ
                     LNKYQIYIERNYSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
     gene            complement(1326..1451)
                     /locus_tag="ACHAYF_000227"
     CDS             complement(1326..1451)
                     /locus_tag="ACHAYF_000227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577695.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            complement(1654..2133)
                     /gene="rlmH"
                     /locus_tag="ACHAYF_000228"
     CDS             complement(1654..2133)
                     /gene="rlmH"
                     /locus_tag="ACHAYF_000228"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8577696.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2561..3850
                     /locus_tag="ACHAYF_000229"
     CDS             2561..3850
                     /locus_tag="ACHAYF_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8577697.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(3889..4494)
                     /gene="ytpR"
                     /locus_tag="ACHAYF_000230"
     CDS             complement(3889..4494)
                     /gene="ytpR"
                     /locus_tag="ACHAYF_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8577698.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4699..5163)
                     /locus_tag="ACHAYF_000231"
     CDS             complement(4699..5163)
                     /locus_tag="ACHAYF_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8577699.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5185..5505)
                     /locus_tag="ACHAYF_000232"
     CDS             complement(5185..5505)
                     /locus_tag="ACHAYF_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8577700.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5649..6728)
                     /gene="pepA"
                     /locus_tag="ACHAYF_000233"
     CDS             complement(5649..6728)
                     /gene="pepA"
                     /locus_tag="ACHAYF_000233"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8577701.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6928..7278
                     /locus_tag="ACHAYF_000234"
     CDS             6928..7278
                     /locus_tag="ACHAYF_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8577702.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7501..8448
                     /locus_tag="ACHAYF_000235"
     CDS             7501..8448
                     /locus_tag="ACHAYF_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8577703.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8505..9923)
                     /gene="pepV"
                     /locus_tag="ACHAYF_000236"
     CDS             complement(8505..9923)
                     /gene="pepV"
                     /locus_tag="ACHAYF_000236"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8577704.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10072..10917
                     /locus_tag="ACHAYF_000237"
     CDS             10072..10917
                     /locus_tag="ACHAYF_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8577705.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            10921..11361
                     /locus_tag="ACHAYF_000238"
     CDS             10921..11361
                     /locus_tag="ACHAYF_000238"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8577706.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11411..11605
                     /locus_tag="ACHAYF_000239"
     CDS             11411..11605
                     /locus_tag="ACHAYF_000239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577707.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            11741..12418
                     /locus_tag="ACHAYF_000240"
     CDS             11741..12418
                     /locus_tag="ACHAYF_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8577708.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(12826..13515)
                     /locus_tag="ACHAYF_000241"
     CDS             complement(12826..13515)
                     /locus_tag="ACHAYF_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577709.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13490..15619)
                     /locus_tag="ACHAYF_000242"
     CDS             complement(13490..15619)
                     /locus_tag="ACHAYF_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8577710.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15667..17004)
                     /locus_tag="ACHAYF_000243"
     CDS             complement(15667..17004)
                     /locus_tag="ACHAYF_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8577711.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17256..17615)
                     /gene="rplT"
                     /locus_tag="ACHAYF_000244"
     CDS             complement(17256..17615)
                     /gene="rplT"
                     /locus_tag="ACHAYF_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8577712.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17668..17868)
                     /gene="rpmI"
                     /locus_tag="ACHAYF_000245"
     CDS             complement(17668..17868)
                     /gene="rpmI"
                     /locus_tag="ACHAYF_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8577713.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(17940..18464)
                     /gene="infC"
                     /locus_tag="ACHAYF_000246"
     CDS             complement(17940..18464)
                     /gene="infC"
                     /locus_tag="ACHAYF_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8577714.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18508..18633)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            18784..19416
                     /locus_tag="ACHAYF_000247"
     CDS             18784..19416
                     /locus_tag="ACHAYF_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8577715.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19418..20323)
                     /locus_tag="ACHAYF_000248"
     CDS             complement(19418..20323)
                     /locus_tag="ACHAYF_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8577716.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20408..21067)
                     /locus_tag="ACHAYF_000249"
     CDS             complement(20408..21067)
                     /locus_tag="ACHAYF_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8577717.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21383..22327
                     /gene="mvk"
                     /locus_tag="ACHAYF_000250"
     CDS             21383..22327
                     /gene="mvk"
                     /locus_tag="ACHAYF_000250"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8577718.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22320..23297
                     /gene="mvaD"
                     /locus_tag="ACHAYF_000251"
     CDS             22320..23297
                     /gene="mvaD"
                     /locus_tag="ACHAYF_000251"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8577719.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23310..24395
                     /locus_tag="ACHAYF_000252"
     CDS             23310..24395
                     /locus_tag="ACHAYF_000252"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8577720.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24392..25456
                     /gene="fni"
                     /locus_tag="ACHAYF_000253"
     CDS             24392..25456
                     /gene="fni"
                     /locus_tag="ACHAYF_000253"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8577721.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25705..26508)
                     /locus_tag="ACHAYF_000254"
     CDS             complement(25705..26508)
                     /locus_tag="ACHAYF_000254"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8577722.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26528..27370)
                     /gene="kduI"
                     /locus_tag="ACHAYF_000255"
     CDS             complement(26528..27370)
                     /gene="kduI"
                     /locus_tag="ACHAYF_000255"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8577723.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27386..28393)
                     /locus_tag="ACHAYF_000256"
     CDS             complement(27386..28393)
                     /locus_tag="ACHAYF_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8577724.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28400..29044)
                     /locus_tag="ACHAYF_000257"
     CDS             complement(28400..29044)
                     /locus_tag="ACHAYF_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8577725.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29144..29902)
                     /locus_tag="ACHAYF_000258"
     CDS             complement(29144..29902)
                     /locus_tag="ACHAYF_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8577726.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30029..31180)
                     /gene="fucO"
                     /locus_tag="ACHAYF_000259"
     CDS             complement(30029..31180)
                     /gene="fucO"
                     /locus_tag="ACHAYF_000259"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8577727.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31262..31573)
                     /gene="rhaM"
                     /locus_tag="ACHAYF_000260"
     CDS             complement(31262..31573)
                     /gene="rhaM"
                     /locus_tag="ACHAYF_000260"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8577728.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31575..32420)
                     /gene="rhaD"
                     /locus_tag="ACHAYF_000261"
     CDS             complement(31575..32420)
                     /gene="rhaD"
                     /locus_tag="ACHAYF_000261"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8577729.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32434..33690)
                     /gene="rhaA"
                     /locus_tag="ACHAYF_000262"
     CDS             complement(32434..33690)
                     /gene="rhaA"
                     /locus_tag="ACHAYF_000262"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8577730.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33687..35147)
                     /gene="rhaB"
                     /locus_tag="ACHAYF_000263"
     CDS             complement(33687..35147)
                     /gene="rhaB"
                     /locus_tag="ACHAYF_000263"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8577731.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35300..36169
                     /locus_tag="ACHAYF_000264"
     CDS             35300..36169
                     /locus_tag="ACHAYF_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8577732.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36295..37194)
                     /locus_tag="ACHAYF_000265"
     CDS             complement(36295..37194)
                     /locus_tag="ACHAYF_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8577733.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37359..38183)
                     /locus_tag="ACHAYF_000266"
     CDS             complement(37359..38183)
                     /locus_tag="ACHAYF_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8577734.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38194..39489)
                     /locus_tag="ACHAYF_000267"
     CDS             complement(38194..39489)
                     /locus_tag="ACHAYF_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8577735.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39505..39984)
                     /locus_tag="ACHAYF_000268"
     CDS             complement(39505..39984)
                     /locus_tag="ACHAYF_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8577736.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(39990..41018)
                     /locus_tag="ACHAYF_000269"
     CDS             complement(39990..41018)
                     /locus_tag="ACHAYF_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8577737.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41035..42141)
                     /locus_tag="ACHAYF_000270"
     CDS             complement(41035..42141)
                     /locus_tag="ACHAYF_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8577738.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42370..44967)
                     /gene="adhE"
                     /locus_tag="ACHAYF_000271"
                     /gene_synonym="adhC"
     CDS             complement(42370..44967)
                     /gene="adhE"
                     /locus_tag="ACHAYF_000271"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8577739.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45272..45652)
                     /gene="yajC"
                     /locus_tag="ACHAYF_000272"
     CDS             complement(45272..45652)
                     /gene="yajC"
                     /locus_tag="ACHAYF_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8577740.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(45749..46894)
                     /gene="tgt"
                     /locus_tag="ACHAYF_000273"
     CDS             complement(45749..46894)
                     /gene="tgt"
                     /locus_tag="ACHAYF_000273"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8577741.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47112..47810
                     /locus_tag="ACHAYF_000274"
     CDS             47112..47810
                     /locus_tag="ACHAYF_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8577742.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(47978..50617)
                     /locus_tag="ACHAYF_000275"
     CDS             complement(47978..50617)
                     /locus_tag="ACHAYF_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8577743.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51236..52267)
                     /gene="queA"
                     /locus_tag="ACHAYF_000276"
     CDS             complement(51236..52267)
                     /gene="queA"
                     /locus_tag="ACHAYF_000276"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8577744.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52460..53635
                     /locus_tag="ACHAYF_000277"
     CDS             52460..53635
                     /locus_tag="ACHAYF_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8577745.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            53639..54280
                     /locus_tag="ACHAYF_000278"
     CDS             53639..54280
                     /locus_tag="ACHAYF_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8577746.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54277..55194
                     /locus_tag="ACHAYF_000279"
     CDS             54277..55194
                     /locus_tag="ACHAYF_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8577747.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55191..55862
                     /locus_tag="ACHAYF_000280"
     CDS             55191..55862
                     /locus_tag="ACHAYF_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8577748.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56403..56891)
                     /locus_tag="ACHAYF_000281"
     CDS             complement(56403..56891)
                     /locus_tag="ACHAYF_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8577749.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57092..57358)
                     /locus_tag="ACHAYF_000282"
     CDS             complement(57092..57358)
                     /locus_tag="ACHAYF_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8577750.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57358..57621)
                     /locus_tag="ACHAYF_000283"
     CDS             complement(57358..57621)
                     /locus_tag="ACHAYF_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8577751.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58142..58282
                     /locus_tag="ACHAYF_000284"
                     /pseudo
     CDS             58142..58282
                     /locus_tag="ACHAYF_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(58424..60247)
                     /locus_tag="ACHAYF_000285"
     CDS             complement(58424..60247)
                     /locus_tag="ACHAYF_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8577752.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(60845..63550)
                     /locus_tag="ACHAYF_000286"
     CDS             complement(60845..63550)
                     /locus_tag="ACHAYF_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8577753.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(63856..64803)
                     /locus_tag="ACHAYF_000287"
     CDS             complement(63856..64803)
                     /locus_tag="ACHAYF_000287"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8577754.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            64993..65262
                     /gene="rpsN"
                     /locus_tag="ACHAYF_000288"
     CDS             64993..65262
                     /gene="rpsN"
                     /locus_tag="ACHAYF_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8577755.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65438..65800)
                     /locus_tag="ACHAYF_000289"
     CDS             complement(65438..65800)
                     /locus_tag="ACHAYF_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8577756.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(65811..66932)
                     /gene="alr"
                     /locus_tag="ACHAYF_000290"
     CDS             complement(65811..66932)
                     /gene="alr"
                     /locus_tag="ACHAYF_000290"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8577757.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(66948..67298)
                     /gene="acpS"
                     /locus_tag="ACHAYF_000291"
     CDS             complement(66948..67298)
                     /gene="acpS"
                     /locus_tag="ACHAYF_000291"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8577758.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67428..68939)
                     /locus_tag="ACHAYF_000292"
     CDS             complement(67428..68939)
                     /locus_tag="ACHAYF_000292"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8577759.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69261..70613)
                     /gene="murF"
                     /locus_tag="ACHAYF_000293"
     CDS             complement(69261..70613)
                     /gene="murF"
                     /locus_tag="ACHAYF_000293"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8577760.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(70766..71842)
                     /locus_tag="ACHAYF_000294"
     CDS             complement(70766..71842)
                     /locus_tag="ACHAYF_000294"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8577761.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72038..73783
                     /locus_tag="ACHAYF_000295"
     CDS             72038..73783
                     /locus_tag="ACHAYF_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8577762.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74063..74500)
                     /locus_tag="ACHAYF_000296"
     CDS             complement(74063..74500)
                     /locus_tag="ACHAYF_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8577763.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74484..75857)
                     /locus_tag="ACHAYF_000297"
     CDS             complement(74484..75857)
                     /locus_tag="ACHAYF_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577764.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76179..76667)
                     /locus_tag="ACHAYF_000298"
     CDS             complement(76179..76667)
                     /locus_tag="ACHAYF_000298"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8577765.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(76689..77291)
                     /locus_tag="ACHAYF_000299"
     CDS             complement(76689..77291)
                     /locus_tag="ACHAYF_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8577766.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22220 a        16481 c        14093 g        24540 t
ORIGIN      
        1 taaactaatt tgaaatcgct acagatttct tgtcgatcac taacacgaac cttatgagcg
       61 atatttctag atatatggac acaacattgt tgaaattgag catttggata aacactatga
      121 atactatcgt caataccact taaaccatct gttacgacta acaaaacctc ttctaaacca
      181 cggtctttta gatcttgaag aatctctttc caaacataag cagattcagt tggagctata
      241 gtaaatccta gaacctcttt agttccatcc aacctgatac caattactat atagacagct
      301 tcttttgata cggtttgtct ttttaaggga atatgagtag catccataaa aatgactgag
      361 tatttagctt ctaaagttct ttctttaaat gccgaaacat cttcagacac aattttactc
      421 atatttgaaa tagtttgtgg tttgtaataa tgaccataca ttttttcgat tagatcagag
      481 atttcggaca tagtaattcc tttttgaact agctgaataa tagtggtttc taaagagtca
      541 ttggttcttt tataagcagg taaagtttgt tgagaaaagt ctccattcct atctctaggt
      601 attactaaat tcaattcacc gtactctgtt ttaaaagagc gcgaataatt cccattacgg
      661 gaattacctg aattaaatcc agctctatca tacttttcat agtcaagaaa taccgttaat
      721 tcagcttgta ataaagcgtt aatagccaat tctaggtgac gacgaaacaa atcatccaaa
      781 tcacctttat tgattagtat ttccataatt tctgtagtaa aatcgttcat atggaagtcc
      841 tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat
      901 ttacacaaaa tattttacac tctcttcaac gatattcttg taagtaatta tgccccaact
      961 aggatttgtg ggttcatttc catctggagt taaaaaaaca aaaatttttt tatagttaga
     1021 ataattgcgt tctatatata tttggtattt attaagttgc cctccagact ccgaagacag
     1081 aattttattt tcgatgcaca ccactatttt atttttttcc gataccaata aaatatcaat
     1141 attcttattt tctctgtaaa caattaaatc ttcatagcta ttaagtaaag catcaaaata
     1201 attgatagca gctttggtat catcattaga atctatatac cttttcaaaa acattttaaa
     1261 aaactcatca gataaattgt gggtttcatt tggagcaaac aaaaaagcta aaaaattact
     1321 atgtcttatt tcatttttag atagcttaag tatctgaaat atatttgttt tcccagtcca
     1381 tttagatagt tttcttcaat atcatccata tcctctaata gattttttaa acactttttt
     1441 tcatctatca tgttttttct ccttgccatt attctaacta aaattttacg tatttgtttc
     1501 tatgtctaca ttatcttttt aacagtatct atttttcttt tgcaacttac ttggctacca
     1561 aagcaaataa aaaagttatt tataaatttc tccctaccac tacctattgc tattatttaa
     1621 aatagggata attttttctc ttaaaaaata tcattatttg tggtatggag cccccgcatt
     1681 gatccggaag gcacggtaga tttgctcgac aaggatcaga cgcatcaatt gatgtggata
     1741 cgtcattttt ccaaaagaga tctgagcatt gcttcttttc atgacggctt cgctgagtcc
     1801 taaagagccg ccgataacaa aaacaaggtg gctttttcct tggataccta attggtcgat
     1861 ttgtttagca aaagattcac ttgtaggatt tttcccttca attgctaagg catagacgta
     1921 ttcttgttct ttgattttcg ctaagatccg ttccccttct ttttctttta cttgtctcat
     1981 ttgcgcttcg cttaaattct ccggggcttt ttcgtcagga acttcgatca attcgatttt
     2041 tgcataggca ttcagccgtt tgacatactc attgatacct tggatcaggt atttttcttt
     2101 taattttcct actgagataa ttttgatatt catgttttct ctcctttggt cttcatgctc
     2161 actttgttta ttataatcaa ttttttcgtc tatatgttta ttttggctat ttttgttaaa
     2221 aagatttccc caacagttat gcacaacatc cacaggttta tccacagaaa acagtgttat
     2281 tttaccttat ttcatcacat tcattaggca aacatacgtt ttataagaat ttatccactt
     2341 tatacacagg ctgtggatat gttttatccc ttatttttcc aatcctatcc gcgtagtttt
     2401 acacagtatc tgtggaaaaa gaaaatcaaa tattttcatc tgaaaacaat gatttttcct
     2461 aaagtttttc ataaaaagtt cacagtctca aagtagacta aagtcaacaa ataagactat
     2521 actaacagta gataaatgaa tgtaattgga ggaggaacac atggatagaa aaaatgtgac
     2581 accaaagatg aaaaaaagta aaaataatag cctctggcgt aagttaggtc tggggcttgt
     2641 tggagggatc gttggaggac tactgacagc tggcattttt tatgcagcga tgggcactgg
     2701 caacaatagt tctactgcta caagcggcaa tcagaattct gccggtgaaa cagttgtgga
     2761 aaatgtcaaa gtcaatgtag attctgatat caccaaagcg gtagataaag ttcaagacgc
     2821 tgtcgtttct gttatcaact tacaaagtca aaaccaatca agcggttttg gcggactatt
     2881 cgggcagcaa gaagaaagta gcagctcaga tgatagtaat ttggaagctt atagtgaagg
     2941 aagcggcgtg atctacaaaa aagacgggaa tactgcttat gtagtcacga ataatcacgt
     3001 agtagatggc cagcaaggtc ttgaagtctt gatgaaagac ggtacgaagg taaaagctga
     3061 attagttgga acagatgctt attcagattt agcggtgttg aagatcaatt cagacaaagt
     3121 cgaaacagtt gcctcttttg gtgactcaag tgctctgaag gttggtgagc ctgcgattgc
     3181 gattggttcc ccactaggat ctgaatatgc aaattctgtc acttcaggaa tcatttcttc
     3241 cttgaaccga caagtgacaa gtacgaatga atcgaacgaa acagtcaata tcaatgcgat
     3301 ccaaacagat gcagcaatca accctggaaa ttctggtggt cctttagtta atatcgaagg
     3361 acaagttatc ggtatcaact caagtaagat cgcaagtact tctgaatcta cttctaatgt
     3421 aagcgtagaa ggaatgggat ttgctattcc aagtaacgat gtagtgaata tcatcaatca
     3481 actggaaaaa gacggaaaag tgacacgtcc agctctaggt atcacaatgg tcgacttatc
     3541 tgccgtttct actcagcaac aagaacaaat cttgaagatt ccttcatctg taactaacgg
     3601 tgtcatcgtg acaaatgtcc aaacagctac accggctgaa aaagctggat tgaaacaata
     3661 cgacgtcatt actaagatcg atgacaaaga agtttcttct ggtgtagaac ttcaatctgc
     3721 tttgtataag aaaaaagtgg gagattctgt caaaatcact tactatgaag gctcgaaaga
     3781 aaaaacaact actgttgaat tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag
     3841 tgaaaattaa taaaaaagag gttgtgacaa agacttgtca caacctcttt attttggaaa
     3901 tgcttctccg actacggcat cctcaggcaa tactaagatt cctttttttg ctggagcatt
     3961 tggcaaatgc aattcacgag cggaacagat cattccatag ctatctaccc ctcgcaaagt
     4021 tcctggccag atcatcattc catctggcat catagctcct ggttttgcaa cgacaacttt
     4081 ttgtccagct tcgatattgg gtgcaccgca aacgatttgc aatactgtac cattgtctac
     4141 ttctgtctca gtaatcgata aatggtcaga atcaggatgt gggacgcatg tttttacgta
     4201 cccgacaacg atcttcggtt catgatcagc ttcaagctgt tcttcgaatc ctgcttcttt
     4261 gatcaatcga ttcaatttcg ctaattcctc atctgttaag cttacttggc cgttcccttc
     4321 gattgttaga tgatcggaga tatggaaaaa gttccaagca acaacttgac cttgttcatc
     4381 tttgatccga gcaacattcc atttgcgttc aacagtgttt tctgttcctt tatcgtcggc
     4441 tataataacc atcaaggtgt ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt
     4501 tcctccatta tcctttgctg agatttatct taacaaaaaa ttgcattcac tgcatgaaat
     4561 ttttttcatg ctttatttcc gaagaagcct cttctggaac accatttatt cgtaacaagg
     4621 gactgtgaca agacttttgt cacagtccct ttactgtttt agttaattga ttaaaaaggt
     4681 cgtacaagtt ccacttgctt aattacttgg ctgttcttct tcgtcagaaa cgatcaagac
     4741 atcacaaggt gaatgacgta tcacgtagct ggctacactt cctgtcataa agcgttcgac
     4801 tgcatttaga cctgactgcc caaccatgat tagatcgatc tcatattttt ttggcaattt
     4861 gactgccatg gattcacgaa cagaaccata tgcaataatt ccttctatat ttgtgtaatc
     4921 aacagatttc ccatattctt tacattcttc gatcatttcc ttggctgttt cagtttgctg
     4981 atcgactaga ttttgatcaa taggtgcata gcccataaag ttataaaatt gctgatcgat
     5041 tacgtttgct acatatactt tcccattatt acggcgagcc acttctaccg cttttttgaa
     5101 agcagcaagt gcttgttcac taccgtcgat acctactaaa atattcttat attcttgatt
     5161 catcttgacc cctcctaaca gatgtcagcc ttttgttaca ctttctagga acgattcgat
     5221 ttcctgtttt gttttacgat ctttattcac tagacgacct aattcttttc cgtcttggat
     5281 cacgacaaaa cttgggatac caaaaatgtt ccattcagcg gctacatcta tgtacttgtc
     5341 acggtctact tcaataaatt gccaatcaga aaagtcattt tcaatttcag gcatttgcgg
     5401 tttgataaaa cggcagtctc cgcaccagcc ggcagtaaag aaaaatacac tctttccagt
     5461 ttctacgtag cctgctaatt cttctaaact atttgggata atcattcttt gtcctctact
     5521 ttctgtttat gtatttttca attaaattat acacctttct ttcgaactaa caaacattga
     5581 tcctcttttt ataaaaaaaa tctggcaaaa agcaatcttt tgccagattt tatagtttca
     5641 ctatcgcatt attttccgta tacgatcgta tttactgttg atttatccaa acctttcagt
     5701 gtttgaatca acatttctcg tgcagcttca aaatcgcgaa tactgaacat cgtttgatgc
     5761 gtatggatat aacgtcccac tacgccaatc actgtacttg ggattccttc gttttgtgta
     5821 tgagcagcac ctgcatctgt accgcctttt gaaacaaagt attgataagg gatattgttt
     5881 gtttcagcga tatccagcaa gtactcgcgc aaacgaggaa gcatgatcaa acctggatct
     5941 tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg tgccattttt cgtcactgta
     6001 tcatctgctg cagagcagtc tactgcaaag aacagatctg gcttgaattt attgacagaa
     6061 gctttagaac cacgcaagcc tacttcttcc tggacattcg cgcctgcaat caatgtatga
     6121 cctaatgttt cattttgcaa cgcttctaaa gcttctaata ccattgtaca gccatagcgg
     6181 ttgtcccatg atttactgat aatattcttt ccgttggctg ttttgatcgt ttcagcgtaa
     6241 ggaacgattg tgtctcctgg aaggacaccg taactcattg cctcttctct tgattcaaac
     6301 ccagcatcga acaaaatatc ggttacttct actgattttt gtccgcttgt tcctcgcaat
     6361 aaatgtgggg gaacggaaga agaaatacat ggataattct taccagtaga ggttttaagt
     6421 gtaaaacgct gagcagaaac aacgtaagga ttccagccgc ctagaggtac gactttaaaa
     6481 agcccgtttt cttggatttg tgtcagcatg aatccgactt catccatatg tgctgcgacc
     6541 attacacggg gtgcatccgc ttcttcatga tggcgcagac caaaaatgcc gcccaaacca
     6601 tctaactgta actcatctac cagcggttcc atatgacttt ccatataagc gcgaacatct
     6661 tgttcaaaac cgcttgttcc ttgtaattct gttaactctt tgattcgttg aaatgttttt
     6721 tcttccatat gctattcgtc taaatttcag actaaactcc tctcagactt atattgatct
     6781 gcggataaaa tatgattttt tctgcagacc gatcgtattt attatagctt attttttctg
     6841 gtagcggtta tttatttcaa agaaaatatg agagtgtggt aaaataagca caaggagtaa
     6901 caagtgagaa gaaggaggta tgtccacatg tatgaagaaa gcgaactaag ctattttaaa
     6961 ggcgggttag cagttggaat cggtctagga ttattgagcg gggtagcttc aacgatttgg
     7021 tatcatcgca aaaaagtatt gtcagcagac caagtacttg aaaatatcaa gtcatctttt
     7081 ctaaaagaag gtgcaatcga aggttcttgg atctcttttg aaaaggaacc tattaggaaa
     7141 ttcgccgttc ggtcgagtgc ttatcgggga ggtctttctc gaatagaaga tggcgaaact
     7201 gtttattatg aatttttagc agatgctcat acaggaaccg tattagaaat ttcaagaaaa
     7261 aaaggtgcaa cagattaata cgtcaaaaaa gtgagagaac gaatacgcac ccactattca
     7321 aaagagaggt atcatgtcct tcttttgtag tgggtggttt tttagataga gctttccaaa
     7381 catatattcc agtattttct ttttaaataa atgatattta gcgggaaaaa ctagcatttc
     7441 ctttgattat gaggtatttt taatttagct tttgttttct atagttaagg agagtgaaaa
     7501 atggaacaac aagaaaaaat tgctgttctg attccttgtt acaatgagga agcaacaatt
     7561 tcaacagtca ttgcagactt taaacgagaa ttaccagaag cagatattta tgtctatgat
     7621 aataattcga ccgatcgaac atacgaattg gccgttcaag gtggagcaat cgtaaaaaaa
     7681 gaacctcgtc aaggaaaagg aaatgtgatc cgccaaatgt tctttgatat cgatgccgac
     7741 tattatttaa tggtagatgg cgatgatact tatcctgcag aagctgtaca tggtttgctc
     7801 gagaaattac gttctggtga agcagatatg gtcattggtg atcgcctatc aaatggaacc
     7861 tactttgatg agaataagag acctttccac gattttggaa ataaccttgt tagaaataca
     7921 attactagaa tgtataaaac aaaaattctt gatgtcatga ccggctatcg aggattcaat
     7981 cgtatctttg tcaaaagttt tcctattatg agttctggtt ttcaaatcga aacggaattg
     8041 acgatccatg ctttagataa aaaattcaag ttcgttgaaa tcccaatcga ttatcgtgat
     8101 cgtcctgaag gcagcgaatc aaaattgaat acattctctg acggattcaa agtgatcatg
     8161 atgattatta aaatgtttaa agattataag cctttgcttt tctttgggat ttggaccatc
     8221 ttcttctttt tatttggatt ggtcacgggg attcctgtga ttcgcgaata tatgctgaca
     8281 agctttatta cacgcatccc ttctgcgatt ttaagtactg gattgatgac tttagcactt
     8341 ctttcattag taacagggtt gattttggat acagtcgtca cgaacgctaa gaaagaatat
     8401 gaattgaatc tatatcatgt ttatcatgaa taccacagta gaaagtaaaa aaaggatcaa
     8461 gactgcaaaa ataatttgca attcttgatc ctctttttaa ttaattattt cgccaaacga
     8521 tagatggcat ctgcatagat tgctgttgct ctgaacaagt catcaagact cataaattcg
     8581 tttgcttgat gcattgtatc agtatatcct gggaacatcg ctccataagc aacgccacgt
     8641 ttcaacaaac gtccgtaagt tccgccaccg atgatttgtt cgtagccttt ttctccagtg
     8701 tggtcttcat agacttgcaa taatgtagcc accaatggat catccattgg tacgtaatgt
     8761 ggttccatta cgcgagcacc tcgagtaact gttgcgcctt cttgccccac tgtttgttcg
     8821 atgtcacttt gcaatccgtc tactgttaca ccttttggaa aacggaaatt catattgatg
     8881 aagttcgctt cctctgtacc attttcttca aaagcaaata gtccagcatt cattgtcaat
     8941 ttgcccattt tttcatcttc ataagcgact cctagttttt ctccataaaa atcttcatgg
     9001 acataagcag ctgctacatg gataaattgt ttagcagatc ctaggaattc atagttatct
     9061 aagaacaatg ccaagaaaga acctgcattg ataccagatt gtgggcttgc accatgtgcg
     9121 cctttaccaa ctaattcgat ttttacatac gtattgtccg cttcaatcgt tccgcttact
     9181 ggatttgctt cgatgaattg gtaaaatgct tcttccatcg cgatagctgc atctgcactt
     9241 ggtacttgaa gtgctgcagt tgctgttcct ggcaccatat tttcgcgaag tccggaaaca
     9301 aaagatttca agacataatc tcctgcattg tcgcctttga agcgtaaagc taaagaaaca
     9361 tttccttttt caccattgat gattgggaat tcagcatctg gtgagaaacc aaaatctggt
     9421 tcttcttcat gttcgaaata ataagccata tcgccccagc cgctttcttc atcgctgccg
     9481 actacgaagc gaactttttt tgatagtttc aagtcaagtt ctttaatgat cttcatcgca
     9541 tagtaagctg ccatacttgg acctttgtca tcgcttgatc cgcgtgcaaa gatctttcca
     9601 tccttgatta ctggttcata aggatctgtg tcccagccat ctcctgcagg aacgacatcc
     9661 atgtgtccaa atatccctag cgtttcgtct ccttcgccaa gatcaatatg ccctgcatag
     9721 ttatcgacat tttttacttt aaatccatca cgttcgccat aagctaacat atgtttcaat
     9781 gcgtcgcgag gtccaggtcc aaatggtgca tctggtgtta cttttgagtc atcgcgctcg
     9841 ctgttgacac gtaaaagatt tttcaagtct tcaagcaagt cttctttacg tgcttctact
     9901 tctttttgcc aatcaattgt cataaagtta atcctccact tatttaagtc ttcgtgttca
     9961 tgataccaca aattacagaa aaaaatagtt caaaaaatct gacaattcct tttcttatct
    10021 gtttttctct aaaaaaaata tacgctataa tgagtaaaag gaggttttta gatgctgata
    10081 aacgaacaac tagtgaaaag agtcatcaca cctcgtccaa aaatttcaca taaaggaaca
    10141 tttggacgta tcgttttgat tggcggcaat gaacaatatg gcggagcaat cattatgagt
    10201 gctttagctg ccgtacacag cggagcgggt ctcacgactg tagtaactga tgaaaaaaat
    10261 cggagtgctc ttcacagtca tctgccagaa gcaatgattg tagtttggac ggatactgac
    10321 cagtttcttt ccgtaataaa gcaagcagat gtactcttga ttggtcccgg attgggtact
    10381 tcttctaaga gtttggaacg tttcaaacaa gtactagaaa accaatcaaa agaacaatgg
    10441 ctggtgattg atggctctgc tttaagttta cttgctgcta atcatctttt gctaccctat
    10501 cccgaacaaa ctgtacttac acctcatcaa atggagtggc aacggttgag ttctctaccg
    10561 atacaagaac aacgggataa agaaaacaaa aagaagcaac aggaaatcgg tgctgtgctc
    10621 gttttgaaaa gtcatgagac aaaaatttat ggtcaccagc acttctacac caatccttta
    10681 ggttctgctg ctatggcaac gggcggcatg ggtgacactt tagctgggat gatcacagga
    10741 tttttagccc agtttcctca aaatgaccaa acgattgcag cagctattta tcttcatagt
    10801 ttcattggag accagctggc aaaagaacaa tatattgttt tgccgacaca aatcagcgaa
    10861 gcacttccga aatggatgaa ttatttttgt aaaaaatcaa tggaaaacga ggcatgagtc
    10921 atggagaaat ttacaggtta tgtgcgagaa cctttttatt atgaaacaga tcaaatgggg
    10981 attattcatc acagcaatta tatccgctgg tttgaagagg ctcgggtagc cttattggta
    11041 catctagggt ttccctataa aaaaatcgaa gaatctggga tcatcatccc cgttctggaa
    11101 gtaaattgca gctataaaga aatgatccat tacggagaaa aagtgagcat ccatccaact
    11161 atccaaaagt atacggggac acggttggat tttagctacg aaattgtggg aactgaagac
    11221 aaaaaattaa aaactactgg cagtagtaaa cactgttttt tactttctga ttcgcaacga
    11281 ttagtgaagc tgagcaaagt caatccggag ttagatcagt tattccaagc gtacgcttct
    11341 ttggatgatg taaattctta ataaagaccg tctaagcatt tttcttgtat tgtgtcgagt
    11401 agttcgtata atgaaaagaa aaaagcaggt gatccccatg tatgtcgtta aagtaatgca
    11461 cggttacatc gataaaactg gctgtcgcac tcgggaaaaa aacttagaca atctgctcat
    11521 tttcaaagac aaaaaagaat ccgaagcttt tgctaaaaga atcggcggtc gtgtcaaacc
    11581 gatccaagag gtacgaccag attgattcaa tatttttctt ttcgaggctc taataggcat
    11641 gcctatcagg gctttttttg cagactgtgt ttttatgccc acacgatttg taagcgattt
    11701 atgttataat agattatatc tgcatagaaa ggaaggttaa atggttatca ataatttgca
    11761 acagcaagct tatgaatcta tacgtaaaca aatcatttat tccgagttag cacctggaag
    11821 taaaatctcc gataaagtgt tagaagaaca actagctatc gggcgtacac cgattcgaga
    11881 agcactgatc caactgcgca atcaagagct tgtaaaaacc attccacagt ccggcactta
    11941 tgtttcaaaa atcgatatcc gatctgcttc attagcccgt tatacgcggg aaaagttgga
    12001 aaaaccgatc ctgcaagaat gcagtgcgaa aatgaccaag caagatcaag ctaaactaga
    12061 aaaaatacta gaacaaaccg atcaggcagt aatcgctcaa aataaaaaag ctttttttga
    12121 tttggatact gattttcacc gaacttgtta tgaaattgct ggaaaacgag aaatttggga
    12181 ttggctagaa agttatagta cgcgtttcag atggttacgt ttgacgattt cagaattaga
    12241 ctggggtcga gtattagatg aacatcagac aatgcttcaa tcaatgattg atcataactt
    12301 tgatgaagtc ggatttcttt gcacgatgca tttacatatg attatcgagg agcaagaata
    12361 cgtgattcat aattaccccg attattttga agatattcag gacagaccca tcaaataaga
    12421 ggttttggaa tcagcgtttt gacctgagta ttagagaaag aaacggcaaa atagttcctc
    12481 atattttgcc gttttttttg agctaatacc gagggtcagc tgattgcaca ccgtttattg
    12541 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaatagctt
    12601 cttatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgtttt
    12661 ccaagaggtc gtgagcctgc cgttcatctc cgagtcacaa ggaacaattt caaaaaacag
    12721 cttctcatgt ttgttgaata ttttgacttg tgaccgagga gttgacagga gaacatcgtt
    12781 tattcggaaa aaaaggactg tgacacgact tttgtcacag tctctttatt tgtaatctaa
    12841 atcatactcc gtacatcttt tttctatttt ttttatttct tctaagatcc cttggctacg
    12901 aatttcatta tatggccaaa gtaagactgc ctctaataca taaggcgaaa aatcgctttg
    12961 attcatctct tttgaaaaac tcatcgctgc tatctcgctc aatgcacgaa tgtttgattg
    13021 atgtgtataa aaaggcaacc gccacagcac aattttttcg ctttgcgtat aaattccctt
    13081 aacagaagct tcgatgtggt gaaatagttc atgtccaata atcaaagctt gcgctgtttc
    13141 tttagagatc gaagctagtc ctgaacattt taatttttct aacggctccg tcatcaatcg
    13201 aatcccttca tcttctgcat aagttgcaaa agtgattcgt tcgtttattt gctctgattc
    13261 acaggaaacg attggaacag ccatttgttc acaaatttct cctacatcgg caaaagggta
    13321 cttttttttg atgctacatg ccgtttcgat accacaatcg attgattgtt gtataagaat
    13381 tttctgcttg gacaaaggaa tctttttcca aaataaatct cgccgaaact ggtagaatcc
    13441 ccaaatttct tcggggatct gttgtaattc gtttagtaac tcttctaact tatgtttgtt
    13501 tgatatttcc ggcaacgacg atcacttctt cctccggttc catagtagat acttccagat
    13561 ccatcaaaag ctgtagttgt tccagatccg tcgctgtaaa atcagagact cttgctccta
    13621 agcagagata aaattctggt cgggcaatca attcagtttg ataaactgcc atatcttccc
    13681 aaagttgttc gacagctgtc atataattgg ctgcagttgt tttcatacat gaagcatgtc
    13741 cacgttggac cttgataaat cctttttgat cgatgatccg aagacttcca gcattttttg
    13801 aacgattctg ttcgccgcat acataaaaga aaggtgtatt ttccaataag caaacttcat
    13861 ttttatttaa tcgcatatcc tctgcagcaa gctctagtgc ttcttctgtt gtgatctcct
    13921 tggttagatc ggttgctttc acttccgtag aaccagtagc gatagccgtt acttttgaag
    13981 tctgtggatc gatttctacg tggacttcga tcgattctgg tgtagcgcct gattcaatcg
    14041 ctttattcat cgcttcattt ttcaacgatc gtatatcttc tttacttggc gaagggatga
    14101 tacgttctac aacatctcgg accattgcaa gtgctacacc aatagaagaa atcacttcgg
    14161 cgttttcagg aatcgaatat ttcacaccca ttttattact aaagtaagta atcaatgagg
    14221 cagcgccgcc ccctacacct acaagagaaa tctgatcttt ctccaagtga tacttgtctg
    14281 ccaattctaa aatcaccggt tcaatcttag cataggattt ttccatgatc tgttctgcga
    14341 tgtcttcaac cgtcgtatga caataatcag ctaatgcttg gatggctttt cgtgcagatg
    14401 ggacatttcc ataagaaaaa tgttcttctt gcactaatcc aaggacattg gctgcacatg
    14461 tatttgtaat agtcacttct tctccgtctt ccatcactac cttgacgtaa tcagcagggt
    14521 ctccttcttt aggagagaaa aattcaacct taggaccttt gattttttct gtctccgtaa
    14581 atactgcata atctagaccg gcaatatgtg cagaacgtgg accgacatcg atgattcctg
    14641 actgattagc ccgtaccatc gaaccgcctg ccacacctag aacccgaaca tctaatgaag
    14701 agatatatgt tgggtggcca cctacaattg aataatcgat tgctggacgt ccatttttaa
    14761 tgaccccgat attggttgtt gttccgccta cttcaaaata aacaccatta gacgctctca
    14821 agtacatcaa agagcccatg actgatgctg ctggaccgga aagcatggtt aaaacaggtc
    14881 gttttttcat ttcattgatt tccattactc cgccgtctcc gcgcataatc atcaaagaaa
    14941 cattgacccc tgcttctcgt acggaatctt ctgtagaggt tgccgtatct agcatttttg
    15001 gtaaaataga tgcattgatt gctgctgtcc gagttcttct tgtcaatcca tataattttg
    15061 tgatgtcaga agccatcgtt gtagggatcg attttaccga tgctgcttca taaacaactc
    15121 gttctggttc cccattatct actccaaatg ccatagaaga aaccaagacc tccgctcttt
    15181 ctctttctaa agaagaaatc gtttctgaaa cacggtctac atttaagtgt ttgacaggta
    15241 agaatgcatt gacaatctct atttttttct tattccctaa atcgatatcg ttcaatctag
    15301 tttgtcgttt tgctaaaaaa ccttctaatc caccttttgc catcccgatt acgccgactt
    15361 ttgctacgtc tccttcaatc agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca
    15421 caacatcttc tggactgata ttattttcca gcaaacaatt ttgaaaagac tgaacgacac
    15481 ctgcggctac tccacggaca tcatcgtggg ttgttttcac tgaagatttt ccgatgattt
    15541 cgtgcgtcgc attatcgatt gcgactgctt tcgtatgtgt accgccaaca tcaattccca
    15601 ttcgcacaaa acgtttcatt ttcttttcct ccattcatct aatcgttttt atcgacgttt
    15661 tgtttactag gcgttcccgt acaataagaa cgtgatcagg cataagatcg ctccgatgat
    15721 ccaaccagtt gggatggatt tcttcatata ttctcgagaa gttacttttg tataacctaa
    15781 tccccaagca acccaagatt gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc
    15841 aaataacggg aacaaaaagg ctggcgacat cgttggatta acagctaata cgacagctaa
    15901 aatagctgag cctgaaccga ctagattcat tggaccgcgg aaaaatccta gtggtgtcaa
    15961 tatagcaaat acgattgcca agactaatgg tgattgcggt atccaatctc caaaaatagc
    16021 tgaaaaataa ggagaagcat acgcagctga attattgaac atcgctaagg tcatcaagaa
    16081 accaaccatt ggcgcaacat ctactgcgcc atctgtaaat aatttcgcaa actgacgaca
    16141 gatttcttta taacctccgc gcaattttcc agtaaacagc aatgcccaga tacctgctag
    16201 aataaaacct aagatgattg gaagatctaa aagaacgacc cctaaaactg gtaataatac
    16261 ggctaaccat gagatcattg gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc
    16321 atagcgtttc tttttgttca ttgatatatt agcgaccgtt agtaccacaa ctaagcttac
    16381 aatcatccca atcataccaa tttggaaata gtcattataa gtatagcttt cagccgctgg
    16441 attgaaacca gcataaatcg tttgatattg tttgaaattg acgatattag caaagattcc
    16501 agccataatc gagcccataa atgagaacag tgcaaccggt actgaaatac ctaatgacat
    16561 caagattggc aacacaataa ctgcaataga aatcactggt ccgattcctg tcatcgacat
    16621 gaatataaca gcggttacaa tacatagtaa gctcatcgtg attcttggct tgtcccctcc
    16681 aagttcaact acctttcgaa tcagggtagc tgcgattccg ctatctacta gaacacgccc
    16741 gaaaaaggca ccaaagaaga catttaccaa gatcgatttg gcgtactcag ctggccccgt
    16801 cgtatagata tgcgtcaaca catcaattac tccttgccct tccatcgccg ggtttggtga
    16861 aaacgtgttt ccgactagcg ctaatcccat ccatacagta gccatgatgg caaagccgac
    16921 catcaagttg tagcctttta cacagtacca gaccatgaaa aagaatgaga taactaacaa
    16981 aataccaata ataatatcac tcatgttatt cctccgtcca ttttgttttt ctgataagca
    17041 aactaatata tcagtcaaac tcattctaac ttatgtagtt agcgctttca ttaaagggtg
    17101 accataaatg ttcagttttc aattaaaaaa ccttaattat gctattttag acttatacgt
    17161 tattgtcctg tttcatttga tggaggattg taaataaaaa aagaggaaag aacataactt
    17221 ttcgttatct tctttcctct ttataattgt ttagcttatt tagccaaagc ttcttttgct
    17281 tggtcagcca atgcagtaaa tgctgctgca tcgttaacag ccaagtcagc taacatttta
    17341 cggttgatgt cgatttcagc aactttcaaa ccgtgcatta attttgagta gctcaagcca
    17401 ttcatacgag ctgctgcgtt gatacgagca atccacaatt tacggaagtc gcgtttcttt
    17461 tgacgacgat cgcgatatgc gtagttgtat gaattcatta cttgttcttt tgctgtttta
    17521 aataaagtgt gttttgaacc gtagtaacct tttgctaatt ttagcactct tttacgacgt
    17581 ttacgagtta ctgttccacc tttaacacgt gccatgtata attcctccta ataaaaataa
    17641 atcttcagat tctgttattt gtacttctta tttcattttt gctagttgtt ggcgaatacg
    17701 tttgaagtcg ccactagaaa ccatgcttgc tttacgcaat tgacggcgtt gtttttttgt
    17761 tttgccgtgg aaacggtgac ttgtaaacgc gcggaagcgt ttcaatccgc ctttaccagt
    17821 acgtttgaaa cgttttgctg atccgcggtg tgttttttgt tttggcatga ctatatttcc
    17881 tcctcaaaat ctttcgttat ccgactatcg gctgtccaac agccagttag tcattcaaat
    17941 tacttactgt cgtttttcgg tgccagcgtc aagaacatgc tgcgtccgtc cattttcgct
    18001 ttttgttcca ctgttgcaat atccgcagtt tcttcagcta agcgattcaa gactttttga
    18061 ccaatttctt tatgggtaat ggcacggcct ttgaaacgga tagaagcttt cactttatcg
    18121 cccttttcca agaacttacg tgcattacga agttttgtat tgaagtcatt caagtcgatc
    18181 gtaggactta agcgaacttc ttttacattg atcacttttt gttttttacg cgcttcgcgg
    18241 tctttctttt gttgttcgaa acggtattta ccgtagtcca tgattcgcgc aactggtggt
    18301 ttcgcaccag gggcaactaa cacaagatcc aagtttgctt gttctgcaat attcaatgct
    18361 tctgcttttg tcttaactcc taattgctca ccatctgaac cgatcaatcg taactctcgt
    18421 gcacgaatgc cgtcgttaac catcatatcc tttgctatgg tcattcacct ccaaatatta
    18481 tgagagaaag aaaagcttac cgaataaacg gcaataaaaa aacgagttct cttacagaac
    18541 ccgcgcgtac ttcacctaat agaaccagtg aaaactatct agcccagtga cattatcaac
    18601 taaggcgaga agcgggtgct tctgctttta tttctcaacc atcatagtat agcaaaaact
    18661 aatctttttg tcaatgtttt tcatacagtt tgtcttcctt ttctgaaaat gattagatta
    18721 gcagactttc tagcagtgat tcccgcttct ttgctatact aaagaaaaac gaggtgaacc
    18781 cacatgaaaa aaatcgaacc tattccacct tctcttcctc ttcttacaaa gggagagttt
    18841 tatttagaag atgaggagat cttctctggt cttctgatca acgggggtga ccaaagctat
    18901 ctcgatgcca aaaatatggt attaagagag agtcatatcc aaaaacttac gatgcaaaaa
    18961 acagttttac gtcattttga atgcagtaat gtcatttttg aaaaatgtga tttttccaat
    19021 ctggagtgga ttggtggcag tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt
    19081 acgaattttg ccgaaagtta tcttcgagac tgtactttca ctgattgtat tgcttcgatg
    19141 gcttccttta gtagtacaaa cttgaaagct gtctccttcg atcattgtca attagaagac
    19201 agcgaatttt atgaggtcac ttggaaaaac ctttttttgt ctgacaatca gttgaccggt
    19261 tcgaactggt ttcgaacttc tctgaaggga ttagacttta cgactaattt cttcacagca
    19321 atcgctttat ctcaagattc tttggcggga ctaatcgtca accaagagca agcacttgtg
    19381 atcgcccaag cccttggttt gcagatcaaa gactagatca acgaatccgc cgccacagtg
    19441 caataagcat ccaaatagat aagaccaaac tgatgatcaa ggcgattccc catccgaaaa
    19501 cggaatgggt aaagggtaac tggagattca ttccgaagaa tcctgtcaca atagttggaa
    19561 cggtcaagat cagcgaccag accgtcaaaa atttcatcgt gtcattcaag ttgttattca
    19621 gcaaattatt ataagtgcca gagagctggt ctaaaatctg cgaagctaag ttcgtcattt
    19681 caactgcttg tcgtgcttcg atcagcgcat cgtccaactc ttctttttcc ttttctgaca
    19741 acttgcgata aatagccaat gccttgattt gttctaaaag tactacattt tgttttgttc
    19801 ctgtaactaa gtaaaccaaa ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg
    19861 ttgttttttc tcttaatttc agattaagcc gctgacgttc actattcact tcctcgatta
    19921 aaggaaaaaa ggaatcagaa atcaaaaaca atgtacgaaa caataaactg tatacactca
    19981 tgtctggcat tcgttccaaa agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg
    20041 tggtaaatgt aaacaaaccg tcttctttta aaataaaagc gataggacta gtttcataat
    20101 gattatcgac tttttgctgg tgcggaacat ttgatacaag cagtaacgct tcatcaaaag
    20161 aatcgtactc tacgcgcgcc cgttcatttt tatctagaga ataagtcagc atctcgtcac
    20221 taatgccata ttcttctttt aagtttttaa cagctagcac atcgttttgc tgtacattga
    20281 tccaaaaatt acggtcttga ttaaaatagt gtttttcccg catactctat cctcctgaca
    20341 tttgtttcca gtataaagaa aaaagtcgct caaataaagg tttacccttt atttgagcaa
    20401 ctgtaaatca aatggctact ttggtttggc gaatctctgc taatttttct tcgatcattt
    20461 tcaagacttc ttcttttgct tgaggatctt ccacaaaatt atagcggtct ccatcgattt
    20521 ggatttttgg actttctttg tattcttcat accattgatc gtaacgtctg ttcaattctt
    20581 tgtaataatc gtatagactt ggatcaaagg acaattgttc atacgaacga ccacgttttt
    20641 cgatacgctc caacatcgta tcaaaagata cacgaatatg aactaataaa tccggatgtt
    20701 ttttatgggc agcataggga agttcttcca tcatgttttc cagcagttca tcatacacac
    20761 gaacttctgt ttctgttgct cttcctagat ctgcatttag atggaaaagc agtgaatctt
    20821 cataaatcga acggtccaat acattattgt catctttcat tgcttgtttg atgcttgcaa
    20881 aacgtttatt caaaaaataa atttgcagca aaaaagcata ctgttcaggg tttgcataaa
    20941 ataagggcaa gacttcatta tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt
    21001 cagccatcat ttctgttaaa cttgattttc cggcaccaat tgtacctgct aaaacgatca
    21061 cgctcattta cctccagctc acttcatcac taaatattgt gtttaactcc aaccttttta
    21121 aatatacccc caatatctag tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa
    21181 agtcaaaaaa ttcttaagaa aaaagtgttt tttctttaga aaacgtattc ttagttttac
    21241 ttctgagatg gttttagtaa aataaaggag gaatgttttt tgagaccctt agtggacaaa
    21301 aaaattcgtg aagcagcgca actcgttttg ggcgctttat tttttgctta aaaatcactt
    21361 ttacgtgaga aggagttaga ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat
    21421 catattgatg ggcgagcacg cggttgttta tggagaacca gcgattgcct ttcctttcta
    21481 tgcaacaaaa gtcaccgcat tccttgaaga gctggatgca atggacgatc aactggtttc
    21541 ttcctactat tcaggaaatt tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt
    21601 attcattcac ttaaaaaaac agcatgacat ccaaaaaaac ttgcaactga ccattgaaag
    21661 cacgattcct gctgaacgtg gaatgggatc aagcgctgca gtcgccacag cagtcactcg
    21721 tgctttttat gattacttag catttccttt gtctcgtgaa atactattag aaaatgtcca
    21781 gctttcggaa aaaatcgccc atggtaatcc tagtggaatc gatgcagccg ctactagcag
    21841 cttgcagccg atttatttta caaaagggca tcctttcgac tacttttctt tgaacatcga
    21901 tgcttttttg attgtcgctg atacaggaat caaaggacaa acaagagaag ccgtcaaaga
    21961 tgtcgctcac ctctttgaaa cccagcctca tgaaactgga caaatgatcc aaaaattagg
    22021 atacttgacg aagcaagcaa aacaagcaat cattgaaaat tcaccagaaa cgttggcaca
    22081 gacaatggat gaatcacaat cacttctgga aaagctgaca atcagcaatg attttcttaa
    22141 tctattgatc caaacagcaa aagataccgg ggcattgggt gccaaattaa ctggcggtgg
    22201 acgcggtggc tgtatgattg cattagcaca aacaaaaaca aaagcccaag aaatcagtca
    22261 agcacttgaa gatgcgggtg ctgctgaaac ttggatacaa ggattaggag tacataccta
    22321 tgtttaaagg caaagcacgc gcatatacca acattgctct aatcaaatat tggggtaaga
    22381 aaaatgaaga actcatcctt ccaatgaaca acagcctttc gttaacattg gatgcatttt
    22441 atacagagac agaagtcatc ttttctgata gctatatggt agatgaattt tatctagatg
    22501 gcaccttgca agacgaaaaa gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta
    22561 aagaagccgg cctctcacta aaagcttcag taatcagtca gaatttcgtt cctaccgcag
    22621 ctggattagc ctcctctgcc agcgggctag ctgctttagc aggcgcttgc aatactgctc
    22681 ttaagcttgg attagatgat ctctctcttt caagatttgc tcgacgcggg tctggttcag
    22741 cttgccgaag tattttcggt ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt
    22801 cttacgctaa gccagtccct tccgattctt tcgaagacga tttagcaatg gttttcgttt
    22861 tgatcaacga ccagaaaaaa gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa
    22921 catccagttt ttatcaaggc tggttagatt ccgttgaagg ggatctatat caattgaaac
    22981 aagcaatcaa aacaaaagat ttccaacttc tcggagaaac gatggaaaga aacggactaa
    23041 aaatgcatgg aacgacttta gctgcccagc ctccattcac ttactggtct cctgacagct
    23101 taaaagcgat ggatgcggtg cgacaattga gaaagcaagg cattccatgc tactttacga
    23161 tggatgctgg ccctaatgtc aaagttttag tggaaaacag ccatttatct gaggtacaag
    23221 aaacatttac caaattattt agcaaagaac aagtaattac tgcacatgct ggtccaggaa
    23281 ttgcgattat tgaataaagg gagattttta tgattgaagt atctgcacca ggcaaactct
    23341 atattgccgg agaatatgca gttgttgaaa caggccatcc agcagttatc gctgcagtcg
    23401 atcaattcgt gacagtcact gtagaatctg cacgaaaagt cggaagtatc caatctgcac
    23461 aatatagtgg gatgcctgta cgttggacaa gacgcaacgg agaattagtt ttagatattc
    23521 gggaaaatcc ttttcattat atccttgctg ctattcgctt gactgaaaag tatgcacaag
    23581 aaaaaaacat tcttttatcc ttttatgatc tgaaagtaac aagtgagtta gacagttcaa
    23641 atggccggaa atatggattg ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct
    23701 tgaatgtttt ttacgcgttg aatttatctc agttggagat tttcaagatt gcagcactag
    23761 ccaatttagc agttcaagat aatggttcct gcggcgacat cgctgctagc tgttatggtg
    23821 gctggatcgc tttctcaacc ttcgaccatc cttggctcca agaacaagaa actcagcatt
    23881 ctatcagtga gttacttgcc ctggattggc caggtctatc cattgagcca ttgattgctc
    23941 ctgaagattt acgtttattg attggttgga cgggtagccc tgcctctact tctgatttgg
    24001 tcgatcaagt tcaccgttcg agagaagata aaatggtggc ttatcagctt ttcttaaaaa
    24061 atagtacaga atgtgtcaac gaaatgatca aagggtttaa agaaaataat gtaacgttga
    24121 tccaacagat gattcgaaaa aaccgacaat tactgcatga tttatctgca atcactgggg
    24181 tcgtcatcga aacgcctgct ttgaacaaat tgtgtaattt agctgaacag tatgaaggag
    24241 ccgcaaaatc ttctggtgca ggtgggggcg attgcggaat cgtaattgtt gaccagaaat
    24301 ctggcattct tcctttaatg agtgcatggg aaaaagcaga aatcactcca ctgccgttac
    24361 atgtctatag cgatcaaaga aaggaaaacc gatgaatcga aaagacgaac atgtttcact
    24421 agctaaagcc tttcatgata aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa
    24481 ccctttgccg caaatagcag tttctgatgt cgatctaagt acacaggcag ttgggtttac
    24541 actaagcagc ccattttata tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat
    24601 caaccaagat cttgcaatcg tcgcaagaga agcagatttg atgattgcca ctggttcagt
    24661 cagcgctgcc ttgaaagtcc cttctttagc cgatacttat acgattatgc gacaagaata
    24721 tcctcacggg aaaatcattg ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga
    24781 agctatccga ttgtttcacg cagatgcctt acagattcat ttgaatgcac ctcaagagtt
    24841 ggttatgcct gagggagacc gtgattttac caattggaaa gtacttattc aagaaactca
    24901 aacagccatt gacgtccccc tcatcgtaaa agaagtcggt tttggcatga caagagaaac
    24961 gctcaatgat ctagctgctt taggagttca tacagttgat atcagcggtc gaagcggaac
    25021 aagcttcacc cagatcgaaa acgcccgtcg ctccaagcgg gaactgagtt atttagctga
    25081 ctgggggcaa tcgacagtct cttcattgct tgaagcaaat gaagcagaca cttctatgga
    25141 aatcctagcc tccggaggta tccgcaatgc ctatgacata tttaaagccc tttgtctcgg
    25201 cgcaaatgcc gtgggtactt caggaaccgt actgacgcat ctgatgaatc acggcgtaga
    25261 agaaactatt atactgatga agcaatggca agaagagctt cgtctgcttt atactatggt
    25321 cggcgcaacg aataccgcgg ccctacacca acaatctctg atcttttccg gaccagtgaa
    25381 ggattggtgc gaagcaagag ggatcgactt agtgaagtat ggaagacgca cagaaaagag
    25441 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtaca aacggcggaa
    25501 gtaaaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg
    25561 tgaattcctt cttatttctt ggagttaaat gcttctgtcc cagcctcttt tttcgaataa
    25621 atgatgttta atcagctgtc cttcggtatt cgttcaatcc gctgatttca caacctctcc
    25681 actaaacagt gtactaaagc tcttttaagg ttgttttccg atataggcaa gtatgcctcc
    25741 gtccacatac aagacatggc cgttaacgaa atcagatgcg ctagaagcca agaagacaga
    25801 tggtccagcc aaatcgattg gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa
    25861 ttgatcgaat ggatgacgtt caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg
    25921 tgtagcaata taacctggtc caattccatt acattggata ttgtattgac cgtattcaga
    25981 agcgatgttt ttcgtcagca tttttaatcc gccttttgcc gcagcataag cactgaccgt
    26041 ttcgcgtcct aattcactca tcatggagca gatattgatg attttaccgc cgcctttttc
    26101 gatcatatcc ggaataactg cttttgaaac gatgaatggg gcatttaaat ccacgtcgat
    26161 tacttggcgg aagtctgccg catccatttc gatcatcggt gtccgtttga tgatccctgc
    26221 attgttgact agaatatcaa ctgaacctac atcttctttg atctgttgaa ccatcgcttg
    26281 aaccgcttct tcatcggtca catcgcagac ataacctttt gcatcgatgc ctgcttcttt
    26341 ataatgcgct ataccttcat caactgattc ttgattcaag ttattaaata cgatttttgc
    26401 tcctgctgta gctaaagagc gtgcaatctc aaatccaata ccatagacag caccggtgac
    26461 taatgctact tttccatcta aacgaaacat atccatacta aattccatca ttttctctcc
    26521 cttatgctta ttttaattga tccatttgca ccatatccat gtctgtatag gtgatatttt
    26581 ctccgcacat tgcccagatg aatgagtaat tgcttgtacc tacacctgaa tggatcgacc
    26641 agcttggcga aataatggct tgttcattgt ctacaactaa atgttttgtt tcatctggtt
    26701 tgcccatcat gtggaacaca cgtgtatctt cattggcata atcaaagtag acataagctt
    26761 ccatacgacg ttcatgtgta tgacaaggca tcgtattcca agcgcttcct ggttctaaaa
    26821 tcgtataacc catttgtaat tgacagcttt cgcatacatt tggatggatg tattggtaga
    26881 ttttacgttc attcagtgtc aggccttcac cagtctccat tggttttact tggtcgatac
    26941 tgatttttac atttggatat ttatgatgag ctggaacaga actaatatag aatttagctg
    27001 ggttttcagc atcttgagaa gaaaatacaa catgtcgtgt ttcttttccg atatagtatc
    27061 cgtcttgttt tttcatttct tctttttgcc catcgatctc gatgaatcct ggtccaccga
    27121 tattgatcac acctagttca cgacgctcta agaagtaatc aacacctagt tctttgtcta
    27181 atgtgatttc taattctttt tcagtaggtg tcactccacc aaagatcatt cgatcgttgt
    27241 gcgtataagt caaactgatt ttccctggtt caaatacctt ttctactaaa aattcttttc
    27301 tcaattgttc cgttgaataa tgacggatat cttctggact gtgtgtataa cgtgtttcca
    27361 tttcttgcat tttcttctac tccttctatc gaatgatttt tccagagccg catgctaaaa
    27421 attgtgcgac ttcttcttga ctaaattggt tgcagtcccc atggatcgta tgcttcaagg
    27481 cagaagctgc cgtagcaaaa tcaatgattt cttgcggaga catattggat aaaatcccat
    27541 ggagcacgcc acctgcaaaa gcatctccgc cacctactcg atccacgatt ggggtgattc
    27601 tatggacttg ggattccaca tattcatggt tcatccacaa tgtccccttc aattcatttt
    27661 cacttgctga atgaacggta cgcttggtcg aataaaacac ctgaatattc gggaactttt
    27721 cctgcatttt ttgataataa taaattagag gttgttcttc tgcttctggc gctttctcga
    27781 tacctaacaa ataaagagca tccatttttc cagctgagca gatatctact aaaggtaaaa
    27841 gctgatggat tacttcgctg gcttcttgct gtgtccataa ttttccacga tagttgatat
    27901 cgaagctgat caggcagcca gctttttttg cagcttctat cacctgtttc gtcatttttt
    27961 gccatttttt agacaaagct ggggtgattc ctgaaacatg aaatatatct attccttgga
    28021 ataactcctc tagcgaccat tccaaatgat ccatttcagc aaaacttgat ccggcacgat
    28081 catagatcac tgaggcggcg cgttcgccac taccagtttc catataataa gtacctagtc
    28141 ttgatccacc agataatacc tgtttcgtat ctactccata acattgcaag tgctttttga
    28201 ctgcttttcc caatgcgtta tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat
    28261 tagctaaaga aattgctacg tttgcttccc ctccgccata atgggcagcg aatcgttcac
    28321 ttgaatcgat acgggttccc tgctctgtgg acaatcgcaa catgatttct ccgagtgtca
    28381 ctacttttcc cataagcagc tacctcttga tttctttcat ttttgctgca tactgttgcg
    28441 cgacttccgt tactttgtca aaatctcctg tagctgcggg tgctaaaagg ttcccaccta
    28501 ctccgactgt tacgacacca gcatcgaacc actctttcat attgtccaga ctcacaccgc
    28561 cagttggcat gatattgact tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc
    28621 cataagcgct tccaggaaat agtttgacga tatctacccc gcttttcagc gctgttttga
    28681 tctcggtaat agtcatacat cctggtaagt aaggaatttg atacagattg cacatctctg
    28741 ccgtttcttg atcaaaggaa gggctaacaa tatactctgc gccagctaag atagccaagc
    28801 gagctgtgac agcgtctaat actgtgcctg cgccaatcac tatttctggt tggtctttgt
    28861 agaatgacaa aagttctttg attacttgat ctgcttgagg gactgtaaaa gtcaactcaa
    28921 taccagttag accacctttg atgattgcgt ggcaagcatt caaagcttct tctttgcttt
    28981 ttccgcgtac caccgcaatc actccggcgt ttttcaatcg agataaaata tcaatttttt
    29041 tcatcttctc cacctatttc ctatttagaa attttatttt ataacttgga aatctatacc
    29101 catattatac ggcagaaaag aaaaattgca aacgcttttt tcattatttt tgaattcttt
    29161 gctcgatttc gctttttgtt tgcttgaggg cactgattat ttgttctttt gtctcatctg
    29221 ttaaacgata tttcggcata cttacactaa atgcaccaat gatttgttcg tttttcacga
    29281 ttgaggctcc tatacaaaaa atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg
    29341 tttcttttac cgttttgatt tcctttttca accgtaatgc attggtgatc gtatattctg
    29401 tgtaagggat caacggatgc ttttcaagat aatcagtgta ctgctcttca ctgaattctg
    29461 ctaatacggc tttgcccatc gcagaattgt ataatggtcg ggtgattccg atttttgaag
    29521 acatgcggat cgtttgatgt tttggatcta gtttgttcac gtacaagatc tcattgttgt
    29581 ccaatacccc taagtggata gtctcatcga tcttttgctg cagctgttct aaaaacggta
    29641 aagtcaactc taccaaatcg atttgttcaa tgtttttatt cgcgtagcga ataagctttg
    29701 cacctaaacg atagtttttc tctgaatttt tatttacgta gccgatcatc aacaaagtat
    29761 ctagtatttt caagactgta gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag
    29821 agacatcttg gttttccgct aaatagtcta gtattttaga ggctttgatc aatacagttc
    29881 cataaggctt tttattttcc atataatatt ctcctatttc tttataagaa aaattatagc
    29941 ggaaatacca tcaatttaaa agagatatcc agatataaac taccatttct ctttacaggc
    30001 aatttaatgt tgaagatccg cttcgttttt acatcaaact ttgatataaa gcaatgattt
    30061 cttctactgt cgtttctctt ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg
    30121 caattgctgg aatatctgct tcttttacac ctaattcaga aatggtttct gggattccaa
    30181 ccgctttgct caaccgatcg atctcaccac aagccgcttc tcttacatct tctaaagaca
    30241 tttcagctgc tcctttaatc cctagaacaa gagcgatctc acggtacttt tctccagtat
    30301 attctttgtt atatttcatg acagttggca aaagtgctgc acacgctacg ccatgaggaa
    30361 tgttgtacca tgcacttaac gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg
    30421 agaagcccat tcccgcaata tactgtccta atgccatttt ttctcgccca ttttgatcgc
    30481 catctacaga agcttccaaa gaatggccga tgatttcgat tgcttttaaa tgcagcatat
    30541 cagtcatttc ccaagcgcct tttgtgataa acccttcaat agcatgcgtc atagcatcca
    30601 tacctgttga tgcagccaat tttttcggca ttcccatcat catatcgctg tcgatgaacg
    30661 cgacgattgg gatatcatga ggatcaacac aaacaaattt tcggtgattt acttcatctg
    30721 tgatgacgta gttgatcgtt acttctgctg ctgttccaga agtcgttggt atagctaata
    30781 taggtaaaca tggatttttc gtatcagcaa caccttctaa actaatgacg tctgaaaact
    30841 ctggattcgt caaaataatc ccaatggctt ttgccgtatc gattggcgaa cctccaccga
    30901 ttgcgatcaa acagtctgcc tctgcctctt taacaaaagc cactccatct tttacatttt
    30961 ggatcgttgg attaggtacg atcccgtcgt ataaagcata agcgattgct gctttatcta
    31021 gaagttctgt tacttttgtt gctataccgt gctcgatcaa tcctttgtca gtaatgacag
    31081 ctgctttgcc gaatcctcgc tgctgaaatt cagggacgat cgactcaatc gctccttttc
    31141 caaaatacga tgtttcattt aaaatcattc gattactcat tttctattcc tcctaatttg
    31201 tattattttt cacaataaaa acgaaagaac cccaagcaac cggttgccca gttgcttgtt
    31261 gctacagttc aaagactggc atcaattccc aactgactgg agaggcatct ggatttgtct
    31321 ccatgatatc ttccatgtac ttccaccatt tttgattgat agatgtattt gccatttttt
    31381 cccataactt ttcatcttcg atcactaagt aaccaaataa caaacttgta ctcggatcaa
    31441 gatagatcga atagtcgatg accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa
    31501 gttcatcatg tctttttttg tattcttctt gtttttctgg gtaaactttc atgcaaaaag
    31561 cttttttcac catgtcagac acctgctttc gtatgaactt cctccaaata acctgctttt
    31621 ggagtgacac caaacgcatc tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag
    31681 attggtcctt gcgctttgac gtacgtatag acttctgctg ccttttctgc tgtctcgatc
    31741 aaaccaaaga cttcatccat gtcttttcct gacccgtaga ttccatgttg tggccataaa
    31801 accaatcgag tttctttcat tttttcagct gttgcttccc caatctcatt tgttcccgga
    31861 accatccacg gaatgatgga aattccttct ggaaaaacaa ctaagcattc cgtacacatt
    31921 tgccatagcg tacgactaaa ggaacgttcg tctaattcat gcgtaaaact catcgctaat
    31981 agatgactag catggcaatg cataacgatc cgattatctg ggtctacttc taaacgggcg
    32041 atatgactca tgaaatgact tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc
    32101 cacaaaagtt ccagtgtttt gccatcttca gcaactcgaa cgattccaag attctcggca
    32161 ggttcgctcg ctacattttt aaaatatctt cctgaaccag ttacaataaa atagcatcct
    32221 gctaaacggc tggcatcaaa aatcatcgag atgcggcgga tcactttttc aggatccaag
    32281 aacggtgtga tctcttcttc ttttagtaaa taagaaatat tcccgccatt tctttcatcc
    32341 catcccaagc gataaagatt cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt
    32401 aaaatatcta tttttttcaa tatagccaca ctcctatttt cttaattcca acacgtcttt
    32461 ttgatattcc tggacttctt ccagccaatc caaaccaact ggaacttgat ttttgtaaca
    32521 gaaataattc catacatcgg catacgggaa atctttcaat tcttccgtat aagccattct
    32581 tgaagtaaag tcaaaagcca gctccatttc tttcaatcgc tcaactggtt ccagcatagc
    32641 tttcagcagt gctttttgtg tatttctcgt accgatgacc caagcagcga tacggttgat
    32701 cgttgcatca aagaaatcca gaccgatatt tgttttttcc aacaattcgt tacggactaa
    32761 ttctttagcg atttcctgca attcatcatc catcaaaacc acatggtctg aatcccaacg
    32821 cactggacgg gaaacatgca acatgattcc tttgctgaat aaagctaatg aagataattt
    32881 attggaaatc acttctgttg gatgaaaatg tccagcatct agacaaacaa gtttgtcacg
    32941 agtcaacccg taacccatat aaaattcatg ggaaccgact gtataagctt cagcgcctaa
    33001 accaaatagt ttgctttcta ctgcatcttg tacataccgt tcatctaact cttctgtaaa
    33061 aatttcatcg agtgattcca ttaaacgttc ccgtggggta aaacgatcaa tcggattatc
    33121 tttcatccca tcaggtaccc aaaagttatt gatacttgtt tgcccagttt ctttcccaaa
    33181 gtactccgcg attttgcggc ttcttttccc gtgttcgatc cagtattcac gaacttcttt
    33241 atctggatga gccaatgtaa agccgtcttt catcattgga tgcgaaaaga aagtcggatt
    33301 gaagtcaaga ccgatttctt ctttttttgc ccactctacc catttttcaa aatgtttcgg
    33361 ctcaatttca tttagatcga cagattcttc tgtatccaaa tagatagcat gaaggttcaa
    33421 tttgtgtttg cccggtatca acgaataagc cttctccaga tcttgtctaa gctcttcagg
    33481 tgttcgtgcg gctcctggaa aattccctgt cgccatgatt ccaccggtca attcgccatc
    33541 cggtgtcaag aaacctttga catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc
    33601 ctgtatcttt tctagtacag catctgtatc tacaccgata gttgcatatt tttcttttgc
    33661 ttgttcgtaa tttctttcaa tcgtgttcat acgattactc ctttgatttt attcacgtac
    33721 cattccatcg tgttttgctt attttcatct gggtaaaaag tttcacatgg aaacgactgt
    33781 ttgattacat ttcttgcttg ttctatattc gaaaactgac ctgtactgag catttgcatg
    33841 gcaacattcc cgattgctgt ggcttcacta ggtcctgctt ctacaatgat tccggcataa
    33901 tctgctgtca attgattcag tagacgatta ttcgatccgc cccctacgat gtgcagcttt
    33961 tccaatttcg ttccagttaa gtgttctact ttgatcagct cttgtgcgta acaaagagca
    34021 agattatcat acacacaacg agcgatagct cccgatgaga cagggactgg ctgctctgtt
    34081 tcaaaacagt attgctgaat ctcttctatc atatttgggg gattcaaaaa tcgagggtca
    34141 ttgatttcca tcacttgctg cagcggtttt tcttttgcag caagctctgc caattccgca
    34201 taactgtact cgtactcttg cattcgggca acttcctgga tcaaccacat ccccatgatg
    34261 tttttcaaaa aacgcaccgt gttatgggca ccccattcat tcgtataatt ttcagaaaaa
    34321 gcattgcgag aaacattggc gatctttgtt tcaatcccta aaagcgacca tgtcccactg
    34381 ctgatataac cccaacgttc tccttgacct ggcgttccta atacagctga cgccgtatca
    34441 tgacttgcta ctgtgataaa ggtcacatct ggcaaatcat attttgaaaa ttgttctttt
    34501 tgcagatctc ctagtatcgt tcccggctct gtcaatggtg gaaataattc cttcgagaca
    34561 ccgatttttt caagaagttc tatttcccat tcttttgtcc ctgcattcag cagctgcata
    34621 gtagatgcat tagttttctc cgtcaccatg ttaccagtaa agacataccc taaataatca
    34681 ggtatcagta acaatttatg tgcttttgcc aaattttctt tttcttcagc aaacaattgg
    34741 aacaaggtat tgaaaggctg gatctgaatc cctgtctgct catacaactt atctagagaa
    34801 tagatttcag aaaactgttc tactgctcca ttggtacgct tgtcccgata agcaatcgga
    34861 tcatttatcc gatccccttg ttcatcaatc agacagtaat cgactgccca tgtatcgatc
    34921 cccgcaaaaa ggcgatcaat ccctgtctgc tttaattttt caaatccaat caaaatctct
    34981 ttgataagat aatcgatctt ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg
    35041 aaccggtgta cttcttccaa gatcaaagaa ccttttgcat ccctcgtact tttcattagt
    35101 cgtccgcttg atgcaccgat atcgatggca acaaatgttg gattcatttc tccacctctt
    35161 ttactttatt gttgattagc actttattaa taaagcgtta tcatttattg acaatgctat
    35221 cataatacag aatcttatat tttgcttcac actattttgg tatataattg cattatcttg
    35281 acaagttggg agtgtttaca tggattactg ggaaaaagcc aaccactcat ggtcggaaga
    35341 ctcaaaaaga gtgatcttga cgcctacaca aagaagcagg aatttatttt tttatatcca
    35401 agaaatcggt tacttcaaag caccaatccc ttactatact gaacgagcac atttaccttc
    35461 ttacttgata aagtttactt tgtctggcaa aggttcgctt acatataatg ataatcagca
    35521 acagctaaaa gctggagact tattttttat tgactgccaa gaatatcagc gttatcaaac
    35581 gatcagcgaa gaaccatggg aaatggattg gatccatttg aatggtcaga acgtccaatc
    35641 attttatcag gagttcacaa aagacggcca taacaccttc cattcaacag gagatccatc
    35701 agaaaacaag atccaccatt tgatcacaga attgatccac atgcaacaag gcacgaatgc
    35761 ccgtaccgat ttccagtcct ctgtagctat ccacgagttg ctgaatgaac tgattttgca
    35821 aaagtatcag ctagatttcg aagaagagga tattcccgcc tacgtttaca aactgaagca
    35881 atacttagac gaacatttca aagaaacgat tacacttgat caattagaac aacaattctt
    35941 attgaataaa tatcagctga gcaaagattt ttccaaattt atcggttctc caccaatcga
    36001 ttatttgatc agcaaaaaac tttcttacgc taaagacctt cttcggtata cagaatttac
    36061 ggttcgaatg atttccgaag aaatagggat tgagaatttt gcttacttta gccgcctgtt
    36121 caaagcaaaa acaggactat cgccgagaga ctttcgtaaa ttcagctgat tcatataata
    36181 tgtacgtcat tttttcaaaa tattttgaaa tatatagata aaaataaaat aaccgcagtt
    36241 tctgcttcta tgtaaacaac agcagctact gcggttattt tttagtgatc attctcattg
    36301 ttcatcttca aaatgttcat aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc
    36361 tttaggcgtt atgccgaact ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa
    36421 accactttta ctatatacag cactagagct gcggtattct cgcaacaagt gcttggcata
    36481 gatcagcttt ttcttcatta catactgatg aaacgtaaat ccagtgtact ttttaaattc
    36541 tcgtgtgata tggtatttgc tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg
    36601 atgagataaa ttttttgaga tatattgtaa catttgttcc acacgctcat taggaactac
    36661 agcattctgt tcttgcgcac tctcttcttt ttgtacaact tgattgagaa aaatcaagta
    36721 ttctaccaga gcttgttcat attttatgtc ttcaccgtaa atttttttat caggaggatg
    36781 gtcgataaat gataattttt gcttcaatac ttcctgttct atttgcaata cacgactcct
    36841 cttctctccc attggttgaa aacaagccga tagattagac catggcgtgg aacgactttc
    36901 tagaaaagaa ggggtgacaa agatatagac acgctcgaat acatctgaag attggcggac
    36961 gatacgatgc aaatcattgg aatggatgag cagcacactt cctgctttga tcgtaaattg
    37021 tcgtccgttc aaataaaaag tcgcttctcc ctttaatgta gcatggatct catagaaatc
    37081 atgatgatga tagatcgttt tattttggct gacgacatct ttgacatgat agatttcata
    37141 ctgatcagaa accatcgtat tgacttgttc accgctgatt ttcttttgga gcatgtgccg
    37201 cacctccttt tgttcactta tgcacaattt tattatacca cgttttttgt aatcaaaaag
    37261 agctgctttt atatactctt ataaaaaaga agagacgtgg catcttctag ccccgccccc
    37321 tccattctaa atattagtta accaacgtct tctttacatt attcaagaag acttcttcca
    37381 taacgaatcc catcatcttt tgtttcttca agaaccacat acaggtacgg acgattcttc
    37441 atgatgattt ctttgatttt tgtgtactcg atcacaccgt ctcccaaatt taccggcacg
    37501 attttttcct gttcgacacg aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa
    37561 cgttcaaaag cttcttctac caattggact tgttcttgat aatcactact tgtgatcaaa
    37621 ttggtcggat ctaaaatgat tcctagatag tccgaatcta ctgcttggat cagctgctct
    37681 acccttgcta aagaatacac cggatgattc aaacctggtt ctatccccac catcatttgg
    37741 tatttttctc cagcttttac caagcgacgg ataacggaca cagcttcagc aaatgcttca
    37801 tctgtgaaat tttcttccgt atattggatt tccggcaaaa cacatccagt ttccgaagca
    37861 accatcgacg caccaaagga agaagcatag cgaagatact gctcgaactt tgtcagcaac
    37921 tcttgtcgta tcgttaaatc tggatgaatc atattgatat agcaacttaa aattcctacg
    37981 gtcacttctt gcttttctaa aactcgtttg acataatttc ccatcccaga attgatctca
    38041 tttgcgccag aaggtatttc aggaaacgaa agacctaagg cgagctgcag tgtatgaatc
    38101 ccttgttctt ttgttttttc tgcaagatct tctatcgttt ggacttcgga aagatcatgt
    38161 cctctaattg taatgttcaa cataactatt ctcctaactc agcatattgg gtagccacat
    38221 gacgatttgc gggaagtaag taatcaaaaa caagacgact aagataaccc cataaaaagg
    38281 aagaagcggt ttcagcattg cctctgcttt tacgcctcct acacttgcac cgacatatag
    38341 tcctgtacct actggcggcg tgatggagcc tacacacaga ttcatgattg aaaacaaccc
    38401 gtaatgtaca ggatcaaccc ctacacttgt gacgattggt aaaaatattg gtgtaaaaat
    38461 caagatagca ggcgccacat ccataaacat acccaccagt aataacacaa gattgactaa
    38521 taacagaata ataaatttat tatccgttac gcccataatc aacgaactaa ttgcctgagg
    38581 aataccggta aacgccatgg cgaaagacat catggaagaa gtagccaata agaacatgat
    38641 cgtccctgac attgctactg attgaaacaa catattaggt atatctttta gttttaccgt
    38701 tttatagtaa aacatagcaa tcaacaggga gtaaatgaca gctatcgctg aagcttctac
    38761 tgctgtaaat atccccgtca agataccacc gataatgatg acaatcaata gaaggctagg
    38821 aatcgcctct tttattattt ttccagcatg agccatctgt cctttttcga caacagggta
    38881 tttattcttt ttagcaacga tgaatgctac tagcatgatt cctagcgccc acaaagcgcc
    38941 tacgagataa cctcccataa acaaagcaga gatcgaagca ccaccagcaa caagtgaata
    39001 catgataaaa gcagtacttg gaggaatgac catacctgtc ggagctgacg cgatattgac
    39061 cgctgtcgca aacttccggt catatccttc ttccacttgc tgagggatca acactccgcc
    39121 aatcgcggta gaagcggcaa tggccgagct agagatggaa ccaaataaag cattccctaa
    39181 cacattcgta tgagccaaag cccccggcac gcgcccgacg aacaacatcg caaaatccac
    39241 tagtttctta gctatccctc cattattcat gataatgccc gagagaacga aaaacggaat
    39301 agcgatcagc gaaaaactat tgatacttcc caccattttt tgagctgaac taaaagcaga
    39361 aacatcaaac ggtataacta acaataaagt catcatcgat gagaatgcaa tacttatagc
    39421 aatcggcatc cctaatacaa gcaacacaac aaatacaaaa aataacacta atccggcttc
    39481 tattgccatt ttattcctcc atttttatat tttttcttga attgctggct tgttgcttaa
    39541 tgtatcgatt agattgatca ggctatagat aatgatgagt acccccgata caggtactga
    39601 cagataaaca tatcccatag gaatacttaa agaaggcgag atctgcccca ttgtcaatgt
    39661 gacagcattc catccgccat acaccatgat catcgcagca aataccagaa ataaactttg
    39721 aacaacaact tctaaaattt tacttttttt gccagttaat ttttcactta aaaccgttac
    39781 ggctaaatgt gctttttgtc ccacaacata cgcggaagac aacatggcaa accagacaag
    39841 accgaatcga acaagctctt ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa
    39901 tacttgccat aaaacaacag cgaccatccc aacaagcaaa acagctccca ggatctctaa
    39961 taccttgttt aaaatcattt tcatcacagc tacgctcctt gctcttggat cttttgataa
    40021 aggtctttgt aggtaggatc gtttttgaat atatcatgaa gtggggcaac tgcctctcga
    40081 aacggtgtat tatctacatc attgaattgg actccatgtt cttcttgtgc taaagctttt
    40141 tcttcttcaa ttgcttttgc aaaagcttca atttcaaaaa ccgttgattc tttcgctgct
    40201 tcttcaatgg ctttttcctg ctcatcagtt agctgttctt tgatggcatc attcatgatg
    40261 atgatatctg gcacacgtgt atgctcatca taggaatagt atttagccac accaccatgt
    40321 ccagctgtat acaagacaaa ttcattgttt tctgatccgt tgatcaaatt ggattgaaga
    40381 gacgtgtaca cttcatcact tcccataggt acgggcgaac cgcctaaaag ctctaccatt
    40441 ttgatagcgg tttcgctttg catcacacgg atcttctttc ccttcaaatc ttccggcgta
    40501 tggatcggac cgtctaccat ataaaagctg cgctgaccag aatcataata ggtcaatccg
    40561 gtaaatccta aatttttggt cgattggtag attggctcca ttatttcttc attatccatc
    40621 accttaaaga aatgttcttc atcattgaat agataaggaa ctgaaaaaat cgaataatct
    40681 ttagagaaac tttccaacgc tgaggcactt acttttgtga aatctactgc tcctgactgt
    40741 gtcaattcga tcagttcacg ttctccaccc agttgtccat ctgggaaata ctggatctgt
    40801 acggaaccat ctgttttttc ttttactaaa cgcccaaatt cattcattgc atcaattact
    40861 ggctggctgt tgcttgcata agcaaagcgc aaggtaacta tctcatttac atctgcatct
    40921 gcgacagttg ccacactaga attatttccg cagccgctta gacctagcag acctaggcta
    40981 agcgaacttg ctgcaaacag cagtgatgtt tttttcatat gggtttcctt tcttttaaca
    41041 ataagagacg agcaattctg tcaaacaaag caccgttaac gactgtccat aaggcattgc
    41101 tgtgattccg atattccgat aaaaatctaa actgtccccc attcctgtcc cgattgaaac
    41161 gttttgaact tctccttttt catcgatttg ttcaagcaat ccttggatag cacgataagc
    41221 gacttcttta tattcttgtg gtaaataacg tttatggacc gctttcaaaa taccgtaagc
    41281 aaatcctgca gttgcagaag attctacgta agaagaagga tcatccaaca acgtatgcca
    41341 taaaccgctt tcagactgat aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat
    41401 caataatttg cgcaatgcat cttctttcga taattctaag atttcaatga tctctggaat
    41461 agcaatcgtc aaccagcagt ttccacgagc ccaaaatgct tcggcatagt tatgatttcc
    41521 ttcaaacgtc cagccgtgat accacaatcc tgttcgttta tccatcaagt atttgatatg
    41581 gatcataaac tggtaacgag cttcttctaa atattctggg cgattcaaca gtttcccgat
    41641 ttttgccaaa ggaagcactg tcatcatcaa tgtatcgtcc cacaactgat tcttattttc
    41701 cggtccatat gtggcatgct gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac
    41761 ccattctgcc cattgttcta aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa
    41821 gtaagccata gtcaatagcg gtgccattgt attgacgttt ttgggcggag cgccttcttc
    41881 catacgatcc tcaaaccatt cattgaccac ttgataagcg cgaggatttt ttgtcatctg
    41941 gtaattttta taaataccat atagaccgac accttgtggc caattccaca ctgtccagct
    42001 tttgtcatct actttcagtc catcaaagtc caacaaaaat tcaccattat cgtctttgat
    42061 cgttgtcagg ttttcaacga gcaggtcaat tttttctttc actaattctt taggtacaaa
    42121 aatctcttct ttaataatca tctttacgcc acctttttaa acgctttcat tttatatcct
    42181 taatatagca gtaataccgt ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa
    42241 ttgacctaaa tgttaaataa caaaagtaac tttcagttac ttggaaatta aaaaaagaac
    42301 ttggagaaac aacgttttcc aagttctttt tcatctattt tcttatcttt tttctacgct
    42361 ttcttttgct tatccgttgt aagcatcaat gatgatctgt ttcaattcag agatcaatgg
    42421 ttctttcgga ttagctgtcg tacattggtc ttcgtaagct aattcagcca tacggtctac
    42481 ggttgtatct agtgtttctt gtgtgactcc ttgagctttt agactcatgt tgatgccgac
    42541 agattttcct aattcataaa cagccgttgc taaagcttcg actaattcct ctgttgtttc
    42601 gcctttcaga cctaagaatt ttgcgatatc tgcgtaatct gtatctgcac ggaaatagtc
    42661 atatttaggg aacatcgcat gtttttgtgg atcttttgca ttatagcgga taatatgcgg
    42721 taaaagaatc gcatttgtcc gtccatgagg gatcccgtat tccccgccga ttttatgtgc
    42781 gactgagtga caaataccta ggaatgcgtt agcaaacgcc ataccagcca ttgtagaagc
    42841 gttgtgcatt ttttcacgtg attccatatc aggtgttttt actgattttt ctaaatagtc
    42901 aaagactaac ttgattgctt gtaggctcaa tccacgtgta taatctgaag ccatgactga
    42961 aacataggat tcgattgcat gtgttaacac atccatcccg gtatctgctg tgacagatgc
    43021 tggaactgac atgacgaatt gaggatcgac gattgcgaca tctggcgtca atgcataatc
    43081 tgctaacgga tatttcacat gtgtttcact gtcagtgatg accgcaaatg gcgttacttc
    43141 agaaccagtc cctgatgttg tcgggataca tacgaattgt gttttttctg gtttgtcgat
    43201 cttatatgtc cgtttacgga tatccaagaa tttttgtttt gcaccaaaga aagatgtgtc
    43261 tgggtgttca tagaacatcc acattccctt agctgcgtcc attgcagaac cgcctccaag
    43321 agcgattact gtatctggct tgaagtctgc catcagtttc gttcctgcgt atacggtatt
    43381 tgtcgatggg ttcggttcaa catctgagaa gacttcgatc ttcacatcat ttttacgtcg
    43441 tgccaatact tcacgaactg tatcgcagta accgaattgg accataccag ggtcacaaac
    43501 gatcatgaca cgttcaacgt tttccatttt ctctaagtac aatagagagt tcttttcaaa
    43561 gaagattttt ggcggtaatt tgaaccattg catgttattt ctccgtttcg ctacagtttt
    43621 gacattgatt aagtttacag cagagacgtt acgagaaaca gagttcttac cataagaacc
    43681 gcatcccaaa gtcaatgacg gaatcatttc gttatagata tcaccgatgc cgccttctgc
    43741 agatggtgta ttcactaaaa tacgacaagc tttcattcga agaccgaatt tgacatgcaa
    43801 ctcttcgttt tctgaatgga taactgctgt atgtcctaat cctccaagat tcaacatacc
    43861 ttcacacaaa tcaaacgcat ggtccgtgtt gttagctttg atcatggcaa gaactggaga
    43921 aagtttttct cttgatagcg gataatctgg gcctacgcct tccaattcag caatcagcat
    43981 tttcgttcct tttggtacac tgattcctgc caattttgcg atttccatcg cagaatgtcc
    44041 aacgattgat ggattgaccg catattttcc ttcgttcatc actgcatctt ctaatttttg
    44101 caattcatct ggttttacga tataaacttg gtgtgcttgg aattctgctt ttactgctgc
    44161 atagatttct ttatccacga tcacagcttg ttcagaagca caaatcattc cattatcaaa
    44221 tgttttcgaa acgatcaaat cgttcactgc acgtttaatt tttgctgttt tttcaatata
    44281 agctggtacg ttacctggac ctacacctaa agctggtttt cctgtagagt aagcagactt
    44341 gaccattcca gctccacctg tcgctaagac gatcgcgata cctggatggt tcattagcat
    44401 agaagtcgct tcgatcgaag gatgttcgat ccattggata caattttcag gagctccggc
    44461 agcgatagca gcatcacgta ccactcgtgc tgcttctgca gaagattttt gggcacttgg
    44521 atggaaagca aaaacgattg gatttcttgt tttcaacgcg atcattgatt tgaaaatcgt
    44581 tgtggaagtc ggattcgttg ttggagttac tccgcaaacc acaccgactg gttcagcaat
    44641 ttcaatcaac cctttttgga catcttcatt gatcacacca actgttttat catgtttgat
    44701 attatgccaa atatattcgg acgcatacat atttttgatt gctttatctt cgtaaacacc
    44761 gcggcctgtt tcctctacag ccatcttcgc taatggcata tgttgatcca aagctgccat
    44821 tgccatttca tgcacgatat ggtctacttt ttcttgatca aagctttcca tttctttcaa
    44881 tgctgcattt gctttttctg ccaaatcatc aatcattgct tgtacgtcaa ttgtcttcgt
    44941 tttttctttt tctattactt tagccatcat tatcctccta atgggtcatg gttatttgtt
    45001 aaataattca catgtatatc atacaatatt tatttttcta tgtaaacact tttttgtgat
    45061 ttttttcaca gttattttta aatataaaat tgctttattt acaggtgttt gtatagaaaa
    45121 atatataaag ttatttatta ttatttatat gacacacccg attattcaat attgagcaat
    45181 atatcccatg ctcttatctc tttctttttt gaataaaaaa tgaggtagga catctttctg
    45241 ccctacctcg tctttaaata agctttgata cttatttttc ttccttctct tctgtctctt
    45301 ccagcgtttc tgttgtttca gtttttactt caactgtctc agctggttct tctgttgttt
    45361 caacagtagt cgttgcatca tttgttacga cagtccctgg tttgaccgtt ttgattgctg
    45421 cacgatcgta ctcaaggaag atcccttcgc agtctaaaac aactgtccgt ttgtcattgt
    45481 cgatttctga aacaactcca tgtaatccgc cgatcgttac gactgaatca cctggtttca
    45541 tgctatctaa caagttttga cgttcttgct gttgtttctt ttgcgaacgt gacataaaaa
    45601 accacattgc taatagcgcg ataaacatca caagcattgg taatgttccc atttttttca
    45661 cctcactaat tctgtatcta caaaaaacac tttagcagaa aaatgctgat aacactagtt
    45721 ttttacttaa tttttaaaag attttccttt agaagctttt tgcgttttct ttattgaacc
    45781 cgtattcttc aaaaaaggct tctctaaatt caagcaaatt atcatccatg attgcttgac
    45841 gtacttgttt cattagattc aacaagaagt ataaattgtg gtaagaagtc aaacgaatcc
    45901 caaatgtttc atcacatttg atcaaatggc ggatataggc acgtgtatag tttttacatg
    45961 tataacaatc acatttctca tcaattggac ggaaatcatg ggcatatttc gcatttttca
    46021 ctactagacg gccttgagaa gtcatacatg tgccatttcg agcgattcga gtaggcaaga
    46081 cacagtcaaa catgtctact ccacggataa caccatcgat caatgaatca gcagcaccga
    46141 cacccatcaa gtaacgtggt ttgttttcgg ggatcaacgg tgtagtgaat tctaatacac
    46201 gattcatttc tgatttaggt tccccgacag ataacccgcc gattgagtaa ccagggaaat
    46261 ccatcccgac tagatctttc gcgctttgac gacgaagatc ttcaaaccct gctccttgga
    46321 tgattccaaa taatccttga cggtctggat tagcatgagc tttcaatcct cgttctgccc
    46381 atcgagacgt acgttctact gattttttca cgtagtcgta actttcatcg aatggtggac
    46441 attcgtcaaa actcatcatg atatcagagc ccaatttatt ttggatgttg attgcttttt
    46501 ctggtgataa aaacatacgc gaaccgttca agtgattttt gaaatgcacg ccttcttctt
    46561 cgatattccg catatcactt aatgaaaaaa cttgaaagcc gcctgaatct gttaaaatcg
    46621 gttgatccca gttcataaat ttatgcagtc cgccagcttc ttctactaag tcttcaccag
    46681 ggcgtagcca taaatgatat gtattactta aaataacgcc agcacccatt tctttcaact
    46741 cttctgggga cattgttttt actgtagcta atgttcctac tggcatgaac attggtgtgg
    46801 ggaaagttcc gtgtggcgtg atcaattccc ctaaacgcgc gccagtatgt ttttctttct
    46861 tgattaaacg atagcgaatg gcaggttgcg tcattcattt ccctactttc tatataaaat
    46921 atcttgacaa ctcccaccat cataatcgtt tcaagtgctt tttgcaagtt ccctcggctt
    46981 ccttctactt tatttaatat tctttttttt atcctttttt tatgaataac aaaaaaaaga
    47041 aaaacattat gacagaaata ttttttgata catggtatat tggtgttatt aaagaaaagg
    47101 agggaaaaat catgcaatct tctgcagaat taaaacgcca agcaaaagag agtctaaaag
    47161 gacgttgggg acaagctgtt ttactaaatc taatccctac attgattggt attgctacta
    47221 ttcttcttat tatgattcct attatcttgt ttgccaccac catatatgat ggatcagcga
    47281 gcgtctcaga agcgacaact tcaggaggcg gtagttcagg aggcggtggc ggaattattt
    47341 ccagcatcat tagcgctttg ttcatgagcg gtatttcttg gacttatctt gatattttac
    47401 gtggacaaaa agatcagatc gaaccattca aagatgcatt tcgtggattc aaaggtttgt
    47461 ttatcggcgg agtgatcagc ttagctgtgc tgatctcgat ctttacaaca ttatggggtt
    47521 tgctattagt tattcctgga atcattaaaa gctattctta ttctcaaagc tattttattt
    47581 actacgatat cgtccaagaa acaggacaaa cacctaaagt actggatacg atcactgcaa
    47641 gtcgaaaatt gatggatggt tataaaggac aacttttctg gttagatctt tcatttatcg
    47701 gctggcatat cttagcatta gcaactgctg gtattggtta tctatggctc agcccataca
    47761 tttctgcaac aaaagcagca ttttatgaac aattgccaaa agatatttaa aagagagtgt
    47821 gacttgtgac cgagggggtg gcaggagaac accgtttatc gagaggcagt aaaagaaaca
    47881 ttcagcccat tagcaaaaag gaggtgtgac aacattcatg tcacacctcc tttttgtata
    47941 gttccagtaa aaagaaaatc aacttagaaa cagttaatca ttcctcttct tctgcttccg
    48001 gtaatcgatg gaacttgatc agttcattca ccgcgattgg tacgatagat aaaatcccta
    48061 ccaagaacca gtgattcaaa ctgatgtgta ctaggccgaa tagttcttgc gtaaatggca
    48121 gcaaagcaac agccgttgta atagctaatg ccagtaacgc catttctacc aaagctttat
    48181 tggtcgctaa attgactttg aagatcgatt ggctgctgcg tacattgaac acatgcagaa
    48241 tcgatgaata agctaagatc aagaatgcaa cagtttgacc gacatgctga cttgcgtcta
    48301 cccatgggct gatagtatcg acataactac ctaaataata accaaacaag gttacgatgg
    48361 tgaagacccc tgcatttatg gcaatttttt gcaataaacc tcgagcaaag atcccttcat
    48421 ttttcggaat gggtggctga tccatgatgc cgtgttccgc tggctctctt cctaaagcaa
    48481 atcctggaag tccatcagca actacattga caaacagtag ttgcacagct gtaaatggtg
    48541 ctccccatcc taacagcatc gcaatcaaaa caataaagat ctccgaaata ttacagctta
    48601 gcaaaaagtt gatagcttta cgaatattct gataaaccga acgtccttga ctgacagcgt
    48661 ctacgatcgt cgcaaagttg tcatccgtca aaatcatatc tgcggcactt tgcgccacat
    48721 ctgttcctgt gatccccatg gcacacccaa cgttacttgc tttcaacgct ggtgcatcat
    48781 tcacaccatc cccagtcatc gctacgactg cacccgtacg ttgccaagat ttgacgatcc
    48841 ggatcttgtc ttctggcgtt acccgagcat agacagagag agctttgaca cgcgcatcta
    48901 attcttcgtc tgataattga tgcagttctg atcctgaaag tgcttctgac ggatcattca
    48961 agatacctaa ttctttcgct atggcactgg cagtcaccac atgatctcca gtgatcatga
    49021 ctgttttgat gccagccttt ttcgctcgct tgattgctcc tttactttct ggacgtggtg
    49081 gatcgatcat tccgatcaga ccaatcaaac gtaagtcttt ttccagtgct tcagaagaaa
    49141 tatcttgcgg ttcttcgtca taagtagcat aaccaacagc aatcactctc agtgcccgtt
    49201 taccaaagcg atcattaacg atcgccgctt gttctacatc gcctgaacta aagcgcggag
    49261 caagaacatc gaatgctcct ttggtgattg agatgtattt ttcgcccatt tggtgaactg
    49321 ttgtcatcat ttttcgttca gaatcaaaag gaagctcgtt tactcgtgga tatttttctt
    49381 ctaattctgc ctttgtatga taattctctt caacagccct tacgatggct acttctgttg
    49441 gattaccttt gataacaagg tcgtcatctt cttgctcgac cgtcacatct gtacatagtg
    49501 cagccatttt caatatttcc atcgcgctgt tcgtcattga atcttcgata ttcgttactt
    49561 catcttcttt gctccatacc cggcggacgc gcatttcatt ttgtgtcagc gttcctgttt
    49621 tgtctgagca gataacatta gcggttccta gcgtttcaac tgctggcaac tgtcggatga
    49681 tcgcatgttt cctcgccatc ttctgcacac cgtatgcaag tgtcaacgta acaataacag
    49741 tcaaggtttc cggtacagct gcaacagcca aggaaatcga agtcatgaac atctcaagga
    49801 gtggttctcc ttgcagttca ccaatcacaa atactaaagc cgcagccgct aaagcgatca
    49861 tactgattcg tttgcctagc tgattcaatc ggacctgtaa aggtgttttt tgcatagtat
    49921 tttcgttcaa aagatccgcg atcttaccca tttctgtagc catgccaatg gcagtgacga
    49981 tcgctttccc acgacctgca gcaaccgtac atcctttaaa taccatatta agttggtcac
    50041 ctaatgactc atcttcttca gcaataaatg ctgcatcttt ttcaactgct tcactttctc
    50101 cagtcaacgc cgattcttct acacgtaatt gtgatgcttc gatcaaacgg gcatcggcag
    50161 aaatagcatc accggactct aaaacaagaa tatcacccac taccagttgc tctgactcaa
    50221 cagtggtcac tttgccgtct cgtatgacag ttgccgtttg tttattcata tcttgcaagg
    50281 attccagtgc tttttctgcg tttccttctt ggacaatcgc caataccgag ttgatcacaa
    50341 cgatagcgat gatcagcagt ccttcaaata aatccccatg ttctgtagca aatgcggtat
    50401 aaaaggaaat caccgctgct acaagaagga tgatcgttgt aaaatcggac aagcttcgaa
    50461 agaatttttt gatcatcgat tctttcggcg cctcatcgaa tttattcaat ccctgctttt
    50521 ttcgccgttc atccacttct tgtgaagaca acccagtatc tggatctgct tctgttacct
    50581 tctttacttc ttctattgtc tgcttgtatt cttccatcct attcctcctt taaaatcttc
    50641 gtacaaaaaa aggtacttat tttactataa catccttttt ttatttactt caaataaagg
    50701 gcttatcgat tatttgttaa gaaagcgtaa aatttaaagt gcttatattt gcaaacgcta
    50761 ccaaaggatg ctattatata gatgtaagga aggaggatac catcaaatag acagaagaat
    50821 ttctcgatag aacagtgtaa gcaaacaaag ttgttagaag tacaggtttc acccagacat
    50881 gcgtgtagag tacaaaccta taaatagtga atgcgtatgg atgtttggat cacgaagtat
    50941 ccgaaacaag aagtaatgcg atacgtaaca attgaataac gtaaacgtct gaaaattgaa
    51001 taggaacaag agatgtttca aatgttgaag atgtaacgaa aacaaaattt ttgtttaaca
    51061 tgaagaaatt caacaccgtg ctttgcatgt atgatggaaa gttacaatca tttctgttta
    51121 tttgaatcct tccgaggtac aagaaagccg agcttatcga gagctcggct ttttctttgt
    51181 cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca taaccttatt
    51241 tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc gcatgctggt
    51301 aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact aaagtcgatt
    51361 ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag ccaggcgtaa
    51421 tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc gttccaatcg
    51481 tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca tttttacgta
    51541 cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta tgcatatggt
    51601 gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca
    51661 gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc gtaaaatgca
    51721 atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt tgatagcgat
    51781 ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc aatgattcaa
    51841 gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg tgttccaatt
    51901 cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc ccaactttgg
    51961 cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc gttagaagaa
    52021 ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca ggcagaactc
    52081 gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt tcatggaaat
    52141 gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa tgatcgcggt
    52201 cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa tcttctgttg
    52261 ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg tttttcgttc
    52321 tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata aaaaaactac
    52381 cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct tcattgaaaa
    52441 tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg
    52501 aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca tttgttttat
    52561 cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa tgactgatcc
    52621 aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc cagtggaatt
    52681 gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca tgacgatccg
    52741 tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac ggaataaaac
    52801 agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc gctacccaca
    52861 tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca
    52921 agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc gtgattcctt
    52981 gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca
    53041 tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt
    53101 gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga
    53161 gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag tcggcgaagt
    53221 tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag caatcaaact
    53281 gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg tattaaaagg
    53341 atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat
    53401 tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata ccttgcaacg
    53461 cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg
    53521 tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg gagaagatcc
    53581 cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg tctgacctat
    53641 gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg aacatcttta
    53701 tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg gtgtcttact
    53761 gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct tgcagacaat
    53821 cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta aattacctgc
    53881 gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt acattgggat
    53941 gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga ctgagttcca
    54001 atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg gtattcgttt
    54061 agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg ctggtggttt
    54121 aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt tggcaggtac
    54181 gatccctgta acaattttag cactattggc tgattggctt ctaggtctct tggaacaccg
    54241 gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact aaaaatgacc
    54301 atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct tgccagcaat
    54361 acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca gatcttagca
    54421 agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat catcaacaat
    54481 ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc aatttcagcg
    54541 gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga aaaagatccg
    54601 aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca aacgtggttc
    54661 ccgtcctata gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa
    54721 taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt agctggtgta
    54781 gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga aacttatggc
    54841 ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc agtcgaagca
    54901 ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag ttatgatcta
    54961 gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc agttgtagac
    55021 aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt aggtcatacc
    55081 atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct agtggaacca
    55141 tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa gtgaagcaat
    55201 tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat ggctcctata
    55261 tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc tttgcagcag
    55321 ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca aactgggtga
    55381 ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc attttaatca
    55441 ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat tccctcttac
    55501 cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc cttgatgtcg
    55561 gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta ccactctctg
    55621 tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg ataaccgcga
    55681 tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga accaatgcaa
    55741 cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg gcgatcatca
    55801 cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt acatcacgct
    55861 gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa ggctttttta
    55921 gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg ggcaaagagt
    55981 tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc aacgggttca
    56041 tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc caatccattt
    56101 tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc tgcttgttct
    56161 ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc cttttccatc
    56221 agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt
    56281 aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg ctaaaaacat
    56341 atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc ggtacacgat
    56401 tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt caccagaaga
    56461 gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt ggcgtaaaag
    56521 atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga aagcgccacg
    56581 actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt ggatacccgc
    56641 tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc aaaagaccat
    56701 gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat tgcttttttc
    56761 aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc ctcactttgg
    56821 cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat tttactgatt
    56881 ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc cattcctaaa
    56941 ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa tcatgcaata
    57001 aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga gactctttgt
    57061 taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag gcagaataaa
    57121 tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc
    57181 tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat
    57241 atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa ttcgtttgct
    57301 tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag ttactcatca
    57361 aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc
    57421 tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca
    57481 gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa tctcgatttc
    57541 agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag cagtgctagg
    57601 attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta tagcgtactt
    57661 gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt ttcctctttt
    57721 ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca cttcttactc
    57781 ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca tctttagcaa
    57841 aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag gttcctcgtt
    57901 gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag atttgtaacg
    57961 gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg attgcctctt
    58021 tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg ttgggtttgt
    58081 gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac ttgacgaggc
    58141 tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg aagaagctga
    58201 aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc aagacatcaa
    58261 aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc tgttcccatc
    58321 tttaatggct tatagagggc acctttagct gagaataaga caaaacagac cctctagctt
    58381 caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc ttcaaatggt
    58441 agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt aaccgcaaac
    58501 ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga ggaatcaaca
    58561 cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca taacggataa
    58621 ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca tctgggaaat
    58681 gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct agcgaaacat
    58741 gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc
    58801 gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt agttttgcat
    58861 cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc ttataaaaaa
    58921 tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac cgatagacaa
    58981 acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca atcgatttgc
    59041 ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga gataaagcaa
    59101 atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag ccatggaaaa
    59161 taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa tagcctagtc
    59221 gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa
    59281 gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc gctgtagcaa
    59341 attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt tgagacaaaa
    59401 cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg atccccacga
    59461 agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt tttgctcttg
    59521 gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa gaggaagaac
    59581 cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc gctcctggaa
    59641 gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca acaacgatca
    59701 gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat gcagattccc
    59761 tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa atagcgactt
    59821 gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct gagacagata
    59881 ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag ccagcattct
    59941 ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa gcatggatga
    60001 tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg gcaatgggta
    60061 gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt tgttccgtac
    60121 catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca aatcgggtta
    60181 atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac ctttttaagt
    60241 aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag tcgaaaattt
    60301 atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa
    60361 ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa tacagcaaaa
    60421 atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat agaacttgac
    60481 ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag atgtaaggct
    60541 gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa cctcttgata
    60601 aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa aacataagaa
    60661 gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta caacctcttg
    60721 gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc aaaatatgag
    60781 gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt cacaacctct
    60841 ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc attttttctt
    60901 cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa ccgatcatga
    60961 tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc cgtaacaagt
    61021 ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc cctgagactt
    61081 ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata atagcgatcc
    61141 ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg aacgctaagg
    61201 cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact cctagtaaga
    61261 aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca
    61321 tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt
    61381 attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat agtgcagtat
    61441 aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg gtttgtagct
    61501 caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat aatttgatga
    61561 cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct ttttggatac
    61621 cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc acagctgttt
    61681 cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca ttttttagtg
    61741 ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga atgatcggta
    61801 attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta tccatatttc
    61861 cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt tctttttgat
    61921 atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg agaagcgaat
    61981 caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga
    62041 cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata ccagcaatga
    62101 tgccattgat attattaaca acggcatcaa tttgatctag caattcatca atagccttcg
    62161 tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga ttgcttagca
    62221 agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt ccagcacttg
    62281 tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt tgagatattc
    62341 cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca gtcagcttac
    62401 catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc
    62461 caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca tctttcagct
    62521 gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct aaatttttca
    62581 catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt gcttcatcga
    62641 ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct gctagttgct
    62701 taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt tttgcttgat
    62761 ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc tcatctactt
    62821 tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt tcaggtagtt
    62881 gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg
    62941 ttgataaaat catatcttta actttattga tacttactag gttcgtatcg atgtcgtaac
    63001 ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat tcatttgtga
    63061 tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc gcattgattt
    63121 tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta aaactcagca
    63181 gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg gaacggactc
    63241 cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta tttttatgaa
    63301 gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct gctggcttgt
    63361 ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc cataatgctt
    63421 tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc agaagtgttg
    63481 caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc gtatttttga
    63541 ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac catgtatttc
    63601 atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat cacggcaatt
    63661 gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct ttactttatc
    63721 atgaaataaa agggaaagat aattttatgc caaagttttt atctattata cgtatacgca
    63781 aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt
    63841 ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag gaggatgtcc
    63901 ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat tcattgaaat
    63961 aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg cttctgcatc
    64021 tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt gccgcaaatc
    64081 tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga tctggatgcc
    64141 ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa
    64201 ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta atttttttac
    64261 cttttcctca tttttactgg atgcactggt taagattcgg attccctttt catggagaag
    64321 ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag tatctgtcgg
    64381 ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg tgaccattcc
    64441 ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg atggcaacac
    64501 gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac ctacatgaac
    64561 agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac ctggtacata
    64621 agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa catcataagg
    64681 attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt ctctgggatg
    64741 agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg caaatcttcg
    64801 catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt tgacaaaaaa
    64861 aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg
    64921 tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa aaataattgg
    64981 aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg
    65041 attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg
    65101 cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga
    65161 cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg tgaattagca
    65221 cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc tggctgtaat
    65281 gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac gttcgcttct
    65341 tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca gctggtcctt
    65401 ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca ggagtcagtg
    65461 cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca
    65521 aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc tccaacagga
    65581 taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga gtcggaagct
    65641 taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta aaatggttac
    65701 ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat cccacgactg
    65761 gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc ttattcttga
    65821 tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat cgtacctaat
    65881 tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat aagcgtcact
    65941 tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat acatacacgc
    66001 ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat ctttcgtggc
    66061 cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg agtgatatat
    66121 gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat caattcagaa
    66181 accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc agagggatta
    66241 agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc atgccataaa
    66301 gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt tttcagcact
    66361 tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc tgctgtagaa
    66421 aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa tgcagctgcc
    66481 tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc tgcttttaga
    66541 tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc tgcttgcaac
    66601 cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt cagtgcgtcg
    66661 taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct caactcgact
    66721 ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc tgcctcagct
    66781 gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa ttcttttcct
    66841 tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt atgtatcact
    66901 gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta gtcttccaaa
    66961 ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac attgccttca
    67021 tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc gacatcttga
    67081 aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt gcaagcgtaa
    67141 cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag tttcatttca
    67201 tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg agtgatccta
    67261 ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct tttcaataaa
    67321 tttagaacac tctcattcta ccaaattata accattttgc cacaaataag tccacaaata
    67381 acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta atcgttattt
    67441 gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc tttttggctg
    67501 ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc gccgtcacga
    67561 cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt tttctgcatt
    67621 ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc ttttgctgtc
    67681 gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa tttatctgct
    67741 gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg ttcaactgca
    67801 gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt
    67861 tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt aacgaaagtc
    67921 acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac gtagctttct
    67981 ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc taatccacgt
    68041 gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga acgcaatacg
    68101 ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta accgcgtgct
    68161 tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac gattgttaat
    68221 tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta ttcttttgcg
    68281 cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat cttcacatga
    68341 tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat cgttgctgag
    68401 aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat atcttccaag
    68461 aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac ggttccaagt
    68521 ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac aacgatatgt
    68581 ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta aacggcttgt
    68641 acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg gatagctaat
    68701 tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc gattttttct
    68761 aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg tccaataacg
    68821 tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa
    68881 ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt aaatttcaaa
    68941 tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc cgtcgatttg
    69001 ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta tccattctta
    69061 gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa aagccaaacg
    69121 ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt ttctatggca
    69181 aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata gtttttcgtt
    69241 ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac ttcaaataag
    69301 cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt ttgtttgata
    69361 gcatcaatca atttattctt ttctttttca aaatagaaca caggtagttg ggggtatttt
    69421 tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat catggcgaat
    69481 ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc tttccctaat
    69541 tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt tttaaaagta
    69601 tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa aatagctgca
    69661 ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg ggcgagccct
    69721 tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc aatcaaagca
    69781 ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc tactacaaaa
    69841 tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt tccaataccg
    69901 aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg
    69961 ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt ggctttagcg
    70021 attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt gattgctgca
    70081 gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc catgcccatt
    70141 tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata
    70201 ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa gacagacgcg
    70261 gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct
    70321 actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc tgctacagga
    70381 ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga agcagatgct
    70441 ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc aagaggaacg
    70501 aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat ctttgtcagt
    70561 gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt cattgagatc
    70621 tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg catgactctc
    70681 acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg ttacgcaatc
    70741 actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat tgacgctgat
    70801 ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat ttcaagccca
    70861 tattttccca taagagtggg tacatactga actccgtaaa tcctggcata gagtttaatt
    70921 cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc aatccgcttc
    70981 cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa acttcttctg
    71041 gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct tcataatcat
    71101 agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct tcatttccta
    71161 atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct cgagaatcat
    71221 actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct tttgtaatgc
    71281 cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa gaaccttcac
    71341 attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact ggtacataag
    71401 gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta tccatggcac
    71461 atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct aagaagcctt
    71521 ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg gctccttctt
    71581 ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg
    71641 ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt aatggacctt
    71701 tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa ttataataaa
    71761 tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc
    71821 catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt tcagtgattt
    71881 actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa aaaaacgagc
    71941 cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt gtcccgtttt
    72001 ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag
    72061 ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa actttgtaca
    72121 gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa
    72181 gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca ctcaaacatt
    72241 caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa agacgatcga
    72301 aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat cgccttttgc
    72361 ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt
    72421 taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac tatttgaacg
    72481 agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa aaggggtact
    72541 ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg
    72601 cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca agatttatga
    72661 cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca
    72721 tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa acaagcagct
    72781 gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt ttgatgatgg
    72841 ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa caaaagaaga
    72901 acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg gttcctttgc
    72961 ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca actacatcga
    73021 ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac aagccgtttt
    73081 agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca
    73141 agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc ttgaacagta
    73201 ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg tcgtgacgat
    73261 ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga aagcatttgg
    73321 gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc
    73381 gccttttgct caacattatt ttctcactca tctggatgta gaagatacat tgaaacaaag
    73441 cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac agctccacca
    73501 tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga aaaagcaaga
    73561 caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta tgattttagc
    73621 tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt
    73681 cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta tttttttggc
    73741 aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga
    73801 aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca aatttttgtg
    73861 aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa
    73921 aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa cacatttatc
    73981 ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt cgttacctac
    74041 tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga cgaaaagaca
    74101 tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct tttcttaact
    74161 ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca aattcttttc
    74221 cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa ccataatctt
    74281 gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc gcactatcag
    74341 ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc gtataatggt
    74401 ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt atgacatctt
    74461 cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc cttcttccat
    74521 aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca ttttgccaag
    74581 ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat caataataga
    74641 tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg
    74701 tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg atccattgga
    74761 atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac tacgtgccag
    74821 aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac caataaagaa
    74881 gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt caaaatccat
    74941 ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat aaaaatcgaa
    75001 agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa aaggaaattc
    75061 cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt ccattccttc
    75121 ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc ataaatagca
    75181 agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa caataagcca
    75241 aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt gatgtctttg
    75301 aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac gtaaaaaagt
    75361 gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat cgcttgatac
    75421 aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt gataaaacct
    75481 tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc tgcactcagc
    75541 cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa tattccaagc
    75601 aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact atctagacca
    75661 aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat
    75721 ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag ataaggaata
    75781 aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg cccaaatcgt
    75841 ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat ctgctcttcg
    75901 attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt aaaaaaaaat
    75961 acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact caagcagaca
    76021 agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga taaagataga
    76081 aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga gtccttctcg
    76141 acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc ttttttcatt
    76201 ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc tgtaatctca
    76261 aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac tcgcaaaaag
    76321 atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac tgaagaagtc
    76381 aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat ttttccattt
    76441 atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa aaagtaggac
    76501 acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt cagcatgtaa
    76561 tccacttgtt tttgaccaat gatcggcaca aaaacttccg gccatacttc ttgtatgact
    76621 gccatcagct catccagttc attttctgtt acttttattt tttccataca cccacgtcct
    76681 tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat catgctatac
    76741 acacccaccg accacggttt caagaaaatc acaatctgca taaattccct aaaagataat
    76801 ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag cagcaaggta
    76861 acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc aaatttctga
    76921 tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg gacaagcaaa
    76981 aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc aaatccattg
    77041 ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg aatgatcact
    77101 tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg ctggataaac
    77161 gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag aatcaacaca
    77221 aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa aactgatcct
    77281 tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc tcct
//
LOCUS       DAYYCQ010000004        60413 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000004 DAYYCQ010000000
VERSION     DAYYCQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.004"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1420)
                     /locus_tag="ACHAYF_000300"
     CDS             complement(<1..1420)
                     /locus_tag="ACHAYF_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM8577767.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1672..2853
                     /locus_tag="ACHAYF_000301"
     CDS             1672..2853
                     /locus_tag="ACHAYF_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8577768.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2913..4541)
                     /gene="treC"
                     /locus_tag="ACHAYF_000302"
     CDS             complement(2913..4541)
                     /gene="treC"
                     /locus_tag="ACHAYF_000302"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8577769.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4992..7703)
                     /locus_tag="ACHAYF_000303"
     CDS             complement(4992..7703)
                     /locus_tag="ACHAYF_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8577770.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7713..8414)
                     /locus_tag="ACHAYF_000304"
     CDS             complement(7713..8414)
                     /locus_tag="ACHAYF_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8577771.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8600..9199
                     /locus_tag="ACHAYF_000305"
     CDS             8600..9199
                     /locus_tag="ACHAYF_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8577772.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9280..10293
                     /locus_tag="ACHAYF_000306"
     CDS             9280..10293
                     /locus_tag="ACHAYF_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8577773.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10401..11318
                     /gene="rbsK"
                     /locus_tag="ACHAYF_000307"
     CDS             10401..11318
                     /gene="rbsK"
                     /locus_tag="ACHAYF_000307"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8577774.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11346..11741
                     /gene="rbsD"
                     /locus_tag="ACHAYF_000308"
     CDS             11346..11741
                     /gene="rbsD"
                     /locus_tag="ACHAYF_000308"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8577775.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11741..13114
                     /locus_tag="ACHAYF_000309"
     CDS             11741..13114
                     /locus_tag="ACHAYF_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8577776.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13471..15615
                     /locus_tag="ACHAYF_000310"
     CDS             13471..15615
                     /locus_tag="ACHAYF_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8577777.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15667..16707)
                     /locus_tag="ACHAYF_000311"
     CDS             complement(15667..16707)
                     /locus_tag="ACHAYF_000311"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8577778.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16984..18786)
                     /locus_tag="ACHAYF_000312"
     CDS             complement(16984..18786)
                     /locus_tag="ACHAYF_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8577779.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18800..19552)
                     /locus_tag="ACHAYF_000313"
     CDS             complement(18800..19552)
                     /locus_tag="ACHAYF_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8577780.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19561..19923)
                     /locus_tag="ACHAYF_000314"
     CDS             complement(19561..19923)
                     /locus_tag="ACHAYF_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8577781.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20099..20971)
                     /locus_tag="ACHAYF_000315"
     CDS             complement(20099..20971)
                     /locus_tag="ACHAYF_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8577782.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21022..21510)
                     /locus_tag="ACHAYF_000316"
     CDS             complement(21022..21510)
                     /locus_tag="ACHAYF_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8577783.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21648..22439)
                     /locus_tag="ACHAYF_000317"
     CDS             complement(21648..22439)
                     /locus_tag="ACHAYF_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8577784.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22494..23102)
                     /locus_tag="ACHAYF_000318"
     CDS             complement(22494..23102)
                     /locus_tag="ACHAYF_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8577785.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23095..23412)
                     /locus_tag="ACHAYF_000319"
     CDS             complement(23095..23412)
                     /locus_tag="ACHAYF_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8577786.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23568..24296)
                     /locus_tag="ACHAYF_000320"
     CDS             complement(23568..24296)
                     /locus_tag="ACHAYF_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8577787.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24455..25336
                     /locus_tag="ACHAYF_000321"
     CDS             24455..25336
                     /locus_tag="ACHAYF_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8577788.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25573..26616)
                     /locus_tag="ACHAYF_000322"
     CDS             complement(25573..26616)
                     /locus_tag="ACHAYF_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8577789.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26619..27287)
                     /locus_tag="ACHAYF_000323"
     CDS             complement(26619..27287)
                     /locus_tag="ACHAYF_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8577790.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27429..29765
                     /locus_tag="ACHAYF_000324"
     CDS             27429..29765
                     /locus_tag="ACHAYF_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8577791.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29880..30191)
                     /locus_tag="ACHAYF_000325"
     CDS             complement(29880..30191)
                     /locus_tag="ACHAYF_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577792.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30372..31847
                     /locus_tag="ACHAYF_000326"
     CDS             30372..31847
                     /locus_tag="ACHAYF_000326"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8577793.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31894..32478
                     /locus_tag="ACHAYF_000327"
     CDS             31894..32478
                     /locus_tag="ACHAYF_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8577794.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32480..32863
                     /locus_tag="ACHAYF_000328"
     CDS             32480..32863
                     /locus_tag="ACHAYF_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8577795.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33180..33464)
                     /locus_tag="ACHAYF_000329"
     CDS             complement(33180..33464)
                     /locus_tag="ACHAYF_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8577796.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33816..34526)
                     /locus_tag="ACHAYF_000330"
     CDS             complement(33816..34526)
                     /locus_tag="ACHAYF_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8577797.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34523..35995)
                     /locus_tag="ACHAYF_000331"
     CDS             complement(34523..35995)
                     /locus_tag="ACHAYF_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8577798.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36133..36606
                     /locus_tag="ACHAYF_000332"
     CDS             36133..36606
                     /locus_tag="ACHAYF_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8577799.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36759..37310)
                     /locus_tag="ACHAYF_000333"
     CDS             complement(36759..37310)
                     /locus_tag="ACHAYF_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8577800.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37415..37945
                     /locus_tag="ACHAYF_000334"
     CDS             37415..37945
                     /locus_tag="ACHAYF_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8577801.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38428..38871)
                     /locus_tag="ACHAYF_000335"
     CDS             complement(38428..38871)
                     /locus_tag="ACHAYF_000335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8577802.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38887..40047)
                     /locus_tag="ACHAYF_000336"
     CDS             complement(38887..40047)
                     /locus_tag="ACHAYF_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8577803.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40554..42254
                     /locus_tag="ACHAYF_000337"
     CDS             40554..42254
                     /locus_tag="ACHAYF_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577804.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42356..43189)
                     /locus_tag="ACHAYF_000338"
     CDS             complement(42356..43189)
                     /locus_tag="ACHAYF_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8577805.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43493..45658)
                     /locus_tag="ACHAYF_000339"
     CDS             complement(43493..45658)
                     /locus_tag="ACHAYF_000339"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8577806.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45918..48212
                     /locus_tag="ACHAYF_000340"
     CDS             45918..48212
                     /locus_tag="ACHAYF_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8577807.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48205..48870
                     /gene="pgmB"
                     /locus_tag="ACHAYF_000341"
     CDS             48205..48870
                     /gene="pgmB"
                     /locus_tag="ACHAYF_000341"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8577808.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48863..49870
                     /locus_tag="ACHAYF_000342"
     CDS             48863..49870
                     /locus_tag="ACHAYF_000342"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8577809.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49892..50911
                     /locus_tag="ACHAYF_000343"
     CDS             49892..50911
                     /locus_tag="ACHAYF_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8577810.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51192..52559)
                     /gene="nhaC"
                     /locus_tag="ACHAYF_000344"
     CDS             complement(51192..52559)
                     /gene="nhaC"
                     /locus_tag="ACHAYF_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8577811.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52668..54089)
                     /gene="tyrP"
                     /locus_tag="ACHAYF_000345"
     CDS             complement(52668..54089)
                     /gene="tyrP"
                     /locus_tag="ACHAYF_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8577812.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54332..56209)
                     /gene="tdc"
                     /locus_tag="ACHAYF_000346"
     CDS             complement(54332..56209)
                     /gene="tdc"
                     /locus_tag="ACHAYF_000346"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8577813.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56500..57756)
                     /gene="tyrS"
                     /locus_tag="ACHAYF_000347"
     CDS             complement(56500..57756)
                     /gene="tyrS"
                     /locus_tag="ACHAYF_000347"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8577814.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57868..58073)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58336..59637
                     /locus_tag="ACHAYF_000348"
     CDS             58336..59637
                     /locus_tag="ACHAYF_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8577815.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59706..60305)
                     /locus_tag="ACHAYF_000349"
     CDS             complement(59706..60305)
                     /locus_tag="ACHAYF_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8577816.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18239 a        12358 c        10827 g        18989 t
ORIGIN      
        1 ttcctgcata taaatgaaaa tgttcggagt cagccggtgc aatcgtgtga tcactgaatt
       61 gaatatatct aaaagcgcca ctttccttat cttctgcttc gaatagatac cttacgccac
      121 ggtttcctgc ctcgtaggtc aagatttctt tccctacata acgataattt gaacgtttcg
      181 ttgttccatt cttaaacgta aatgccatct ctttctcact gatcttgatt gaagcaatat
      241 ctgtttgata gcccttcgta taatattctt tatattcttg tgctgtctta tcctgattca
      301 atttttcttt atattcaaaa acaggatcaa aagtcccatc ttctacatac gtatctactg
      361 tttgccaagt tcctgcccaa tctgaaagag agcgatcttg tacatcctcg tcttcaaaat
      421 aaccgttgta gacgctcttt tcttttttgg gggtcaaagt atcttctagc gggttttctg
      481 tctctgtcgt ttttttcaac gcttcgaggt ttgcttccat gatagaaaga tagttttctc
      541 cattttctat ttgttcattc gtcaatcctt ccaaaggatt caatacttct aaagatacac
      601 ctgcttcgtt tgctaacgtt ctggcaatac tatctttggc gttttcttca aaataaatat
      661 agttgattcc atgttcttta acaaagtgtt tcagttcagc aattctcgcc gcagacggtt
      721 cttcactaga agaaagacca gcaatcgata cttgattcaa tccatattct aacgctagat
      781 agttgaatgc agcatgttgc gtgacaaaag atctttgttt tgcttgctta aaggtttctt
      841 gataaagctg atggagtgct tgaagtttct cggtataggc tgctgcattt tttgtccata
      901 cttcctgttt ttctgggtaa gcttcaatca gctgatcacg tatgtttgct acctgcttga
      961 ttgctaaact tggagctagc catacatgtg ggtctagctc atgtgtatgg ccttcttccc
     1021 cgtgttcatg gtcatgatca tgtccttctt cacttcccgg aagtaaaagc ataccttctg
     1081 tcgcttttat cgtatggaca ttgccttctt gtaatgtttt ttggattgcg gggacccatg
     1141 tttccatgtt ttcatcatta taaacaaaga tatctgtatc ttggatcttt gccatgtctt
     1201 tagcagaagg ttcataatcg tgggaatcag tccctgctgg aatcaataac tctacttctc
     1261 cttcatcccc gacgattgct tttgtaaaat catacatggg ataaaaggtc gtcatcacgc
     1321 tcaatttttc tttactgtcc tcggctttcc cacttgcccc gcaagcagtt aacatacctg
     1381 ctaaaaccag catggccaat atacctatca atcgtttcat ttatgacact cctcttctca
     1441 tcttctttca tttgaatgtt ccgattcgct tttcgcaaac cgtaatagtt acgttttagc
     1501 aacaggattt aatttatcat atggttaaaa gaactgtcaa gcgttttctt attttaattg
     1561 atgagaaagt ttatatgaat gattgacgat atttttgcaa tcaacctcta tcattttcct
     1621 tttatacccg cttttgtcta taatgaagtt atagataaag gaggaaaaag agtgaaaaaa
     1681 gaaacagata caacgaatcg tttgatccgc tttttaggag gaaaaacctc ttactatgtt
     1741 ttaggaatag ttatcttact tgcgctgatc atttattttt tccatcagat ctcgtttata
     1801 ttcaagcctg tcggaatcat tatcgctact atcttgcccc cattattgtt tgcactgatc
     1861 ttgtattatc ttttggaacc agtcatcaaa cgtttgagca agcacctttc gaggaacttg
     1921 tcagtcatta tcgtttatgt gattatttta tttttattgg ggcttggaag catatggctt
     1981 attccttttc ttgaagaaca ggcaaagaca ttgatcagta gtttgccaga tttgtttcaa
     2041 gattttcaag agagtctccg acaatttttg gagaaaacac cttttgccga ttcatttaat
     2101 caatttttcg cttctattga cgacgtgacg tctgatatag ctggatttat tggaaattat
     2161 tgggaaagtg gcgcacaacg gatcagcaac attttttcaa ctgttacagc tatttttatt
     2221 actttattta ctggtccaat tgtcgctttt ttccttttga gagatcctca aaaattttat
     2281 cagtcagttt tagcgattgt cccaccagca tttcgtacag attttaaaaa tttgacaaag
     2341 attgctaatc aacaattggg ttcatttcta aaggggcaaa tcattgcttc atttatcctt
     2401 ggagcagtct actgggtttg tttcttactg atcggattag aatttgctag tgttttagct
     2461 atttctgctg ggttgttatg tatcattcca tatattgggc cgtttatcgc cttctttcca
     2521 ggtttgatca ttgcattcca agactctact tttatggctg tcaaatttgt cattgtctgg
     2581 tttgccgtac aacttctcca cggtgacttg gtgattcctc gagtaatggg tgatcgttta
     2641 cagatccatc cgattacgat cttaatcgtt ttacttgtca tgggtgatct aatgggaatt
     2701 gtgggcgtta tttttggtat tcctatctat acgttagtca aattactcgt tacttttatt
     2761 ttccgtaaat tcaaacaacg ctacaataaa ttttatggcg ataaaggaga gtatgaaaac
     2821 tcttcatttt ctgaagaaga ttattttaaa taaaacacaa aacgtcgatc ttacgagaat
     2881 agctcgtagg aacgacgttt tgtgtttttg ttttaatttt tcagcaaagc aattgcctgg
     2941 tatggtttta gctccagact ttgatgaaga acagtatcgg aataattatc gataagaata
     3001 ttggcttctg tgaatccttc tgggatggcg atccttgtat ctttatcata gaaatttgtt
     3061 aatacaagca gctgctgtcc ttcaaattgg cgaagaaagc cataaacttc cggatgattt
     3121 gcttcataag catgataatc tccttgtgca acgattggat atgtttttct cagccgaatc
     3181 aatttcttgt aataggacaa aatagaatct tcagaagata gttctttttc tacattaatc
     3241 tcttctagct tgcctccaac ttttaaccaa ggtttccctg tagtaaatcc tgcttgttct
     3301 gtgtctgacc attgcatggg tgttcgagag ttatcgcgag attttgcttt gatccgacga
     3361 aacgcttctt tttctgtgaa tccttgttca aggagtaact tataagcatt caaactttct
     3421 acgtcaatat agtcggaaat cgatgaatag tctgggtcga tcatgccgat ttcttctccc
     3481 atataaatat agggcgtccc acggttcaaa tggatcatgg ttgctagcat ctctgctcct
     3541 tgttttcgat actgatcgat agctacaaat cgattgagcg cacgtggctg atcatgatta
     3601 ttaaaaaata gagcattcca gccgttaccc tcactcatct tttctcccca tgtatgaaat
     3661 agtcgcttta attctgcaaa atcaaatgga ggagtagacc atttattccc attttcataa
     3721 tcgactttca aatgatggaa attaaagacc attgataatt cttctctact aggttctgta
     3781 tacaagatac aattttcgat cgtcgtagag ctcatttcac cgactgtgat gatagtggaa
     3841 tccgtcccaa acgttgcttg gttcaattct tttagatacg tatgggtgat tggtttgtcc
     3901 gtatattcag gtttgccatc attttcttta ttatttttca attgttcgtc tttaccgata
     3961 acattgataa catcgaaacg gaatcctttt accccttttt ccatccaaaa acgaacaact
     4021 ttgaatagtt cctctcttac tgcttggttc cgccagttca agtcagcttg tgtccgatca
     4081 tataagtgca aataatattt ccccgtatct ccaaatggcg accaagcact tccaccaaat
     4141 tttgataccc aatccgttgg ctcttctctt aaaatgaaga agtcctgata atgctggtcc
     4201 cctgctagtg cttttttgaa ccattcatgt tctgtcgaaa cgtgattcag caccatatcg
     4261 agcatgattt ctatccggta tttctttgct tcggctatta gctgttcgaa ttcctccatc
     4321 gtaccaaaga taggatcgat ggctgtgtaa tcagagatat cataaccgtt gtctttttga
     4381 gggcttggat aaatagggtt tagccaaatc atgtcgatgc ctagatcatt cagatacggg
     4441 agtttttctt tgattccttt taaatctcca attccatcac cattactgtc tttgtaagat
     4501 ttgggataga tttggtaaat gactttgtct ttaaatgtca tggatattta cctccattca
     4561 actgatttat tacctaccac aacttatata tacaagttat catttattat tataactgaa
     4621 ccgaaagcgt ttgtaaaatt tatctttata cattctaaga ggtctgttgc ccaataaaaa
     4681 acaagagatc agacatgtat ttgtctatcc tcttgtttaa aatagcaaaa tattcttgtt
     4741 tcttctataa atggtgttca aaagctttta tctacggcaa tcagtcttct caaatgtaaa
     4801 acatgaggag ctaattttat ttgagtattc tttttgctcg atgcaggacg gcttttttac
     4861 aacctctgat tcatggtgtt caatcagctg tccttcggta ttagctcaaa aaacggcaaa
     4921 atatgaaaaa ctattttgcc gtttgtttct ctaatactca ggtcaaaccg ctgatttcac
     4981 aacctctgtt tttattcatt cgatttcaac gcatcaatca tatcgatatg ctttaatttg
     5041 aaatgggtaa caatcatcac aataaccgta aatacgatag ttaatcctgc tgataagaca
     5101 taagcccacc atgtgatcac taatggaaag atgacattct ccatagacgc ttgttgcaag
     5161 atgaagtcag tcaatatata tcctacacca aatcctccga tgattcctaa aagagtaaag
     5221 atgatatttt ctcgaacaat gtacatcgtg acttctttat caaaaaatcc aaggactttt
     5281 attgtagata attcgcgtac acgctcggaa atattgatat tagttagatt atacaagacg
     5341 ataaaagcca gcagtcctga taatacgaca aagattaaga caaccgagtc taagttcgcc
     5401 attgattctt cttgcgtttc gatttgtttg gacatgaaag acgtatttgt cacagcgtct
     5461 gtggcaagca gtttctcgga aagcatttct tcttcttttg ttgtcatctt atctgtacgc
     5521 agcaaaaatg cattatccat catcgattga tcagtaactg aagcataata ttcaggggac
     5581 atataaataa agctacctag atagtttttc acgatttctt ttacttcgac tttccattct
     5641 ttttgatctt catcataaag cgaaatatga tctcctgtag ataagtcaaa tagttctgct
     5701 actttttgcg taatagcgat tcctgtttta ggcaaggagc gtgggttctc ttcaagatca
     5761 gttaaatgga taaaacgatc caaatcagct gttttacttg gtacgatcac tgtcagactt
     5821 tgcgaagcat gtcctttttt tctcaattct acggtttgag tatgaatagc taggatctca
     5881 tttacttttt cttgcttctg cagcatatcc gctgcttttt gacgactatc ttccgtggat
     5941 gaatccaatg taacgattgc ttgatagtca atgatcggtc caaattgttt ggctgaaaca
     6001 gaacttagag aatctttcaa tcccacgcct gcaaccatca gcccggcaca tccagcaatt
     6061 ccgataatcg ccataagcat tcttgactta tatcggaaga gatttcggta actgattttt
     6121 tgattaaagc taaggcgtga ccacagcgga gtgatgtatt caagaaaaat gcgtttccct
     6181 ggcttcggtg ctttcggttg aagcaatgca gcaggccgtt ctcttaaatc tttgaataaa
     6241 acgatcattg cagcgccaaa tgcggcaatg aagaatgcgg cagctgcttg taaaatcggc
     6301 ggccagtcat aaaaaataag cgcactccca atatcatacc gctcgttaga cagctcaaaa
     6361 atgatccttg gcaaaagatt cgttcctaat atcgttccta aaacaatacc tatccctgag
     6421 gctagacttg catagattgc atattttttt gcgatttcaa ctttcgtata acctaacgcc
     6481 tttaatgtcc cgatttccct gcggttttct tctaccatac gtgtcattgt cgtaaaggtg
     6541 atcaatgcgg caataaagaa aaagaaaaca ggaaaaacat tggcaatcgc agcgatacga
     6601 tcagacagat caccaaattc ttgaaaaccg acattttctg aacgttcatt gtataggtat
     6661 gatggtttag ttaaattttc aattttttct gcatttcgat tcaattcttc ctctgcagag
     6721 gccagttcac gttctttctc aactaattgc ctttcttgtt cagcaagttg agcttcttgt
     6781 tgagataatg tttgaacagc gattgttcgt tgcgcttcag gaagctgctg gatagttgct
     6841 gactgttgcg ctaactgttt tttagcatct tctaaccgtg ttcttgcttg cgttgtttgt
     6901 gctttgccat ccgctatttt ttgctgtgcc ggttctaatt cttcgttggc cgattttttc
     6961 agctcttcta agcgttcttc gggtcgatct tgtaaatact tttctgcctt ttcttgattt
     7021 tgctccattt tatctttgta agctttgctg tacgtattca aattctttac gtttgtaaaa
     7081 tcaagatata agacagaata aatatctgtt ttaaaaacgg attcaggtaa atagacaaaa
     7141 taatcgacgc taccatttcc gacgttcgcg tatcctctct ccaaattgtt gatgaacaat
     7201 ggagaacgga caaagcccgt gatggtatac tctttttctt tcaattcatc tgaatcaatc
     7261 tgataaatat caccgatttt atacccttca tcttccgctt tttcatccaa tacaagctgg
     7321 tttggttttt ccgggagttt tccatcagtt aattgtagct tgttctgctt gttatttttg
     7381 ttataagaaa atatttgaat cacttcgttt ttgccagtat tggaataata gatctgtttt
     7441 cctgtttcta cttttgcatc tgggatcttt tctgcttgag caatatcttt ttgtgtcaag
     7501 ccaccagtag aaacgatttg caaatcagaa agtcctgctg taccaacata gtgatccatc
     7561 gttttttgca tgacaggtcc agcagttttg acacctacaa ataaaagtgt tcctagcatg
     7621 acaataagga tgattgcaat gaatcgtcct ttagattgcc gaatttcgcg aaaacctgct
     7681 ttaagatatg ttcttgtttt cataaaatcc ccctaccata caatctcatc aatggatgaa
     7741 ggatgatcat ttaattcaac agaccttact ttagcatcat taatgtgaat gactctatca
     7801 gcgatgggcg ctaaagcaga attatgtgta atgatcaaaa cggtattgcc gtgttgtctg
     7861 ctagcgtttt gcaataattt caatacttgc ttgcccgttt caaaatccag cgcgcctgtc
     7921 ggttcatcac ataaaagaag tttgggattt ttagctagtg cacgagcaat tgaaaccctc
     7981 tgctgttcgc ctcctgataa ttgggacgga aagttattca atcgatgagc taggcccact
     8041 tgcctcaata cctctacagg atcaagcgca tcaggagaaa cttccgttgc caattccaca
     8101 ttttcttttg ctgttagatt aggaacgaga ttgtagaatt ggaaaacgaa ccctacatct
     8161 gttcgtcggt aagcagtaag ctgtttatct gaaaattgcg caatatctgt gtcatcaata
     8221 atgatctgac cttcatcagg tgtatccatc ccacctaaaa tattaagaat cgttgacttt
     8281 cctgctccac ttggaccaag tataatcact aattcgcctt tttcaattga aaaagaaata
     8341 tcgtcatttg ctgtaatggt cgtttctccc attttgtatc gtttgtattc attttttact
     8401 tcgacatact tcactctgat tcctccattt tcacgatatt tctcgctata tctatgcatc
     8461 gaatgcgttt tctcacactt atttatttta ccactgaatt tctctagact aaagaaagga
     8521 atcctttaat ttagttgaca aataaacgat ttatgattta cttacataaa aaacattgtt
     8581 atttgaaaga aggaacatca tgaatgattt aaaggaaaat atgatttttg atttatcaaa
     8641 aactgctttt gtcgcgatcg acatgcaaaa agagatcgta aataacggta cactctctcc
     8701 acacactgca gcatctattc tgacaaacaa tgatttgtta gtaagaacat tgaaacgtac
     8761 cgctgccttg aaagttttag tgaacgtaga catctccact ttcccttatt tgagtcaaca
     8821 aactgatatg ggtggaatga ctgcttctgt acctccagaa ttcactgatt tacttttgaa
     8881 ggattctttg aaagatacag ataatatgtt gactatcaca aaatacaatc caagtgcttt
     8941 ttttggtact tctcttgatc ttcaacttcg ccggcgtgga atcgaaacta tcattttaag
     9001 cggtgtagcg acaacaaacg gtgtatatgc tactgcatta gatgcctttc aacacgggta
     9061 ccatatcgtg cttgctgaag atgcttgcag tgatcgtgac aaagaatccc atcaattgtt
     9121 cataaaaaag atatttccga aaactgcacg agtacgctca acgaaacaaa taatcgaagc
     9181 tatccaacaa agcaaataag tactggcaca cgtgattttc atttacttct ttttgatttc
     9241 atgggaaaat aaccacagga tttagatagg agtatgaaca tggtgaaaaa aaagatcaca
     9301 atcaaagaag tcgctaaact ttccggcgtt tcaatcacaa ctgtctcctt gatattaaat
     9361 ggaaatacgg agaaattcag tccgaaaact gttcagaaag ttttagctgc caaagaagaa
     9421 ttaggctatc agcctgacta ttttgctaga caaatgatta ccaaggaaac gaaaacaatt
     9481 ggtgtactcg ttcctgatat cacgaatcca ttcttcagta cactgatgcg aggaattgag
     9541 gatatcttgt ataagcagaa tttcgtcacg atattatgta atgctgattc tgatcatcaa
     9601 aaagaaatcg aatatctagc cgagctcaca cgtcgaggtg tggatggatt cattatcgcg
     9661 acttctgctg tctctacaga tgcaatcaac gaaaatttga aaaaacaagg ccggcccttc
     9721 attgttttgg atcagaaaaa atctgaagga tttagtgatg ccgtacgaac agacgatttt
     9781 cgcggtggtt acctagcggg catgcacttg cttagtctag gacatcaaac gatcgccctt
     9841 gtctatcctg aaaatccacc ggaaaatgtc cacgctcgga ttgaggggtt taaatctgct
     9901 cttgatgttt accaaatccc tcacgaccaa ctgatcctct tacctacaca gttctcaaaa
     9961 caaggtggct accagatcac agcagaactt cttgattctg ctgcaactgg tgtgtttgct
    10021 ttaaatgatg aattagcttt tggattatat cgcggattag aggaagctgg aaaaagcatc
    10081 cctgaagact acagcattat tggctatgat aatatagata tgtgcgagta tatcaagccg
    10141 aaactgacaa cgatcgcaca accaatcttc gaattgggac aaacatctgc caagttattg
    10201 ttagatcgga tccaattccc agaaaaagaa tgggaagaaa aaagattacc tgtacgatta
    10261 gaaaaaagat tttcaactgc acccttgaaa tagctttcaa ggtgtgctat atttttatcg
    10321 aactattaaa acgttttact aaaacgtttt aaaaaataat tttcccatca aaataaatga
    10381 gatagttgga ggtttgtaaa atgaacacgg ttacagtaat tggaagtatt aatatggaca
    10441 ctacccttcg tttaaccaac atgcccaagc caggcgaaac gatgcatgcg catgagattt
    10501 ttcacgctgg cggaggtaaa ggtgccaacc aagcagtggc agccaaacgt tcaggcgcac
    10561 ggacatcttt tattggaggc gttggggctg acagcgaagg acaacagtta ttagatctac
    10621 tgacaaaaga aaacatcgat acatctggga tagctgaaat ccaaggcgcc acaaccggac
    10681 aagcaatgat tatggtagac gcagcaggag aaaatagtat cttgatccat gcaggggcaa
    10741 ataatgcttt ccatgaacaa gaagtcctaa aaaacaagca actgattaca aacagtgact
    10801 ttattattgc tcagtttgaa agtagcttag atgcgacgat cctagctttt tctattgcaa
    10861 aagacgcagg aaaaacaacg atcctgaatc ctgcaccagc tcgtgaaacg attcctactg
    10921 aactacttga aaagacagat atcattatac caaatgaaac agaaacagaa atcattacag
    10981 gaatcagagt gacagatcat aacagtttgg tcgcagcagc cgaaaaactc catgaattag
    11041 gtattggaac agtaatcatt acactaggca gtgccggcgc tttctatcat acagaaaaag
    11101 aacacggtat cgtccctgct tttaaagtgg atgccgttga cacaacagcc gcaggtgata
    11161 catttattgg cgcattaagc agcacacttc agccagacct tagtaacttg aaagaagcga
    11221 tcctttatgg aaatctagcc tcttctgttg ctgtgcagtc ttatggtgcg caaccttcta
    11281 tcccttaccg cgaagcttta gaacaatcag cagtctaaaa ttaacaatga gaggaagaaa
    11341 caaacatgaa aaaaggaaaa gtaatcaatt cagatatttc acgagtaatc gcacaaatgg
    11401 ggcactttga taaattaagt atcggggatg caggcatgcc tgtccccttt ggtactgaaa
    11461 aaatagattt ggctgtctca aacggtcttc caagtttttt atctgtttta gaaaacgttt
    11521 tagaagaact tgaggtacaa tcgattgaat tggcggaaga aataaagaca atgaacccgg
    11581 aaatattgga aaaaatcaaa ttattgttac cagatacacc gattagcttt atcccacatg
    11641 aagaaatgaa aaaagagttg aataactgtc atgcatttat tcgtacaggc gaaatgacac
    11701 cttacgccaa tattatatta gtgagtggag tggtcttcta atggaaacga caatgaatcc
    11761 tgaacagatg gtactgtccg agcagattca tatgctgatt gaacagttgg gaggaaaaga
    11821 caatattgat tctgtccttc catgtgcgac tcggcttcgt atcaaattga aaaaacagcc
    11881 agtagccaat gcagaaaaat taaaacagca aatcccttcc attaagtatt tcattttact
    11941 cgatagatat gcagaaataa tcatggatca gggattggct cctgcttaca aggaattgac
    12001 gcgtcaaatc gatcattcag ctccgaaaga aaagctaaat ccgatcgaaa aactatttgg
    12061 tttgatatcg gaaatcttta cgccgatcat tccgctgttt gccggttctg gtatattaaa
    12121 aggtctggtt gttttagcga ctacgattgg ctggcttagt gaaacaagcg gaacttatca
    12181 tattttgtct gctgcgtcga atgccgtctt ttattttctg ccaatctttc ttgctttttc
    12241 tgctgccaaa acgtttaaag tgaatggata tattgcggct gtcattgctg cagcactgat
    12301 tgaaccgaat ctgacctctt tactttcaga cagtggcaaa gcagctactg attttctggg
    12361 gattcctgta gtactgatgc agtatacgtc tactgtcatg ccggcgattt taggtatttt
    12421 cttttattct tttgttgaaa aattcctcaa aaaatatatt cgcgaaaata tgcaattagt
    12481 atttgtacct ttactttcat tagttatcac ggttcctttg acgttaatgg tattcgggcc
    12541 tgcgggtgtt taccttggcg aaggtttagc tgccgcaatt acttggatga tggatattaa
    12601 cggaccgatc agtggcgcaa tcatcgctgg tggttggaat attttggtaa tatttggtat
    12661 ccaatgggct gtaaaccctg tcatgatcag taatatctct gcttatgggt tcgaccgaat
    12721 cgttccgttg acaggagcag ccaattttgg aatggctggt gcagctttag gtgtatttct
    12781 gcgaagtaaa cgttcaaaaa cccgttcgat ctccggttct gcttttgctt cgattttact
    12841 tgcgggagtg accgaaccaa ctgtttacgg aattgcgatc ccattgaaaa aaccatttgt
    12901 tgctgcttgc attggtgcag ctgctggtgg agctgtaatg ggatttgccc aagtgaaagc
    12961 gatcgctttt gtatttggta gtctgaccac attgcctgca ttcatttccg gtactttttt
    13021 ctggtactta gcaggactgg ctgttagtct agtcgtagca atgatcacta ctctcgttag
    13081 cgggtttgat gaagacctaa tgtcttatga atagttaatg aaaaagactg aagtatgaga
    13141 tgtttatcac atatttcagt ctttttcttt ttaggatcat tatttttata aatttttgtg
    13201 atactttcca aactttggta taatttacat atgcataacc atttttatcc ccacttgttt
    13261 atccccaaac aaaggtggtc tatgcaataa aatgaacgtc aaagaaaaac gaatgaatgc
    13321 tcgttgctta tttacaaatt gttttattta gaatacacag acagatttat gaaaagaaaa
    13381 agcttttcga cacgattatc tgttccaaaa aatgtctaat gaatgctgaa taagacagaa
    13441 aatcaatgaa aggaaaagga ctagtttatt atgaaaaata aaaaaacgat acgtttgcta
    13501 gctgctactt ggctgattgc tccgtctgtt ttgtcgacca ataccgtatt tgccgtagat
    13561 aatcaaatag ctgatcaaac agtcacaaca gatacgacta catccagctc agctactagt
    13621 gaaacaacgg attcttttac agaagaccca actggaacaa ctaactcaac aacaggatcg
    13681 accgagccaa ctgaaattga tccggctgat ccatcagaaa atgaaaagcc agccgagaca
    13741 tattccctta caattgctcc ttctttgatt ggtaagatcc aactgactgc ttcgacagcg
    13801 gattcccctg aacagcaacc tttggatatt acggaaagtg gaacggtaga aggtttatta
    13861 gcggaaacga aggtaactta cactattacg ccagctgaag gtgaacaaat tgcttcagtc
    13921 gctgttaatg gtgccaaaga agtaaattat gatgcaaaga cctttacagt aggtacgaac
    13981 aatttatcta ttactgcaga atttaaagct ggaccggata cttcaacaga gaaaccaact
    14041 gatcctagtg aagaagtccc tagtacacca gatccggaaa cgccaaacaa accagatgtg
    14101 ggtaataaca acaataataa tggttcaaat aattcaaaca ataataacaa taattcgaac
    14161 agcgggaatt cttcgaatca aacaaaccca tctggatcga ataacaatgg aaatactcag
    14221 agagaaccaa accatccaaa caatggttca tcgaatggcg gaacgaacaa tgcttcatct
    14281 cgtccaaata cagcaaatca aagcggtata gagaatccat cttctgcttc ttctgatttt
    14341 gttgtaaaat caccaattga tgctgtattg cctacaaata caacgaatgt ccaacaagca
    14401 atcgttcgag aagcattcaa gcatcttgga aaaccatatg tatggggagc aaaaggtcca
    14461 aatacttttg actgttctgg tttgacttat tacgtatata tgaaagcaac cggtcattat
    14521 atcggtggct ggacaggcga acagcaatat gcaggtacgc agattccagt aagtcaggcc
    14581 caacctggcg acctcgtctt ctggggacct tcttccggtg ttactcacca tgtaggtatc
    14641 tatattggga acggacaatt tatccatgcg cctcaaccag gagataaagt tcgagtaact
    14701 tctatttcag atttcacgcc tgattttgct gtccgagtaa acttagcagg tttacctgct
    14761 gcttcaggtt cgctgacagg cggctcatct attttagatg gattagatgg ctcgttccat
    14821 ttcagtcaaa accaaacaac tgatcagttc ttgaaaaaga ttgcagatga tgcacaggaa
    14881 atcgggcaaa aagaaggaat ctacgcatca gtcatgatgg ctcaagctat ccttgaaagt
    14941 ggatctggaa acagcttatt atcaagcgaa ccaaatcata acctttttgg tatcaaagga
    15001 tcatataaag gaagcagcgt gaccttcaat acacttgaac aagatagttc cggacaaagc
    15061 taccaaatcc gcgcacaatt ccgtaaatat ccttcttaca aggaaagttt ggaagactat
    15121 gccgacttga tcaaaaatgg actgactggc aatcctgatt tctacaaacc aacttggaag
    15181 tcggaaacaa aagactacaa agaagcaacc aaatatttgg aaggacgtta tgctacagat
    15241 cgccaatatt cacaaaaatt gaacgcgatc atcgaagcgt acgacctgac aaaatatgat
    15301 gaaccgaaaa aagaagcagc aacaactgaa gaagcaaacg ctgaaatcct caatatgtct
    15361 aagcgattcg atgtgccaat caaatggaca acagatggcg tgccaatcac aggattagaa
    15421 ttttcaacga taaaacgcgt caacaacgct tcttccatct tccgtgtcgc ttcaatctgg
    15481 gatttgtgga acaattttac ggcaagacag ataccagaaa caactactcg tacagttaaa
    15541 gctgcaactg ttcctgtact aaccttgtta aggttagaca aatcattctg gcttgatctg
    15601 cctttaaaga aatagaataa attaaaaagg ggatgtgaca ctgtcacact ccctttttaa
    15661 tttattctat ttctttatat ttattttgta ttccgttaca aactcgcgta cttggttagg
    15721 ttccagagtg acatctccaa aactttcaag tcgctccgct cctggcagtt cttgagtctc
    15781 aaatgtgata ccgccatgat ttactaattt attcccgcgc atttccggtc catcttctcc
    15841 aaaatttgct gtaaaaatca cgattcctga agcatctgtt tttacttgga tcgatacttt
    15901 ttcatctggg cttgtcaatt ccgcagcatt tagtaaacca gattgttttt tcaacaaaaa
    15961 tggatgatcg atgccgccca ctatcattga ttggcgaaaa tcactggcaa agacctcacc
    16021 taatttttta ggacgttgga agtcaaatgc tgtgccactt actgaaacta cttctcctgt
    16081 aggcgttgta tctttattga gcgtagcaaa taatcggcta ttcaaccaaa gtatatgttc
    16141 gtcgattgac tgtgctggat caccggttaa attgaaatag acatgatttg taggattgaa
    16201 tactgttttg cggtcactga tcccttggac cgtcactcgc catgtatcat cgttcgtcag
    16261 cgtataccgt acttctacat ctaaagttcc aggaaatccg ttttccatat ctgggctagt
    16321 ctgatagaaa atgaccgatg cttcgttttc accattgatg actgtataat gccactgctt
    16381 ggcttcaaag ctaggtgcac caccatgtaa acaatgacca gtgtccgggt ctgtttttac
    16441 ttcgaacgtt tcatcatcta atttgaatgt gccattcgta atccgtccag ccatacgtcc
    16501 gattgaagca ccgatataag ggtctttttc tagatattct tttgcttcat caaaacctaa
    16561 gatcaactct cgtttttcat tatcgattat tgtttgaaaa tgaacaaccc gtgccccaag
    16621 atcactgaca gacaaagatg tcccattttg attcgtgatc gtaatcaagt gatagttttc
    16681 gccaaaattt tgttcattga tttccatgtt ccttgcaccc tcttttcttt catttgcact
    16741 gattccagta tacatggaaa ccgttttctt aacagcttat tccgtgagta acgagaaaaa
    16801 atttactata ttctaataaa agaccgtgaa aaaaaggttg tgacaaaaat attgtcacaa
    16861 cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa
    16921 acgaaaaata tgaggagcta tttttgcttg gctgttctta ttacttgatg caggacagct
    16981 ttttcacaat ctctttttca atacgattcc agtatataac catgtcgtca gtacatagta
    17041 gatgaaatac aatacgaaga atatcgtaaa cggaacccaa atatctttgt atggattttg
    17101 catgaataga ccaaacattt gtaatccgaa taagacatgg actacaccta agattcctgg
    17161 tgctaagaat aacacgccta cttctctacg gatcgattgt ttaagcaacc cgcgtcgtgt
    17221 tcctatcttt tcaagcattt cataacgagc aatgtcactc ttgcttcctg acaagatctt
    17281 gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa cctaggaaga atcccataaa
    17341 ttcaaaacca gagaatagac cattatacac ttcatacatt tcatatttct ggttgctgta
    17401 ctctactttt tgcaaagatg gattgttcat tttattttga gcaacaagtg tttcgattgg
    17461 tttcaggttt gcttggaagt cttttacctg aactagctgt aaagtggatt gtggcaagtt
    17521 cagttgatca aattctgatt gactgactac cttgatttct ttttcttgct gttccggcaa
    17581 ttcatagctt cgaagttcct cttgtacagt cagttcattc atttttttag caggaacttt
    17641 ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt atcatgatca atggttgagc
    17701 cgcaaattca cttgcattat agtaaatcgt tcccgcatct tctttttgag tataagaagc
    17761 agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct tctgcccgag gattattcat
    17821 cactagatca tatccagaat tgttttctgt caagtctgca atttcatttt taaaacctaa
    17881 tcctaccgtc aaagcgccca gtgccaaggc aaacagcata gcgaccattg agagcatttg
    17941 tgtatattca cgaatacgaa agtttaactg tgacaatgtg aaattgttca attttttcaa
    18001 tgaaatctgt tctgatttct ttagtaatga taaaaagaaa atgatcacag aatggaaaat
    18061 gaaataagtt cctaatacga tcgtaactaa ggctacccct ataccaaaga cttgataggt
    18121 ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa gcaattccca gtactacttc
    18181 aaggaaaaac aagaatttat gctgcttgat tcttgttggt gtctgatccg cacgaatcaa
    18241 tgctaaaatc ggttttttga caatcgaaaa agcattgaca acagcagcta ataaaaacaa
    18301 aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata ctaaaaggtg taaaatgcga
    18361 aatattgaga tctaactgat ccactaataa tcggtggacg atacttgtca atcctacacc
    18421 gacagctgaa cccactaaag tagctgcgat ccctactaag aatgtttcag aaaaaatcat
    18481 ctgtgcgatt tttctgcctt ttgctcccag catcataaac attgcatagt ctttttgccg
    18541 catcgtcatc aaaaaggaat ttgcatataa aatataaacg aatgtgatga tccctagtaa
    18601 aacagaaccg aatcggaaaa tgataactgt agccgcaacg atcgtattac ttttcaaaaa
    18661 agcttcgttg cttgccattg attcaaacat gtaaaaaata gcagatgcca taactaatcc
    18721 tgagaataac accaagtagt ctcttaaacg gctcttgatc ccagtaagtg ataatttcca
    18781 aagcatagtc aatctccccc tactgttcca agtttccaag aatagatagt atttctcgat
    18841 agaaggaatc acggctttga tctccacgtt tcacttcttg atggataccg ccatccttga
    18901 tgaataaaat acgttggcaa taacttgcag aaaacggatc atgcgtcaca agaagaatcg
    18961 atacatgatc ttttgtattc aattcgtcca tcgtatctaa aagatcccgg gcacttttgg
    19021 aatccagcgc acctgtaggc tcatccccta atagaatcgt tggttgtgtg atcaatgcac
    19081 gtgcggcggc gacccgttgt ttttgtccac cggaaatctc agaaggatag ctttccaaaa
    19141 tatgagagat ggataaacgt tctgcaattt tttggacacg ttgcttgatt tcttttggtc
    19201 tgacgccttg taaagaaaga ggaacagcga tattttcttg tgctgtcaag ctttctaata
    19261 aattgaaatc ttggaaaata aagccaatct cattcgcacg aaaatctgct agttgatttc
    19321 cttttaatgt ggtcacatct ttttgattga ttcggatatg cccatctgtt ggtttatcta
    19381 atgtggataa aatattcaaa agtgtcgact taccagaacc agaagcgccc atgatcccaa
    19441 taaattcgcc tttttctaca tcgaagctga tgccgcttaa tgcttgcgtc tttttctcat
    19501 tgttttttcc gtatactttt gttacattct gtacttcgac tattttgttc atttctttcc
    19561 ctacccttta attagttttt ccagtgctgt tttcggcacg tctttttttt cgacttcttc
    19621 atacgaagtg acacctttat ttttgttcca ggtgattttt aaatatgctc ctttacgaag
    19681 cggtcgagtc aattgtccct tgaagtccag ttctttttcc ttaccctctt tcgtatagcc
    19741 aggcaggata tatctatagt tgactgtttc ttgtccagag tctgattttg aaactgtttt
    19801 tgtaccatct gtcgtaattt gtacatagta ttcttctcca cccataacta cgtaatctgc
    19861 gattttcacc cctgcaagta ccacaacgat gaaaacaagt agaccgatca gaagtttttt
    19921 cactttttcc ccttctctct tactttatat actgattata gaaaaaagcg aacaattctg
    19981 ccttcgaatc aactatcatt cttctttcaa ttttgtcatt ttctttaaaa caactaaaaa
    20041 aagagttcaa gacaataatc cgtcctaaac tccttcttcc tatcttacaa aaagtatgtc
    20101 acttacggtc tttcatgtcg cctgtattga taacaggttg cgttcctttg tctgtaatga
    20161 ttgagacaag taaattattg atcaattgga ctttccgatc atcggtgaaa ttgatttctt
    20221 gtccatcttc gatttgctct aaggccattt gagtgattgt gacagctcct tcaacaatgg
    20281 tttgtcgagc agacaaaatg gcttttgctt gttgacgttg gagcatggca ctagcgattt
    20341 ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac ttcgacacct gctactgcta
    20401 aacgctcttg cagctctttt tgcaactcgt cagacactgc cgtcgtgttt cctcgaagcg
    20461 tcaaatcatt ctcattgaat gtatcatacg ggtattggct agcgatatgc cgaatggctg
    20521 tctcactttg gatttctaca aaatcttgat aatacgctac atcgaataat gcctttgctg
    20581 tatcaacgac tttaaagact actactgctg agatttcgat ggggtttcca tccaaatcat
    20641 tgacttttaa aacagaacta ttgaaattcc gaactttcaa cgaaacagtc attttttgtg
    20701 ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt acctaaatat tgtccaaaaa
    20761 acaaaattac ttttgcttga ttaggactga ctacagttgc cgaacttaac aacaataacg
    20821 tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac tttcgcttgc cacataccta
    20881 aatagaagaa aaacaatcca actaaagcta atacgaccaa tccgacaatc cctgcataac
    20941 cattgatata aaatgctctt ttttctctca tgctacttcc tgcgctcctt tatccttcaa
    21001 taaatatcta atgttacttt tttaattttt ttttcgaata cgcgccatta caccttctag
    21061 ttctaactct ttgtcttcag tgatgtacca accgcttgcc tgtgtccctc cggctgcctg
    21121 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21181 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21241 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21301 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21361 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21421 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21481 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21541 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21601 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21661 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21721 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21781 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21841 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21901 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    21961 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22021 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22081 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22141 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22201 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22261 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22321 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22381 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22441 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22501 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22561 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22621 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22681 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22741 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22801 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22861 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22921 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    22981 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23041 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23101 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23161 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23221 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23281 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23341 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23401 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23461 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23521 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23581 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23641 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23701 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23761 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23821 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23881 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    23941 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24001 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24061 atcccattac ctaaatgaac aatcaaacca atcgctgttc ctatcactaa agcaaaaata
    24121 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24181 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24241 ccaattagtc cgccaaacaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24301 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24361 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24421 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24481 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24541 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24601 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24661 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24721 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24781 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24841 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaag
    24901 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    24961 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25021 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25081 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25141 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25201 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25261 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25321 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25381 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25441 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25501 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25561 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25621 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25681 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25741 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagta caatctctgt
    25801 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25861 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25921 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgaa aaaaaacgat
    25981 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26041 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26101 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26161 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26221 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26281 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26341 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26401 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26461 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26521 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26581 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26641 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26701 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26761 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26821 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26881 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    26941 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27001 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27061 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27121 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27181 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27241 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27301 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27361 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27421 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27481 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27541 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27601 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27661 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27721 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27781 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacaaatcat atgcataaaa
    27841 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27901 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    27961 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28021 ataatcctga actggcccat caatacgcag atcgaatcat tgaattttct gatggtaaga
    28081 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28141 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28201 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28261 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28321 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28381 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28441 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28501 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28561 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28621 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28681 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28741 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28801 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28861 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28921 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    28981 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29041 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29101 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29161 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29221 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29281 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29341 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29401 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29461 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29521 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29581 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29641 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29701 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29761 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29821 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29881 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    29941 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30001 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30061 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30121 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactcctcc
    30181 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30241 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30301 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30361 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30421 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30481 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30541 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30601 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30661 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30721 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30781 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30841 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30901 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    30961 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31021 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31081 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31141 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31201 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31261 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31321 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31381 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31441 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31501 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31561 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31621 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31681 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31741 taagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31801 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31861 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31921 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    31981 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32041 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32101 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32161 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32221 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32281 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32341 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32401 gttaatcacc atacaatgaa acttttggat caatttgaaa ttgtaaatga gcattcaaaa
    32461 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32521 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32581 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32641 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32701 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32761 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32821 gattttagat tggttggaaa aaatagattt ccatatcaaa taaccagttt attataaaga
    32881 tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    32941 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33001 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33061 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33121 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33181 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33241 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33301 ttcacgatac tgccttcata cggcacattg tcaaaagttg cttggacttt aactcttcca
    33361 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgccgact
    33421 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33481 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33541 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33601 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33661 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33721 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33781 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33841 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33901 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    33961 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34021 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34081 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34141 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34201 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34261 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34321 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34381 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34441 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34501 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34561 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34621 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34681 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34741 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34801 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34861 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34921 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    34981 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35041 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35101 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35161 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35221 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35281 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35341 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35401 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35461 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35521 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35581 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacac
    35641 cgcaagcttg gatcaaacga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35701 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35761 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35821 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35881 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    35941 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36001 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36061 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36121 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36181 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36241 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36301 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36361 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36421 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36481 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36541 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36601 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36661 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36721 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36781 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36841 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36901 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    36961 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37021 ttaaccggta gttcattggt gggtgtatga atccactctg ccaattgttc cttcccttct
    37081 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37141 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37201 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37261 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37321 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37381 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37441 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37501 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37561 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37621 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37681 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37741 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37801 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37861 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37921 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    37981 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38041 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38101 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38161 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38221 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38281 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38341 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38401 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38461 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38521 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38581 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38641 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38701 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38761 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38821 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38881 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    38941 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39001 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39061 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39121 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39181 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39241 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39301 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39361 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39421 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39481 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39541 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39601 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39661 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39721 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39781 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39841 gtttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39901 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    39961 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40021 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40081 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40141 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40201 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40261 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aatagcttct catattttct
    40321 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40381 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40441 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40501 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40561 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40621 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40681 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40741 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40801 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40861 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40921 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    40981 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41041 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41101 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41161 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41221 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41281 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41341 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41401 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41461 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41521 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41581 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41641 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41701 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41761 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41821 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41881 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    41941 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42001 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42061 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagcaaa
    42121 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42181 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42241 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42301 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42361 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42421 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42481 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42541 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42601 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42661 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42721 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42781 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgaaattgg gagacggtaa
    42841 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42901 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    42961 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt accggacaga
    43021 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43081 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43141 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43201 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43261 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43321 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43381 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43441 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43501 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43561 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43621 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43681 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43741 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43801 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43861 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43921 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    43981 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44041 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44101 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44161 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44221 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44281 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44341 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44401 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44461 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44521 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44581 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44641 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44701 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44761 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44821 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44881 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    44941 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45001 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45061 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45121 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45181 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45241 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45301 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45361 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45421 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45481 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45541 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45601 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45661 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45721 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45781 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45841 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45901 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    45961 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46021 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46081 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46141 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46201 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46261 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46321 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46381 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46441 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46501 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46561 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46621 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46681 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46741 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46801 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46861 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46921 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    46981 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47041 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47101 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47161 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47221 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47281 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47341 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47401 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47461 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47521 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47581 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47641 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47701 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47761 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47821 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47881 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    47941 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48001 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48061 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48121 gttctcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48181 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48241 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48301 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48361 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48421 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48481 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48541 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48601 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48661 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48721 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48781 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48841 ttgaaagaaa agtggcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48901 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    48961 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49021 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49081 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49141 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49201 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49261 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49321 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49381 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49441 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49501 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49561 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49621 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49681 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49741 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49801 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49861 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49921 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    49981 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50041 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50101 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50161 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50221 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50281 ttcagatagt caagatactg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50341 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50401 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50461 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50521 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50581 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50641 cttcgccctt cgcacgatgc agttggcgca aatgtatggc tatgtcattc cagagacact
    50701 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50761 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50821 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50881 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    50941 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51001 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51061 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51121 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51181 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51241 aagacaggtg caagaatcga gaaaaaggcg aatggcaagt aatgcaacgc gctaactttc
    51301 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51361 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51421 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51481 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51541 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51601 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51661 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51721 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51781 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51841 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51901 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    51961 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52021 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52081 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52141 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52201 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52261 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52321 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52381 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52441 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52501 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52561 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52621 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tattttttta tttttcttct
    52681 aaactttttt ctacctcaat cccccaagaa tctttcttga agtgattgat gatcaatgga
    52741 ataacgaaca tgaccactgt gatagctgct actaataatg catcactcaa tgtcgctgta
    52801 cctaatgtag caaatacgac aacgatgata gagaagatca gcatgcagct gactacccaa
    52861 gctaatccgt ttccgttttt accaattcgg taaggacgtt cgaattctgg atgtttctta
    52921 cgcattttaa gcaatgaaac agcaatcaaa atgtacacga tacaataaat aacagttgta
    52981 gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca ttacaccata aagcaaagca
    53041 aataatgaaa taacgattga ttgtgttaga acgacatttc tagatacgcc gtatttgttt
    53101 tctttatgga atccgaattt tggtggaagg aatccttcac gagctacttg gatgattgtt
    53161 tttgatggtc cagtaaccca agctgataat tgtaaaagca caccaataaa taccatgaag
    53221 ctgaagatat ttccgatcac ttctggtaag cctaggattt gacagtcaat caagataggc
    53281 tgcgtgatat ttgctaattc catttttcca tctggtacga catttgctac aagcatcgca
    53341 ttgatgacat tcaataaaac taatccgatc aaagcgataa atacgccttt tgtataattt
    53401 tttgttgcgt ctttcaaacg aggcatgtaa acagaactca tttcgatccc aacaaaaata
    53461 aatgtgatac cagctaagta tttcaatgaa tcaatatgtt ccaagtcagg gaaagctttt
    53521 gaccatgaaa agtcacctag atagccgttt gagactagtc cgactttgaa ggctgccaaa
    53581 acacctaaaa cgaacatgat gactactggg atatataccc ctagccatac accgatgttc
    53641 ccgccgactt ttgccatatc gaatttcaag ttcaagatcg tgatgatcca gtagataact
    53701 aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt ctacattacc aaatgtgttt
    53761 cctaacaatg gtcctaaagt agaagcgacc attaccatac ctgggaacat ttgcacccat
    53821 aatagccatg caacgacaaa tccccatttg ctgcccagtg ctgtttttac ccataactga
    53881 ggaccgcctt cttgaggaag catcgtggaa agctcaccgg aaaccattga tatcggaccg
    53941 gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg tccaaccgac tgcggctaac
    54001 gtaggaatac tccgaaccgt tgcacacagt gccattgtca gaccgataaa ggtcccaaga
    54061 cccattttac ttacttcaga tttactcacc gttgacactc tccttgattt tttggataaa
    54121 ataacggtgt ctttgaagta gtttcagcaa taaacgaaac aattcagcga atatttgagg
    54181 ggattctttg agtttattgc ttgaagggtt tcttcaaaga caacctcaaa acaaagggat
    54241 cgaagagctt aggtctcccc ttgctcttca gatccaaaca tgcgtcagaa acagttagta
    54301 ttcaagctgt tttctaacat gattaccttg cttattttgc ttcgcttgcc aataattggt
    54361 ctgcgtagat tttttccaat ttttcttgaa gagctgcttt gattttttca gcatattctt
    54421 caaagtgttc ttctttgttc atgtatggtg tcattacgct ggcacgcaat acagttactt
    54481 ttccggcacg gttccattct tcatctgaga atcctagttg gttcacaaat tgtaatgggc
    54541 tgttgccata gtctgggata gcaaagtcag tatgtgaagt taagaactca ttaccgtaaa
    54601 tgcttccttt cacgtatgaa gaataatcat atacgtcatg gttcaattta ttcattgcaa
    54661 ctaagtcatc gttgcctttt tctttgaata catagtctac catattgaaa tctggataag
    54721 ttaatggatg aacttcgatt tctttgtctc ctactttaaa tgacaagtca ttcaagaagt
    54781 tgtagaaacg atgtgcacct tcgattgatg cacccattaa tttaccatat cctgtgacat
    54841 tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc tgttgcccct gcttttgaac
    54901 cttccaagat ataagcccca agtaatgctg ggatatctgc gcctttttca aatacatatg
    54961 tcgcaaagta agagataacg tctctcatgc ggatatcttt gatgacgata ccaccggcag
    55021 aatatggaac atagcccatt ttatgtgggt caatcgttac agattctgct tcttcgattg
    55081 ctttatatgc actgtgtact tcttctaaga tataatcttt gttttctgta aagacattgt
    55141 atttgtaatg aacatctttt aaatcttcaa atggaatgaa gttgttgtct tcgtctaaga
    55201 aaatcgcacg gccgtatccg ccataagcag catctacgtg taagtagaag taaataccgt
    55261 ctttttctaa tacgcgacgc aatgcaacga ttttatcgat accgtcgatt gctccttctt
    55321 ctgttgatcc tacaacaccg actacaccta ggattggtgt tttttcagct gctagaccac
    55381 gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt gtggtcaact ggcactggaa
    55441 tcacttggtc taaaccgata ccgatgatgt cggcagcttt tagccatgaa tagtgtttag
    55501 tttgtggaac caaccatttt cctaattttt ctaaatgttt tccgctacgt gctgagtgag
    55561 ctttgatttc gtcgattttt tctgggacgc tgtctaacag attcatgatt tcttctgttg
    55621 acaagttcat taattcccaa tcgctttttc cagcaactag ttcaggtgtc acttctttca
    55681 ttgcaagcgg caatgatttg atgttgcggg cataccaaag tccttctaag tttgctaaag
    55741 aaccatcagc aacgatgtgg ccccagccat ctttatagct cattaatttt gcaaattcca
    55801 ttcctacttc ttcttccatt tggcttgttg caggagaaga ttcataggca acgttattac
    55861 cattccatag catagcaaaa ttgtacgcta gtaaagatgg catcaatgtt tcagagttca
    55921 tatgacccca gtaacgtccg gcattatgcc aaggaacgga atgggtacgc atccgcgctg
    55981 aaatttctga tagaacatcc tttgttttat tcacagtgtg ttcaaaacta gcactgcttt
    56041 tttcttctgg cgtaataata ggcatgtctt gaggcatgta gttttgacgc cagcctaaat
    56101 gttcatccac caattcattc aacaaagctt tatagatttg gccgttctca gctttgtccc
    56161 caatgaatag ggcatttagg tttagatctt tcgacaatga ttcactcata gtgaaaccct
    56221 cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca cctgttcttc tattctgtaa
    56281 actaagtttt ggcctgattt ttaatcatat tttaatatgt atcaattatc cgaattgttc
    56341 acacttttga aataattaaa aaaaacgagg gaatttacaa atgtaaactc cctcgcaaag
    56401 attgtattaa cgacaaccag cgttctattt taggatgggt ttcaaaattg gaatgttgta
    56461 atagtatttc cctttttcaa ggtaaaaact tactaaattc taatcaatta ctttggccaa
    56521 aaagtaattt ttcttacctt ttcgaataac cacaaactta ccatcgaatt catcggatgg
    56581 atttatgaca aaatttaaat cggtaacgcg gtcaccgtta atacttatag ctccgtttga
    56641 aacatcttca cgtgcttgac gtttagatgg ttcaattttt gtggaaacaa gcagttccac
    56701 gatattttca ggtgtgcttg aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat
    56761 ttcttccgca tttagttctt taatatttcc agagaacaat gcttgcgtta ttttttgcgc
    56821 ttctttcaag tcttcttcac tatgcacaaa tcttgtaact tcttctgcca aacggcgttg
    56881 tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct aagtcttcga tttcttcttg
    56941 agacaagaaa gtaaagaatt tcaagtattt cactacatcg cgatcatctt gattcaacca
    57001 gaattggtag aactcaaatg gtgaagtttt cttcggatca agccaaacgg caccgcctgc
    57061 agtttttccg aatttcgttc cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc
    57121 ttctgcttct ggtccttcta attttcggat taggtctaat ccagctgtga tatttcccca
    57181 ttggtctgct ccaccgattt gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc
    57241 gatagattgc aggatttggt aagtaaactc tgtaaatgag attcctactt ccaaacggct
    57301 ggcaacgata tcttttgcta acatcgtgtt gatgttgaag tttttaccgt agtctctcaa
    57361 aaattcaagt aaagagattt ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc
    57421 atctttacca aacaattttc tcatttgatt ggaaagtgcg tccacgttat gttgcacttg
    57481 ttccatcgtt tgcaacacac gttctgtttt gcggccgctt gggtcgccga tcgttcctgt
    57541 tccgccgcca atcagaatat atggatgatg gcctgccaat tggaatcttt tcatcatcat
    57601 aaatggaatc aaatgtccga tatgcatcga atcaccagtt ggatcaacgc cgcaataaag
    57661 tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct tcgtttgttt gctgattaat
    57721 ggcgtcgcgc caagttaatt catcaataat attcatttta ctgacctccg tgtagttttt
    57781 ttatgaaagc aggaacctgc tagcatatgt aattaattat ttcttcagga taggatagga
    57841 aataaaaaaa gtcccttggc atataagcca agggacgaaa tctcgcggta ccacccaatt
    57901 tgtatgtcta gatatccaac atacctctcg aacatttaac gctgtcgcgt ggcaatcact
    57961 gccaaagact cctgaaatgt aattcgcttt aaagcatgta ctggtttgca gcgaccacca
    58021 gctttctgaa acattggctt taacaactac ttcgtttcga tcttcgtctt gttattatta
    58081 atatgtgtct atcatagagc gcaacttttt ctttgtcaac agaaaaagga tatttttttt
    58141 aaattcctca ttttttcttt tttgaatccg ctctaattcc gaacatttta ttttcaatta
    58201 ttgttaaaag aaataacttt ttctgtattt tttctgcaat ttatcgagaa gtcactttac
    58261 tttgcctctt attctttctt cgttacaata aggaatggga caagtaaaaa aatgaaaaag
    58321 caagggacga ggtatatgga aaaaaagcaa aaaattattt taggagccac ttctgcttta
    58381 actatcgtta ttttaattct aagtttgttt tttgtcatcc gatcatctac aaaaaaagaa
    58441 ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag aaccgacaga tgataagcaa
    58501 caactagtta ctcaagcaaa aaatctggct caaagctacg attatgacca agcgattgcg
    58561 ttattggaaa aagatagtaa gaacggatct actgaaacac aacagcttct tcaaacacta
    58621 aaaaaggaga aagaacaact gatcgactgg tctgatccta ctcagatctc tcatgtcttt
    58681 ttccacagct tgatcgtgga cccgcaaaaa gccttccact ctcaacaggc acaaggatac
    58741 aaagattata tggtgactgt tgaagaattc aaccgttcga tcgaacaact ttatcaaaat
    58801 gattttgttt tagttaattt gaatgatttg atccaaaaag acgaacaagg aaatctttcc
    58861 tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc
    58921 agctattatg aatatatgga taattccggt tttcctagcc gattggtcgt atcaaataac
    58981 aatgaaatca aaaatgtcta taaagagaaa gataaagaag aaatcggtga ctttgacatg
    59041 gttcctctga tcgatgcctt tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa
    59101 ggtaccttgg ccctgacagg ttacaacgga gttttaggtt atcgaacatc aaaatctcaa
    59161 tatggagaca atgaaaaaac aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag
    59221 ttgaagaaag acggctggag ctttgcttcc catacatggg gtcacttgaa tatgactcaa
    59281 gcttcactag aagatgtaaa aaaagacaat gaattgtggc agcaagaagt tgcacctatc
    59341 ctaggaaaaa caaatattct gatttatcca tttggtgcag acatcagcga ctggcagcct
    59401 tattcagcag aaaatcaaaa atttgcttac ttgaagagtc aaggtttcga tattttctgt
    59461 aatgtcgatg cttctacacc agcttgggga caattaggta cggatttcta tcgaaatgct
    59521 cggatcaata tcgatggcat tcgttttcaa tctgatttag acggaaaaaa tcctattctt
    59581 gatccattca tcaatgtcca tgatgtctat gacaaagaag cacgcgaatc tgcttgattt
    59641 tacgtcattt atgaaccaaa aaagccgaac tgacatttta tcgtcagttt cggctttttt
    59701 atgctttatt tcttcttcgg tgcgattccc ttgatcagga cttctgtcca ttctgctgta
    59761 tatggaagca gttttgcgta tatctccggc ttagaaattc cttctaccat tgcttgaaaa
    59821 tagaggatca agaactcatc cgaatatttc aaatccactt ttccttcttt ccgccctcga
    59881 tggaaaagat caagcatgat actgaaactt tcttgcgtat acgcatccat cgcgtcagaa
    59941 aattcttttt tttctttttt tgtatagtat gtcatcaaat cgatataaaa ttgtttgttc
    60001 actcgattga gataacggat tttgttttca ctcatggcaa tcagtgtttc ttcaaatgga
    60061 agattttccg caatcaacgc tttcgcttct tgccctaaat cattcataaa tgcaaaaaat
    60121 acttcataaa tcagcccttc cttgctgcca aaatatttaa aaatcgtcgc tttacttacc
    60181 tgagcgttct cagctacctt atccattgtc actccagcgg ttcctatgtc tgtgttcatc
    60241 aagtcaaaag ctgcttgtaa aatctctttc tttttctctt gtgctctctt ttcaaaaccg
    60301 ttcatttgta gagcctcccg taattttaaa cttttataat atatatagtt caaatttatt
    60361 ctgaggttta ttataactat ctattgagaa agtatcaagc tagcaattcc ttg
//
LOCUS       DAYYCQ010000005        56963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000005 DAYYCQ010000000
VERSION     DAYYCQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.005"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(577..1080)
                     /locus_tag="ACHAYF_000350"
     CDS             complement(577..1080)
                     /locus_tag="ACHAYF_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8577817.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1439..2464
                     /locus_tag="ACHAYF_000351"
     CDS             1439..2464
                     /locus_tag="ACHAYF_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8577818.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2787..3665
                     /locus_tag="ACHAYF_000352"
                     /pseudo
     CDS             2787..3665
                     /locus_tag="ACHAYF_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            4141..4257
                     /locus_tag="ACHAYF_000353"
     CDS             4141..4257
                     /locus_tag="ACHAYF_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8577819.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5185..5523
                     /locus_tag="ACHAYF_000354"
     CDS             5185..5523
                     /locus_tag="ACHAYF_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577820.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5617..5847
                     /locus_tag="ACHAYF_000355"
     CDS             5617..5847
                     /locus_tag="ACHAYF_000355"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8577821.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6247..6618
                     /locus_tag="ACHAYF_000356"
     CDS             6247..6618
                     /locus_tag="ACHAYF_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8577822.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6892..7584)
                     /locus_tag="ACHAYF_000357"
     CDS             complement(6892..7584)
                     /locus_tag="ACHAYF_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8577823.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7587..8372)
                     /locus_tag="ACHAYF_000358"
     CDS             complement(7587..8372)
                     /locus_tag="ACHAYF_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8577824.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8609..10009
                     /gene="lacG"
                     /locus_tag="ACHAYF_000359"
     CDS             8609..10009
                     /gene="lacG"
                     /locus_tag="ACHAYF_000359"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8577825.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10034..10351
                     /locus_tag="ACHAYF_000360"
     CDS             10034..10351
                     /locus_tag="ACHAYF_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8577826.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10364..12037
                     /locus_tag="ACHAYF_000361"
     CDS             10364..12037
                     /locus_tag="ACHAYF_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8577827.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12051..13682
                     /locus_tag="ACHAYF_000362"
     CDS             12051..13682
                     /locus_tag="ACHAYF_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8577828.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14173..15738)
                     /gene="guaA"
                     /locus_tag="ACHAYF_000363"
     CDS             complement(14173..15738)
                     /gene="guaA"
                     /locus_tag="ACHAYF_000363"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8577829.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15797..16720)
                     /gene="coaA"
                     /locus_tag="ACHAYF_000364"
     CDS             complement(15797..16720)
                     /gene="coaA"
                     /locus_tag="ACHAYF_000364"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8577830.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16916..17521
                     /locus_tag="ACHAYF_000365"
     CDS             16916..17521
                     /locus_tag="ACHAYF_000365"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8577831.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17829..18845
                     /locus_tag="ACHAYF_000366"
     CDS             17829..18845
                     /locus_tag="ACHAYF_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8577832.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18986..20287
                     /locus_tag="ACHAYF_000367"
     CDS             18986..20287
                     /locus_tag="ACHAYF_000367"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8577833.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20333..20995
                     /gene="deoC"
                     /locus_tag="ACHAYF_000368"
     CDS             20333..20995
                     /gene="deoC"
                     /locus_tag="ACHAYF_000368"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8577834.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            21016..21414
                     /locus_tag="ACHAYF_000369"
     CDS             21016..21414
                     /locus_tag="ACHAYF_000369"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8577835.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21480..22562
                     /locus_tag="ACHAYF_000370"
     CDS             21480..22562
                     /locus_tag="ACHAYF_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8577836.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22774..24336
                     /locus_tag="ACHAYF_000371"
     CDS             22774..24336
                     /locus_tag="ACHAYF_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8577837.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24329..25462
                     /locus_tag="ACHAYF_000372"
     CDS             24329..25462
                     /locus_tag="ACHAYF_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8577838.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25462..26418
                     /locus_tag="ACHAYF_000373"
     CDS             25462..26418
                     /locus_tag="ACHAYF_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8577839.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26517..27683
                     /gene="deoB"
                     /locus_tag="ACHAYF_000374"
     CDS             26517..27683
                     /gene="deoB"
                     /locus_tag="ACHAYF_000374"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8577840.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27707..28525
                     /locus_tag="ACHAYF_000375"
     CDS             27707..28525
                     /locus_tag="ACHAYF_000375"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8577841.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28542..29249
                     /gene="deoD"
                     /locus_tag="ACHAYF_000376"
     CDS             28542..29249
                     /gene="deoD"
                     /locus_tag="ACHAYF_000376"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8577842.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29547..30233)
                     /gene="gpmA"
                     /locus_tag="ACHAYF_000377"
     CDS             complement(29547..30233)
                     /gene="gpmA"
                     /locus_tag="ACHAYF_000377"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8577843.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30405..30986)
                     /locus_tag="ACHAYF_000378"
     CDS             complement(30405..30986)
                     /locus_tag="ACHAYF_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8577844.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31119..31802)
                     /gene="rpiA"
                     /locus_tag="ACHAYF_000379"
     CDS             complement(31119..31802)
                     /gene="rpiA"
                     /locus_tag="ACHAYF_000379"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8577845.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32121..32534
                     /gene="rpsL"
                     /locus_tag="ACHAYF_000380"
     CDS             32121..32534
                     /gene="rpsL"
                     /locus_tag="ACHAYF_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8577846.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32618..33088
                     /gene="rpsG"
                     /locus_tag="ACHAYF_000381"
     CDS             32618..33088
                     /gene="rpsG"
                     /locus_tag="ACHAYF_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8577847.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33182..35269
                     /gene="fusA"
                     /locus_tag="ACHAYF_000382"
     CDS             33182..35269
                     /gene="fusA"
                     /locus_tag="ACHAYF_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8577848.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35441..36628
                     /gene="tuf"
                     /locus_tag="ACHAYF_000383"
     CDS             35441..36628
                     /gene="tuf"
                     /locus_tag="ACHAYF_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8577849.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            37035..37343
                     /gene="rpsJ"
                     /locus_tag="ACHAYF_000384"
     CDS             37035..37343
                     /gene="rpsJ"
                     /locus_tag="ACHAYF_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8577850.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37374..38003
                     /gene="rplC"
                     /locus_tag="ACHAYF_000385"
     CDS             37374..38003
                     /gene="rplC"
                     /locus_tag="ACHAYF_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8577851.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            38031..38654
                     /gene="rplD"
                     /locus_tag="ACHAYF_000386"
     CDS             38031..38654
                     /gene="rplD"
                     /locus_tag="ACHAYF_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8577852.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38654..38944
                     /gene="rplW"
                     /locus_tag="ACHAYF_000387"
     CDS             38654..38944
                     /gene="rplW"
                     /locus_tag="ACHAYF_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8577853.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38983..39816
                     /gene="rplB"
                     /locus_tag="ACHAYF_000388"
     CDS             38983..39816
                     /gene="rplB"
                     /locus_tag="ACHAYF_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8577854.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39859..40137
                     /gene="rpsS"
                     /locus_tag="ACHAYF_000389"
     CDS             39859..40137
                     /gene="rpsS"
                     /locus_tag="ACHAYF_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8577855.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40160..40507
                     /gene="rplV"
                     /locus_tag="ACHAYF_000390"
     CDS             40160..40507
                     /gene="rplV"
                     /locus_tag="ACHAYF_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8577856.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40521..41177
                     /gene="rpsC"
                     /locus_tag="ACHAYF_000391"
     CDS             40521..41177
                     /gene="rpsC"
                     /locus_tag="ACHAYF_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8577857.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41180..41614
                     /gene="rplP"
                     /locus_tag="ACHAYF_000392"
     CDS             41180..41614
                     /gene="rplP"
                     /locus_tag="ACHAYF_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8577858.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41604..41792
                     /gene="rpmC"
                     /locus_tag="ACHAYF_000393"
     CDS             41604..41792
                     /gene="rpmC"
                     /locus_tag="ACHAYF_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8577859.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41817..42083
                     /gene="rpsQ"
                     /locus_tag="ACHAYF_000394"
     CDS             41817..42083
                     /gene="rpsQ"
                     /locus_tag="ACHAYF_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8577860.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42141..42509
                     /gene="rplN"
                     /locus_tag="ACHAYF_000395"
     CDS             42141..42509
                     /gene="rplN"
                     /locus_tag="ACHAYF_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8577861.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42546..42854
                     /gene="rplX"
                     /locus_tag="ACHAYF_000396"
     CDS             42546..42854
                     /gene="rplX"
                     /locus_tag="ACHAYF_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8577862.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42882..43421
                     /gene="rplE"
                     /locus_tag="ACHAYF_000397"
     CDS             42882..43421
                     /gene="rplE"
                     /locus_tag="ACHAYF_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8577863.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43440..43625
                     /locus_tag="ACHAYF_000398"
     CDS             43440..43625
                     /locus_tag="ACHAYF_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8577864.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43661..44059
                     /gene="rpsH"
                     /locus_tag="ACHAYF_000399"
     CDS             43661..44059
                     /gene="rpsH"
                     /locus_tag="ACHAYF_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8577865.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            44091..44627
                     /gene="rplF"
                     /locus_tag="ACHAYF_000400"
     CDS             44091..44627
                     /gene="rplF"
                     /locus_tag="ACHAYF_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8577866.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44788..45144
                     /gene="rplR"
                     /locus_tag="ACHAYF_000401"
     CDS             44788..45144
                     /gene="rplR"
                     /locus_tag="ACHAYF_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8577867.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45165..45665
                     /gene="rpsE"
                     /locus_tag="ACHAYF_000402"
     CDS             45165..45665
                     /gene="rpsE"
                     /locus_tag="ACHAYF_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8577868.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45680..45859
                     /gene="rpmD"
                     /locus_tag="ACHAYF_000403"
     CDS             45680..45859
                     /gene="rpmD"
                     /locus_tag="ACHAYF_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8577869.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45903..46343
                     /gene="rplO"
                     /locus_tag="ACHAYF_000404"
     CDS             45903..46343
                     /gene="rplO"
                     /locus_tag="ACHAYF_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8577870.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46343..47638
                     /gene="secY"
                     /locus_tag="ACHAYF_000405"
     CDS             46343..47638
                     /gene="secY"
                     /locus_tag="ACHAYF_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8577871.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47700..48347
                     /locus_tag="ACHAYF_000406"
     CDS             47700..48347
                     /locus_tag="ACHAYF_000406"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8577872.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48538..48756
                     /gene="infA"
                     /locus_tag="ACHAYF_000407"
     CDS             48538..48756
                     /gene="infA"
                     /locus_tag="ACHAYF_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8577873.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48789..48905
                     /gene="rpmJ"
                     /locus_tag="ACHAYF_000408"
     CDS             48789..48905
                     /gene="rpmJ"
                     /locus_tag="ACHAYF_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8577874.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48923..49288
                     /gene="rpsM"
                     /locus_tag="ACHAYF_000409"
     CDS             48923..49288
                     /gene="rpsM"
                     /locus_tag="ACHAYF_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8577875.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49316..49705
                     /gene="rpsK"
                     /locus_tag="ACHAYF_000410"
     CDS             49316..49705
                     /gene="rpsK"
                     /locus_tag="ACHAYF_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8577876.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49785..50723
                     /locus_tag="ACHAYF_000411"
     CDS             49785..50723
                     /locus_tag="ACHAYF_000411"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8577877.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50751..51131
                     /gene="rplQ"
                     /locus_tag="ACHAYF_000412"
     CDS             50751..51131
                     /gene="rplQ"
                     /locus_tag="ACHAYF_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8577878.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51403..52242
                     /locus_tag="ACHAYF_000413"
     CDS             51403..52242
                     /locus_tag="ACHAYF_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8577879.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52218..53087
                     /locus_tag="ACHAYF_000414"
     CDS             52218..53087
                     /locus_tag="ACHAYF_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8577880.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            53077..53874
                     /locus_tag="ACHAYF_000415"
     CDS             53077..53874
                     /locus_tag="ACHAYF_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8577881.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            54077..54823
                     /gene="truA"
                     /locus_tag="ACHAYF_000416"
     CDS             54077..54823
                     /gene="truA"
                     /locus_tag="ACHAYF_000416"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8577882.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55487..55681
                     /locus_tag="ACHAYF_000417"
                     /pseudo
     CDS             55487..55681
                     /locus_tag="ACHAYF_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55869..56645)
                     /locus_tag="ACHAYF_000418"
     CDS             complement(55869..56645)
                     /locus_tag="ACHAYF_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8577883.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            56768..>56963
                     /locus_tag="ACHAYF_000419"
     CDS             56768..>56963
                     /locus_tag="ACHAYF_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFM8577884.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIH"
BASE COUNT        18205 a        10168 c        12157 g        16433 t
ORIGIN      
        1 atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa aaaataacat
       61 atctcatttt acacttttgt tattttatac ttaaaaaata acgacacaga aaataccgtg
      121 tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa tgccttcaaa
      181 aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta aataaggaga
      241 aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag aaaaataata
      301 tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata ttgactagga
      361 agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta aataagggga
      421 atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag tagattagag
      481 atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag atacacattg
      541 gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga cagttcgggt
      601 caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa taatggctag
      661 acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc ataaacctat
      721 cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa taccgaatag
      781 gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt ttttagaaaa
      841 agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga caatcaagtt
      901 ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa aagggacgag
      961 attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata gcttgaataa
     1021 aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata cataccgcat
     1081 agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc ataaaaaagt
     1141 atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt tttgttggga
     1201 atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc tatctttttt
     1261 ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt gtttactttt
     1321 tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg ctgacaaacg
     1381 ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga gggatagatt
     1441 gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta tcatgaatct
     1501 gatgctactt atctttattg tattatggtt aggttacata aatcatgatt ttttgatttc
     1561 aagtttttca tcattaggga tctttacttt tttctattat caaaacatac cgatgaaata
     1621 tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg ttttggggat
     1681 cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg ctttttccag
     1741 cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt ttgcgatgct
     1801 ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg tctcacttta
     1861 tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat tactggattc
     1921 acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg aagagccttt
     1981 agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt atgtcagtca
     2041 cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga ttttgctagc
     2101 agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat cgatgggtgg
     2161 gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga ccatttttct
     2221 gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg cagttgctaa
     2281 tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag catacttgat
     2341 ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg gtttaggtgt
     2401 tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc aaaaattaga
     2461 atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc acaacctcta
     2521 attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca aaaatgtgga
     2581 aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt atgatggtaa
     2641 ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag atattctttc
     2701 ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta taccatatgg
     2761 tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg ggaaatttga
     2821 tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt cccaaagtcg
     2881 ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt gagcaatata
     2941 gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata gacaaagtct
     3001 atgaagagga accttttgca gcattagcta tttatctata tgcagcttct caaagttgga
     3061 aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca atgaatgctt
     3121 ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt gaccgtggtt
     3181 tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg gagtacgaaa
     3241 tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag tgcctttctc
     3301 ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt tacgaaagtg
     3361 ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg tacacagata
     3421 cttggttact atcacccaat cttactaaat agttgaaaaa agagtcaaaa atctgtcttt
     3481 ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga gtcccttgga
     3541 tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt ttacagcgac
     3601 aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt attcttactt
     3661 cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa agaacacttt
     3721 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag
     3781 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt
     3841 aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta cgttcaatag
     3901 gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt ttttaggtag
     3961 ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct agtctgatcg
     4021 actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac acaaatcgta
     4081 attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg ggtcgtccat
     4141 atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa tatcaaaaca
     4201 cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa aaggtaagaa
     4261 gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt caataatcgg
     4321 tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac atatgataaa
     4381 ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat atagttaact
     4441 acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt ttcaattccc
     4501 tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca cctttgttcg
     4561 tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag gttttgcgaa
     4621 atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa ccattatcgt
     4681 ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc catttctttt
     4741 cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta actttaaaaa
     4801 gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta gaataacatc
     4861 aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt ggaattttag
     4921 atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca agtatcttta
     4981 ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt tgtagtcagt
     5041 cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg attggaagat
     5101 aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt tattttccct
     5161 ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga acatgataac
     5221 ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc ggagactcca
     5281 ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta tatacagtac
     5341 gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt agagctaaca
     5401 ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag tgacgagact
     5461 gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat attgtggatg
     5521 tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta gatgtagcac
     5581 aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa gggaaaagaa
     5641 aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg gaaagaattc
     5701 aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga aggatcgata
     5761 gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt agaagagttt
     5821 ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt ttgacacata
     5881 ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa tgcagtataa
     5941 agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct acacacttct
     6001 atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta gcttattgat
     6061 tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa cactcagaga
     6121 acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc tggttgggaa
     6181 aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga gtatgccgat
     6241 aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca atcccagtca
     6301 ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc ttcagggatt
     6361 agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa aattgtacgc
     6421 atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt taatgatact
     6481 tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga aacgtttatt
     6541 tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg tggagaggaa
     6601 ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca aaaaagtact
     6661 cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt taatgttaat
     6721 tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa gatacactgc
     6781 tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc agttttttat
     6841 aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg tttaagaatt
     6901 aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga tggttccatc
     6961 tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat caattgcaat
     7021 aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat cttcgataac
     7081 aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa tatccggttg
     7141 cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa accagcgggc
     7201 taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg tggcaatcgt
     7261 taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt ttgtcaactg
     7321 aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt cttctgacaa
     7381 tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa tctccgtatt
     7441 cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt ttgtatgatc
     7501 atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca ttgttccgtt
     7561 gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc ggttgaatca
     7621 gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga gatctggcga
     7681 tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc atctcgattg
     7741 aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc gatctgctgt
     7801 gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc atttgaactg
     7861 ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg tccgacaaat
     7921 gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc atctgcttga
     7981 gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt gttcagtaaa
     8041 tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc tccctcgtga
     8101 atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag agtggctttt
     8161 tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc tccatggaca
     8221 cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt catatcagaa
     8281 acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt gaccatttct
     8341 acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt aaggataatc
     8401 tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt tagggtggtt
     8461 tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt atttgtgttt
     8521 gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa agagcgaaaa
     8581 ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt ttatttttgg
     8641 cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca aaggcgctgt
     8701 tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg caagtgattt
     8761 ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa atggtctaag
     8821 attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa atccagaagg
     8881 aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa cgccgtttgt
     8941 gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt ttttgaatcg
     9001 agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat tcaaagaagt
     9061 caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat atttgttagg
     9121 tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc ttcataatat
     9181 gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc cgggtgaaat
     9241 tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg aagataagca
     9301 tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta cctatttagg
     9361 ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa atcaggccag
     9421 ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc gtaatgacta
     9481 cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag aaaatgcaat
     9541 ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg gaatcggaga
     9601 acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt atccagaagg
     9661 tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca aaatctatat
     9721 cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta tggatcagcc
     9781 gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa cagccggggt
     9841 aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga caaatgggta
     9901 taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt acccaaaaga
     9961 atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat aagtgataat
    10021 ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga tcgttgcata
    10081 ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac aaggaaactt
    10141 tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg cacataattc
    10201 gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg gattcatttt
    10261 tatccatggg caggatcatt taatgaccac tttattgctg agagatctga ttcaagactt
    10321 tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg gaatcattaa
    10381 acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt atttaggagc
    10441 cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca ttttcatttt
    10501 gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct ccacatggct
    10561 atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag caaccactgc
    10621 tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa tcaacaccac
    10681 ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc cgattgaagg
    10741 tggattttca acggaatata tggggacaaa gggactactg gcttcctttg tcgctgcatt
    10801 tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta aaatgcctaa
    10861 agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct ctttttcagt
    10921 atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct catttgcaga
    10981 agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact taggaatcgc
    11041 tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac cgtcgatcgt
    11101 ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc aattgttcaa
    11161 ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg taggaactat
    11221 gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg ctaagtctaa
    11281 gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg tcaacgaacc
    11341 attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat ttttgttaac
    11401 acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca tgaacagctt
    11461 tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag gaacgggcat
    11521 gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc tgatctatct
    11581 accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa aagcagaggt
    11641 gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg tggcagcgag
    11701 tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg ctggcgcagg
    11761 gacaagtggg atgttagcta atgctttaaa aaaaggtgca tctgaatcga aagcaccgat
    11821 cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt ttgatatggt
    11881 ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca ccgatcgctt
    11941 aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga gagatcctga
    12001 aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata atgaaaatgg
    12061 gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg ggggctgttt
    12121 caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt tcgatcctgt
    12181 cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa gatgccttag
    12241 agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag gatccttata
    12301 atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg attgaccttg
    12361 caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac agtgattttt
    12421 gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc tttgaagaaa
    12481 tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag cagctggacg
    12541 tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc tggccttatg
    12601 gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt ttgaagtctg
    12661 gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc cacttagctg
    12721 atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt cgaggtgtgt
    12781 catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg aatgaacttc
    12841 aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta gttgaaacag
    12901 cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt gaagccgaag
    12961 cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg ctggatcttg
    13021 ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat tgggaacctc
    13081 tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat ctcagtgttc
    13141 aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct ggaaacgcct
    13201 tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt aaaaatgcaa
    13261 gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg tttcaacaag
    13321 gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac tacaaactaa
    13381 ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt ggcataagca
    13441 aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat actaatcagt
    13501 tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa acaagtgaat
    13561 ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa attggggttt
    13621 atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg gtgaaacaat
    13681 agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc gttagcgtag
    13741 aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac gatttccagt
    13801 catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt atgctaaaga
    13861 gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc agataacata
    13921 aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt gagcacttag
    13981 atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt tttatgataa
    14041 gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc ttatttttaa
    14101 tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc ccctccatat
    14161 tatgttggct ctttattccc actccactgt tgctggtggt ttagaagtga tgtcatacac
    14221 gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt gcaagacttc
    14281 ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag cacggatacc
    14341 gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga tccctggaag
    14401 aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt cttcacgcaa
    14461 gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc caagaacacg
    14521 aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag gcatgcctaa
    14581 ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt cgatcaattc
    14641 aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg tctgtgctgt
    14701 ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga aagagacgcc
    14761 ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat
    14821 gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga agcgatctct
    14881 tgcattgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca ttacttgttc
    14941 cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat
    15001 cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa gaccaagcaa
    15061 gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga taaagttatc
    15121 cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca gcaattcgat
    15181 tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat tacgtgttgt
    15241 gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa agccttcagg
    15301 aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg gtgtttcttt
    15361 aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt cgcggttttt
    15421 agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat agcaaatccc
    15481 taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat tctcatcata
    15541 tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt ttacttcttc
    15601 tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa tacgtcttgt
    15661 gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa cgttcgtcat
    15721 atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct caaaatagtc
    15781 aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata tgattttcag
    15841 ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt
    15901 tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca cgatctccga
    15961 tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt aacgcaccaa
    16021 aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag acagaaaaat
    16081 caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct
    16141 caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg atatcataca
    16201 catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca cggttcaaga
    16261 aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc atgattccac
    16321 gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc
    16381 ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta ctttttccaa
    16441 cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat ttatgaagaa
    16501 ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa atcagatgag
    16561 caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc aaacttttga
    16621 tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat tctcgccatt
    16681 cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc tgcgttctcc
    16741 catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa gagagattgc
    16801 cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt gacttgcgca
    16861 cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg aagacatgac
    16921 aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat gggcttttga
    16981 gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac gtgaaacagt
    17041 ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac ctgatgaagg
    17101 aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg catttgcgac
    17161 acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa
    17221 cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct atgaagcagt
    17281 tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt
    17341 tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta cactgacaat
    17401 tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg
    17461 taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata
    17521 actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg
    17581 tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa
    17641 gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt
    17701 atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt
    17761 tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag
    17821 ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa
    17881 ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag
    17941 tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca
    18001 atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca
    18061 aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca
    18121 attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag
    18181 tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg
    18241 gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg
    18301 gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa
    18361 atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac
    18421 gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg
    18481 ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac
    18541 ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg
    18601 cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat
    18661 taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata
    18721 ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc
    18781 cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg
    18841 gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt
    18901 atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc
    18961 gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga
    19021 tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa
    19081 gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa
    19141 tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag
    19201 ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac
    19261 attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg
    19321 cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat
    19381 cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg
    19441 tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc
    19501 gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg
    19561 agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga
    19621 agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac
    19681 agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga
    19741 agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg
    19801 ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc
    19861 aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat
    19921 gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc
    19981 acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa
    20041 tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat
    20101 tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga
    20161 gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg aattattata
    20221 taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac
    20281 agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact
    20341 aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca
    20401 aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt
    20461 tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt
    20521 tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa
    20581 cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca
    20641 acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa
    20701 agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc
    20761 aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa
    20821 ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc
    20881 cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat
    20941 cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt
    21001 tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg
    21061 ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa
    21121 tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt
    21181 gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta
    21241 gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg
    21301 gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag
    21361 gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg
    21421 ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa
    21481 tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag
    21541 gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa
    21601 caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag
    21661 cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct
    21721 tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga
    21781 atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac
    21841 aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag
    21901 ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag
    21961 cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa
    22021 tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa
    22081 aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt
    22141 caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta
    22201 caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt
    22261 gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa
    22321 agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg
    22381 cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag
    22441 aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg
    22501 aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact
    22561 aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac
    22621 aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa caactccctg
    22681 aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc
    22741 cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa
    22801 atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa
    22861 gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg
    22921 atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa
    22981 gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag
    23041 cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct
    23101 agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa
    23161 cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa
    23221 caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag
    23281 ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta
    23341 gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta
    23401 gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac
    23461 gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg
    23521 aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa
    23581 aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt
    23641 ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg
    23701 cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc
    23761 cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta
    23821 gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac
    23881 agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa
    23941 gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat
    24001 cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca
    24061 aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa
    24121 caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat
    24181 gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa
    24241 gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga
    24301 aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt
    24361 attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc
    24421 ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca
    24481 aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt
    24541 ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg
    24601 gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct
    24661 tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt
    24721 acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt
    24781 attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt
    24841 ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc
    24901 ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt
    24961 ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc
    25021 ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat
    25081 ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt
    25141 ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg
    25201 ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg
    25261 tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc
    25321 tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt
    25381 ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa
    25441 ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct
    25501 acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga
    25561 agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc
    25621 gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta
    25681 gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg
    25741 cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt
    25801 ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg
    25861 tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat
    25921 acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc
    25981 aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca
    26041 ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc
    26101 ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc
    26161 gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg
    26221 gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa
    26281 ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca
    26341 atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac
    26401 tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt
    26461 cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt
    26521 ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg
    26581 ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga
    26641 acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat
    26701 cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata
    26761 cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc
    26821 ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg
    26881 tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga
    26941 aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg
    27001 aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa
    27061 aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag
    27121 acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc
    27181 ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt
    27241 tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc
    27301 agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt
    27361 ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat
    27421 ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta
    27481 cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac
    27541 cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt
    27601 cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa
    27661 gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag
    27721 cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt
    27781 tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat
    27841 gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa
    27901 gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta
    27961 ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg
    28021 ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg
    28081 agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc
    28141 gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta
    28201 tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata
    28261 tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat
    28321 aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg
    28381 attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct
    28441 gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt
    28501 taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag
    28561 cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat
    28621 atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag
    28681 gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc
    28741 catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc
    28801 gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac
    28861 aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc
    28921 caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata
    28981 cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga
    29041 cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt
    29101 acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga
    29161 tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag
    29221 taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag
    29281 taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat
    29341 tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc gtttaactct
    29401 gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt
    29461 gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg
    29521 tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac tgttaagtca
    29581 tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat gatgtcttcg
    29641 tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg tgcagctact
    29701 aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa tggcaaagcg
    29761 cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc taacattgcg
    29821 taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg aggaagagta
    29881 tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt
    29941 ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga tttgatttgt
    30001 ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat tgcacgagtt
    30061 aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt gcgtccgcct
    30121 tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc agtaaattgg
    30181 ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg catgtttagc
    30241 tctcctttta agataggttt atatttctga aattttcagt tctgttttat tgtacaattt
    30301 tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca agtagagaat
    30361 ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat gcataattgc
    30421 tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga aacaccgcat
    30481 tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg gatttttttg
    30541 atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg agcttcatct
    30601 aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag gttttccatt
    30661 aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag actaatcaag
    30721 tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt agatttgcct
    30781 aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc gatcactgac
    30841 tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg gaaaatttct
    30901 tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc atttatggtt
    30961 gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt ttctaactgg
    31021 atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc gcaaattttc
    31081 cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg gattgcgtca
    31141 agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt taggaaaagc
    31201 ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc tggctgttcg
    31261 atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa gacttctccg
    31321 ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg aagtagctgt
    31381 tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt atcgactaat
    31441 ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat tttctcgaaa
    31501 agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc atcagcaccg
    31561 tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat tcctaatttt
    31621 tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa accttcttca
    31681 cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt cccgagacct
    31741 acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg ttttaaattc
    31801 atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat tttagcgtga
    31861 tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa aaaaaattgc
    31921 atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt tatctacagg
    31981 tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga tgcagacatt
    32041 gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag acgcaaatcg
    32101 tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat
    32161 caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc tttaagaaaa
    32221 ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga
    32281 cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg tcaaacttaa
    32341 ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac agtgttgtat
    32401 tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc gttcgcggtg
    32461 ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac ggaactaaaa
    32521 aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta tatagaagta
    32581 attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag gtcctgttgc
    32641 aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc gcttgatcaa
    32701 ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac attatctata atgcctttga
    32761 cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag caatgaaaaa
    32821 cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact accaagtacc
    32881 agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag taaactacgc
    32941 tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca tggatgctgc
    33001 aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa
    33061 ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct tttataagtc
    33121 gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag aaacaaggag
    33181 aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg cccacatcga
    33241 tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa tccataaaat
    33301 tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac aagaacgtgg
    33361 tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa tcaacatcat
    33421 cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc gtgtacttga
    33481 tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg aaacagtttg
    33541 gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat
    33601 cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag caaacgctca
    33661 cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg accttgtaac
    33721 aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa cagaaattcc
    33781 tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga
    33841 aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg aagctgagtt
    33901 aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac tatgtggttc
    33961 agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt acttgccatc
    34021 accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag aagtagaacg
    34081 tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga ctgacccatt
    34141 cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt
    34201 acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa
    34261 ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg ttggtttgaa
    34321 agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc ttgaatccat
    34381 ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga
    34441 taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc gtgctgaaac
    34501 aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact tagatatcat
    34561 cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc
    34621 ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac gtcagtctgg
    34681 tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag aaggtgctgg
    34741 tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt
    34801 tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc cattagtaga
    34861 tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg aaactgcctt
    34921 ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc cagtaatcct
    34981 tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg atgttatggg
    35041 tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg gcggccaaca
    35101 agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa ctcttcgttc
    35161 tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag atgtaccaaa
    35221 atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat ttcttgaaac
    35281 cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag tttcgtgtaa
    35341 aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata cctatcagac
    35401 aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag aaaaatttga
    35461 ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg gtaaaactac
    35521 attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag ctatggctta
    35581 cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct caacagctca
    35641 cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag gacacgcgga
    35701 ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct tggtagtttc
    35761 tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg
    35821 tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg aagaattact
    35881 agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc ctggtgacga
    35941 tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt catacgaaga
    36001 aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag aacgtgacaa
    36061 cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac gtggtactgt
    36121 tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg aagttgttgg
    36181 tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt
    36241 agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg cacgtgaaga
    36301 catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata caaaattctc
    36361 tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat tcttcactaa
    36421 ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg aattaccaga
    36481 aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat taatccaccc
    36541 aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta cagttggtgc
    36601 cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa gcaacattta
    36661 ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct tttttattta
    36721 ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga aataaaagag
    36781 tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg
    36841 ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg aattgcacag
    36901 acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc ccggacgctt
    36961 tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc aaaataggcg
    37021 aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc
    37081 gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact ggagctagcg
    37141 tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc cgtgcgactc
    37201 ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt cttatcgaca
    37261 ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta ccatctggtg
    37321 taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta ctcatgacca
    37381 aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct ggtgaattaa
    37441 ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa acaatggaaa
    37501 cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt ttatcaaaca
    37561 aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc attcgtgaat
    37621 tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta gatgttttcc
    37681 aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc caaggggtta
    37741 tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc
    37801 ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc
    37861 gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc gatgcagaaa
    37921 gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta attactatca
    37981 aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga atgccgaatg
    38041 tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac gaagaaatct
    38101 tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat
    38161 taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac
    38221 catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc
    38281 gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa cttcctaaaa
    38341 aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg
    38401 tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa caagttcttg
    38461 ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac gactttgctg
    38521 ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc
    38581 tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact caaattgagg
    38641 aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc
    38701 aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg caaacaaaac
    38761 tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt
    38821 gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa aaaaacgtcg
    38881 caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg aagctgctga
    38941 ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat taaaaagtac
    39001 aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga aatcacaaca
    39061 tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg taacaacaac
    39121 ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac
    39181 ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata tgatccaaac
    39241 cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta catcttagca
    39301 cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc
    39361 ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa cattgaaatg
    39421 aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca agtacttggt
    39481 aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat gatcttagct
    39541 gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat caacatcggt
    39601 aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac
    39661 cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg taaagcacca
    39721 gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa agctaaatca
    39781 gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg tgtcgcacat
    39841 tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat
    39901 ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg
    39961 tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt ttatgatgga
    40021 cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt aggtgaattt
    40081 gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa acgctaattt
    40141 gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac
    40201 agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag gtaaaagtgt
    40261 cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa tcattgaaaa
    40321 agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg ttgaaaactt
    40381 ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc
    40441 aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag tcgtatcaga
    40501 aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca
    40561 tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg
    40621 aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct gtatcaacag
    40681 ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct aaaccaggta
    40741 tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta aacaaattaa
    40801 ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat
    40861 tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa
    40921 aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact caagtatctg
    40981 gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga actgttcctc
    41041 ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca acatacggaa
    41101 aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa aaaaacactg
    41161 agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc gtgaattccg
    41221 cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg aatacggttt
    41281 gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat
    41341 gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc acaaatctta
    41401 tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg aaggatgggt
    41461 tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc
    41521 tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga tcgtaaaacg
    41581 tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta accactgccg
    41641 aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga ttccaattag
    41701 caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg attgcacgca
    41761 tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt aagcgtatga
    41821 ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac aaaatggata
    41881 aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc aaacgcatga
    41941 actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg
    42001 tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg ttggaagtag
    42061 tcgaagaagc agttattatc taataacacg agattttcct atatagattg aatgcaaaat
    42121 ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc gctgataact
    42181 caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt aaaactgcta
    42241 acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg gttgtcaaaa
    42301 aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt cgtacagacg
    42361 gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat aaaagccctc
    42421 gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga
    42481 tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg aggtgcgaaa
    42541 cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa gacaaaaaca
    42601 aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc gaaggtgtaa
    42661 atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga attgttgaga
    42721 tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt gtagctggcc
    42781 gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag
    42841 ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa
    42901 aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc tctgttatgc
    42961 aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg
    43021 ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa aaaccaatga
    43081 ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg ccaattggtg
    43141 caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg gtttctgttt
    43201 cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa
    43261 actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat gatttagtag
    43321 ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat gaagaatctc
    43381 gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag gcgaactacg
    43441 tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca acacaagctt
    43501 atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc catctttgcc
    43561 gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag aaagctagct
    43621 ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga cagatccaat
    43681 tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt ctttagaagt
    43741 acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg
    43801 tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg
    43861 taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc
    43921 ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg caatcatttc
    43981 gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt
    44041 tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa gtgagccgta
    44101 tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac ggaaacaacg
    44161 ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat atcaaaatga
    44221 atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa atgaaaacaa
    44281 ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc gaaggtttcc
    44341 aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga tcaaaacttg
    44401 tattgaacgt tggttactca catccagtag aaatcacacc accagctgga gtaactgttg
    44461 aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat
    44521 tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa ggtattcgtt
    44581 atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa
    44641 ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt caaaaaaata
    44701 catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt gaaatagtag
    44761 tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa caaaacacgt
    44821 caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg cccacgcttg
    44881 aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt agcgggtgta
    44941 acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa aacagaagca
    45001 gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat taaagtagta
    45061 gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc tgaagctgct
    45121 cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt tatatcgatc
    45181 caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta
    45241 aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa aacggacacg
    45301 tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag
    45361 atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca cacgaagtaa
    45421 tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt tctggggttg
    45481 ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat atcacttcta
    45541 aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac
    45601 aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg
    45661 gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca gtattatcgg
    45721 acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga ttaacagttc
    45781 agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt ctcacctagt
    45841 ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga ggtgccaaac
    45901 ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg
    45961 gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag
    46021 ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca ttgttccgtc
    46081 gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc attaatcttg
    46141 atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta gttgaagctg
    46201 gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa ctaaacaaga
    46261 aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt gaagcggctg
    46321 gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta aagtaaaaga
    46381 cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca
    46441 tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt tgccattttt
    46501 caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt tctcaatggg
    46561 ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg acatcgtgcc
    46621 tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga atcaagcgac
    46681 tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg ctggattcaa
    46741 tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg taacgatcgg
    46801 tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga tcacagaaaa
    46861 aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta gattgcctga
    46921 aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag acatatggaa
    46981 atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga cattcgtaac
    47041 atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg tagctggcgc
    47101 gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc cagttatctt
    47161 tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg gtagattcgg
    47221 ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag cacctggtgc
    47281 tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa
    47341 ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc
    47401 tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta cagttggtgc
    47461 agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt gggatcttcc
    47521 acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag ctttagaaac
    47581 tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca ttgagtaaga
    47641 aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga gggaaaacaa
    47701 tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct gaaaaaatca
    47761 ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca gcgatgcaaa
    47821 acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca ttagttcctg
    47881 atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca gagaaaggct
    47941 tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac gcaatgctaa
    48001 aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg
    48061 tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac cataaaattt
    48121 tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa
    48181 gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc aaaaatagtg
    48241 aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat ggtgctcgtg
    48301 aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac gattgcccgt
    48361 agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat agaatatgtc
    48421 agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag gtgggaactg
    48481 cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg
    48541 gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg
    48601 tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg taaaattcgt
    48661 atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc tccatacgat
    48721 cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa actacaggag
    48781 gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc
    48841 gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag
    48901 gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc ctcgtgataa
    48961 acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg ctaaaaagat
    49021 cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac
    49081 agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt
    49141 gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc gtcatcgccg
    49201 tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta aaggcccagc
    49261 tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa acttcatggc
    49321 agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag gtattgcaca
    49381 tatccattct acattcaata acactatcat catgatcact gatactcacg gaaatgcatt
    49441 agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa ctccttttgc
    49501 cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat taaagactgt
    49561 tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt cattacaagc
    49621 tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata atggtgcccg
    49681 ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta tcagtaacgt
    49741 cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt gagttcgaaa
    49801 aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc gttgaacctc
    49861 ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta ttatcttctt
    49921 tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa ttctctacca
    49981 tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga
    50041 aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct gctacagtaa
    50101 ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat atgtacatct
    50161 gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct ggacgtggtt
    50221 atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt ccagtcgatt
    50281 caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt gttggacacc
    50341 gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt gaaccgttgg
    50401 aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt gtgaacttaa
    50461 ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa
    50521 tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat tgtttgaaac
    50581 gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa atgatcaaag
    50641 tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac gacttaggtt
    50701 taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac gtgagttatc
    50761 gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta acaactgatt
    50821 tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt cgctcaactg
    50881 cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg
    50941 ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat
    51001 cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt caaggcggat
    51061 acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag
    51121 aacttgtcta aaatcacact aataacatca ctttatagat gattctttta gagtgttatg
    51181 atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga atatcgcttg
    51241 gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt catgacagat
    51301 atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt tgtctttttt
    51361 tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc aattattgaa
    51421 ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt gaaagatgtc
    51481 tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg ttcaggaaaa
    51541 tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat cgtgaaagtg
    51601 ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt tggaatggtt
    51661 tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt tgcttttggt
    51721 cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag
    51781 aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa
    51841 caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat tttagatgaa
    51901 gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat caagaagatc
    51961 aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga agcagctaat
    52021 gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa
    52081 attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt tcctgaaaaa
    52141 ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat gacagaagaa
    52201 aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact tatcagccga
    52261 atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa gaaaacagtt
    52321 atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag catttgaatg
    52381 cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt cagccggata
    52441 cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt cagtttccag
    52501 aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct aaaaactttg
    52561 gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa gttgggctgg
    52621 atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg cgtagagtag
    52681 caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa ccaacggcag
    52741 gacttgatcc ccagggtaga aaagaaatga tggagatgtt ttggcgtctt cataaggaac
    52801 agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat tttgcagatt
    52861 atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc
    52921 aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc gagtttgcag
    52981 aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct gaagaattag
    53041 cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt gatatttggt
    53101 cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa attattagcc
    53161 agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta ccttttgttg
    53221 gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt ttttatccga
    53281 ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt cttgtttaca
    53341 agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt tggtgtgatg
    53401 aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac tctactcacg
    53461 cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg tccattgaaa
    53521 gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct tcgttttgtg
    53581 cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg cggtgttgat
    53641 tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct gattccttta
    53701 tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc aagaggctac
    53761 aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag agacacgatg
    53821 gttatcattg cttttgtatt attaactgtg ctactgatct ttttgagagg ataaacgttc
    53881 agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat gcgagcagtg
    53941 tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt tcttttatct
    54001 gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat tccagataac cttttaggaa
    54061 caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcatacgacg gaacaaattt
    54121 taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag aaaaaacatt
    54181 acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga cagatgccgg
    54241 agtacatgcg attgggcaag taatccattt tgattatcca gaggagcgtc cgttagaacg
    54301 aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac aagtagaaat
    54361 tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc aatttcgagt
    54421 ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt ttccttaccc
    54481 tcttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc acgatttcac
    54541 ctctttttgt gcatcaggaa gctcgataga ggataaagta cgtacgatct acgaggctaa
    54601 gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa atggcttctt
    54661 gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg gccgattgcc
    54721 tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag gacctacagc
    54781 acatcctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggttg tgacagaagt
    54841 ggtcagcttc aagaaataag aaggaattta cgaaaattac ttctcaaaat tttgcaaaat
    54901 ttcggcttat tttcgaagga gctacttctg gaacaccgtg aatcagaggt tgtgaaaaag
    54961 ctgccctgca tcaagtaata agaacaacca aacaaaaata gctcgtcata tttttcgttt
    55021 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    55081 atcagcgttt tgacctgagt attagagaaa caaacggaaa aatagttcct catattttgc
    55141 cgtttgttga gctaataccg aaggtcagct gattgaacac cgtttaagaa agaggtcgtg
    55201 agcctgtcat ttaactctga gtcacaagta acaatttcaa aaaacagctt ctcatctttg
    55261 atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt
    55321 ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttaatga ggacagtcgg
    55381 aacaggtttg tcatttttat atttaaatgg cacaaatttt tctaattatt tgtcatttag
    55441 cgctgaaaat ttttgctatt tatctaaaaa agtgataact acttaagatg aagcagaata
    55501 tttacgccat acggcataca accgcgaaaa atataaaaaa aagaaaacga ttgaacgcgt
    55561 ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc aagtatcgag acttggaaaa
    55621 caatcaggtg tatacgctga ttgtttttgc tgcaatgaat ctgaaaaagt tgtccacttg
    55681 gcagttggaa catatgtctt catatctttt attatacaaa aatagcaaaa gcaccagtct
    55741 acactcttag taaagaggaa actgatcctt ttgtctacat tcttaaagta cttaaactaa
    55801 gtactttatt ttactcttta ggattgatac agtagcatct atccctttat taataatctt
    55861 ttattttttt aaataaagat gttgtctcca tcttcaggca ctaaaacttt ttcggaaatt
    55921 cctttttctt tattgaaaga ctttaattct tctcttgaca gtccccaatg gtttacagct
    55981 tccatatgaa cggcaataat tgtggaatct ggtattgctt tatgcaattc atataaatcg
    56041 tctttcccca taataagaga ttctccaact aaaaaatgat tatctccagc atttgctact
    56101 atgatttctg gattatactg atcaatagtt tgtttaacag cttcatacca aactgtatct
    56161 cctgtaaagt atagtgtctt ctcagtcgaa tgtttgaaaa caataccgca aacattacct
    56221 acataattca agatttctcc tctgccatgt ttgccttttg ttttaaccaa ttgtatagat
    56281 tcaaaaactg tgtcttctcg tagtacctcc acatttttaa aaccatatag tcgaatttca
    56341 ttcgcatcat tttcatcttg cacaaataat tttatttctt taggtaggac ctctttcgct
    56401 atatcgtccc aatgatctag atgtaaatgt gttacaataa cagcatcaat accgttgata
    56461 atatcttcga ctggcattgg caaatcaact agaggattca tttgatcttt tcttggtgat
    56521 ggaaaaggac ctttcgaaat aaatggtgga agtgttcctt tttccgctaa cattggatca
    56581 attaaaaatt ttttacctcc atagtctaag atagctgttg cattacgaat gtgatgtata
    56641 ttcataaacg ttgtctcctt tttgttgata tgaaatattt ttagtataat tcaattccat
    56701 acaataaata aaccgaacaa aaaaagtctt ttactatttt tacttaattt atgaaaggtt
    56761 gtttaccatg ttagaccaaa cagatataaa tatcattgag gaactatcaa aaaatagtcg
    56821 aatcacaatg aaagaacttg gagaaaaagt acatttgact ggacccgctg tctcggcgag
    56881 agtgacaaaa ctagaggaaa gtggagttat tgaaaattat acagtaaaat taaatatgga
    56941 aaagctaggg ttttcgattc acg
//
LOCUS       DAYYCQ010000006        55411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000006 DAYYCQ010000000
VERSION     DAYYCQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.006"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..996
                     /locus_tag="ACHAYF_000420"
     CDS             139..996
                     /locus_tag="ACHAYF_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8577885.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1111..2031
                     /gene="pstC"
                     /locus_tag="ACHAYF_000421"
     CDS             1111..2031
                     /gene="pstC"
                     /locus_tag="ACHAYF_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8577886.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2031..2915
                     /gene="pstA"
                     /locus_tag="ACHAYF_000422"
     CDS             2031..2915
                     /gene="pstA"
                     /locus_tag="ACHAYF_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8577887.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2930..3736
                     /gene="pstB"
                     /locus_tag="ACHAYF_000423"
     CDS             2930..3736
                     /gene="pstB"
                     /locus_tag="ACHAYF_000423"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8577888.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3752..4510
                     /gene="pstB"
                     /locus_tag="ACHAYF_000424"
     CDS             3752..4510
                     /gene="pstB"
                     /locus_tag="ACHAYF_000424"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8577889.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4523..5200
                     /gene="phoU"
                     /locus_tag="ACHAYF_000425"
     CDS             4523..5200
                     /gene="phoU"
                     /locus_tag="ACHAYF_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8577890.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5438..5737
                     /locus_tag="ACHAYF_000426"
     CDS             5438..5737
                     /locus_tag="ACHAYF_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577891.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5778..6470
                     /locus_tag="ACHAYF_000427"
     CDS             5778..6470
                     /locus_tag="ACHAYF_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8577892.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6467..7234
                     /locus_tag="ACHAYF_000428"
     CDS             6467..7234
                     /locus_tag="ACHAYF_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8577893.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7334..8902
                     /gene="liaX"
                     /locus_tag="ACHAYF_000429"
     CDS             7334..8902
                     /gene="liaX"
                     /locus_tag="ACHAYF_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8577894.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9199..9519
                     /locus_tag="ACHAYF_000430"
     CDS             9199..9519
                     /locus_tag="ACHAYF_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8577895.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9519..9869
                     /locus_tag="ACHAYF_000431"
     CDS             9519..9869
                     /locus_tag="ACHAYF_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8577896.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10036..10974
                     /gene="hprK"
                     /locus_tag="ACHAYF_000432"
     CDS             10036..10974
                     /gene="hprK"
                     /locus_tag="ACHAYF_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8577897.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10989..11819
                     /gene="lgt"
                     /locus_tag="ACHAYF_000433"
     CDS             10989..11819
                     /gene="lgt"
                     /locus_tag="ACHAYF_000433"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8577898.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11863..12888
                     /locus_tag="ACHAYF_000434"
     CDS             11863..12888
                     /locus_tag="ACHAYF_000434"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8577899.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12903..13841
                     /gene="galU"
                     /locus_tag="ACHAYF_000435"
     CDS             12903..13841
                     /gene="galU"
                     /locus_tag="ACHAYF_000435"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8577900.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13966..14406
                     /locus_tag="ACHAYF_000436"
     CDS             13966..14406
                     /locus_tag="ACHAYF_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8577901.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14409..15011
                     /locus_tag="ACHAYF_000437"
     CDS             14409..15011
                     /locus_tag="ACHAYF_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8577902.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15079..16185)
                     /locus_tag="ACHAYF_000438"
     CDS             complement(15079..16185)
                     /locus_tag="ACHAYF_000438"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8577903.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16395..17396
                     /gene="ccpA"
                     /locus_tag="ACHAYF_000439"
     CDS             16395..17396
                     /gene="ccpA"
                     /locus_tag="ACHAYF_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8577904.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17699..20044)
                     /locus_tag="ACHAYF_000440"
     CDS             complement(17699..20044)
                     /locus_tag="ACHAYF_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8577905.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20323..21225
                     /locus_tag="ACHAYF_000441"
     CDS             20323..21225
                     /locus_tag="ACHAYF_000441"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8577906.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21438..23213
                     /locus_tag="ACHAYF_000442"
     CDS             21438..23213
                     /locus_tag="ACHAYF_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8577907.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23470..24687
                     /locus_tag="ACHAYF_000443"
     CDS             23470..24687
                     /locus_tag="ACHAYF_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8577908.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(25134..26720)
                     /locus_tag="ACHAYF_000444"
     CDS             complement(25134..26720)
                     /locus_tag="ACHAYF_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8577909.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            26971..27195
                     /locus_tag="ACHAYF_000445"
     CDS             26971..27195
                     /locus_tag="ACHAYF_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8577910.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27337..29088
                     /locus_tag="ACHAYF_000446"
     CDS             27337..29088
                     /locus_tag="ACHAYF_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8577911.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            29088..30872
                     /locus_tag="ACHAYF_000447"
     CDS             29088..30872
                     /locus_tag="ACHAYF_000447"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8577912.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            31330..32181
                     /gene="licT"
                     /locus_tag="ACHAYF_000448"
     CDS             31330..32181
                     /gene="licT"
                     /locus_tag="ACHAYF_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8577913.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            32278..34164
                     /locus_tag="ACHAYF_000449"
     CDS             32278..34164
                     /locus_tag="ACHAYF_000449"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8577914.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34205..35107)
                     /locus_tag="ACHAYF_000450"
     CDS             complement(34205..35107)
                     /locus_tag="ACHAYF_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8577915.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            35337..36740
                     /locus_tag="ACHAYF_000451"
     CDS             35337..36740
                     /locus_tag="ACHAYF_000451"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8577916.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            36740..38173
                     /locus_tag="ACHAYF_000452"
     CDS             36740..38173
                     /locus_tag="ACHAYF_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8577917.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38318..39244
                     /gene="trxB"
                     /locus_tag="ACHAYF_000453"
     CDS             38318..39244
                     /gene="trxB"
                     /locus_tag="ACHAYF_000453"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8577918.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39457..41184
                     /locus_tag="ACHAYF_000454"
     CDS             39457..41184
                     /locus_tag="ACHAYF_000454"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8577919.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            41200..41550
                     /locus_tag="ACHAYF_000455"
     CDS             41200..41550
                     /locus_tag="ACHAYF_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8577920.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(41634..42494)
                     /locus_tag="ACHAYF_000456"
     CDS             complement(41634..42494)
                     /locus_tag="ACHAYF_000456"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8577921.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            42586..43014
                     /locus_tag="ACHAYF_000457"
     CDS             42586..43014
                     /locus_tag="ACHAYF_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8577922.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43176..43352
                     /gene="rpsU"
                     /locus_tag="ACHAYF_000458"
     CDS             43176..43352
                     /gene="rpsU"
                     /locus_tag="ACHAYF_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8577923.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43379..43825
                     /locus_tag="ACHAYF_000459"
     CDS             43379..43825
                     /locus_tag="ACHAYF_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8577924.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(43981..45360)
                     /gene="brnQ"
                     /locus_tag="ACHAYF_000460"
     CDS             complement(43981..45360)
                     /gene="brnQ"
                     /locus_tag="ACHAYF_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8577925.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            46066..47037
                     /locus_tag="ACHAYF_000461"
     CDS             46066..47037
                     /locus_tag="ACHAYF_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8577926.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            47061..49253
                     /locus_tag="ACHAYF_000462"
     CDS             47061..49253
                     /locus_tag="ACHAYF_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8577927.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            49270..49746
                     /gene="ybeY"
                     /locus_tag="ACHAYF_000463"
     CDS             49270..49746
                     /gene="ybeY"
                     /locus_tag="ACHAYF_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8577928.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            49730..50125
                     /locus_tag="ACHAYF_000464"
     CDS             49730..50125
                     /locus_tag="ACHAYF_000464"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8577929.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            50140..51039
                     /gene="era"
                     /locus_tag="ACHAYF_000465"
     CDS             50140..51039
                     /gene="era"
                     /locus_tag="ACHAYF_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8577930.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51182..51994
                     /gene="recO"
                     /locus_tag="ACHAYF_000466"
     CDS             51182..51994
                     /gene="recO"
                     /locus_tag="ACHAYF_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8577931.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            52378..53295
                     /gene="glyQ"
                     /locus_tag="ACHAYF_000467"
     CDS             52378..53295
                     /gene="glyQ"
                     /locus_tag="ACHAYF_000467"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8577932.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            53297..55372
                     /gene="glyS"
                     /locus_tag="ACHAYF_000468"
     CDS             53297..55372
                     /gene="glyS"
                     /locus_tag="ACHAYF_000468"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8577933.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17957 a         9933 c        11784 g        15737 t
ORIGIN      
        1 atagcataga aacttttaat gagatagttt tttttacaat tatgagaatt tattttgtta
       61 tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag
      121 gattaaagga gatttctaat gaaaaaactt aaattattct taccaataat aggattagcc
      181 attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc
      241 tcttctgctt tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct
      301 ggaaaattca tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca
      361 ggagcagtcg acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc
      421 tcagcattag tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat
      481 attggcgtaa aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa
      541 aactggagtg aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga
      601 agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca
      661 caagaacaag attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc
      721 agctatgttg ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta
      781 gaaccgacag atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg
      841 tacacaagag atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat
      901 gaagtacaag ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc
      961 gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt
     1021 agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt
     1081 atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca
     1141 aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg
     1201 ctgatcgtgt tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc
     1261 ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc
     1321 caaatcggag caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt
     1381 attgtgacct ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc
     1441 atgacggaga tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt
     1501 gtaggtattc cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc
     1561 agaagtattt ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt
     1621 atgatcttgc cgactgtcac aacgatgact gttgatgcat taaaggcagt gccacggcac
     1681 tatcgggaag cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg
     1741 ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt
     1801 ggtgaagcct tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt
     1861 actactcctg cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg
     1921 ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta
     1981 ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa
     2041 aacgctcaga taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga
     2101 ttttagcagc tttactactt tatatcttag ttcggggaat accgcatatt tcatggtcct
     2161 tcttgaccga accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta
     2221 actcgattta tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg
     2281 gtatctatct ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga
     2341 ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg
     2401 ttattcaatt ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca
     2461 acttgcctct tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac
     2521 gagaagcagg attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc
     2581 cagaagcgac accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg
     2641 aagcagctgc attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact
     2701 ggaatccatt aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg
     2761 tccatatttg gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg
     2821 gcgcttctgc cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg
     2881 gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata
     2941 taactggaat gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac
     3001 ggatgatcta catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt
     3061 tgaaaaaaat aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct
     3121 tcgttcgttg aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat
     3181 gtatcaaggc gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat
     3241 tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt
     3301 tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag
     3361 cttgaaacaa gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc
     3421 tttatctggc gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga
     3481 tatcttgctg ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga
     3541 agaaacactt gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca
     3601 acaagcagct cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata
     3661 tgacaaaact aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc
     3721 cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga
     3781 tcttcatctg tattatggaa agaaagaagc actaaaaggg atcaatctaa gtttcaatca
     3841 aggggaaatc actgcgatga tcggtccttc tggttgcggg aaatccactt atctgcgttc
     3901 tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa
     3961 agacaaagac atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat
     4021 ggtattccag cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt
     4081 gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa
     4141 ggcagcgtct gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc
     4201 cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat
     4261 cttattggat gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat
     4321 gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc
     4381 ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg aatacaacga
     4441 tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg
     4501 atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct
     4561 ccataatcag ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg
     4621 tgcatacatc aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat
     4681 caataacatg gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc
     4741 agttacaact gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg
     4801 tatggccgat catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag
     4861 aataccggaa atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga
     4921 caacgtccta gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat
     4981 ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca
     5041 ggaaaatcct gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga
     5101 acggatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa
     5161 aatcactgag ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc
     5221 gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag
     5281 taataagaac agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta
     5341 ccgcagatgc aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc
     5401 acaaaccctt ttcatcattt catagaaagg ataaagaatg caaaaaaaaa ttagcatcat
     5461 cactatattt tttatttgtg tgcttacttc atgggttatt cagcatcaag ctgctttata
     5521 tgaagtacct gtcatcgaga tcgaaaaagt acgaataaaa gaggatcatc aattgattac
     5581 aggaagactg atgaacacgg atcaaaaagg agaatcgatt tatttatcgg ttccttatca
     5641 aaaaaatcaa atagaaacag caaatataaa agcgagacaa atgtatctag tccaaatgca
     5701 tggagatgaa tggcagctag tagcaaaaaa aaaatgatgg ctggcttttc ttcttcacaa
     5761 gtatctccat tggcgccatg ttgcttattg gcggaaaaca gggtctattt gctttaggaa
     5821 gtctcatcat caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag
     5881 ggatttctct attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat
     5941 tggctttaga tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg
     6001 tttttttagc attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc
     6061 gctatgagga actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac
     6121 tgattggaac agctggagga acactcgata ccgttatcac agtcattgcg acattagaag
     6181 aattgactaa gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag
     6241 tgggtcgaga tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt
     6301 cagttccatc gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac
     6361 agcatttatc attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga
     6421 tccctatctc ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat
     6481 tatacttgtt gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta
     6541 tattctttta ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca
     6601 tcagaagtgg ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat
     6661 cttatttttt atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat
     6721 ctttttattt tgtttcctac tattgcttcc tatcactgat tttttagcga tccaaggttt
     6781 tacatcggta gaactagaag aattatctgg cttagataag actttagcga ttgattttag
     6841 attacttacg agatcgttgc ttctgattag tcttagtggt gctgttttag atgctagtgt
     6901 agcaatttct agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca
     6961 attgcgccat tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact
     7021 tctttttgca ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat
     7081 tccttttcag caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct
     7141 taccacattg tctgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt
     7201 tacaaaaaag aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat
     7261 ttttactgaa tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa
     7321 ggaggcaatt tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct
     7381 ttcgactgaa gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca
     7441 aataaaaaaa gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca
     7501 attgatcgat aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga
     7561 aagagaaagg caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc
     7621 gaataaaact tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc
     7681 acgcaatgag aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt
     7741 gacagaagaa gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc
     7801 tagtggcgat acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg
     7861 taagaatcaa tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga
     7921 ttggaaagat caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg
     7981 atcacagtta ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga
     8041 caacatggaa tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga
     8101 gcatgaattt tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg
     8161 aaacgtgacg ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact
     8221 ttacggaaaa atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt
     8281 taatgaagat catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt
     8341 ctacttacct aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat
     8401 gattgaaaca ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt
     8461 ggaccaatta actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg
     8521 aaacgggaac atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc
     8581 gacagcagaa aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga
     8641 agataatttg aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc
     8701 agatggcatc ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct
     8761 gtccaattac gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg
     8821 tcgtgtttca gatggtgaaa tcacgcaagt gcaactttca acgacaacag gaagtatcta
     8881 tttaaaagat acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag
     8941 aagcgatttt gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgataggc
     9001 tatctcttga acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa
     9061 ggaataattt cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat
     9121 tttgcagaaa gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata
     9181 gtagaacaga ggtgagacat gatgaaaaaa aagttgacaa aatccaataa aaatgttgtt
     9241 ttgacaggga ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt
     9301 gtgctttatg tattcctgag cttagttgct tttggaagtc caatcatttt atacatttta
     9361 ttagctttga ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca
     9421 tattatgaaa aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa
     9481 gcggaaaaaa tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt
     9541 tagttaatac gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga
     9601 gcagtatttg gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca
     9661 aaccgatttt gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt
     9721 ttgttatcaa tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta
     9781 gtttttcttc attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa
     9841 ttgtcagtga acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt
     9901 tttttattta gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt
     9961 ttttaattgg tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga
    10021 aatgaggtag catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct
    10081 ttggaaatcg tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc
    10141 tcacgcccag gactagagtt gactggttat tttaattatt attcccataa tcgtttacag
    10201 ctatttggca gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc
    10261 atcatgagaa gattatgtag tgaagatatg cccgcattta ttatttcaag aggattaccg
    10321 gctccagaag aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc
    10381 atctcaactt ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct
    10441 cgaacaagtg tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt
    10501 gacagtggga taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg tcatcgattg
    10561 atcgcagacg atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg
    10621 cctaaaatcc tggaacatct aattgaaatc cgaggaatcg ggatcattga tgtaatgaat
    10681 ctttttggtg cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa
    10741 gcatgggcaa aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc
    10801 gcggaagtgg gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc
    10861 atcattgaag tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa
    10921 acatttgaag aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga
    10981 gtgacaatat gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct
    11041 actggtatgc attgatcatc gtatcaggta tcattatcgc catgtggctt agcagcagag
    11101 aggctgttcg ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc
    11161 ctatctccat tattggtgct cgcttatatt atgttttatt tgatcttccc cagtatatag
    11221 ctgatccaat ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga
    11281 ttgcaggcgc gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt
    11341 ttttagatat cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt
    11401 tcatgaacca cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt
    11461 taccgcaatt catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc
    11521 tttatgaatc actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac
    11581 cacacctttt gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc
    11641 gtttctttat tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt
    11701 ctcaattatt atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa
    11761 aaaaaggaaa tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat
    11821 ttttagagcg ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg
    11881 gttttaggac caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat
    11941 gacgttcgga tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg
    12001 aatcaacgtt atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg
    12061 gaagatgcag taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt
    12121 tctgttgcaa aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt
    12181 aaaggattag aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca
    12241 gcagaaaaaa gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagcg
    12301 aaacatgaca tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta
    12361 caagagctgt tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta
    12421 gagatggggg cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta
    12481 gggtttggcg ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt
    12541 ttaggtgtag caatgggtgc caacccgctg acctttatcg gattaagcgg agtaggcgat
    12601 ctagtcgtta cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga
    12661 caagggcagc ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat
    12721 actactcaag cagcgatgga actagcccaa ataatggaaa tcgaaatgcc catcactcaa
    12781 acaatctatg aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg
    12841 ttacgtgatg ggaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt
    12901 atatgaaagt caaaaaagca gttattccag cggcaggatt aggaacaaga tttttaccag
    12961 caacaaaagc tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca
    13021 tcgttgaaga agcgttgaaa tcaggaatcg aagacatctt gatcgttaca ggaaaagcta
    13081 aacgaccaat cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa
    13141 aaggaaagac cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca
    13201 tccgtcaatc tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg
    13261 gaaatgaacc gtttgtcgtt atgctaggcg atgatatcat ggaagatgaa gtaccattga
    13321 cacagcaatt aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag
    13381 ttcctcatga tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg
    13441 ggttatacaa tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt
    13501 tagctattat tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac
    13561 aaccaggagc cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac
    13621 aacgcgtatt tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt
    13681 ttatgaaaac aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg
    13741 agtatatcct gtctttaggt aaaaaattag aagcagaagg aagcaagatc gatcataatc
    13801 caaaaggaat aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct
    13861 tcagctgcta attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga
    13921 aaatgctatc cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa
    13981 attgcaggat tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg
    14041 ttgttgcaag tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg
    14101 acgatcgaaa tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttgctg
    14161 gtcaaaagta atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg
    14221 ttcacagcag ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac
    14281 atcgcgacga aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa
    14341 gtcggtggga cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga
    14401 aaataaaaat ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggaacagccg
    14461 cagcaatcgc cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag
    14521 ctgcccaagc ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa
    14581 aatcgactga gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag
    14641 gtgatctagc aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg
    14701 aatctgacga tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca
    14761 aaaaaacggc agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata
    14821 cttcagaaga tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca
    14881 atgaaggaat gtcagaagca aaaccagttg cagatgacac aaaagaaatg atgaaaatgg
    14941 caaaagaaga tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta
    15001 caaatcacta aaatcactaa aatcacaaat ttatagaata aaaaggtggg agcggactag
    15061 ctctcacctt ttttactctt atttttctaa aactagtaat tcttttggtg tagtcgtgaa
    15121 tggcacacag ccatcgcttg tcacatatac acagtcttcg atacgtaccc ctactttttc
    15181 aggaatatag attcctggtt caagagagaa gcacatccct tcttcgatta ctaagtcatt
    15241 tccttctaca agggaaggga attcgtggac ggttgttccg atcccgtgac ctaaacggtg
    15301 attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt gatcaagttc
    15361 acctgctgtc acacctggac ggacggcttc ttgtgcggca agctgtgctt caagagtgat
    15421 gttgtatatt ttttcttgga agtcggttgg tttttgataa gcaattgtac gagtagcatc
    15481 actgcaatag ccattccaaa caacgccaag atcgaataac acaagttcat tcggtgaaat
    15541 cttcgtgttt cctggtgtac catgagggct tgcagcattt gctcctgcaa ggactaatgt
    15601 gtcgaaagac atttgtgaaa caccttgttt tttcaattgg tattcgattt ctgcaatgat
    15661 cgcttgttca gtcacgcctt ctttcactgc tttaaagcca atctcaaatg caacatcggc
    15721 ccatttacca gcttcgatca gtttgtcttt ttcctctaga gttttgatca gttgtagacg
    15781 ttggatgatg gaagctagat cagtgtaaga atccgttccc gtcaagatat cagagatagc
    15841 atcgaagcgc tcgacagtca aggcgttctt ttcaatccct acattgccag gctgaccata
    15901 gcgagcacga agttcttttc cgatgatctc ccaagggttt tcgctgtcta aatagccacg
    15961 gacatcatac gcccaactac tgttttgagc atcttcaact tccaaagcag gagtgaaaag
    16021 gaaaggatct tgttcaagag ggataaaaag agctagtaca cgctcatgag gatcactttc
    16081 gtatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt cgacttggtg
    16141 ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc aacctacttt
    16201 ctaaattaat tttgtattca ataattgtac cactgattta ttaaaagacc aacgttctaa
    16261 aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca attcttgaga
    16321 ttatttgata ttctatttta gaaatgtaag acgtattttc agaaaacata taaaatatga
    16381 ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg agagaggcaa
    16441 atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtc aaaccagcaa
    16501 caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaac gcagttgcgc
    16561 gtggcttggc aagtaagaaa acaacaacag ttggggtaat catccctgat gtcagcaata
    16621 tgttctttgc ttctttagca cgtggtatcg atgatgtggc aacaatgtac aaatacaata
    16681 ttatcttagc aaattcagat ggtaacgacc aaaaagaagt caatgtattg aataacttac
    16741 tggctaagca agtggacggc gtgatcttca tgggtcacca tatcacagac gaaatccgtg
    16801 gagaattttc acgttcaaaa actcctgttg tattagctgg ctctattgat ccagatgaac
    16861 aagtaggtag cgtaaacatt gattatgtat cagcaacaaa agatgcagta aacaaattgg
    16921 caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc gttgatcgat cctatcaatg
    16981 gacaaaatcg tctgaaaggc tataaagaag cattaagcga aaataaccta ccatatagcg
    17041 aaggattgat ctttgaagca caatacaact tcaaagatgg tctatctcta gtcgatcgga
    17101 tccataatag cggtgcaaca gctgtctatg tttcagatga tgaattagcc atcggtattt
    17161 tggatggttt attagaccgt ggcgtgaaag taccggaaga attcgaaatc atcacaagta
    17221 acaattcact actgacagaa gtagctcgtc ctcgtttaac aagtatcaca caaccattat
    17281 acgatttagg ggcggtatct atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg
    17341 aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatctaca aaataataaa
    17401 aggacaacca ttcagaggtt gtgagcctgc cgtttaactc tgcgttacaa ggagcaattt
    17461 caaaaaacag catttcatgt ttgatgaata ttgtgactcg tgattcggag ttggcaggag
    17521 aacaccgttt attcgggaaa aggggttatg acaaaatttt gtttaatcct ttttttttgt
    17581 cttaaaaaag agagaaagtg gttgaaatta catttatcgt atataaaaca atccgtgtat
    17641 agatgctgca ttatgttcac caccatcaaa aaaataaggg actgtgtatt ctattaaatc
    17701 aatcatcttt ctcatctgat tctttggttg cttgattcgc tcttgcataa gctgaagcgg
    17761 tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta aaggcgcttt
    17821 tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt tggatggatt
    17881 ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact tttttcttca
    17941 tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt tttgcttggt
    18001 aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct ttgctgtcca
    18061 ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga gtagagacaa
    18121 ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa tctgcttttc
    18181 ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt tttgcattga
    18241 tcacactgcg catcatgtca ttcatgatcg atgccgttgc ttccgaatag atccggacag
    18301 agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa tcgatgatat
    18361 agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccattggtc tgttgaagcg
    18421 tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca tttgccgggt
    18481 agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg gaattcaaca
    18541 catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac tcacggacgg
    18601 tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca gcattttctc
    18661 cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata agccccatgt
    18721 cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga cgttctgtat
    18781 cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat ccgtagatgc
    18841 ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca gcaccatcat
    18901 ctaacatgta tccataattt tctacgccat tttgcaatgc agcatagaca tctttatcga
    18961 ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt tgataataag
    19021 cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat tgttgtgcaa
    19081 tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca gtctgttctg
    19141 cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct tcgtattgtt
    19201 ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt ctggcaaggc
    19261 cggcagaaag atcttctttt agcgcacctg tgttcgtgta tggactatac gtgatcgggc
    19321 tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt gcatctacac
    19381 caaagattcc tcttgcagct tcctggactc cagcaatgtt ttgtccctta ttattacgac
    19441 caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag tatttttcta
    19501 cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca tctgtaagga
    19561 tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca ataccagtag
    19621 cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt ttatcgaagt
    19681 attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatttta tcagatgaaa
    19741 tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct gcataaacaa
    19801 tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta gtcggcaatt
    19861 gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct agagctgcga
    19921 acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta aaaataatct
    19981 taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta tttttgcgtg
    20041 gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat aatagaacaa
    20101 aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta aaacgtgaga
    20161 aaatgatctt ttttataaaa gagttcttaa gcttttcttt agagaaaatt tcagttacta
    20221 tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta ccaatatata
    20281 ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt aggctcacac
    20341 gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc cagttatgga
    20401 tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa agccatcgaa
    20461 gagatgaata ttgatgccgg aaaagcgtac atggcagaaa acggtctaac aaatatcgtc
    20521 gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat gtttcctttt
    20581 gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc tactcaaatt
    20641 acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc tagaattatt
    20701 gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt ggagacaatg
    20761 gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat catcgatgga
    20821 gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa tgatgccggt
    20881 tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat catcgggtta
    20941 gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc gcataaagac
    21001 cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa gatcgtccat
    21061 gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtcgg caaagataaa
    21121 aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcaca aatattcgat
    21181 ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt tctatctgac
    21241 agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa acaaaggagt
    21301 gctttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat ttagacaaac
    21361 agggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg gcaaaacgtg
    21421 attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa ctaaaaaaga
    21481 aacatacata ctttattctt ggcatagccc tattatttac aattattcgt ttgtatttat
    21541 atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag ctgctgattc
    21601 gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact acaacacttt
    21661 ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat ctttcttatg
    21721 cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc gcgtttaaac
    21781 cgatcgtgaa gaacaagtat cttcttttaa gtttctattt ttattttttg ttttctccga
    21841 ttagttttac acatgaatat tccactcgaa cgtatcgtaa tgccctcgtg attccggctg
    21901 ttgagatcat tatcgcttgt ttactagcta tttattaccg taaatatagt tccagcaaaa
    21961 aaatgctgcc gtggttttta gggctagctg tttcttttcc tttcttttgg ttcattcgcg
    22021 aagattccat ctggcttctt ccttttactg tagtcgcgtt aggcatcagt ttcgtccaga
    22081 tcttgctagg acaagacttt tctttcaaag aagcaaagaa gttaaaagaa cgaccatttc
    22141 tcctttcgaa acaagaatgg ctgaaacttt cattgttttt actccctcta gtcggtttct
    22201 ccatcgtcta tcagtggatc gaatataaaa acgagacaac atatagtatt gccgccatca
    22261 atgaccgttc gagtggcgaa tttggacgtt tgatgaaaca gctgatccga atagatgacg
    22321 aaacgaactt aaataaagaa aacagtgaga tatgggtctc gcatgctgca ttaaataaag
    22381 cattagacgc atctcctaca tttcatgctg tttctactcg tattgacgaa ttgtatacga
    22441 ctcatgcgtg gacccaaggt ggaaaagtaa aagaattacc aggagatatc attttttggg
    22501 cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa gaaaccaatg
    22561 atttttggaa aaaagtcaat caagaattag aagaagccta tcaaaaggga actatcaaaa
    22621 agaaaaaaga attctatctg atgggatcag gggatggaaa gacgatgaat gatctgccaa
    22681 tcgtatttgc ttttctgaaa tcaggatatg cctatgcatg gcgatacaaa gatttccatc
    22741 aaggggggaa ttatagtttc ggtacgcaag aacaagtcga acaggcccaa gaattattga
    22801 atcttccact aatggataat tggatcgata gcagtgatcc gagaacacct gatttccagt
    22861 tgacaaaaac agcaaaagtc gcgaatgtca tgataaaaat ttaccaagtt ctggccattc
    22921 ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc gcatttgcaa
    22981 aagctgatcg tgcctattat agttcctctt tgattgtagt caccggatta atactgacac
    23041 aattggtttt tcttttcggc gtctcgtggt tttgttctta tgcgccagaa aaaaaggatt
    23101 attttttaag tgtctacact ggcgcaggcg tgccattaac gcaattcatc gtagtaacaa
    23161 gcatgctagt tttcagtaaa ttgaatctga aaaaagcttt atcttccaaa taaaaacata
    23221 ctaagattag aggagaacat cattgacgaa tgatgttctc ctctattttc tatttttaga
    23281 ttatagtgaa gagtgaaaga aagttcgaac aaagccttga ggactatgag tccctatcaa
    23341 aaaccgaact tctttactgt cattttagaa tcaggtttac gtatgttggg gccatgagcc
    23401 cgtgtatagg aaattggaat cgaaatgaag cagtaaagtt ctttcaaaac ttaaaaagga
    23461 ggaaaacaaa tgccttacat tcttgcatta gatgtttcaa tgggacacag ctacagtgtc
    23521 ctatacaaag acgacacctg cttatttgaa gacgagatcg aacacactca aagaggattt
    23581 catgcattac tcgaagagat ccagcacttg cctgaacgtc cattgattgt ttttgaagca
    23641 acggggattt attccagacc agtcgaaaag ttctttcaag atcatcactt ttcttactgt
    23701 ttgttaaacc cgttagaagc gaaaaaacag atggaagaaa acaccttatg cagttggaaa
    23761 acagataaat cggatgccca tcggctcgca cagactcatc ttaaaaatca acgtcagcca
    23821 aaagaagtac aaagcaattt ttacttagag atgcgcgatt tagcccgttt ttatcaagaa
    23881 attgagaaga aaatcactcg tctgcgcatg gatttacaca actgccttca gttgactttt
    23941 ccagagcttg aacagttctt tttaaacagg ctgacccctt acgcattgat attgatccgt
    24001 ctttttcccc atccagattt tgtattggca tctactcgga caaaaataaa aaataaactg
    24061 atcaacgaaa cacggaaaaa aatctccgcg aatcgtgccg agcaaaaggc agatcaaatc
    24121 atccactatg cacagtgtgc ctatccagct gttgaaaaag acagtatcca ctgtcaaaaa
    24181 acgatctatt acgctgaact tctccaagat ttactcgaac aaaaagaagc actggcgaca
    24241 caaatgatca aaaaagccga aggctctcct tgctttttgt tgtaccaaac gttccctggg
    24301 attggcgcac tcacagccgc tttattgctc ggtgaattag gagatatcac acgatttaaa
    24361 acccataaac aattgaatgc ctttgtcgga atcgacatcc gacggtatca ttcaggtaag
    24421 tatactggcc aagaccgcat caataagcgt ggtaatccca aagcacggaa aattattttc
    24481 ttcaccattc gaaatatgat tcgccagcaa cgcgcagctc ctaatcatat tgtcgattat
    24541 tactacaaat taaaaaagca acctatccct aagaaagata aggttgccac agttgcttgt
    24601 atgaacaagc ttctgaaatg tatgcacgct atggttaggg cacatacaga gtacgactat
    24661 gcgtacgcgg tctcagtgga ccattaacta aaccacttac agtataccac ttctttgccc
    24721 agatcgaaaa ttttttacga acggggcttt atttggtgtg ccttgatttt gtcgctttcg
    24781 tttatgagct atttttctta caccttttac ttgactaatc gtaggaaagg gaactgtgac
    24841 aaaagtcttg tcgcagttcc tttttccgaa taaacggtgc tcaaaagctt gcatctgcgg
    24901 taataagtcc aaccaagtta aaaacataaa aagctgtttt tacttggctg ttcttattac
    24961 ttgatgcaag gcagcttttt cacaacctct gatacacggt gttccagaag taacttcttc
    25021 ggaaataagc cgaaatttca caaaaatttg aaaagcaatt ttcttaaatt ccttcttatt
    25081 tcttgaagct gcccacttct gacacaacct cctttgtgta aaagaatcag tttctactcg
    25141 atttcttcta tcgtttcctg caaacgctgt tcatattttt tgtttccttt atataaatca
    25201 ccaatcgtcc atgccaacag gagaaggaca agaacaatga ttacataagc gatttgatca
    25261 gccaatgcta gttgagctga ggtggcttgc tcgccgaaga aactttcgat ttgacctggt
    25321 aatcctcgta aattcaagta agtcagacta atcacagaga accagccaag tagttttaca
    25381 acaaatgaat ttttgaatcg gctccccatc tccgcttggc tattggtcat catcagtaaa
    25441 ggaatcatcg agaatggcaa tgcaaaagcc agaaaaactt gggattcatt catcaagtta
    25501 ttcaaagcgg tatgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat
    25561 acaggaatca cagaaagcaa tcgtgtgact agtcttctta gccaaattgg gatacgcata
    25621 tggatgaagc cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg
    25681 ccagaagcaa gaagtgctac agcaaataaa gtagacaaga taccagattt tgctacagca
    25741 atcaagatac cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag
    25801 gaaggatctt ggacagaacc ggttttgaat acagccacac ccatgatcaa tagcagtgca
    25861 ttgactagaa aagccattga caactggata ttagaatccc aagtagaaaa gcgtaccgct
    25921 tgtgctacat cttcttcgtc ttggtgatcg atcttccgag tttgagaaac tgctgaatga
    25981 agatataagt tatgcggcat gactgttgca ccaataatcc ctaaagctcc agtcagcgga
    26041 gtttcgccgc cgatgaccgg tgagtctgag aatgtgtgac tgttagggat aaatccggca
    26101 aacaaactgt gccaatcagg gttagacaag ccaacttgat agacgaaaac gaataaaatg
    26161 acgaaaatca agcagacaac caaagcttcg attttacgaa acccgacttt tgtcagcaat
    26221 aaaaggacga aaacatctaa cacagtgata aatacggcaa ttaccaatgg aatatcaaaa
    26281 agcaggtaga gggcgattgc tgcaccaatg acttctgcga tgtctgttgc cataatggct
    26341 aattcagtca agatccataa aatgacaccc aatgtcttac ttgtccgtgc tcggattgct
    26401 tgggccaaat ccatttgggt cacgattcct aattttgctg ccatatattg taaaagcata
    26461 gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt ttgtccacca
    26521 gtgatcgaag tggaccagtt acctggatcc atataaccaa ctgctactaa tgcacctggt
    26581 ccagagtaag caagaagtgt tttaaaaaaa ctttttccct tgggtacatc cactgttcca
    26641 tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt
    26701 ttgttggatt gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta
    26761 cacctcagaa agaaaaaatt aaagaaaaag atttggtatg cctaattttt tttcgtggcg
    26821 tgtcttgctg gttaatgaaa gaaattttgt tcttctctaa aaaaaccact attttttgca
    26881 tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa atcattgaga
    26941 gcaattcgga aatagaaagg agagatttat atggtatcaa caggtattgt cgtacttatt
    27001 gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg taaatatatg
    27061 aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg
    27121 tcaatggggc aaaaaccatc tgagaagaaa attcgccaga tgatgcagca aatgaagaac
    27181 caagggaaaa aataggttct gacccaaaga ctttttgcgg gctgattttt tcagtccgtt
    27241 ttttatttcg taatttgtta gactattcag actattcagc aaaaaagaca agcacagaca
    27301 atcctatgct cttgataaag acaaaggagg agtaaaatgt cgatttttag aaaattaggt
    27361 tggttttttc gacaggaaaa gaaacattat ttaattggga ttttctccct attcgcagtg
    27421 gccttggtac agttagctcc tccaaaagtg attgggatca tcattgatga gatcgccgat
    27481 aaaaatttat caataaagat catttcctgg tggatcttat tattgatcct tgcagctctc
    27541 ttacaatatc tttttcgtta tatctggcgg acgaatattt ggggaagtgc agctcggttg
    27601 gagaaagaac ttcggcagca gctattcaaa cattttacag aaatggacaa tctcttttac
    27661 caaaaatatc ggactggaga tttgatggct catgcaacga atgatttgaa tgcaatccaa
    27721 aatgtagcag gagctggtat cttgaccttt gcagattcag tgatcacggg cggaacaacg
    27781 atcattgcga tgattttgtt tattgattgg cgcttaacac tgattgcgct gattcctctg
    27841 cctttactag ccgttacttc acgagtgctg gggtcaaaac tccacgatgc ttttcgtgat
    27901 tcgcaagaag ctttttctag tatcaatgat aagacacaag aaagtattac tgggatcaaa
    27961 gtcattaaaa catttggaca agaaaaagaa gatctcgcgg attttgaaga aaagattgaa
    28021 gatgcaatta ttaaaaacaa acgagtcaat tttttagact ccttatttga tccattcatt
    28081 accttgatta tcggtctgtc ttatgttgca acaattattt taggtggaaa gtatgtcttg
    28141 gaaggcacga tctcaatcgg gcagatggtc tcatttatta gctatatcgg tatgcttgtt
    28201 tggccgatgt ttgcgattgg tcgattgttc aacgtattgg aaagaggaaa tgcttcttat
    28261 gatcgcgtca atgaactttt gcatgaaaag acgcatatcg tggaacaact gggtgcagtg
    28321 cagactccgg caaaaggcga gctgaatgta gctatttcct cttttaacta tccgaaagaa
    28381 gaatcaccag cattagaaca aatcaaattt gcgatccaag aaggagaaac attaggtatt
    28441 gttgggaaaa ctggtgcggg aaaaacgaca atcatcaaat tactgatgcg tgaatacgat
    28501 gattatcaag gaaaaattca atttggcgga tgttcgatca aagaatattc attgaatgct
    28561 ttattaggat caatcggcta tgtgcctcaa gatcacttct tgttttcgat gaccattcga
    28621 gataatatcc gtttttctaa tccaaaactt tcagaagaag aagtagagca tgcggcttct
    28681 ttagcattta tcaacaatga aatcaaggaa ttaccaaaag gttatgatac cttagttgga
    28741 gaacgtggag tctctttatc tggcggacag aaacaaagga tttcgattgc tcgagcattg
    28801 atcaaaaatc ctgaattgct gatcttagat gatgcacttt ctgcagtaga tgccaaaaca
    28861 gaagaagcta tcttgcataa tttaaaagaa ataagaaaag agaaaacaac catcattgca
    28921 gctcaccgtt taagcagcgt gatgcatgca aaagaaatca ttgttgtcga tcaagggaaa
    28981 atcattgaac gaggaaccca tcatgagctg cttgcattag gcggctggta tgaacgaatg
    29041 tgggagaaac agaaattaga agcgaagata gaaggaggcg aggcctgatg gatgaaaatt
    29101 atcaatcgga atggacaaaa tcgattccac ttaaagaaca gaagaagatc atcaaacgac
    29161 tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt tgctatctta tttgcgtttg
    29221 ccctgtctgt gatcaatgtc ctattgccaa ggatcatcca aacttttatg gatgatcatc
    29281 tggctaaaca gtcggctacg acacaagtta ttttgttttt tgctggtatg tacttgtttg
    29341 gagtcatagt caaaagtatc atttggtttt tccaatggta tttgtattcc atggcttcat
    29401 tgaagaccta tcagtatgtt cgggtaagat tatttgaaaa gctgcataca ctaggtatgc
    29461 gttattttga tcagacaccg gcaggatcga ctgtttctcg agtgacaaat gacacagaaa
    29521 cgctatttga attttggtat gtctttttga tggtattgac tgggatcttt gcagtcgttt
    29581 cttccttcat tgccatgttt caaatcaatg gagaaattgc cttgtggact ttggtgttct
    29641 tgccgatttt aggaatcgtc atctggtatt accagaaatt cagttctcgt atttaccgga
    29701 atatgcggga aaaattaagt cagctgaaca cgaaattgaa tgaatcgatt tccggcatgc
    29761 gtattatcca gcagtttcgc caagaagctc gtttagctaa agaatttgaa caagtgaaca
    29821 acgagtattt agacacgcgc tatgcgatga tcaaaacaaa ttcgattctg ttaagtccga
    29881 tcatcaattt tctatatgca ttggcgattg cgttggcgtt gactttgttt gggatcgatg
    29941 cgctgcattc tcccgtagaa gttggactga tctatgcatt tacgacctat gtccagggat
    30001 tttttaatcc aatgtctcaa atgatggact ttttgagcac ctttacagat ggaatggttg
    30061 ccggtagtcg tattttgaaa atcatggata cagaagaata tgcaccgcaa caaaatcctg
    30121 gagcagatca agcgattact tcaggtaaga tcgagtttcg caatgtaagc ttttcttatg
    30181 atggagagca caatgtattg aacaatatct cttttgtcgc taatccggga gagaccgttg
    30241 cattagtggg gcacaccgga agcggaaaaa gctcgatcat caacgtattg atgcggtttt
    30301 acgaatttta tgaaggccag atcttgattg atgatcatga cattcgagaa tatccaatgg
    30361 aagagttacg taagaaaatg ggattggtct tgcaagatgc cttcttgttt tatggagata
    30421 tcgcaggaaa tatccggatg atgaatccaa ctatcacaga cgaacagatt cgccatgcag
    30481 ctgagtttgt tcaagcaaac aagttcatcg atgaattacc aaaaggatat cactcaaaag
    30541 tgatcgaaca cggtgccagc tattctagcg gacaacgaca gctgatcact tttgcgcgaa
    30601 caatcgtgac agatcctaaa atactagtat tggatgaagc gacagcaaat atcgatactg
    30661 aaacagaagg attgatccaa gaaggattga caaaaatgcg acaaggacgg acaacgattg
    30721 cgattgccca tcggctgtcc acgattcggg atgcagaatt gattttagtt ttagacaaag
    30781 gtcatatagt agaacgtggg aatcatgaaa cacttctagc gcaaaatggt ttgtatgcag
    30841 atatgtacaa actgcaaagc agtggtgtgt aaaaagtggt tgtgacaggg ctccgttcag
    30901 ctttaggtga taagaaaaaa attgtaaaaa aggctttcca gatttttgca taaatttggc
    30961 ttatcaccga taagctggta ctggaacacc gtatattaga agttgggaca gtagcatagt
    31021 agcattttga cctaagcgct agagtgaaaa taaaacagag cttcccagat tcttcatctg
    31081 tgagcgttag tgtcgtaatt accagattta aaaaaaatca cgacagctag tcagaagtgc
    31141 ccaatagccg ctaagaaagc gttgacaaaa gtgcttgctt tcgataatat aaagaaaacg
    31201 atacaacttg gatagtgatt gcctgatgca agctaaacct gatgagtctc ccatggttcc
    31261 atggacggct ttttgggttt ttctttttgt tttggataca gccctatata tcatggaaag
    31321 gagtaaaaca tgctgattga gaaagtactg aataacaacg tcgtaataac cagggatgaa
    31381 agaaatcagg aaatgatcgt gatgggaaaa ggtttggcat tcaaaaagaa agtgggtgac
    31441 ccgatttttg actcatgcat cgacaaaaaa tttcagttga caaatgacgg actttcacaa
    31501 cagttccaag aattggcaca agcgattcct cttgagtact tagaaatttc ttgtgaaatc
    31561 attgaatata caaaagaggc tctacaagtc cagttgaatg attcggtgta tattgcattg
    31621 acggatcatt tgtatacggc tatcgaacgt cacaaaatgg gaatatttgt accaaatgta
    31681 ttgctgtatg atatcaaaca actatttcct aaagagtatg ccatcgggaa aaaaacagtg
    31741 aagaaaatct cggataaata ccacatcaag ttatcagaag atgaagccgg atttatcgca
    31801 ttgcatttag tcaatgcaca aggcattcaa agccaacaag acaataccta cctgtttact
    31861 acaacaatac aggatattct aaatatcgtc cgctattact ataagattac atttgatgaa
    31921 gattcagtcc atttttttcg attcgtcaca cacctacgtt tttttctgac acgcgtccta
    31981 gatggagaaa aatcagaagt gggagaagtt tctgaggaat tgtaccaggt tattcaaaaa
    32041 aaatataagg atacgtttgg ttgcgttgag aaaatagcag actttttagc cgagatattc
    32101 aactaccaga tgtccacgga cgaaaaaatg tatttaacga tccatattgc gcgtttagtg
    32161 catgagaatc gttgtaaata aataaaaaaa ggaggataaa ttaacaagaa cgcatgaaat
    32221 aagggtagtg acattcagtt ggctaaacct tccactaata aattggaggg aatagaaatg
    32281 ggaaaatatc aagaattagc gagcaaaatt gtagaaaatg tcggcgggaa ggaaaatatc
    32341 aatggtttga cacattgtat cacaaggctt cgtttcaaat taaaaaacga agaaaaagca
    32401 aatgatgaga tcttgaaaaa tatggacggt gttgtgacgg taatgcgagc gggaggacag
    32461 taccaagttg tcatcgggaa tcatgtgcca gtcgtgtttg aagaggttat aaaagcagga
    32521 aaccttactt ttgatgaggc tgtttcaaca gaaaaaatga gaccgtttga tatgcttatt
    32581 gatggaatat cgggctgttt ccagccattc cttgctattc tagctgctgg tgggatgatt
    32641 cgtggactga cagcattact tgtcttctta ggcgcatttg accgcggttc tggcacattt
    32701 gttatgtttg ataatatcgg tgattctgta ttccaattta tgcctgttat tatcggttta
    32761 acagctgccc gtaaatttaa agtaaatgaa ttcgtaggga tgttgcttgg ggcagcatta
    32821 atgaatccta gcttgtcgct ggaagctttg agtggtgcag cagaagcacc actgactacc
    32881 atattttctg gcactatttt tgaagcacct atttatcaaa cagtattcgg cattccctgg
    32941 attgcgcgta actacgcttc ttctgtcatt cccattatct ttattgtgtt gttagctagc
    33001 caaattcaga aaccaattaa aaagttagta cctgagatga tcgcgaattt cttcgttcca
    33061 ttcttcaccg ttcttattac aatgccctta ggttttttac tcgttggacc agcattcaca
    33121 ttcgcaactg atattttaat ggctggattt gaaacacttt tagcactttc gccagttatt
    33181 tatggagcaa tcgtaggctt cttctggcaa attctagtga tgtttggtct ccattgggca
    33241 atcgtaccaa tgggattgat gcaattttct gtgaatggtt ggcaaaatat catgacacct
    33301 gttgcagtag tgtcttttgg acaaactgca gcacttactg cactctactt taagcttaga
    33361 aacccaaaag acaaagcaat cgcaatacca gccattgttt ctggtattgt ggggattaca
    33421 gagccagcta tttatgggtt tacattgcca cgtaaaaaaa tatttatttt tacctgtgtc
    33481 ggtggtgcgt tagggggcgc gtatagtgga ttaatgaatt taacttcttg gaatccaggt
    33541 ggattaggca tctttacaat tcccaactat attagaccag atggtgattt aacggatgtg
    33601 attaatgtat tgattggaat cgccattgcg atggtggtat cctttgcgct gacttttttc
    33661 ttttggaagg aggaagcagg tgaaacagat gatagacaga aaagatcagg taaagaaatt
    33721 gtaaaaacac cgatacaagg acaaatcgct ccactgaatg cagcaaagga tgcagctttt
    33781 gcacaaggaa cactaggtcg aggaatcttg atctaccctg aaaaaggaga agtacgtgcc
    33841 ccttttgacg gaacagtcat gaccctgttc ccgacgaaac atgcaatcgg aatggtttct
    33901 gaagcaggtc tggaattgct gattcatgtc ggattggata ctgttcagtt ggaagggaaa
    33961 tattttgaaa gcttggtgca gcaaggagaa agagtgaaac aaggcgatct gttgctccgc
    34021 ttcgacaaaa agaaaatcga agaggaagga tacagtttag aaacacctgt gatcgtcacc
    34081 aattacacgg attatttgga tattttggaa aatcaagggg agacggtcgg gccagacgat
    34141 gcacttatca ccgcactgac ctgaacggtt aggtgaaaga atggactagg cttaattatt
    34201 ctttttagtc cacttcttta ttttcaaaat tgaaaggctc tttcatgatg gctgagctga
    34261 tagggcgttt atcaaatttt tgtcctacag aaattagatg gactaaattc tgttgataat
    34321 tttctgcaaa aacacaacta tataacacgt ctagaagata acaaatcgat gtattgatcg
    34381 taaaattacc aattttcgaa tacagtcgtt cgcgtgtcgt aagtcgcaaa gcggctgtac
    34441 tcattgaaga tagcgttgaa tcaccgatac tggtcaaagc taaaattgga acatgatttt
    34501 gtttcaatat ttctgcggta cgaaggatca cattgttttc acccgtataa gaaatcagaa
    34561 tcccgcacat atcactcgtg caattgtacg cttcatagaa cgattcacca aaagtggtga
    34621 caatcgtgac atccattttg atgcgcttca ttttcagcat gaaatcttga gaaatcaaag
    34681 tattcgagtt actggcgaaa atcacgattt tttttgcttg aagcattagt tgtttggctt
    34741 tttgcagttg gtcatgatga atgagagaaa gtgtatcttg aatcgtcatc tgttctaaag
    34801 aagccatttt atttgcgatc gtcataatac cgtctgtggt ggaaaaagga agattcgcat
    34861 ctacgtcttg aaaattccct tcaagatagg ctgtttcagc taaaaaagca ttacgaaaac
    34921 tgacccagcc atcaaaaccc attttttttg ctacacggat gagtgtggac gggtggacat
    34981 aacatcgctc agcaatctct tggatggtca tttcgccaag tgctggagaa tcgcttaaga
    35041 gaaactgaat cacttcattt tctgaaggag aaaaggagat ttctttgatt ttttcacgta
    35101 ataacatgta gataccttct tcttgttttt ttacgtaaat tataactttt ttgaaaatga
    35161 tttgtaaacg gatttatgtt atatagctta ataaagacat tttttgacaa gatatttaca
    35221 aaaatccagt agaaaacact aacttttgta aatgttactg gaaagaaacg aatttctatt
    35281 gtttgttttg tttacaaaaa agataataaa agtgtaaaaa gtaaaggagt gagaacatgg
    35341 caaaaggaat caaaatcgct acgattggtg gaggcagcag ttacaccccc gagttgatgg
    35401 aaggatttat caaaagatat gaggaattac cgatacgaga aatctggctc gtagatgtag
    35461 aagaaggaaa agaaaagctt gagatcgtca gtcagatgtc tcagcgtatg tgggatgctt
    35521 ctccgtatga agtcaaaatc tatgcaactc tggatcggga agaagcattg aaagatgcgg
    35581 attttgttac gactcagttt cgtgtcggtc aattagatgc acgaatcaaa gacgagcgga
    35641 tccctagtta ttatgggatg attgggcaag aaacaaatgg tgctggcggg atattcaaag
    35701 cttttcgtac cgttccagtg gttttggcaa tcgttgaaga catgaagcgt ttatgtccaa
    35761 atgcttggct gatcaatttc gcgaacccaa gcgggatggt gacagaagca gttatcagat
    35821 atggcaaatg ggataaagtg ataggattat gcaatgtgcc agtaatggcg atgatgacag
    35881 aaccaacaat gcttggcaag aaaaaagaag agctgatcta caagtttgcc ggtctaaatc
    35941 acttccattg gcacaaagta gcagataata aaggaaatga ccgcacggca gaattgattg
    36001 atcgtttata cgatgaagac aatggattgc cgaaaaatat ctttgatatc ccgtttttca
    36061 aagaacaatt aaaacaaatg aacatgatcc cttgtggcta ccaccgctat tactaccgtg
    36121 aagaagaaat gctggaacat gctttagaag aatatcaaac gattggtaca agagcgcagc
    36181 aagtgaaaga aacagaagca gagctgtttg aattatacaa agatccagaa ttggatcata
    36241 aacctgaaca attgcaaaaa cgaggtggtg cttattattc agatgctgcc tgtgaaacga
    36301 ttgcttcgat ctatggaaat aaagaaacac agatcgtggt atctacaaag aatgatggag
    36361 ctgttcctga tctgccggca gattgtgtag tagaagtcac tgcctatgtt ggcgcacaag
    36421 gcgcacgaaa tgtagcgttt ggttcccttc ctgctgctga aagaggctgg ttgcaagtaa
    36481 tgaagaatat ggaattgctg acgatcgaag cagctgtgac tggtgattat ggaacagctt
    36541 tacaagcgtt tacgatcaat ccgcttatac gatcaggaaa gacagcaaaa cggatcatga
    36601 atgagttgtt tattgcacat cagcagtatt taccgcaatt caaggaaaca atcagccggt
    36661 taaaagcaga aggaatcgaa gtccaagatg aactggcaag agaattatca agcaatacac
    36721 atgtaatgga ggaagtatga tgggattcag aaaagatttt ttatggggcg gagctacagc
    36781 agccaatcaa tgcgagggcg gttatgatga aggcggacgt gggttagcga acgtagatgt
    36841 tgtgccaatc ggagaagacc gttttcctgt cattactgga aaaaagaaaa tgtttgaatt
    36901 tgatgaaaat catttttatc cagcaaaaat tgcgatcgat atgtatcatc attataagga
    36961 agatattgct ttatttgcag aaatggggtt caagacttat cggatgtcca ttgcttggag
    37021 tcgtattttt ccaatgggcg atgaaaaaga gccaaatgaa tctggtttga aattttatga
    37081 ggatgtcttt aaagaatgtc aaaagtacgg gatcgagccg cttgtgacga ttacacattt
    37141 tgattgtccg atgcatttga tcaaagaata tggtgcgtgg cgttccagag aactcgttgg
    37201 tttttatgaa aatctgtgcc gagtcatctt caatcgctat aaaggacttg tcaaatattg
    37261 gttgacattc aatgagatca atatgatcct tcatgcacca ttcatgggcg cagggctgta
    37321 ttttgaagaa ggagaaaatg aagaacaaat caaatatcaa gcggcacatc atgagttggt
    37381 tgcaagtgcc atcgcaacga agattgctca tgaagtagat ccggagaaca aagtaggctg
    37441 tatgctggca gcaggagcat attatccata cacttgcaat cctgaagatg tttgggcttc
    37501 tagaaaatca gatcgagaaa attatttctt cattgatgtc cagtcacgag gagaatatcc
    37561 tgcttatgca ttgaaagaaa tggaacgaaa tggcattcag atcaaaatgg aacctgggga
    37621 tgccgcattg ctgaaagaac atactgttga ttttatctcc ttttcttatt attcttctcg
    37681 agtttcgact actgacaacg aactcttgga acagactgca ggaaatatct ttgcttctgt
    37741 caaaaatcca tatctggaag caagtgaatg gggttggcaa attgatccat taggcttgag
    37801 aataacgatg aatgatattt atgatcgtta tcaaaaacca ctatttattg tagaaaacgg
    37861 cttaggtgca gtcgatacgc ccgatgagaa tggttatgtg gaagatgact atcgaatcga
    37921 ctatctggca gcacatatcc aggcaatgaa agatgcagta gaattagatg gagtcgatct
    37981 attaggctat acgacttggg gatgtattga tttagtatca gctggtacag gtgagatgaa
    38041 gaaaaggtac ggattcattt atgtcgatcg tgacaacgaa ggaaatggca cattagcccg
    38101 ttcgaagaag aaatcattca actggtataa aaaagtaatt gattcaaatg gagaaaatct
    38161 ttcgaatgat tgatcaatga tttataaaag tctattgttt ttcagcggat ttttcgagta
    38221 taataaagct attcgagaaa cgcaagcaga acaatagact ttttttcgca ttggctgtaa
    38281 agtatgctta cttgattaag taaaggagaa tctacatatg tatgatgtaa tcgttattgg
    38341 cgcaggtcct gctgggatga ctgctgcttt atatgcttca cgttcaaact tatccgtatt
    38401 gatgatcgaa caaggcgccc ctggcggcca aatgaacaat acggcagaaa tcgaaaacta
    38461 tcctggtttc gattcaataa tgggtcctga tctagcttat aagatgtatg aaggtgtttc
    38521 aagatttggg acagaaaatg cttatggaat cgtgcaagat atcaaggatc acggcagcca
    38581 caaagaagtt atttgtgaag ataaaactta cgaaggaaaa acagtcatta tcgcaacagg
    38641 ctgtatccac cgtaaattag gcgtacctgg cgaggaagaa tttgctggac gtggcgtttc
    38701 ttactgtgca gtttgtgatg gtgcgttttt ccgtaacaaa cgtctagtag tgatcggagg
    38761 aggagactca gcagtagaag aagccatcta tttgacacaa tttgcttctg aagtagtcat
    38821 cgtccaccgt agagatgaat tacgtgcaca aaagatcatc caagaccgtg catttgcgaa
    38881 tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa atcgttggga atgaaatggt
    38941 agtgacaggt gtgaaagcac gtaatgtcaa aaccggcaat gagtttgatt tggcagcaga
    39001 cggagtattc atctatgttg gactggatcc attaactgca ccatttagag aagcagggtt
    39061 gacgaacgaa gctggctgga ttccaacaga caacgaaatg aagacgaata taccaggcgt
    39121 ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa atcactactg ctgttggtga
    39181 tggtgggatc gctggacaac aagtgtataa atacttggaa tctttagaag aaacaactgc
    39241 ataagtttaa aaagagtgga agaacggttt aattgttctt ttactctttt ttttcgcgtg
    39301 aaatctactg aatccgattt atgcgaatac ttgcaaccgt gcttgataaa agaatccttt
    39361 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    39421 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    39481 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    39541 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggc
    39601 atgcggggaa tcttagggcc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    39661 acagaaggac ttgcacgttt catggataca caaggcgaag aaacaaaacg ccgaggtgtc
    39721 gctatcgctt atgactcacg ccatcaatca ccagaatttg ccatggaagc agccaaaaca
    39781 ttagcacatc acaatatccc atcttatgta ttcgaaagtc tacgtccaac accagagctt
    39841 tcttttgcag tccgtcattt gaagacgttt gcgggaatca tggtaaccgc atcccacaat
    39901 ccagcagctt acaacggata taaagtctac ggagaagatg ggggacaaat gcctcctgca
    39961 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgttaaa agttgaagta
    40021 ttatctgacg aagaagctga acatagcgga ttgatcacga tcatcggaga agaagtggat
    40081 gccgcttatc tagaagaaat caaagcagta acgattgatc atgaattggt ggaaacaatg
    40141 gggaaagatt tgaaacttgt gtacacacca cttcacggaa caggaaaaat gttgggcgaa
    40201 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttccagaaca agctgtcgca
    40261 gacccagatt tcactacagt caaatcacca aatccagaag aacactctgc ttttgagtat
    40321 gcgatacgac ttggcgagaa agaaggcgct gatttgctga ttgcaacaga tcctgatgca
    40381 gaccgtttag gtgcagccgt tcgtttgcca aatggctcct atgaagtatt gaccggaaac
    40441 caaatcggag cactgtttgc tcaatatatt ttggaagcac accaatcgcg aggcacttta
    40501 cctgaaaacg cagcggtatt gaaatcgatc gtttctagcg aattacctac agcaattgcg
    40561 gaacactatg gggctaccat gttcaatgta ttgacaggat tcaaattcat tgctgaaaaa
    40621 attcaacaat ttgaagaaga tcattcccat acttttatgt tcggattcga agaaagttac
    40681 ggttatttag ttaaaccttt cgttcgcgac aaagacgcga tccaagcatt gctcttatta
    40741 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgatgg cttgcaaacg
    40801 atttttgaaa cctatggcta ctacgcagaa aaaacgattt ctgtgacaat gagcggacaa
    40861 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtgaagaagc accaacagaa
    40921 tttgccggag caaaagtcat ccaaacagaa gacttcaaag agttgacgag aacagaccgt
    40981 gaaggaaacg tagaaaaact aacgacaccg ccatctgacg tattgaagta tacgctggaa
    41041 gaaaatggct ggatcgctat ccgtccttca ggaactgagc cgaaaatcaa attttatatc
    41101 ggtgtaaaag gaaactcttc agaagacgca gctgaaaaag tagccgctta tgaaacgatt
    41161 atccaaaaga tgacagaaga ataaaaaaac ttgagactga tggaaaaagt ggtaaacttt
    41221 agaagtcagt cttttttcaa gttggaggtg aatggattgc cagacacgca aaaaacaaaa
    41281 gtagagaaag tcagccaact attcaaaatg ttgagtgacc ccactcgtct caaaattctg
    41341 ctgtatctga aagacgggga acaaaatgtg acagcaatca tccaagcagt ggaaatggaa
    41401 caatctgctg tatcgcatca actgcgttta ttaagagaaa accatgtcgt caagtcccat
    41461 cgtgagggaa aagcgatttt gtatagtctt gatgattctc acgtactgga tattttgaac
    41521 cagacactca aacatgtgga acatcaataa cgataggtgc aaatagagac aaactggact
    41581 gtgtcaacga atcttcttcg ctgacacagt ccagtttttt taaagaaaaa ttgttagtct
    41641 gtttcttcgg tggtttcagt tccataataa ctatggtctt ctaggtctaa agcattcaaa
    41701 ataagcgaag ctgtttcttc aatagataaa gaagcgacat tgattactac gcagcccaat
    41761 ttttcataaa gatcattcgc aaaatcgagt tcagctcgta ttttttcgat atcggaataa
    41821 gcagtatcag gatttaatcc gtaagagcgc atccgttctt tacggatatt gtttaaaata
    41881 tctgggttat tcgtcagtcc cacgattttt ttaggatcga tctcccaaag ctgtttggga
    41941 atatgtgctt gtgggatcaa aggtagattg gcaactttta aatttttgtt agctaaaaac
    42001 aagctcaaag gcgttttgga agttcgggat acgcctagca aaacgacatc tgcttctaga
    42061 aacccacgag gatctttccc gtcatcgtat tttacagcaa attccattgc tttgatccgt
    42121 ttgaaatagt tctcattcaa taaatgaagg gcgccaggtt gccgaatggg cttgatacca
    42181 gttaagcgct cgatttctga tatcggcgga gtcatcacat caaaactaaa taattgatgt
    42241 tcttcacaaa aagaacgcgt catctcgatc aattcttgat tgatcaatgt gtgcaggaca
    42301 agagcttttt cttctagtgc atcttgcagt gcctgcatca atgttttctc atctgtcaca
    42361 aatgttctgc ggaataaatt gaactcaacg ctaggatatt gggccatagt ggcttgcgct
    42421 agttttgtcg cagtttctcc agcagagtcg gagatgacaa aaaaagtaac gatttctgta
    42481 gaatcggaag acatggataa aacctcactt gcattttttt tatgtttatt ataccatact
    42541 ctagaatgtg tacttcgtaa tcattttgat ttggaggaat aagtcatgaa aaaacagacg
    42601 ttgatcgaac aatcactaga aacattgaaa aataacggtt ttaaatatac taaaaaaaga
    42661 gaagcattgt taacttattt agtgaagcgc aatcgctacg tatccgctaa agaagttttt
    42721 gactatatga atcaggaatt caaaggagta agttacgata cgatttatcg aaatctccat
    42781 gattttgcag aaatcgattt actggaagaa accgatttaa atggagagat gaagtttcgt
    42841 tttcattgct gtcaagggga aatcggccat caccatcact ttatctgtac ggtgtgcgga
    42901 acaacgaaag aaatccatat gtgtccaatg aattatttcg aagaacagct cggtggatgt
    42961 acgatcgaag ggcatagatt cgagattttc ggaagatgtg aaaattgtca ataaggattt
    43021 aaataaaata acatttgata attttaaaaa aaagcaagat ttccttgacg gtcaaaacag
    43081 cattagatat aatatttaca ggacagtctt gttttttgag atatatttca ataacaatta
    43141 cggttacttt ttaagctcgg agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa
    43201 acgaatcact tgacgatgct cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt
    43261 tacaagaatc tcgtaaacgt gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat
    43321 ctgaagcagc tagaaaacgc aaaaaattct agttctgtga tgaattggag ttggtagtgt
    43381 gtcactacta agtagattaa acgatgacat gaaaacagcg atgaaagcga aagataagga
    43441 atctttacaa gtcatccgaa tgatcaagtc atctatacaa aacgagcaga ttaaagaagg
    43501 tcacgacttg acagaagaag aagaattaac ggtgttgtct cgcgaaatga aacagcgacg
    43561 tgattctttg catgagtttg aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag
    43621 tgaaattgtg atagtcgaaa aatacatgcc ggaacaactt tccgacgaag aaattcgaca
    43681 acttgttcaa gaagcaatca cgcaaactgg tgcttcatca atgaaagaat ttggcaaagt
    43741 catgggtgcg atcatgccta aagtcaaagg aaaagcagat ggaaaccaag taaatgccat
    43801 tgtgaaagaa ctattacaat cttaatttta tcatttaaac tgcggacaat cattgtccgc
    43861 agtttttttg tgctcgtaaa aaagagatct aaaaaatagc tagacgaaag aataggattc
    43921 tttcatctag ctgttttttt cctgtttaat atttcttatg ttaaattgtg tttgatctgg
    43981 ttattcttct ggtagttcat ttgcttcttg tttataccct gataggcgat aggcgtaata
    44041 gaagagtaag ctgccagcat acccaagtaa cgttgggacc atccagccaa gtcccaatgc
    44101 agcaaaagga acgtgatgat gataaaattc gagcatgctt gctacaactc tttgttgact
    44161 cactaaagca ggcgaatttg ccagcatatc caatacggcg ggaacagcag caaacaacat
    44221 cgttacttga tagacaaatg gtgtcttttg gaaaaatttt gctgttaagg aaagcaaaat
    44281 caacgcaagc gacaatggat acaaaagcat caatactggt aaagaccatt ggatgatatt
    44341 gtccaaacca gcatttgcaa cgacaaaaga gacgaaagtc gttaaccgca gccatgtcat
    44401 ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa cgagtcccat
    44461 agcggtagtg aagacaccta atgtgaccat cactcctaag aagctagcgc cgaagttacc
    44521 taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata aagacatctt
    44581 attcaaactc atcgcaccga gaagaacaag accaaaatag atcatgactt ctagcgcaac
    44641 gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttga aaccaaggtc
    44701 acggactgca tggacaaaag tgacacttaa ggcaagcaag gctactgcat caaccgtgtt
    44761 ataaccttct aggacaccat ttgttaaagc gtttgtttgg taagtatctg cagcagattg
    44821 atttagattt cccattgggt ggaataaaga aacgacaaaa atgattgcta ataatgcaag
    44881 aaacaaggag tttagatact ttccgacgta tttgaccaac cgtgtgggat ggacagtaag
    44941 gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg aattatattt
    45001 ttcaggcaaa aatggtttag cagccatttc aaaagcagta gcagctgtcc gtggtgtacc
    45061 gaaaaatggt ccgatcgtca aatgaacaag gattaagaac aaggcagcat accattttcc
    45121 gacaggttta gctaagtcat acagaccatc actttttgtc actactacag ctaagatagc
    45181 tagtaatggg aaaagagcgc ctgagatggc aaatcctacg ccagcactga cccagtttgc
    45241 gccagccatt tgacctaagt ggacaggaaa gattagatta cctgacccga aaaacatccc
    45301 gaagataagt gaactgacca tcaatagttg tttccatgaa taagtttttg tttcttccat
    45361 aaatttttcc tccaataatt atcattttga ttgacgatat aaacgttgca gcaattagta
    45421 atgccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa aaagcccttt
    45481 tccacttggg aaaagggctc tagattctag aacggtacca ccttttttcg cttaaaaaag
    45541 cttcttcatt cacggacgct ttttgataaa gttatccgct agcctgataa tggaggcagc
    45601 cacaatgatc tacgttttcg acattgactc ggaaatgatg ttcgtctatt cgatttattg
    45661 tcatttctca cttacatgac tcactgggaa taaatcgttg aatagcctac tgttttcctc
    45721 gacgctttgc tgtatgttta tttgttgacg taataataca cctgtttttt ttgaaatgca
    45781 agaatatttt aactattttg ttttcttttt ttgaaacaag aaaaatacct agagaatacc
    45841 tctgaatgaa acggaatatt ttgttctcca ctttcatata acttacattc tcagattctt
    45901 tttatcaaaa cgagaccttt agcgaatgga aacagctatt ttttaacgtt tcttggtacg
    45961 tatggttaaa cgactctttt acaacctttt cttatttgct atatagtttc ttctgagtat
    46021 ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc tatttttgac ggaagagtct
    46081 agttctttag agattagatt gacagctgct gatgatgtca gcatgctttt aggtgcacac
    46141 gacaagcata taaaactgat cgaggaaaca acggaaacaa ccatccatac acgtggagaa
    46201 atcattcaga tcattggtga acaatcccag atttccttgg ctgcctcagt cattcggaca
    46261 ttacaggaat tgatcaaacg aggaatccac gtaagtacac cagatgtcgt gacggcattg
    46321 aaaatggggc aaaaaggtac attagattat tttgttgaga tgtatgagga agagatcgtc
    46381 aaagaccgta atggaaaacc tatacgggta aaaaacagcg gtcaaaagaa atacgttgaa
    46441 tcggtagcca aacatgacat cgtatttggt gtagggccag ccggaacagg gaaaacgttt
    46501 cttgccgtag tattagcaat tgctgcccta aaaaaaggcg aagtgcaaaa aatcattttg
    46561 acgcggcccg ctgtagaggc aggggaaaac ctaggctttc tacccgggga tctgaaagaa
    46621 aaagtggatc cttatctgcg gcctgtttat gatgctttat accaaatttt tggattggat
    46681 catacgaacc gattgatgga acgaggcgta atcgaaatcg caccattagc ttacatgcgg
    46741 gggcggacat tggatgatgc attcgttatc ttggacgaag cacaaaacac gacagtcgct
    46801 cagatgaaaa tgttcttgac ccgtctcggt tatcaatcaa aaatgattgt caatggggat
    46861 acaagtcaga tagacttgcc tcgagggacg acaagtggtc ttgttcatgc agaacgaaca
    46921 ctaaagcaaa tcaaaaaaat cgattttgtt aattttgaag caagtgacgt tgttcggcac
    46981 cctgtagttg ccgaaatcat caaagcttat gaaaaggcgg atcttcatca agaataagct
    47041 ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa gaaaaactcg
    47101 gaaaggcagc attgcctttc cttctgttct tgttttttat cattgtctgc ggcgttacat
    47161 ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca gaagagagta
    47221 tccgtgcgaa taaaacgatc gagaacacgg aagaaaccga acaaagaaga aaactggcag
    47281 cagaagcagt gactccagaa tatacttatc aaaaagatct ggcagatgac cagaacaatc
    47341 ggatcaagca attattcgaa ttgatcgata agacgaatga taccatcaac aaatcttata
    47401 atgacaaggt agataaggcg aaggaagatg aaaatatccc gaaacctact acagaagagc
    47461 ggattgctac actgaagaaa aattttgaaa acgtgaatgc agaaaacgtt gcgttttacc
    47521 aaaaactgcc gaacaatttt tattcgacga tatttgaaat gactgaaacg gaaattcaca
    47581 cggttaggga tgaaagtttg aagctcattg acgaacagat gagtaaacaa gttcgtgaaa
    47641 gcgaattgga agcttttaaa caagaagcag aagatcagat tcagtatttg aatgtcacaa
    47701 cagagcagca acaaatgatt cgctacttag tggatcaagg cattgtagtc aatgatgtat
    47761 tgaatgagaa aaagacagaa gaattaaaac aatctgcacg agaagctgta caaccagtga
    47821 tgatcttcca aggagaaatc attgttcgcg aaggaaatca aattgatgct tcagcgatga
    47881 aaaaattgga attactagga ttgacgaatc aaacgacttc gattttccca ctcattgcca
    47941 tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt caattccaaa
    48001 atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca gtcagcatag
    48061 tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc ccgctgtttt
    48121 atcccgctgc ttttgcacca cttgttttaa gctttttctt gaaccgaaga gcagggatca
    48181 tggctggtat tttccaagca gtatctgcac ttttcatttt ttatgattcg attggcacga
    48241 actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca acgattgtca
    48301 gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt atttttcctg
    48361 tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat gcaacgactt
    48421 ggttaatgct tgtctgcggg ttcactggtg cactcttctc attcttgctg acgatcggtc
    48481 tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta aatgaactca
    48541 gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt acttatcatc
    48601 atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc ggacgttctt
    48661 tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat gctagctttt
    48721 tcgttgagaa cttgccttca ggagcggaaa acccgcacaa tttcttattg ccagaagaca
    48781 gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa gattataata
    48841 tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta atgcgttatt
    48901 tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc cgttatcctg
    48961 gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt tgtgaggctg
    49021 cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc cataatctta
    49081 tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg aaagaaatcc
    49141 gaaaagtgga aaaatcattg atcagcggtt taagtagcac cttccattct cggatcaaat
    49201 atcctaaaat gaaaacagaa gttgagaaga taaaggaaga acaagaagaa tagaaagaag
    49261 cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga agagaaaatg
    49321 aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc agaggaaaca
    49381 gaaatgtctg ttactttcat ggacaatgcg gcaatccaag taatcaatcg tgattaccgt
    49441 ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg cgaagacgaa
    49501 atcccagtga tttttgaaga tggagagaat cctttgcctc gtgtactagg agacatcatg
    49561 atttctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata tgatcgggaa
    49621 cttggctttt tagcggtaca tggattcttg catatcaacg ggtatgacca tatgacacca
    49681 gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta tggactcaaa
    49741 agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg ctgtccaagg
    49801 agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt tcggtatcct
    49861 tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat ggctgctttt
    49921 agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa atgtagtgga
    49981 catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata tggcagctgg
    50041 agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat ttgtccctaa
    50101 aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca taaatcaggg
    50161 tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa ccgaatcgtg
    50221 ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa aatccaagga
    50281 gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat ccataaacca
    50341 aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa agaagtggat
    50401 gcagtcttat ttatgatcag tgctgatcaa aaacgcggcc ggggagacga ttttattatt
    50461 gaacgtctaa aaaacgtgca ttctccagtt tatctgatta tcaataaaat cgataagatc
    50521 catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc ttttgcacaa
    50581 gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga tgtattagtc
    50641 agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga tcatcctgaa
    50701 tactttatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg agacgaagtg
    50761 cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga taaaatccat
    50821 atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat cggcaaaggc
    50881 ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa tcttttagga
    50941 gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga caaaaaagtc
    51001 tatctgcaag actttggcta ccgaaaagac gaatactgat attctgaact tcttataggt
    51061 taagtataga aataaactgg ataaaaatcc gcacgttgag aaaaacggcg gaagatcgaa
    51121 acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta ggagggagaa
    51181 tatgagccag cttgcagaat cgaaaggctt gattttatct agccgtaatt acaaagaaaa
    51241 agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt atatcaaagg
    51301 aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg aagcagtcta
    51361 tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag acgtccatgc
    51421 gtttcggaac atccaagaag atatcttttt gagcggttat ggtacctatt tgctaaatct
    51481 agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat ttacgcaaca
    51541 agctctgcag cggatggatc aaggagatga tgcagaaatc atcacgaata tctttgaagt
    51601 tcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg tatgcgggga
    51661 aacaaaagga aaatttgact attcttccaa atctggcggt gtgatatgcg agaaacactg
    51721 gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg ttcgaatgtt
    51781 ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa cgaagcaggc
    51841 aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc taaaaagcaa
    51901 aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag agaaaaatat
    51961 gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa aaaaatcaaa
    52021 actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa tagatcgttc
    52081 actgagagga tctattatat aaacaagaaa actgcaaaat gaatacgtac attgaccaag
    52141 atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac ccaaacgatt
    52201 tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt cggaagtagg
    52261 gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac tttttgtcgt
    52321 tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc aaaacagatg
    52381 agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg gtcatcaaac
    52441 ggctgcatgc tgatgcaagc ttatgacaca gaaaaaggag caggtacgat gagcccatat
    52501 acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga gccatcccgc
    52561 cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca tcatcaattt
    52621 caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga aagcttgcgt
    52681 cttttaggaa ttgatccatt agaacacgat attcgttttg ttgaagacaa ctgggagaac
    52741 ccatcgatgg ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat ggagatcaca
    52801 caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac ttctgagatc
    52861 acttatggac tagaacgtct tgcttcatat attcaagaag ttgaaagtgt ctatgatctg
    52921 gaatgggctg atggtgtgaa atatggcgag atcttcaaac aaccagaata tgaacattcc
    52981 aaatattctt ttgaagtaag tgaccaagac ttgctactaa gcaactttga ccgttttgaa
    53041 aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga ttatattttg
    53101 aaatgcagcc atacattcaa tttattagat gctcgcggtg cagtatccgt aacagaacga
    53161 gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat ctttgtggca
    53221 gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc agaaaacatg
    53281 aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg aagaaatgcc
    53341 tgctcatgtt gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa aatttttaga
    53401 tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc gcttagcaat
    53461 caaagtaaca aacattccag aacgtcaagc agacagcgaa gaagaagtca aaggaccagc
    53521 caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag gatttgtccg
    53581 cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg tggaatatgt
    53641 ctatgtaacg aaatacactc acggcaaaga agcaaaagaa gtattgacag gtttagcaga
    53701 tgtcattaaa agcctcactt tcccagtaac gatgcactgg gcagaatacg attttgaata
    53761 tattcgccct attcactgga tcgtggcatt gttagatgac gaaataatcc cgtttgagat
    53821 cttggatgtc aaaacagaca gagcttcaag aggtcatcgt ttcttaggcg aagatgtgac
    53881 atttacgcat gcaaaagaat acgaagaaaa attgaatgaa caatttgtta ttgcttcacc
    53941 gcaaaaaaga aaagaaatga tcatcacaca agcaaacgaa ttagccgaaa aaaatcactg
    54001 gatcattgat ctagatgaag atttactaga agaagtcaac aatttagtcg aatatccaac
    54061 tgtcttttct ggcgacttcg aagaaaaata tctatctgtt cctgaagaag tactagtcac
    54121 ttctatgaaa gaacatcaac gttatttcga agtacggaac gatcaaggta tgttgcttcc
    54181 tcattttatt tctgctcgta atggcgatcg ggtaaaattg gaaaatgtgg tacgaggcaa
    54241 tcaaaaagta ttgacggctc gtttagaaga tgcggaattc ttttataatg aagacaagaa
    54301 agcttcaatc gaccaatacg ttgaaaaatt aaaaactgtt actttccatg aaaaaatcgg
    54361 tagcatctat gagaaaatgc aacgggtaca agtcatcgct cgcatcatag gcgaacaagt
    54421 aggactatct gaggaagaat tgatggatct gcaacgcgct gctgaaatct ataaatttga
    54481 tctagtaacc aacatggtag gagaattccc tgaactacaa gggatcatgg gagaaaaata
    54541 tgctctgctg aaaggcgaga aaccagccgt agcaacagcg atccgtgaac attacatgcc
    54601 tatatccagt gaaggagatt tacctgagac tacagtaggt tctgttttag cgattgctga
    54661 taaattagat agtatctttt ctttctttgc agttgggatg atcccaagtg gctcaaacga
    54721 tccatatgct ttgcgccgtc aagcatatgg tgtcgttcga atcatcgaat cgaaaaaatg
    54781 ggcctttcct ttgtctgttc tgcaagaaac gattagtgaa gttatcagta aagatacgga
    54841 tcgttttggc attggcttgt cagcgggaca acaacaggtg atcgacttta tcaaaggccg
    54901 tctgcgccaa ttattgacaa cgaagaatat ccgtcatgat gtcatcgaag ctgttttgaa
    54961 tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca agcagcatct
    55021 tgcagatgaa gacttcaaac catcgatgga agctttgaca cgtgtcgtga acttagcgaa
    55081 aaaagcggag cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa acgaagcgga
    55141 gaaagaactg cacaaagcag tagaggcaat gaaaacaagc tttgctgacc aaacgatcaa
    55201 cgaaaattat gatcagcttg taagccttcg cccactgatc gaaaattatt ttgatcagac
    55261 gatggtcatg gcagaagaac cagcagtacg taagaaccga ttaacgcaat taagtcaaat
    55321 tgctgccatg gctttgtcac tagcaagttt agatcaaatc atcacaaaat aatttctaat
    55381 atgtaaataa accagagaac aggcacaaaa t
//
LOCUS       DAYYCQ010000007        52348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000007 DAYYCQ010000000
VERSION     DAYYCQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.007"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..1581)
                     /locus_tag="ACHAYF_000469"
     CDS             complement(7..1581)
                     /locus_tag="ACHAYF_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8577934.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(1836..2345)
                     /gene="mreD"
                     /locus_tag="ACHAYF_000470"
     CDS             complement(1836..2345)
                     /gene="mreD"
                     /locus_tag="ACHAYF_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8577935.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(2349..3203)
                     /gene="mreC"
                     /locus_tag="ACHAYF_000471"
     CDS             complement(2349..3203)
                     /gene="mreC"
                     /locus_tag="ACHAYF_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8577936.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(3358..3996)
                     /locus_tag="ACHAYF_000472"
     CDS             complement(3358..3996)
                     /locus_tag="ACHAYF_000472"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8577937.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3997..4647)
                     /gene="rpe"
                     /locus_tag="ACHAYF_000473"
     CDS             complement(3997..4647)
                     /gene="rpe"
                     /locus_tag="ACHAYF_000473"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8577938.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4660..5559)
                     /gene="rsgA"
                     /locus_tag="ACHAYF_000474"
     CDS             complement(4660..5559)
                     /gene="rsgA"
                     /locus_tag="ACHAYF_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8577939.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5724..7793)
                     /gene="pknB"
                     /locus_tag="ACHAYF_000475"
     CDS             complement(5724..7793)
                     /gene="pknB"
                     /locus_tag="ACHAYF_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8577940.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7790..8530)
                     /locus_tag="ACHAYF_000476"
     CDS             complement(7790..8530)
                     /locus_tag="ACHAYF_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8577941.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(8543..9901)
                     /gene="rsmB"
                     /locus_tag="ACHAYF_000477"
     CDS             complement(8543..9901)
                     /gene="rsmB"
                     /locus_tag="ACHAYF_000477"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8577942.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9901..10839)
                     /gene="fmt"
                     /locus_tag="ACHAYF_000478"
     CDS             complement(9901..10839)
                     /gene="fmt"
                     /locus_tag="ACHAYF_000478"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8577943.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10832..11323)
                     /gene="def"
                     /locus_tag="ACHAYF_000479"
     CDS             complement(10832..11323)
                     /gene="def"
                     /locus_tag="ACHAYF_000479"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8577944.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(11338..13746)
                     /gene="priA"
                     /locus_tag="ACHAYF_000480"
     CDS             complement(11338..13746)
                     /gene="priA"
                     /locus_tag="ACHAYF_000480"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8577945.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13999..14304)
                     /gene="rpoZ"
                     /locus_tag="ACHAYF_000481"
     CDS             complement(13999..14304)
                     /gene="rpoZ"
                     /locus_tag="ACHAYF_000481"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8577946.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(14309..14923)
                     /gene="gmk"
                     /locus_tag="ACHAYF_000482"
     CDS             complement(14309..14923)
                     /gene="gmk"
                     /locus_tag="ACHAYF_000482"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8577947.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(15410..15694)
                     /locus_tag="ACHAYF_000483"
     CDS             complement(15410..15694)
                     /locus_tag="ACHAYF_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577948.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15712..16602)
                     /locus_tag="ACHAYF_000484"
     CDS             complement(15712..16602)
                     /locus_tag="ACHAYF_000484"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8577949.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16622..16840)
                     /locus_tag="ACHAYF_000485"
     CDS             complement(16622..16840)
                     /locus_tag="ACHAYF_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8577950.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16810..18510)
                     /locus_tag="ACHAYF_000486"
     CDS             complement(16810..18510)
                     /locus_tag="ACHAYF_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8577951.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18777..19550
                     /locus_tag="ACHAYF_000487"
     CDS             18777..19550
                     /locus_tag="ACHAYF_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577952.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19631..20200)
                     /gene="thiT"
                     /locus_tag="ACHAYF_000488"
     CDS             complement(19631..20200)
                     /gene="thiT"
                     /locus_tag="ACHAYF_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8577953.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(20266..20368)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20374..21345)
                     /locus_tag="ACHAYF_000489"
     CDS             complement(20374..21345)
                     /locus_tag="ACHAYF_000489"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8577954.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(21439..22812)
                     /gene="glmU"
                     /locus_tag="ACHAYF_000490"
     CDS             complement(21439..22812)
                     /gene="glmU"
                     /locus_tag="ACHAYF_000490"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8577955.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(23315..24133)
                     /gene="purR"
                     /locus_tag="ACHAYF_000491"
     CDS             complement(23315..24133)
                     /gene="purR"
                     /locus_tag="ACHAYF_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8577956.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(24300..25124)
                     /locus_tag="ACHAYF_000492"
     CDS             complement(24300..25124)
                     /locus_tag="ACHAYF_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8577957.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(25097..25777)
                     /locus_tag="ACHAYF_000493"
     CDS             complement(25097..25777)
                     /locus_tag="ACHAYF_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8577958.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25800..26738)
                     /locus_tag="ACHAYF_000494"
     CDS             complement(25800..26738)
                     /locus_tag="ACHAYF_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8577959.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26983..27834)
                     /gene="ispE"
                     /locus_tag="ACHAYF_000495"
     CDS             complement(26983..27834)
                     /gene="ispE"
                     /locus_tag="ACHAYF_000495"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8577960.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27988..30561)
                     /gene="mprF"
                     /locus_tag="ACHAYF_000496"
     CDS             complement(27988..30561)
                     /gene="mprF"
                     /locus_tag="ACHAYF_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8577961.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30702..31508
                     /gene="proC"
                     /locus_tag="ACHAYF_000497"
     CDS             30702..31508
                     /gene="proC"
                     /locus_tag="ACHAYF_000497"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8577962.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(31587..33143)
                     /gene="rny"
                     /locus_tag="ACHAYF_000498"
     CDS             complement(31587..33143)
                     /gene="rny"
                     /locus_tag="ACHAYF_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8577963.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(33554..34603)
                     /gene="recA"
                     /locus_tag="ACHAYF_000499"
     CDS             complement(33554..34603)
                     /gene="recA"
                     /locus_tag="ACHAYF_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8577964.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34712..35953)
                     /locus_tag="ACHAYF_000500"
     CDS             complement(34712..35953)
                     /locus_tag="ACHAYF_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8577965.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(36193..36771)
                     /gene="pgsA"
                     /locus_tag="ACHAYF_000501"
     CDS             complement(36193..36771)
                     /gene="pgsA"
                     /locus_tag="ACHAYF_000501"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8577966.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36980..37903)
                     /locus_tag="ACHAYF_000502"
     CDS             complement(36980..37903)
                     /locus_tag="ACHAYF_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8577967.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38642..39928)
                     /locus_tag="ACHAYF_000503"
     CDS             complement(38642..39928)
                     /locus_tag="ACHAYF_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8577968.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39921..41189)
                     /locus_tag="ACHAYF_000504"
     CDS             complement(39921..41189)
                     /locus_tag="ACHAYF_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8577969.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            41441..41902
                     /gene="mscL"
                     /locus_tag="ACHAYF_000505"
     CDS             41441..41902
                     /gene="mscL"
                     /locus_tag="ACHAYF_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8577970.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41961..42407)
                     /locus_tag="ACHAYF_000506"
     CDS             complement(41961..42407)
                     /locus_tag="ACHAYF_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8577971.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(42500..42745)
                     /locus_tag="ACHAYF_000507"
     CDS             complement(42500..42745)
                     /locus_tag="ACHAYF_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8577972.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42860..43438)
                     /locus_tag="ACHAYF_000508"
     CDS             complement(42860..43438)
                     /locus_tag="ACHAYF_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8577973.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(43515..44045)
                     /locus_tag="ACHAYF_000509"
     CDS             complement(43515..44045)
                     /locus_tag="ACHAYF_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8577974.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(44049..44909)
                     /gene="rlmB"
                     /locus_tag="ACHAYF_000510"
     CDS             complement(44049..44909)
                     /gene="rlmB"
                     /locus_tag="ACHAYF_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8577975.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44902..45297)
                     /locus_tag="ACHAYF_000511"
     CDS             complement(44902..45297)
                     /locus_tag="ACHAYF_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8577976.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(45294..46706)
                     /gene="cysS"
                     /locus_tag="ACHAYF_000512"
     CDS             complement(45294..46706)
                     /gene="cysS"
                     /locus_tag="ACHAYF_000512"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8577977.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46703..47239)
                     /gene="epsC"
                     /locus_tag="ACHAYF_000513"
     CDS             complement(46703..47239)
                     /gene="epsC"
                     /locus_tag="ACHAYF_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8577978.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(47325..47564)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(47561..49021)
                     /gene="gltX"
                     /locus_tag="ACHAYF_000514"
     CDS             complement(47561..49021)
                     /gene="gltX"
                     /locus_tag="ACHAYF_000514"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8577979.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(49191..50348)
                     /locus_tag="ACHAYF_000515"
     CDS             complement(49191..50348)
                     /locus_tag="ACHAYF_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8577980.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(50382..51752)
                     /gene="radA"
                     /locus_tag="ACHAYF_000516"
     CDS             complement(50382..51752)
                     /gene="radA"
                     /locus_tag="ACHAYF_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8577981.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51815..52342)
                     /locus_tag="ACHAYF_000517"
     CDS             complement(51815..52342)
                     /locus_tag="ACHAYF_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8577982.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        14205 a        11400 c         9369 g        17374 t
ORIGIN      
        1 tcattcttac atgctgacag caaagtcagg ggcaaaccat tgaactgaac caacttttac
       61 gctttcgcct ggttgaggag catggatata ttgtcctccg cctaaagcaa tcgctacgtg
      121 gtaagttccg ccttgtgacc cccagaataa caagtctcca gcttttgctt gagaaactga
      181 aatttttgtt ccagctgatt cttgaggaac tgtccaacca ccgatgtcac gaccagttac
      241 ttgcatgtaa acatagcgtg tgaaccctga gcagtcaaag ccacttggat ctttaccgcc
      301 ccaaacatat ggagtaccaa tatatttgta agcttctgct acgattgctg caccattcac
      361 acttccagaa ggagctggag caggtgttac aggttttggt tgttcaggtg ttggtgttgg
      421 cgttggagcc ggagtactac ttcctgtgcc gtttcctgta tcaacacttt gatctgttga
      481 tggtgttgta ggcgcaggtg ttgttgattc ttctgttgtt gaagattcag gcactgttgt
      541 tgattcttct gttgctgaag attcaggcac tgttgttgat tcttctgttg ctgaagattc
      601 aggcgctgtt gttgattctt ctgttgctga agattcaggc gctgttgatt cttctgttga
      661 agattcaggt gttgttgatt cttctgttgc tgaagattgt gtagctgatg atgattcttc
      721 agtcattgaa gattgtgtag ctgatgattc ttctgttgct gagctttgtg cagtagatga
      781 agcttgagta gcttgtgctt cagcctctgc ttgttcgcgt gcttcttttt cagctttttc
      841 ttgtgcagct tgttgacgag cttgttctgc tagacgttgt tgttcacgaa tacgagcttg
      901 ctctgcttca gcttctgctt tttgacggtt caagtcagct ttcttgtctt cagcagttgc
      961 ttgttcagca gctaatgaag ttttcagtac gttcaaatca gcttgtttag ataaaagatc
     1021 gcctttttga gattccaaag cagcttggtt ttcagctaat tcttttagtt tagcatcgtt
     1081 ttcagctttt ttgtcttcta cagctttttt atcttgtttt tgttgttcca tcaagtcgtt
     1141 gtttgctttt accatcgttg tcattgcttg aacgcgtcca atagcatctg ctaatgaatc
     1201 agcattcaat acagcatcga tgtagtttga gcttgtgttg cttacttgtg cttcgcgtgc
     1261 ttgtttttgg attgtatctt cacgtttttc aatacgttct tgtaaatctg caatgtcttt
     1321 tactaattgt gcagattctt gacgcaatgt atcttgtttt gctaataggt cttgtgcttg
     1381 tgtgttgatt tcagaaactt ggctttctaa ggcatcgatt tgtgattgtg catctgcttg
     1441 ttgatttttc aagtctgcaa tcttttgatc ttgctgttgt atctgagaat caaaatcgtc
     1501 cgccgcggca gcgatcggtg atgctacggc agtcaaggtc attgaacata ccattactgc
     1561 tgatattaaa ctctttttca ctcttcattc ctccgactgg cttaatttaa taaatatcta
     1621 aatatattta agtctttctt aacgatataa aaattgtacc atagcagtgt gtcaacgata
     1681 taatagttat gttacaaaaa tgtttcaaaa caacttttaa aaatgacaaa actgttcgtt
     1741 tcagaaaatg atcatgaaaa agccgcgtta agtctagcat ttcttaagat aacaacgatt
     1801 ctttttcgct ttcgacctaa aaataaaaaa agaaattatt catcactgaa taatttctta
     1861 aagggaaaaa tgaataatac aaatataatc atgttcaaca gcagtgtagg tcctaaaaac
     1921 cttgtaataa aatacgtgtt atttactact gctaatttaa aaatcaactg gaccaccatc
     1981 gtaaacaatt cataacccgt gacaaagatg atcatactga aaaattcagt aaagatgttg
     2041 acatggataa cgtctttcat tacatacatc aaccatacaa ttaaagggag agctactgca
     2101 tagataccga taacgccaat atagtaagcg tcatagatag ccccgagaac aatcgtggta
     2161 atcagtaaat aacgtttttc aaacgccata ctgcaacata ataatgccaa tatcagaaag
     2221 tgggcatttg acatataggt gcctttcgac cactccccta gcattcttgt taaatgacca
     2281 tctatcaaca tcaaaaagaa caaaacgatt ggcagatagt acttcattgt ttctttcttc
     2341 aacatggact aatcctccgc tagccgttgg acaatcgtca caactgacac gtcatacatt
     2401 tctgcataag gtttcacata aacttcccga tccagcccat aactatctgg tttcgtttta
     2461 ataaccgtac cgatcggcaa gttagctgga gaattccctc caagaccgga tgtctggaca
     2521 acatcccctt ctttgatatc cgtatcacca gttaattggg tcaccactaa ggcatggagc
     2581 ttttcatcat agtttttaag caaaccaaac gcttcgccat tagccgaaga tacccgtact
     2641 ggaaaatgat tggagctttc attagaggat gacagtaatt cgattttaga cgaagccgta
     2701 ttgacctcga tcactcggcc aatcaatcct ttttgcgaca tgactgccat atttgcttcg
     2761 ataccgtctt tagaaccctt gtctacgatc agcatgtcct gccaactgtc aggcgaacga
     2821 gtaatgacat ttgccgttac tttttcgtaa ctagtcagcg tctgattcaa ctcaagttct
     2881 tttttcaacg cttcgatttc tctctcgtaa ttcttgtttt gctgtgaaac gtcatcatag
     2941 ctatcgattt tttcttttag ccgctcattt tcggcataag ttgaaaaaag attatggaca
     3001 gaatctactc catttcgtac ccaccgtact ggggcagaga ttgttttatc aaccattgca
     3061 acactgtcat taacgatgga ttggaagaaa ttgggctttc cttcattcgc tcgctgcgca
     3121 gcggtcacac ttagtatcgt caccacgatg ataacaataa ccaaggtaat aataatattt
     3181 ttattaggat tgaacttctt cacaaagaca cctcgaatta attagactct tgtgttcaat
     3241 tttatcacta aatgccgtga aacaaaaggt aagttgctcc tcttaagctt ttttttagca
     3301 aataatagat tctgtcattt ttagcctgaa agacaaattt ttttatttgt ttatggatca
     3361 atctttgctt tgaataactg caatcatacc atctgaaaaa gaaaattctg cggtatctga
     3421 aagaaattcg ttacttgcat aagatgtcgg atattctact tctttatttt ccaatacata
     3481 cttgctgcct tttaatgtaa gattcgagac gggagtcaag cagcaataag ctaaatactt
     3541 catatctggt atctgtctta ctatatgctc tccaggcaaa taataagaca aataattttg
     3601 acaatctctg ataataagct gatgggcaaa gggtttgaag cgaggttcca tacctaacca
     3661 gagattggct aaaagatgat ccaatcttcc gcccgtagct ccaatcaaag tgacttccgc
     3721 ttgcgggtaa cgttcaaatg tttttaagat acctaactgt gtatctgtat cgtctttttc
     3781 tgcaggggaa cgggtatact ctttggcttt tgcaaatact tccttctgct cttcgttcga
     3841 caatgaatca aaatcaccca ctgccagatc tactgggtag cctgcttgaa gcaggtacag
     3901 cgcaccacgg tctatcccga cataggcatc gtatttggac agcagagact gcggccagtt
     3961 ttgcggatta cctccagcag ctaataaaat tttcatttag tctaatgcct cttttaatcg
     4021 ttcgatttgt ccgactggat tttcggctcc atagatatat gatcctgcaa cgaagacatc
     4081 cgcccctgca tctttacaga tttttgctgt ttctggaacg attccgccat ctacttcgat
     4141 tgcataagtc caattgttct ctctacgtag atcagcgagt tccgtaattt tttctactgt
     4201 ttcttcaata aagctttgtc cgccgaaccc aggattgaca gtcatgacta gtacttgatc
     4261 agccaaaggc aaaacatgtt tgatcatgga gactggtgtt cctggattga taactactcc
     4321 agctttaact cctaagcttt ttgtcatttg aagtgcccga tgaatatgag gagttgcttc
     4381 gacgtggaca gtaatgatat ctgctccagc ttttgcaaat gctggaatgt agttttctgg
     4441 atcaacgatc atcagatgga cgtctaacgg taatttagta acaggacgga ttgcctgaac
     4501 aatatttggc cctaatgtga tatttgggac aaatcggcca tccattacat ctacgtggat
     4561 gtaatctgcg cctccttttt ccactaactc aatgtctctt tgtaaattgg caaaatctgc
     4621 actcaaaatg gatggtgcta tcttcattct tgtttcctcc tattttcgtt taccttcttt
     4681 ttttgcatac attggttttc ttttttcgat ttcttgtaag aattgcaaat aattgtcgta
     4741 acgtgtctgt gcgataccac ctgtttctac tctatgtttt acttcgcagc caggttcttt
     4801 agcatgcatg cactctctaa atttgcaatg gacagaagct tctacaaatt caggaaattg
     4861 tctcggcagt tctcctgctt caattgtcaa aaagtcaatc gagctaaatc ctggtgtatc
     4921 tgctactaac ccgccataga taggaatcag ttccacatga cgtgttgtat gccgtcctct
     4981 tccaagtgca tcagaaatct ctcctgttgc taaattcagt tccggtgata tttgattcag
     5041 tagcgtcgat tttcctgctc cagactgacc cataaagacc gatagccgtt ctgggaagta
     5101 acggatcagt tctgtcgtat cacccgtatg attgggtaca atcaccggat agcctatttt
     5161 agtataggtt tgcttgatct cttcgatttt ttctgggtcg tctgacaagt cggtctttgt
     5221 caaaaaaata atcggttcga tcgactcgta ttccaaggta actagaaagc ggtcaagtag
     5281 attataagaa aatgcaggct ctacgatact tgtgacaatg attccaagat caacatttgt
     5341 gactggtggg cgaactaatt gattttttct cggcaagatt tccagaagat agccgtctgt
     5401 ttgattttca ctctcaaaga tcacctgatc tccgacaagc ggcgtgattt tccgattccg
     5461 gaaatttcct cttcctcttg tttgataggt ctctccgttt gcgtatacat agtaaaaacc
     5521 gctgattgct tttctgattt gtcctttgat aagtgccacc tccgtttttg tttattttac
     5581 catacgaaga ggaaagtagg tgttattcca tgcaaacttc tcaaagtata tggcttgaaa
     5641 attgatcaaa agagatagaa acacaaaaaa gagaatgcaa caaatcaatg tcgcattccc
     5701 ttctgatata ttttattcag cacttatttt gaactttctg aggtgctagc tgatgaactt
     5761 gtggagctgg aagattcttc ttttgaagag ttgcttgtgc tactggagct acttgagctg
     5821 ctagaactgt ctgaagagtc tttttcagaa ctagaagacg ctggatctgg tcctttagag
     5881 taaattacgt tcacaaccat tcctttggta attgtcgttc ctgcccctgg tactgtacga
     5941 atcactttat ctttatccac cgtgtttgaa tattcggaag tctcatgccc gatatattct
     6001 gcaccgacac tagctaaata actttgtgct tcgctttttg tatagccgga gatatcattc
     6061 aatttaggct ctgttccttg gcttacgaca aaattgatgt aagtttcttc tgctacaact
     6121 tcacccgagg ctggatcctg actgatgatc gaacctgatg gtacagtgtc gctatagtca
     6181 taagtagcac taatattatt gatattgaat ccttgtgtaa caagccaatt acgaacagtt
     6241 tcgtagtttt ctcctacatg actactcaaa gtcactgttt ctggtccttt gctgactgtg
     6301 aaagtaatcg tgtctgtttt aggatcgact tctgtatctt ctgatggaga ttgcgaaatg
     6361 atcattccgc tttctacctc tgaactgaat tcctcttctt tttgaatcaa gtcagcagaa
     6421 aaccctaatt ctttcaaact tgcttcggca tcttcataac ttttaccact gtaatcatct
     6481 aatttgattt ttttcgtgcc gctactaata tacagagtga cttcgcgttt ttgcttgact
     6541 gtcgttcccg ctgccgggtc tgtcttcact acattacctt cttcgatttt gtcatctgca
     6601 atttcttctg tttttgttgc tacttctaat ttcgcttttg cgagtgtttc tctagcagaa
     6661 gcttcagaaa gatcagaaac atcaggaaca acaacttctc ctcgcccgcc agaagcaaag
     6721 aatacagctg ttccgattcc taaaacagca agaatagcta ataggatcag ccaccatttt
     6781 ttctgtcctt tctttttagg atcaggcaga acttcttcct cctctggtaa ggtttcgttt
     6841 tctggcttgt cttttggcag aggcatagat ttgaatgaat caggcattgg tgcatcttcc
     6901 gtaaggggtg tgataacttt tgtttcatta tccatcacat gaggctgcca tttttcttcg
     6961 tttgcacgag ctggtgataa tactgtagaa agatcatcag acatttcctc ggctgactta
     7021 taacggtctg caggttcttt agccgttgcc cgtaaaatca cgttttccag tgcttgtggc
     7081 acatttggat caagtgcttt tatagatgga agatcatctt gaaaatgttt taaagcaatt
     7141 gttacagcag attcaccatc aaacggtacg ctacccgtca acatctcata aaggatgatc
     7201 cctaaagcat aaatatctga ctgcttcgtt gccatacttc ctctggcctg ctcaggggac
     7261 agataatgaa cagatcctag catcgtattc gtctgcgtga tcgatgtttc agataaagcg
     7321 atagcaatcc cgaaatccgt gatttttaca acaccttcat tatcgatcag tacgttttgg
     7381 ggtttcaaat ctcggtggat gatttgatgg ttatgagcta aagaaatagc agaaaggatc
     7441 tgctgcataa tatttactgc tgtttcataa ggaacaggat aatgtgtttg gatatatcgt
     7501 ttcaagtcca ttcctttgac gtattccatc accagatact gcatgttgtc ttcttcacca
     7561 acatcgtaaa cactaacaat gtttggatga acgagttctg ttgctgctaa agcttctctc
     7621 tgaaatcgtc gaatcgcagt ctggtcattt tgaaagtcaa atcgtaagac tttaactgct
     7681 acatcacgat ccaagattaa atcgtgcgcc aaaaatacat ttgccatacc accgctaccg
     7741 atatttccag tgatttgata acggccattt aattttttgc ccaactcgat catacactgt
     7801 cctccttata ttcgataaga agaacggtga tattatccgc tccgcctgct tcattggcac
     7861 gatcaatcag ctcagttact ttatgagaca atgggccttc ttgattgatg atcgttccta
     7921 tttcttcttc agacagcatg ttcgtcaagc catctgaaca taacagcagt cgatctttta
     7981 gttgccaaag atgagtagaa acatctactt cgactgttcc aggcatgccg acagaacgtg
     8041 ttaaaatatt tttccttgga tggttcactg ccatttcttc gctgatttct cctgatttga
     8101 ctagttcatt gactaaagaa tgatcctccg tcaattgcat gattttatct tcacgaatca
     8161 aataagctct gctatcccca acattggcaa tcgtgaacca gtcatctgac agagcggccg
     8221 ctacgattgt tgttcccata ccgttatatt ccggctgttc ttgtccacgc tgataaatca
     8281 gcatattttc ttcttgaatc gattgaatga accattgggc gattttttca tcgtcaacag
     8341 cttcttgttt ttcccaagct tcgcctagat tcgttaccgc catctgactg gcaatgtctc
     8401 cggcacgatg accacccatc ccgtctgcta atacagcaag cttgagacct gaacggctgg
     8461 taaatacact ggcatagtct tgatttgtgt tacgtcgtct tcctacatct gattgatatt
     8521 caatctgcat tttttcacct cattactttt tgcgcaaaca acaaatgaaa aatccatctg
     8581 tgtaaaactg atgcggatac aaagtcaaca ttttctcttc gattgatgat tgtagcatag
     8641 gattggtcaa aacatcaatc ctctcaaaat cagggtgtct ttccaagaaa gcagaaacaa
     8701 cgtcttggtt ttcttctttt aagatcgtac aagtgctata agtcagaata ccggaagact
     8761 ttaaagttgg tgcgcagctt tctaaaattt ccaattggat ttttggtaaa ttcgctaatt
     8821 catccggctg ttttttataa cgaatatctg gttttcgccg aagcagccca agacctgaac
     8881 aaggcgcgtc tactaaaatc cgatcaaact gctctgcttc gaactgttta ctgacttctc
     8941 tagcatccat cttttgagcg tagacagcag aagagacacc tagacgtgct gcattatctt
     9001 caatgagttt gactttgtgt tcgtgtacat ctaaagcgac cacttctcca ccgcatgacg
     9061 gatctaaaaa agaagcaata tgcgtcgtct tacctccggg tgctgcacag gcatctaaga
     9121 catgatgttc aggctcgatt tgcatagacg gcgctacaag catcgaactt tcatcttgga
     9181 tcgtcatgac gccatcgtgg aacaatgagc tagaagctaa atgccctttt ttcgctacta
     9241 taccgtatgg actgacttgg cttttttcag ctgtgatctc ttcttcttgc aaaacagtga
     9301 tggcctgatc tctggaaagt cggcgtgtat ctactcgggc actagcatgg ctaggctgat
     9361 atagcgataa accgagcttt tcagtttctt cgtatccgat ttgatccacc agtcgtttcg
     9421 tcatccatag aggcaggctg attttgacag aaagtctttc gataggatca gcaatgcctt
     9481 gcagatctgg tgctccttga cgctgataat tcctcaagac accatttaca aatttccctg
     9541 tcccaggatt tccttttact tttgcgattt cgaccgcctc gtttaaaata gcatgtgcag
     9601 ggactttatc taaaaattct aattggtata cagaaagata aagaagcatc ttgacccagt
     9661 catccacttt ttttgcttta gcgatcaatg gtgcaacgta aaattccaat agtcgctgat
     9721 ggctaatcgt accatagatc aattctgtaa ataaacccac atctttttga tttagctcgc
     9781 tttgtttgat catctcattc aacaaaagat tcgagtaggc gccacccttg ttcactcgct
     9841 ctaatgtcag taatgccata tatcgtactg attttttcat ttttttaggg atttttttca
     9901 ttatttcacc tgctgaccaa tctgaacttg ctgaccgctt ccgttaagga aatcatgtac
     9961 ggcctgtttt cctttaccag ctggctgtaa ttcattgatg gctaagacgg tctgtttacc
    10021 gcaagcaatc catagatttt ttttgtcttt ccggatgatc gtaccaggtg ctttgtctgt
    10081 agtttctgcc aatacttcaa ccgataataa tttccagcgt atgccctcat aggtcgtgaa
    10141 tgcaattggc caaggacgca ttccgcgtac ttggttgtca atttcttctg cagttttatg
    10201 ccaatcgatt gcttcttgtt cccgtgtgat gtttggcgaa aaagttgctt ctgactcgtc
    10261 ttgaggtact ggtttttgtc catccaaaat attgggcaat gtttctagca atagctttct
    10321 tcctaaaagg ctaagctttt caaacatcgt ccctacatca tcttgtttag tgattggtat
    10381 gctttcttgc gcatagattc cgcctgcatc catttttttg atcatttcca tgatcgttac
    10441 accagtttct ttctccccat tcatgatggc ataatgaaca ggcgcacctc cccgatattt
    10501 aggaagtaaa gaagcatgga cattgattgc ccctaatttt ggtacttgca ataacttttc
    10561 aggcaagaat tggccaaatg ccgcagtgac gatcacatca ggcaccaatt caatgatttc
    10621 ttccatttct ggcgaaccgg agattttctc tggctgcagc acacggatac cgtgcttcag
    10681 agccgctttt tttaccggtg tcggcgtaat tactcgtttc cgcccaactg gacggtcggg
    10741 ctgtgttacc actgcctgga tctcgtaacc tgattctatc agcccttcaa gaatcggcac
    10801 agaaaaagca ggtgtgccca taaatactag cttagtcatc aagatgttcc tccatatatt
    10861 cttcaagttc ttcttccggg atctgctgta tcattttctc aatgaacaag ataccgtcta
    10921 agtgatcgat ttcatgctgg aaggcacggg ctaaatagcc aaaagccgtc acttctatct
    10981 cttctccgtc tcgatcataa taacgcacgg tcacttcatc tgctcgcttg accgttccat
    11041 agacatgcgg aatactgaga caaccttcta cgtcaaggct ttcacctttt gcttcgatga
    11101 tttctgggtt aatcagttca aatttctcgc cttcatctac ttcgatcaca gcaattctct
    11161 tgttttgtcc tacttgagga gcagcgatcc ctattccgtc attcgcaatc attgtctcat
    11221 ataaattgtc taataaagcg attgtctcgt ccgtgattac atcgattggc tgagcagttc
    11281 tttttaattt atcattaggg tggatcaata taggatagcg cataatttgg ccctttctta
    11341 aataaagttt aatggttcgt tgtcaattgc cacaaaaaga ccttgtcgct gttctttctg
    11401 gcttccattc aaaatttcat ccaaaacatg aggaagattc ggttcatgtt tatatttgat
    11461 gattagttga taataatatc ggtttttcac tctcaaaatc atgctgggcg ttggccctaa
    11521 tagcaagctt tgagggctta acacttcttt tagctgattg gcaatctgaa agattttctt
    11581 tgccgctact tgttcttcag gatggctggc ggttattttc acagtaaagt aataaggagg
    11641 atagcctcca cgatgccgta catgcatttc ctgctgataa aactgctcat aattttgttt
    11701 tttagccaat tctatggcgt aatgttgagg attgaatgtt tggatcacga cttcgcctgc
    11761 tttttccgct cgccctgctc gtcctgaaac ttgggtaagc aattggaatg tatgctcact
    11821 agaacgaaaa tccggtaaat tcagggaggt atcagcattc aacaccccaa ctaatgtcac
    11881 ttctggaaag tccaaacctt tagcaatcat ttgtgtccct aataatatat ctgcttcttt
    11941 tgcaccgaat ttttgtaaaa tctgttcgtg tgcccctttt cttcgagtgg tatctacatc
    12001 catacgcaaa atcctagctt gcggataaag ttctcggagt tcctcttcta ctttttgagt
    12061 acctgttcca tagtagcgga ttttattgcc gccacaatta ggacagcgat tgggaatagg
    12121 ttcttcatgt ccgcaataat ggcagcgcat tgaacgtgta tccatatgca gtgtaagaga
    12181 gatatcgcaa ttaggacatg gaagaacaaa tccacagtcc ctacacatca caaaagacga
    12241 atatcctcga cgattgagca gtaggacagt ttgttctttt ttgtctaatc gattttgtat
    12301 tttttcctgc aaattagcag aaaaagtaga ggtccgatga ttctgaaact cttcacgcat
    12361 atctacgatc tcaatagctg gaagctgtgc agctgctttg gctcggtggt ttaactgcaa
    12421 taactgataa acgccttttt gagctcgtgc tcttgattct aaagagggcg tcgcgcttcc
    12481 tagaactatt gggcaatgat gatatttact gcgccaaatt gctagatctc tcgcatgata
    12541 acgcggtgtc tcatcttgtt tgtaacttga ttcatgttct tcatcgataa tgatcagccc
    12601 gatatttttc agcggggcaa aaatagcaga tcgtgcgcct actactactt gcgcttcttc
    12661 ccgctcaatc ttgcgccatt catcatattt ttcaccttgg gaaagtccgc tatgtaagac
    12721 cgcaactgat tctccgaagc gtcctttgaa tcgctcgacc atttgcggag tcaatgcgat
    12781 ttcaggcact aacataatag ccgttttatt ttctgacaaa acatcagcga ttgcctgaag
    12841 atatacttct gttttcccgc tgccagtgat tccctccagc aaaaagacct gatctttttg
    12901 ctgctgacct gctgtgatga tttgttcgac agcatttttt tgttctgcat tcagttccaa
    12961 tgccgtcgtt tggtcaaata ctcggtcttt ataagggtcg cgataacgtt ctgattctat
    13021 aaactccagc cagccctttt cttttccttg attcaagaca gcagtactga ttcctttatt
    13081 tttgaaataa gcaattggtt cttcttggct cagttcagat aaacacaaaa gaagttcttt
    13141 ttgtttcttg gcattttgcc gtaattttgc ccattcttct tcgatttgct tttcagttaa
    13201 caaagaacgg accaaacgaa tcgtttttac tttatttcgt gtatggactt catatttgat
    13261 atctactaat tgctcttggc gccagcgaat caattgagaa aggctgcctt cttcttctgc
    13321 ttccttccag gaaatcactt ctcgctgatc aaacaatacc tgaacttctg gtgtgtctgc
    13381 caaaggatag atcaatttat catattctgc tttcatcaca ctcggaagca tcgtttgtag
    13441 acaagtgatt ttgaatgcgt aagtaatttt tttcatataa tcagctaatg aaagtaattc
    13501 ttcattgacc accggagcaa ggtctagcaa acgaataact gccttcaaag atggattagc
    13561 tgattcttct gatttttgga tagccataac aaatccttgg acatgacgat tgccattacc
    13621 aaaagggact tctactcgca tcccgacttg caaagctgtt tccatttcag agggaaccaa
    13681 ataagtgaat ggctgatcgg tctgcatagt aggtacgtcc acaatgactt cagctgttaa
    13741 tgtcatggtt cgtttcctcc tttttttgtc ttttcttatc aagcgacaaa aagttccttg
    13801 cctattttac tagagatcga tggatttgtc ttatcttctg ccaaatccta acaaaaaatt
    13861 aacagcagat aagaaaaaaa agaactgtgt caaaaagttc agacacagcc ttttctacca
    13921 gacaataagt ggcgaatgtt tgaaaaggaa acagctgggt ttgcttttct ttttagatgt
    13981 tcgctctatt tttctttatt acattttttg ctcttcacgg atacgtgatt ccaattcgtg
    14041 ttgttcacgc tctttttgtg cttgacgttc ctcttcttcc atcttcaaac gttcacgttt
    14101 taattcagga tgtggatcat tgactacatt tcctgcatcg atttcttcta aagcttgacc
    14161 gacgcttttt actgaatcaa aagaatctag agtaggctgt gcacttgcat ccaattcatg
    14221 cgcgcgttta cttgctaaaa tcactagaga atattttgaa tttacacgat ctaatagtga
    14281 gtcaattgaa ggttttaaca tcataagact acatctcctt taacatttta atatatttac
    14341 cgattacgcg atctactcga aaatgttcac tggcaataat gtttttgatt cgttcaactg
    14401 ctagcggcac ttcatcattt acaacagcat agtcgtaaag agccatcatt tcgatttctt
    14461 cttttgcaac tttcattcgt ttttcgatta cttcatctgc atctgttcct cggcctacga
    14521 tccgtgattt taattccgct aaatctggcg gagtcaaaaa gatgaataca ccatcgggca
    14581 ctttttcttt gacttgtttt gctccttgga cttcaatctc caagaaaaca tcttttcctt
    14641 catccaatgt cttgttgaca tatgacaaag gagttccata ataattcccg acatattctg
    14701 cgtattccag catctctcca gcttcgatca ttgattcaaa ctcttctttg cttcggaaga
    14761 aataatcaac gccttcgacc tccccttcac gcattttacg ggtagtcatt gagatcgaat
    14821 actgaaaatc attatcctca ctctcaaaaa ttgcttttcg tactgtgcct tttcctacac
    14881 cggatggacc agacaacacg attaataatc cacgctctga catgacgacg tcctttcaac
    14941 tagtcttttt gtacttatcg tttattttgc actttttttc tgcaactttc aaggttatct
    15001 ttaattatag ctagaaattg gtaaaaaagc taccctgtac caagaggtcg taaaaaagct
    15061 gtcctgcatc aagtaataag aacagccaaa cataaacagc ttctcatgtt tttcgtttgg
    15121 ttggggttat tatcgcagat gcaagctttt gaacgccgtg tataagaggt cgtgaagaag
    15181 ccgattagtt tcgagtatga agaaaaattt tgtgaaaata gtttctcaca tttttgcata
    15241 aatttggctt aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga
    15301 ttgtgacacg aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga
    15361 agaaatccaa ttctgtttat ttattgtatt tctttgttca gcatccttgc taaaagccca
    15421 tattggacaa agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg
    15481 tcggatcttc tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt
    15541 aagcaccgtc gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt
    15601 tccatacgtc aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac
    15661 ttaattcgtg gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat
    15721 attttgtact tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt
    15781 aatttcgatt gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat
    15841 caggaaatct aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg
    15901 tacatggatc gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc
    15961 tagctccgtc aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt
    16021 ttgatacctt atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag
    16081 tacttctttg aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt
    16141 ttctcgactc gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc
    16201 caattcttcc atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcac
    16261 gaagcgttcc gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat
    16321 tccgtctgtc aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt
    16381 ttcgtttttg ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg
    16441 ctgtttaata agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat
    16501 ttgaacatct agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt
    16561 tgtttcgcgg atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc
    16621 attacatatt ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc
    16681 ctttccccat tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac
    16741 cattccagct gactaggttc agttctttac gccacccctt gttgttttct gacaatatag
    16801 cgatttcttc tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata
    16861 atataaatat aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg
    16921 atccttgcgt tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg
    16981 tattttttgc tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg
    17041 tgccaataaa tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat
    17101 gtctacttga tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat
    17161 cgccaatccg catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca
    17221 aaattttatg cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa
    17281 aaatcctcgc cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata
    17341 gtaatagcct aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct
    17401 ttggctatca aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat
    17461 aaaaatgtct ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa
    17521 tgcctctgtt gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc
    17581 gtctgcatat ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga
    17641 ggcgagtatc caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc
    17701 cgctagttct ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag
    17761 taaggacaaa tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc
    17821 ttcaattagt gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc
    17881 ttcaactgca gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa
    17941 attcacagct tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga
    18001 ggtacctgtg ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat
    18061 ctgataagca ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt
    18121 tttatttaat tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc
    18181 tgatagagga gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc
    18241 ttccatatac cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt
    18301 aaaaataaca tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac
    18361 tgctttaaaa gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg
    18421 actttgaaaa aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt
    18481 ctgtgaaaag taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag
    18541 tacgtcataa aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat
    18601 tcataaaacc tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga
    18661 gtgttttcaa tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt
    18721 ttaatgcaaa tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga
    18781 ctgagtcagt ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga
    18841 ctgctactga ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc
    18901 catctgcttc tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag
    18961 aaaatattca gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt
    19021 ggatcgactt tactgattta acgatgctga aagaattgac acctttagaa atttcagaat
    19081 tattatattt tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga
    19141 ataattttgt atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag
    19201 atgaatttta taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg
    19261 taaaaaaatc ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga
    19321 aagaaatgcg taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga
    19381 tcgatggtaa atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag
    19441 gcacccggta tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac
    19501 actggcaatt ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct
    19561 tatcaaaaaa taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct
    19621 tcttttttat ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt
    19681 ggatccctaa taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca
    19741 ttacgaaaga aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca
    19801 ctcctgcaat aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga
    19861 tgctttttct aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca
    19921 tgagccctgc tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga
    19981 cacttaaata ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca
    20041 gcatccctgc ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg
    20101 aaatagaaaa actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg
    20161 caacaatcgt tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga
    20221 ataaaaaagt cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac
    20281 aattcctacg ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca
    20341 gcccaattgg actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg
    20401 actaactggt ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact
    20461 tacttcatcg attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac
    20521 tagacgctcg atagctgaat cttctattct ttgtaaagca gggccggata atactggatg
    20581 tgtacatgaa gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt
    20641 gatcgtacct gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg
    20701 tccaatgata ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc
    20761 aatcggagat ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg
    20821 tgaaacaacg acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag
    20881 tggcgcaccc atcaaatgat cgactggaat atcaaagaaa ccttggattt ggactgcatg
    20941 aaggtccaaa gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt
    21001 cgctgtgatt ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg
    21061 catgactacg ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa
    21121 ctccatcaag ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc
    21181 acgaatactt tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga
    21241 ttttcctaac tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt
    21301 taacgcaaaa atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt
    21361 ctttttcaca actctagaat tctacctaat aaatagtagt catatttttt aaataaatca
    21421 agtgattttg caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt
    21481 gacttgtctt gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga
    21541 cccggcagca atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga
    21601 accaataaag ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac
    21661 gaagacaaca ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt
    21721 caagtgaccg actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc
    21781 gatatggacg ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc
    21841 tgcatgtttc ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc
    21901 aatcctactg tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt
    21961 atttccttgg atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc
    22021 atcgatgtat gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt
    22081 aattcgcttt ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat
    22141 tgattcttca aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat
    22201 ttcgatgata tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa
    22261 cgcatcgaac aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag
    22321 agcttcttct ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc
    22381 acggatgatt cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact
    22441 tgcatttttt ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg
    22501 tgtatcgcca ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg
    22561 caaaacagca tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc
    22621 tagttgtgct ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt
    22681 cgtttcactg acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa
    22741 aactttatat aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata
    22801 acgtgcgtcc aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat
    22861 acccattttt tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa
    22921 atagatagac cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg
    22981 gtcacaagtc acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga
    23041 cttagagtta aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct
    23101 tcggtattag ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa
    23161 tactcaggac aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt
    23221 ctcggtatta agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt
    23281 actactcgaa actaagcggc tttttcacaa cctcttattc tgaaaaataa tttcctggca
    23341 caaccgtaat atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat
    23401 catcgatggc gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt
    23461 ctgattcgaa ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga
    23521 aatcatccac tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct
    23581 tttcaattct ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta
    23641 tttttgagtc ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg
    23701 caataggtac gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt
    23761 tagaagcaat gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga
    23821 gataaacata tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt
    23881 caatatattt tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag
    23941 cacccgggat tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc
    24001 ccaaatcttc actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg
    24061 aaatcaattc atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc
    24121 gacgtacttt cacctattcc aactcctttt aagaaatttt atcgttttac attatataag
    24181 tttttcttca aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta
    24241 aaataaaaag aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt
    24301 tagttatttt ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac
    24361 aaagcaataa agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta
    24421 gttagtcctg acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg
    24481 aagcttttgc ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa
    24541 agtgcaccgg caatcgggat catcacagca atagccacac cagtaatcac attgaacatc
    24601 atcgacatca aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga
    24661 acatacatcg gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct
    24721 aaaaagatga cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata
    24781 cttgttgctg aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca
    24841 gccattaaaa tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc
    24901 aatatcacag ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa
    24961 aagaatccta acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat
    25021 tgtcttctga tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg
    25081 gcaagaaacg ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc
    25141 tcaccagtcg gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca
    25201 ttagaactga tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc
    25261 tggattcttc atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag
    25321 caaaaatacg cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc
    25381 gatttcgcat atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt
    25441 tttcagagag acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga
    25501 tcaccgtact aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac
    25561 ttaatttttt tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca
    25621 gtaatcctaa cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa
    25681 tgacaaattc acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat
    25741 agtaaaaggt caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact
    25801 taattgatac tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg
    25861 cctgctttga tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact
    25921 cctgtttcat tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc
    25981 actttgatgt tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt
    26041 tcctgatctg gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa
    26101 taagcaaaag cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg
    26161 gagtattttt gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa
    26221 gtttcctttt tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt
    26281 atagctaata ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct
    26341 tcttctgatt cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct
    26401 gcttcgatca tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat
    26461 tccatgtctg aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt
    26521 gcacttggtt cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct
    26581 tcatccccta cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc
    26641 aaatcctctt ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca
    26701 cctatcatta ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac
    26761 aaatcggaat tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc
    26821 aatagaaatc catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg
    26881 atgagttgct gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt
    26941 aggattcctc agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac
    27001 taaataaact tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata
    27061 atgttgacac aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc
    27121 gtacttgatc atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc
    27181 ttctaaactg tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc
    27241 agcaagtccg tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt
    27301 acgtgtagag acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg
    27361 taatacttgg atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta
    27421 aggaacatct gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag
    27481 attccataat tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa
    27541 tccagctgct actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta
    27601 tttatttttt agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa
    27661 tgctttattt gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc
    27721 tgccaagtct acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata
    27781 taatacatcc agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc
    27841 acccattcga aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct
    27901 tatgtctgcc tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt
    27961 cataatccaa acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact
    28021 cggaacattt cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa
    28081 attacttcct ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt
    28141 cttttcataa gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat
    28201 gactttttct tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc
    28261 tagattggct tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata
    28321 gtcagctaat tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt
    28381 cttcgtcatc ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc
    28441 gacaccgcta gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg
    28501 tgcaccttga agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag
    28561 ttgatcgtac caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg
    28621 actcagccga gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt
    28681 gaagtgaaaa cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc
    28741 aagctgatag cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat
    28801 tttaggattg ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc
    28861 aaaaacgact tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg
    28921 aagctgatgg cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt
    28981 ttcgtactca ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa
    29041 agctagcaat gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa
    29101 caaaataaaa tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc
    29161 aattgcgtaa acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca
    29221 agaataaacg aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat
    29281 caagacatat accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc
    29341 aaaagatagt aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct
    29401 agcaaggccg agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa
    29461 tgagaacggc aataatactt gaaatagccg actgattgca gacagattag tgattgttga
    29521 tagaagtacc atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac
    29581 cttttggaaa aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg
    29641 tgtgatgaac aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata
    29701 taatagccaa actactgcta catcttgccg aacagacaat tgactcattc ctaagatcat
    29761 caaaacgtca aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc
    29821 aatcatgaac ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat
    29881 gatcaaaaat accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag
    29941 caagcctttt ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa
    30001 aatgactggt gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact
    30061 ttctgactga ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt
    30121 cagcataaat agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt
    30181 tccataaaaa ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt
    30241 gatcgtgttt gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc
    30301 ttgtttttct aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag
    30361 cagtccagcc agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca
    30421 aatatcttgc caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa
    30481 cacggagccg aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat
    30541 caagtatcgt ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc
    30601 ccaatgttat cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga
    30661 tagaaaaaga aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg
    30721 gcacagggaa tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga
    30781 aagaagaggt gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa
    30841 ctggcgtcca tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt
    30901 tagccgtgaa accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga
    30961 aagtcttatt catttctatt gcttcaggaa aatcattaat agatttagaa acagcgcttg
    31021 aagatcctca ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag
    31081 gcgtttctgc gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga
    31141 tgatttttga agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca
    31201 ttggccttgc tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga
    31261 ctggtgtact gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta
    31321 tgggttcttg tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag
    31381 tttcttctcc tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca
    31441 tcccagcaat catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata
    31501 aaaaataatg atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg
    31561 ccgcggctct tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga
    31621 ataacgatta ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata
    31681 tcacgtacta aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga
    31741 acttcgcgtc cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata
    31801 ttttccagac ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt
    31861 cttgctgctg agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc
    31921 acatctccat gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca
    31981 gctaattccg caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca
    32041 atatcatgga gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct
    32101 aagataccag ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta
    32161 gttctgaatc gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg
    32221 acttcaaacg ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat
    32281 ttctcgacca tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc
    32341 aaggtcatcc gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc
    32401 ggtgtatcat cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga
    32461 ccttctcgtc caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca
    32521 gtcgtttctg aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc
    32581 gctttgcgat ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac
    32641 tcatgattca gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat
    32701 aaaccagcaa ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt
    32761 tctcgctctt caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag
    32821 ttctcgcgtt tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa
    32881 cgattctctt gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa
    32941 cggtatttct gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct
    33001 tctttttttg cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc
    33061 tcgtgacgtg actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata
    33121 gcgaggagaa tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca
    33181 tctatacagc ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg
    33241 tgtacaaaaa acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa
    33301 taaacaaaga aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac
    33361 tagtttcata taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag
    33421 atgaacagat atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt
    33481 tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg
    33541 tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg
    33601 ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca
    33661 tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat
    33721 cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt
    33781 ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt
    33841 tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat
    33901 ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt
    33961 tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac
    34021 ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac
    34081 gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt
    34141 cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc
    34201 cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa
    34261 gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct
    34321 cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg
    34381 caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt
    34441 aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt
    34501 gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt
    34561 ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta
    34621 tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag
    34681 caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa
    34741 gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat
    34801 atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt
    34861 gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg
    34921 ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt
    34981 ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa
    35041 cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg
    35101 cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca
    35161 ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt
    35221 cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt
    35281 tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga
    35341 tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa
    35401 agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac
    35461 gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg
    35521 catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa
    35581 ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg
    35641 tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga
    35701 ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata
    35761 agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat
    35821 tatctcctac aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca
    35881 agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta
    35941 tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt
    36001 tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat
    36061 aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa
    36121 gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc
    36181 actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac
    36241 acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc
    36301 gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc
    36361 cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa
    36421 acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc
    36481 ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg
    36541 gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa
    36601 ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag
    36661 tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa
    36721 aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac
    36781 tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt
    36841 ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag
    36901 aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg
    36961 cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac
    37021 tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat
    37081 tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct
    37141 gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta
    37201 accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat
    37261 ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac
    37321 ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg
    37381 aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg
    37441 gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa
    37501 ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct
    37561 gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc
    37621 ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag
    37681 cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat
    37741 catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat
    37801 ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag
    37861 ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa
    37921 gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac
    37981 attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg
    38041 ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta
    38101 aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga
    38161 tatttttccg tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct
    38221 taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa
    38281 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
    38341 ttggtacacg gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt
    38401 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
    38461 ctctttgatt acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt
    38521 tgttttattt tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg
    38581 gaggaggttc ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg
    38641 tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag
    38701 ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc
    38761 gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt
    38821 ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg
    38881 gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt
    38941 ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa
    39001 agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta
    39061 aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt
    39121 gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt
    39181 ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt
    39241 gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag
    39301 gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc
    39361 acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga
    39421 tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag
    39481 ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt
    39541 cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct
    39601 gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag
    39661 ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt
    39721 ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa
    39781 attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat
    39841 ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat
    39901 tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc
    39961 tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt
    40021 ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct
    40081 tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt
    40141 gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga
    40201 atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat
    40261 cctcggaacg tatccacgct actagaggca taataagcta gactttcttt ctctctgaca
    40321 ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca
    40381 aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga
    40441 acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac
    40501 agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct
    40561 ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct
    40621 tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac
    40681 cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca
    40741 aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag
    40801 tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt
    40861 aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa
    40921 ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt
    40981 gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccgaata attcaaacta
    41041 tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc
    41101 gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga
    41161 ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc
    41221 cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat
    41281 aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat
    41341 gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt
    41401 tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga
    41461 gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt
    41521 tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt
    41581 cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc
    41641 gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat
    41701 cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc
    41761 caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat
    41821 acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa
    41881 tctttccgat acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc
    41941 ggctttcttt ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga
    42001 atatcatctt ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct
    42061 tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg
    42121 ttaatcgttt cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca
    42181 aggacacgtc caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc
    42241 ttcccatcag tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct
    42301 tgataaggac actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa
    42361 aactctgctg ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc
    42421 cctgcttttt tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa
    42481 tctcaggctc gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag
    42541 taagtacatc tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat
    42601 caacaacgaa tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct
    42661 ttcttcctgt ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct
    42721 tgattgaagc taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt
    42781 tttaaaattt tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc
    42841 tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga
    42901 gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca
    42961 tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa
    43021 actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt gctaaaggtt
    43081 cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg
    43141 agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga
    43201 tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg cttttttcgt
    43261 aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat
    43321 aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca
    43381 gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa
    43441 aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg
    43501 attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt
    43561 tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta
    43621 ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa
    43681 gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact
    43741 gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc
    43801 gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg
    43861 cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa
    43921 tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc
    43981 tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt
    44041 ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca
    44101 acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt
    44161 tcttttttca atcctggact cattcctttg ccttcattac caatgactaa agcaattgat
    44221 cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat
    44281 cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca
    44341 tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt
    44401 tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa
    44461 ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt
    44521 gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact
    44581 ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact
    44641 ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct
    44701 tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc
    44761 ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt
    44821 ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat
    44881 tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg
    44941 gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc
    45001 gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt
    45061 agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag
    45121 catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg
    45181 cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta
    45241 aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct
    45301 tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac
    45361 aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct
    45421 tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag
    45481 atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc
    45541 ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt
    45601 gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa
    45661 gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa
    45721 ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca
    45781 ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga
    45841 tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt
    45901 tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc
    45961 cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc
    46021 ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca
    46081 atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt
    46141 gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta
    46201 cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt
    46261 tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata gcctttttca
    46321 accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt
    46381 gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca
    46441 gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca
    46501 tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt
    46561 gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt
    46621 ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg
    46681 gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa
    46741 cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg
    46801 cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg
    46861 cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc
    46921 cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa
    46981 tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga
    47041 tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc
    47101 ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg
    47161 tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg
    47221 ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca
    47281 tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc
    47341 gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac
    47401 gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga
    47461 ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc
    47521 ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta
    47581 aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat
    47641 gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag
    47701 tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact acttccattt
    47761 gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt
    47821 cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga
    47881 tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga
    47941 ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca
    48001 tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt
    48061 ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca
    48121 attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa
    48181 agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga
    48241 ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat
    48301 gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat
    48361 tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg
    48421 caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga
    48481 tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat
    48541 tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt
    48601 cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt
    48661 gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc
    48721 tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag
    48781 ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt
    48841 tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac
    48901 gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg
    48961 catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca
    49021 ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg
    49081 cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa
    49141 tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt ttattttgat
    49201 tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt
    49261 ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata
    49321 cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag
    49381 gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca
    49441 gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt
    49501 tggaattcag agactttatt caagttatag tcattggtta caatgacacc atctaaaagc
    49561 ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac
    49621 atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga
    49681 ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca
    49741 aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga
    49801 ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt
    49861 cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt
    49921 gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag
    49981 cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg
    50041 taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg
    50101 aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct
    50161 gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg
    50221 ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag
    50281 ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt
    50341 ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc
    50401 aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta
    50461 cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact
    50521 cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa
    50581 cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct
    50641 aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga
    50701 ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg
    50761 aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct
    50821 tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt
    50881 gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca
    50941 tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct
    51001 cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata
    51061 tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa
    51121 atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc tcttacttgg
    51181 ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg
    51241 ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca
    51301 gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc
    51361 tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga
    51421 gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata
    51481 gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc
    51541 cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc
    51601 aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc
    51661 cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg
    51721 caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg
    51781 agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc
    51841 cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt
    51901 ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga
    51961 acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc
    52021 cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa
    52081 attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt
    52141 tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc
    52201 agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag
    52261 cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa
    52321 cttcaaatcc tctttttctc accggttt
//
LOCUS       DAYYCQ010000008        52257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000008 DAYYCQ010000000
VERSION     DAYYCQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.008"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..634)
                     /locus_tag="ACHAYF_000518"
     CDS             complement(<1..634)
                     /locus_tag="ACHAYF_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8577983.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLD"
     gene            complement(813..1532)
                     /locus_tag="ACHAYF_000519"
     CDS             complement(813..1532)
                     /locus_tag="ACHAYF_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8577984.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1663..2814)
                     /locus_tag="ACHAYF_000520"
     CDS             complement(1663..2814)
                     /locus_tag="ACHAYF_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8577985.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3031..3771)
                     /locus_tag="ACHAYF_000521"
     CDS             complement(3031..3771)
                     /locus_tag="ACHAYF_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8577986.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3775..4098)
                     /gene="rsfS"
                     /locus_tag="ACHAYF_000522"
     CDS             complement(3775..4098)
                     /gene="rsfS"
                     /locus_tag="ACHAYF_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8577987.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4155..4748)
                     /gene="yqeK"
                     /locus_tag="ACHAYF_000523"
     CDS             complement(4155..4748)
                     /gene="yqeK"
                     /locus_tag="ACHAYF_000523"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8577988.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4732..5382)
                     /locus_tag="ACHAYF_000524"
     CDS             complement(4732..5382)
                     /locus_tag="ACHAYF_000524"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8577989.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5392..5703)
                     /gene="yhbY"
                     /locus_tag="ACHAYF_000525"
     CDS             complement(5392..5703)
                     /gene="yhbY"
                     /locus_tag="ACHAYF_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8577990.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5719..6828)
                     /gene="yqeH"
                     /locus_tag="ACHAYF_000526"
     CDS             complement(5719..6828)
                     /gene="yqeH"
                     /locus_tag="ACHAYF_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8577991.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6825..7352)
                     /locus_tag="ACHAYF_000527"
     CDS             complement(6825..7352)
                     /locus_tag="ACHAYF_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8577992.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7532..8317)
                     /locus_tag="ACHAYF_000528"
     CDS             complement(7532..8317)
                     /locus_tag="ACHAYF_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8577993.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8310..9179)
                     /gene="accD"
                     /locus_tag="ACHAYF_000529"
     CDS             complement(8310..9179)
                     /gene="accD"
                     /locus_tag="ACHAYF_000529"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8577994.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9180..10559)
                     /locus_tag="ACHAYF_000530"
     CDS             complement(9180..10559)
                     /locus_tag="ACHAYF_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8577995.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10567..10986)
                     /gene="fabZ"
                     /locus_tag="ACHAYF_000531"
     CDS             complement(10567..10986)
                     /gene="fabZ"
                     /locus_tag="ACHAYF_000531"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8577996.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11011..11487)
                     /gene="accB"
                     /locus_tag="ACHAYF_000532"
     CDS             complement(11011..11487)
                     /gene="accB"
                     /locus_tag="ACHAYF_000532"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8577997.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11490..12725)
                     /gene="fabF"
                     /locus_tag="ACHAYF_000533"
     CDS             complement(11490..12725)
                     /gene="fabF"
                     /locus_tag="ACHAYF_000533"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8577998.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12744..13481)
                     /gene="fabG"
                     /locus_tag="ACHAYF_000534"
     CDS             complement(12744..13481)
                     /gene="fabG"
                     /locus_tag="ACHAYF_000534"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8577999.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13493..14410)
                     /gene="fabD"
                     /locus_tag="ACHAYF_000535"
     CDS             complement(13493..14410)
                     /gene="fabD"
                     /locus_tag="ACHAYF_000535"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8578000.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14517..14741)
                     /locus_tag="ACHAYF_000536"
     CDS             complement(14517..14741)
                     /locus_tag="ACHAYF_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8578001.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14794..15756)
                     /locus_tag="ACHAYF_000537"
     CDS             complement(14794..15756)
                     /locus_tag="ACHAYF_000537"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8578002.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15766..16206)
                     /locus_tag="ACHAYF_000538"
     CDS             complement(15766..16206)
                     /locus_tag="ACHAYF_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8578003.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16512..17477
                     /locus_tag="ACHAYF_000539"
     CDS             16512..17477
                     /locus_tag="ACHAYF_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8578004.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(17534..17764)
                     /locus_tag="ACHAYF_000540"
     CDS             complement(17534..17764)
                     /locus_tag="ACHAYF_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8578005.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17850..18737)
                     /locus_tag="ACHAYF_000541"
     CDS             complement(17850..18737)
                     /locus_tag="ACHAYF_000541"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8578006.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18826..20049)
                     /locus_tag="ACHAYF_000542"
     CDS             complement(18826..20049)
                     /locus_tag="ACHAYF_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8578007.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(20066..21112)
                     /locus_tag="ACHAYF_000543"
     CDS             complement(20066..21112)
                     /locus_tag="ACHAYF_000543"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8578008.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(21276..23003)
                     /gene="ptsP"
                     /locus_tag="ACHAYF_000544"
     CDS             complement(21276..23003)
                     /gene="ptsP"
                     /locus_tag="ACHAYF_000544"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8578009.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(23003..23269)
                     /locus_tag="ACHAYF_000545"
     CDS             complement(23003..23269)
                     /locus_tag="ACHAYF_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8578010.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23748..25982
                     /locus_tag="ACHAYF_000546"
     CDS             23748..25982
                     /locus_tag="ACHAYF_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8578011.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            26130..26450
                     /locus_tag="ACHAYF_000547"
     CDS             26130..26450
                     /locus_tag="ACHAYF_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8578012.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(26655..26879)
                     /locus_tag="ACHAYF_000548"
     CDS             complement(26655..26879)
                     /locus_tag="ACHAYF_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8578013.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(27077..28657)
                     /locus_tag="ACHAYF_000549"
     CDS             complement(27077..28657)
                     /locus_tag="ACHAYF_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8578014.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(29058..30434)
                     /locus_tag="ACHAYF_000550"
     CDS             complement(29058..30434)
                     /locus_tag="ACHAYF_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8578015.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(30562..31650)
                     /locus_tag="ACHAYF_000551"
     CDS             complement(30562..31650)
                     /locus_tag="ACHAYF_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8578016.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            31822..32256
                     /locus_tag="ACHAYF_000552"
     CDS             31822..32256
                     /locus_tag="ACHAYF_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8578017.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(32334..32867)
                     /locus_tag="ACHAYF_000553"
     CDS             complement(32334..32867)
                     /locus_tag="ACHAYF_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8578018.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(32961..34139)
                     /gene="mutY"
                     /locus_tag="ACHAYF_000554"
     CDS             complement(32961..34139)
                     /gene="mutY"
                     /locus_tag="ACHAYF_000554"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8578019.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(34132..34929)
                     /gene="recX"
                     /locus_tag="ACHAYF_000555"
     CDS             complement(34132..34929)
                     /gene="recX"
                     /locus_tag="ACHAYF_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8578020.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            35036..36418
                     /gene="rlmD"
                     /locus_tag="ACHAYF_000556"
     CDS             35036..36418
                     /gene="rlmD"
                     /locus_tag="ACHAYF_000556"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8578021.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(36592..37170)
                     /locus_tag="ACHAYF_000557"
     CDS             complement(36592..37170)
                     /locus_tag="ACHAYF_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8578022.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            37492..37962
                     /locus_tag="ACHAYF_000558"
     CDS             37492..37962
                     /locus_tag="ACHAYF_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8578023.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(38070..39296)
                     /locus_tag="ACHAYF_000559"
     CDS             complement(38070..39296)
                     /locus_tag="ACHAYF_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8578024.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(40050..40250)
                     /locus_tag="ACHAYF_000560"
     CDS             complement(40050..40250)
                     /locus_tag="ACHAYF_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8578025.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(40384..40992)
                     /locus_tag="ACHAYF_000561"
     CDS             complement(40384..40992)
                     /locus_tag="ACHAYF_000561"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8578026.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(41077..41547)
                     /locus_tag="ACHAYF_000562"
     CDS             complement(41077..41547)
                     /locus_tag="ACHAYF_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8578027.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(41595..42509)
                     /locus_tag="ACHAYF_000563"
     CDS             complement(41595..42509)
                     /locus_tag="ACHAYF_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8578028.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            42655..43458
                     /locus_tag="ACHAYF_000564"
     CDS             42655..43458
                     /locus_tag="ACHAYF_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8578029.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            43636..44583
                     /locus_tag="ACHAYF_000565"
     CDS             43636..44583
                     /locus_tag="ACHAYF_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8578030.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(44651..44944)
                     /locus_tag="ACHAYF_000566"
     CDS             complement(44651..44944)
                     /locus_tag="ACHAYF_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8578031.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(44972..45637)
                     /locus_tag="ACHAYF_000567"
     CDS             complement(44972..45637)
                     /locus_tag="ACHAYF_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8578032.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(45777..46409)
                     /locus_tag="ACHAYF_000568"
     CDS             complement(45777..46409)
                     /locus_tag="ACHAYF_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8578033.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(46416..47483)
                     /gene="liaS"
                     /locus_tag="ACHAYF_000569"
     CDS             complement(46416..47483)
                     /gene="liaS"
                     /locus_tag="ACHAYF_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8578034.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(47480..48211)
                     /gene="liaF"
                     /locus_tag="ACHAYF_000570"
     CDS             complement(47480..48211)
                     /gene="liaF"
                     /locus_tag="ACHAYF_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8578035.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(48380..48859)
                     /gene="greA"
                     /locus_tag="ACHAYF_000571"
     CDS             complement(48380..48859)
                     /gene="greA"
                     /locus_tag="ACHAYF_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8578036.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(48995..50170)
                     /gene="mltG"
                     /locus_tag="ACHAYF_000572"
     CDS             complement(48995..50170)
                     /gene="mltG"
                     /locus_tag="ACHAYF_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8578037.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            50374..51543
                     /locus_tag="ACHAYF_000573"
     CDS             50374..51543
                     /locus_tag="ACHAYF_000573"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8578038.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(51810..>52257)
                     /locus_tag="ACHAYF_000574"
     CDS             complement(51810..>52257)
                     /locus_tag="ACHAYF_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578039.1"
                     /translation="NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDG
                     QVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVK
                     SESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        15149 a        10933 c         8709 g        17466 t
ORIGIN      
        1 aatccaaatc tgtttcagga tattttaatt tgacaagcga aataatcatt ttcagttctt
       61 ccatgttttc tggatattct gggtcaaata ccttgctatg acggccaatc ccgccattgt
      121 cgatcaatcg gtaaattcga ttccattcaa tgaattggtc aattgattgg aaattgctga
      181 ttggatagcc gccacaatcc aaaatctctg atacacgaaa tgctaggtat gcactatcga
      241 tcactttatt ttttttcgtg tctatcttct catttctttc gataagaatt ttttcaggac
      301 gatgtttcaa gctccactct aatagtagaa agaaagcctc catccgatac tcttcttcaa
      361 ataaccgaaa ggctgaatca gaacgacgaa aaagcagcgg aatcagataa ttttgtctat
      421 aaatcagcat tggtgcgcgg tccaccatac actctaaata acggttcaga taccctgtac
      481 cgacatagtt tcctaatcga tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt
      541 ttgaacaatc agttacgttc acagctaact cttctacttt gcggtaaccg cttaagatac
      601 gttcaaaagg ttctaaagtg atttttgctt ccatattctt ccccgtccat cttccacata
      661 cttcatatca tttcacatga tacttatgct ctcatcatac cacaggttga attagaaaag
      721 aaaataaatc acacaaaaaa gcaagacaat ctgttagtga gattgtcttg cttttcgaaa
      781 aagactgaat acttttcgtt aaaactggtt gcttagtctt ctaatgccac attatggaac
      841 acatgctgta catcttctag atcttccagt gcatcaatca ttttttcaaa tgtttcttga
      901 tcttctggag atagtgttac ctcgttttga gggatcattt ggatctcagc gattgaaaaa
      961 tcagtgattc ctttttcttt taatgcttct tgtatactat ggaaatccgc tacttcaccg
     1021 taaacgatga tttgtccttc ttcttccatt acatcgcgaa catcgatgtc tttttccatt
     1081 aggtattcaa ggatctcgtc agcgtcttct cctgcaaaag caaagattgc tgtattgtca
     1141 aacatgtaag aaaccgctcc ggaaacaccc atgttcccgc catttttccc aaatgctgcc
     1201 cgtacgtctg ctgctgttcg attcacattg ttcgtcaatg tatcaacaat caccattgag
     1261 ccgcttggtc caaatccttc atagcgcaat tcagagtatg tttcatctcc tgcacctttt
     1321 gctttttcga ttgctcggtc aataatatgt tttggtacat tataagtttt agcgcgatca
     1381 atgacgaaac gtagtttttg gtttgcatgc ggatcaggat cacctgattt tgctgccgcg
     1441 tagatttcaa tcccaaattt tgcataaatc cggctattat tagcatcttt agcagttttc
     1501 tttgctacga tatttgccca tttacgtccc atattttcac ttcgctttct gatttgatag
     1561 tttcataacg caaaaacgtc ctgttccatt atacttgtga atcacgattc tgtgaagctt
     1621 tttttctttc ttccttcatt cttctcattt ttagtcaatt atctatactc ggattgggat
     1681 ttgcccgaaa ttctgttccg gaatattgcc tcccaattga tagatttgat cacttctaac
     1741 taacagttcc gcttcttgtg cctctttttt tccgattcgg gaataaatag aaatctcttt
     1801 tttttctttt aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa gataattttt
     1861 attttgttgg acaggaatct cttgcttttt gatattcagc aagacataac aagataatcg
     1921 ttggatccgt gtccatgtat atctttttgt ttttaccttt gacacaaaat cctcaaaact
     1981 atcagcagtt gcagctgctg ttttcatgcg cgattctatt ccttcggtca tctgatagat
     2041 atctgtcagt tccagcaaag tagaagataa caaccgataa cgcaaatacg gccaataaga
     2101 ttcccaagtc tgcagcgaac aagttgaaag atcttcttct gtctgaggcg gcacaagtcc
     2161 agctattctt tttccttctt ctaacgcatg gcgaatggct gttgcactgg ctatcctctg
     2221 atctgtgatg attttttcat gatagcctgc acttttacgt cggatcggat gtaattccat
     2281 agggtggaca tacttggcat tttcctttgc ataacttaat cccaagatat gatttggcga
     2341 agaaaaatca agcgtaatat cgggatacag actgcgaaaa acctcagtca ttttttgcgg
     2401 ataactatac gtttgatcag acagttcttg aaatgcctct tctatcctat cttgattctc
     2461 ttggacaaag cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca
     2521 caaactgtca cagtccagtg tttgcaagat ttttactgca cctttagcaa aatagtcagc
     2581 tgactgaacc gcccattcga cgggaagctc cactactaaa tccacaccat tttgtaaagc
     2641 ttcattcgcg cgatgccatt tatcgatgat agccggttcg cctctttgca aaaaattccc
     2701 gctcattaca gcaatgacga cctctgctcc tgttattttt cgtgccattt ccacatgata
     2761 gcgatgtcca ttgtgaaaag gattgtactc tacgataatc ccgcagcttt tcatgcgcat
     2821 cctccttcat agttatcgat aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg
     2881 aaaaaatacc cgaactatat aaactattct cgcaaacaca atacttctgc gacgaaaagc
     2941 tgcactgcaa gagcaatgcc tatatcgttc ggatatataa tttatgttga agatttattc
     3001 ttttcttgtt aactcgagca ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg
     3061 ctttgatttc tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa
     3121 gccgctgttt tccagcattc ttaaataact ttccaatgga taggtacgct cttgatgtag
     3181 ttcatcttca cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa
     3241 atgttcgatg ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag caaaattatc
     3301 cccttgataa tgatagctat attctggaaa aacctcatct acttgatagg tggagtgtac
     3361 gtcaaataaa aatcggccat tttcttctaa cgcttgatag acttcatcaa acacttgctg
     3421 tacctcttgt ctatttttca tatagcacag cgaatcagaa aaacaggtaa ttgcctgata
     3481 ttgtccgatt tctgataaat cgagcatatt gccttccaca aattggatcg atgcgtcttc
     3541 ttcatatgct cgctggctgg caatcatcaa catctcttct gaaaggtcta accctgtcac
     3601 tttatatcca tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg ccagttccaa
     3661 aatatttttt gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag
     3721 tgtttcgtcc atcacttcat cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc
     3781 caaccattca ctaagatcga ctagcggagc atctgaccat agtttttcca gattatagaa
     3841 atttcgttct ggcgcatgga aaacatgtac gatcacatct ccaagatcga tcaataccca
     3901 ttttccgcct tcttttcctt caatacgttt tacttcatat ttgttttctt cttctttatc
     3961 aatgatttct tctgtaattg cgttgatttg acgttcgcta tttgctgaac agatcatgaa
     4021 ataatctgcc aacaaggaaa catttcgaac gtctaaagcg ataatgtctt ctgcccgttt
     4081 tgaatctgct gcttcaatcg caatttttaa ttgttcttta ctatctatga tgatttcctc
     4141 ctaatatgag cagcttattt ttttgctacc cagtaattat atgtttcgat tgtttttgga
     4201 taaatctgtt gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc aaatgctaca
     4261 gcttcgtcta gatcagccca tgcaatggct cgtgcatctt gtaccccagg aaaatctcga
     4321 cctggttcaa tatagtctgc tacataaatg attttgtcaa ggagactcat cttagcagcc
     4381 ccagtcgtat gcacccggat tgcatgaaga atctctgcat cggtgattcc taattcccgc
     4441 tctacaaacg aagcgccaac aagaccatgc cagattgcat tattataatt caataactcc
     4501 ggatcaaaac cgtctcttac gattaccatt ttgaactcct catctggtcg ttcttttgca
     4561 taatcatgtg tcaatgcagc aatactagct ttttcaggag aagcaccgta ttttttggca
     4621 agagccacgg ctgtttcttc tactccaagc acatgcttga agcgttgttc gctcattctc
     4681 atctgtattt tttgcatcag gttttctcta gaataagcag tatattcatt actatagtcc
     4741 atattcatac aacccttcat tttgaatata atcaattact ttatctggaa caaggtagcg
     4801 gatagaacag ccttctttga tcttttgacg tattttcgtg gaactgatgt cgatctcagg
     4861 tacatctacc caaattacag gatatggagt atccgttgtg tatcctggtc gacgtatacc
     4921 cacaaagttg accatagaag tcagctcatc gattttatac cattttggca aatattctac
     4981 catatcccca ccgataataa agtaataatc tgtatccgga ttgttttgcg tcaattcctt
     5041 catagtatca taggtgtaac ttttcccacc acgagctaat tcaatcgttt caatctgcaa
     5101 aaaaggattg tcttccaccg ctaattccaa catgttgagt cgatgttttg catcaatcgt
     5161 ctgtttctca tcaacatgag gtggctgata agaaggcatc aaaaatacgc ggtcaagacc
     5221 taaattccgg ccagcttgtt cagccatcac taaatgtgca aggtgaacgg gattgaatgt
     5281 tccgcccaag atccctactt gctttttctt ctctaagaat tgaggcattt cttcaggaaa
     5341 taattgtgaa cgaacaaatg tcttagcttg cgtattcatc atggtcactc cttatattgc
     5401 ttttacttct tttgatagcc tttgatactt ttcttttgtg gaaggtttgt ataatacaat
     5461 aacacgtcca atgatttgga cgatttcgca atctatttct ttttctaata cttgagctgc
     5521 ttcttctgca atctcatctg tattttgaag taacgatact ttgatcagtt ctcttttttc
     5581 taaagcttct tcaatttgaa cgatcattgc tgaattgatc ccaccttttc cgatttgaaa
     5641 aatcggctga agatgatgcg ctttgctacg taaaaaacgc ttttgttttc ctcttaaact
     5701 cataattttc cttcttcctt aaatcaatgc tttgcgtttc aatacatcta caccctttgg
     5761 cgcccagccc gcaattacta ctggcgctgt tacagtgatc cagcctagac cggcaaaaac
     5821 gatgtctgtt ttttctttca cagaaaactc aaaacgaaca agctccggaa atgcctcgac
     5881 ttcatcagga cgaggaggct gtaataatcc acctacatgt ttttgataaa attcgtctgc
     5941 tttttccagt ttcgttcgat gaatatttac atcatttgac acataagcaa taaatgaagc
     6001 acgattgccg ctgataaaat caaatcttgc aagtccgccc aagaaaagtg tttgcccttc
     6061 attcagctga taaactttag gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt
     6121 tttacctaag tagtgcgcca tttgatggcg atggataatt cccggtgtat caatcaaaaa
     6181 atgcccgtca tctaaaggaa tctcgatttt atccaaggtc gttcctggaa actgcgaagt
     6241 tgtgattaca tcttgtactc ccgctgtatt tttgatgatt tgattgataa gcgtcgattt
     6301 tccgacattt gttactccga cgatatagac atcccgtcca tcgcggtatt cttcgatttt
     6361 ttctaatagt tccgtcatct catgagattt cttagcacta gttaataata catctactgg
     6421 acgaattcct gcttcgtaag cacgttcctt catccattgt gttaatttag tttttttcaa
     6481 tgatttaggt aatatatcca ctttgttccc aactaaaagt actggattgt caccgacaaa
     6541 acggtgtagt cctgggatta gtgaaccatt gaagtcaaaa atatccacca cattgacgat
     6601 caatgcatct ttttgcccta attcattcag taagcgcaaa aagtcgtcat ccgtcaatga
     6661 gacatcttgg gtctcattat aatggcgtaa tctgaaacaa cgctgacaat agacttcccc
     6721 ggaagctaaa cctttttcca aagccgtttg cggcgtgtat cctaattcac cgggtttgtt
     6781 tgtttgtata acggcaccac agccgataca ttgtaattct tctgtcattc gattccacct
     6841 cgccagccca tatcagggtg ttttgctaat agataggcca taattctttt ttcccaaaaa
     6901 cgattaaatt gtgtcttcca actatctgtt gcgataatgg gttttacaag gatgctacgt
     6961 atccctgcac gattggctcc acgtatatca gtcataatct gatcgcctac cattactatc
     7021 tcatcatctg ctaaatccaa ttgtttcttt gctatgttga tccctcttgc taaaggtttc
     7081 attgctcgtg caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa
     7141 ttattgtttg aaacaacaac aacagggatt cctgcatttt tcatttctaa aatccaagta
     7201 agcaactctt ctgttccatc tggattattc catgcaatca acgtattgtc taaatcggtc
     7261 aatactgctt tgatgcctag attctttaat tgggcaggtg taatctgata gatggcttct
     7321 accatccatg ttggtttgta atttgaaaac atgctgtctc ctcatctgca aaaaatgaag
     7381 gagcacataa agtactcctc aaccccgtat tatactgcat atcagcaaaa aaaactacct
     7441 ctatactcgt tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa aataaagatt
     7501 gatcacagaa aaatttagaa acaaaaaaca actaatattg gcggaacctc ttgtatcgtt
     7561 tttccagtaa tttatgctga tctaatcgaa caagttccgt caatctttca atcaacgctt
     7621 ttttcataat gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa
     7681 taactcgatc aacgattcct aactcgcgta gttctggcgc agtaattttc atcaactcag
     7741 ctgcttctgc ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa
     7801 gcacagcata aatcgtttct tccagcatcc agacttcatc cgctaaacct agagcaaggg
     7861 caccgccact tccaccttca ccaatgatga tggcgatgat cggtaccttt aaatcagaca
     7921 tttcgagcag atttctggca atagcctctc cttcgccgcg ctcttctgct cctacaccgc
     7981 aatatgcacc tgctgtattg atcaaggtaa tcaccggtct attgaacttt tccgcttgtt
     8041 tcatcagtcg taaagctttt cgatagcctt caggatgggg agaaccaaaa tttctgcgga
     8101 tattttcctg cagatcccgt cctttttgaa taccgatcac tgttaccggt ttgccatcta
     8161 atcgagcgat tccaccaaca atcgcttcgt catctgcaaa taaacgatca ccatggcatt
     8221 cctcgaaaga atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt tctttagctc
     8281 ttgctaactg aacgacatca tgtgctgtct tatccacgat ctatccatcc tttctgtgta
     8341 tgcaattcaa gaagcgtatg gattttctgt ttcaattcca tgcggggaac aatctgatcg
     8401 acaaaaccat gagataataa gaattcagcc ttttggaaat cttctggaag ttcctgcttg
     8461 atcgtctgtt cgatgacgcg tcttccagca aaaccgatca agctttgggg ttctgctaga
     8521 ataatatcgc cttccattgc aaaactggca gttactcctc cggtcgtcgg gtccgttaaa
     8581 acagttagat aaaaaagacc ttcattgcta tgccgtttga cagcagcaga gatttttgcc
     8641 atctgcatta gagaaaagat tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag
     8701 acgactggaa gactttcctt tgttgctcgt tcgaataatc tggtgatctt ttctccgaca
     8761 atcgttccca tacttcccat gataaaatta ggatccatca ctccaataat gaagggaatc
     8821 ccctgaatgg ttgctttacc agtgaggact gcttcatgaa gtcctgtttt atcttgcatt
     8881 tttttaattt tttccgtata ttcgggaaat gctagcggat ctttggttac aagatccgta
     8941 tcccattctt caaaactttt ttcatcaata atcaaagcta gacgctgcca tgcaccgatt
     9001 cgaaaagcgt atccgcaatg agggcatact ttttcttcac cgatgtcttt cgtataaagg
     9061 atatgtttgc aatttggaca ctttgcccac atgttatcag gaacagatgg cgagttagca
     9121 ggtttgctct ctctattagg attgatccga atatatttct ttttcttaaa cagagccatc
     9181 tatgcgcctc ctatctccgt ttctggtgtc caattcggta aaaattcttc ttgtaaaaat
     9241 gctgtgctgt aatcacctgc tatgactgca ggatgactaa tcaaatccat ctgaaattct
     9301 gcgttcgtga tgattccttc tgtgataaat tcacttaagg ctcgctgcat cttcattaat
     9361 gcttcaaaac gcgtatttcc atggacgatt atttttgcaa tcattgaatc ataatatggc
     9421 gggatcgtat atcccgggta aactgcactg tctactcgca gtcccatccc acctgctgga
     9481 agcagtaagt tttggatctt ccctggagaa ggagcaaaat ggaaagcagg attttctgca
     9541 ttaatccggc attcgattgc gtgtccttgg aatactactt cttcttgaca cacattcaaa
     9601 ggctcaccgg ctgctatctc cacctgtttt tttactaaat cgattcctgt gaccatttct
     9661 gttactggat gttccacttg gattcgagta ttcatttcca tgaaataaaa atcgccttct
     9721 tcatccatca gaaactcgat cgttccggca ttttcataat tgactgcttt agctgctcga
     9781 acagcagcgt ctcctagtgc ttctcttttt gtttgtgaga tcactacaga aggagattct
     9841 tccagtactt tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca
     9901 tgaccaaatc gatcacctaa gatctgaact tctatatggc gagcaggata aatgattttt
     9961 tccaaataca tatcatcatt tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa
    10021 tgctgtggga gctcttcttt tgattgaact ttccgaatgc ctttcccgcc accacctgca
    10081 gctgctttca gcatcactgg ataaccaatt ctgtctgcta tttccaatgc ttcttctact
    10141 gtagataata caccagtact tcctggaatg actgggacat ttgcttcttg catcaattga
    10201 cgcgcattga tcttatttcc cattgcatcg atcgttgcac ttttaggacc aataaatgtg
    10261 atattgcact cttcacacat ggaggcaaat cggctatttt cagataaaaa accaaatcct
    10321 ggatgaattg cttcggcttt tgtcacgatt gcagcgctca gtacttcctg aacattcaga
    10381 tatgaatcaa cggcttttgc cgggccaatg cagattgctt catctgctaa ctgtgtatga
    10441 agtgcttcgg catctgcttc tgaatatatc gctacggttt ggatccctag ttcgcggcat
    10501 gcacgaatga tacgtacagc gatttcaccg cggttagcta tcaatacttt agaaaacata
    10561 tttcacctat ccaatcataa aggttaattc cgcttcggct acttttttac cgtctacttt
    10621 agcgattcct ttgcctattc cggctgaagc tttcactttc aatatctcaa cttcaaggta
    10681 taaggtatct cccggtgtaa ctttcttacg gaattttgct ttgtccaatc cgccgaaata
    10741 agccgtcttt cccttgaatt gttcaagaga aagtaatgca actgctcctg cttgagccat
    10801 tgcttcaaca atcaataccc ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa
    10861 gggctcattg atcgttacat ttttcttagc aacgatccgt tctccttcaa ccatttcttc
    10921 cactcgatca atcaaaagca ttggataacg atgtggaatg atttctttta tttcttgtat
    10981 gttcattgtt ttccctcttt cctgctttgc ttatttttgc tgaatcctaa agagtggctg
    11041 tccatattcg acaactgact cgttttctac aaggatttct gtgatcactc cgtcggcctc
    11101 actagttatc tcattcatca gtttcatcgc ttcaataatg cagataactt ctcctttggc
    11161 aacttgatca cctacttttt tgaaagctgg tttgtcgggc gctggttgca gataaacgat
    11221 tcccacgatt ggcgaaacaa cctcgattcc atcaaatact gccgattctt tttctttcat
    11281 tatttctgga gcagttgttg cttgttcagg tatgacattg actggctccg aaactggcaa
    11341 cgtttcgttt gtttgaacca tattcatgcc tcgacttgtt ttatttttat tcatatacaa
    11401 ttcaaattga ccttcacgca aatcgaattc cgttagcgag gattgatcaa attgactgac
    11461 taattctctg atttcattga tatccaaaat tattcctccc agcgtttcat gcacagaacc
    11521 gcattatgtc cgccaaaccc taatgaatta ctcattgcat aagtaaaact tgcaggtttg
    11581 ctttcgtttt tgacaactgt caagtcaatc tcaggatctt gtttttctac attgatcgta
    11641 ggtgggataa attgatgttc taatgccagc aaagttgcca aggcttcgat cccgccggct
    11701 gctcccaata aatgtccagt catcgatttc gtgctgctga cgtaagtatc tctgaaattt
    11761 tcacctaatg cgatttggat tgcttttgct tctgcctggt cattggctgg tgtacttgtt
    11821 ccgtgagcat tgatatatcc gacttgttcc ggctgtacac cagcttcttc catcgcaagc
    11881 ttcattgctt tagatgcacc agaaccatct ggagtaggag ctgtcatatg gtaagcgtca
    11941 cagtttgcac cgtatccaac gatttctcca tagattttgg caccgcgagc ttgtgcatgt
    12001 tctaaagatt caagaactag aacgcctgct ccttcaccca tcacaaaacc attccgattt
    12061 tcgtcaaacg gaatcgaacc acgattagga tcagttgatt ctgagagtgc tgttaaagct
    12121 gcaaatccag caatccccat ctcacaaatc gttgcttctg ttcctccagc aagaatgaca
    12181 tcagaatagc catgtttgat gttacggaat gcttccccaa ttgaattcgt tccgctagca
    12241 caagctgtta cgatagctgt acaaatacct tttgcaccta cacgcaaagc aatatttcca
    12301 gctgccatgt tggcaatcgc cattggtacg aacattggag aaactcgttt tgcacctttt
    12361 tcatgtagac gaataacttg gttttcgatt gttggaagac caccgatccc agatccgatg
    12421 atgacgccaa accggtcttg attcattttt tctttatcta aaccgctcat ctccattgct
    12481 tctaaagcag cgtagattcc atagatcgaa aacatatcca ttcgtttgct atctttttta
    12541 ataaaatatt tatcgtaagg gaaatctttg acttcacccg ctaaggtaat ccctgtttct
    12601 gaagcatcaa atttagtgat cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa
    12661 aagctgtctg cgtcatttcc aataggtgaa gtgactccat agccagtaat taccactcga
    12721 ttcatttgtt tgctctcctt ttcttatccg tgcatgacca atccgccatc gacattgatg
    12781 acttgtccgg tgatatatgg attttgtgct aagaaaactg ctgtttgtgc aatatcttct
    12841 acttgtccga aacgctgcat cggaatttgc ttttccgctt gttccttgac tttgtctgct
    12901 aaaacttccg tcatatcagt tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct
    12961 ctagttgcag cttctcgtgc cacagatttt gttaatccta ctacgccagc tttactagct
    13021 gcataatttg cttgaccaat atttccaatc aacccagata cactagagag attaatgatg
    13081 gccccttctc gctgcttcat catttttttc aatacgtgct gcgtcatatt gaaggttcca
    13141 atcaaattga tatccagaca ttttttgaaa tcttctgcat ccattcgcat cacaagtttg
    13201 tcatttgtga ttccggcatt gtttactaac acatggatcg atccaagctt ttcttctgct
    13261 tctttgatca tttgtcccgc tttctcataa tcagagatgt caccggaaac tcctacgcat
    13321 tttacaccga atgcttcgat ttcttctatc ttttcttttg gaatctctcc gcgaccattc
    13381 aaaatgatat ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt
    13441 gtactacctg tgataaatac attttttcct tttacatcca taacgttcct ccttatagta
    13501 ttgctattgt ttcatcaaat gtctgcttat tctcaacacg cagaactggt attgttttat
    13561 cgattttttt cataaatccg cttaatactt ttccggggcc gacttcgatg acttgttcga
    13621 tgccgattgt ttttaactta tggatactct catagaaacg aacaggttgc atgacttgct
    13681 gttcaagtag tcccgcgatt gtttctttct tcattatttc cgttgttgtg ttgcttatga
    13741 taggaaaaga aggttcagag aattgaatct gacttaggtc cttcgccagt ttttttgctg
    13801 ccggttctaa aatagctgta tgaaaaggcc cgctgacatt tagagggatc attcttttga
    13861 accctttttc ttttaatagt aaaacagctt cgtctactgc tttttcttcg ccaccaatca
    13921 cgatctgctg aggtgtatta taatttgcag gagatacgat gccgtacttg ctagcttcat
    13981 gacagctttc ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc
    14041 cagctggggc agcttctgtc atataagcgc ctctttttgc tactaacgca actgcttcag
    14101 tgaaggatag cgcattacta gctactaatg ccgaatattc tcctaaactc agtcctaaag
    14161 ctgcatctgg gatgattcca tgctccttca ataagcgata atacgcgatg ctgactgtta
    14221 gaatagctgg ctgtgtatat tgcgtctgat ccaaacgttc attttctgta aagcaaagtt
    14281 cagccatatc gtagccgaga atctcacttg cttcatcaaa tgtctgcttt acaacggctt
    14341 cttcatataa atcttttccc ataccttgat attgtgcacc ttggccacta aataagaatg
    14401 ctgttttcat gtttttctcc aatatatcca gaatatatgt ttgattcttc ctattcaaaa
    14461 aggccgagac attcctattt caggaaatgt cccagccccc aacctaattt taatgattat
    14521 gcagtttgtt tttctacgta agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt
    14581 tcgattttca cgtcaaattc atcttcaatt tcattgatga tttggaataa atccaagctg
    14641 tcagcttcta agtcttcttg gatgtcagta gtcaatttga tttcttcagt ttcttttcct
    14701 aattcttctg cgatgatttc ttgaattttt tcgaatacca taataatata ttcctccgat
    14761 attgtttgct ttttattttt ataatttaac agtctacaac tccatgagaa tacttcccca
    14821 tgttaaacct ccgccatagc ctgtaaatat tactttctgc tgttttccta aggtgatctt
    14881 tccactagaa actgcttcat ccaataagat agggatgctt gcagcagatg tattcccata
    14941 tttatccata ttggtcagga acttttcttg cggcatcttt acttttcggg caattttctc
    15001 aataattctg aggtttgcct gatgcaaaag cagataatca actggtttgt ctttcatgac
    15061 ttcttggata ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat
    15121 ttgcaaataa tacgaacctt ctgaatcttc tgaatagaac ggatttttat tttgctggta
    15181 gccagcggtc aatgagctac ttctggtacc gtcagctgct aatttttctt tcagcaattt
    15241 tttctgtgga ttcgcttcta agagaatccc acctgctcca tcgccaaaca acacagcagt
    15301 cgtacgatca gaccaatcga ttagcttaga tagtgtttca ccaccaatca ccagtccctt
    15361 agttgagccg cagcggatca ttttttcagc catgcttaac gcataaacaa atcctgagca
    15421 cgcagcactg atatcaaaag cgatagcatg agttgctccc aatttccctt gtacttgtgc
    15481 tgctacagat ggtgtcgtgt aatcgggcga cattgttgct actaaaataa aatccaaatc
    15541 tttagggttt agatttttct gttttatcaa tttttccgcc actaaaatgc ataaatcaga
    15601 agtctcctga tttgttacac atcttcgttc acgaataccc gtccttgacg aaatccattc
    15661 atcagaggta tccatcatct tggaaagatc attattagta ataatctttt caggaaggac
    15721 gctggctgtt gcagtaatca ttccaaattg ttccattaaa tatcctcact tatagtcttg
    15781 caagaagtcg tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct ccgtagccat
    15841 tccgtctaag atgttcttga ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa
    15901 ctttcctttt ttcgttagtc ccaactttac aacgcggcgg tcatcttcac tgcggatacg
    15961 ttcgacatag ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat
    16021 tgatagttct ttggctactt ctgaagttgt ttttttcttg tacatcccga ttgcctcgat
    16081 cgtgtgcatc tctgtgattg acagatcttt gaattgagat tttttcagct cagactcttc
    16141 gattgtcaaa atatcattga agacactaac aagataatca tttactgttt ttaaatctgg
    16201 ttccatgttc caccgtccac taattagttt gattatcaaa tcttttgatg ttcaaactat
    16261 atcttaactt ttttcaaaat gcaaccttaa aagatcattt ttttactata tgagcttttt
    16321 ttaagatttt actcatttaa aacaactgat aaatcagcat ttataatttc ttttattaga
    16381 aaaatcatct atctcacata ttttgattat caaagtattt tctgtgaaca gaactataga
    16441 attccctaaa acttgtccat tatttcaaaa tttgttatag tctttttatt caaagcgtaa
    16501 aaggagatcc tatgttaaaa gaaaaaatga aaactctccc catgctgccg ggtgtctatc
    16561 ttatgaaaga ccgagatggt aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata
    16621 gagtaagcag ctattttcat cagaataaac agcattcaaa aaaagtatta cgtatgattc
    16681 atcatataac agattttgat ttcgtcgtag tagacacgga attagatgct ttattgctcg
    16741 agtgtcaatt gatccaacat taccgtcctt tttataaccg acaaatgaat tatttcagta
    16801 attataatta tgtacatatt accaataagg gatttgtatt aacagatact cctacggctc
    16861 gaacatacgg gccttttcgt ctctataaaa aaatgccgtc tattttgcgt ataatggaag
    16921 aaacttatca aatgccttgg ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc
    16981 ctgacctaca ggaaatgact tttgaacaga aaaaaaagga actccaaggg ctgtttcaag
    17041 gccgaaataa aaagctactg acttacttga aaaaaagaca acaacatttc atctatcagt
    17101 tgaatttcga aaaagccggt atgcttcaaa aagatatcga attagttact tattttatta
    17161 gacggatcca ggaacaaaag cagtttttaa gaacaccatc gctcaccttc agcatgccat
    17221 tagcagcaga tgagagtcag aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa
    17281 caatgattac ctctggtgac gtttctcctg acttctatta tgaaaagaaa gaagctcatt
    17341 tatctttgaa acgtcaattg tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt
    17401 accgcaaaaa attagaaaaa gaacagtttg aaattgaact gttaaacaaa aaagaagctg
    17461 aaaagcaact aaattagttg cttctcggct tcttttaggc atcttcaact tcaaatgtca
    17521 attatcctaa aatttattgg attaagtcat agatttccat cgctacgata tcgatattat
    17581 caaattcgta ttgctgatga gtatctgttt cagttaattc aaatgtatcg gtcgctttat
    17641 cataagttac tgtacacttc tctacgcctt ctttttcaaa acggcgaact tgcagttcat
    17701 tatctgctga ctcgaccata gcctccagtc gttttatgat tgcgactaat tgtgaatgtg
    17761 tcatcggagg tcctcctttt cttatttctg ttatattgta tcaaaagcat agcctacttg
    17821 cattgatttt ctctaaaaaa actcgttttt tatgattttt tttctggtat ttttccttca
    17881 ggccaccaaa gtttgatcaa agcttgggtt ccgtcattct caaaaccttt atctgccaac
    17941 tcttcatata gtttcaatgc ttgggtagtt gctggcaata caagatccat tttttctgct
    18001 tcttctaatg caatctttaa atctttgata aaatgtttga caaaaaaacc aggtgtgtaa
    18061 tcttctttta aaatacgagg agaataattg cttaaagacc agttagctgc acttccacct
    18121 gaaagcgtct caatcacttt ctttaaatca agtccgtttt tttgtgcata gacaagcatt
    18181 tccgtcattc ctgtcatcgt acctgcaatc atgatctgat tagccatttt tgtatgctga
    18241 cctttcccag cagaaccatg caatgtaaat gtcgtaccaa attctttgaa taaaggcagt
    18301 acttgatcat aaacggcttc ttctccaccg accatgatcg tcagtgtacc attctttgct
    18361 cctaagtcac caccggatac cggtgcatct aacgcatctg cgcctttttc ttttgctgtt
    18421 tttgcaattt tttctgccag gcttggtgta cttgtagtca aatcaactaa aatatgtcct
    18481 gagatatctg cagaaaaaat ccccgactgt ccatagtaga tttcttctac atctttgggg
    18541 taacctacca ttgtaaagag aatattactt tgttctgcta ccgcttttgg tgtatctgcc
    18601 cagatagctc cttctgttac taaaggatca gcttttgctt ttgtccgatt atagacagct
    18661 acttcgtgtc ctgcttctag tagatggcgt gcgacagcac ttcccatgac gcctgttccg
    18721 ataaaaccaa gtttcatttt ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta
    18781 gtatacgcta gaaacaagga ttttttcata aaaaatgttt gaattttatc ttttaaatga
    18841 atttaatttt attttgattc gttcaatcga gtcttcttct tcttttcttc gcatctcttc
    18901 ttcatgataa acaaccatgt cttgataaaa ttcgatcata gcttcgccga aatgtgtaga
    18961 tgaaatatca taaagtttag cttctaaaac ttttggatct ttaggaactt cactggaaat
    19021 ataatcaata agtgctggtg caaagtcttc gtcggtttca aaagttttcc ctaatgaagg
    19081 atgatcgaac aatcggttca aataagcatt tccttccacg acacaaggtg ttccggctgc
    19141 catcgcttct gtataagtaa gcccttgtgt ttctgaagta gaagcactga caaaataatc
    19201 tgccgcttta taataaagtg ctacatcatt gttgtccact tctcctgtga actgcacaaa
    19261 ttcttctaac tgcagttctg agatcaattc ctctaactct tctacataag gtccttttcc
    19321 tacgataact aatctggcgt taggataagc ggagatgacc tgaggaaacc cattaatcaa
    19381 ggcttggata tttttctcat aagaaatcct gcttaaagaa agaatcatca aatggttttc
    19441 ctgaagacct agtgactccc ttaatttgct gatatcctgc tgagtgatat cagaacgttt
    19501 gaatttttca atgtcgatac cagtaggaat cacgcgaagc ggtgctatta caccatagct
    19561 gcgtaatgta tcaataacac gttcacttgg acaaactaca cctgtcgtat gatttgcaaa
    19621 taatctagac aagtatttta catgggattg cctgatcact tttcctttac cgatataatg
    19681 aaggtagtct tcatacatcg tatggtaagt atggatcaga ggtattttca acttcttagc
    19741 taccatcttc ccaagtatcc ctgctccgaa ttctgtatgg gtatgaataa gatctagttc
    19801 tagttctttg gcaaccaaat aagcatacca catgcctcta acaacaactc ttcgatcctt
    19861 gaaagaaata aatggtacac ttggcattcg aatcacatct ttttccaact tatccgcatt
    19921 aggatcggtc gttgtaaaaa tatagacttc atgcccatgt ctttctaatt cttgttttaa
    19981 tgttttgatc gatgtagcaa ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc
    20041 gattttcatg tttttcgcct ccaaactagt ccttgacctg atttactttt tttaatttgc
    20101 tagagacttt ttcattcagt acactttcgt atacattttt aagttcttca ccgattttat
    20161 tgatacttcg ctcttttgct gtttgataac cggcttgtgt cgtactcggt agttcttgat
    20221 tggtaatctg ttggatcagt cggataaatt cttcgtttgt cttccccata tagcagttct
    20281 tgctatcttc taaccagcca ttatacactg gaatatctcg aacaagtacg ttttgtttgc
    20341 ttgctaaagc ttctaaaaca acgatccctt ctgtctcttc ataagaaggg aaaaagaata
    20401 gatctgcacc tgaatacgca ccttctatga tctctccacg aatgtaacca ggaaacagga
    20461 cattttcagg atgatcttct tttacaattt tccggatgtt ccgaggaatc gagtacatgg
    20521 gaacatgacc aaaccaaata aatgtgtatt cgggcatctt cttagcaatt tctacgaaat
    20581 caatgattcc tttacgttcg aaaaacaaac cgacacaaat gattactttt tgatccggag
    20641 acagtttgaa atattctctg aacttttgtt ctttcattgg atcagggtga tatttctcta
    20701 agtcgatccc attggaaata gcttgaatcg gcacacggat accatagctt tccaacaact
    20761 gttttgaata aggtgttggc gtgatcagat agtccgcttt ttggtacaat cctaccagat
    20821 atttttttac gataggagaa agctggttcg atctgataaa ggaatttcgg aaatcttcct
    20881 ctgtagaatg cgcatgatag acaacttttt ttccttctct tctggcttta ttgaccatat
    20941 tatgactatt gattccgtat gtattgatgt gcaggatgtc ataatcttct ttttcatcta
    21001 gtgtataaga gataccgact tccgtcagag cacgtttttg atgatcaagc gcacgcccga
    21061 ttccagattt tgcaagcatc ttttcacttt caaaatataa taataccttc accttacgtc
    21121 ccccttcact tatatagtat aacatagcct acaaactgtc actcaaactt aagccctatt
    21181 tttgtctatt cttactgaca aaaacgtttt cttaaaaaag aacaaaaaaa ctggcgtagc
    21241 tcttttacaa gccacaccag cttttgatac gttgtttatt ttacatattc atgaacaagt
    21301 tcaacgactt cttccatcat atcgcattct ttcaatgcac gatcagcaag ttcagccatt
    21361 ttcgctgtgt ccaagcgttt cattaaacta cgcgttttaa gaatagatgt cgcactcatt
    21421 gagaactcat ctagacccat acctactagc aatggaacag ctgtttgatc tccagccatt
    21481 tctccacaca tacctgccca ttttccttca gcgtgtgctg catcaattac atttttgatc
    21541 aaacgtaaaa ttgatgggtt atatggttgg tacaagtaag aaacgcgttc gttcatacgg
    21601 tctgctgcca ttgtgtattg aatcaaatcg ttcgtaccta cagagaagaa atctacttct
    21661 tttgcaaact tgtctgctaa gactgctgct gctggaatct cgatcatgat gccgacttgg
    21721 atcgtatcag atacttcctt gccttcactt atcaattttt gtttttcttc ttcaaagatc
    21781 gcttttgctg cgcggaattc tttgagtgta gctaccatag ggaacatgat acgtaaatta
    21841 ccatgaacag atgcacgaag caatgcacgc atttgtgtac ggaacatttc gtcgccttgt
    21901 tcagataaac tgatacgcaa tgcacgatat cctaagaatg gattcatttc atgaggtaat
    21961 tgcagatatg gtagttcttt atctccaccg atgtccattg tacgaacaac tacaggtttt
    22021 ccttccatac cttctaatac agctttgtaa gcttcatatt ggtcatcttc tgttgggaag
    22081 tctggagaat ccatatacag gaattcagta cggtacagac caactgcttc tccaccatta
    22141 ttatggacac caactagatc ttttggtgtt ccgatgtttg cagctaattc aaaatgcttg
    22201 ccgtcagctg tgacagtttc tgcatttttt aatttttccc attctgcttt ttgtgctgcg
    22261 taatctgcac ctgctttttc aaattcagct ttttgttcat ctgtaggatc gatgattaca
    22321 tcgccttcga tcccgttgac agccaaaata tcgccttctt tgactttagc agtaatttct
    22381 tttgtcccaa cgattgctgg aatttctaaa gaacgtgcca tgatagcaga atgtgaagta
    22441 cgtccaccaa tgtctgttac aaacgctttt acaaaattgc gatctaattg tgctgtatca
    22501 cttggagtca agtcatgtgc aacaacgacc acttcttcgt tgatcattga tggatttggt
    22561 aaagtaacgc ctaataagtg cgctaaaata cgtttagcaa cgtcacgaat atctgcagca
    22621 cgctcttgca tataagcatt atcttccatt gcttcgaaca taccgatata catatccgtt
    22681 acttctttta gcgctgattc agcatttacg ctgttgtctt taatattttg cttgatttga
    22741 cctaccattt ctggatctga aagaaccatc aaatgggcat caaatacttg cgcttctgct
    22801 tcacctaagc tttgcgctgc tttttcacga atttgctgca attcttcagt agatttagct
    22861 aatgcagcat ccaaacgagc ttcttcagcc gcagtatctt ctactgttgc cttactgaat
    22921 gttaaatccg gttgaactag caggtaagct tttgcaacgg ctacaccgtc actagcggca
    22981 atccctttta gcatttcaac cattattcag ataagccttc tttcttcatt gtttcaacga
    23041 ttgctgccat tgcatcagct tcgtcagcac cttctgcagt aatagtaaca tcagaacctt
    23101 gacctacacc taaagacata acgcccatga ttgatttcaa gtttactgat ttacctttat
    23161 actctaagtt tacatcagag ttaaatttgc ttgctgtttg tactaatagt gtagctggac
    23221 gagcgtggat cccagtttct gctactacgt gaaattcttt cttttccata ttaatcggtc
    23281 tcctttaagt gagtgttgtt tttatgtgag ggttttcttt tttagtaatt cagagaatcc
    23341 taacaaatgc caccctttca tctgtaaaga ttaacatttt ttttcgttag atacaacagt
    23401 tttcattcaa tttcagataa aagtattcaa attattccgg attctttttc atccttttct
    23461 tcacatttta taacaaacgt gtttatttcc tttttcttgt cgctttctct tctcctgacg
    23521 tttatcattc tttcctttga ccctctttgt tttcatatga aacgaattac ttgcttccct
    23581 ttttattttt agtataataa gagtcaaagg tcaggaatgg tcaatagaga tttcaccttt
    23641 tctacctcat aaatctctta aaaaactagg ggttattctt ttacctcatg aaaaacgaga
    23701 tgtaaaatat ggatgtaccc atttttgtga aaggacgtga atgattcatg ctttgtcaaa
    23761 actgcggcaa gaatgaggca acgattcact tgtatgccac tgtcaatggt cagcggacac
    23821 aactcgatta ctgtcagagc tgttatcaaa aaatcaaaaa tcaacaaaat ggaggtctga
    23881 ctaatatggc ccaaaatgat ccatttggat ttggcagttt ggacgatctt ttccgttcca
    23941 tgtctcgcca aatgcaacag caaggaaatt atgagcaaac accaccaaca caatttggcg
    24001 gaaacaacaa cttcaatggt ggtcagccac ctcaaggcaa tacagaaggt ttattgggag
    24061 aatacggaat caatatcaca gaacaagcac gacaaggtga tatcgatcct gtgatcggac
    24121 gcgatgacga aatcaaacga gtcattgaaa ttttaaatcg ccgaaccaag aacaatcctg
    24181 tcttgattgg agaaccaggt gttggtaaaa cagcagttgt tgaaggttta gcacaaaaaa
    24241 tcgtagatgg tgatgtaccg caaaaattaa tggacaaaga agtcattcgt ttagatgtcg
    24301 tatctttagt ccaaggtact ggtatacgag gacaatttga agaacgtatg caaaaattga
    24361 ttgaagagat ccgtcaagca gaaaatgtga tcctattcat agatgaagtt cacgaaatcg
    24421 tcggtgctgg ttctgctggt gatggaaata tggatgctgg taatatttta aaaccagctt
    24481 tagctcgagg agaattgcaa atggttggtg ctactacctt aaatgagtac cgcatcatcg
    24541 aaaaagacgc tgctttagaa cgtcgtatgc aacctgtacg ggtagatgaa ccaactgtcg
    24601 aagaaacgat cagtattttg aaaggtttgc aaaaacgtta tgaagactat catcatgtaa
    24661 aatatactga tgaggccatc gaagcagccg caactttatc caatcgttac gtccaagatc
    24721 gcttcttgcc ggataaagcc atcgatttgc tagatgaaac aggttctaag aaaaacttga
    24781 cgatccaaat cgttgatcct aaaacaatcg agaaaaaact gcaagaagca gaagaacaaa
    24841 aagttttagc ttctcgagaa gaagatttcg aaaaagcagc ttattatcgc gatcagatca
    24901 ataaacttca aaaaatgaaa gaacgtcagc tcactgaaga ggaaacacct gtgattaccg
    24961 aaaaagatat ggaaaaaatc gttgaacaac ggacaggtat tccagtgggt gaattaaaag
    25021 aaaaagaaca gacacaactg aaaaacttag cagatgactt gaaagctcat gtcatcggac
    25081 aagataacgc tgtagatcga gtagcgaaag cgattcgccg aaaccgtgtt ggattaaaca
    25141 aacagaatcg tccaatcggc tccttcttat ttgttggacc aactggtgta ggtaaaacag
    25201 agcttgcgaa acaacttgcc tacgaactat tcggttcaca agattcaatg attcgtttcg
    25261 atatgtctga atatatggaa aaacatagtg tctctaaatt gattggttct cctccaggtt
    25321 atgtcggata cgaagaagct ggtcaattaa cagaaaaagt acggagaaat ccttacagtc
    25381 ttgtcttgtt agatgaagta gaaaaagctc atccagatgt tttgcatatg ttcttgcaaa
    25441 tcttagatga tggtcgattg acagatgcac aaggaagaac ggtcagcttt aaagatacga
    25501 tcatcattat gacaagtaat gccggaactg gtaaagtaga agcaaacgtt ggttttggtg
    25561 cggcacgcga aggtgttact cgttctgtat tgaaccaatt aaacaactac tttacaccag
    25621 aatttctgaa ccgttttgac gggatcatcg aattcagtgc attatcaaaa gaaaacttga
    25681 tgacaatcgt cacactaatg ctagacgatg tcaaccaaat gctggcagca cagcaacttc
    25741 acatcgaagt tcctacgaat gtcaaagaaa aattagttga cttaggctat gatccatcta
    25801 tgggtgctcg tcctcttcga cgcacgatcc aagaacaaat cgaagatggt atcgctgaat
    25861 tttatcttga tcacccttct atccatgagt tgaaagcaaa actggataaa gatggaaaaa
    25921 tcatcgttac ttctaaacca gaacgtttgg ctaaagaatc agccgaagaa acggctgaat
    25981 aaatccagtt ttatagcgtg agacggtatt tctttgagaa atgccgtctc attctttttt
    26041 cagaaaagtc aaaatactgt tcattttata aaaaagaaat tataataaga gaaacaagca
    26101 attaaaaata cataaaagag gtgagcgcca tgaaattggt aaatgtaaca aacagccatt
    26161 cccgtcttgt attaaaccag cttgaaaaca ctgatgccca tctagtcaag gtctacacag
    26221 ccggaaatac cactattgtc tacactgaag cccctgaaca taatgaaatt ctgctgatca
    26281 atgacaaacg taaaatccag ccgaaagaaa tcgaagatgc taaaaattat ttcttagata
    26341 aaatcaaaga cgccgtttac cacgaggatg aaatcaaagt aatcgaattg gcaggccttg
    26401 ttgaaatctc gattccaaaa catgaattag gcgctacatt agccgtgtaa acaaattttg
    26461 aacataataa ttaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    26521 cacgtataaa cggcgaaagc agaagcaact ccttcggaaa taagctgaaa ttcacgaaaa
    26581 ttgcttttca aatttttgtg aattccagct tatttcttgg agttaaacgc ttctgtctca
    26641 gcctcttttt ctttctagct ttttgatttt tctcttttct tttgtacttt tctcttttca
    26701 ttttcagttc tacgcgtgtc tttattttta actttttcct ctttatggat gattgatcgt
    26761 actttttgtg caatttgctc gtttttgtcc cagtctttta catctaataa tcgacataat
    26821 cgtaacatct cttctttttt ctgttttttt ctttcacgtc tttgccattc tgtttccatg
    26881 tgtcacactc tcctttcttt aatcatatgt acgcaaaata atttttatat ttttttgatt
    26941 agacatttct atgattttaa tatagtaaaa agagccgtca cggaaaatcc gtcacggctc
    27001 taaacaaaat atgagaggct gtttttgttt ggctattctt attacttgat gcagggcagc
    27061 ttgtgtcaag acttctttac atcaaacttt ttaattttac attgggatat ttatccgcaa
    27121 accagcgttc tgcaaattgg ttctcgaata agaacaacgg ttggtcgaaa cggtcacgag
    27181 ccaagatatt gcgacttgaa ctcatcttct catccaaatc ttctggatcg atccagcgag
    27241 cgattttgtg tcccattgga gacatgatta cttcggcgtt atactcattt aacatacgat
    27301 attggaatac ttcgaattgt agctgaccaa cagctccaat gatatattct tccgttagat
    27361 atgttttata aagctgaatc gctccttctt gaactaattg atagattcct ttgtggaagg
    27421 atttttgttt catgacattt tttgcagtca ctttcataaa cagctctgga gtaaatgaag
    27481 ggagttcttc ataagcaact tttagttttc cttcgaacaa tgtatcccca atctgataat
    27541 tgcctgtatc atatacaccg atgatatcgc ctgctactgc tttttcgacg ttttctctcg
    27601 aatctgccat aaattgtgtg acattgctta atttcagttt tttatttgta cgttccaacc
    27661 agacatccat tcctcgttcg aatgttccag aacagattcg gacaaaagcg attcggtcac
    27721 gatgggctgg attcatattt gcttggatct tgaatacgaa acctgaaaat tcttcctcat
    27781 aaggacttac catctgatca tcttgtgttt tgtgtccata aggagctggt gccaattcaa
    27841 cgaaagtctc taaaaaagtc tgtacaccga agttagtcaa tgcagaacca aaaaatactg
    27901 gtgtctgatc gccacgagca attttttctt gattaaatgc atctccagcc tctttgatta
    27961 gttctacttc ttctaagact tgttcatata aggaattttc agttagagga tgattttcgg
    28021 gaagcagtcc atcttcatcc aattctacta atcgttcatc ctgataattt tccggacgat
    28081 aaaactctac acgattatgg tagatatcat aaagaccttc tagcccttta cccataccga
    28141 ttggccaatt catcggataa gattcgatat ctaatagctc ttctaattct tctaacaatt
    28201 ccaatggttc acgaccatca cgatctagtt tgttgataaa agtaaatatc gggatgccac
    28261 gtttttttac aacttggaat aatttctttg tctgtgcttc gatccctttt gcactatcga
    28321 ttaccatgac cgcactatcc acggccatca aagtacgata agtatcttca gaaaaatctt
    28381 catgtcctgg tgtatccaag atatttactc gtttcccttg ataatcaaac tgcataacag
    28441 aactagtaac agaaatccca cgttgtttct cgatttccat ccagtctgat ttggcaaaat
    28501 tacctgtttt tttgcctttt acggtcccgg cttgacggat agccccacca aacaatagta
    28561 attgttctgt aatcgttgtt ttcccagcat ccggatggga aatgatcgca aaagttctgc
    28621 gattgtccac ttgttgcttt aattttggat tattcataac ctaactccat ttcttccatt
    28681 caattattta tattttctct aggcacagat tgtccgatct cttaaagaaa gacaatttca
    28741 tatagggctg tgacaaaagc cgtgtagcag tctctttttc cgagtaaacg gcgttctaga
    28801 gacagcctat cagcaataag cccacccaag ccaaaaatac gaaaagctgt ttttgcttgg
    28861 ctgctcttat tacttaatgc aggatagctt tttcacaacc tttgtcaact actacaactg
    28921 aatcagtata gcatggattc agcgaatgaa aaagccacca aaccataaga gtcaaatatt
    28981 tttttgtcag ctgactaaaa gaaaagacgt gttgcttgtt tattcaagca acacgtcttt
    29041 cataaccaag tttccaatta tcttcttggc tcgtcatctt cgaattcttt tctgaagtat
    29101 cttcgttctt cttcaggttc cgcttctgtt tcttccggat catgaaaaat gacacggatt
    29161 ttaaggattc gagaaccttc catttcttca gaaaccagcg taatattatc gacatcaaac
    29221 gataactttt cgccttcatc cggaatcatg cctaatgctg taatcaaata accagccatc
    29281 gtatctacat cactcatatg cagatcactg tcaaaagctt cattgaactc atcaatcagc
    29341 atacgtcctt ggatgatata ttcgtgttca tcgatttttt cataaagatt ctcaacttca
    29401 tctgattcat catcgatttc tccgacgatt tcttctaaaa ggtcttctag tgttgctaac
    29461 ccaacgacgc cgccatattc atctaataaa atagccatct gattctgcgt ctttttcatt
    29521 tcatataaca gatcatcaat aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt
    29581 ttcttgatat ctaaattatc aaagccgaat ttatgggcag cttttagtaa attctttgta
    29641 tgaaggattc cgactacttt atctttgtct tcgttataaa cagggattct tgaatagttt
    29701 tcggataaaa cttcattcac attttcttcc acagtgtcgt tgatatcaat cataaatgca
    29761 tccgttcgtg ggaccatgac ttctcgggca actttcgtat caagagaaaa aaccccttgg
    29821 agcatttcta attcttcatt attcaatacg ccttcgtttt caagcatata acgcatttca
    29881 tctctggtca tttttgaatc ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa
    29941 cttgtggaag cagaaagcag ccagacgaag ggcttagcta ctacaccaat caagcgtatc
    30001 agccctgaag tgaactttgc gacttcttct gatttattta aagcaatacg tttagggtac
    30061 aattctccaa aaacaatcga tacataggtc aaaatagcca gcacaatgat atttgcaata
    30121 ttttttgccg ctgtgccgcc acctaatacc ggagctaacc gttccgataa agtattagct
    30181 agtgaagcac cagacaaaat gttgaccaaa gtgattccta cttggattgt cgataggaaa
    30241 ttatttggat tctgcaatat ttttaaaagc ttttgagatt tcacatctcc ttcatcagct
    30301 ttttgctcga ttcgattctt attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa
    30361 gcattgatca atgtcaaaac gatcaataat aaaatctgcg ctaacagcga ctgactctca
    30421 gggtcagcat tcataatcgc attctctcct tataaaatta atttttagtt tacgatagaa
    30481 aaaaacgtgg cactatagcc acgttaaaat ggtatcacaa agcaattttc tttgcaataa
    30541 aaacaagtgt actaaaacta cctacgaggt cgtagcaatt ttcttcttgt tttcattttg
    30601 cttgtcttct ttgacgattt tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa
    30661 aggaacacct aagaagatac caagcaatcc ggcaatattt ccagctacta gaagaacgat
    30721 aatgatcgtt aatggatgga ttttcagtga tttcccaatt acattaggat aaatgatatt
    30781 cccatccaac tgttggacga ttagcacaac cacacaacaa agtaatgctt ttaccggatc
    30841 attgaagact gttactagaa atgcaggtgc tagtcctaga taaggtccta aatatggaat
    30901 taggtttgtc aatcccgcaa tgaccccaaa taagaaagca taatccacac caattaccat
    30961 atatcctaaa aatgtgaagg taccaacgaa taaacattcg attgcttgtc cgctaatgta
    31021 attagccaat gttttattca gttgccccaa taactccaca atttggtttc ttcttttttc
    31081 tgggaaaaac tgttggatgt ttggcactag tttttctccg tctttcaaca tatagaacaa
    31141 gatgaatggt gctgtaatga ttactacggt tgccgaagcg atcgttccaa cgatcgaacc
    31201 taaactattg gatactccac taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa
    31261 ttgctcaaaa tatttattga tatctacttc cttgaacaaa ggcaattcgg ctacctcttt
    31321 taaccagctt tctacctgtt tgatgaaatc aggcatatta gatgccaatg aggaaatttg
    31381 ctgtactaga cttgggatga cactaagtaa aatccaaata attgctgcaa ctaaaaggag
    31441 cagcacaatt gcgacagccc atattctttt gatttttgct ttcatcaaca gcttgacaat
    31501 aggattcaat aaatagtaaa gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa
    31561 aaatgtcccg atcggttcga aaataaagtt tatttctgat gaaatcatga tcaatgcagc
    31621 tatgatcaat aattcaagcg accaaaacat tagtttagac tgtctaagtt tttcaaacaa
    31681 tagatttcct cctttctttc ttactagtat atattatatt cgttaaaaat tcttttattc
    31741 tttttttatt tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata
    31801 cgaaaggaag gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt
    31861 tacttagatg ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg
    31921 tctcgagaaa tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa
    31981 aaagaaccgc aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag
    32041 cggatggcga ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa
    32101 gcagaaaact ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca
    32161 gctgaaactt tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct
    32221 ggcatgcctc atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa
    32281 aacagcaagc ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta
    32341 cgcgacaatt gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca
    32401 tctccattat tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata
    32461 tcgttcgggt aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt
    32521 ttctcgccgt caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca
    32581 tcgtccagca aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc
    32641 attgctatta cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt
    32701 acccatcggc gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag
    32761 tgttcgcttg tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc
    32821 ttgtaacttt ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct
    32881 taagcagcat tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata
    32941 tcaataaaaa tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga
    33001 tctaccagtt tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac
    33061 cacttcgaag ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa
    33121 aaaaggagaa catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt
    33181 gtctgccaaa caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact
    33241 aggtcatcaa acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac
    33301 tcctcttgtg tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta
    33361 tctctttctt caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa
    33421 acgtcttttg gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg
    33481 ttagctaaac aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag
    33541 atagctgaac ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg
    33601 attttccgca tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca
    33661 atgcaaaaca atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt
    33721 ccaaatgcga tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta
    33781 atttcttcag gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca
    33841 gcttggatgt tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt
    33901 tcttctggag catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa
    33961 taatcgatca ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga
    34021 taaggatcac gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg
    34081 aactgatctt gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact
    34141 ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata
    34201 taagctttgc tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca
    34261 tagtttatct ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg
    34321 aagatcagct atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg
    34381 ttggattttt ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc
    34441 ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc
    34501 aatgatttca ggtttcaaac ctttttgctg catctgctgc gctaatttct ttggtccttt
    34561 gtcacttaag cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag
    34621 gtccaattct tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc
    34681 ttttaagtat gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc
    34741 taggtgaaaa ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc
    34801 taattcccgt ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc
    34861 ttctccttca gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac
    34921 ggttaacata aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt
    34981 aatcaggtat tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc
    35041 agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa
    35101 tggtgaagga atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa
    35161 agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat
    35221 aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg
    35281 cggtggctgt caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct
    35341 gcttttgcaa gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc
    35401 gacgatcggg atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa
    35461 aaataagcag actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt
    35521 ccctataaca gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt
    35581 tcaactattg aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt
    35641 ccgcacaatc atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac
    35701 tcgcagtaaa aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc
    35761 cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga
    35821 tgaaacgatc cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt
    35881 cgatctttct cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg
    35941 cgaaggaatc aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg
    36001 agttgggacg atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac
    36061 cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac
    36121 tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca
    36181 gcctgatggt atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc
    36241 attattgaaa taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc
    36301 cagagatctg gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat
    36361 gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat
    36421 gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata
    36481 gctcttcatg ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt
    36541 tatttaaaaa aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat
    36601 attcacgtct atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa
    36661 aatcacatta tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg
    36721 tggaacagtt gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg
    36781 ttgattagga ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg
    36841 tgctgttaaa ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct
    36901 gttagatgtc tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc
    36961 atcaatgaat gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac
    37021 attttgattt aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt
    37081 aatataacca tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc
    37141 aataaacaat aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat
    37201 tgtaaacgct ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa
    37261 aaaaggtgaa aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag
    37321 aaaacatgag aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt
    37381 tcaacttttt aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt
    37441 cttgctgtga gccttcatgg taaactaata ttatagaaag gagttagtaa aatgaagatg
    37501 ataaaagata ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca
    37561 gggttgctta gcggaaatcc cggaaaatat tactattctt tgcaacttcc accatttgca
    37621 ccacctagct ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct
    37681 ctttatttgt tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt
    37741 gtgattcagt tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt
    37801 ttcattgcag caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag
    37861 ttgtggctgc atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta
    37921 ttcgcaactc tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc
    37981 ctgtaattta acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg
    38041 gcactcttta ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta
    38101 ttctggtcat aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta
    38161 tcttgacttg gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga
    38221 tcttggtact gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta
    38281 ttcgatccat caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa
    38341 gcattctcat ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc
    38401 agttcatttt ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg
    38461 atcatttgtc cttcgccttg caactggtct tgtttgattg tttcaaacgc cggggtgtag
    38521 ttcgtcgcta catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact
    38581 gcttttaaaa tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt
    38641 ttcgtcacta cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt
    38701 cgcattcgag aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca
    38761 tggacaccat ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg
    38821 atcaaatctt ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc
    38881 agttgctcaa ttgaatccat ggccatttcg acaccaccaa atgcgtaggc atgatttaat
    38941 ttatctaccg ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc
    39001 gttgattttt tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt
    39061 tccgttcttc ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg
    39121 tcatcaatgc ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt
    39181 ttgtttactg tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca
    39241 atcagcaaaa cgaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata
    39301 cacctctgtt tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca
    39361 ctttttaatc ataagaaaac tttaaaaaac atttttgtgt aacgattatt attttttaaa
    39421 atttctcaaa attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca
    39481 cgcagttcgc aatcgtttta tcttttttta tgtatttttt ccagaaaaag cgaacaacaa
    39541 ggtattttcc aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac
    39601 agtctttgcc taccgttcag attatcattc gatttcacga caatttcttc ctatgactgg
    39661 tccttttgtt aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa
    39721 atgactcgaa ctagtgattg cattaccatc atcttttaat ggatagatat gctcctcttc
    39781 aaagtaaaac gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta
    39841 gtggtttcac aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat
    39901 tactaacgaa gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat
    39961 gcatttactt agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct
    40021 aagaagtcgt gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt
    40081 ccgcgtgcac cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag
    40141 ccgtctcctt gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata
    40201 aacccaaatc ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatat
    40261 cctccaaaaa agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc
    40321 tagcaagctt tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa
    40381 tggttatttt gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa
    40441 tgctgcaata taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac
    40501 gtccaaacct agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga
    40561 ggtaatagct aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc
    40621 agcggctgct tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat
    40681 cgcttctttt atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa
    40741 aaatgaatgg ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg
    40801 aatagcgtcc atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt
    40861 ctcaattgct gaatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg
    40921 catcgtttct tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa
    40981 tgtgtaagtc ataaaaaaat tcctcctcaa tgttttctta ataatgtcta cattacaaag
    41041 agtaacaaag agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc
    41101 tacttcttct tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt
    41161 cgcaaggata acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac
    41221 attgatcaac aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa
    41281 gaacttaccg cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca
    41341 aagtttgttc gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa
    41401 ggccatactt aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt
    41461 agccaaacca ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata
    41521 tccttttaac tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta
    41581 tcatactcta tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc
    41641 tttgcctttg tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa
    41701 gaaattatac gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata
    41761 tgtccgtcga aaataattat cttttcgcta tactgccaaa tatggacatg atgaacatcc
    41821 tttaccccat cgattgcttc aatttcttgt tccatttttt catagtcgat agcaggtgca
    41881 ctttgtatca aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat
    41941 atcgaaatga tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa
    42001 attgctccga taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta
    42061 gattttatat tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc
    42121 gcgagcaatc caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa
    42181 gtgaaccgct tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag
    42241 ttgatgaaag cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct
    42301 tcttttcggg ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg
    42361 ttatggaaag catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct
    42421 gataaagtga tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt
    42481 ggctgtgtag gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga
    42541 ataacatttt tttgatattt caccaatcat tcagctgttt ccttaaagca cttgtatttc
    42601 acttgatact tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca
    42661 acaaaacaat taattatcag ttcctttata cgattcaaag acctaaagga ggcgagaaat
    42721 gtcccatttt ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca
    42781 atctcttttc aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct
    42841 actcgcattc ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga
    42901 ttcatttacc aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa
    42961 caggacatct ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa
    43021 cttatcatag ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta
    43081 gaacttccct acaccaatga atctttaaaa aacttaacag gtaagcagct ccgttctttt
    43141 ttaagtgaag catctatacc actttggata aaagtcctga catttatatt ttctatttat
    43201 cctagttttc tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat
    43261 gcacgctttc gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca
    43321 atttcattgc ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacact
    43381 agcgccttca ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca
    43441 aaaatatcga tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt
    43501 tagaggttgt gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc
    43561 aaaaaacaag caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa
    43621 ggcgggaaat taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa
    43681 tttatcgaag aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca
    43741 gaggatctac aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta
    43801 ttagcctctt ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc
    43861 cttgagactt tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa
    43921 tcgattacgg agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca
    43981 caaaaggcac agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa
    44041 gggaaaaaaa tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc
    44101 aaagcattag acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg
    44161 gaaaagactt tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc
    44221 aatatcttac ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac
    44281 tttgatccaa aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat
    44341 ctggtgcagg ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa
    44401 gagcctcttc cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac
    44461 attgcaggtt tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg
    44521 aaaagctact ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat
    44581 taatagctga aaacaatatt ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc
    44641 ctctcttttt ttattgtttt ttaaagtgat aggttggctc ctccaggttg ggattataat
    44701 caacaagtaa atcatatcct ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg
    44761 tcccatcaat ttccactttc tttacaggat gttctggctg ttcaactgac attccaatcg
    44821 aaaaaccatc atgaacctct gttttgccgt agacttttcc aaaaaaacgt attccgtaag
    44881 tttctggcag atcaatttct gagtcaaacc acttttgtgc ttttggcgtt accgttaatt
    44941 tcatcccaac aacctacttt ctcttttctt tctattgatt catcttatcg tctagaatat
    45001 ccacatcgcc tgtctctcca ataaccagta gattatcatt ttcacgaaca tattcatctg
    45061 ctgcaggaga agcgataacc gtttgatcta ctcttcgtat ggcgactact gttaagtcaa
    45121 aacgttgcct gaaatttaat tcagctaatg ttttgttgaa aaatttagga ttggagactc
    45181 ggatttctgc taaagaatat ttatctgaca actctaggta atctaaaata ttctttgaca
    45241 ctaaattatg cgcaatcctt attcccatgt ctcgctcggg atggacgacg cgatcagctc
    45301 cgatcttttc aagtactctg gcatgatact cattttgggc ttttgctaaa atatttggta
    45361 cgcccatttc ctttaccatc agagtaacta agatactggc ttgaatatct tctccaatag
    45421 cgacaattac atgatcgaaa tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg
    45481 catttgccac aacagcatga gtggctatat tcatatattc attgacccga tcttcattac
    45541 tatcaatagc cagtacttct tgaccagctt cgatcagtgt tcggcagata cttccgccaa
    45601 atcgtccaag acctacaatt gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc
    45661 ttttctcagt ataacaaagc ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag
    45721 ccaagaaaaa taatagttat tttctcagct tttgattatc ctctttatca tcgtttttat
    45781 ttcaccaaac catgtttgaa tgcatagata gcggcttgtg tacggtcttc cacttctagc
    45841 ttagccaata tattggaaac atgtgttttt actgttttca gtgtaataaa caattcgtcc
    45901 gctatttcct gattactttt tccttcagaa atcagcatta aaatttcatt ttcccgattc
    45961 gttagttctt catgtaatac aggctcattt cgccgactca ttttttccat catttttgtt
    46021 gtcacttctg gttctaatac tcgctcgcca cgttgcgttg cccttatagc atcagcaatt
    46081 tcatgtgcgg tagaggtctt caacaaatac cctgctgcac ctgcttcaat ggcaggataa
    46141 actttttcgt catcaataaa actggttacg ataatgattt tagcttctgg ccaatctttt
    46201 aagattgctt ttgttgattc gataccatcc atttcttcca tgactaagtc cattaaaatg
    46261 acgtccggtc gaagtgtcat cgccttctca tagccttgtc gtccattttc agcttctcca
    46321 attacttcaa tgtcttcttg aatagataaa taaaaagaga cgcctaagcg caccatttca
    46381 tggtcatcta ctaataaaac ttttatcatc cttatctatg cctccttcat caaagggacc
    46441 ttgatttcta cacttgtacc ttgaccttta aaactaatga tttttactgt tccaccaata
    46501 ccagctactc gttctttgat attgttcaat ccataacttc ccgttttcgt ttcattcata
    46561 ttaaaccctg ttccgtcgtc aataatacgt aacaaaagat tattgtctat tttgtgcaaa
    46621 tatacctcta attcattggc tttagcatgt cttaatgtat ttgataacaa ttcttgaacg
    46681 attcggaaca agtgatcctc aatggaacta gatagtttta catcttctac atcccatttc
    46741 agcgaaatct ggattttatt ttgcaattcc ttcaataact gctcgatacc ttgctttaaa
    46801 cttttctctt ctaaattgac tggacgtaga tgaagaagca gcgcacgcat ctcagactgg
    46861 gaagcgttga tgatctctgc taccattttc aactgcttgc gaaacatttc tggtgtctcg
    46921 cttttttctg cttgctccgt caatgccgac atcatcatca ttgctgcaaa caattgttgc
    46981 gaaactgaat catgcagctc acgggctaat cgatgtcttt ctaattctaa aatttcttct
    47041 ttggtttgtc catctacata ttggggacgg ctcgttacaa tttgtaattc actagatact
    47101 tcaatcattt tttcttttat cttagtgatt tccttatcaa tatctttgat gtataaatcg
    47161 tccgatgcac ttgggatcgg aagaagcaac aatttggatt cataattgcc agaagaaagt
    47221 gcacgcagct tttcttcgat tttcccgtat tttgtctttt gaataagtga taataataga
    47281 aagacaatta atcctacacc taaggaaatg gctaacaaat ggaagataag cggaacatat
    47341 aataaacgtg cacgcagtaa ttctccccac caatgacttt gattgctggc ataaaaataa
    47401 gtgaaaagtg aaaatagaat aataaggaaa gccgcaatcc ccgaataaac agctagcatc
    47461 gctctggata tttttcccat catattcgta tcacctcgac atctccaatc aaggtattag
    47521 taataatctt caatctacga gggttttcat cataatcact gctgtagatt ttgatttgtt
    47581 catttttcaa agaaaattgc tcttcttcaa acaagacatt tcctactaat gtcgcatgct
    47641 ctaatcggat ggctacgccc aaaggtacta agatccgtgt tctgcctatc ccttttctta
    47701 caatcacaat attatcatct tttggcaaaa gcgtattgcc caaatctatg atagtatcac
    47761 cagaaatgac ggcgatgttg atatcgtccc attcatagac gtcgtttcca atacgttggt
    47821 taccgaataa ttgctgtctt ttccgctcat tattatgtgt ttttaattgc tctgtttgga
    47881 tcatcatgat ctgtttcttt cgccaaaaag catttttggt taaatctatc cctgaaatct
    47941 ctacaccttt cagaccaata aataaaacgg caaatacagc catcatccat aaagcaggac
    48001 tgttgactaa gctaaaaaaa atgaccaaac ttcctaatat caattgaaaa ttttgaaatt
    48061 tagtacgagg atgtttgcgc atagctagat atatattgaa aataccgaat atcactaaaa
    48121 gcaaaagtcc agtattgttt acgatttgcc aaacagcaaa taacagcagt agtgcttcaa
    48181 ctaccacaaa aaaacgccaa gaactattca tcgctctttc tccctcctga cacattctca
    48241 tttcatcata cctatttttt taactgttgg ctataggact ttagatgtaa gatcgtttgt
    48301 tgaaatctaa aatacacaaa gagaaaaaga tctagagtgt atcatcagta agatgaactc
    48361 tctagatctt tcagtcagac tacgcttgtt ctactttgac gattttaact aacatatcgc
    48421 cacctggagt agcaatagtt acttcatcat ttttacgttt gccaatcaat gcttgcgcaa
    48481 taggagaatc attggaaatt ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg
    48541 tgtactcttc ttcttctcca tctggtagtt cgatgaatgt aactgtttta ccaattgata
    48601 cctcatctgc atctacaccg tcgttatcga tgatttgagc aaaacgaatc atattttcaa
    48661 ttgttgtgat tcttccttca acaaatgctt gttcatcttt cgctgattca tattcagagt
    48721 tttcggaaag atctccgaaa ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt
    48781 tgactgtttt tagttcttct aattcttttt ctaatttttc tttaccttct agagtcattg
    48841 gataaacttt ctctaccatg tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta
    48901 atcttttgag gaatatagtc caaaaaagat taccacgctt ttcttcaaat gtaaatgttt
    48961 ttctaaaaat gatcaatgtc tagagcctgt ttttctaaga attatttaca tatttttcca
    49021 ctaattccat atgttcttca taagttttag cgaaataaac atttccagtg ctcgtatctg
    49081 ctacgaaata ataatagtca ttttctgctg gttctaatac tgccttgatt gcctcttcgc
    49141 ttggattgtc aaaaggtcca ggtccatagc cggtattggt atacaaatta tatggtgaat
    49201 ctacagctgt atcttcatac gtcactaatt ctttgtgttc acctaatgcg tataaaacag
    49261 agatatctga ctgaagtggc atatttgctt tgattcggtt aaagaagact tgtgcaatgt
    49321 ttttacgatc attttctttt acgccttctt tttcaactag agaagctaga gttaaaactt
    49381 cttgtacagt caaatttttc tgcttgatag ctgaataata gttttgcatg accgtattcg
    49441 tcttgttcac catttcaatc actagatcct taagcgaagt actcgtgtaa taatcatatg
    49501 tggctgggaa taagtaacct tccaaacggt attttgtgtt ctcagcttta gatgcacttt
    49561 ccaacagttc tgggaatttt tggtgcaact cgttaaagaa agtctcatca ttcactaaat
    49621 ctaaaaattc tttcttgtct ttaccagtta ccttcgctac ccgttcagca atctgatcga
    49681 tatcgtaccc ttctggaatg gcaattttcc catctgcaac ttttgttggc tcagaagtac
    49741 cgccttcttg caactgtttg ccaatctcgt caagagtcat attaggcgct aactgataat
    49801 agccagcttg aaagccagtt aaatttttaa attttgtata ataattgaaa acaatcccgc
    49861 ttttaataat attgtccttc tccaaaatct caccaatctg cttgttagaa gagccactcg
    49921 ggatctcgac ttggactaat tggtcatctg atttatctaa aggcttcaac ccgctatcta
    49981 cataacgata cacggtaaat ccaagaaatc cgccaataat caataatgta agagcaatca
    50041 ctaaaatgat cttcctcaca atcttatctt cttttttacg cagatttctc cgcttgtcga
    50101 ttatatctgc tgtagctgcc ttactggctt cttttttatc tttacttggg tgttgtttgt
    50161 ttcctgacaa aacaactcct ccttactatc aagttcgtaa ctttattcat tatacatgat
    50221 atttgaattg gcgtgaatga ttttatttaa atattctatc aaaaatgtaa gtagtaaatc
    50281 cattactata agattaaaat aaatgttatc aagatacaag ctattttagc tttattcttt
    50341 ctgtaaaaaa gaaaggagac taaataaact attatgaaaa aaggaaaaca tgctaaaaaa
    50401 agaaaatatt catttttaat gatcgttgtc gtgttgttta gccttagtgc gttctctaca
    50461 ggaactattc ttggatggca attttacaat aatgaacact ctcagatagt tgcccatgct
    50521 caatcagctc aaaaaaagtt gatactgact accaatgaac aacagctcgt tgaaaaaatc
    50581 gatcaactat taagaaaaaa tgattatatt ggctcaattt acgtaaagaa aaataatcgc
    50641 atcatcatgg aaaaaggcta tggctatgcg aataaactca cgcaacaacc aaatgatcct
    50701 tctctttatt atcagattgg ttcggttcaa aaagcattga ctactctttt gattttaaaa
    50761 caagtcgagc gtgggcacct ttctttagat actaagctgg ctcaattcta tccacaaatt
    50821 gctggcagcc agcaaattac tatccgtgac ttgctgtata tgcgctcagg cttgcaccga
    50881 actgcttctc caaccatacc gatgactgat gaagaagtcg ttcaatttgc gattcagcac
    50941 ttaaaattta cagattatca gacctctcat tatgattcat tgaactttac catattgact
    51001 ggaattttga tcaaattaac aaatcaaccg tatgaaaaat tattgaccga tgatatcatc
    51061 gaacctttag gattacagca tacaaaattt tatgaccaag tcaaaaattc atccaatcat
    51121 gcactatcct atcaaatgtc taaagaggat gattacttta aaccactgac agaatctgaa
    51181 acagagatcc ataacgaatt agggactggt aatatcagta tgtccgtcta tgatttaaat
    51241 acgctgttta cacaagtttt atctggaaaa ctagtttcca aagacttact cttctcatta
    51301 tggcgtgaaa atgcgaatgg acatccatat agtggtgggg tatatagcgg agataattat
    51361 atcctgtcgc aaggaaatat cgactctttt gatacatctg ctatatttag caaagattca
    51421 caggatgcta tcatcatgga aagcaatata aaagctgaca aaactatcaa actgcctact
    51481 gctgatctaa gaaaccaaat ctatgacctg atggaaggaa ctactaagtt ggcaaatagc
    51541 tgatatgatg aaaagatagc aaatactact tctttgaata gcatttgctt aaaaaaagta
    51601 ctcgactaaa cgtaggaaag agtgtgggac aaatctaaaa atcaattttt gctacagact
    51661 ttaattccaa ataaacagaa cagaaagagg tgttcttctt tctgttctgt ccaaattatt
    51721 ctaagatttc tctttaccaa cattatttga caaagggcct attaaatcta aaatagtatc
    51781 tttagattgc taaaatatgt gctagcaaat cattcatggc ttgttcttat ttttaatttt
    51841 atgcaataaa aaagaagaac aattagcgtg attatagaaa taaataatga gattttagaa
    51901 aaaaatgtgt atgaatacct gattgttact ttatttattc cactttcact ttttactgta
    51961 acaaaaggac ctccttgttt tagtactgta gcattttttg aattaacagt aaccgttaat
    52021 cctttatacc ggaatacagg taaaactatt gttttgcctt ttgaagattt aaacgtaaat
    52081 gtaatttcat ctttatttgg agaaaagcta ctataaataa cttgaccatc ttctgtaact
    52141 tgatggtttt tcaaaagcaa ttgatctttt ttatagccta taggtacata atcaaaattc
    52201 tcaaattctt caagtttatt ttccatttgt tcattagaat aagttccttg aaaattc
//
LOCUS       DAYYCQ010000009        51575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000009 DAYYCQ010000000
VERSION     DAYYCQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.009"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..589
                     /locus_tag="ACHAYF_000575"
     CDS             47..589
                     /locus_tag="ACHAYF_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8578040.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(672..1223)
                     /gene="lepB"
                     /locus_tag="ACHAYF_000576"
     CDS             complement(672..1223)
                     /gene="lepB"
                     /locus_tag="ACHAYF_000576"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8578041.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1375..1602)
                     /locus_tag="ACHAYF_000577"
     CDS             complement(1375..1602)
                     /locus_tag="ACHAYF_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8578042.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(1599..2117)
                     /gene="msrA"
                     /locus_tag="ACHAYF_000578"
     CDS             complement(1599..2117)
                     /gene="msrA"
                     /locus_tag="ACHAYF_000578"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8578043.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2137..2850)
                     /locus_tag="ACHAYF_000579"
     CDS             complement(2137..2850)
                     /locus_tag="ACHAYF_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8578044.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(2853..3749)
                     /locus_tag="ACHAYF_000580"
     CDS             complement(2853..3749)
                     /locus_tag="ACHAYF_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8578045.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(3907..4749)
                     /locus_tag="ACHAYF_000581"
     CDS             complement(3907..4749)
                     /locus_tag="ACHAYF_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8578046.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            4881..5534
                     /locus_tag="ACHAYF_000582"
     CDS             4881..5534
                     /locus_tag="ACHAYF_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8578047.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(5597..6124)
                     /locus_tag="ACHAYF_000583"
     CDS             complement(5597..6124)
                     /locus_tag="ACHAYF_000583"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8578048.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6144..7091)
                     /locus_tag="ACHAYF_000584"
     CDS             complement(6144..7091)
                     /locus_tag="ACHAYF_000584"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8578049.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7103..8986)
                     /locus_tag="ACHAYF_000585"
     CDS             complement(7103..8986)
                     /locus_tag="ACHAYF_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8578050.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(9149..11227)
                     /locus_tag="ACHAYF_000586"
     CDS             complement(9149..11227)
                     /locus_tag="ACHAYF_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8578051.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11240..11515)
                     /locus_tag="ACHAYF_000587"
     CDS             complement(11240..11515)
                     /locus_tag="ACHAYF_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578052.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(11877..12332)
                     /locus_tag="ACHAYF_000588"
     CDS             complement(11877..12332)
                     /locus_tag="ACHAYF_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578053.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(12486..13694)
                     /locus_tag="ACHAYF_000589"
     CDS             complement(12486..13694)
                     /locus_tag="ACHAYF_000589"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8578054.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(13806..14138)
                     /locus_tag="ACHAYF_000590"
     CDS             complement(13806..14138)
                     /locus_tag="ACHAYF_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8578055.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14286..15161
                     /locus_tag="ACHAYF_000591"
     CDS             14286..15161
                     /locus_tag="ACHAYF_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8578056.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15243..15824)
                     /locus_tag="ACHAYF_000592"
     CDS             complement(15243..15824)
                     /locus_tag="ACHAYF_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8578057.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(15830..17089)
                     /locus_tag="ACHAYF_000593"
     CDS             complement(15830..17089)
                     /locus_tag="ACHAYF_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8578058.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17091..18137)
                     /locus_tag="ACHAYF_000594"
     CDS             complement(17091..18137)
                     /locus_tag="ACHAYF_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578059.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18372..18647)
                     /locus_tag="ACHAYF_000595"
     CDS             complement(18372..18647)
                     /locus_tag="ACHAYF_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8578060.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(18890..20200)
                     /gene="der"
                     /locus_tag="ACHAYF_000596"
     CDS             complement(18890..20200)
                     /gene="der"
                     /locus_tag="ACHAYF_000596"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8578061.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(20382..21611)
                     /gene="rpsA"
                     /locus_tag="ACHAYF_000597"
     CDS             complement(20382..21611)
                     /gene="rpsA"
                     /locus_tag="ACHAYF_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8578062.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(21746..22426)
                     /gene="cmk"
                     /locus_tag="ACHAYF_000598"
     CDS             complement(21746..22426)
                     /gene="cmk"
                     /locus_tag="ACHAYF_000598"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8578063.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(22475..23110)
                     /locus_tag="ACHAYF_000599"
     CDS             complement(22475..23110)
                     /locus_tag="ACHAYF_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8578064.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23176..24579)
                     /locus_tag="ACHAYF_000600"
     CDS             complement(23176..24579)
                     /locus_tag="ACHAYF_000600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8578065.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(24566..25597)
                     /locus_tag="ACHAYF_000601"
     CDS             complement(24566..25597)
                     /locus_tag="ACHAYF_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578066.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            25628..25849
                     /locus_tag="ACHAYF_000602"
     CDS             25628..25849
                     /locus_tag="ACHAYF_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8578067.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26014..26751)
                     /locus_tag="ACHAYF_000603"
     CDS             complement(26014..26751)
                     /locus_tag="ACHAYF_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8578068.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(26904..28244)
                     /locus_tag="ACHAYF_000604"
     CDS             complement(26904..28244)
                     /locus_tag="ACHAYF_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8578069.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(28481..30004)
                     /locus_tag="ACHAYF_000605"
     CDS             complement(28481..30004)
                     /locus_tag="ACHAYF_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8578070.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(30382..30993)
                     /locus_tag="ACHAYF_000606"
     CDS             complement(30382..30993)
                     /locus_tag="ACHAYF_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8578071.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31116..31241)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(31323..32039)
                     /locus_tag="ACHAYF_000607"
     CDS             complement(31323..32039)
                     /locus_tag="ACHAYF_000607"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8578072.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32045..32614)
                     /gene="scpB"
                     /locus_tag="ACHAYF_000608"
     CDS             complement(32045..32614)
                     /gene="scpB"
                     /locus_tag="ACHAYF_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8578073.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(32598..33368)
                     /locus_tag="ACHAYF_000609"
     CDS             complement(32598..33368)
                     /locus_tag="ACHAYF_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8578074.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(33365..33736)
                     /locus_tag="ACHAYF_000610"
     CDS             complement(33365..33736)
                     /locus_tag="ACHAYF_000610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8578075.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(33755..34642)
                     /gene="xerD"
                     /locus_tag="ACHAYF_000611"
     CDS             complement(33755..34642)
                     /gene="xerD"
                     /locus_tag="ACHAYF_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8578076.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(34738..35211)
                     /locus_tag="ACHAYF_000612"
     CDS             complement(34738..35211)
                     /locus_tag="ACHAYF_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8578077.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(35316..36179)
                     /locus_tag="ACHAYF_000613"
     CDS             complement(35316..36179)
                     /locus_tag="ACHAYF_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8578078.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(36375..38474)
                     /locus_tag="ACHAYF_000614"
     CDS             complement(36375..38474)
                     /locus_tag="ACHAYF_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8578079.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(38694..39803)
                     /gene="rpoD"
                     /locus_tag="ACHAYF_000615"
     CDS             complement(38694..39803)
                     /gene="rpoD"
                     /locus_tag="ACHAYF_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8578080.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(39827..41668)
                     /gene="dnaG"
                     /locus_tag="ACHAYF_000616"
     CDS             complement(39827..41668)
                     /gene="dnaG"
                     /locus_tag="ACHAYF_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8578081.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(41850..44165)
                     /locus_tag="ACHAYF_000617"
     CDS             complement(41850..44165)
                     /locus_tag="ACHAYF_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8578082.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(44350..45210)
                     /locus_tag="ACHAYF_000618"
     CDS             complement(44350..45210)
                     /locus_tag="ACHAYF_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8578083.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(45269..45901)
                     /locus_tag="ACHAYF_000619"
                     /pseudo
     CDS             complement(45269..45901)
                     /locus_tag="ACHAYF_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            46068..46829
                     /locus_tag="ACHAYF_000620"
     CDS             46068..46829
                     /locus_tag="ACHAYF_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8578084.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            46855..49446
                     /gene="mprF"
                     /locus_tag="ACHAYF_000621"
     CDS             46855..49446
                     /gene="mprF"
                     /locus_tag="ACHAYF_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8578085.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(49634..50821)
                     /gene="tuf"
                     /locus_tag="ACHAYF_000622"
     CDS             complement(49634..50821)
                     /gene="tuf"
                     /locus_tag="ACHAYF_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8578086.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            51189..51545
                     /locus_tag="ACHAYF_000623"
     CDS             51189..51545
                     /locus_tag="ACHAYF_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8578087.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        14330 a        10806 c         8842 g        17597 t
ORIGIN      
        1 attttatggt atccttttat tattaactga caaaggagag aatattttga acactttgat
       61 tgcaaaagac gtttttagat tgcccgaaat gaatgcaaga ttgttgtcga cctctgctct
      121 tttattagcc cttgatgtca tcttatacaa gatagcgatt ggtcctgctt attttcaaat
      181 cacattcgga tttttaagta tggcgcttat gggttatcta tatggaccga tctgggctgg
      241 tttgcttgaa atgttgtcga acactttaaa tttcacgata tttggttcag gaacgtttca
      301 agctgctttt cttatcacgg ctttccttgg tggatttatt aatggcttat ttttatatca
      361 taaaaagata aacctgcctt ccgtcatcgc tgcccaatta ttgattatga tcccagtcag
      421 cctttttatc aattcttggc tgatgtctgt tttgtttggt gcagatttca aagtaatttt
      481 tctaggtaga ttcatcagga atatgatcca aatccctatt caaataatgg ttgtttattc
      541 atttatcaaa actttagagg ctagaaaagt gtttaaacga gctgtttgat tttacgtact
      601 aaggtagtaa actgattttg gattaaacaa taaagcgtgc agtctcaaca gactgcacgc
      661 tttattgttt ctcactggat ccattttatt tcatcaagtg gataatagac aaatcttgct
      721 ttacctagaa tatcagtttt attgatcgtg ccaaacgaac gactatcttt tgaaactctt
      781 cgattatctc caagtacaaa ataattatct tccggaagct tggattgtcc cgttagctct
      841 tgtagtgtaa agtccgtcgt ataggatgca tttgcatgat ctgatttcag gtttttagtt
      901 agataaggtt ccttaactac tttcccattg atcttcaatt gatcattttg gtagctgaca
      961 ttttctcctg gaagaccgat tactcgtttg atataaatcg tgccatctgc taattgaaaa
     1021 acaacaatat caaatcgtct gatctccgaa aatttttcca tcacaaccat atcaccttgc
     1081 ttcaacacgt tttccattga attgccttcc actggtaccg ggatgaataa aaatccccgc
     1141 aacatgaacg caaggaatac agaaacaagt aagtatttga atacaagcca gaagcgatta
     1201 atatagcgtt gtttttttgt caatctctat caatccatcc ctcttttttt cattctttct
     1261 ttattataag aaaaatatca aaaaaatgcg actagatagc tgaaataaaa ataattcttt
     1321 caattaggct attggacaaa aaactttttt gatcataaag tacaaaacta tctcttatat
     1381 gtcatgatta ttttctaaat atttttccca tgcttcgtca aagatatcca tggacggcaa
     1441 atagtcgaca ttcagttcca gataattaga aatttcttcg tagctagagc tgtgcttagg
     1501 aaattggatg tctttaccgg cttctgtagc gaaaacagat aaagcatcat gtgtgccacc
     1561 gcctcgcagt gtcattaaat aatgaaaaaa gcttcgattc acccttcgtt cctccaattt
     1621 tcttcgataa aggcagcccg gcgtgcatgg tttcttttaa aattttcagg atgcttttta
     1681 taatactctt gatggtcttc ttctgctaga taaaatggtt gggctggttc gatttttgta
     1741 acgattggct gttcgaaacg tccactttca gctaactgtt tcttactttt ttcagcaatg
     1801 atccgctgtt cttctgtcaa ataaaagatc accgggcgat aattgtcacc gcgatcctcg
     1861 aattgtccga aagcgtcagt tggatcagtt tgctgccagt aaagttctac taattgttca
     1921 tatgaaatca atgttggatc atagataatc tccactgctt ctgtatgtcc agtcgtttgg
     1981 ctttttactt gttcataagt cggatctggt acatgccctc cagtatatcc tgacatcact
     2041 gtatggatac caggaagcgt gtcgaaaggc tggatcatac accagaagca gcctcctgca
     2101 aatattgctc gttccattat gattccccct ctaatactac tcatccgatg attttttatt
     2161 ttcttttgtc ttgctttcag tactgctggt gctagttttg ttggtatttg agttgtttga
     2221 agcatcagag gtatcttttg gcaaatatag actaaaccga atgtcatcat ttactaagtc
     2281 gattttatct gctttaatat acatcccgtt ttgcattcgg aattgatcca acctaagcat
     2341 gatagtcaga tcatctgtat tgacttcgat ccaatctggt aatttatagt ttctcttgat
     2401 catattcatc acatctttga ttggcaaact caatgttccg atagaaagac ttctagcttt
     2461 tagttggaca tttccgtttt ccatcacata cggatcaaaa tataaataga aatcgatagg
     2521 aaaacctagt acttcgaaag taccattgag tagtgcttca ttttccaaat aaaatttata
     2581 ttcaatatca ctatcttttt gatactcact aaggaaaaag ctgatgattt gattgacttt
     2641 ttctttattt gaattgatcg ttaagacagg atctccttgt ctttccgtaa gagctggtat
     2701 ttctgggagt tctggttccc gattagcaaa aatacggctg cctaaaaata cggtcgaacc
     2761 aagtactacc ccaagaagga taaggaaggc gaatttccag taatttgttt tctttgtttt
     2821 gtttgaagat acgtcagatc ttgttttcat gtttattgat catcctcctt ctctaaccat
     2881 tggttttgtg tctcgatcag ctgatctctc actgcatttg ccatcaattg ataacctaaa
     2941 ttatttggat ggaaatgatc ttcttcatag agcacattat ttttgatgcc atttagattt
     3001 tctgtcgtgc tagttgtttg atctgttcct tcttctgtga cacctacttc ctgattcaat
     3061 ccttgataaa gcaaatcatt gattgggata aagtagcagt tcggcatttt ttttgtcacc
     3121 gtttctgttc ctttgttcca attgttaaca attgtctgca tgtcagtgat ctcaggaaag
     3181 ttcaaataaa atggattgta gatgcctaaa atgtaaatag gtgcggtttt attgagggat
     3241 cgaaattctt ccaatagttc tgtgacattt tcctgatagt ttttcagtgg ctttttaaag
     3301 gaattgaccg ttaacccgaa taaattattt tggataactt tcatcaaatc attaccgcca
     3361 acagtcagtg tgataaaatc agctgttttg atattgtttc gaatttcttc ttttgttttt
     3421 actcgcttta agatctggtc acttcgttca ccatttacac catagttttc tgtttcaacc
     3481 gcggtcaaat tataacggtc ttttagatca tttgcgacaa ggggaacgaa tcctccttgc
     3541 ttggtttgat ctcctactcc ttcagtcagc gaatctccga tagctatgta gtgaagtttt
     3601 tcttttttat ttggatcttc tctcacagta gttactggtt tcattagtgg gccagctttc
     3661 ggtatagaag cagctaaacc tgcatagatg atgatcgcaa ttaataatgg gaacaaaacg
     3721 aggatcaaat gtcgtttcgt taatttcatt ttttcacctc ttgtattcga ttggttctaa
     3781 tgttatcaaa aattttatca aaaaggtagt ctttatgaaa aaagaaaaca attattttcc
     3841 taattttata aagaaaaaga gaataagctg caagcctttc aggctgaggg cttatcctct
     3901 tttcgactaa tctacataat acatgattgc aaacgcgtct ttaccagtat gcgtagcgat
     3961 aaccggattc gtatgcagta cagggatatg catgtccggg aataattctt gcaaaccctc
     4021 tttgaattct tgcgccaatt cttttgcatc cgcatgtgaa ataccgattt gtcgcacatt
     4081 tggcaattct ttcagttcat ttttcaaatt attaaaccat ttatcgaacg ttttctttcc
     4141 tcgcccctta gttacaggga ttaattcaga atgggcgaaa ttcatcacca cgcgtatgtt
     4201 caaaacacta gacagcaaac cagttgttcg gctgattctt ccacctttta ccaagttatc
     4261 tagtgtggat attccgatgt ataattttgt attgtcttgt acttttttga cttcttcaag
     4321 gattttctct actgttttac cttcttttgc taattcagct gctcgaatta cctgaaaaga
     4381 taatccttgg tcagtggtat cactgtcgat aaccgttact ttcgtttttg ttagattact
     4441 ggcttgtctt gctgcttcta ctgttccact caagcctttt gtcatatgga tcgaaatcac
     4501 ttcactgccg tcttctccta gctcatcata catcgagatg aattctccaa ttggtggttg
     4561 gcttgttttg ggcagatttt ttgctttcgc catcatttcc ataaatcttt cgccgtctag
     4621 ttggtcatca tcgggatata ctgttccgtc gatcatgatg gatagcggca tgacacgaat
     4681 gcctaaagca tcgcgtacat ttttttccat cgtacatgaa gaatctgtta cgatttttac
     4741 atttgtcatt attttctctc tctctttctt taacaagatg ccttattcat gttaaactac
     4801 atgtaagaca atttctattt ttagtataac agacaaataa tgatttttca ttaattacac
     4861 ctagtaaagg agccgttttc atggaaaaaa cgaaatttaa tcgaacgtat gaaatattaa
     4921 atgaagtgtt taatgcagtc acccatggaa ttgggactgg attgagtatc gcgggactag
     4981 tggttctttt agtaaaaggt gcccacttgc attcctccct ccatattgtc tcttatgcta
     5041 tctatggatc gatgatgatt cttttatttt taacgtctac tttgtttcat tcattgattt
     5101 ttacaaaagc aaaaaaggtg ttccaagtat tcgatcatag ttcgattttt cttttgattg
     5161 caggaagtta tactcctttt tgtttgctga gtatccgagg atggctaggt tggactttat
     5221 ttgtattaat ctggttgttt gcgattcttg gcattatata taaagcgttg acgttgcata
     5281 aacgggagag agtatcaaaa gtatctacga tcatctatat ttttatgggg tggctctgtg
     5341 tcgtggctgc tgttcctctt tatcactctc ttggaatgac tggtgttatt ttgatggtag
     5401 caggcgggct ttcttacacg ataggcgcat tcttttatag cttgaattct atccgctata
     5461 tgcatgttgt ttggcatttg tttgttatgt taggtgcagg ttttatgtac ttctctgttt
     5521 tattctttac ttaataaaaa aaataaagct atggatggga cagctctaaa taacagctag
     5581 tctatccata gcttttttat atcgtcaatt cgcctgtccc gctttttaca ggattctcat
     5641 tacgctgata cgtttcaaat tgatgcggat aacgattttt ttcatccatt tctccttgac
     5701 tagtattgat cagtgaccaa tcagaccagt caacaggtgg aaaataagca tctccatcga
     5761 atacatgatg gatgactgta cggtataata tcgtacaaaa tggcagaaat tcttgataaa
     5821 cagctgaccc tccagcgatg aaaaagatac cgtctgtttc tttttccaga gcaagcacgt
     5881 cttccacact atgcatgaca tgcacgcctt ctgctttata ggaagaatct ctcgtcaaaa
     5941 caatcgtctg acgattcggc aacggacggc tacccatccc ttcgaatgtt tttcgtccca
     6001 tcactagtgt atttgcttct gtcatttgtt tgaagaattt cagatcattt ggcaaacgcc
     6061 aaggaagacg gtcttctttt ccaatcaatc cgttttcatc ctgtgcccaa attgcagcta
     6121 acattttctc atcctttctg attctaaaca gcgataggag ctttgatcgc cggatgtggg
     6181 tcatatcctt ctaattggat atcttccatc tcaaaatcaa atactgactg cttttcctga
     6241 ttcaaaacta aagtcggaaa tgcgcgtatt tctcggctca attgttcttt catttgttcg
     6301 atatgattga tataaagatg agcatctcct aatgtgtgaa caaattcgcc aaccgctaaa
     6361 cctgtttcat gtgcaattag gtgagtcagc aacgcataac ttgcaatgtt gaacggaacg
     6421 cctagaaaca catccccact gcgttgatac aattggcagc ttagccgtcc ctcttgaaca
     6481 taaaattgaa acatcgtatg acaaggaggc aaagccatgc taggaacatc ttccggattc
     6541 catgcagaaa cgatcaatct tctagaatca ggagtctttt tgattgcttc gatcacttgt
     6601 ttgatttgat cgataaagcc accgtctttt gtttcccagt gacgccattg atagccgtag
     6661 atgtgtccaa gatcgccgta tgtttcagca aaggaatcat cggttaggat cttctcgcaa
     6721 aaaagcttgc tctgcttttt atattcttca ttgaattcag gatctgtcaa acatctatgg
     6781 ccaaaatcag tcatatctgg tccattatag tctggacttt tgatgtagcg ttcaaatgcc
     6841 cattcatccc aaatatggtt tttattttgt aatagatagc ggatattcgt atctcctttt
     6901 aaaaaccaaa gcaattcgct tttgatcaat ccaaaaggta ctcgtttcgt tgttaacaaa
     6961 ggaaaccctt tttgcagatc aaaccgcatt tggtatccga ataagcttaa tgtgcctgtg
     7021 cctgttctgt cttctttacg atgcccttct tccaacaatt ttcttcctaa gtcaagatac
     7081 gcttgttcca tcctttttcc tcctaatctt ctacccattc actcaagtat tcccaccgtg
     7141 ccattttttc ttcaagttgt gcttctgttt cagatacgtc gttttgcaat tcttgtaatc
     7201 gagtgaaatt atctccttga tgattcatct cttcttggag caatgagatt ttttcttcaa
     7261 gttctgcaat ctcatcttca atcgtttccc attctttttg ttccatgtaa gataatttct
     7321 tcttttcagc aggttccttg ctctgtattt ttggtttcgc tttttcagca ggctgctggg
     7381 tctctttttg cttcagtaaa taactggaca tgtttccgta ataaagttct tgttttccgt
     7441 ttccttggaa tactaataac ttatctgcta ccttgtccaa gaaataacgg tcatgggaaa
     7501 cagtgatcac agcacctgga aaacttctaa aataatcttc caaaatcgtc aatgttgcta
     7561 tgtccaaatc attggtcggt tcatccaata ataaaacatt aggttgctga atcagcagtt
     7621 tcaacaaaaa taaccggcgt ttttctccgc ctgaaagttt gcctatcagt gtcccatgca
     7681 taaatcttgg aaataaaaat cgttcaagta attctgctac tccgatttga ctgccatctg
     7741 ctgtttttgc ttcttcagca gcttcttgta agtaagcaat catccgttta tctggtgcca
     7801 tttcttcatt ttcttgtgta taatacgcaa gatgaaccgt ttcaccgata gacattgtac
     7861 cgctatccag gggaattctg ccagctagaa tattaagcaa tgtcgatttt cccgcaccat
     7921 ttttaccagt aatacctagt ctttcccgag attgaatcaa taaatctaaa tgttctagca
     7981 gtgtttggtc atttatacgg taactgccat ctttgatttc cagtactttt ttccctaaac
     8041 gttgtgtcgc taggttcagc tctaaatcat cttcttggtt tacttggtac aagttctcct
     8101 ttagatcttc gaaacggttg atccgtgctt gctgcttggt gccacgtgcc tgtacgccag
     8161 ccctcatcca agctaattcc tgtttataaa gccgcttgcg cttttcttct tgttctactt
     8221 ctacacgatc acgttctgct ttttctaaga cgtaagcttc ataatttcct ttatactcgt
     8281 ataattttcc aaatgaaagt tcgaagattc ggtttgctac tctatctaga aaataacgat
     8341 cgtgggtaac catcaatagt gcgccacggt attgtttcaa atagttttcc aaccactcga
     8401 tcgcttcata atctaaatgg tttgttggtt cgtctagtaa tagtaaatcg ggttcatcaa
     8461 tcaacacttg cgccagacta actcgttttt tctgaccacc tgataaagta ctgatttttt
     8521 tatctaattc actgattcct aatttttgca aaataatctt tgcgttggta tctgtagtcc
     8581 aagcatcttc tttattcatt tgttcttctg ctttagcata acgtttttgc acatcagggt
     8641 cttctccgtt ttcccctagt tcgatcaatg cttcctcata tgctcgaaca gtttggatca
     8701 aggggctgtt tccttgaaag actgcttgga gaacagttaa ttcatcggaa aattcagctg
     8761 cttgagaaag ataaccaata cggtaatcag ttggataaaa cactgtttgc cgatcaccgt
     8821 cgctgctatc gataccggca ataatattta agagacttgt tttacccgtc ccgttcgtcc
     8881 caatcaaccc gatacggtct ttttcgtgga ccaaaaaaga aatctggtca aacaaatctt
     8941 tttcaccata agtttttttg aggtcagtta cttttaattc tttcaacatg ttccctcctt
     9001 atcttggata ttgtatcata aaaatagtga acttcaggaa aatttttttg gtatttgttc
     9061 tttttgtgtt ctcaaaaaaa gaacaggacc gattcgatcc tgttctgata tgttttgttt
     9121 tttataaaac ttgaaaattt ttaccttttt attttattgg actcgttttt tttgactttg
     9181 ctcgaaacgc aaatcggaaa gaaaagttat cagatggttt gttcatcgta taagattcta
     9241 acaaattgat attcctgcga tagctgttcg cagaaaacgt cgtgattttt tcagcaactt
     9301 cgtattcatc aatgattttt ctttcaagat aataacctct tagcattttt ttcgtaaaca
     9361 gactatcttg ctggatcatc cacgaagaaa tcaaattccc cgagttcatt tttattcctt
     9421 caagtttgcg ttgctggata aaaaattgaa ccatcgtttc atcataaata tcagtcaagt
     9481 tatctaaact ttcaataatc aactgagtat ttttttgata tgtctctctg atttcttgga
     9541 tatcttcatc actaataaat gtctgacgat cagcatatct tctttgccaa aacattttcg
     9601 gatggagaac gattcgcatt aacctacgga caacaatgaa ccaaaagccg ataaaagaca
     9661 aaatctggct tgtgatagaa tgttcgaatc gagaaagaaa acgggagtaa aaacgataac
     9721 cattagaaga aagcttgccc gaacgataac tttcatccaa tccatcccgt tcgatcgaca
     9781 aaatcagtgc ttgtatttct tgtacttctt gcttttcatc atcggttaaa tcttccaagt
     9841 acagatcacg tatccgttct tgataatttt ctatcactgc ttcagtggct aaaaattctt
     9901 ttgtattacg atcaatttcc tttatttctt ttcgcaaaac ttcaacgacc tcttctaaaa
     9961 tcatcaaagc attcaaatcc gtgcttttcg cttcctctgt ttccgtcagc ataggcaaga
    10021 caatgatccc tatgaccaaa gtcactaaga tgacacccgc cgtcagaaac aaaagcaaag
    10081 accgctggta aaacatcata ttattgattg aaaatggcaa aataaaaatc gtagccaaac
    10141 tgactgttcc ctttacaccg ccaaatgtta agatcaagat ctcgttcaac tgttttttga
    10201 actttttact gccatctttg aatacataaa aaagactgat agaaatgaat ctgatcacaa
    10261 aaagcatgac agagattaat acgataatcg ctaataaaag accgtttgga tataaaccgt
    10321 ctccccatac tggcgagaac acttgcgtca attcgattcc taaaaatata aatactaatg
    10381 cattcagtgt aaaagcaata gttgtccatg tactgtggga caagctcgac aattctgcat
    10441 caaacacaga gattttccta aaaccagaag cttggagaat acctgctgcc actgcagcaa
    10501 tgattccaga tacatcaaat gcctctgcca atacataggc tagaaaagga agcaaaagtt
    10561 cgatcagcag ataccctgtg acatcttgtg ctgatgcttt ttcgatcaaa tgaattattt
    10621 tttgtttgaa ccagacaagc aaaaagccga tcaaagctcc cccaatacta gaaactacta
    10681 gactcatccc cgcgtttact gctgaaaaag atccggtgat cagcgcacca agagcaaact
    10741 ggaaggcagt aacaccagat gcatcattca aaagaccttc tccttccaaa atatgcatcg
    10801 cctttttagg gatattcact cgtccagaaa gagaactgac tgctaccgca tctgtcggac
    10861 ctaacgctgc tccaaaagca aagcaggcag caagtgggac acttggcaac aggaatgata
    10921 acgtcgcacc taccccgaca agggtcaaaa tgactcctcc aaatgccaag aacaaaattg
    10981 tcccgaaatt tttgagaatc gaagaaatat ctgctttttc tccttctcta aataacaatg
    11041 gtgcaataat cataactaag aacaattcag gttcaaaatc gattgattgt ccccactctg
    11101 tcagacctaa taccactcca aggaaaattt ggatcagtgg agctggaatc attggcaaaa
    11161 tgcggctagc tatgttcgat aaggtgatcg ttactgcaaa tacaataact aactcagcaa
    11221 attccatcgt ccatcccctt tattttttat tttgtgtaag catttttaaa aattgttctt
    11281 ttttctttaa aattttggtt tcgactactt caacctgtgt ttggttcccc tctaattcac
    11341 actgtacacg ttttgactcc aaacgaagca gatcatgttt cagacgttcc aactgacgat
    11401 acatcaatat atttctttta cgcaacttac aaagtatttg acggcggtca tccatcgaaa
    11461 gggcgccata gcgatcatgc aacagttttt tttgttgttc aaaactctct ctcattttcc
    11521 agatctccta tacacaaact tccatcatta tagcaaagtt ctttttggat gaaaaatatt
    11581 tcattgtttc atcgtttatt gctagttaga aatttccata aatttttcaa cgcctcattc
    11641 tattccaaat agtatcagat atcaagtaaa acttaagaaa gataaaagtt tctgacaaaa
    11701 ggatgtttac tttcccattc ctgcctttct tgttctttta tcacaacaaa aaaatggggc
    11761 tgcgacaaaa atcttgtcac agtcccttat tctgaataaa cggtgtggaa gagacagcct
    11821 atcagtagta ataagacaca tctctttcac agcctcattt ttgcgtaaaa atcgatttat
    11881 tcgaaaaatt ccccgtatac atcttctaca cgttgatctg gattgatcac gatatacaag
    11941 ttacctgttt tttcgctgat ttttgttgat tgatattgat tgtctaatcc ttctgttcct
    12001 ttatctagat atgggaagta cacaagatca ggtgtgccat ttcggtaaag gatttccatt
    12061 cgctcgatat cttcgtattt caatgcgcga ttaaacatgc ccatttccag tccgcctagg
    12121 ttgatatcat gtgtcgcaac tgcgtcccct tcatggaaga tttcgatttt aaatccagca
    12181 cacggatgga tttcaacaaa ttcactgcca ttgattcgtc caaaacttgt tgtgatttgt
    12241 ttgatccaaa gatcgccgat atgacggcgg ttgatcgtcc aggtttctcc atttctaaaa
    12301 taaaatttta gcgctgtcat ttctttactc attaaaaaga ctcctttatg atgtaattta
    12361 ttcttgtact acctcagttt agcacaaatt tatgtaagtg gtcacatctt atgttttttc
    12421 ttctaatgac gtatctgccc actttaagta gccaacaaag ctgagcagat acagtctagt
    12481 gttttttaaa tcaatttttc tttcgataaa aaatccaaaa cctgtttttt atcatttttt
    12541 attctttgtt ctacgacggc tttttcagct aattgcaaag cttcactaac ccacttacca
    12601 ggtttttttc cagtttcttc tagcaagatt ttaccatcta ctgctaattc gcttaatgac
    12661 ttgataggta aacgatcata ggattctcct acggcatcca tctgaggtgt ttcgtcaaaa
    12721 tagatcatca attcttctac ttgcagtgca tgttttttgc ctaagaagta caattcgaat
    12781 ggttccaaag ctccagtttc tcgttgtcgt agtccttgga caagtgcttg aacgtcttta
    12841 atcatttggt tagaacactt ccacgatttc aaaaaggaac gaatctcatt ttcttttaga
    12901 tccagttggc tgatcaataa tgcccatgct tggctctctt ctttcaatgg gacggatggt
    12961 aattcagcaa aacgtaaaag tgcttcgcct tcttctttca aaccgggaca gcaaatgtag
    13021 cactctgttt caataaatgc atggattccc gcggcccgtt catttcccaa gagcatcttg
    13081 atgaattcca catttatacg ttctacagat attttaccta atagctcgtg gttttctttg
    13141 atggcgtaga aggtttcttc ctctaatttg aaccctagtt gactgacaaa acgcaacccg
    13201 cgcatcattc gtaaggcatc ttcatggaac cgctcatgag gattgcctac agctcgcaag
    13261 atctgttctt ctagatcttg cagaccatcg aacaaatcga tgatttctcc atcttctttc
    13321 aaagcaaacg cattgatcgt aaaatctctt cttttcaaat cttctgccaa cgaacgaacg
    13381 aattcgacat gatctggtct acggtagtcc tgataagttg attccgtccg aaatgtggtg
    13441 atctcatatt gttcatcatt ttctaatacc aacacagttc catgttcgat cccaatatct
    13501 atcgtttttg gaaaaagctg tttgatttct tctggaaatg cgcttgttgc gatatccaca
    13561 tcatggattg gctggttcag caagatatca cgaacacttc cacctacaaa ataagcttca
    13621 tatcctgcat tctctaattt tttcagaact ggaattgcct tttggtattc cagaggtaat
    13681 tgttttaatt tcatttttcc aacctttcct gcgtgtttct tgtcctgaga taaagactca
    13741 gttgcatggt gactgtttga caagtccgtt tcagagtctt caaggccagg accacttcat
    13801 cttgtttact cgtccttacg ctcaaagcga ttatggtctc tttgattgaa tttttccatg
    13861 tttttttcaa aaacttcagt taagtcgata ttcaatgaat tagccatgat catcgtaacg
    13921 aataaaacat caccaagttc ttctgctacc gagttaactg gttcagaagc ctttttgctt
    13981 ttttctccat aagtgtggtt gacttctctt gccaattcac ctacttcttc agttaatcga
    14041 gccatctgag ctaatggaga gaaatacccc accttgaatt gctggatata ttcatccact
    14101 tcctgctgca tactgtataa agaacgcttt ttatccatct ttttccctcc aaaactatct
    14161 taccaaaact ttaacgcaaa tcctagataa gagcaattga gaaaatcgaa tttccatgct
    14221 acatttagga aggaatttct aaatggaaat tctgtgaaaa tacataattg aaagctggaa
    14281 ttcatatggt caaacgaaaa acagtaaaag aagttctttt tatcattata ggaacaagta
    14341 tttatgcatt tggtcttgtt tacttgaaca tcgctaatca tttagcagaa gggggagtct
    14401 cagggatcac cttgatctta agagctttat ttggtattga tccagcttat acgactttat
    14461 taatcaacat cccattgatt ttactaggag gaaagatcct cggtaaacgt tccttggcct
    14521 atactgttct aggtacactt tctttgtcat tctttttatg gatctggcaa agggttccac
    14581 tagaaatcaa tttacagcat gatctactga tcgtttctct acttgcgggt ttagttgcag
    14641 gtgtaggaag cgggatcgtc taccgcatgg gtggaacaac aggcggcagt gatattatcg
    14701 ctcggatttt agaacaaaat tacggcatca ctatggggcg ttccctattg gcatttgata
    14761 tcatcgtctt acttgcttca ttaacctata tcgacttgaa acgtatgatg tatacgttga
    14821 ttgtttcgta tgttttcagt cgtgtgattg attcgatctt agatggcgga tattctgcaa
    14881 aaggtctact tgtcgtctcc aacaaaagcg aagagattgc accactactg atgaccggtc
    14941 tgcaaagagg tgtgactttc ctttctggag aaggcgcatt ctctggtaat tcaaaaaaaa
    15001 tcatctatat ggtcgttagc tctagtgaat taactgaagc taagaggatc gtccacgaga
    15061 ttgacgaaaa agctttcctt tcaatcatca atgtgcatga agtggaaggg caaggcttta
    15121 cttatttgaa gccacagaca agattactga aaaaactata agaaaaaaga caaagatagg
    15181 ctatgaggaa agattccaca tccagctgac ctttgtcttt tttagtacta tactattttt
    15241 aactaattgt tatgttcact tacccggtta cttttctttt gtgtctgcaa aaggatcttt
    15301 cggttaactt catcagataa tttcaaaaaa gcatccctat ctccatcttc tagtgcttga
    15361 tcgatttgag cataaagcat ttttagtttt gcctcttctt ctttttcttg aaaatatcgt
    15421 tcaattaatt cagtatcttc ttcactgact gaagaatccc aagaagaaaa tggattgtct
    15481 tctaagatag atagatattc tcttgtctgc caagcatttt cgaatataca ttccacatat
    15541 aaaggctttt tccaattcaa acggatttca tgaaaaatct ggtctgtgtc cgtaaattct
    15601 tttccctgca aaaacaattt catgggttcc ccttcaacag atagatcact gatctgcagt
    15661 cctcgatccg caaaattcgc cccttcaaca aagtggacat tgcttaaaat cgcttcatga
    15721 ttggcaagat aattcaagat ccaaaaaact tctcttcggc taaaagatac atgattgacc
    15781 atccacgtta aaaaattctt cttttcccgt acatcaatta gcatttttct cacctctctg
    15841 ttaggtcatc taataatgac agtgcctcca tatctcctgg ttcaagtgtt aaataataag
    15901 tcaataattc ttgcgctctt ttcagctgcc cttcttctct taagaaaaag gcatactctt
    15961 tcaagaaatc tggttcatgt gatagttcat gataagcctg ttcatagtgt acagctgcaa
    16021 cggtaaagtc ttctaattca ttataagcat gggctaggtt ccattctgca taaggattag
    16081 cagtttcttc catctgattg atcacttgta tcacatcttc atatctttca tcatccaagt
    16141 aaagattact taaagtaagt aatgtttcat cttgtttgtc tcctacttct aaagctttca
    16201 gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa agcattttcc gaggccaggt
    16261 gataaagttc gacttggtaa ggattttctt tgattccttc ttctaacact gtttgtgctt
    16321 cttcaatcat ttcttcttct tgaagagctt gtcctagata tagatataac gattgataat
    16381 gcggattaac ttctcgaagt tcttccagat agttgattgc ctgctgattt tctttcaatt
    16441 gaagattcgt aaaagctagc tggaacagtc gatcatctgt cctttcttct ttcagtgctt
    16501 cttctaaagg tacgatagct gcttcgaatt ctccttgcat acttaaggat tgtcctaaac
    16561 gttcctgcat cgagattcct gaaatttcat ttatcccgct tgccaacaat gtttcataaa
    16621 ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa ttcagctagc gcaaactgga
    16681 tcaatggttc atcaggaagc aacccagctg cttctttcaa ttttgcttca ctcacttcag
    16741 ggattccgat aacttggtac aaatcagcta tcgccaataa gctttgcgga tagtaatcac
    16801 tttgcttatc cactttttcc aggtattcaa aagctaagtc gatctcatta tcttcgattg
    16861 caatttctgc taacgggatg ttcagtccgt cattttctgg gtattggttg atcagttgtt
    16921 caaaaatctg ttttgcttcg atcaaaaagc caatagccag caattcttcg cctagttcag
    16981 ctaatatatc tgctgggtct tcttttaatg ctttttctaa aaagatttgt gcttgtgaca
    17041 agtcttcatc tgataaagct tgcaacattt tttcgctatt tgtattcatt tcaattccct
    17101 cttatctcac tgacgatttg ttgaaatacc gtcattgttc tcaataattc tttttcgttt
    17161 ggatgttggt atccgtctat tatacctatt ttttctaaaa caagtatttt tttgcttttt
    17221 tctgccaaaa aaagaacttt ttccaaatac tccatctttg aaatctgttc cagccagtgg
    17281 attggatagc caagcaagtc cagccacaca aaaaagtttt ttagttggaa atcaaaagga
    17341 tatgcttgga gattccacac gaaatgaaac atcattccta aaaatttttt catactagca
    17401 gaaagtagat ggccattttc ggtcagataa aaagcttgtt caaacagttt accataattt
    17461 gacaggtttt ctgcctctaa ttgataaaaa tagatcattt cttcgatcat cccggcaaac
    17521 ggccgagtcg ttaattgctt tttatttgga tagtttttat acagattttg caaaaaacga
    17581 tgatcacaaa tcattccgca acagataaaa gtcaaaaagt caatctgctt tccgtccacc
    17641 tgccgctcgc tgattgtttg atcataaacg atgcgttctg gcagattgat ggtttgcagc
    17701 actacacgat ttggtagctg caaaatcgtc cgctgcggtg ttaatgctcg gcttagagaa
    17761 cggatcgata cagggatcat ccatagtgat gaatcaaggg ttgtatgttt ttgaaaaaaa
    17821 cctgccagat ccatgatccc ttcatttcca aatccgatga taaacatgga ttgattttcg
    17881 ggatagcgtt tgacgaaatc aagcaggttc tttaattctg ttaaatgatt gcattgagtg
    17941 ttggcacaaa tataccagtc attgtttgct tgatttgcaa ataacgggtt gatcttatct
    18001 gcaaacaaat cataatagcg ctgattcgtt aaaaataaga ttggctgatc aatggaaagt
    18061 tctcttagct gactgactag cgtttctccg taaaacactt tcgtcaacgc atcttctact
    18121 tgataatcaa gttccatacg catccctcct cttcttattt ttattttacc attagtaagc
    18181 aaattctaga aaagataaga gcaaagctca cctcataact tgcaaataaa taaaaattaa
    18241 ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt ctcttattta
    18301 ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc acaagcgctt
    18361 tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa tgcaggtact
    18421 ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt gcgagctgcg
    18481 cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc gttagctaaa
    18541 gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt tttagttaaa
    18601 tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga tagattcacc
    18661 tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag tggtccagaa
    18721 gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca tatgaaccgc
    18781 ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa acgtaacaaa
    18841 cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc tattttctcc
    18901 tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg atttgatttt
    18961 ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa acgacaaatg
    19021 taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt cctttatctg
    19081 ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca gccgaagaga
    19141 tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt tccaaccgtt
    19201 gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg aattcatcac
    19261 gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga tcccatttat
    19321 tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact cgcttgtctt
    19381 gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa cggtcaatgg
    19441 cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt ccgcgtttac
    19501 gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg aagtgggtgt
    19561 cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct tctcctaaaa
    19621 tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta aacttaatcg
    19681 tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca tctaaaatat
    19741 caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc cctaaggaat
    19801 aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact gctaaaatca
    19861 ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct gtcacgcctt
    19921 cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt tctgcttgat
    19981 gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg tcgatcacgc
    20041 tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt actccaggag
    20101 tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta gacttaccga
    20161 cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct cctcacaagt
    20221 ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga ttgtcaatta
    20281 aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa aaactgagac
    20341 aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc agaatcatcg
    20401 gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc catagtaaat
    20461 ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc ttctgaagct
    20521 ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg atggatctct
    20581 aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt cgcaatatgt
    20641 ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac ttcaacaaag
    20701 gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac tggagctttt
    20761 gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga aacacgttct
    20821 tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc caatacatca
    20881 cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc gtcaattccg
    20941 cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact gagaacatct
    21001 ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt ttccactacc
    21061 gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat tttaaagctc
    21121 aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac cattgatgca
    21181 gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac atttgtaaca
    21241 ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca aactttttta
    21301 gcatctaaac ggcgttttga aagtagatag ctgccgtttt ctttatcttt gccgatcgta
    21361 ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat atcttctacc
    21421 ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc ttcgattccg
    21481 acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc gccaactttt
    21541 acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg attttgatca
    21601 aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg gtactgtttt
    21661 tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct tgaaaaaaca
    21721 attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc aaaacaacag
    21781 cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata cgaacagcat
    21841 cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct ctttcctgta
    21901 tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc tctttgaaac
    21961 gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct gcattcggta
    22021 atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc cctatttttc
    22081 gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca gaaacagctt
    22141 ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg aatacatgct
    22201 gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa actagccctt
    22261 cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg cgatacatgg
    22321 caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca acggtacttt
    22381 tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata taactccttt
    22441 ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat tcttgaccag
    22501 ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga tcagtggtga
    22561 tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc gttgttgaac
    22621 cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa tcccctgctt
    22681 gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg ctttctgacg
    22741 ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat tctactgttg
    22801 aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact gatgtcgaag
    22861 tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc cagaaataag
    22921 cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa agaaataatg
    22981 tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca tctgtatcgt
    23041 agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga tgtctatctt
    23101 ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata ggaaaaagtg
    23161 tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta aaatcgttcc
    23221 ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt ctgcttttgc
    23281 tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa aataagctaa
    23341 gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct gcaggcggaa
    23401 ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt tttcagactg
    23461 ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct ctgtggcata
    23521 ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa agaaataatg
    23581 gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct gatctctgcc
    23641 tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat aatggaccac
    23701 ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa tgagaaactg
    23761 cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat caagaccacc
    23821 gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt cgacttcttt
    23881 ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt gttcaagttc
    23941 ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat tgactgattg
    24001 tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg ctactaaatc
    24061 tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc ggatttttcc
    24121 taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat gagcctcatc
    24181 tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag gattagtcaa
    24241 catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga cgtatcttct
    24301 ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc gtttttgtaa
    24361 ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga ccagaccttc
    24421 cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag gcaaaatagc
    24481 gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg gacgaaatga
    24541 atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct tcctttctca
    24601 atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct acattccatt
    24661 cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg attgtttctg
    24721 cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca ataagatgat
    24781 ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt gtctgaccat
    24841 tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt aataatgaat
    24901 cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac aaaaggtgga
    24961 tacatctgct ttgttgcaga taaaccgctg actcatcatt gttttttgcc agctgataag
    25021 gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct gctgcttctt
    25081 ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc aaaatttcac
    25141 gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa ggacttgtct
    25201 ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga acagcaaatt
    25261 taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc aaaccagtat
    25321 gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt gcttcatttt
    25381 catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa aacttatcct
    25441 gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat ccataaataa
    25501 gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc cgcagcttgt
    25561 gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta agttaggatg
    25621 gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat gcggtctttg
    25681 tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt tatttgccca
    25741 agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg taaaaaaagc
    25801 gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa tataaacgaa
    25861 actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc tgtttctctt
    25921 gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt taattgacta
    25981 gttttttcac ggaccctata ggacccattt atattatttc aactttttca gccagttttc
    26041 caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct ctccatctcc
    26101 tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg atacatactc
    26161 tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca atactttatc
    26221 ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc cttttttgtc
    26281 gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat tcttattgac
    26341 ataacgactt gccattacgc cacctaatga atggccaccg atcacataat cggaatttgg
    26401 aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat caagtggcat
    26461 tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa tactataact
    26521 ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc catgattctt
    26581 tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta ccaacacttg
    26641 cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg ctccaccgag
    26701 taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca ctttctccca
    26761 ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct taaaaaaata
    26821 agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc atttgaatat
    26881 ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc ttctgcataa
    26941 atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg gtgcataaag
    27001 tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt acgagcaaaa
    27061 gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg atatgtcccc
    27121 aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag gaagtcaaat
    27181 tcttcataag tcaacccata tttttctgct gttttagtta atttagctac taaatcatct
    27241 ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc tggtgtgatt
    27301 tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt tttgcccacg
    27361 acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat tgggccaaaa
    27421 tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc ttgtgctgca
    27481 tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc ttcgaaatcg
    27541 aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga gtaaggagct
    27601 gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt cgttcctggt
    27661 cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa ttcagcatct
    27721 ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa acaacgccat
    27781 aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc cacgccagca
    27841 tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc atcttgggat
    27901 ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt ttcccaatta
    27961 ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa ttcatctcca
    28021 gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct aaatcctagc
    28081 ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc gaagggatgt
    28141 ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc gctcaatgat
    28201 tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt atcctcctaa
    28261 tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc aaataatgac
    28321 gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa agaaaaaaca
    28381 ctcactgaat ggagctattg acttatccac gtgcttatcg atagccgttt atcggtttct
    28441 tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt gctgctacac
    28501 ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta aatttccacc
    28561 acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca gatgttggtg
    28621 taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa tccttaccga
    28681 cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct aagccggaag
    28741 ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg atgaatacac
    28801 ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct gtgattaatc
    28861 cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct acaccgatca
    28921 aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg ccataagtga
    28981 ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag taccagtcac
    29041 ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt gttaaccagt
    29101 ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga atcaaactga
    29161 tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt tttttcgtga
    29221 ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct ttacgttggt
    29281 tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga tacacatata
    29341 caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata ccttcaccca
    29401 tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc gttgaagcta
    29461 gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta tcaaagccta
    29521 cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct gccattccgt
    29581 aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt ttttcttttc
    29641 ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt ttgttgataa
    29701 tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca gcaggtgttt
    29761 ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc atgaaaatat
    29821 cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg atgttgcctg
    29881 catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg attactgcaa
    29941 taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt tttgtttgtt
    30001 ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta atgttatttt
    30061 atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt ttgttcaaaa
    30121 agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc ttctttttca
    30181 ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg agaagaattt
    30241 cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact caaaacaaac
    30301 aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa agaccaagaa
    30361 atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga actgatgttg
    30421 ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag cactcacgat
    30481 gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg ctaaagacat
    30541 gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg taataacgaa
    30601 ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga cacctaaaaa
    30661 tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg ggaatgtaaa
    30721 gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag aaaaacctgt
    30781 agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg ctggaccgaa
    30841 taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa aactaaaagc
    30901 cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa acatcgctac
    30961 tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta ggactcctct
    31021 tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca gggaaaatag
    31081 agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat ccagactata
    31141 ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt cgtggactat
    31201 aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa tttttgttta
    31261 catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata aaaagtaagt
    31321 gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc acggtaatcc
    31381 ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag tttttgcaca
    31441 ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc gtggatcgtc
    31501 aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa tttagctgga
    31561 gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat cgattttgta
    31621 gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg atgtgtcagg
    31681 cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc gtaatccagt
    31741 cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac aacttttctt
    31801 cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa atagtaaccg
    31861 tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt cccaactttt
    31921 atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag gatgaattct
    31981 tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa tctctccatt
    32041 acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg tcttcttcaa
    32101 gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa ccaaaataat
    32161 ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct actctacctt
    32221 tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc tgaactcctc
    32281 gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt gctaaggttt
    32341 cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta agtaagggag
    32401 ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata tgcaaagctg
    32461 agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg acttctgctt
    32521 cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg ccaacaataa
    32581 aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac tgctggataa
    32641 agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt tttcatcaat
    32701 tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc aaaaaaagaa
    32761 tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac cattcgttct
    32821 tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa tttcttacgt
    32881 tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc tcgcttcaac
    32941 ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta ataaagactt
    33001 cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg gtattctaaa
    33061 agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc gtctgtgacg
    33121 aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc agccatcact
    33181 aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata attcatatat
    33241 tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg ctgaatcaga
    33301 tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat ctccgtcaac
    33361 tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc tctgtgattt
    33421 cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac atttgaaagc
    33481 ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc agctgatcgt
    33541 tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct ggcgtaaaga
    33601 aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac aaagaagttt
    33661 tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg ttttctttcc
    33721 ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa tgctgcttat
    33781 aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta gagatatccg
    33841 cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc agatgtgtgg
    33901 caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc tctcgtacta
    33961 actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga ttattgacaa
    34021 atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct aagtagcgtt
    34081 ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct ccttttccaa
    34141 ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca atcaattcac
    34201 tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt atccctaacg
    34261 tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc aatgtacttg
    34321 gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga tcagtgatac
    34381 gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga gtaacagttg
    34441 ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa aagcgatcga
    34501 catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt tccaaatcac
    34561 gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag tagtggagat
    34621 aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat atcagatacc
    34681 gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc tagacactca
    34741 gacggactgg tttctttttt ctttttcata gcattcctga cagattccat ggaacgttag
    34801 ccgatgatct tttactaaaa aatgataacg ttcttctact acttgctcca cttcaagtaa
    34861 aagatcctct tctacttcct cgatcgtgcc gcagttcaag catagtaaat gatgatggaa
    34921 atgtttggcg ccttcttttc tcaaatcata acgggctaat ccatcattaa aacttacttt
    34981 atcgacaatt ttcaattctg tcaagatttc aagggttcga taaacagtag caagtccaat
    35041 ttccggactc ttttttttca ctaaaaagaa gatttcttct gcagataaat gctctttctc
    35101 attttccaaa agcacaagca gagtagcttc tctttgcggt gtcagcttaa accccgcttc
    35161 atgtagctgc tgttttgttt ttttcatcgc cgaaatagca ttcatgacca tttactcaac
    35221 cccaacaatt tataataatt acaaataaaa aaagatataa cactatgtta attaaaatta
    35281 ttataagcta gttaagaaaa acatgcaagg gattctcaat tagatttttt gactagtttt
    35341 gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa tgcgcgctta
    35401 aatgctcctt tgctgatacc aaaagctttc atgatttctt ctggatccga tttatctgta
    35461 aagttgattt gctgatctgc agaacgttct aaaatggtta aaatcatttg tgcatcatct
    35521 gatatagctt catatgctct tggcttcaat gaaagattca gtacaccgtc tggtcggaca
    35581 ccaatcactc gaccttttat ctgctcgcct agacgaggtt cattatatcg ttcagaagga
    35641 tggataaacc caagatagaa atcttccgtt aaaacgtacg tacctgttaa tttcaagcgg
    35701 tatgctgttc ctatcacatc tttgtttttc atttcttctg ttccatattt gcttaaagat
    35761 ttgaatattt tttcatctgc taaactgccc caaatacggt cttttgcatc aactctaagg
    35821 gaaatcatca agcgatcccc ttttttaggc catagctctg tcattgtcgg caattcatca
    35881 agagatacga caaagtcttt atctggaaga ccgatatcga caaacgcacc taaatctttt
    35941 cgtgtagcag taacgattcc aaaagcgtaa tgaccgatac ggcttttagg aatttgtgta
    36001 gtgatccgat tttcatgttt ttgattttgg taaccgaatc cttcaactgc ttcaccgatt
    36061 ttgtgttcac cttctgattt gcttaagcca aaggtttgtc cgtttttttg gacaaagtag
    36121 tttttttcat tttcatcaat aataatacct gtaaatatag ttgctaataa atcgttcatt
    36181 tttttctccc tctatagtga aaggttttct taatacattt ctttgctatc ataacataat
    36241 gttatctact tgaatagctg agccttggtt tggaaaggtg aaaaaaggag tatgacaaca
    36301 gttgtgtcat actccccttt ttcgaataaa cggtgttaaa cggcaggctc acagcctctt
    36361 tcatttgcct aaaattaatt gctcccattt cctccgaacc atccagtttt acagttgatg
    36421 gttacgatgt aaaatggcaa agcactttct ccaatgcctt tcatcaatgc agattttgtt
    36481 gaagtggcat atagattata atagccttcc ccattttcga ttctggcttt ttctaatctg
    36541 taacctcctt caacgatata tccacgttgg atacatgtag caatcacttt gctttggaca
    36601 cctggtgccc acgtccatgc tggattagat gcatcggcaa tatcacttgc tctggcagat
    36661 ttgattggct gattcgctga gtttgaagag gaaggggtcg aggatgccgc ttcttgctgt
    36721 gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc ttgagcggct
    36781 tgttttgctt gttcttcagc tgcttttttc gcttcttctg cttttttctt ttcctcttct
    36841 tcttttttct ctttatcagt gagcatctta tctacttgtt tcaattgttc attcaactga
    36901 gcagatagat ctgtattttt cactttttct actgcttctt ttgccgtctt gtattggtcg
    36961 cgtgttgctg aatccactac ttttccgtct ttaaagacta tagctgtttt ttctttagct
    37021 gtttgcaatt cagcatattg atttttagct tctgtttgtg cttgttgaat caattttcca
    37081 aaatctgtgt tatccgtttc gattgcttgg atctctttat ctgctttcaa cttgatgtca
    37141 tctgctagat gatcatcgac gatggcaggt gctatgaacc actcattcac tttttggata
    37201 tctcggatct tagattggat atcttggtaa gttgtttcta actctgaata tcctttttca
    37261 ttcttaagac tggataaatc attttttagt tttgataaat cttgatgaat catatctgca
    37321 cgaataaatt gatggtgatc atctgtataa aaggcagcaa gtttctcatg aagttcttcc
    37381 atttgttgct gtctttttgc ttctgctgct acttcggctt ctactttttg atgatggtca
    37441 taataagccc agcttcctcc accgagtaga agtactgctg ctaaaacgag ataaggtact
    37501 ttttttgatt ttttcttttg cgctttatct ggatctgttt tttctaagtt tgattcttga
    37561 tgtgtttttt cagtatctgg cacgattgtt attgatttcg cttctgcttc aaacagctta
    37621 ggctggttct cttctttttc tatttttgct tcaagatcag taccctcttt gcttacagat
    37681 tgattttcag catcggattc actatttatt tcatcgggct ctgttgactt tttgtctgtg
    37741 ttttcgagta tttctttttt ctctgcttcc ggcagtttat tcgttttttc cttttctaat
    37801 tgtttttcag taaatacttc agttttttct gtgatatccg tttttacgtc ttttattttt
    37861 actgtttctt tacctgtggc gttttctcta tcagagtctt tttcagtttc ttgttccttt
    37921 tctggctcta ctttgttttc ctgtgcaatt ttttcactga tcattttgag cagctctttc
    37981 gcgtgctctt cttttgtatc ttcacgatgt gctttgatat aagcggatag tatcgaattt
    38041 tcttcctcat tatcgacgat ttctttcagt tcagctactc ttcttttttg ttttgattca
    38101 agcgtttctt cttgattctt tcctgattct tccgaagtct catgaacagt tgctttcttc
    38161 tgttcatttt tctctatatt agttacttga tcgtgagcag gtggttcttc gtcttctggt
    38221 tctgcttttt cgaataattc cattacagat tctagaggta aatctttcaa ttccgaccat
    38281 tcgattggat cgtttaattc tgtatcagga aagactgcat cttcagttga tttatcctgc
    38341 atagctgcat ctgtatttgt ttcttcaatt gattctttta tttgctgatc ttctgcagtt
    38401 atttgatgac cgcagtctgg acagatagta ttctgctcca tttttttcga tccgcaattt
    38461 ggacattttt tcatcactta gcgatcggct cctttactag gtttacgcta ccatttaatc
    38521 tatcatattc tatacgtcac gccaatagct tatttttagg atttacttaa aaataatgaa
    38581 tgaatttcct tcctatagaa aaaaaagctt gagactgcgt agtctcaagc tttttttctt
    38641 ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca cgcttattct
    38701 aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa tgctttagcc
    38761 tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc ttccaatgtt
    38821 ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc ccgatcagtc
    38881 aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc gtgttctgct
    38941 ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc ttcttcaccg
    39001 attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg gactttttct
    39061 gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc aaggtcttgc
    39121 agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg aactggaata
    39181 cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat ccaccatgta
    39241 gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc cttcatcagt
    39301 cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac ataacgttta
    39361 gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc ttcttggtcg
    39421 ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag ggagacacgg
    39481 ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt tggcgcagaa
    39541 agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct gtgaattgat
    39601 ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg gatcaatttg
    39661 tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa atcatcatat
    39721 acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc ttcatacgtt
    39781 tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat ttggcttgtt
    39841 ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc tgtttgtttc
    39901 cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca gctaagccag
    39961 atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct tctgccaccg
    40021 gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa aagtctaaaa
    40081 actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa aaataaactt
    40141 ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta cggacagaaa
    40201 cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc gtcaaaggtc
    40261 tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc tgaggttctt
    40321 gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc gtctctctgc
    40381 cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt ggtgaaacaa
    40441 ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt tcattatcta
    40501 aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg cgcccatgag
    40561 ccactaattc ttggaaagat gcttctccat acttacggag atattcatca ggatcaagct
    40621 tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc aattcaatcc
    40681 ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc aattctttcg
    40741 tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata gaagctaatc
    40801 cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg aacaacaaaa
    40861 tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa acttgtcttt
    40921 tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt tcctgccctt
    40981 tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa ttacgaagtg
    41041 gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc gtgacaaaca
    41101 atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact tgctgaagaa
    41161 aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct aaagacaacc
    41221 cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat agcatgtgat
    41281 gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca gattcaggat
    41341 tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt tcgaagtctg
    41401 ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga aaagtaaaca
    41461 cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct gcaacagaaa
    41521 aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta ccagactttt
    41581 tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt actgtttcga
    41641 taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg atatcttttg
    41701 ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa ctgaaatcag
    41761 tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat tgctttttca
    41821 agtataacac atttctatct cattaagcat caatttgcta ctcttttcag taactgattt
    41881 tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat gaatacaaag
    41941 aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg gaacagcatt
    42001 gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa tgggttgaag
    42061 ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta atacatcaaa
    42121 gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac cgcaatgata
    42181 ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg caaataacgg
    42241 taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata cccttcaatc
    42301 gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc tgtaacggca
    42361 catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc tgagactttc
    42421 gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt agtaaaatca
    42481 gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc tgccatagca
    42541 atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc tgtcattgca
    42601 accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg cggactcact
    42661 cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc agtaatttga
    42721 gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat attggagaca
    42781 gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg aagataagct
    42841 agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc aaccggctca
    42901 acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa cataagtacg
    42961 cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc ttccaccaaa
    43021 cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc gccccatttg
    43081 cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag ctcaaaataa
    43141 tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata cgtccccata
    43201 atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg gatactcatt
    43261 ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg ggccagcgtt
    43321 tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt tacccctgtc
    43381 gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa caaagctgct
    43441 gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc ttccaaaaat
    43501 agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt gcgaatcgaa
    43561 tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa gcggacagca
    43621 taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt caaaaagctt
    43681 ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc tgtcaaaaga
    43741 gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat ctgttctcct
    43801 gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga ttgttcttta
    43861 ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg tacaagattt
    43921 cttgcatgta tttcttgata aattccaata acgatattga cgacaataat tgctaaatac
    43981 gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa gttcagaaaa
    44041 ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga tgcatcttcc
    44101 tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg tgtaagtcca
    44161 ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg tttaagtcta
    44221 gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa cacacgaatg
    44281 attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt ttttattatc
    44341 aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta gtgctgtacc
    44401 ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg cgtaatcggt
    44461 aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga atagcttgct
    44521 atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac aaagaatccc
    44581 accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt cagccatcgc
    44641 tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga cgatcatacc
    44701 caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga cttgcccgtc
    44761 aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa caaccatgat
    44821 gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg caggaacagt
    44881 tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac ctaataaaaa
    44941 actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa caggcagaaa
    45001 aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa tcagccgctc
    45061 atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga taaatcctga
    45121 accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt accccacttc
    45181 ttgattttcc attttttgcc ttttttccac tatttgcgta ctccttcttt catttaattt
    45241 tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag gtctctatta
    45301 ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta tgatagagat
    45361 agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc ccttcctcat
    45421 ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct actgtgtttt
    45481 ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat gcttggacag
    45541 ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc aaatatttga
    45601 caccttgatc aatactgtca gctggattat caatgatctc agcttctcca tgcagacttt
    45661 ccgagctctg catgagatct gtcccttttc cttttgattc ggtcaatatt atcgccttga
    45721 tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt tctatttgat
    45781 agctttccac tcgttttacc gaacgataag ttcttcctca taaaaacaac accgcaccaa
    45841 taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt tttcgcttca
    45901 tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta tcttcttggc
    45961 tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct gtattttttg
    46021 acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg actgatataa
    46081 tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat gcaaaaatgc
    46141 cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc tttttactgc
    46201 atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc gaacgctact
    46261 ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa gcatctatgt
    46321 tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa aaaatcgatc
    46381 aggcagattt tcttggattt agtgatggag caaatctagc actggtattt gccagcagct
    46441 tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt aaaggcgttc
    46501 gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta ctctctttat
    46561 ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat gacatcgggc
    46621 ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg ggaaaaaaag
    46681 atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa gcttcctttg
    46741 tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc ttcaatcgag
    46801 aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat ttatttgtta
    46861 aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat ctttttgatt
    46921 tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct atctatccag
    46981 caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat gctattgatt
    47041 ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg gatgcttgaa
    47101 caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac gatcaacaat
    47161 attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata tggacaaaag
    47221 aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt gatggctgga
    47281 ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt tgctcctttt
    47341 ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt cgtcttttta
    47401 tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac tcaaggacag
    47461 ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct ttctgttggt
    47521 tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat tgctgcttca
    47581 gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt aatgatgatt
    47641 atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat cttgctctat
    47701 cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt caaaaatatc
    47761 ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac agaaattgcg
    47821 cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact ttctgccact
    47881 atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt gaaatttcat
    47941 tttattatcc aatttcctag tatactattg ggatttcttt taatcattat gggcagaggg
    48001 atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt tctagcttta
    48061 ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc tattctgctt
    48121 ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta ttcatgggag
    48181 tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat cgttattggg
    48241 gtatacaatc tgccggattt ccctcatcgg cgtcaccatt ttatttcctt tttcttattt
    48301 ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag tttcatgatc
    48361 gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc ctttaatgaa
    48421 gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca attgatcttc
    48481 ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt tcttctccaa
    48541 ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa gaaagaagat
    48601 tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta tttaccagtt
    48661 ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta cgatttcatt
    48721 aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg gaaaaaaatg
    48781 aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt tgatgtctta
    48841 caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga taattggttg
    48901 ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta tctacaaaga
    48961 gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc aacgatcatg
    49021 cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga ttctaaaaaa
    49081 gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat gaaagaagaa
    49141 catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac ttccagaaag
    49201 agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca tttttattct
    49261 ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc acgttatact
    49321 ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat tattgataat
    49381 gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat acgaagaaag
    49441 tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga gactgaagta
    49501 aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa agagcggtcc
    49561 aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta ccaaaatatt
    49621 cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact gtccgtccgc
    49681 cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc aagtcaacat
    49741 caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat gtgattgttc
    49801 ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag aatggagtat
    49861 gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt gtatgtggag
    49921 taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt tggatccccc
    49981 gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta cggaacattt
    50041 ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc tctacctcat
    50101 caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct cgacctgtaa
    50161 ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt tctggtgttg
    50221 ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca tctggatcgc
    50281 cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct gggaaaccat
    50341 attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct tcatcatcga
    50401 ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg cgtgacaata
    50461 aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact aagattgcac
    50521 catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt cctggtgcat
    50581 caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta ttgatcgtaa
    50641 tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga ggatttgcta
    50701 ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc ccatggtcaa
    50761 cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt tcttttgcca
    50821 taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct caccttttta
    50881 ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt tgtaagattc
    50941 gaactaagct tattatacgt caatattggt caaagtcaac taagagcact cgtctgtctt
    51001 atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa aaacttcaag
    51061 atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt taaaattcag
    51121 ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata aggaaagagg
    51181 tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt ctcggcggaa
    51241 gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa atcacgacga
    51301 atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact tatacctaca
    51361 agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag
    51421 aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt aaaggtgtat
    51481 taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata
    51541 attagtaaat ggtcttaaaa ttttattaga taaaa
//
LOCUS       DAYYCQ010000010        50048 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000010 DAYYCQ010000000
VERSION     DAYYCQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.010"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHAYF_000624"
     CDS             complement(105..629)
                     /locus_tag="ACHAYF_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8578088.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHAYF_000625"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHAYF_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8578089.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHAYF_000626"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHAYF_000626"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8578090.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHAYF_000627"
     CDS             complement(2351..3031)
                     /locus_tag="ACHAYF_000627"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8578091.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHAYF_000628"
     CDS             3157..4026
                     /locus_tag="ACHAYF_000628"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8578092.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHAYF_000629"
     CDS             4052..4567
                     /locus_tag="ACHAYF_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8578093.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHAYF_000630"
     CDS             complement(4614..5177)
                     /locus_tag="ACHAYF_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8578094.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHAYF_000631"
     CDS             complement(5232..5411)
                     /locus_tag="ACHAYF_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578095.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHAYF_000632"
     CDS             complement(5488..5997)
                     /locus_tag="ACHAYF_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578096.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHAYF_000633"
     CDS             complement(6093..6473)
                     /locus_tag="ACHAYF_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8578097.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHAYF_000634"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHAYF_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8578098.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACHAYF_000635"
     CDS             complement(7736..9013)
                     /locus_tag="ACHAYF_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578099.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHAYF_000636"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHAYF_000636"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8578100.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACHAYF_000637"
     CDS             complement(9935..10879)
                     /locus_tag="ACHAYF_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8578101.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="ACHAYF_000638"
     CDS             complement(10881..11567)
                     /locus_tag="ACHAYF_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8578102.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="ACHAYF_000639"
     CDS             12041..12361
                     /locus_tag="ACHAYF_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8578103.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="ACHAYF_000640"
     CDS             12515..13420
                     /locus_tag="ACHAYF_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578104.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="ACHAYF_000641"
     CDS             13417..14190
                     /locus_tag="ACHAYF_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8578105.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="ACHAYF_000642"
     CDS             complement(14371..15795)
                     /locus_tag="ACHAYF_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8578106.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="ACHAYF_000643"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="ACHAYF_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8578107.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="ACHAYF_000644"
     CDS             complement(16517..18499)
                     /locus_tag="ACHAYF_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8578108.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="ACHAYF_000645"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="ACHAYF_000645"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8578109.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="ACHAYF_000646"
     CDS             complement(19412..20473)
                     /locus_tag="ACHAYF_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8578110.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="ACHAYF_000647"
     CDS             complement(20684..21211)
                     /locus_tag="ACHAYF_000647"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8578111.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="ACHAYF_000648"
     CDS             complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="ACHAYF_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8578112.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="ACHAYF_000649"
     CDS             complement(23038..24159)
                     /locus_tag="ACHAYF_000649"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8578113.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="ACHAYF_000650"
     CDS             24285..25226
                     /locus_tag="ACHAYF_000650"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8578114.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="ACHAYF_000651"
     CDS             complement(25376..26272)
                     /locus_tag="ACHAYF_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8578115.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="ACHAYF_000652"
     CDS             26541..27881
                     /locus_tag="ACHAYF_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8578116.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="ACHAYF_000653"
     CDS             28099..28542
                     /locus_tag="ACHAYF_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8578117.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="ACHAYF_000654"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="ACHAYF_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8578118.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="ACHAYF_000655"
     CDS             complement(30867..32090)
                     /locus_tag="ACHAYF_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8578119.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="ACHAYF_000656"
     CDS             complement(32121..32960)
                     /locus_tag="ACHAYF_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8578120.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="ACHAYF_000657"
     CDS             complement(32977..33657)
                     /locus_tag="ACHAYF_000657"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8578121.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="ACHAYF_000658"
     CDS             complement(33659..34732)
                     /locus_tag="ACHAYF_000658"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8578122.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34788..35026)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35069..35302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35379..36050)
                     /locus_tag="ACHAYF_000659"
     CDS             complement(35379..36050)
                     /locus_tag="ACHAYF_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8578123.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38785)
                     /gene="mgtA"
                     /locus_tag="ACHAYF_000660"
     CDS             complement(36137..38785)
                     /gene="mgtA"
                     /locus_tag="ACHAYF_000660"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8578124.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(39127..39301)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="ACHAYF_000661"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="ACHAYF_000661"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8578125.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="ACHAYF_000662"
     CDS             complement(39933..40799)
                     /locus_tag="ACHAYF_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8578126.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="ACHAYF_000663"
     CDS             complement(40959..43052)
                     /locus_tag="ACHAYF_000663"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8578127.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43801..44424)
                     /locus_tag="ACHAYF_000664"
     CDS             complement(43801..44424)
                     /locus_tag="ACHAYF_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578128.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44583..45980)
                     /locus_tag="ACHAYF_000665"
     CDS             complement(44583..45980)
                     /locus_tag="ACHAYF_000665"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8578129.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46012..46983)
                     /locus_tag="ACHAYF_000666"
     CDS             complement(46012..46983)
                     /locus_tag="ACHAYF_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8578130.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47357..47782
                     /locus_tag="ACHAYF_000667"
     CDS             47357..47782
                     /locus_tag="ACHAYF_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578131.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48270..48971)
                     /locus_tag="ACHAYF_000668"
     CDS             complement(48270..48971)
                     /locus_tag="ACHAYF_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8578132.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48987..49427)
                     /locus_tag="ACHAYF_000669"
     CDS             complement(48987..49427)
                     /locus_tag="ACHAYF_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8578133.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49427..50002)
                     /locus_tag="ACHAYF_000670"
     CDS             complement(49427..50002)
                     /locus_tag="ACHAYF_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8578134.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        14421 a        10480 c         8733 g        16414 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtga taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    43681 acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    43741 attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt
    43801 tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg
    43861 attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt
    43921 cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca
    43981 ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac
    44041 ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa
    44101 atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt
    44161 aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta
    44221 attggcagtt attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc
    44281 tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat
    44341 ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag
    44401 aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta
    44461 ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa
    44521 cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt
    44581 tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg
    44641 attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat
    44701 gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca
    44761 ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa
    44821 tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg
    44881 ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca
    44941 tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt
    45001 tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct
    45061 tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct
    45121 ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc
    45181 tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag
    45241 aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag
    45301 cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc atggtaagat
    45361 ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata
    45421 tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt
    45481 tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca
    45541 tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac
    45601 atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca
    45661 tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt
    45721 atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac
    45781 tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca
    45841 aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg
    45901 cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct
    45961 ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat
    46021 tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat
    46081 tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt
    46141 gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat
    46201 atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt
    46261 gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata
    46321 gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg
    46381 gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc
    46441 aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact
    46501 ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc
    46561 gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa
    46621 gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt
    46681 ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct
    46741 ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc
    46801 aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa
    46861 cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa
    46921 gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc
    46981 catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat
    47041 gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt
    47101 agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt
    47161 aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg
    47221 attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg
    47281 tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc
    47341 aaatggggga agtattttga agaaattaat cgtactattt attttgtctg cgggactttt
    47401 gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga
    47461 ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt
    47521 acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg
    47581 gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt
    47641 gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa
    47701 taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga
    47761 tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt
    47821 ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc
    47881 ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa
    47941 gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat
    48001 cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc
    48061 actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag
    48121 taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca
    48181 aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt
    48241 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
    48301 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
    48361 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
    48421 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
    48481 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
    48541 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
    48601 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
    48661 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
    48721 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
    48781 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
    48841 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
    48901 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
    48961 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
    49021 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
    49081 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
    49141 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
    49201 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
    49261 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
    49321 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
    49381 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
    49441 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
    49501 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
    49561 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
    49621 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
    49681 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
    49741 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
    49801 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
    49861 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
    49921 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
    49981 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
    50041 tacacgtg
//
LOCUS       DAYYCQ010000011        48299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000011 DAYYCQ010000000
VERSION     DAYYCQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.011"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHAYF_000671"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHAYF_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8578135.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHAYF_000672"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHAYF_000672"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8578136.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACHAYF_000673"
     CDS             complement(2298..2960)
                     /locus_tag="ACHAYF_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8578137.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACHAYF_000674"
     CDS             3144..4790
                     /locus_tag="ACHAYF_000674"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8578138.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHAYF_000675"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHAYF_000675"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8578139.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7524)
                     /locus_tag="ACHAYF_000676"
                     /pseudo
     CDS             complement(5971..7524)
                     /locus_tag="ACHAYF_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7672..8813
                     /locus_tag="ACHAYF_000677"
                     /pseudo
     CDS             7672..8813
                     /locus_tag="ACHAYF_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8939..10018)
                     /locus_tag="ACHAYF_000678"
     CDS             complement(8939..10018)
                     /locus_tag="ACHAYF_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8578140.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10121..10561)
                     /locus_tag="ACHAYF_000679"
     CDS             complement(10121..10561)
                     /locus_tag="ACHAYF_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8578141.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10618..11028)
                     /locus_tag="ACHAYF_000680"
     CDS             complement(10618..11028)
                     /locus_tag="ACHAYF_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8578142.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11208..11642)
                     /locus_tag="ACHAYF_000681"
     CDS             complement(11208..11642)
                     /locus_tag="ACHAYF_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8578143.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11742..12590)
                     /locus_tag="ACHAYF_000682"
     CDS             complement(11742..12590)
                     /locus_tag="ACHAYF_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8578144.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12979..14745
                     /locus_tag="ACHAYF_000683"
     CDS             12979..14745
                     /locus_tag="ACHAYF_000683"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8578145.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14847..16106
                     /locus_tag="ACHAYF_000684"
     CDS             14847..16106
                     /locus_tag="ACHAYF_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8578146.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16253..17554
                     /locus_tag="ACHAYF_000685"
     CDS             16253..17554
                     /locus_tag="ACHAYF_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578147.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17557..18408
                     /locus_tag="ACHAYF_000686"
     CDS             17557..18408
                     /locus_tag="ACHAYF_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8578148.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18421..19236
                     /locus_tag="ACHAYF_000687"
     CDS             18421..19236
                     /locus_tag="ACHAYF_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8578149.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19387..20553
                     /locus_tag="ACHAYF_000688"
     CDS             19387..20553
                     /locus_tag="ACHAYF_000688"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8578150.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20783..21430
                     /locus_tag="ACHAYF_000689"
     CDS             20783..21430
                     /locus_tag="ACHAYF_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8578151.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21514..22458
                     /locus_tag="ACHAYF_000690"
     CDS             21514..22458
                     /locus_tag="ACHAYF_000690"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8578152.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22593..24044
                     /locus_tag="ACHAYF_000691"
     CDS             22593..24044
                     /locus_tag="ACHAYF_000691"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8578153.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24065..24901
                     /locus_tag="ACHAYF_000692"
     CDS             24065..24901
                     /locus_tag="ACHAYF_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8578154.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24901..25560
                     /locus_tag="ACHAYF_000693"
     CDS             24901..25560
                     /locus_tag="ACHAYF_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8578155.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            25686..26375
                     /locus_tag="ACHAYF_000694"
     CDS             25686..26375
                     /locus_tag="ACHAYF_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578156.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26381..27337
                     /locus_tag="ACHAYF_000695"
     CDS             26381..27337
                     /locus_tag="ACHAYF_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8578157.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27381..27893
                     /locus_tag="ACHAYF_000696"
     CDS             27381..27893
                     /locus_tag="ACHAYF_000696"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8578158.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28149..28697)
                     /locus_tag="ACHAYF_000697"
     CDS             complement(28149..28697)
                     /locus_tag="ACHAYF_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8578159.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28786..29967)
                     /locus_tag="ACHAYF_000698"
     CDS             complement(28786..29967)
                     /locus_tag="ACHAYF_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8578160.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30203..31711)
                     /locus_tag="ACHAYF_000699"
     CDS             complement(30203..31711)
                     /locus_tag="ACHAYF_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8578161.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31870..32061
                     /locus_tag="ACHAYF_000700"
     CDS             31870..32061
                     /locus_tag="ACHAYF_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578162.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32264..33181
                     /locus_tag="ACHAYF_000701"
     CDS             32264..33181
                     /locus_tag="ACHAYF_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8578163.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33174..34247
                     /locus_tag="ACHAYF_000702"
     CDS             33174..34247
                     /locus_tag="ACHAYF_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578164.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34395..34913
                     /locus_tag="ACHAYF_000703"
     CDS             34395..34913
                     /locus_tag="ACHAYF_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578165.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34894..35286
                     /locus_tag="ACHAYF_000704"
     CDS             34894..35286
                     /locus_tag="ACHAYF_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8578166.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35362..36297
                     /locus_tag="ACHAYF_000705"
     CDS             35362..36297
                     /locus_tag="ACHAYF_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8578167.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36430..37209
                     /locus_tag="ACHAYF_000706"
     CDS             36430..37209
                     /locus_tag="ACHAYF_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8578168.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37202..37519
                     /locus_tag="ACHAYF_000707"
     CDS             37202..37519
                     /locus_tag="ACHAYF_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8578169.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37763..39373
                     /locus_tag="ACHAYF_000708"
     CDS             37763..39373
                     /locus_tag="ACHAYF_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8578170.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39530..40378
                     /locus_tag="ACHAYF_000709"
     CDS             39530..40378
                     /locus_tag="ACHAYF_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8578171.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            40609..41088
                     /locus_tag="ACHAYF_000710"
     CDS             40609..41088
                     /locus_tag="ACHAYF_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8578172.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            41121..43349
                     /locus_tag="ACHAYF_000711"
     CDS             41121..43349
                     /locus_tag="ACHAYF_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8578173.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            43478..45121
                     /locus_tag="ACHAYF_000712"
     CDS             43478..45121
                     /locus_tag="ACHAYF_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8578174.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45194..45739)
                     /locus_tag="ACHAYF_000713"
     CDS             complement(45194..45739)
                     /locus_tag="ACHAYF_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8578175.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(45762..46247)
                     /locus_tag="ACHAYF_000714"
     CDS             complement(45762..46247)
                     /locus_tag="ACHAYF_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8578176.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(46237..47049)
                     /locus_tag="ACHAYF_000715"
     CDS             complement(46237..47049)
                     /locus_tag="ACHAYF_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8578177.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(47188..>48299)
                     /locus_tag="ACHAYF_000716"
     CDS             complement(47188..>48299)
                     /locus_tag="ACHAYF_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8578178.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        15897 a         8764 c         9362 g        14276 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt
     7501 tttaatcgct tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt
     7561 gaaaatatta ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa
     7621 tctctactat cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat
     7681 tcaaccatct taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca
     7741 gtccacacag aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat
     7801 cgaattacct ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa
     7861 ggacaaacag cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta
     7921 caatcagctg ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac
     7981 actttgacta tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac
     8041 ttgttaaaac cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat
     8101 ttaacgccgt atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga
     8161 gatcttgttg aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct
     8221 acagaaaatc cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca
     8281 gaaactcaac cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca
     8341 gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
     8401 gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca
     8461 gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac
     8521 aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta
     8581 aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct
     8641 gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc
     8701 taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa
     8761 gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga
     8821 ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat
     8881 accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt
     8941 aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta
     9001 aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc
     9061 taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact
     9121 tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa
     9181 caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga
     9241 taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct
     9301 gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa
     9361 caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa
     9421 tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt
     9481 ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca
     9541 tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa
     9601 aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg
     9661 gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca
     9721 tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga
     9781 tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca
     9841 tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa
     9901 gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac
     9961 gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta
    10021 tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
    10081 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
    10141 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
    10201 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
    10261 caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
    10321 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
    10381 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
    10441 caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga
    10501 tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
    10561 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
    10621 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
    10681 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
    10741 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
    10801 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
    10861 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
    10921 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc
    10981 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc
    11041 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
    11101 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
    11161 acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc
    11221 tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat
    11281 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
    11341 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
    11401 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
    11461 agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca
    11521 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
    11581 tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc
    11641 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta
    11701 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
    11761 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
    11821 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
    11881 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
    11941 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
    12001 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
    12061 gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg
    12121 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
    12181 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
    12241 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
    12301 tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag
    12361 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
    12421 actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct
    12481 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
    12541 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt
    12601 ccttaattca ttaataatac tcttatctta ttataactta cttttcacct taagttaact
    12661 ttaattaata aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac
    12721 ttctacgcca aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt
    12781 aagataaatt caggacacta aatagtacag gaagtagact aaaactttta aaagttttaa
    12841 gtctatctat ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata
    12901 acatttttga aagcgttgtc ttttgcttct ctatcactta tagtgatgac gtcatattaa
    12961 aacacaagga ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat
    13021 gcatttctat atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc
    13081 aaaaaagtat atgtgctatg tggagatcca tataccataa caactgaaaa atggtatcaa
    13141 aaacaacgac caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa
    13201 gtgacgagcg aaacaagtcg cttacagtat gcgttccatg ttgttgggga agataacacg
    13261 gactgttttt acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca
    13321 aatttttatt ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg
    13381 gtaaaagaaa cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca
    13441 aatgaccccg aacacacttt accttgggga agtaaagatc cagaccgaga agactttttc
    13501 gggggagatt tgcaaggtgt gatggatcat ttggattact tgacggacct tggcatcaat
    13561 ggcatctatt tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat
    13621 tattatgagg tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa
    13681 gcacataaaa gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat
    13741 tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc
    13801 cacatccgtt ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa
    13861 aatatcccat atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat
    13921 ccagaagtgc aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt
    13981 gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga
    14041 gaagcagtta cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct
    14101 caagcttggc tgcaagggga tgaattccat gcagtgatga attatgcatt tacagattcg
    14161 attaaagatt attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat
    14221 catcagcaaa tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct
    14281 catgatactg ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg
    14341 atggccttta tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg
    14401 atgactggtg aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa
    14461 gatctagagc tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag
    14521 attatttctg aaggttctac tcagtggcta atagtaaatg atcgtgaaga taagctctat
    14581 ttcacacgga aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct
    14641 tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac
    14701 gggaaagcag agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta
    14761 acaacaagaa agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca
    14821 cgttgaatac aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag
    14881 ggttaatctc tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag
    14941 cagaagggga agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag
    15001 attatataaa cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag
    15061 tagaaaagga catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg
    15121 gacctgacgt tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc
    15181 tggcggaagt aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga
    15241 cttatgatgg aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata
    15301 acaaagattt agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag
    15361 acagtcgttt tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga
    15421 ctgactttta ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg
    15481 atggcacaaa tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt
    15541 atgcaacaga ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag
    15601 gagacttcgc cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat
    15661 ggcaagcgca aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc
    15721 taaataatgg acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt
    15781 atgctaaaaa caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc
    15841 aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag
    15901 caaaggctaa aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag
    15961 cgatgccaaa catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg
    16021 atgcagcatc aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca
    16081 gtgaagccat agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg
    16141 gaacaggtgc ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg
    16201 ccattatctt aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca
    16261 agataaaaca agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat
    16321 ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct
    16381 attcattggt cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg
    16441 aagtatacca atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat
    16501 cattacaatc attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc
    16561 gaagatgcgt caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat
    16621 aggtgatgaa ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac
    16681 gatcattttc ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca
    16741 tattccacca gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt
    16801 tttaagctca tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt
    16861 atgtgcaaca actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa
    16921 atttattaaa ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc
    16981 ttttattact atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac
    17041 tcaagtgatt cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa
    17101 aacggacccg aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc
    17161 ctatatctat gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc
    17221 agcaaaaatc gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt
    17281 cttcctagtg gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc
    17341 catgatctat ctgtttaaca atggcggtcc cggaagcgta ggcggaggag ctggttctac
    17401 tgatattctt atttcctgga tctataaact tacgactgga acttctccgc agtattctat
    17461 ggcttcagca atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt
    17521 taaaaagaca aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct
    17581 cgattccaaa aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc
    17641 attatcatct atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca
    17701 gcttttagtt tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt
    17761 gaaacacttt acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc
    17821 gtacaagtga ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc
    17881 cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg
    17941 actgcctttt atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca
    18001 atgatctata taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac
    18061 actgtaccaa tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt
    18121 tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct
    18181 tttatgggac cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat
    18241 cagactgttg ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct
    18301 ctttttgctg ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta
    18361 caaaagaact ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc
    18421 atgcaaacat ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg
    18481 ggacgcaaac tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg
    18541 atggtcatgc ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa
    18601 tttctatccg ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa
    18661 ttatctgaaa ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg
    18721 gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag
    18781 aaggaacaat gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat
    18841 tatagcccat ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta
    18901 gaaagagaat tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt
    18961 ggtcttgtcc tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg
    19021 atgacaagaa aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg
    19081 ctgaatgtta tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac
    19141 tttatcttga tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt
    19201 gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca
    19261 atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac
    19321 ttcttagttg attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg
    19381 aaaatcatgt tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct
    19441 gtcgaagtaa attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc
    19501 caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcatct
    19561 ttggcactag gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca
    19621 ttgatcaacg atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa
    19681 tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat
    19741 tatgacttag gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt
    19801 gcttatttca atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat
    19861 atcttagaca aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg
    19921 ggaacattga tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta
    19981 agtgaagaac aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa
    20041 tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca
    20101 agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta
    20161 aaagatttat tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc
    20221 ggtgcgacaa atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt
    20281 actgaattag ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta
    20341 cgtcgtgact accaattgac attagaacat tggacaaaga attttcatca agtccaagat
    20401 caagtcacga aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct
    20461 tgtgcagctt catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct
    20521 ggaaacaatg acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact
    20581 atttaaaaag aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta
    20641 ggattgtctc taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct
    20701 ttaattttta aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt
    20761 tataacaaag gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc
    20821 tttgttttta gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc
    20881 cagtagcgaa cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc
    20941 gtctagccag acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc
    21001 agctcgtgcg atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga
    21061 attagatagt gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga
    21121 atacgaagca agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct
    21181 agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt
    21241 gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac
    21301 agattgggat ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg
    21361 caatcaaagt acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca
    21421 agatgattaa gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact
    21481 agacaaaata acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat
    21541 catatctttg ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa
    21601 ttagatgaac tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat
    21661 gaacgaaaag gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct
    21721 atgggacttc caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa
    21781 gtgcaggaaa accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa
    21841 aacttggaaa tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat
    21901 ttatcttgtc ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat
    21961 gaaacactga aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct
    22021 tattttgatt ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc
    22081 tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca
    22141 gtagtcatca aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact
    22201 gctttggcga atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt
    22261 acaggtggga ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg
    22321 ttacagattg gtaccgagct tcataaagaa gggccggaaa ttttttctag aatcataaaa
    22381 gaattaacgc agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa
    22441 ttacgcacca tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata
    22501 aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt
    22561 gggaaaataa cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag
    22621 agcttaaaga aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa
    22681 agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc
    22741 acgagctatt gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa
    22801 aaatgctcgg tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa
    22861 cacaccgagc ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc
    22921 cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct
    22981 atggcccagc aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag
    23041 ctgctgctgc agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt
    23101 ctattagaat acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga
    23161 tgccagttgg tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga
    23221 cagtttctat gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg
    23281 cgccatatca agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga
    23341 tagctttttg tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg
    23401 taaaaaaagc tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc
    23461 cactagatgg tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag
    23521 caatgatccg ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac
    23581 ccatttcttg gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt
    23641 ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc
    23701 tattcctttc accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg
    23761 atgcgataag agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg
    23821 ttgtttcttc catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc
    23881 tgactggaca accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag
    23941 gaatccaatt tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat
    24001 ttgaagcaaa tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag
    24061 aagaatgaag aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc
    24121 ttgcggtaca gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa
    24181 agaattagta gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa
    24241 tgaattagtt ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa
    24301 aatctccttt aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc
    24361 agattttgct gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc
    24421 agagactttc aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat
    24481 ccactcactt gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat
    24541 gcgtttagcg gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca
    24601 gtatttaagc gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca
    24661 gaaaatggcc gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt
    24721 caatgaaaac gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat
    24781 cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata
    24841 ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg
    24901 atgtactttc ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa
    24961 ggcttggtat acacattagg gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg
    25021 ttgcttttga cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt
    25081 tatttatcgt tctttagagg cattcccgca ctagtcctgc tgtttctgtt atattttggg
    25141 ttaccttatc aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt
    25201 gcatttattg gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac
    25261 gctggtttag cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa
    25321 gcttttcgaa tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact
    25381 tcgttagcag caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt
    25441 cggacattcg attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt
    25501 atcgggatcg aaaaagtgca acagtattta gaaaaacgtt ctatgcatca gtcaatctga
    25561 aaaatagaaa tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc
    25621 tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag
    25681 aaaacatgaa gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg
    25741 agcaaggcgg cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata
    25801 cgcttagctg cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag
    25861 tgacaaaagc agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt
    25921 ttgttttacc aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg
    25981 ttttagtgta tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga
    26041 ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct
    26101 tcatggcttc tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat
    26161 attttccaac agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat
    26221 tttctaacga acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg
    26281 agttgcaaga agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc
    26341 agatccgtcg gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag
    26401 aggaaaacaa tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa
    26461 taaaatccaa ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga
    26521 attatttcaa aaaaagcacc atcgaatcgc tgtgatccaa aatccccaag taggacaatt
    26581 caaattattc gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc
    26641 tgcacagatc ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct
    26701 tgttttcgat ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt
    26761 tgattcgaaa tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa
    26821 aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac
    26881 agatgacttt tttagtgatg actatctttt tttccacgca gacggatata ttggattttc
    26941 tgatttcacg atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc
    27001 tatccatcta acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga
    27061 cagtaacgac tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa
    27121 aaaatggatc atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt
    27181 ggctctttac cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat
    27241 gcatcatttg gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta
    27301 ttcaaaagtt caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg
    27361 aatagaaaag aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag
    27421 tggtagaaat cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc
    27481 aaaacgggca gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct
    27541 atgtattaat agtggaagat caaattgctg gaacagctgc attagtctca agcgtggatc
    27601 ccgtttacac aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc
    27661 atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac
    27721 atttgttgac gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag
    27781 ctaatgaacc gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg
    27841 aatttccttt tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa
    27901 atcatctgag caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca
    27961 agaggttgta acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc
    28021 tcaaattttt gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc
    28081 atcgagaggt cgtgacatag cttttgtcac gacctctcgg cttatcctac gagtattatt
    28141 cgatacattt atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca
    28201 tcgtttgaca cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct
    28261 tctacataaa ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg
    28321 cgcgattgca atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa
    28381 tactcatata aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc
    28441 agtttctttt taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac
    28501 tctgcaaact catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa
    28561 tcctcttgat ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt
    28621 tccggctgaa aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat
    28681 acatagcaat aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg
    28741 aaaatatttc caaagctttt aaaagtaact ttgctttttt gtatattata ccagcaagcc
    28801 agtcacaata gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg
    28861 ttttgcgaca agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc
    28921 gatagaagag aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc
    28981 agtaaaatgg aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag
    29041 tttagtcgcc atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa
    29101 ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg
    29161 gaagatagca ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt
    29221 gatcgccaca tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc
    29281 tttgttttct tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat
    29341 tgaagccaga atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga
    29401 atcgatcagt gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac
    29461 actcatggtg aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa
    29521 aacttccgat tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat
    29581 cccgttgatt ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat
    29641 atagcgtaga ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa
    29701 gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc
    29761 aaaagcaaga agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt
    29821 tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt
    29881 caatttgatt gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa
    29941 accaataata cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt
    30001 tacctgtttt gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg
    30061 aaacgcattc attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta
    30121 acgtcttagt aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc
    30181 aacatttata agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg
    30241 cgcaggtaat atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca
    30301 cagtagagtg ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga
    30361 ttggattcgt tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat
    30421 ccaaagagga atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg
    30481 acaataaagg aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg
    30541 ccgctgacgc atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt
    30601 cgattgcaat cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat
    30661 agtcatgggc agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa
    30721 tcagtttgat tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg
    30781 tttggaaata acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca
    30841 cgatcccaaa tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt
    30901 taccagtaat tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat
    30961 agttcacagc tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt
    31021 ttagatcgac tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc
    31081 atactgggaa agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat
    31141 acagttggtt caaggaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa
    31201 cgattggttt agttgtttca atatactcat tttttccaaa accatcttct gagacgattt
    31261 tttctacgtt gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc
    31321 tttctagttt attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga
    31381 cactgttgat taataagcca taaccacgta attcatcgat taaccgcaag atatccatat
    31441 cataagtaat cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc
    31501 aaatcagtat ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt
    31561 caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac tagcttccca ccaaattcta
    31621 aatataattt gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat
    31681 atttttctga atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt
    31741 ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa
    31801 aatggtttaa accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga
    31861 aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg
    31921 atttttaccg gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact
    31981 tatctaaacg aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca
    32041 agagatctgc ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat
    32101 cagatggaaa gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt
    32161 tatggagcat tgcccgctct tttttataac aattttttac agaaaatggt actattgaag
    32221 tattaataag tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac
    32281 aaatgcaaat tatgagccag acgaactaga ggagacgaat gcggatcagg atacaggaca
    32341 agaatcgtct tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt
    32401 agaagactta caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg
    32461 tttatacaat ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat
    32521 cgatcaattg ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga
    32581 acaagtagaa aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga
    32641 tcggcccacg gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga
    32701 tgaagagtgg gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga
    32761 gctagacgca aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa
    32821 agaaatcaat cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa
    32881 aatcgaggaa gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt
    32941 aggctggctt ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa
    33001 gcaagaagca agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat
    33061 cagacgctat ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa
    33121 ttatcttggc aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg
    33181 aacacgatga agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga
    33241 acacttcatt ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc
    33301 tagaggaaac aaaaatacac gaaaaagaaa aagtaactcc gacgaatacg aatactgcag
    33361 aaaacaccca tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag
    33421 atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta
    33481 tgagaaaaga tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta
    33541 tattgaatga agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt
    33601 atgaattact gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca
    33661 catggaaaac aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct
    33721 tttcctttgt attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa
    33781 acgtatcacc agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc
    33841 gacactggtc agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat
    33901 tgaatcaatt attagcccat gcaagaacac ttggttttac cgttaatcag acattactag
    33961 acaatacgaa gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat
    34021 tggatgatat ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg
    34081 aaactactta tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa
    34141 aagatcaaac agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct
    34201 ttatcaatta cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg
    34261 attaccagat gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata
    34321 attgtcacaa gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag
    34381 ggatttcatt aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt
    34441 gtgtagcagg taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt
    34501 ttttatttat agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta
    34561 ttgcgatcag cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct
    34621 atactgttgt tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc
    34681 tatggttaaa gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg
    34741 tttttgatac gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt
    34801 cgtttgattt gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg
    34861 tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt
    34921 gcaatgagtg tcagtatact cattttgaca agttgcagta caacctatac acaatcggct
    34981 tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat
    35041 ggtcaaaact tttcggaaaa agaagtgttc ttcaaaaaag gcgatgattt actgagtatt
    35101 ttgaaacgaa attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac
    35161 tcacaggatg agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact
    35221 actggtgcca aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa
    35281 ctataagttt cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt
    35341 tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt
    35401 gctgctctag gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt
    35461 ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt
    35521 ttgctgttat tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt
    35581 gaaggacgag caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt
    35641 ggggcacttg gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt
    35701 aatccgatca ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta
    35761 cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt
    35821 ttgcgcgaaa gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta
    35881 atcggtttgg tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca
    35941 tttgatgcga tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta
    36001 gcaaaagaat tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa
    36061 atcaagttag cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat
    36121 ttaagtccaa cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat
    36181 gaagcttact cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag
    36241 aagatgtata tatacattga aacagataaa tatgacccaa attattctcg taaataaaac
    36301 gtttattcat gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg
    36361 ctacaattta gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga
    36421 gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg
    36481 ccactttgta taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt
    36541 ggatttgatc ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag
    36601 tttttagttg catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt
    36661 ttctttttag aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa
    36721 gaaaagcgga cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg
    36781 aactacttaa agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat
    36841 tggttctcaa tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg
    36901 attcgagtag atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt
    36961 atcatgcaga tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg
    37021 gtcgtattca tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct
    37081 tcatttgccg gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga
    37141 gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac
    37201 catgactaaa ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc
    37261 gcgcttgttc ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg
    37321 gatgaaatat gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac
    37381 tcatgaacat ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg
    37441 cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt
    37501 tttgctgtct atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt
    37561 gttcaaactg atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc
    37621 gtagggataa aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt
    37681 agagagggat ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga
    37741 aagaatagtt gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac
    37801 ctttgctaat cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat
    37861 gatggggaac aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc
    37921 ttatggattg ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt
    37981 agctgtagcc acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat
    38041 caatatcatg tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt
    38101 actagccgga atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt
    38161 atgcccatct ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa
    38221 aaatgatctg cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt
    38281 tatcttggct ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt
    38341 attgatcagt acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat
    38401 cgtcggacgc agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt
    38461 cgaaagagaa aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt
    38521 tgtcggctca gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct
    38581 tcactttttc cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc
    38641 tgtcaatcca aataaattga cattgatcct gatctccatg gtaggaacag tagctataag
    38701 cagcttgcca atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt
    38761 aggagatagt ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt
    38821 attagctgga ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt
    38881 ccaaatggct ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct
    38941 tcaatctttg aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa
    39001 agtcatcttc caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa
    39061 tggcttagca ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg
    39121 aatagcacct ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat
    39181 tatgcttgca gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc
    39241 aaaaagctat gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt
    39301 attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag
    39361 aagcaaagaa taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat
    39421 gattacgctt gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga
    39481 tatagttata aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc
    39541 agtagttaca gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt
    39601 cgttattcca atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga
    39661 agcggatgaa tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc
    39721 tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat
    39781 tttaagtatc catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa
    39841 agatgacatc agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat
    39901 gggacacatg gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat
    39961 acttgcacat atcgatcgaa tccgagataa tacctacgct tatctgattg tagatgattt
    40021 gaacaattta gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt
    40081 aaaaatcaaa ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg
    40141 ttcaagtaaa aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat
    40201 acaaccgcca gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga
    40261 gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc
    40321 agtcattggt acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt
    40381 ccttttattc aaaaaacata ccaaaaatat tccaacatca tcttggactt tctacatgtt
    40441 actaatgagc gtgtaaaaga acgagatggt gtttttattt ctatcaatga agctcttttt
    40501 ctttgcaatc ctcacttcaa tcctctataa tgtcgcatat agtttagtaa atgagtaaac
    40561 tactaattta cttatttact aaaaataaaa ggatgaggag ttaaaataat ggaaatacca
    40621 acaaatttaa aacataaacc agtaatcgtt gtagaagact atgataaagt tgatggaaga
    40681 aatgcattaa atacggatgc aaaagggctt tcactgggcc ttgctcagtg gaatgatcgt
    40741 ggaaaagtcg atatctcagg caaggtatgg cgacatactg gagaaaaatg gtcacgacag
    40801 tcggaagaat taccaatcac acgtatttta gatttagcga tcttagctgc acaaggaagt
    40861 ttatacttcc aagatgctta tcgttatgaa aaattttatg atccagaaaa tccagtcgta
    40921 gacatcattg gattacaagg gaaccgaatg acggtagaag tagataccaa aaatccaaag
    40981 atcgatgaag acatcatttt gtattacgat acactacaaa aagacggaga actgattggg
    41041 gaacgattcc gtattttaaa acgtctttta gatgagctag gatattaata gacagagatg
    41101 atcaaaaggg gaagagaagc atggagaatt tctcactaac agaaaaccga accttttcac
    41161 ggctagaaaa gcaaatgatc tggaagaaac cgaaaagcca tcggacaagt gcagaagaat
    41221 ggcggatcgc ctccgaaatc aaagggaatt ggcaagatcc tgaaatgaaa atcagtaacg
    41281 tacttttgga aggagatgcc gggtcgggga agacacaatt agccaaagca ctatctgctg
    41341 atctgcaact tccctataca aaagtgacct gttttgcaga tatggacaaa tcagatgttt
    41401 tcggagcctt attacctgta gtagattcta ataaggaaca agaccaagaa ttactagaag
    41461 ctatttacca aacagataca cttgaagctg tactgacact gatccagaac cattacggca
    41521 ttgatccttt ttctgctaaa gaaaaattag cagagctagt tcaaaggatc gaagctgatt
    41581 atccagatgc aatccattac agctattacc cttctgaaat cgttcgagcg attgaaaaag
    41641 gatatttatt agagattcaa gagccgactg tcatccgaga tgcctctgtt cttgtggcac
    41701 ttaactcagc gctcgaacca aatgggatgt tgaatttacc gacaggaatc attcgtcgcc
    41761 atccagattg cgtcattgtg atcactacaa atcgaaatta ccaaggaaat cgcccattaa
    41821 atgaatcttt aagagatcgg atgcaacatg cggaaaaaat ggatcttcct gccttagaag
    41881 tcatggcaga acgtgcgatg gcgaaaacta tgatccaaaa acaagaactt ctcttaaaga
    41941 tggcagagat tatccgtttg ttggatgaaa ctgcaaaagc taacgccatc aaaggtgtag
    42001 caggaatgcg ttcttatttt tattgggcaa atacctataa gcaaggacaa gatttactcc
    42061 agtcgattta tccaaaagtt ttgtataaat tatccactga ttcagatgaa ttggcaattc
    42121 ttgaacaagc tttgtctgac agtggattgc tactggaact tcaggacata ttgagaaaag
    42181 aaaaatgggg tcatcctcaa tctgagcata caaaaggaag aaccattagc gccgaagaag
    42241 caaatgaacg aaacatcact gaaaatgctg aagaaattct gcgcttagat gaaaagaaat
    42301 cgttgtctgc tgaagaacca aatgagggag aagagaaaca agaagaaaca atagaaaaaa
    42361 acgagattcc tcctcaaatg caaaaagaac gcagtagcga tatcgaagga aaaaatcaaa
    42421 aagaacaaca aacaggagat gattctcaag aaatgtctgc ttctgacatg gaagcacatg
    42481 ataaggaaat caagaagcaa ctgaataaag aagcgcgaca aatcatgaag gaaacgattc
    42541 atgaaaaaga aggtctcatc gttcaccgac cagctttcca aggaaaaacg ccggaagcac
    42601 gtgagctaca catgcaagta atgccaatag tagaatcact tagccggcaa attttagagt
    42661 tactggataa tgaacaaaca gagacgtacc aaaaaggaaa atacgaaggt cagcggttca
    42721 atgcaagcaa agtagcatat ggtgatttga gaaattttga caaaaagaac ccgccacatg
    42781 agcaaccttc tttagcggta gctgtacgga tcgatgaatc tgggtcaatg atacgggatg
    42841 atcggatcga agcggcaaaa aaagcagcga ttgccattgc cgaattttct aagaaagtcg
    42901 atatcccatt gttgatttac ggagatacag cagaccgttc gtcaagagaa aagacttctt
    42961 tgttttctta taaagaattt gaagacggat ttcaatggtt aaatgaaaaa ttagtcacta
    43021 tgaaaccaag gcaaaacaat cgtgatggtg ctgcacttcg tttagcagca gaaaaattaa
    43081 acagacaatc cgcaacaacc aaactgttgc ttaatattag tgacggtcag ccaaaagcgt
    43141 tgccggatta tacaggcaaa aaagcaaaag aagatatcca gtctgttttg aaagaatatg
    43201 aacgtcaagg aatcctattt gtctccgcag ctattggcca agacaaagaa gaaatcaagt
    43261 cgatctacgg tgaaagtcgt tttgtagata ttactgactt gaatgaattt cccaagcaaa
    43321 tgattcaact gattgcccgc tatctatgat aaaataaagc tcaacgaacg cacttagcag
    43381 attcgttgag cttttcgttt ctagttaccg gtaacaagga tacttctaaa atagaatcgg
    43441 ttataatcaa gagggaccgt taaaggagtg aaacgatatg atacaagaac aaaaatggtg
    43501 gaaagaagca gtaggctatc aaatctatcc tgctagtttc aaagatagca atggcgatgg
    43561 tcaaggagac atcaatggga tccgtgaaaa acttaattat ttgaaagatc ttggtatcgg
    43621 ctttatctgg atcaatccga tctatcaatc tccatttgtc gataatggtt atgacgtaag
    43681 tgactatcaa gatatagaag agagattcgg aacgatggaa gattttgatt tattgctaaa
    43741 agaagcacat gaattaggga tcaaagtgat tatggatctt gtcatcaacc atagttcgga
    43801 tcagcatcaa tggtttgaag aatcgaaaaa atctaaagaa agcccgtatc gcgattatta
    43861 catttgggtc gacggggtag atggcaaaga accgaataat tggacatcga ttttcggcgg
    43921 ttctgcttgg gagtattctc atgaaactgg acagtattat ctgcatgttt ttgcaaaaga
    43981 acaaccagac ttaaattggg aaagcgagaa attgaaagaa gaactattca atatgatccg
    44041 atggtggttg gataaaggga tagatggatt tcgtttagat gcgatttctc atgtaaaaaa
    44101 agatgaatat tcagtaaaag ccacagagaa ccctttctca ccctttcaaa atgtctcagg
    44161 aatcgaagaa catttgaccg atttgaaaca tgtttttgaa gagtacgata tcatgactgt
    44221 cggagaagca agtggtgtca ctgcagaaga aggaccacaa tgggtaggga aagacggtta
    44281 tttcgatatg atattcgaat ttgatcatat ccatatctgg cagcaagaaa aagaagggca
    44341 attagatgtc cttaaattga aacttgcatt aagcgcttgg caaacttcat tggatggtat
    44401 cggatggaac gctttatata tggaaaacca tgatgttcct cgtgccgttt cagtgtttgg
    44461 agatactcgt cccgattttt gggctatgtc tgcaaaagca atcgctatga tgtatttctt
    44521 tttgcaaggt acgccattta tttatcaagg acaggaaatc ggaatgacga atatgccctt
    44581 tgaatcgatc gatcaagttg atgcagtaga ttcgaaaaga ctttataaaa ggctgcttgc
    44641 ggaaggtaag acaagagaag aagcattgga tatcattcgt gaaacaacga gagacaacag
    44701 caggacaccc atgcaatgga cttcggaaca atatgctggt ttttctacac atgaaccttg
    44761 gctgatcact aatccaaata ccaaaacgat caatgtagaa caacaagaat atgaaccaga
    44821 gtcggtcctg caattctata agaacatgat ccgcattaga caaaccaata aaggtctgat
    44881 atacggttct tataaagaat atcttcatga acatccacaa ctttatgtct atgaaagata
    44941 tttagaagat gaacaatatc ttattatggt aaacttaaca gaatcacttg ctgactatga
    45001 acttccaaaa gaagcagatc agtcctggac attactcttg tcaaattcct ctagcggaga
    45061 atttgaagct aaaggcatac tagcacctta tgaagctcgg ttatataaaa caaacaagta
    45121 aaacttcctc gttaaagttc attagttgga ctttgcgagg aagtttttca gattgctctg
    45181 tcaattactc atttcaataa ttcgctctag gaccaccgat atatttagga cgagagcgaa
    45241 gggctgtcac gtgagcttgt ccaagggttt tcaaacgagg tctgacaggt acttggacat
    45301 gtttgatatg cataccgatt gatgtgtcac cgatatcgat accggcttgt ccaactacgt
    45361 gttccacttc gacaggatca ttgaactggt caaaagcagc aacagaacaa gcacctccag
    45421 catgtagtgc aggaacaaca gagacaattt cgaaattttt tgcttctgcc actgttcgtt
    45481 ctactactaa tgctcggttc aaatgttcac aaccttggac agctagcgca atttctttag
    45541 gatccaacaa ctcttttaac gtacggatga tccattgacc aacttccgca ctggaatttt
    45601 ttccaatatg tcctccaacg acttcgcttg tgctgcatcc taaaacaaaa agatcacctt
    45661 ttttcaagtg tgcctcagcc aatatgtcat taactatttc agtcaattgg tcttttaatt
    45721 ctttttctga caacttcatt ttctaaaacc ttctttttca attaatattg cttccgtgct
    45781 tgaatacgtt gaagacttgc agttatagga aaggcaagtc ccattccaat cagattttgg
    45841 aagatgtttc ctggtataga agccactcca gccggccaat catataaaat ccaaccggcg
    45901 ataccatagc cgatgatcat aactaagctt cctaataaaa gccctaatcc ccacatacga
    45961 gtagaagatt tgctgaaaga accagcaaca tatccttgta acccgtggat cactaatgaa
    46021 aaaactgccc actgcggata tcctgaaatc aaatcgatca atagaccact ggaagcacca
    46081 acaactaatc cacccgttgg accataaaga aaagcagcgg tatagatccc ggcctcgcat
    46141 aatgtcacaa atccattcgt agccggtaca gggatcaaaa ttgttaatga aagggcaact
    46201 gtcaacgcaa tcagcatagc atagatcgtt aattgcttag ttttcatttc tcattcctcc
    46261 tttttgaata ccgccagacc agacacttcc ggtatgatcg ttcatcagca cgccattaga
    46321 aatacaatgg gcaacaaatt ttttactttc tgctactgct ttggtaaaat ctgcccctaa
    46381 cacaagattc gctgttattg cagaagaaaa ggcacaaccg gccccgttta ctgttgtagt
    46441 gtcgattttt ttatgagaaa atacctcata atgatcaatt gaatagagaa gatccacagc
    46501 ttcatctcca gccatccttg tgccgccttt gatcacaatg ttttttgttc ctgaagcagt
    46561 gagttcttta gcgagatgga tcatttctgt taacgaagag ggaagagctt gaccagaaag
    46621 taaagcagct tctgccaaat taggcgtaat catcgtaaca agtggaaaaa gttcttcgat
    46681 gagctgcgaa atatacaatt gattgatcac agcttcagtt tctttgaatg ccaaaacagg
    46741 gtctaaaatg attgggatat tttgttttgt ttttatgaag tctcgaacaa ccatgattgc
    46801 ttcacttgaa ttcaataacc caattttgat accatctaat gaagtcattt tatcaatcga
    46861 aactaactgt cgtttaagca attccaaaga tatagattcc agcataaatc gctcattaat
    46921 atctagtgcc cctatagaag tcaaggcgct tagtccgaat agaccaaaat tctcaaaagt
    46981 ttttaaatct gtttggattc ctccaccagc aaatggatca gaaccgccaa ttgtcaagat
    47041 tcttttcata acattttcct ccatgaataa gagtataacg aaattttcaa aaaagaaaaa
    47101 cagccaattg gcatattttt atctcatcca atttaaaaaa acaaaaagaa aagaaagacc
    47161 aaaatggatc tttctttcct taaatagtta ggacttttgt gattttaatt ctttctcgat
    47221 ttgaagccgt tgcactttca ttgtagctgt tcgcgggatt gcatcaaaat cacggatgat
    47281 agggacgttc aagcgaggaa gttcatggac ttgctcccac catcgattcc aatccatttc
    47341 tttatcagga gctaaagcaa taatcggttg tggagaatga tttttatccc ttacaatcac
    47401 tacttctgcc aaaaaatcta acgaatctaa taaaaagtct tcaatagcta atgtactatt
    47461 gatcgtttcg atcaaatcca cttgccgatc tttcaaaaat aggtggccat gcttatccat
    47521 aaaaccgtaa tctccgctat cccaccaatc gccataaaca ttttcctgga accgtgcatc
    47581 ttccttgtaa taagtcaaag cccgtccttt agacagtaat tgtatatgac catctgtgtt
    47641 ttcaggtagg acattcccaa ctgaatctgt aatccgagcg cttgtcatgt cttccagtcc
    47701 aacgcccata tctcttgcat cacttgtttt caaactctct agtgtatgag ccttaagaat
    47761 cattggtccg cattcacttt ggccatatac ttgaagaaat accgcatcct gtgccatcga
    47821 cgttcgcaaa aatgtcttca ttgtctggtt gtttatagca tcaaaagtgg agtgatagta
    47881 tcggataccc gaaaaagctt ctggatgctc cttagcagta aaacgccact gaacaaagtt
    47941 attcggatgg gtctctagtg caattggccg gtgagcttcc aacatatgaa caacttttga
    48001 actttgtgcg tttgctaaag gcatcatagg aaatcccatg gacatcaaag aagagatacc
    48061 aatattgaat ctggaatgca ctggagagat gtgaaaagcg attaatttct tttccgaaat
    48121 ttttgtgaaa actgtttttt gccatttcgt ccgccatccc atagaatgag cagaatgaca
    48181 aataagttta gggataccag ttgtaccaga agtatgggtc atataagaaa tttcattttt
    48241 gtctaaaaat gattgagaaa cagacaaagc aggttgttcc attaaataaa gaataggaa
//
LOCUS       DAYYCQ010000012        46084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000012 DAYYCQ010000000
VERSION     DAYYCQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.012"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..939
                     /locus_tag="ACHAYF_000717"
     CDS             58..939
                     /locus_tag="ACHAYF_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8578179.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            952..2562
                     /locus_tag="ACHAYF_000718"
     CDS             952..2562
                     /locus_tag="ACHAYF_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578180.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2639..3298)
                     /locus_tag="ACHAYF_000719"
     CDS             complement(2639..3298)
                     /locus_tag="ACHAYF_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8578181.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3581..4246)
                     /locus_tag="ACHAYF_000720"
     CDS             complement(3581..4246)
                     /locus_tag="ACHAYF_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8578182.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4393..4998)
                     /gene="dhaL"
                     /locus_tag="ACHAYF_000721"
     CDS             complement(4393..4998)
                     /gene="dhaL"
                     /locus_tag="ACHAYF_000721"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8578183.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5026..6036)
                     /gene="dhaK"
                     /locus_tag="ACHAYF_000722"
     CDS             complement(5026..6036)
                     /gene="dhaK"
                     /locus_tag="ACHAYF_000722"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8578184.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6011..6406)
                     /gene="dhaM"
                     /locus_tag="ACHAYF_000723"
     CDS             complement(6011..6406)
                     /gene="dhaM"
                     /locus_tag="ACHAYF_000723"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8578185.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6560..6880
                     /locus_tag="ACHAYF_000724"
     CDS             6560..6880
                     /locus_tag="ACHAYF_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8578186.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6992..7849)
                     /locus_tag="ACHAYF_000725"
     CDS             complement(6992..7849)
                     /locus_tag="ACHAYF_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8578187.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7957..9261)
                     /locus_tag="ACHAYF_000726"
     CDS             complement(7957..9261)
                     /locus_tag="ACHAYF_000726"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8578188.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9258..9767)
                     /locus_tag="ACHAYF_000727"
     CDS             complement(9258..9767)
                     /locus_tag="ACHAYF_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578189.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9942..12077)
                     /locus_tag="ACHAYF_000728"
     CDS             complement(9942..12077)
                     /locus_tag="ACHAYF_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8578190.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12160..13815)
                     /locus_tag="ACHAYF_000729"
     CDS             complement(12160..13815)
                     /locus_tag="ACHAYF_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8578191.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13817..14131)
                     /locus_tag="ACHAYF_000730"
     CDS             complement(13817..14131)
                     /locus_tag="ACHAYF_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8578192.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14301..14561
                     /locus_tag="ACHAYF_000731"
     CDS             14301..14561
                     /locus_tag="ACHAYF_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8578193.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14575..14889
                     /locus_tag="ACHAYF_000732"
     CDS             14575..14889
                     /locus_tag="ACHAYF_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8578194.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15619..16173)
                     /locus_tag="ACHAYF_000733"
     CDS             complement(15619..16173)
                     /locus_tag="ACHAYF_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8578195.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16299..18228)
                     /locus_tag="ACHAYF_000734"
                     /pseudo
     CDS             complement(16299..18228)
                     /locus_tag="ACHAYF_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18534..19250)
                     /locus_tag="ACHAYF_000735"
     CDS             complement(18534..19250)
                     /locus_tag="ACHAYF_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8578196.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19251..21074)
                     /gene="glpO"
                     /locus_tag="ACHAYF_000736"
     CDS             complement(19251..21074)
                     /gene="glpO"
                     /locus_tag="ACHAYF_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8578197.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21088..22584)
                     /gene="glpK"
                     /locus_tag="ACHAYF_000737"
     CDS             complement(21088..22584)
                     /gene="glpK"
                     /locus_tag="ACHAYF_000737"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8578198.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22720..24180)
                     /locus_tag="ACHAYF_000738"
     CDS             complement(22720..24180)
                     /locus_tag="ACHAYF_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578199.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24170..25465)
                     /locus_tag="ACHAYF_000739"
     CDS             complement(24170..25465)
                     /locus_tag="ACHAYF_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8578200.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25797..26474)
                     /locus_tag="ACHAYF_000740"
     CDS             complement(25797..26474)
                     /locus_tag="ACHAYF_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8578201.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26504..27595)
                     /locus_tag="ACHAYF_000741"
     CDS             complement(26504..27595)
                     /locus_tag="ACHAYF_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8578202.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27696..28130)
                     /locus_tag="ACHAYF_000742"
     CDS             complement(27696..28130)
                     /locus_tag="ACHAYF_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8578203.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28714..30171)
                     /locus_tag="ACHAYF_000743"
     CDS             complement(28714..30171)
                     /locus_tag="ACHAYF_000743"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8578204.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30168..32063)
                     /locus_tag="ACHAYF_000744"
     CDS             complement(30168..32063)
                     /locus_tag="ACHAYF_000744"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8578205.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32121..33896)
                     /locus_tag="ACHAYF_000745"
     CDS             complement(32121..33896)
                     /locus_tag="ACHAYF_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8578206.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33946..35442)
                     /locus_tag="ACHAYF_000746"
     CDS             complement(33946..35442)
                     /locus_tag="ACHAYF_000746"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8578207.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35636..37258)
                     /locus_tag="ACHAYF_000747"
     CDS             complement(35636..37258)
                     /locus_tag="ACHAYF_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8578208.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37586..38899)
                     /locus_tag="ACHAYF_000748"
     CDS             complement(37586..38899)
                     /locus_tag="ACHAYF_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8578209.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39144..39368)
                     /locus_tag="ACHAYF_000749"
     CDS             complement(39144..39368)
                     /locus_tag="ACHAYF_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578210.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39388..39810)
                     /locus_tag="ACHAYF_000750"
     CDS             complement(39388..39810)
                     /locus_tag="ACHAYF_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8578211.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39952..40662)
                     /locus_tag="ACHAYF_000751"
     CDS             complement(39952..40662)
                     /locus_tag="ACHAYF_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8578212.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40680..41177)
                     /locus_tag="ACHAYF_000752"
     CDS             complement(40680..41177)
                     /locus_tag="ACHAYF_000752"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8578213.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41214..41666)
                     /locus_tag="ACHAYF_000753"
     CDS             complement(41214..41666)
                     /locus_tag="ACHAYF_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8578214.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41801..42406)
                     /locus_tag="ACHAYF_000754"
     CDS             complement(41801..42406)
                     /locus_tag="ACHAYF_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8578215.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42856..43476)
                     /locus_tag="ACHAYF_000755"
     CDS             complement(42856..43476)
                     /locus_tag="ACHAYF_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8578216.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43478..44347)
                     /locus_tag="ACHAYF_000756"
     CDS             complement(43478..44347)
                     /locus_tag="ACHAYF_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8578217.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44370..44777)
                     /locus_tag="ACHAYF_000757"
     CDS             complement(44370..44777)
                     /locus_tag="ACHAYF_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8578218.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44796..>46084)
                     /locus_tag="ACHAYF_000758"
     CDS             complement(44796..>46084)
                     /locus_tag="ACHAYF_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8578219.1"
                     /translation="GVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKTL
                     LGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALTF
                     LAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGACF
                     AIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLPT
                     HFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVYV
                     LLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTIIA
                     MFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALLV
                     TIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12933 a         9838 c         7890 g        15423 t
ORIGIN      
        1 tattaaacta tttaatcttg tttagtatat atttttagaa aaaggagtgg ggtttttatg
       61 gcagaaatcg tcaaagtcac acatttgaaa aagagctttg gtcgtttcga agctttaaaa
      121 gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat ttatcggacc aaatggttct
      181 ggaaaatcca cgacgattcg gacactttta gggattataa aaaaagacgg cggaacggca
      241 acgatttttg ggcaagatgt atgggaagat agtttatcta tccatgaacg tctttcttat
      301 gttccaggcg atatctcttt gtggggaaat ttgactggag gagcgatcat cgatctcttc
      361 atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg aactgattca acgtttctct
      421 cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg
      481 attgctgctt tggctgttga ttcggatctc tatttatttg atgagcctac ttctggttta
      541 gatcctttga tggaaacgat tttccaagaa gaagttgaaa aattgaaaga acagggcaaa
      601 tccatcctac tttcttctca tattctcagt gaagtcgagc gactagcgga taaagtcgtg
      661 atcatacgcg aaggaacact cgatgaagaa ggaacactcg atgaattgcg tcatttgact
      721 cgttcgacca tcactataga aacagaagca gagaatagtc acttagcaca attgaacggt
      781 gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg ctgctgataa cgaagctttc
      841 gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg
      901 actcttgagg atttattcat gagacattat gaaggatgag gaggaagcga aatgactgat
      961 aaattttcac gctggtccac ccttttttct caaaacctta gccgtgactg gaaaaagatc
     1021 ctgatatggc tagtcggtgt cactgctttt tctggtggtt ttgtccctgc gtttgaagaa
     1081 ttgacttccg gacaaggatt agctggatta tacgaaacct tgcaaaaccc ggcaatgatt
     1141 gcaatggtcg gaccaactcc agcttcgaat gctgctgagt atactttagg agcgatgtat
     1201 gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg tgatctctgt tttacacgtg
     1261 gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg aacttattcg ttcctttcga
     1321 gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa gtattctgat caatttgctt
     1381 ttagcaatat cgatcagctg tacgatgatt tttttccaag cggattcgat cactgcagaa
     1441 ggtgcttggc tttttgggct atctgtaggt agtatgggga ttttggggag tgccgttgca
     1501 ctgtttgctg cacaactgat gccaagttca gccagttcga atggtctgtc tttagcgttt
     1561 ttaggtggac tttaccttct acgagcaata acagatgtag ctgattcctc cctttcgatg
     1621 tggaatcctt taggctggtc ttatttgacc tatccattta caactaacaa ctggattcct
     1681 ttgctttatt tgtttttgtt cagtctaatc ttgataatca gttcatttgt tttagaggaa
     1741 catcgagatt tgaacaccgg ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt
     1801 tctcttttat ccttgcgtgg tttattattc tatctaaata aagggatgac aatcagttgg
     1861 cttatcactt ttggtttgat gggggcggca tacggctcta tctacgggaa tatgcaacag
     1921 tttttagaaa gtaacgactt aatgaaacag atgtttatcc aatctggcgt ctcaattgaa
     1981 aaaaacttta caagcacgat catggtagtc ttgatttgtc tagctgctat tttaccaatt
     2041 gctagcatca atcgtttatt tacagaagaa acccacggac gtatgaatca gctgttcgca
     2101 acaaaaattt ctaggaacca actttattgg accaatatgt tactagctat tctatttgct
     2161 atattggcaa tcctgattag ttctggtgca ctagggcttt ctgctttggc ttcgatgaag
     2221 gatgcgaaag acatggatct attgatgttt cttaaagccg ggttcaattt cctgcctgcc
     2281 gttttagtgt ttatcggaat tagtggcttt ctattcggat ggcttcctcg aatcggtaaa
     2341 cttgcttata tctacttagc ttactccttt gcactcaatt actttggcgg aattttggat
     2401 ttaccagaat ggttttccaa aacagctgtc cctagctggg ttccacggat gccaaacgat
     2461 tcttttgatc tttccgtgtt ccttacgtta tcatgtatca gccttgcact gttcgtcctt
     2521 ggttctattg gttataggcg aagagactta ctagaaggat aataaaaaag catgtacaat
     2581 ttcatgctag cctccaaatc tgggtcaaaa atccaacatt tggaggtcat ttttttattt
     2641 acaagtcttc taactgctct ttatcaatga tccagacttc tcgattcttt ctttttacat
     2701 aaccattctc ctctaataat ttaaattttc tagataaagt ttcgggggtc gtaccgataa
     2761 atgcagcaag ttctttcatt ttcattggca ggaaaaagtg atccttccct gtcgctttag
     2821 ctaagtcaag cagatagaga gcgactcgtt cttctacttt ttccatcatc aaaaaagttg
     2881 tctgctgctg tacttttgtt aatttctcaa cgttgattgc taaaagtttc agactaaggg
     2941 cgggataatg cagtaagagt tccgagaaat ctttttgtct caatatgcaa atttccgttt
     3001 tttctagtgc ttcgccaaat aaagactcat ttggaacgcc tagcaactgg ctctctccct
     3061 catatccgcc cggctctatt actcgtaaaa gctgttcctt tccgctagca gataattgat
     3121 acactttcat actgccggag gccacgatca ctaattgagg ttctccattc ggcgcaagaa
     3181 tctgttctcc tttttccaca tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt
     3241 gctgtactaa gtgattgaac aaaggaacaa atgaaacaca taaatgatca tgactcataa
     3301 tctctcctcc ttctaaacag ctatttctaa taaagaaaca taacttccct tattccaact
     3361 gataattttc ttttatcata gcataaacgt caagcgaacc agataccagc aaatagagtg
     3421 gatttctttt tgtcccttgc tttattttcc ttcttttgag accttaagta attgattcct
     3481 atcaattttt taaatattta ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg
     3541 agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt ctattgaaat ttttccgcac
     3601 ctagcttttg atatagtggt gctagttctt gtaaggtttc agcggttatt tgctcaacgt
     3661 gttcgatttc ttgcggatag aagaatagtt tacccatttg aaaggttcgg tctctcatcg
     3721 cttttttcaa ctcttcttgt ttctttgtat cggacaaata gtcagacaaa gctaagtggt
     3781 cgtcaagttc atgttctggt tgcggccata tgtaataatc cttgatatcg actgttttgc
     3841 tccactcttt cagtatcgca agcaactggt tatacgctgc aatgctacca tatcgttctt
     3901 cgcttttata atgttggtac atcaaataga tttggtattg tttttgattc aacaaaactg
     3961 ctagacaggc attctcgtct tgacgagatt cgcttcggta cgcgctccaa aaatgactgc
     4021 gtaaattcca gccattcgtc cagctttcga ttttaggttt gctcatgccg tacatatcag
     4081 gcaaaagtgc agctgtctgg agctgaatct ctttccattt ttgccagtgt tctttatatt
     4141 ctttcttgat ttgttcaatc ttatctgttg gatggctctc tttgagctgt ttgaatgcgt
     4201 agattggtcg atcaaacaac tgataatctg attttccaag ttccattgtt tcgcctcttt
     4261 ccgttatctt ttgtattcta cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa
     4321 gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca
     4381 gttttattga acttaaggtt gattgatcac atctaaaagc gttgaaaata aaatggcact
     4441 agaagcagct cctgggtcga tatgtccgat tgcacgctct cctagatagg aagctcgtcc
     4501 ttttttagct accaagactt tggtgtgttc ttttgcctgg tcaatgattt cttttgttaa
     4561 attccctgcc ttcaatgcct ctgtgactgg cagccaaaca tctaacatcg ttttatcgtc
     4621 agcttctgcc tttcctcggc tttgaatacc cgctgttccc tgctcgatca cttcgcctaa
     4681 tttttcttga gaatcaatag aatcgacctc ttttgtcgct ttcatcatgt tcatgaatgc
     4741 agatccgtat aacggaccag aagccccacc gactttcgat atcatcgcca tagagaatac
     4801 tttgaatgta tcgctgattg tttcaggatg atcggcttga aaagcttctt cataagctga
     4861 aacccctctt cccatgttat ttccatggtc tccatcgcca atcggggtat ctagatcact
     4921 gagatattgt ttgttctgat tgattgcctc tgcaaattgc gtgagccatt cttggatttc
     4981 ttgaacggtt agtttcatat tatcaccctt ttatcttgag attttttacc atgaaatcgt
     5041 ctgtacattg ctttctaaag aatctttcca ttgtgtctcc gtcaagtcga tcagtgttag
     5101 agaaagacct tgcatatcta gtgaggtcat aaaattaccg acttttcgga aaactacatt
     5161 gacgcctctt tcttccaaca gagctaaaac atcattcatg aacacgaact gttccatcaa
     5221 aggcgtcccg cccattccgt tgactagtac acctgcttct tttggtaaat tcgagtaatc
     5281 atctaaaatc ttagtaacaa gttcctttgc cagtacttta gaaggctgca tcttttctcg
     5341 acgatagcct ggctcaccgt ggataccaac accatactcg atctcatctt ctcctagttc
     5401 gaatcctggt ttccctactt ctggtacagt tgctgctcga agcgctagac cgatcgtctt
     5461 agttgcctgt acgacctttt ctcccagttc tttcaactca gctagggaag caccgtttct
     5521 tgctgcatcg cccacgattt tgtgtacaag gacagtacct gcaactccgc gttttccagc
     5581 agtatacgta ctattttcaa cggcgatatc atcatctacg atcacacttt ctacttcaat
     5641 atcgtccata gcagctaatt cctttgccat atcgaagttc aatgcatccc ctgtataatt
     5701 cttaacgatc agcaaaactc cttttccact atcggcttcc ttaatagccg tctgaatctg
     5761 atctggagtt ggagaagtaa acacatcacc aagcaccgct gcacttaaca tcccttcccc
     5821 tacaaacccg gcatgggatg gttcatgtcc gcttcctcca ccagaaacca aaccaacttg
     5881 ttccgctttc tgattcttgg ctacaacacg ggaagattct acacgatgaa caagttctgg
     5941 atagcttttt actaatcctt ccagcatttc ttcgacaata ttgcttggat cattcatgat
     6001 ttttttcaat ttattcccat cctttctttc cggcattttg tgcttctgtt aagatttgct
     6061 gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa agctccttcc actaaaggcg
     6121 catccaccag atgataacgt tgctgctgct cttcttccaa catgtctttt gccagttccg
     6181 agttcaatac cgcgctaccg atatccgcaa aaacaaaata actagctgca tcgcttgatt
     6241 cattgactgc ttctacgatt ttcatcgggt ctgatccgat ctcgccattt attccgccga
     6301 gagaaaaaat ttgcacctct tcggattttt gcatctgctc aatcatttct ttaataccat
     6361 ctgttacttt ttgactatga gaaataagca aaatactttt tttcacaaga atcttccttt
     6421 cttttacata gttgctaacg attggtttcg ctttcattaa tgaacaattc aagtatataa
     6481 taaatttcta attttgggaa aatatgtgta gtgcttcttg aagtttgtta tactttaatt
     6541 aaaagagagg agtgatcgaa tgattacaac aacaacgaac agtgtagagg gaaaagccgt
     6601 agtagcttat aaagggatcg tttttggtga ggtcattact gggatccatg catttaaaga
     6661 cttggaggcg gggttgcgaa atatctttgg cggccgctca aaaagctatg aaaacgaact
     6721 aatgggtgca agagaagaag cacttgccga aatggccgaa cgtgcaaaag cattaggagc
     6781 tgatgcgatc attggcgtaa aaatggacta tgaagtatta ggcagcgaca atggcatgct
     6841 gatggtcaca tgtagtggaa cagctgtctc cacgagataa gtaggggaca tctgttttaa
     6901 taactaaaga gagatgggac atgtagtggc actcaagaat tagatttttc agtctaactt
     6961 cgaggtactc taccttccca tcctctcttt tttatttctt tttgcggatg attccgtaag
     7021 ctgctgcgac taacacaccc gcccctgcta aggaataact gacagaagct gttttttcat
     7081 ttgtttttgg caaagtctga cttgttgatt tggatggctc tgtcgtttgc tgaactgttt
     7141 ctgtcgtgct tccagaggtc actgattttt ctcctgccga atgcaaaata tcttttattc
     7201 gttggaaact agctgctaag tcagcttttt ttgcttcaaa atcattagca actgtttgtg
     7261 cttcgctgat ctttccgtgg attgcttgac tggccgctgt gatatccgct tgagtttgac
     7321 ttagctttcc agaagctgtt tgagcaacct gatttaattg ctgattgaat tcttttgctt
     7381 gttggatctt cccgtcaaga tctgtttgga cagcttccgc ttttgctttc gcttcggcaa
     7441 gctttttctc aagttgtgca cggtcatttc ccactttatt tttaagatca gctaaatctt
     7501 gatcgattgc tgcttggtct tctttcaatt tgttttttgc agcttctaat gcttcttcga
     7561 gttcttttct tttattttcc aagtcttgca aatgatcgat ttggtcagaa gaggtagaac
     7621 tatttattga tgtatcttct gaagatgaaa tcgtttctcc tgaattatct gtcggtacat
     7681 tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc agaagagctt atggttgttg
     7741 aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa
     7801 tactggaaac agcaattggc cagattaacg gcttcattct ttttttcatc acttttccca
     7861 tccttactac tttttaacta atttcttatt ataagcaaaa atgtttggaa gaaccataaa
     7921 cacaacaggc tgtttgtcta aaaaacggtg catcttttat ttttttgtac gttccgggct
     7981 tttccatgtc ccgcttccat aacctaaaat cgttttgact gctggaatga aagtcgtcac
     8041 gatcgttatc gcgttcacta tccagaacaa aagcatatat agtggtgcga aaagaaggta
     8101 tttcaatttt cttcgtcgat catctacaat cagcgaagct agcaactgga aaaatcctgc
     8161 gaccatttca aagcagatga acacaaaact gaatgtaaaa gtaatataaa tctgttcgta
     8221 gtttccttga tatacataat gcccgattaa tccagcaaaa agaactacgg aaatacaaaa
     8281 gaatattgac cagacaatac tcaatgtttg atcaatgaag atgatcgttc ttccaatatg
     8341 tttaaaagga tggagcatca cttttttgaa attcgtcagc cagacttctg ttccaccttt
     8401 tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc acaggtactt ccatgaaaaa
     8461 gataatctca ggagcaaaaa cagataacca gtcatttaac tgatgatccc aagcaatact
     8521 gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg
     8581 atacatcgta tttgctccac tgtacgcata aagaccaccg aaaacagctg actgtgttcg
     8641 tttaatgatg cccacgatac ttgagaattc aaccgtttga gattttgcaa tcaatttggt
     8701 acgattttgg acatccatat ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat
     8761 gaaataattc acatacctaa tcagtgcatc tggctccggt accgtgtctg catcattgct
     8821 taaaatcaat ttcccttttg caaaggcaag tccgatatta aacgcgtggg cttttccttt
     8881 atttttttcg atccgaacca cacgaagatt agcgtatttt ttcatcagtc tagctaatat
     8941 ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat acctcatagt tgtgataatt
     9001 caatttcgtc atcaaatact cgattgtata ttcgatcaca atttcttcat tatgggctgg
     9061 caccatgatc gtgatgaacg gttcgacact caagggaaca tctacccatt ctttttgctt
     9121 atgtttgtat aaaaacacat aacaccagac accaataaac catacaaatc cgcctaaaat
     9181 cggataagaa acgagaaaaa aatacataat agacaaacaa gtatctaaga ataaattacc
     9241 tgtatgggtg attgagatca tttcagttcc accccatttt ctttatacaa atctgcaatg
     9301 aatgtcgtat ctagattttt ctcttcggag acagaataaa aacggaccgt ttctctctct
     9361 tcttttgttc caaaacgttc cgtatacact tcttcaagta attcttgtct ttttttcaat
     9421 ttttcatctt catattcaaa agatttattt acttttttgt atagatttcg attgttccac
     9481 ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt
     9541 aagaaataca gcattttctt ttctgataaa aaagcgtaca aggaaaaatc cttggctctt
     9601 tctggtctcg ttaatgggaa aaagacccat ataaaaggaa gaacaacgat catccatcct
     9661 aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat taccttttga aaaatactta
     9721 tctttcacca aaattgatgc aacttcttct cttgttactt ttttcatatc ctcacctcac
     9781 gtttttttgt tacgacgtag gagtttacca tacatttcat ttcttttcac ataaagtgtt
     9841 cctaaatttt tcctaagaaa aagaacaaaa catttctcat cagcaaatga aaaatatttt
     9901 gttcttttta tcagttgttc tttttatttt ttatctctta attatgttgc tctttccaat
     9961 acgacaatgc ttttcattcc tctagatatc gccgtgtaca tttgtctgcg tcttctttgc
    10021 tcatctccat cgttttcaaa tggatataag attacatgat caaattccag tccttttgct
    10081 agactcaaag gaatgatttg gcagttttct aatccatatg aatttttttt aatcgattct
    10141 tgtagcgttt gggtttcctc ttctgtttgg gtgataactg ccgctttttc tccttttaga
    10201 gaaaccaaaa tctcagctag tattttcaac aattcttcag aggatcgaaa agagcggtat
    10261 tcgggttctt ttccttgcgg tcggatcgat acgatgtcga tcttctcttg atccacagca
    10321 taggttttga aaagctctgt gattgctccg ctagaacggt aactatttct aagatcatac
    10381 cgatggatcg gcagatggta gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa
    10441 gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa agtgagtttt tggaaataat
    10501 tcgatcaata gacctaattg tgccagggta taatcttgta cttcatcaat caatagatag
    10561 cgaaatgctg gtacatttac cttttcaaca agtaaatgac gaatagctaa tataatgact
    10621 gcttcatcaa cagtacgtgt gggttgccaa gcataagctg agccatatcg tttttcataa
    10681 attctttcaa acaactgatg ctcatccacc catgccattt cttcgatttg tctggttatt
    10741 tttcgatatt ttttttgtaa aagtttcctt gcataagcta caatactttg ttccgaatca
    10801 tcagaaatca atttaccgaa taattccaac tgcctatctt ctgtcaacga gagtacctga
    10861 ttctgcacag caggactttt tgcttgtttc aaaagatgat tcttccattc gctcatcagt
    10921 gcctttttcg ttgcttgaag cctatcgatc aatttaggat gagcaggaac tttctcatat
    10981 attttttcga tcgttttttt agaaaggact gtttttccat ttttagtaag tccacggata
    11041 aaattcggat gatctaaaaa caaaggatct gattgtttta gattatcgat aaactttttg
    11101 ctacgcaaac gttctgtctg ctccgataca ttttctcccg tgatacgttt gaaataagcc
    11161 tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca gctgtatgat ggttagattc
    11221 agtggatttc tttcacctaa tgatggtagg acattcgaaa tatagtcgat aaaccgatga
    11281 ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat ctcgatataa atagagcaga
    11341 tacgcaatac gctgcatgat agcagaggtt ttaccacttc ctgctacgcc atttaccaag
    11401 atattttgac ttttcacatc ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg
    11461 atagccttca tttcattggt ctcatcttgg cctaagactt ctaataatac gtcatcttga
    11521 atagctacag acgtatcaaa aaacttgacc aaacgatctc tttccaaaag aaattgtcgt
    11581 ctttctttaa taaccgtatc gattttattt tgacgaaccg tataagaaga tggaccaatc
    11641 atgttattgt aaaaaagttc ggcaattggt gaacgccaat cataaacaag attttcatct
    11701 gcatcatctg tgaaatttgc tgtaccgata taaaaggatt cttttggctc attatccaga
    11761 aagtctacaa cgacttttcc aaagtaggga cttttcaata aacgttcgat ctttgctagc
    11821 tgattttctg cgtttttctg tttgatattc atctgatcga tttctctatt tttcatttca
    11881 atcatcgaaa aagtatccag gttatctaag taactgtcaa aattcaatcg aagctcgcca
    11941 gccatttctt tcaacgactg gtttccttct atttttgccg tatccatcga ttcccgcagt
    12001 tcgttttttt tatcaattag cttttgatac acgttcttca gatatgcttc ttctaactgt
    12061 ttttgtgtgt tgaccataca cattctcctc tttttttaaa gtggtggaac cgattagttt
    12121 acctctttta agagtcgaat gcaagctttt taacgagtat catttgttag gatatatcag
    12181 ttcttcaggt cggacagttt gatataaaga aaatgcacca tcaaggtttt ttacgtggta
    12241 gccagactgc ttcaagattc tttccgctag atagctacgt aatccgctat gacagctgac
    12301 gatatattct tgttggctgt ctaattcatt taatcggtcc cgaagttcat tcaaagggat
    12361 atgtttggct tccttgaatt tgcccttttc caattcttct gctgttcgca catctaataa
    12421 aatttttcct tttgctagtt ctgtcggcaa ttcatgccat tgtacggttt cactaagccc
    12481 ttctgcaatg ttcattgctg catatcctag catgtttact ggatctttgg ctgagccaaa
    12541 tggcggtgca tacgtcaatt ctaattccgg taaatcgaag atcgtcaaat gccctttgat
    12601 tgctgttgct aaaatatcga tccgtttatc cacacctttt gccccaacac cttgtgcacc
    12661 ataaatctcg ccagtctctg gatggaagat caacttcagt aaaatatctg ttgcttctgg
    12721 ataatagctt gcatgatctt ttcctgatac atgaatgact tcaaaagaca gttcgttttc
    12781 tttggctgtt ctttcactta aaccggtgga tgcggcagtc attccaaacg ttcgtataat
    12841 tgctgttcca ataccgcctt gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg
    12901 tctaccttga cgattggctg gtgaagcaag agaaataagt gcatcttttc cagtgatttg
    12961 ctgttttaca acgatagcat cccccaccgc ataaatatca ggaatattcg tttgataacg
    13021 ttcgtctact aggatacccc cgcgtatgcc taattcgatt ccagcggtca ctgctagctg
    13081 gttatcagga cgaacaccaa tggataaaat aactaaatca gcagggatgg tacgcccatc
    13141 atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg acagcatgag aagtaatcac
    13201 ttctactcct tgatgagaca attctttttc tataaaagct gccatttctt catccagtgt
    13261 tggaagaata tgtggtgctt gttcgatcac catgacttct aaacctcttc tttttaggtt
    13321 ttctgccatt tccaatccga tgaatcctgc accaacgatc acggcacgct ttggctgttt
    13381 ttctaatgca tgcatcactt tatcgatatc tggtacattt ctaatagaaa aaacattgtc
    13441 ggcttctgtt atccctggaa tcgatggaac aactggtttt gcaccaggag ataaaaccaa
    13501 tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg acttctaccg ttttgttttc
    13561 gggaaagatg gctgtcactt catgatttgg acgcacatct aaacaaaaac gagcggataa
    13621 tgattctggc gtttggacaa gaagattttc ccgttcactg atttctcccg aaagataata
    13681 aggcaagccg caatttgcaa aagaaacata cggtcctttt tcaaaaacaa cgatttctgc
    13741 atcttccatt aatcggcgaa gccgtgtagc agcagacatt ccacctgcta cacctccgac
    13801 aataacgatt ttcatcttat ttccctccta ctgttcgatc aaaccattga ttcatgccac
    13861 cacgtacatt cactacatca taaccagatt tctttaattc tttagctgct tgtttactcc
    13921 tcatccctga ttgacaaatc acatagacgg tcttttcttt gtctccatca tatctgttta
    13981 tactttgtaa tggaacattt ttagctcctt ttatatgccc gccccggtat tctgcaggtg
    14041 ttctaacatc aagtaacgct ggattttcca aaagcttttc ctttaattct gggactgtta
    14101 tcgattttat tgattgaaat agcaaattca ttttctgcac ctcatttaca tttttataaa
    14161 tacctcatgg ggtatattaa acaggagctt tattaatatg tcaaccagaa tgactcttca
    14221 tttttttcac tactttttat atttgcataa aataccgata aggggtatta tctaattata
    14281 aaaatagagg agaatgtgtt atggagattg ataataaaaa tatcctgaac cgtttgaaac
    14341 gtacagaggg acaaattcgt ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg
    14401 atgtcatcac tcaattaagc gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg
    14461 ttgcagataa tctgaaaaat tgctttgaga aacctgatag taatccagaa gaacaagcgg
    14521 ctaaactaga acaagcgatc aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa
    14581 gtcgttttcc atattgatga attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac
    14641 ctgataaaag cttctccaga aacgacgatt gtcgtatctg taaatggcat cgcgattacc
    14701 ggttatctag actctgccaa tgcagaattc ttagatttgc aaggagtcgt ttttcacgct
    14761 tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt ctttacctga acaagtaatc
    14821 gttgtaccag caggtgtcct tgatttagtt gaactgcaat cccaaggtta tgcttatatc
    14881 aagccttagt cagtagaggt tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt
    14941 tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca
    15001 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcgttt ggttgggctt
    15061 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa atcagcggtt
    15121 tgacctaagt attagagaaa aaaacgggaa aatagctcct catattttgc cgttttttga
    15181 gctaataccg aaggtcagct gattgaacac cgtttaagga agagattgtg aaaaagtcgc
    15241 ttagtttcga gtattaagaa aaattttgtg aaaatggatt tctacatttt tgcataaatt
    15301 tggcttaata ccgagaaact ggcttttgaa taccgtttaa ggaagaggtt gtaaaatcag
    15361 cggtttgacc tgagtattag agaaacaaac ggaaaaatag ctgctcatat tttgccgttt
    15421 tttgagctaa taccgaaggt cagctgattg aacaccgttt accaagaggt tgtgagccta
    15481 ccatttaact ccgaatcaca agaaataatt tcaaaaacaa cttctcctgt ttttggttcg
    15541 gttggcttat tatcgagaag tagctactgg gagtctattt atcaaaaaaa ggaatgcgac
    15601 ataggtcaca cccctttttt attttctttg aaacatcttt cggtactcga cagcatacaa
    15661 gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa atgactctca cccacagcgt
    15721 tggtaattct gggaaaagca gcaaaaggat catgccaaca tagatgacag cagtcgctac
    15781 tttgccatac cacttcgctc cacttaattt gatattttta gatagcccga ttaatccagc
    15841 tacaaacata aagccttctt ttatcaagaa tagaccaaac aacagccaaa gccacggtcg
    15901 ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag
    15961 caccttccct aagtctgtta cctgattata tctccgagca atgaccccgt ctagataatc
    16021 agacaaacct gagacaacaa tgaccgctcc tgctatataa aatgactgtc tcagcacaaa
    16081 ataaagataa atcggcagca aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg
    16141 tatttcttta cgccagtctg tgcttaattt cattttatcg ccctacctcc tcagcactct
    16201 tttcttttta ttttagctta cacggggaat aaaaccaatg atttcactgt ttttattttt
    16261 ctttacagac actgatatta tgagctaatt tgagataatc acttgctgtt ttcatcgttt
    16321 actcttcttt cttcctgtcg tttgcgcatt tcttttacta gtactttatg agcttttttt
    16381 gatcctcgct ctaggtcacg ctgccgttta cgtgttttat aatcgataaa cagactgtct
    16441 aaatcgtaac ctgtcgggta cagatcttct gctgaacgca ccagacgaac acgctgacga
    16501 ggcacgtgga ttattttttt gtctaaatat acatcaatcg tatcttcacc tatatcttca
    16561 taaactaacg cttctttttg gtattcattg acaaatatcc gatcaccttt tgcaaagagc
    16621 tgacgttctt ttttattctc cttcctattt gtaactgctg tcttaggaag aaattctttt
    16681 ttctgtgtcc gataggcttt ttccgccaag taacgctctg cttgatggat caatttcatc
    16741 gacatatcca ttttatgagc aatccacaaa gcttggcttt ctcctgtttc ttccaacccg
    16801 caactgatat ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc
    16861 ttcatgatct ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc
    16921 cccttgctca tacatagatt ccatgattgc gatcgctagt gcagcccctt catttggctc
    16981 tgttccactg ccaatctcat ctaaaagtac caacgtattc ctttttatct ttttcaaaat
    17041 agcagcgatg ttttgcatat gaccagaaaa tgtactcaac gcattttcta aattttggtg
    17101 gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt cttttgccgg
    17161 aacttgcata ccgaacatcg ccatcaaagt caataaaccg actgtcttca acacgactgt
    17221 tttgcctccg gcatttgctc cggtgataat caagcctcga tagtctttac cgatttcaac
    17281 atccaatggt accgcatgct caggtaaaaa tggatgtctg ccttgcttga taatgatatg
    17341 ttccgattta ttgattctag gtgtgcttcc attgatttct cggctaaatt ttgcccgagc
    17401 aaagatgata tctaacaccg tgatcgtttc gatcaataaa tcgatcactt cttcgttttc
    17461 tgcgatagca ccagtcaatg ccgccaggac ttgatattct tcactgattt cttcagcttt
    17521 taacaactga tagtgttcat tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt
    17581 tcttttattt gattcatcga taatcgttcc atctatttta tttttatagc tggctttgat
    17641 aggaatcgta tagtattcgc ctttttgaac aatcattgct tcttggatca tttctttgtt
    17701 tttcggatga cgcaagaatt tcagtaattt tgactggatt tctttttcga ttagttgtaa
    17761 ttgtttgcgt actttacgca aattacgtga cgcgtcgtca ctgactttat tattttttat
    17821 tttctggtca atcaactctt ctacatttat caaatcagga agatgtttgc tataagcaaa
    17881 caacaagggt gtctggtatt gattcttatc aaaaaacttt gtgatcatcc gactgcttct
    17941 aagaaaatcc gcatattcga tcaaatcggt tggctgcaga atcaaccctt ttttgacttg
    18001 ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga acatgctgac tgctttctaa
    18061 gatcaagcgt gcttctcttg tttcttcttg acgatccaag acagtttgta ggtttgtcga
    18121 gaccgtcata tcgctgattc gttttttgct atagtttcca attgctcttg cttggatttc
    18181 ctttttgatc cgatcaaatt gtaattgact aatcatttcg ttgtccatat tctctccttt
    18241 tcttgaagag atacctgtcc caaaataaat aagggcctag agataaataa aaggatagaa
    18301 cacagcttaa aaaagtcatg tcctatcctt attagaatac aaagatattg ttttcctgac
    18361 aggtaccgtt tcataagcac accaaataaa ctttgtttat tggctattcg acttactgat
    18421 tagataccag ctacactcac aaaacatacc ccattcttct aggtcttatt tattttccac
    18481 ttaactatag caagacacat tttggttgtc aaactttagt atgttttata gatttacacc
    18541 attaatgaat agacacctac cgcgataatt gctccgatca ttggacctgc gattggtacc
    18601 catgaatatg cccaatctga agttccttta ttagctattg gcaataccgc atgtgctaat
    18661 cgaggaccaa agtcccgagc agggttgatt gcatatcctg ttggccctcc taatgaaaga
    18721 ccgatggcta agatcaatcc acctacagcg aatacgttcg ttccaggtgc aaattcgttt
    18781 tgtccaaaag ctagcaaccc aagaactaat acaaatgtac caattaattc tgtgatcaca
    18841 tttgctggat aattccgtat agcaggacct gttgcaaacg ttcctaaaat cgcgctttca
    18901 tctttcgttg cattccaatg tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa
    18961 gctcctaaaa cttgcgcaat gatgaaagga acgacaagat tccaactaat tgcccctgtc
    19021 atcgccatag ctagagagac tgcaggattc aaatgagctg ggcccatgaa tccagaaata
    19081 tagacagcca ttgttacagc gagtccccaa ccaaatgcga tgacaatcca gccagaagcc
    19141 tgtgctttac ttttgtttaa attcacagcc gcacacacac catctccaag caaaactagg
    19201 atcagtgtcc caataaactc ccccatgatt tgagtcatat cactgttcat ttatttctcc
    19261 ccctttagtt cccgcagatc tgattctgca atcaatgctt ctaaacgttt agtctgttgc
    19321 gccttctcct cttctgtcca ttcaaaatat ccagcaattg catcgatcac aggttgcttg
    19381 atctcatcaa gctgatctcg ttcaaacaac agatgatttg tacgacgaat gagataatcg
    19441 cctggggcca aaaccatttc ttcttctaaa ccataacgta accgagctga ctcagcgaga
    19501 ctcaaaccag gataaggtgc catttctttt gctaattcaa agatacgtcg ggcattagtc
    19561 ccgtaaaaat cagcgatata agtagcatct tcttcctcta aaccggctgc tactccttct
    19621 tttgctaatt ctgtcactgt ttcttctaat ttagttggat cgaagtttcc acctgaaatc
    19681 tgatattttt tagagtcgat ttctttcgtc tctattccgt attcttcttt taataattgg
    19741 cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat cggtgatttt cccaccagat
    19801 aaagtcagca aaccatctgg ttctctttct aaagaactac ctctggaaat cgtagaaggt
    19861 gctttttcat cacggctgtt ttccaaatgg ttcaacacgt cttctacttc agcacgagaa
    19921 actttgtttt ccttatattc acttactgta tcgacaactt tattgaagct cttgtctgaa
    19981 atcgatccat tatctccacc attgtaatca gaaccagaat taccaatcaa cagtggacga
    20041 agtcctgccc agcttgcttc gatatcattc aatgtgatat ttgcttctgg atagcgatga
    20101 ttgatcacat ccaatagata atctacatct tcttgtgtga ctttcggatc cgtaaaatct
    20161 ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa
    20221 accatccgcc catcttgctt tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt
    20281 ttcgcatcta caactaaatg gatcccttta gttggacgca ttttaggaga gactggacgc
    20341 gtaaaattca agtttcgtac tttatctacc caaggaccac tcgtattgat cactaatttt
    20401 gctttgattt cgatcacttc atctgtcagc aggtcacgag ctttgacgcc aacgatttga
    20461 tcgccatcat ataaaaagcc aaccgctttc attttactta ctagataagc cccatcttcg
    20521 gcagcctttt tgatattatc aatcactaaa cgggcatcat tgttgcggaa atctagatag
    20581 acacccgcgc cttttagccc ttcttttttc aaaaacggtt ctctttccaa tacttcttct
    20641 ggtgtcagcg tataattctc atatttggtt cctgtcacat ttgccagttt gtcataaagg
    20701 tccattgcta ctttgaccga gaacatattg aaggttgttg ctccttcgcc ctcatagatt
    20761 ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa tactttgtac gactgcacgt
    20821 tcaccaactg tgtcagctac tacttccaca tcaaatgtct tcagataacg aataccgcca
    20881 tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat
    20941 cctgttttga ttcctgatgc tgctgactgg actgccacac cggcaccagt gattccgcca
    21001 ccgatgatca acagatccaa ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc
    21061 ttgtttgaaa acatatggat ttcctcctta ttcaaatgct tgggtagctt ttactgcttt
    21121 tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt caaatagctt
    21181 gccagcttca taaaattcgc ggatctcgtc tgtatctttc caataaccga cagctaatcc
    21241 tgctaaaaat gctgccccta gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac
    21301 attcaagata tctgtttgga attgcatcag atattcattg tttgctgcac cgccatctac
    21361 tttcaataca ggaatcttga ttccagtgtc atcttgcatc gtatcgatga tatctctcac
    21421 ttgataagca atcgactgta aagtagcttt aatgatatct tcttttgtcg taccacgtgt
    21481 caagccaaac atagcccctc gtgctgcctg atcccaataa ggagcaccta gcccaacgaa
    21541 tgcaggaaca acataaacct catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc
    21601 gctagctttt tgcaacattt gaagtccatc tctcagccat tgaatcgaag aaccagccac
    21661 aaaaatactt ccttctaaag catagtaaac ctttccattt atgccgtaac caattgtcgt
    21721 caataaatta tttttggata attgcggttt ttcaccggta ttcatgacaa taaatgaacc
    21781 tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc
    21841 ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat gataaccttg
    21901 cgtataacca taaacttctg agttagaagt gacttttggt aacatcactc gaggaatatt
    21961 tagtaaatct aaaatctcct gatcccaatc tagctgatgg atattgaaca acatcgtccg
    22021 actggcattc gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc atactaacca
    22081 agaatcgatc gttccaaaca tcaattcgcc tttttcagct cgttcttgcg cgccttctac
    22141 atgatcaaga atccagcgta tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt
    22201 tttttcgtgg aaaaattctt gatagccttc attttttaag ttatctgcaa ttccagctga
    22261 ctgtctggat tgccaaacaa ctgcatgata gattggacgt cctgtatctt tttcccatat
    22321 tactgttgtt tcacgctggt tcgttatgcc aatcccagca atctgatttg gtttgatccc
    22381 tgattcgatg aacgctccgg caataactga ctgtacagaa ttccagattt catttgcatc
    22441 atgttccacc caactttctt gcgggaaata ttgcgtgaac tctttttgag aactgccgat
    22501 gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta gttgtacctt ggtcaatcgc
    22561 catgatatac gttgattctg tcattgtgat tccctccatt atatgcaaac gctttcttta
    22621 actactctta tcatactact gttttaaaaa aagaaactgt caaaatttta ttcttttttt
    22681 ctttttttga cagtttttta ttatgaagct attttttaat caattctttt cttttttcag
    22741 cgtctctatt cttcttttca atcgaataat gtcgtaaggt gacgcaaatt ctgacagata
    22801 atattcacac tgttttttgt ttctctggca aaatgtgatc accaaatcat attctttacc
    22861 ttcttcataa cttccaatct caataccgcc gattcccctc agttctctga tcagatattg
    22921 ttgaaaagca atacgataaa tcggcagact ttctaaatcg attccaatca ctactggttt
    22981 tgcaatataa aaatcgatca tcaaaagaac attggcatac tggatcatca ggtaactaag
    23041 ctcactatct tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc
    23101 cagtaactgt gttaaggaat gcccgagcat tttctgctgc tgttccaaaa gccgatcacg
    23161 ctcatagact tcgatctttc caacaaagaa aaaccattca ttgttgatct gggaaatttg
    23221 gtagcctact gccttttctt ctttgatttt caaacgacga ggacgataat gccataacat
    23281 cgtttcccga atgtaagtat ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc
    23341 atactgataa aatatttctt cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat
    23401 cattgtttcg tatacattca gttctgctgc tgttcgacta agatgcatca cgatgattgg
    23461 atcgattgct tgataaagcc ggtccgactg atagagtaat ttcttttctt tcaaagttgc
    23521 gtacgtactt ttttcattca atagtctttt tttcgtaatc ccgagccagc aagcaatctg
    23581 tgcttctgct gatgccgtaa acgttgtttc taatacacgg tccaatccta gaatgaacgg
    23641 tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga tatattggaa
    23701 tagttcataa taaaaatatc gtatctgtag ctcttctccg tataattgac cgtttttgat
    23761 ttgtaatcca aattcctcta agagctggtt caattctttg atctttcgaa aaacagaaga
    23821 ctcactgata ttaaacgctg tcgctaatcg aacgatggat acttgctgat gttctaataa
    23881 atagacgagc atcttaaact tcaagctttc ctgcaataaa aacaatagaa ttttttccaa
    23941 acttacatct tcgtccaaca caagcagcgc ctgatactca tttcgctgta cttctacttt
    24001 tttttccatc attcggccaa tcaattcaat atcttccaaa tattgatcaa acgaggcttt
    24061 tgacagttgg acatgctctc tcaaaccatt gatagaggct gttccccctt gcagaacaag
    24121 gtttcgcaaa atcaggatct gtaactgctc ttttctttct aataatgctt caatcttcat
    24181 aggcagtatt cgccaccttt gaccacacca tatctggcac acggtattct ggctggaaaa
    24241 atagctcatg ttcttctaac tgttcaaacg tccatccttc ttctactgca attgtcagtt
    24301 ggtcaagaag gtggaacatc tcttttttgc tttggagctg tgctccaaga atcttttttt
    24361 cagaatcaaa tagaaatttg agtttaactt tgctttgatt tattgctgta agcttgtagt
    24421 ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct ctcttcttct gtcaaaccta
    24481 cgcttcctaa gaaatagcca aaccactgat tgccgactgg acgataggtt ccctgatcct
    24541 ttgtctgtga gccactaatt gtcttgctga catttcgtgc tgtacggaca gcgttattta
    24601 ctagagaaac atacattttt tcatttgttg gacgaaaagt tacttggatc gcatcgccaa
    24661 tcgcataaac gtctttttga gacgtttgca agtagtcatc tacccagatc gttccatcat
    24721 cattcaatgt taattgttct tcccacatct gattgtcggg acgagaatga ttagccagca
    24781 aaacaagatc atagttctcc tttctttttt ccgtctctac aatagcggtt tcattttcgg
    24841 tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc atttccttta
    24901 ctaacggttc tgtcatttca ggatctagat aacgaaaaag aattgacttt cggctttcgt
    24961 aaagatggat ttgttttttt cttgatgcca acccttcagc tatttcgatt cctgcttgtc
    25021 ctgcaccaat aattgcaatt ttctttgcat tcatgatctt ttgctgaaga gccgttacgt
    25081 tttgtatctt tttaactggg tgaataagcg ttgattctgc agaaatattt cgaaactttt
    25141 gatttgaacc agtcgctaga atcaaacgat caaaatccaa tttttttcct cctgctaaaa
    25201 cgactttttt ttctttcaat cctattactg tcttttctgt gtaaacatgg attcgatagc
    25261 ggtcagtcag ttcttctttc gtgatccaag aaagttgttt ttcactggta acctttcctt
    25321 ttaatatcaa attgaacccg ctgggaatcc atcctaactt cgattgcttt tcaatcaaat
    25381 atatttcact tgttggcatc aattggcgaa gataaatagc cgcatgaatc cctgcaaacg
    25441 acccgccgac aattacaatg ttcatctgca ttcctctttc ttataaggtg ttctactgct
    25501 aacttctcaa tcagaaaaca cagtattcat aactcgcttt caaccttatg atacatagcg
    25561 atgcagcaga atcaaccctc tccatcatac cgtgttctag gtctgcttct caataatgta
    25621 ctagtggacc taattgaaga gtatcatttc tgaagagatg tttcaaaaaa tgtactctta
    25681 taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa taggaaacag aaaagaacga
    25741 aaagacgagt tttagcgtgt gtaatgtaat agttagaata gacacatcat ttatttttaa
    25801 ttctttaatg cttcaacaac tgcttcgcct aattgatgag cagattgagg attttgccct
    25861 gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag ctgctccaga ctgatagttt
    25921 gctccttttt ctttcaaagc atcttctgct aagaatggta cttcattcgt tgtaccattt
    25981 gcttcctctt cttcattact gaacccagtc actgtacgtc cagctaaaat agattttcca
    26041 gactcatctg taactggcaa caatcctaca acaccgtgac aaacagctga gaccagccct
    26101 tcatttcgat agatttgtga agcgactttc ccaagatcta ttgcttctgg aaaatcccac
    26161 attgttccgt ggccaccagc ataataaacc gctgcataat catttgcatc cacatcatca
    26221 ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt catcttcata ataggcccaa
    26281 tcgatttcat ccatattcaa aacactttgc ggttctaaag gtacatatcc gcctttagga
    26341 ctgacaaaat ctgcatcgat cccatttttt actaatacat cataaaaatg agtcagttca
    26401 cttaaccaca aacctgtagg acgctcgatc ttttcgtact tttctacatt tgttaatacg
    26461 atcaatactt tcattttccc atcctctttc tataaaaatt gcgttattga taacgtgctt
    26521 tcaagaaatt atataatggg cttggcaaat aaagctcttt atatgctttt tcaaaaggtt
    26581 tcgttacaaa ttcatcctca cggttttcca ataatttctc tgcccagtca ggatcgacga
    26641 tcatttgctg tccaattgct gcaagttctg catatttcaa tacctgctct acatcttcgc
    26701 gattgcggat cccaccgaca ccaactaaag ggatctttcc ttgaatcgtg ttatggacca
    26761 cttcaagaat cgatttatcg ttgtatttat cagaacgagc tattcgatca tacatattta
    26821 atgaaacatg cagataatct agttttgact cacgtaatcg ttcaagaagc cagatcgtgt
    26881 cttcaaaacg gatacctggt gtttcatatt cttctggtga gaaacgatac cctacgatga
    26941 atggtttttt agcatattgt tcaaccgctg agaaaaccgt tgttaataat tcttcaataa
    27001 aacgataacg attttccaag ctaccgcccc attgatcctc tcgtctattc gaatgcggag
    27061 agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg
    27121 attcaatcgc tctacgtgtg gctttgccaa atgcttggat tgtttcaacg atttcttctt
    27181 cactcattgc tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca cttgcagata
    27241 caggctgttc tccagataaa gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa
    27301 taatcgcttt tgcgccttct ccttggattg ctttcgctag ctcacttaat cgaggaatca
    27361 tttcatcatg agcgatgctg agttcacctg gccagccttt tccgatatct tggacatttg
    27421 cagcaccagt aataacagca ccaagacctt tagaacgttg tttataataa gtgatctcat
    27481 cttcggtgat cactccattg aaaaagctga gttgtgtcgt cataggcgac agcatcaatc
    27541 ggttcttcaa ttgaactcct gaaggtaaag taattgattg atcaaattgt gacattgtca
    27601 ttcctcccat aattcgctct gattaaccta tcgagaatca tctatcatta aaccactcga
    27661 ttagttgtac atgcaactaa tttgcttaaa aaattttatt ttttttgtaa cttatctgtt
    27721 gcatcattca ttgtctgggc taattcttta cttgcttcat ttcccaaaac tgctttgtat
    27781 gactgatgaa gttcttccca gctaccatcg acttccttag caaattggcg tccttgttct
    27841 gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc tttatattct
    27901 aatttttcaa caaaacgagc aatcgttgat ggggcggaat agagcatttg tgcaatatct
    27961 ttttgtggta ttccatcttt ttcttttaat atcaataaaa taaaaccata agttggtgcc
    28021 agacctgtcc ttctaaacgc ttcatcagct aatttattca acatgcgatc tagctttttc
    28081 acgctaaaga aaagacattc atctaacgct ttttcaattg gcttttccat ttcatcccct
    28141 ccattggcgt acatatcata gtatgatttt tattttttgt caaaaaacac ggaataatgt
    28201 ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg
    28261 ttttcgacaa aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt
    28321 tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
    28381 taataccgaa aaactggctt ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc
    28441 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttgt cgttttttga
    28501 gctaatgccg atggtcagct gattgtacac cgtttattga gaggtcgtga gcctgccgtt
    28561 gaactccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttta tgactatggt
    28621 gacttgtgat cgaggagttg gcaggagaat actgtttatt cgtaaagaag gggctgtgac
    28681 aagacttttg tcacagtccc ttatttcctt ttattatagg atttcgccat ttgattggat
    28741 gacttgtctg taccaatcaa atgatttttt ctttaaccgt ttctttgtgc cattcccttc
    28801 gtcatcttga tcaacataga tatatccata acgttttgac atttgtgaag tagaagcgct
    28861 gacgatatca atgcagcccc acgacgtata accaaataaa tctactccat ctttgatagc
    28921 ttccttcatc tgctcaatat gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt
    28981 tccatcttct tctactttgt ctatagctcc gagtccgttt tcagctatga acagtggttt
    29041 ttgataccta tcatataatt gattcaaagc aattctcaaa cccttaggat caatctgcca
    29101 gccccattca cttgatggaa gatacggatt tttaattcct ccagttatta agtttccttg
    29161 gaccccttcc caattttctg gatgagcact gactgcacta gacatgtagt agctaaaaga
    29221 aacaaaatca acagggtagg ctgctaagat ttcttcatca cctacttcta tttttaagtt
    29281 gacacctttt tcttcaaaca ttcgttgcgt atacgctgga taatatcctc ttgcttgaac
    29341 atcactaaag aacaaagaag ttctttgctg ctcaattgtt ttttgaatat catccggatc
    29401 acaagaatac ggaacaagaa gctgatagct gatcatacaa ccgacttgtg aacctttgat
    29461 aatttcatga caagcttttg tagctaaagc acttgctata aactgatggt gtgcaccttg
    29521 gtatttcgct tgttccaaat ggggatggtt ttcttcaatt actcctacgc taacaaaagg
    29581 gtgatgtttt acacagttga tctcattgaa agtcatccaa tattttactt tatttttata
    29641 gcgtttgaat actgtttcag cgaaacgaac gtaaaactga ataagctttc gattaggcca
    29701 tccaccatat tctgtaacca aatataacgg catttcataa tgggaaagag aaacgactgg
    29761 ctcaatccca tattttagac attcgtcaaa cacttgatca taaaaaaata atcccgcttc
    29821 attgggtgtt tcttcatcac catgaggaaa aattcttgtc catgcgatag acatccgata
    29881 gcatttaaaa cccatttcag caaataaacg gatgtcttct ttaaagtgat gatagaaatc
    29941 atttccgtgt cttttaggat aataatactt gctgttggga tcctgcgccg cttttacttg
    30001 ctgatgcgtc atcatatgcc attgatctgt ccatttttct ttaggtaaat cgctgtcgaa
    30061 tgtatacaca tcagacacag ataatccttt ttcatctaca tcccatgcac cttcacattg
    30121 attggccgct gtcgctccgc cccataaaaa tccttctgga aattttttca tatcgatccc
    30181 cctaaacttg ctagttttct tgtttgatta tttttcttat gactagtaat gatatttttg
    30241 aatctactac tatttgtaac aataaccatt gtggtcggat catatccctc tttgattaat
    30301 tcttgcaaat ctacttccat caataaatcg cctttacgta ctttttttcc atcagatact
    30361 gcactttgaa agtatttgcc atttaaattc actgtatcaa taccgatatg gatcagtatc
    30421 tctgctcctt cttcactgac taccccaatt gcatgtttcg ttggaaaaac tgttgaaact
    30481 tccccgttta cagaactgta gatttttccg tccgttggaa tgatcgcaaa cccttctcct
    30541 aaagatccac tagcaaatgt tggatcgttt acttcagata aatcaattat ttcacctgct
    30601 gcaggagtac ttaacactat atcctttgag gattcttctt cgatttcatc cttgtagccg
    30661 aaaagaaaag ttagcataca ggaactgatc atcgctaaga aataactagc aactagccaa
    30721 acaaatcctt caccaatata aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa
    30781 gcaccggctc ctgcggcacc agctactcca ccaaatatag ctccacatat acaagcaatg
    30841 taaaatggtc gtttatattt aagagtcaca ccataaacag caggttcagt gattccaaat
    30901 aatgctgtga taaatgcaga taaagataaa ctttttactt tcttattttt cgttttcaaa
    30961 aataccccaa gtactgctcc tgcttgcgca gctacggcag tcatacatgc tggacctagc
    31021 gtatcacgac cgtagaaaga aagattgttg gtcattactg gtacaagtcc ccaatgtaac
    31081 ccaaaaatca ctaacacttg ccaaaatccg ccgataacag caccagataa aacagggcta
    31141 aagttataaa cagctaaata tccagctgca atcagctgac ttgcataatc tgcaattgga
    31201 cctacaacta ataaaataat tgggaccatc acgatgatac aaatgaatgg tgtcaataaa
    31261 tttcgaatac tttcgtgcaa gcttttgcga atcttaggct caatataaga aagaacccat
    31321 acagctaaaa ttgctggaat aaccgtactt gtataccttg ctagaatcac gggcatccct
    31381 agaaatcgca aggttatgct atcactatat gcactgacaa tcgtaggata aacgagagct
    31441 gctgcaataa ccatagcgat gaaacgatct gtattgaact tttttgcagc agtatatgcc
    31501 aaaaatatag gcaagaaata gaaaaatgca tcagctgctg cattcaaaat gatataagtt
    31561 cctgattcag tagttaataa attcaaactc gtacataagg cgagtactcc tttgatcaaa
    31621 ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat gtctataaaa
    31681 gagtttaaaa tgcctttttt ctcagttcct ctctttccac tagatgcatc atcttctagt
    31741 ttcatttgtt tggataatac gtcatacact tcatttactg catttcctac tacgacttga
    31801 aacattcctc cagcttcgac tacagtgatc acaccttcta gttgctctac cttctcctta
    31861 tctgccttct tattatcttt tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg
    31921 ctgacatttt cttcgcctcc gacaaatcgg acaatatctt tcgctaattg tgaatagtcc
    31981 atttttactt cccccttgtt aattaatgcc gtagcaatcc ttcttacaag ataagtatag
    32041 ttcttagaaa acggtttctc cattcaatta gtaccccttc taatagcgaa cgaaactgta
    32101 caatagtgtg tatggtttac ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt
    32161 atatcgtttc tcttatagtc aaaggagaca aaaaaaacac gcttgatctt cctttgctga
    32221 tgtataagtg gtatctcaag ctgatactct gtgatttttg tttctacttg gcttttgata
    32281 agacagcaca acaaagtctc ggcatttaac ggaagcataa ctaggtcaga actaggagag
    32341 attagttgct gaatggaatc tatcttttta tggattttta tttgattttc ttctttgtag
    32401 taatgatcaa gcagttttgt tttctttttt tcttcaacta tggtcagctt ttcacgcaaa
    32461 atctgataaa tctcccaaga ttcaaactca tctagaacac taggtagata aatcaaatca
    32521 tcccactcaa aaataagctc ttgctcagta gtcaaaattg catcatattc cgttattttg
    32581 tttgattgat ttttgaaaag atacactggt tctatccggc attctttgat ccacatgttc
    32641 atactttgtt gaatctgttt ttttagtgta ttcaaaatac taattggctg gttactaaca
    32701 atcagtatct ccccttgttt tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa
    32761 taagcgcttt cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt
    32821 aaataaattt ctaaaggata acgggagatg atcttttgta caaaatgatt catggtatta
    32881 tgtccggcat ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca
    32941 tggaacaata acgaaacagg aagatctaaa aaacgaataa tcgtttgctc aaatgctctt
    33001 agattcttct ctatttcttt gtttttttca tcattaatca tcaaataatt taactctaat
    33061 agtcgatttt caattgcttg tttttcttga ttggaaaaag aatagggtag ttctgatgat
    33121 aacttttttg tgagggtctc taccatcgga ataaataatg tatacctgtt tgttttttct
    33181 aaaagttctt ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc
    33241 tctcctgaaa caatataatt atatgatata agaacctctt ttaatgtctt ttggacagtc
    33301 ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa accttgcgtg ttggatcacc
    33361 gtttctgttc caacaagaga taagaaaatt ctttttgaat tttcttcagc aatgattttt
    33421 agtccttttg tcgacgaaac ttccagttct aatttaggat tgcgctcgat ccaacgcaaa
    33481 atcgttttta tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt
    33541 tgcatactga catacccttt ttgaaacaat aagtacgtca caatagttga agctcggaaa
    33601 tccatgctgg aatagatttt atgtttcgtc acctcttgta tgatttgctg tttagtttca
    33661 gattcaattg attctaaaaa ataacctgaa cgtttatttg actggataat gattttcttc
    33721 tcgtagatag caagcatatt tttgatttca gtttggatcg ttttcttgtt ggtacctaaa
    33781 gcttcagcta attcttcgct tgttatccaa tgattcttat ttcttaatac tacaagaatt
    33841 tggagttgtc gtttatttaa gtccatttgc ttacacctcc tctttgttat aaacatacag
    33901 gaaaaacctc tcaaatgaca atagtcactt gcagaggttt aaaaatcatt tgaagaaaga
    33961 tctatattca aggtttttac cattagactg gataatttgt ttataatatt caaaactatc
    34021 ttttttcaat ctcctaaagc ttccgctccc atcattttgt ctatccacat aaataaagcc
    34081 atatcgcttc ctcatttccc ctgttcctgc cgatacgata tcgattggtc cccaccataa
    34141 gtaaccaatg atttggcaac catcttttat tgcctcagca atttcctgaa gatgtatttt
    34201 tagatactca caacgttttg gatcgtggat tcgattcaat tcatctggtg cttcatctag
    34261 tccaatgcca ttttcaacta tgaataatgg taattcataa cggtctgtca attcattcat
    34321 gacataacga aaccctagag gatcaattgg ccaactccaa ggttcgggag atttttcctt
    34381 caaaaaggga ttatcgaaac catttgctcc gccagtatct acgcgcattt ctgcttcttt
    34441 tttatagaca gcgctacgat aataactaaa tgcaatataa tctgccgtgt tagcttgcaa
    34501 gcactgttcg tctccatcta acattactgg tgcacagtct tcttccttcc atgtcctgta
    34561 aatataagct ggataatgcc ctcggcacat gacatcggag aaaaaccatg aattgcgttt
    34621 aaattgcagt gaaccaaata cattctctgg atcacagtca tatgcataag tcgctaatgc
    34681 agaaaagcta cacataatac ccatcttagc atttggatcg attttcttta ataattgagt
    34741 agcacgggcg ttcgctagaa acatatgatg acaggcttga taaatcactt tttttgttag
    34801 tgtagcaatg ggttcaagtt tatctctagc agggaatgta accaatccag cagctgcata
    34861 aggcatacga aaagcattat tgatctcatt aaaggtcatc caatatttca ctttcccttg
    34921 gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg
    34981 ccacccccca tactctccta ccaatgctaa gggggtttca taatgactaa gagtaattac
    35041 tggctcgata tcatatcgat ttaatgtatt gattagtctg tcataaaata cgagaccagc
    35101 ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg
    35161 aaaagctttt aaacctaact ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa
    35221 atcaatcgct tcatgagaaa gataatggag tgattcgtct aaagcaatct cccaaatctt
    35281 tttttcttca ttccactgaa tactaggatg ctggttcatg atcccgataa gcgtatctgt
    35341 cacattaggt aatttgccgt cttccaacca tgcaccttca cattgattag cagctactgc
    35401 tccgccccat aaaaaatttt ctggaaatat ttcctgatcc ataactatct ctcctcgcta
    35461 tcaacaattt cttacttgaa gtatagctcg attcatcaaa acaagatatt ctaactattt
    35521 cggcagaaga gaagaaaaaa attgaatgct actcttcatt tttaatattt atatataaca
    35581 aatacataag acgatccccg aaagaaaaca ctcgttaacc atctaagcaa acgatttatt
    35641 ttacttccaa cacaaatgct tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt
    35701 tcagaggata atttgacaca ataaccctta aagcttcacg tggaatagtg aacgtctgct
    35761 tttcactgga catattacaa ataacgagcc attgctggtc ttcatatatg cgagaatagg
    35821 caaagacatg ctcaggcgtc ttttccacta attcatagtc tccccagacg atgatgtcat
    35881 gttttctacg caaatcgatc aatttcttgt acatataaaa aacagaatct tgatctgcta
    35941 acgcttcttc tgcattgatt tctttataat ttggattgac tcgaagccat ggaattccag
    36001 tagtaaaccc agcatgttca cgggcgttcc attgcatagg cgtcctagca ttgtctcttc
    36061 ctttcgcctt gattgattcc aagatttcct cttttgtaaa tccagaagaa atcctttcgt
    36121 tatacatatt gattgtctca atatcttttg cttccgcgat atcatcgatt gggcaattcg
    36181 tcatgcctaa ctcttctcct tgatagatat aaggggtgcc tttcatcata tggagcaaga
    36241 tagcaaacat cttggcactt tcttttcgat acacatcgtc atttccccaa cgagaaacaa
    36301 ttcgcggaag atcgtgatta ttccaaaata aactattcca tccttcatca cctaaagatg
    36361 tttgccattt ggataatact tttttcagtt cagagatact caagggtttc aaatcccatt
    36421 tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa ttggaagacc atggataatt
    36481 catttctttt aggattggaa taaagtttag cgatttcggt agttgctccc catgtttcgc
    36541 caacagtcat cagatttttg tcaccaaaag ttgcttgatt catttcctga agatactcat
    36601 gcagtttagg tccattactt gtgatttctt tatccggttc tttgccaatc atatcgatca
    36661 cgtccatacg aaagccccca atacctttat cgatccagaa attcatcatt tgataaatct
    36721 cttggcgaac ctgtttattt ttccaattca aatcaggttg acgcttgcta tacaagtgca
    36781 gaaaatattg ccccgttttt tcatccaact gccaagcaga accactgaat attgagcgca
    36841 atctattagg ctcttttcca ttgaccggat cacgccaaat ataatagtcc cgataaggat
    36901 tttctttatt tttacgggcc tcaatgaacc acggatgttc atcagaagta tgattcacca
    36961 ccagatccat gatgatacgg atatttcttt tctttgcttc ttcaatcagt tcttccattt
    37021 cttccatcgt accgaattct gccatgattg cttgataatt actgatatca tacccattgt
    37081 catcattcgg agactcgtat actggagaaa gccaaattgc atcaatacct aaatacgcca
    37141 gataatccaa cctggaaata atcccccgca aatctccaat accatcttga tttgaatctt
    37201 gaaaacttct tggatagatt tgataaacaa ctgaatggtg ccaccatttt tgttccatga
    37261 ttttcctcct ataaatggat gtttctattt tttgcttaat catttatttt agtctctctc
    37321 actaaaaatt gtaacgcttt caaatagcta ttgcaccttg ttatcggtaa cagaataaaa
    37381 caaaaaaaca gctgaggaaa ttccctcaac tgttttttga aaggattgat ttatgcaggg
    37441 aagaatgctg atttcacaac ttctttataa acggtgttca ctcagctgtc ccttggtatt
    37501 agctcaaaaa acggcaaaat ataagaaact attttttcgt ttttttctct aatactcagg
    37561 ccagaacgct gatttcacaa cctctttatt tcacattcaa tatccacatg aatcccacaa
    37621 atacaaagaa cagaccatac atgattggag atacttcttt tcctctttta gctgctaaca
    37681 tggtaattgg atagaagata aaacctaatg caatcccatc tgaaatacta taagtcaaag
    37741 gcatgccgat caagatcaag aatgaaggga tcgcaatctc taagtttccc cattcgattt
    37801 gtcgcaaaga ttgtgccatc aatactccta ccataatcaa tgctggtgca gttacttgtg
    37861 aagtaacaac tgatagtaat ggagaaaaga acaagcctaa caagaagaaa atccctgtag
    37921 taatagctgt gatacctgaa cgtcctccaa cggcgatacc agctgatgat tctacaaatg
    37981 caccaacagg agaagtacca aataaggaac ccgcaagcat agctgttgaa tcagaagcta
    38041 aagctttccc tacacgaggc atcttgttgt ctttcatgaa acctgcttgg tttgctaatc
    38101 caactaaagt tcctgctgta tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc
    38161 acatttgaag agaattgatg tcccctacat gttttaatgc aactaaaaat gttggtttca
    38221 aacttggtgc tgcggcaaca atttcagaag gcattggaat caaacctgtg accagaccta
    38281 aaatcgttgt agctgccatc ccgatgaaga tgccacctgg tacacgccga acaagcaaga
    38341 tagcagttac gactaatcca aaaatcgtca accaagtaga accaacgttg agtgatccta
    38401 atcctactaa tgtagattca ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag
    38461 cgataaatag accgataccg ccagaaatcg catatttcaa atctgctggg atcgcatcaa
    38521 tgatcatttc ccgcagcttg aagattgtga tcaaaataaa aatcaaagaa gcaacgaata
    38581 cgccggctaa tgctgtttcc catggaatcc ccattcctaa acaaacagaa taagcaaaaa
    38641 aagcattgat cccaagagca ggtgccgttc caattggata acgggctaat acccccatta
    38701 aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa cactgctcct tcatccattc
    38761 cagaagcacc taatacagtt ggatttacaa ataagatata agccattgag ataaatgttg
    38821 taaatcctgc cagtacctca cgttttaccg tggtgttcaa tttttctatt tcgaagtagg
    38881 aaagtatttt ttccttcatc ttccatcccc ctaaatccga acattataaa taaagtccgt
    38941 taaaatttcg atttattaat aaaacaacgt gttttatcat atactaaaac aaaacataag
    39001 taaacaaaaa attcggattt taagcaaaat ccgaacgttc catctgatta aatcccttta
    39061 tctcaaagtt catgaacatt cactttggat tacaagaatt tctttattgt gttaataaag
    39121 tttcttcatt ctagtctcaa aaatcaatag tgtgatttga ttttaccatt tcgtttctgt
    39181 tcttctttga tgaattcttt tgtatcgaca ggattcagat tatttagtcc gccaaaacct
    39241 gtatcgacca tttttcccca gccttttgtc ttcttttttc ctgatttgag attctttgct
    39301 cgaagcttct cactatgttt tgctcgccga tacttcaaat aaccttctaa tcttcccatt
    39361 agatccactg tttttcctcc tttttattca atcagacgac tcagcttttc ctataacaaa
    39421 ataattgcct tcagggtcag aaaaattgaa agtcaagatg ccattgttat ctgtgatttc
    39481 tcctggttct tccatgttct cgtagatagt ttcaaattca tccgtaaaaa atactaaaga
    39541 aggcggtggt cctaatacct ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc
    39601 taattctaat tcttttgaga tgctgagtac aatactctta aattcttctg gcaattcgat
    39661 tgtttcagaa atttctgcac caattttttc tacaaagaat cggcaaatca tctctacgtc
    39721 attgacataa agcatcacac gtgttttttg tatcatcatt tgacccttct ttctatttga
    39781 ttttctttcc tgtctataaa ctgattccat tatatcacga aagcgcaaac accttgtgtt
    39841 tgatactatt caagaaaaat aaagcaatag tttctaggat tgctttcctc cacaaacttt
    39901 ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc
    39961 tgcttctgat tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc
    40021 gattttttcc ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat
    40081 ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc
    40141 acgccagttg agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg
    40201 aagtgccgga gcatcggaat ctgcacctaa aggcaggaat gattcgccac cttgggcttt
    40261 tcctaaatgc agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa
    40321 agacaaggaa atactattat agatatctac aagcggattg attgggaaaa attcccgttc
    40381 ttgatttgca cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa
    40441 tgtggtaaat gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt
    40501 aaggtaattt cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact
    40561 ttcttttgtt acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg
    40621 aaatacttcc cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat
    40681 tagaataatt gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca
    40741 tgttcgctgt atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt
    40801 tcccaaacac cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta
    40861 tctagtaaaa atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg
    40921 atttctaaaa aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg
    40981 taacccgcag gctcttgatc gatactcact aggaaaaaat cggtttcttt atcttttagc
    41041 tcttctgtaa gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt
    41101 gaattatctt ttccaaatgt atctgtaaat gtttcgatac taatttgttg tagtaagtct
    41161 acatctgaag gcgtcatttt tcgaatttcg tatttattca cggcttcttc ctttcaatag
    41221 atccttttat tgcctttttt gacaaaattc caatcttcgc taacattctt gcatacttta
    41281 tttaaaagat attccaattg ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca
    41341 ttggagtagt tgttttctcg aatgatgaaa ggatagattt ctgctccttt tttagtcgga
    41401 tagagtttct tgattttttt attttcttta tcttccaaac gttcgatcat cccattggat
    41461 tctaatttct tgattgctcg ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt
    41521 ttttcttgga tgattccagg attttcgcaa atacgtacta aataaagata ctgtcctctt
    41581 gttaaatcga cttctttgaa ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt
    41641 ccgatgtctc gaaggatttc agccatttca tcccaccttt attttgtttg taagactagt
    41701 tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa aaatcaagtt cttttttaat
    41761 aactagtaaa gtatattctt ttcttctttt acgaaaaagc tcaatcacaa tagatctggt
    41821 ttttgttttc taaaataagt aactgtttct tcgttgttcg cttttctgat agctttggca
    41881 actcttctat actaaaaaat cccatgttgg aagtttcatt attctcaata aacttggcct
    41941 cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat
    42001 ctttttgttt attcgtatca aaaactgctc gcaactgagt agtttctact gttaatcctg
    42061 tttcttcata gacttcttta cgcacatttt cttcaggaga caatcctatt tcagcaaatc
    42121 caccaggaag tgaccattct ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt
    42181 ttttgatata ggcacgaaca tctacttttg gtgttggatg tccttctcca atcgttgtga
    42241 gcgcttttat ttcttcgaaa gtttcttttc ctatttcact gatcagttcc aatgaaatcg
    42301 tccggatctc ctcaaaacgc tctttatcaa atacatcttt tccataatat agaccagctt
    42361 gagaaagtgc gagtagtctt ttgtattgag ccaacttctt gttcattctc ttcccttctt
    42421 ttttagcaaa tgtatgacta tgtaatcaag tatagaagac tggtttaatt acggcaaata
    42481 aatataatga aatctgcaat aaataaaaga atgttaaaaa actcgagaac aaaagacatt
    42541 cgacttctct ggggctgtga ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc
    42601 ccaccaaacg aaaaatatga cgagctattc ttgtctggct gttcttatta attgaggcag
    42661 gacagctttt tcacaacctc cccactaaac ggtgttctcc taccaactcc tcggtcacaa
    42721 gtcacaatat tcatcaaaca tgagaagctg tttttttgaa attgctcctt gtgactcgga
    42781 gctgaacgac aggctcacga cctctcggta aacagcgttc aaaagccaat ttttcgatat
    42841 taagccattt ctttgttact tattgtcatt gatttctttt tctaatttat ccaatagtgg
    42901 agtgtaatcc acttgagtat agtctgttac atcaaattca tatagtgttc ctaactggaa
    42961 tttgtcatat tttcggtcgg cgatgatctt tttgaatgct tctttcgtaa tatgattggt
    43021 ccctttcgtt cgatcttcca atacgtcacc atagtgataa tgatccatga tatagctcaa
    43081 atgacgatct gattcacgat cacgtttgta ccgacgttgc cataacactt caaagtctgc
    43141 tgttaggcga atcgtgatca cctgatatcc gtatctatct gctaattctt ggagtcgtgc
    43201 tttttgctta tcactaaaag gatactcgga cacaatcact ttctttcctg cttccatata
    43261 caaatcaagt gctttgtaat agaaaggata aactttcgtc ctttcttgtt ccgcccgttc
    43321 ttccaaattg ttaaacccga tggattctgc atagtattcc ttgatttcat cgagcgtgat
    43381 ggcatacata tctggaaatc gttcccgtag tttattcata agatatgttt ttcctgtacc
    43441 aggactacca gccagaagaa tgaaatactt tttcatctca tttatttcct aagttaattg
    43501 gactttccaa gttcataaac agtccaatct tatgcttgac aatttctttt actgcttcat
    43561 ttgctggaat cacattatgg cgaagagact tgttgatttc tgtttcacta aaggtttctt
    43621 tagctacttt cgtccagttg actaaaagct ccgttccaac attgaatttg cggatcccgt
    43681 tattgatcaa ttcaggataa tcttcttctt tcacacctgt tcctccatgg ataactaggg
    43741 ggacatcaac tactgcatcg atttctttca gcaagggaat gtttacttct gtttttgatt
    43801 tgaattgtcc atgattcgta ccgatagcaa tagctaatgc atccacgcct gtagcttcta
    43861 caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc attttcagcg acatggattc
    43921 cttcttctgt accgccgatt gttccaagtt ctccttctac ggaaacaccg tgcgcatggg
    43981 catattctac tactgctttt gtcttcagga tattttcttt gaaaggcaga tgggacccat
    44041 catacatgac agaagtatat ccgctgtcga ttgcttcttt gatatcatca aagtctcggg
    44101 cgtggtctaa gtgcaagact gcatcaacca tttcttcttc tgcaatagct gtcattacat
    44161 tgactaacac atgcatgccg atatatttag cggtatcgac acttgtttga ataatgatcg
    44221 gcgaacccat ttcttttgct gcgcctagca tagcaggaag catttctagg ttatgcgtat
    44281 taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt tgttacttct ttcaatgttg
    44341 tatacatctt ttattgccac cttatctttt tatttgtttc ctttgatcgt tacttgtttt
    44401 gcaatttgtc gcatctcatc cattttgccc atgtagtaat cgatcccttg atcatcacca
    44461 gcacgagcag catctgcacg cttctggtta tagaggctca tcagtttttt atacccatct
    44521 ccgatcgtct gtttattttc caaactcttt tctaaatatt ggatggcttg atcgttcaca
    44581 tacaattcag aaaatttaag tccaacttgt tcataaagtt tcgctaattt atgcttatct
    44641 tgggtagtgg aaatctcttg tttcaattgt gtaatctctg attgaatctt ttcggtcgct
    44701 tctggcgata gggctttttc ttcttttgcc agaacttttt tcggttcttt ttcttttttc
    44761 ttaaaaaatc caaacatcat tttccctact ttcaattaaa atgctttcaa aactgggctt
    44821 aagatccagg catataagag tgctgcggct actgtatcta catcggttgc tgtagcattg
    44881 ttgaatccca tcgaattaaa gatcgttact agcaaagcgg gcaataatgt aataaagaaa
    44941 ccatgagcaa taccgccaat gatcgctcct cggcgtccgc caactgcgtt gccaaatatc
    45001 cctgctgtcc ctccggcaaa gaaattggtt agcataccag gtaaaatcat cgctaagccg
    45061 aacatgggca aagtaaacat ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa
    45121 atgacagcat ttgggctata aggaaagaaa actggacaat ctaaggcagg gatcgcatca
    45181 ggaactaatt tcatagctat ccctcgaaat gccggcacga tttcacctaa cagtagacgt
    45241 acaccagcta gcaatacata cacacccact acgaattgga tggcttgtaa aaaggcaaac
    45301 atgatatagt tttgatctcc tgataattca gaagcgaatg gttcgcctgc aaataaaaca
    45361 gtgatgatat atagcgggac catcacgacc atgacagaca aataagtatc ttgcagaaat
    45421 tcgaaatgtg tcggcaactt gatatcttcg atcgactttt tattttcgcc tttctcacca
    45481 aaaagcttag caacaccggc ttcaaataag tacccgatcg tacaaaaatg acctaaagca
    45541 atatcatttg atccagtgat tttacgaatg atcggttgcg ctactgccgg catagcaatg
    45601 gcgaaacaag ctccgataaa accaccgacc aagatcaata cgatccctcg cagaccggca
    45661 aaatagccaa agactgtcgt catcgttgcc atccataaaa ttgcttgacc agttaaaaaa
    45721 atgtacttcc attttgtgaa acgagccaaa ataatattga atacaaaaat ggctaagaac
    45781 gtcagtgcaa tatctctacc taaacccaaa tcattcattg cttgaccatt gatagattca
    45841 atagaaggga tgatcccttg catatgaaat ccttcagtaa agattttccc gaaataagtt
    45901 aaacttccaa cgatgatgct cgaaccggca ctcaacactt gaaatcctag gagtgtcttt
    45961 aacgtgccag agatgacttg tcctgctgat ttcttctgca gtagtagacc caacatagca
    46021 atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt cgatgataaa attgataacg
    46081 ccca
//
LOCUS       DAYYCQ010000013        44971 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000013 DAYYCQ010000000
VERSION     DAYYCQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.013"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1009)
                     /locus_tag="ACHAYF_000759"
     CDS             complement(<1..1009)
                     /locus_tag="ACHAYF_000759"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8578220.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1189..1533)
                     /locus_tag="ACHAYF_000760"
     CDS             complement(1189..1533)
                     /locus_tag="ACHAYF_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8578221.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1526..1960)
                     /locus_tag="ACHAYF_000761"
     CDS             complement(1526..1960)
                     /locus_tag="ACHAYF_000761"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8578222.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2098..2835
                     /locus_tag="ACHAYF_000762"
     CDS             2098..2835
                     /locus_tag="ACHAYF_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578223.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2828..4039
                     /locus_tag="ACHAYF_000763"
     CDS             2828..4039
                     /locus_tag="ACHAYF_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578224.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4243..5025
                     /locus_tag="ACHAYF_000764"
     CDS             4243..5025
                     /locus_tag="ACHAYF_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8578225.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5095..5757
                     /gene="trmB"
                     /locus_tag="ACHAYF_000765"
     CDS             5095..5757
                     /gene="trmB"
                     /locus_tag="ACHAYF_000765"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8578226.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5855..7609)
                     /gene="ade"
                     /locus_tag="ACHAYF_000766"
     CDS             complement(5855..7609)
                     /gene="ade"
                     /locus_tag="ACHAYF_000766"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8578227.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7741..8670
                     /gene="comGA"
                     /locus_tag="ACHAYF_000767"
     CDS             7741..8670
                     /gene="comGA"
                     /locus_tag="ACHAYF_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8578228.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8627..9676
                     /gene="comGB"
                     /locus_tag="ACHAYF_000768"
     CDS             8627..9676
                     /gene="comGB"
                     /locus_tag="ACHAYF_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8578229.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9673..9951
                     /gene="comGC"
                     /locus_tag="ACHAYF_000769"
     CDS             9673..9951
                     /gene="comGC"
                     /locus_tag="ACHAYF_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8578230.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9926..10396
                     /gene="comGD"
                     /locus_tag="ACHAYF_000770"
     CDS             9926..10396
                     /gene="comGD"
                     /locus_tag="ACHAYF_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8578231.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10368..10715
                     /locus_tag="ACHAYF_000771"
     CDS             10368..10715
                     /locus_tag="ACHAYF_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578232.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10678..11103
                     /gene="comGF"
                     /locus_tag="ACHAYF_000772"
     CDS             10678..11103
                     /gene="comGF"
                     /locus_tag="ACHAYF_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8578233.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11075..11434
                     /gene="comGG"
                     /locus_tag="ACHAYF_000773"
     CDS             11075..11434
                     /gene="comGG"
                     /locus_tag="ACHAYF_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8578234.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11606..12613
                     /locus_tag="ACHAYF_000774"
     CDS             11606..12613
                     /locus_tag="ACHAYF_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8578235.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12638..13822
                     /locus_tag="ACHAYF_000775"
     CDS             12638..13822
                     /locus_tag="ACHAYF_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8578236.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14259..15842)
                     /gene="abc-f"
                     /locus_tag="ACHAYF_000776"
     CDS             complement(14259..15842)
                     /gene="abc-f"
                     /locus_tag="ACHAYF_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8578237.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15947..16075)
                     /locus_tag="ACHAYF_000777"
     CDS             complement(15947..16075)
                     /locus_tag="ACHAYF_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578238.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16420..16563)
                     /locus_tag="ACHAYF_000778"
     CDS             complement(16420..16563)
                     /locus_tag="ACHAYF_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8578239.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16786..17265)
                     /locus_tag="ACHAYF_000779"
     CDS             complement(16786..17265)
                     /locus_tag="ACHAYF_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8578240.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17338..17622)
                     /locus_tag="ACHAYF_000780"
     CDS             complement(17338..17622)
                     /locus_tag="ACHAYF_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578241.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17700..17894)
                     /gene="ssrS"
                     /locus_tag="ACHAYF_000781"
     ncRNA           complement(17700..17894)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHAYF_000781"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17966..19252)
                     /locus_tag="ACHAYF_000782"
     CDS             complement(17966..19252)
                     /locus_tag="ACHAYF_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8578242.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19390..20061
                     /locus_tag="ACHAYF_000783"
     CDS             19390..20061
                     /locus_tag="ACHAYF_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8578243.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20027..20515
                     /locus_tag="ACHAYF_000784"
     CDS             20027..20515
                     /locus_tag="ACHAYF_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8578244.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20528..21475
                     /gene="prmA"
                     /locus_tag="ACHAYF_000785"
     CDS             20528..21475
                     /gene="prmA"
                     /locus_tag="ACHAYF_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8578245.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21476..22240
                     /locus_tag="ACHAYF_000786"
     CDS             21476..22240
                     /locus_tag="ACHAYF_000786"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8578246.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22473..24686
                     /locus_tag="ACHAYF_000787"
     CDS             22473..24686
                     /locus_tag="ACHAYF_000787"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8578247.1"
                     /translation="MTKEEILTGPGDIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24697..25143
                     /gene="dtd"
                     /locus_tag="ACHAYF_000788"
     CDS             24697..25143
                     /gene="dtd"
                     /locus_tag="ACHAYF_000788"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8578248.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25314..26573
                     /locus_tag="ACHAYF_000789"
     CDS             25314..26573
                     /locus_tag="ACHAYF_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8578249.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26662..27609)
                     /locus_tag="ACHAYF_000790"
     CDS             complement(26662..27609)
                     /locus_tag="ACHAYF_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8578250.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28003..29295
                     /gene="hisS"
                     /locus_tag="ACHAYF_000791"
     CDS             28003..29295
                     /gene="hisS"
                     /locus_tag="ACHAYF_000791"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8578251.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29310..31079
                     /gene="aspS"
                     /locus_tag="ACHAYF_000792"
     CDS             29310..31079
                     /gene="aspS"
                     /locus_tag="ACHAYF_000792"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8578252.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEDEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31188..32063
                     /locus_tag="ACHAYF_000793"
     CDS             31188..32063
                     /locus_tag="ACHAYF_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8578253.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32329..34212
                     /locus_tag="ACHAYF_000794"
     CDS             32329..34212
                     /locus_tag="ACHAYF_000794"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8578254.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34287..34571)
                     /locus_tag="ACHAYF_000795"
     CDS             complement(34287..34571)
                     /locus_tag="ACHAYF_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8578255.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34561..35295)
                     /locus_tag="ACHAYF_000796"
     CDS             complement(34561..35295)
                     /locus_tag="ACHAYF_000796"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8578256.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35424..36047
                     /locus_tag="ACHAYF_000797"
     CDS             35424..36047
                     /locus_tag="ACHAYF_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8578257.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36239..37360
                     /locus_tag="ACHAYF_000798"
     CDS             36239..37360
                     /locus_tag="ACHAYF_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8578258.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37367..40495
                     /locus_tag="ACHAYF_000799"
     CDS             37367..40495
                     /locus_tag="ACHAYF_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8578259.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40514..43708
                     /locus_tag="ACHAYF_000800"
     CDS             40514..43708
                     /locus_tag="ACHAYF_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8578260.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43854..44561)
                     /locus_tag="ACHAYF_000801"
     CDS             complement(43854..44561)
                     /locus_tag="ACHAYF_000801"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8578261.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14443 a         7977 c         9420 g        13131 t
ORIGIN      
        1 attttgatga atcagttgtt tcttcagttg aagattcttt tgttgaatca gttgattttg
       61 tgtcagaact ctttgttgaa gctgtagttt cacttgagtc tttcgtactt gatgaagttg
      121 ttgtgaatgc actcaacaca ttttcaaagg catcatcttt gatttttaca tttgcatctt
      181 ttaattcttc accgattact ttagttgtga atgcattgtc acttagttta gtttcagtag
      241 cgatatcttt tagttgatct ttgtatttat ccatgtcatt gcctttattt tggtttttca
      301 ccattttgac aacgtagtat tctgtcgcat aagaagtagg atttgttgta gtaataacat
      361 cggagatctc accgtctttc aatttgaaag ctgcttcttt tacttctgca ggaatagttg
      421 ttgtcgttga gtcgaattta actttaccgc catcttcttt tgtctctgta tcagttgatt
      481 tgtctttggc taatttagag aaatcatctc catcatccgc tgattttttc acatcttttg
      541 cttcatcttc acttgataat ttgatgattt gtgcttctac ttctggatgg aaagatttcc
      601 aagcagtttt caaatcgtca tctgtaatat ctacgtgagc tttcaagcca gcttcaaaag
      661 ctaaattgtt acgaatatat tctttataag tatctttcgt atatccagca gcttctaatt
      721 gtgattcaaa agtatcacct aatgatttcg cttgtttgtc gtattctgca tccacttgct
      781 tatcggttac tttatcgcca tatttgttat tgaatacttt ataaatgatc atccgttgta
      841 ctaaggattg gttcgatgat tcaagttttg cttcatcata gaaatcagat acagtgatcg
      901 ttccgccttt catcgttgcg atatctttat tagtatcgcc tgaacaagct gctagagtta
      961 aaacagccaa cgcactagta gctgccaaaa taattgattt ttttttcatt aaaatttgca
     1021 ctcctgtaat tagttattat ttttatatat taactatacc atatcccatt tcctttaaaa
     1081 acgttgtcgt ggtagtttca aacaaaattc aaaatttcat cacattccta ggaaatacgc
     1141 aaacttttct cacttatata gttaaatctg ttgagaaaat aatcattatt atatttgttc
     1201 aggatactct ggcagatcag cttttagttt ttctacttgt tcattgattt gtgtgatacg
     1261 tggttcggct tggaatttaa aatcttcgat atcttttttg attgcttctt ggaaagcagg
     1321 aatcacagat tcagctgttt cttttgtttc gatcaatgcg cttttaaaat gattcaggct
     1381 gttatttaaa tcatttgtca gagttgttgc ttcttccaat tcttcgatca gtttctttct
     1441 tgtttcattc ccgctcctgg gcgccaataa cagacctccg acagtgccag cagcagctcc
     1501 aaataacagt cctttgaaaa agtttttaat catcgcagga cacctgcttt acgattgttt
     1561 cttgaatagc ttttagttct tccgatccat aactatcttg atgattgaca aagtgaatag
     1621 aaaaatcatc ctctttgctg tagcgaggga ctaaatggat atgtgaatga aaaacagatt
     1681 gataggctag ttcacggttg ttattcaaga tattaagacc tttcatttct ggaaaagctt
     1741 tttccaatgc ttgagcaatc ttaggcacac gcgaaaaaac ctctcctgca agttcgggat
     1801 catattcaaa aatatctgct acatgacgtt taggcacaac taatgtgtgt cctttcgttg
     1861 cttgagagat gtctaaaaaa gcatagacct tgtcgtcttc atatatttta tagcttggga
     1921 tttcttgatt gatgattttg caaaaaatac agttttccat ataaaacatc ctttctataa
     1981 agaataaata cttcagctta actttaccat aagttaaagc tattgtaagg aaaaaaacaa
     2041 aataccgtca ttttcttcgt ggtataataa ataaaaataa atggcggagg aaaaagaatg
     2101 agtttaatta tcgatcatgt cacagggggg tacggacatc tccctgtgtt aaaagatatc
     2161 agtttcgatg tacagcctgg agagatagta gggttgattg gactgaacgg agcagggaaa
     2221 agtacgacca tcaagcacat tatcggtctg ctggaagctt caaaaggaac gatagaaatc
     2281 aatcatctat ccttaaagaa aaatacggaa gaatatcgca aacaaattgg gtatattcca
     2341 gagactcctt ctttatatga agaattgaca ttgagagaac atatcgaagt cactgcgatg
     2401 gcctacgata tacctatgga tatagcaatg aaaagagcag aaaagttgtt ggagactttt
     2461 cggttatcta atcgtcttga ctggttccca gctaattttt caaagggaat gaaacagaaa
     2521 gtgatgatct tatgcgcctt tttgatcgag ccttctttat atatcatcga cgaaccattt
     2581 ttaggcttgg atccactagc tatcaatgca ttgcttgaat tgatggtaga aatgaaaaaa
     2641 gaaggagccg gtattttgat gtctacgcat attttagcta ctgcagaaaa atattgtgat
     2701 cgttttgtgg tcttacataa cggagagata cgagcagacg gttctcttga gatgttgaga
     2761 aaagaatttc agctgccgga ttcttctttg gacgaaatct atattgcttt aacaaaggag
     2821 caggcacatg agtagttttt ttcaaaaacg tttagcaaga catcaaaaga aaatgatgaa
     2881 atatatgcgt tatgtattga acgatcattt tgttttagtt tgtcttttct tgattggcgg
     2941 tgtgggatta tattattcta attggttgaa aaccctgcag ccaccgtttg caattggagg
     3001 gctcataatc tctgtatttt ggatgctctg tttattcgtg ggtaagatgg ctacttttgc
     3061 agaggcagca gatattgttt ttttacttcc taaagaaaaa gaaatgcaaa cctatttgga
     3121 acgagctttt cgctattctt gcatttttcc gttgatcttt ttgatattgg catgcggatt
     3181 ggccatgcca ttagtggtgg ttagtacgga gcaatccttt tcgatgtttt tcgtctatct
     3241 gatcatatta tggagtttga aattcagtca tctgcaaatc aagagagcag aattgtttcg
     3301 actagatcaa caagttattc gttattggaa attaggctgg tttttttctt ctctgatctt
     3361 tttgtttcta tcattttatt ggctaatatg ggcaggaata gcaggtgcat tagtacaggc
     3421 aagtctatat tactggtttt tgtggaagca acaagaaaaa ggattggatt gggagaaaat
     3481 ggtgcatgta gaagaacata ggttgcatca gatctatcaa tttatcaatt tattcacaga
     3541 tgttcctgaa attacggcaa agatcaaaag aagacgctat ttggatccta tattgactca
     3601 tattaaaaga gaatcaaaaa acacttatct ttacttgttt gcaagaagat tggcaagagg
     3661 ttctgatttt agtggtcttt atttccgatt agtaattata ggtgggttat tgattgccgg
     3721 agtagtcgac ttttatttta gtctgattat cggtttctta tttttatatt tgatcggttt
     3781 ccaactgtta cctttagcga accagttccg ctatatggtt ctcgttcagc tttatccaat
     3841 ccataagaat cagaagaagc aggcgattca gtatctactc acatgggtat taattatcgc
     3901 gtcggttatt tttggtttga ttgctgcagt cgtattagat gggcagcagc gatgggttcc
     3961 gctagcggct tatttgattg ttactatgct ctttgttaaa ctatatgtac caactcgttt
     4021 gaagaagatg tcagactgaa aacaaagctg tgtagggtaa aaatgagaat gaaaaataga
     4081 gcgttgacag taagaatgct gttcgttcta ttttttttga gaaaagctgg ttttttgaat
     4141 ggcaattaaa tgaaaaagaa cgtatatgtg cttgacgtgt agtgttcagg cgactagaat
     4201 aataagtact gcaaaacaaa gagtaagagg acgggagaat gcatgatgga gtttcagtta
     4261 gaccaagagt ggcggttacg tcccatcaaa ggcgacacag gaaaaacata cataggcatg
     4321 cgtgatgagg acaaagtctt tattaaacga aacacgactc cgatgttggc tgctctttca
     4381 agggaaggaa ttacaccaaa acttgtttgg acaaaacgaa cggggaatgg cgatactttg
     4441 acagcacaag agtggctgga aggtcgcttg ctgactccta atgagattgg tcaaagaaac
     4501 gacgtgattg atgttttgta ccatttacat cattcaaatt ctcttaaaac catgctgacc
     4561 cgtatcggtg gtcgtgtcat gacgccggag aagatgctgc aaaaatatga aaaagaatta
     4621 ccggaagaac tccagaaaaa tcaatttctg caaattgtcc atcgttttct tcgtgagcag
     4681 atcccgtcaa tagataagga aaacttatca gtagtacacg gagatgtaaa ccatcgtaat
     4741 tggttagtat gtaaaaatta tttatatcta gtcgattggg attcaatcat gtttgcagac
     4801 cctgcggtgg atattgcaac gattttagga aattatgtgc cactgtctag ctggaatcag
     4861 tggctgatca gttatggcat acgcccgact gacgaaatgc ttgaaaaatt tcattggtat
     4921 gcggccatga atttgctcca agaaatcacc agatattgca aacgtgaaga tcatcgacgg
     4981 atgaacgaag aaattttaca attaaaacga attttcagtg gctgaggttt ggcccaaact
     5041 ctgcggagat ttggccagac ctttttaatt agaggaaagg aatggagata aataatgcgt
     5101 gtccgaaatc gtccgggagc agcggaaatg ttagcggctc acccgaattt tgtaattagt
     5161 gatccaacat tgtggaaggg aaaatggaat gaattgtttg aaaacgatca tccaatccac
     5221 attgagattg gtatggggaa aggccagttc atcactggaa tggcaaaagc ccatcctgaa
     5281 atcaattata ttggagtaga aatgcaggtc agtgttgttt cgattgcact agacaagttg
     5341 atcgaacaac cgcttcctaa cctgaaactt ctacatgtgg atggatcagc gttgacagag
     5401 tatttcgctg acagcgaagt agatcaaatc tatctgaatt tttccgatcc atggccaaaa
     5461 aaacgtcatg aaaaacggcg attgacatat aagacttttc ttgctgtaga tgaacagatt
     5521 cttcgtccaa atggagaaat ccatttcaaa acagataatc aaggattgtt tgagtattct
     5581 ctggcaagtt tttctcaata tggcatgatc ctcaaacagg tatggttaga tttacatcaa
     5641 agtcaatttg aaggcaatat tatgacggag tatgaagaaa aattttcttc aaaaggtcaa
     5701 cgtatttatc gagtagaagc tcaatttcaa gataagacca aaaagaaaag tctgtgaaac
     5761 taactttctg ctttcataaa aacattaata tccataaata aatcccagaa aatgaacatt
     5821 acagatgtaa tgtattttct gggatttatt tgatttaaaa tgtatttatt tcatcggttt
     5881 ttccacaagg tggaatcaat tggaagcgag aaaatgaaaa taatcctttg tcggtgatct
     5941 tcaattcggg tatgacagat aatgttaaga aagaaagtgt caaaaaagga tcaaaggttt
     6001 tagctgctcc tagttgatgt gctttagtta ttaattcatt caactgttca tagacgatcg
     6061 aaaaaggttc ctgtgtcatg attccgccta taggaagaga aacacaagct atttcctgct
     6121 gattgttgac aacggtcatc ccgccacctt tttgaatcaa aagattagca gcatacagca
     6181 tatcctcgtc gtttgtccca acgacaacta gattatgcga gtcatgggca atcgttgtcg
     6241 ctatcgctcc ttgtttcagc tgaaatcctt tcactatgcc taatccaatg tttcccgttt
     6301 gatggtgacg ctcgatcacc gcaatcttta attggtcttt ttcgattgaa ggaatgaatc
     6361 gttcgccttg tcggtccact ttttctacca aatctttagt cactagacta ttcgggataa
     6421 tttcgatcac atgggcttgt gaagaagaaa gaagcagctc taacgaatgt ggatttaatg
     6481 gttgagcgtt tagttttggc agacaacacg tatatgaatt tgaatgatca gcaaataaat
     6541 ggtcttggag ttgcccagat tgaacaacta atgttccctt atagaaaact tcatgaattg
     6601 gcagcttggt aagatcgtct gttaagaaga aatcagcttg atacccaggg gcaatcgcac
     6661 ctagtgtttt caaatcaaaa cattctgctg cattcaatgt tgccatctga atggcttgta
     6721 atggatcgat tccaagtgca atagcttgac ggacgatatg atcgattgat ccttctttta
     6781 ctaaatcatt gatcagttta tcatcggtga caaagaagca tcttcttgaa ttttcaggtg
     6841 tgatcgccgg tagtagcgat gccagatctt ttgcaacggt cccttctcga accattaaat
     6901 acataccaag atccaaacgt tctttagctt cttttgcagt tgtagcttca tgatccgtcc
     6961 gaatacctgc tgcaggataa acatttaact cttccatgcc gataccagct gcatgcccgt
     7021 caattttttt cttatgctgg tgcatcaatt ggatcttttc taggatgtct gtttcattat
     7081 ttgctactgc ttgatagttc atgacttcag ctaagcctaa aacttcgggt ttttgtaaaa
     7141 aaggggctaa tttttttgca tttaaatttg caccattggt ctcaaaaggg gttacaggta
     7201 cacaagaagg tagcataaaa aagatattca tgagtgtttg tcttgcatct tctatcataa
     7261 actcaagacc tgtactccca gcaatgtttc ctatttcatg gggatctgtc actacagtag
     7321 taactccatg aagcaaggat acttttgaaa attcgcttgg tgataaaagt gagctttcga
     7381 tatgcacatg gccatcgatg aatcctggca ctagatattg cccattagca ttatgtacgc
     7441 tttttccttc gtattcaccg attcctacga tcattccttc gcagattgct acatcttttt
     7501 ccatgatttc tttcgtaaag acgttgacga tcttagcatg cttgatcact aggtcagcag
     7561 gctttttttt acttgctact tcgatatatt tccttaatgt tgtttccaat ggagtgcctc
     7621 ccaagataaa tttttataag tataacataa attcggtttt tgccaatatt tttttgtaaa
     7681 ttcattttat ttgaaacaaa aagatcaaaa agacgtatgt tttttgtgga ggggagaata
     7741 atggaaataa aacgattatc ggatcaaatg ataagtgaaa gttttgccaa acatgcccaa
     7801 gacgtatata tattgccagc ttcagaagta ttttacatct attttcgcta tggaaaaaaa
     7861 cgaacattct ggaagaaagt ctcttcggca gatggacagc aactgatctc aagatttaag
     7921 tatttgggac agatggatat cggagaaaaa agaaaagctc agttaggtgc atttacttat
     7981 tctttagaaa aacaggaagt tcgtctgcgg ttgtcttctg taggagatta ccttcaacga
     8041 gagagtttag tgattcggct attatacggt acttttgaaa caccccatcg ttgctttagt
     8101 tctcgagatt ttgcaactat tttacagaaa accgtcagac gtggattata ccttttcagc
     8161 ggaccagtcg gatcaggcaa gacttctcta atgtatcggt tggctttgga agaaaaattg
     8221 caagtgatta ctatcgaaga tcctgtagag atagaagaac cgttttttct gcagctacag
     8281 atcaatccga aaatagcaca gacttatgat acacttttga gactcgcatt acggcatcgt
     8341 ccagacttgc taatcattgg cgagataaga gatgaatcaa ctgcacatgc tgcggtaaga
     8401 gcagcactaa cagggcaccg ggtttttgca accgtacatg cacgcaatct tgaaggtaca
     8461 ttggtcagaa tccatgaact gacaaagaaa aaagaagagc tatctgaatg tttagctggt
     8521 gtcatttatc aagaactttt ggaagatgcc aatcaccatc cttctgtttt atggagttat
     8581 caatttatgg aggaagaatc agtaaaaaaa tcatgggagg aaagttatga agaagctcag
     8641 gaaaatattt ggcagtcaga actattctaa aggattaaca agaagccagc aaaaccagtt
     8701 tgcacaaacc atgggagaac tgcttttatc tggttttagt ctgcaagaat ccctttattt
     8761 attactcacg atccgttcgt ttccacctgt tattttggca aatatccaac atcagctaaa
     8821 ggaagggcaa gcgtttagtg aggtactttc gcaaatagga tatggagcag agcaactggt
     8881 acagatcgag ttagctgaac gtcatgggaa tctgccgaca actttgttgg aaattgccaa
     8941 acagtttcgg cttgttgaag gatttcgcaa tgatttgaaa aagctgcttg catatccgat
     9001 gctattgctc acctttttgc taggtatctt gtttagtatg cggttgttcg ttttgcctca
     9061 gcttattggt tcaggaatgg tcatgcaaga gcattggggt attcagctga tacaagtctt
     9121 tcattggtat gttctactat tcatcctagc ggttactcta ttggctttac tagtaaggta
     9181 tcgtctgatg aagttgtctc ctgttgatca gagtgaatgg ataagcagac aattattgat
     9241 tggaccgata tattccttgt atcaatcggc ttatttgtca ctagagattg gaaagctttt
     9301 tcatgaaggt ttcgaattaa ggcaaatcat agtctgctta aaggaaacga aacaagaaag
     9361 tctgattcag catcttgcct ctaagttgat tcaaggattg gaggcaggta tgtcattggc
     9421 tgagcagttc cagaattatc gttttctatc agaagaattc agtaaaattg ttttacaggg
     9481 agaggcaaag ggaagtttag gtaaagaact gattttttac agcgaatgga caagaaaaca
     9541 gcttttcaaa cgtcttcatc actggatgca ttttattcag ccaatcattt ttttgggcgt
     9601 agcggctttg attttattag tttatgccgc tgttttatta cctgtttacg gaagcataga
     9661 ggaggttttg ccatgaaaaa gagaaaaata tcttattata gcggttttac attgatcgaa
     9721 atgctaatcg tcttactgat catcagtgta ttagtgttgt tgttcgtccc taatttgtcg
     9781 cgttatagga atcatgtaga ccaagaaagt cgtgaagcga tcatccagtt ggtagacaca
     9841 caaaaagagc tctatgcgct tcaaaataat ggaagagttc ctactgtgga ggaattgttg
     9901 aatgaaggct atatcaaacg agaacatgca gaaatctatc agcggccttg aaggtaaggt
     9961 tttagcatca ggatatacgt tgtttgaatc actcttactg attttattac ttacaacgat
    10021 cctttctttt ccagttctcg cattttctgc atggaagaat gaattggcag tctatcaatt
    10081 tttcggccaa tttgaaaaac gaatctattc tacgcaaaaa atcgctattg tgaatcagat
    10141 ccaaacaggc ttttattgga atagtgaaga gaatcaaatc atttttcatg ttccgaatcc
    10201 agataaaata aattgggaag tattagatat tcctgatgag attaccttga aacgccatgc
    10261 ttctattact tttgctgcta gaaccgggaa tgaaagttca ctaaaagctt atcaatttta
    10321 ttgggaagaa aaagagcgga cgattacgta tcaattccag atagggagtg gtcgctatac
    10381 aaaaagaatt gaataaaaca ggatttgttt tgttggagtc ccttacagcg cttatgatcg
    10441 cttctgtctg tgtctactta gtgagctttg gccagctgtt tttgcttcag gcagaaaaga
    10501 aaagtgggga ggaactgcta tttattcgaa tgatttatga agatatcaag acgcagcgga
    10561 tgtatcacaa aagaccagag cgaagggaag aaatgatccc atacgatatc caaattgtcg
    10621 ataataatgg agtaatcgaa aaagtgtttg tcaatatgga gaaagaagcg atcagtattg
    10681 aaaaaacaag taattccagc atttacgctg attgaatgtc taattggact gatgatattg
    10741 agcagtctat tgctcacatt caatcttttg attcagcaaa ccatccaagt aagtgaattt
    10801 ttgcagcgta aagatcaaaa agagtggcta atatttttag cacaattaga agaagaactc
    10861 aagagcagtc acaatgtctc tgtgaaaaat cagcaactgc actacacgat cgaggataaa
    10921 cagtatatca tcgaaagata tcaggcaatg atacgtaaaa gaagaacttc cggcggacat
    10981 caacctatgt taacaagtat cactgagctt caattattgg aaaaagaaca cacaatttct
    11041 ttttatgtac actttgaaaa tggagaagat ggatatgcca tatggacaaa aaacgatcag
    11101 tagaaaatgt tataagggct ctattttatt ttctgtcttg cttttatttc ttttatgtac
    11161 gtttttattg atggcagcat tagaaagcta ccgtttgtca atggatctta atatacgaat
    11221 caaaaattat tatatagcaa aaataatgaa gcagatggct ttatcggaac agtctacgct
    11281 tgccgaaagt tcggaaggag ttttttacta cacaactgga tctgtaacat atcagtgggt
    11341 acaacagtcc ctattcttag aggtggaagt ctcaccgttt atctttcggt tcacagaaga
    11401 agtaaaaaat gatacagata ctagtacaga atgatggaat gttcgtgaaa acgccctctt
    11461 ttctgttata acactgtttt taataaaaaa atgtttgccg tttcacaaat aaaaaacagt
    11521 gaaaacgatt gaaattttgt caattgttcg atagaattaa acaggtgaaa aaattgttat
    11581 aatatatcta gaaatgaggt gcaccatgtt accagaaaaa atggaacaag ggtttaatca
    11641 aaacctggaa gctattcagc ttttacagaa tgcattggga acttcctttt tagaagccta
    11701 tgtcgaaaat gcagaaaatt taatagatga ttatcaagta cgtgtggtgg atggagtgcc
    11761 gacaaaagag acaacgcaac ggatcacagc tctttatgaa gagttgaaga aactttcttt
    11821 cgagccagaa gaatggcgca gactgtctca attactttta ttgaagggca gccagacaga
    11881 acatttgcag gcgaaccatc aactcactcc tgatagtatc ggttttttat ttgtttttct
    11941 tattgaacaa ctatacacaa ataaaaaagc accggttaag attctggata ttgcggcagg
    12001 aatgggcaac ttgttattaa cagttctttt aaacctatcg aatgcggggt accaaacaga
    12061 aggaattggt gtggatattg atgatacatt gttggctgtc gcagcttcta caagtgattt
    12121 gacacaagca aatgttcaat actttcatca agacgggttg caggaattat taatagatcc
    12181 tgtcgatttt gcaatcagcg atttgccaat cggttattat ccgaatgacg aaaaagcaaa
    12241 agagttttta acaagtaccg aagaaggcca tagttatgca catcatttat tgctagagca
    12301 atcaatgaaa tatgtgaagc cggatggttt tggtttgttt ttgatgccga gtggattttt
    12361 agaaacggat caaagtgaag aaatcaaaaa atggtttaaa gaagaaggtt atcttcaagg
    12421 aatgattcaa ttgccagatg aattgttccg taataaacaa tcgcaaaaaa gtattttgat
    12481 cttacagaaa aaaggaccac aagcacaaca agtaaaagaa gtcttgcttg tgaaacttgc
    12541 ttcattaaaa gaaccagaga aggtgacgga atttttcaac gagtttaaaa actggaaatc
    12601 atctgctctg taatcattca acgagaagga gagtcatatg tctaaaacaa ttgcaatcaa
    12661 tgctgggagt tcaagtttga aatggcaatt atatcaaatg ccagaagaaa ccgtagttgc
    12721 gaaagggatc gtcgaacgta tcggtttaca agattctatt tttaccatca agtacggcga
    12781 taatcaaaaa ttcgaacaaa tcatggatat cgaaaaccac gaagtagcag taaaaatgct
    12841 gttagatcag ttgattgaac tgcaaatttt aagctcttat gatgaaatca ccggagtagg
    12901 gcaccgtgtt gttcacggag gagaaactta tggggattca gtagtgatcg atgagaaagt
    12961 aatggaccgc attgctgaat tagcagaatt tgctcctttg cataatcctg caaacttaat
    13021 ggggatcaaa gcgtttaaaa agattttacc tgacattttg agtgtggctg tattcgatac
    13081 atctttccac tcaacaatgc caaagcaaaa ttatttatat agcttaccgg tgtcttatta
    13141 tgaagatttc aaagtaagaa aatatggctt ccatggcaca agtcatcgtt atgtgtcgga
    13201 acgtgcggca aaattattga acaaaccgat cgaagaacta aaaatcatca cttgccactt
    13261 aggaaatggt gtatcgatca cagcagtcga tggaggtaaa tccatggata cttctatggg
    13321 attcacacca ttagctgggg tgacaatggg tacacgttca ggtgatatcg atccagctgt
    13381 tctcccatat ttaatggaaa aattagagat tgatattgat gaaatgatca atgttttgaa
    13441 caagaaatct ggtctgctag gcctaacagg attgtctagt gatatgcgtg atcttgaaaa
    13501 gaactatgaa aaagaacata ttcgtttagc ttatgacatt ttcgtggacc gcatccgaaa
    13561 atacatcggc ggttatgtga caacgatgaa tggtgttgat gcaattgtct ttactgctgg
    13621 tatcggcgag aatgacggac atgtacgtag tgaaatcatc aaaggtatgt cttggtttgg
    13681 ttgtgaaatc gatccagcgt tgaatgaatt gcgtggtgaa gaagtagata tctctacaaa
    13741 agaatcaaaa gtaagagtat tagtgatccc aacagatgag gaactaatga ttgcccgtga
    13801 tgtggagcgt ctgcgcggct aagccaaagc attcgttagg ataaaagatg aaacgcatgc
    13861 aagagggaat tcttgcatgt gttttttttg cggagaagac cgcaaaatgg taatttaact
    13921 tcaatttccc ttctttttta caggatcttc ttagggaatt ttgcttggaa aagtggtcaa
    13981 aagggaaaag actggcattt tatttattga ttttcaggaa aatcccaata ttttattttc
    14041 attcatcttt ataagggaca ttacatatgt aaaaagaaaa aacattttta gatcagcctc
    14101 gtgggctgtc gactttattc ttcgctttat gtttagctag aaaaccgatc cgaaacattc
    14161 attgatcgaa taataaaaac ttgattttcc acctgtactt tgcctgaatc gtgaaagcac
    14221 aggtatgaaa atcaagtttt ttgttgcgaa aaaacgtcct agtcaatcag ttggccgagc
    14281 ggctggcggc tgatctgctc ctgatcaata acgagtgctt cttggaagtg ttgctgcaaa
    14341 aagtagcggt catggctgac tgctaggagc gttccttgat agtcttgcag catttcttca
    14401 atttcttctc gagcatagct gtcgaggtga ttggttggtt catccaaaat caggacgttg
    14461 attttttcta aaaagagttt cagcaacgcc aaacggattt tttcaccacc ggataaatct
    14521 ttgatccgtt tacccacatc gctttgataa aagccacttt tggctaattg ccgtcgagcg
    14581 acttcttcgt ttccagtcaa tgtgatggcg taagccagca aacggctatc gggatcggca
    14641 aactgcagct gctgcggcaa ataaccgatt tgcaagctac tacctaattt gatggtgcca
    14701 ccgtaaggct tttcctcacc gagtaataat ttgagtaagg ttgattttcc tgcaccattg
    14761 tcgccgatga tggctaaatg atctccacga tagactgcaa aactgctgtc tttgaatagt
    14821 acttgatcag cgtactgctg gtgcaagttt tgaatgatga aaacttcttt gccagatttt
    14881 ccaccattag acaagctttg cagccgattt ttcgggggag gtggcggttt gacgagtgtc
    14941 agtttcgcca ggcggcgttc cagttctttg gcttttttga agaaggactc attgtctcct
    15001 tcatgtgccc actgacgaaa acgacgaatc atgactttca accgctggac ttctttttgc
    15061 tgcagttcgt aattttttgt cagctcagcc aagcgagctt ctttcaagac tcgaaaacgg
    15121 ctgtagtttc cgggatactc aatcaatgca ccttcttcca gttcccatgt ttttgtggtg
    15181 acttgatcga gaaattgccg atcatgagaa acgaccagaa ccgatttttt tgtattctgt
    15241 aaatagtttt ccaaccagcg aatactgtta agatctaaat gattggtcgg ttcatcgagt
    15301 aaaagtaaat cggcatcggc tgctaaaatt ttggctaaag cgactttaac tcgttcgcct
    15361 ccgctaagtt ctgttaaccg tgcttcctga cggtgtggaa tccctaagcc ttgcaagatc
    15421 gctgcaatcc ggtcttctaa tacgtaaccg cctagttctt caaagcgatg ctgcagctca
    15481 ccgtaacggg ccagacactt ctctaagttt gtatctggtt ctgtcatcaa tgcttcgtag
    15541 cgactcattt gctgtttgag gtcctgtatc tcagcggcag aatggagcaa gtagtggttg
    15601 acggtcgctt cggaaactgt aagttcttgt tcgaccgtcc cgatctttaa gccttttttg
    15661 cgactgataa caccgctgtc gacaccttct ctgccggtca agatttgtaa aaaggttgat
    15721 ttgccagttc cgttgatacc gactaagaca attttttcac cagcttcaat ttgacaggat
    15781 aaatcctcaa ataatggatt cccgccgatt gtttttttaa tagaaattgc ttgtatgatc
    15841 atgatctctc tccttgagac cactcaaaaa ggccatggtt atccatggcc tgtgttattt
    15901 aaactcagat tatcatgaga aaaatacctt gagatggcca ttgattttac gtgaattcaa
    15961 ctgttttgga caaacttgtc ccgtcaacag caactgtaaa accaattgca tggctaaatg
    16021 cgatatggta tcgttggtaa ttttcattac ttgttatttt agtcatgcgc atcatagtat
    16081 tttccctcct tcgtgtagtt gtcttttcca gtgtacctga aagtgtcaga aagttcaact
    16141 agcgctgaca tttacttaat atttatcgat gtgtaattct gtcagagagc tttttttatc
    16201 tgactgaggg taatgtctcg tggaccacat cattgtattc tgagctaaac cagcgttcag
    16261 gcgaaatcat atggtgtttg ataaaaaact ttgtctacat gacttccttt tgccatttgc
    16321 ttaataccgt tgcattaaac agctcttctc tttggaaatc tcaccaacat cgcgttcagg
    16381 agtatcgttg tggttgcaat ggcaagcggt agcctttcat caattcttga atgatttacc
    16441 gaatgtagca cttgccttct cgcggtaccc gaacccgaaa taaaatttta gtttggcgat
    16501 aaaatcggta tccgccatcg atacttcata ttttttcgta tcagattcat cggaaacttc
    16561 cacattgacg aaccaataag ctttggcatg cttaggtcgt tttaaaccac accagggcga
    16621 ctaatcgccc tggtgttcac ttttttttat tttacttgaa ctcattgact gttttccctt
    16681 tattttaaac attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt
    16741 ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg
    16801 gtctttagct ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc
    16861 gtttctaata acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc
    16921 tgtggctcct aacataatca aatctacttg attatcttct gggatttctc tggcaataat
    16981 ttgttttggt gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc
    17041 attgctttca taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat
    17101 tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag
    17161 ttctccgtta tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc
    17221 agaaccatct acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc
    17281 aagtcaatcg tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta
    17341 ctgctttggt tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat
    17401 tacgatcagt aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc
    17461 cattacgcct aaaacagcat atgcagctcc gtaaaattgt aggaatcctt gatttttatc
    17521 atttttgttg atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa
    17581 taataatact aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat
    17641 aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg
    17701 taatctccgt taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg
    17761 ggtcccatgg tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca
    17821 agacgggtcc caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa
    17881 catcacagat ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct
    17941 cgcagaaaca gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta
    18001 tacggtaata ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat
    18061 actgagcatt cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat
    18121 gatgaggata ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat
    18181 ctctcaaatg atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat
    18241 ctaatgccat gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg
    18301 ctagtgggat acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg
    18361 tacgagccgc agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac
    18421 gccggcaaat acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag
    18481 catccacatc acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat
    18541 ctccattttt gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg
    18601 taagatgaat ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat
    18661 tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt
    18721 ctaactggat tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg
    18781 ccaactggat gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga
    18841 tcgctggatt gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc
    18901 cactttctaa atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat
    18961 ccaataagag gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct
    19021 tttttgaatc tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg
    19081 cacttgcgat actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca
    19141 tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa
    19201 cgacttcatc gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt
    19261 cctccttctt gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa
    19321 cagccttctg ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag
    19381 gtgagaaaaa tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca
    19441 gaagtggcac gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta
    19501 gctggatata ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc
    19561 tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac
    19621 ctttacacga tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct
    19681 caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat
    19741 cgtggcggaa aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta
    19801 gcaattcctc aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt
    19861 gagaaaaaaa agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa
    19921 ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta
    19981 caaagaaaaa accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga
    20041 tctattaaca tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt
    20101 agattgggat acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa
    20161 agcacaattt gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt
    20221 tgaagacggc gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct
    20281 agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt
    20341 ggaatatcta aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga
    20401 agacgatgac gaagctgtat tcgagcttca ctgggataat cagcaactag cagaattagt
    20461 agaagtaaaa cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt
    20521 gacagatatg aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt
    20581 tgctaatatt ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt
    20641 tgaacatttt cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct
    20701 gaaagaaggc gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt
    20761 gccttttatc aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa
    20821 taaagtaaca gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta
    20881 tcatccggtg cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac
    20941 agaagatcct ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac
    21001 tcatcccacg acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac
    21061 attgctagat gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc
    21121 aaaagatgtc tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat
    21181 ggatatgaat gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat
    21241 cgagattgaa agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat
    21301 cccagatgct tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga
    21361 agagaagaag caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat
    21421 tttccagcaa aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca
    21481 gcggtatttt ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa
    21541 ctatcaccac attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt
    21601 caatgatcaa atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct
    21661 gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag
    21721 tggctatcct aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc
    21781 gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg
    21841 tatgaaacga caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg
    21901 gcagatcgtg ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga
    21961 tgattatgac gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa
    22021 tgcagccaaa attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc
    22081 agaaggtgga ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg
    22141 tggattagga ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc
    22201 tagctatcaa tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga
    22261 gtatcgacag aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta
    22321 tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa
    22381 aacgtataac tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt
    22441 tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac
    22501 ccggtgacat aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga
    22561 aagcatgcga atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct
    22621 acattattca tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata
    22681 cagtggctac aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc
    22741 tgagagaaga atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga
    22801 aaataaaata taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag
    22861 caatggctca agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc
    22921 gtacgttgaa gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa
    22981 tctatgcgcc tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata
    23041 cagcacttcg ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga
    23101 agagagaaga acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag
    23161 aagagctggg gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc
    23221 gtaaaatgaa agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag
    23281 tgatcgtaga ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga
    23341 aaccaatgcc cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat
    23401 ctttgcatac taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac
    23461 aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa
    23521 aaaatgaaaa agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac
    23581 tcgaacttca agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata
    23641 tctttagtga taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag
    23701 gatccggacc attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg
    23761 gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca
    23821 ttgagatcat gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg
    23881 ctactagcaa agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa
    23941 atgtgatcaa aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta
    24001 aagatatttt gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag
    24061 aagatgatct ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc
    24121 gtctgactga aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag
    24181 aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg
    24241 ttgtcattca aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc
    24301 ctggtgatga tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag
    24361 attgtccgaa tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat
    24421 gggaagatac atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata
    24481 atcgttcagg cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca
    24541 acagcgttga agcacggacg aataaagata aaatggcgac gatccatttg acggttggta
    24601 tccagaattt atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct
    24661 acagtgtacg ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta
    24721 agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt
    24781 gttttattag gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa
    24841 attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat
    24901 gtgggaggac aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga
    24961 aatcgaccaa gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca
    25021 ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat
    25081 atgtcggttt ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa
    25141 taaagtaatc tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat
    25201 aaactctaac ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga
    25261 aaaaaagtta agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag
    25321 aagtaccagt agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca
    25381 acatgttgga ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca
    25441 ataaagaagc tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga
    25501 atttcaaacg aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg
    25561 gagtacagac tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa
    25621 tgacaccgga aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac
    25681 ttgaagcaca gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat
    25741 ctatttgcga gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat
    25801 tagcagaata tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa
    25861 aaggtgtcga aaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc
    25921 gacaaaatcc tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag
    25981 atttagatgg tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc
    26041 gtccaacggt tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac
    26101 cagatttgta tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag
    26161 aaatgagacg ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag
    26221 aagtagatgg ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag
    26281 accatgatga acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga
    26341 aagcaaaaga tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt
    26401 ttcgacaaat gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt
    26461 ccgatcagct agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat
    26521 tcaatcagtt tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca
    26581 taaacttatc gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta
    26641 taaattgata aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc
    26701 ttcattggat aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt
    26761 agctccaggt ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg
    26821 cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat
    26881 ttctctgaaa cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa
    26941 ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata
    27001 gggaacaaaa ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg
    27061 atggatcaaa ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt
    27121 caattgttct aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa
    27181 ttcgtgcaat gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata
    27241 aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag
    27301 aaactcagga gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc
    27361 gatgacgaca cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc
    27421 ggaacggccg tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc
    27481 tgtacgaact acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc
    27541 aacggcattt gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat
    27601 ttctgccata gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa
    27661 ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta
    27721 gtaggttata agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca
    27781 agagaggatc atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga
    27841 gatggacgag tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc
    27901 tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg
    27961 gtgatttttt tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca
    28021 aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca
    28081 gcacgtttat tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac
    28141 tatgaagtga tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat
    28201 gatttttatg acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc
    28261 gttcgttctt ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact
    28321 ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc
    28381 catcaaatcg gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg
    28441 gtgatggcac ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat
    28501 tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta
    28561 ccttttgaat cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc
    28621 gtattggaca gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta
    28681 gattatctaa gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca
    28741 ttggctgttc cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat
    28801 acgatttttg aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca
    28861 ggcggacgtt atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga
    28921 tttgcaatgg gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct
    28981 gcatttaatg agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca
    29041 ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac
    29101 cgtaaagcaa aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg
    29161 atcggtgaag atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa
    29221 gaaaaaagtt ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg
    29281 actttattcg agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag
    29341 tttccgaaca acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag
    29401 accttggggg agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct
    29461 ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg
    29521 tgatcgaagt gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg
    29581 ccactggtga attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc
    29641 cgccatttca aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt
    29701 atttggatct gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa
    29761 aagcgattcg tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag
    29821 ggaaatctac accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac
    29881 atttctatgc gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat
    29941 tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc
    30001 agcctgaatt tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa
    30061 cttacacaga gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat
    30121 tgccatttcc tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg
    30181 atacacgttt tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca
    30241 aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg
    30301 ctgcagatcg ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg
    30361 gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta
    30421 aattcatggg agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact
    30481 tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga
    30541 cacgtttagg aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa
    30601 ctgattggcc acaatttgaa ttttcagaag acgaaggtcg atatgtctct gctcaccatc
    30661 cattcacgat gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct
    30721 atgcagaagc ttatgatatc gtcttgaacg gttatgaact aggcggaggt tctatccgta
    30781 ttcatactcg tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa
    30841 tggaagaaca atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg
    30901 gtattgcatt aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg
    30961 aagtcattgc ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta
    31021 tggtttcgcc tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc
    31081 cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg
    31141 aaaaacactt tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat
    31201 tcgaaaattg ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt
    31261 tgatcgcatc agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta
    31321 ttactggtct agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac
    31381 cggtatcaac aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa
    31441 tcgggaagaa gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc
    31501 atattttacc ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg
    31561 ctgtgatggg actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag
    31621 atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct
    31681 acttcaatgc attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt
    31741 acagtacgtt gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa
    31801 agaagatgca ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa
    31861 actgtatgcg tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata
    31921 agcagacagt attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa
    31981 tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc
    32041 gtttctatga ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag
    32101 acagacgttc gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg
    32161 cttttttatt tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat
    32221 gggatgatag aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat
    32281 acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa
    32341 aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg
    32401 gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt
    32461 ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa
    32521 caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg
    32581 gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg
    32641 ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa
    32701 atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc
    32761 tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg
    32821 atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc
    32881 tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac
    32941 ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca
    33001 agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga
    33061 ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc
    33121 atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca
    33181 gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc
    33241 aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag
    33301 gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta
    33361 atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt
    33421 cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca
    33481 tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg
    33541 aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa
    33601 ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc
    33661 cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg
    33721 gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa
    33781 gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa
    33841 ttaaaggaac aagatttgtt gaaagacact gttctgatcg gactaggaac gaatggctca
    33901 ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg
    33961 gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag
    34021 atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat
    34081 agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt
    34141 cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac
    34201 gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt
    34261 ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt
    34321 gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt
    34381 gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt
    34441 tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga
    34501 tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt
    34561 tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt
    34621 gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa
    34681 caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat
    34741 tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt
    34801 aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt
    34861 atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag
    34921 ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga
    34981 taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc
    35041 gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc
    35101 ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat
    35161 cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga
    35221 aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc
    35281 gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat
    35341 ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc
    35401 ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac
    35461 tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg
    35521 agaattacat actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag
    35581 ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc
    35641 tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa
    35701 gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc
    35761 ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt
    35821 ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca
    35881 agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa
    35941 cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg
    36001 acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta
    36061 tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg
    36121 agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt
    36181 gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat
    36241 gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat
    36301 tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga
    36361 ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga
    36421 attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat
    36481 atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc
    36541 gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt
    36601 acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga
    36661 caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt
    36721 tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc
    36781 tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac
    36841 accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa
    36901 tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg
    36961 cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct
    37021 gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca
    37081 gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa
    37141 tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg
    37201 tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt
    37261 agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg
    37321 gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa
    37381 attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac
    37441 agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga
    37501 tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat
    37561 gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca
    37621 gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa
    37681 cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga
    37741 aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct
    37801 cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt
    37861 ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt
    37921 ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca
    37981 tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc
    38041 agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa
    38101 acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga
    38161 aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat
    38221 aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat
    38281 tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa
    38341 agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac
    38401 tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa
    38461 aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc
    38521 cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt
    38581 gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca
    38641 agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa
    38701 atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct
    38761 cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct
    38821 attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat
    38881 tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga
    38941 acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga
    39001 acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag
    39061 tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc
    39121 aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt
    39181 tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga
    39241 tagacgaaaa agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc
    39301 agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca
    39361 gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag
    39421 taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca
    39481 actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac
    39541 acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca
    39601 agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg
    39661 tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca
    39721 tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga
    39781 gacattccca gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga
    39841 aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta
    39901 cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt
    39961 acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta
    40021 tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt
    40081 aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac
    40141 cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct
    40201 gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat
    40261 ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc
    40321 attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg
    40381 aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat
    40441 aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg
    40501 gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata
    40561 cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc
    40621 agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga
    40681 ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga
    40741 actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga
    40801 accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac
    40861 acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga
    40921 gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt
    40981 ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac
    41041 agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt
    41101 tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt
    41161 attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc
    41221 agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca
    41281 gataaatgaa caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt
    41341 caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt
    41401 ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca
    41461 acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt
    41521 gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat
    41581 cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt
    41641 tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg
    41701 cgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt
    41761 gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca
    41821 gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt
    41881 ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg
    41941 agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc
    42001 atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct
    42061 gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt
    42121 tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg
    42181 tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt
    42241 ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca
    42301 tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca
    42361 aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc
    42421 cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga
    42481 agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca
    42541 ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa
    42601 agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt
    42661 aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc
    42721 acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc
    42781 tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt
    42841 gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc
    42901 agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt
    42961 gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa
    43021 aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga
    43081 agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg
    43141 ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt
    43201 aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt
    43261 tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta
    43321 tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg
    43381 cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga atctcacggg
    43441 agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc
    43501 tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga
    43561 aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa
    43621 agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat
    43681 tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag
    43741 aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat
    43801 tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca
    43861 aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt
    43921 ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt
    43981 tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc
    44041 atatgaaatt ctttctcagc tagctgaaaa atctcttttt ctggtttttg gaaacctgtt
    44101 gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt
    44161 ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc
    44221 ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc
    44281 tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct
    44341 tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt
    44401 acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct
    44461 gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca
    44521 tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg
    44581 ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag
    44641 ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat
    44701 atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa
    44761 tcattatggt agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga
    44821 tttatttttt taatacagtc tcaatataac tattcaaaca taaaataacg ttatttgatt
    44881 tatttgttta tagtaaattt tatttagtaa tcattgcttt aaaatactac gcttatataa
    44941 cagagaaaat aagcgttata ataaagaaca g
//
LOCUS       DAYYCQ010000014        44888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000014 DAYYCQ010000000
VERSION     DAYYCQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.014"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="ACHAYF_000802"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="ACHAYF_000802"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8578262.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="ACHAYF_000803"
     CDS             1013..2260
                     /locus_tag="ACHAYF_000803"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8578263.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="ACHAYF_000804"
     CDS             2336..2680
                     /locus_tag="ACHAYF_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578264.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="ACHAYF_000805"
     CDS             complement(2797..3123)
                     /locus_tag="ACHAYF_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578265.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3738..4940)
                     /locus_tag="ACHAYF_000806"
     CDS             complement(3738..4940)
                     /locus_tag="ACHAYF_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8578266.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5057..5572
                     /locus_tag="ACHAYF_000807"
     CDS             5057..5572
                     /locus_tag="ACHAYF_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8578267.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5771..6217)
                     /locus_tag="ACHAYF_000808"
     CDS             complement(5771..6217)
                     /locus_tag="ACHAYF_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8578268.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6264..7742)
                     /gene="msr(C)"
                     /locus_tag="ACHAYF_000809"
     CDS             complement(6264..7742)
                     /gene="msr(C)"
                     /locus_tag="ACHAYF_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8578269.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8096..8725)
                     /locus_tag="ACHAYF_000810"
     CDS             complement(8096..8725)
                     /locus_tag="ACHAYF_000810"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8578270.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8709..9173)
                     /locus_tag="ACHAYF_000811"
     CDS             complement(8709..9173)
                     /locus_tag="ACHAYF_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8578271.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9660..10928)
                     /locus_tag="ACHAYF_000812"
     CDS             complement(9660..10928)
                     /locus_tag="ACHAYF_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8578272.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11159..11896)
                     /locus_tag="ACHAYF_000813"
     CDS             complement(11159..11896)
                     /locus_tag="ACHAYF_000813"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8578273.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11963..12532)
                     /locus_tag="ACHAYF_000814"
     CDS             complement(11963..12532)
                     /locus_tag="ACHAYF_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8578274.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12660..13532
                     /locus_tag="ACHAYF_000815"
     CDS             12660..13532
                     /locus_tag="ACHAYF_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8578275.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13874..14746
                     /locus_tag="ACHAYF_000816"
     CDS             13874..14746
                     /locus_tag="ACHAYF_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8578276.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14743..16701
                     /gene="nagE"
                     /locus_tag="ACHAYF_000817"
     CDS             14743..16701
                     /gene="nagE"
                     /locus_tag="ACHAYF_000817"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8578277.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17033..17884)
                     /locus_tag="ACHAYF_000818"
     CDS             complement(17033..17884)
                     /locus_tag="ACHAYF_000818"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8578278.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18007..18474
                     /locus_tag="ACHAYF_000819"
     CDS             18007..18474
                     /locus_tag="ACHAYF_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8578279.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18737..19219)
                     /locus_tag="ACHAYF_000820"
     CDS             complement(18737..19219)
                     /locus_tag="ACHAYF_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8578280.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19319..20182)
                     /locus_tag="ACHAYF_000821"
     CDS             complement(19319..20182)
                     /locus_tag="ACHAYF_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8578281.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20780..21655
                     /locus_tag="ACHAYF_000822"
     CDS             20780..21655
                     /locus_tag="ACHAYF_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8578282.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(21800..21952)
                     /locus_tag="ACHAYF_000823"
                     /pseudo
     CDS             complement(21800..21952)
                     /locus_tag="ACHAYF_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(22220..23551)
                     /locus_tag="ACHAYF_000824"
     CDS             complement(22220..23551)
                     /locus_tag="ACHAYF_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8578283.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(23700..24413)
                     /locus_tag="ACHAYF_000825"
     CDS             complement(23700..24413)
                     /locus_tag="ACHAYF_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8578284.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            24588..26012
                     /locus_tag="ACHAYF_000826"
     CDS             24588..26012
                     /locus_tag="ACHAYF_000826"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8578285.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            26311..27018
                     /locus_tag="ACHAYF_000827"
     CDS             26311..27018
                     /locus_tag="ACHAYF_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8578286.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            27005..27328
                     /locus_tag="ACHAYF_000828"
     CDS             27005..27328
                     /locus_tag="ACHAYF_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8578287.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(27417..28454)
                     /locus_tag="ACHAYF_000829"
     CDS             complement(27417..28454)
                     /locus_tag="ACHAYF_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8578288.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(28775..29758)
                     /locus_tag="ACHAYF_000830"
     CDS             complement(28775..29758)
                     /locus_tag="ACHAYF_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8578289.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(30173..30943)
                     /locus_tag="ACHAYF_000831"
     CDS             complement(30173..30943)
                     /locus_tag="ACHAYF_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578290.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(30936..31871)
                     /locus_tag="ACHAYF_000832"
     CDS             complement(30936..31871)
                     /locus_tag="ACHAYF_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578291.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(32187..33836)
                     /locus_tag="ACHAYF_000833"
     CDS             complement(32187..33836)
                     /locus_tag="ACHAYF_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8578292.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(33953..36556)
                     /locus_tag="ACHAYF_000834"
     CDS             complement(33953..36556)
                     /locus_tag="ACHAYF_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8578293.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(36568..37617)
                     /locus_tag="ACHAYF_000835"
     CDS             complement(36568..37617)
                     /locus_tag="ACHAYF_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8578294.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(37643..37957)
                     /locus_tag="ACHAYF_000836"
     CDS             complement(37643..37957)
                     /locus_tag="ACHAYF_000836"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8578295.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(37973..38419)
                     /locus_tag="ACHAYF_000837"
     CDS             complement(37973..38419)
                     /locus_tag="ACHAYF_000837"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8578296.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(38420..40342)
                     /locus_tag="ACHAYF_000838"
     CDS             complement(38420..40342)
                     /locus_tag="ACHAYF_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8578297.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(40554..41450)
                     /locus_tag="ACHAYF_000839"
     CDS             complement(40554..41450)
                     /locus_tag="ACHAYF_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8578298.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(41443..42123)
                     /locus_tag="ACHAYF_000840"
     CDS             complement(41443..42123)
                     /locus_tag="ACHAYF_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8578299.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(42124..42906)
                     /locus_tag="ACHAYF_000841"
     CDS             complement(42124..42906)
                     /locus_tag="ACHAYF_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8578300.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(42899..43816)
                     /locus_tag="ACHAYF_000842"
     CDS             complement(42899..43816)
                     /locus_tag="ACHAYF_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8578301.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(43980..44234)
                     /locus_tag="ACHAYF_000843"
     CDS             complement(43980..44234)
                     /locus_tag="ACHAYF_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578302.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT        13575 a         9154 c         7719 g        14440 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa aaacggtaaa
     3421 atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac gctgatttta
     3481 caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     3541 aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac gctgatttta
     3601 caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
     3661 aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt
     3721 tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg gtgataaatg
     3781 atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata gctttcacca
     3841 atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca
     3901 aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat agcaatcgtc
     3961 gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct gatcggtaca
     4021 aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa taagacaaat
     4081 gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc
     4141 gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc taattcagaa
     4201 tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa attatataac
     4261 aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac accttcccaa
     4321 aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa
     4381 ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc actgcccaag
     4441 accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc atacatcatg
     4501 attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc agtagagaca
     4561 aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc gatattccca
     4621 gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat caatgttttt
     4681 ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc agtgactaac
     4741 atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg tgcagcaatc
     4801 gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca ggtagctgct
     4861 acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg tatatctatt
     4921 tgattctcca atatttccat ccctttctct ctattagcat catacatgat ttattttaca
     4981 tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag aaaataagac
     5041 gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc ttttatccaa
     5101 accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag cccatcacag
     5161 tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg ttttggacaa
     5221 agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg ctcttgtaga
     5281 ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc tagcaaaaat
     5341 ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca ctagagaaca
     5401 acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct cgctagacaa
     5461 gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt gtcagcagga
     5521 actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt aagggataaa
     5581 cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa gagcaataca
     5641 gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg atcactattt
     5701 ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt tttcaaataa
     5761 cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa agcgggccaa
     5821 cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca ttcatataag
     5881 cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa gacatacatg
     5941 tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc ttgctttctg
     6001 gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg aagacatcca
     6061 aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat aaatgaagat
     6121 cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa cacatcattg
     6181 ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc tttaaatttg
     6241 tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt tattttctgc
     6301 ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc atgagaagta
     6361 aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat cgttgccata
     6421 tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt aaaaagcaaa
     6481 gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt cgtcaaagtt
     6541 tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga aaactctgtt
     6601 tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc cttttgctga
     6661 tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt gcggatatgt
     6721 tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt tttacctaaa
     6781 gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa ttggacattt
     6841 tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg aaaacggatt
     6901 tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct ctctaatgtt
     6961 ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga agacaaccga
     7021 tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt ttccgcttgt
     7081 tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat cgactctgct
     7141 tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact ataattcccc
     7201 gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag ctcatctaga
     7261 aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag ctcttcaatc
     7321 agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag catcccttgt
     7381 tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc gctccatttc
     7441 cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa atttaggtcc
     7501 tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc tgtttggatt
     7561 ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga tttaccgaca
     7621 ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga atcaatagat
     7681 agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc taaattttcc
     7741 atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa aaagagcaga
     7801 aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga aataactatg
     7861 actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga aaaaatggac
     7921 atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta gttgttattc
     7981 aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt ttttattagg
     8041 ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa accttttatt
     8101 cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct tctaccgctt
     8161 ttcctattga taaaataata actggaacat aacggtcttt atccaaacca aatgcctctg
     8221 ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga tcacgcgcaa
     8281 ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg ttcatttctt
     8341 ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct ctgacttctt
     8401 ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat tcgtaacatt
     8461 gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc gtattgaaac
     8521 ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca aagcgccatg
     8581 gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt
     8641 cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca gtcataattg
     8701 ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt tgttatattg
     8761 caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc tacatcgctg
     8821 aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg cttgattttc
     8881 ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg atttctctga
     8941 cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc tccttgatca
     9001 atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt cagtctcatc
     9061 atgatctcgt atctagtaag acttataccg atattttctt caaagatctg gacgattttt
     9121 tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga cataatttcc
     9181 tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata aaaaaagaaa
     9241 aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag tatagagaaa
     9301 agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc taaaaccaag
     9361 attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa ttttacaccg
     9421 ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa attggatcag
     9481 tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt ttttgagtaa
     9541 acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa acatgaaaat
     9601 ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac tacctctttt
     9661 taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag ccaatctctt
     9721 tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt aaatagtcga
     9781 taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca accgattttt
     9841 gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact gtaaggattg
     9901 tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc ttcatattcc
     9961 cagcctacag ttggcagata atttttttct ccggcattca atgtatataa atgttctcct
    10021 gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt atcatactgt
    10081 gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg cgcagtggca
    10141 taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct tttccatgca
    10201 ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt tacgttatct
    10261 tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt aatccattgc
    10321 ccattcacat attccgaagt aggcatcgta acagattggc cattatagct tcctttaatc
    10381 ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc actttcaacg
    10441 atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa actttgtgca
    10501 gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg agcataaaaa
    10561 gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg tgtcaatgtt
    10621 tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt ctgagcttct
    10681 gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg ttcttcggtt
    10741 gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc gttttcaatt
    10801 cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc ttcagctagc
    10861 actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa taaattcttt
    10921 gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag tgtaactaaa
    10981 aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt aataaatttg
    11041 ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg ccttaaattg
    11101 atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc gttatggatt
    11161 atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg tctggcgttt
    11221 ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat tctttttgcg
    11281 cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat tcagtttgaa
    11341 cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct tgacgtaatc
    11401 cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa tcaggataaa
    11461 caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt ttttccatga
    11521 ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca agcatatttt
    11581 gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct gcattattgt
    11641 acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct tggacttgtg
    11701 caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa gattctttta
    11761 ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct ttttctgcta
    11821 atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca atcacttttt
    11881 ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct tatactcact
    11941 atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg attttggcag
    12001 ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca ttcgctgcaa
    12061 caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct ccgatccaag
    12121 catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt ggatcggtta
    12181 gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct tctaaacaga
    12241 tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc cctatactaa
    12301 tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca tctactttgt
    12361 cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac ccagtattca
    12421 gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca aagagcggat
    12481 ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc attttgctcc
    12541 ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta taaaacatac
    12601 ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg agggaaaaaa
    12661 tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat atcagccgag
    12721 cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa gatttagaag
    12781 aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg acggattctg
    12841 gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca
    12901 atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc gaaactaaag
    12961 caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct gatatccagt
    13021 ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc ggattacttg
    13081 attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt cgactgccag
    13141 cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag caaacagtca
    13201 tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg tcagtggaca
    13261 gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc ggaacatata
    13321 acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca ttagctctag
    13381 atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat ccgccattac
    13441 aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct gctcgtaaat
    13501 ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc gctttcagct
    13561 ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa gtttcttaat
    13621 ttgttccttc cttttatttc tgataattcc agtaaattac tataattata aaaaaagaat
    13681 tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg tttcatgtga
    13741 ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg aggtttttcg
    13801 tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa
    13861 aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat tggtagatga
    13921 cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa aaagaaatga
    13981 tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa
    14041 gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag aaacgatcat
    14101 tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga tcgctttgac
    14161 cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc gcaacaaact
    14221 tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat gggcagtaga
    14281 gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat atatcgcaat
    14341 ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc gtgaagtgac
    14401 gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata tccattcaga
    14461 accattttca ctgaattatt caagactggt gaatcattta cgactgcttc tacaacgaac
    14521 ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca aacgaaaata
    14581 cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct
    14641 cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt taagaagcgc
    14701 aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc atatctacaa
    14761 cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc tttactagta
    14821 gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt tttacaagct
    14881 ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt agcactaggg
    14941 atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca
    15001 gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc agaatcagtt
    15061 gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg attgattgcg
    15121 gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc tttctttagc
    15181 ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta
    15241 ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt cattttgagt
    15301 ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat ccctacagga
    15361 ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa tgatattggt
    15421 aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc
    15481 ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa tgcacgtcca
    15541 gaaaacaaag caaaaacagc ttccttgatg ttagcagcag cttttgcttc attctttact
    15601 ggtgtaacag aaccacttga attctcattt atgttcgttg cttggccatt atatgtcttg
    15661 catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg gacagcagga
    15721 tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat
    15781 cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta ctttggcttt
    15841 aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc agatctttca
    15901 gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc aagcaaaatc
    15961 tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac tacacgcctt
    16021 cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc cactggcgta
    16081 cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa
    16141 ttcgttgcag atgagatggc aaaactacat ggtggtgctg caacacgacc tgcacaaaca
    16201 aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga tatttacgct
    16261 atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt cgcggaaaaa
    16321 atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc tccaatcgct
    16381 ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac agatgctggt
    16441 atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga accattcact
    16501 ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat tgattgcttt agttgatctt
    16561 gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac aaatggcgac
    16621 aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc
    16681 tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg ataaacaaac
    16741 aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg gcgttctcct
    16801 gccaactccg cggtaataag tccagccaaa cgtaacatat gaagagctat tttttatttg
    16861 actcttctta ttacttgatg cagaacagct tttttacaac ctctgataaa cggtgttcaa
    16921 aaaccagttt atcggtatga agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
    16981 aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata cattattcct
    17041 gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt
    17101 ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa
    17161 acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat
    17221 cacttgcttc aataatttct aactttttac ctgtcgcacg ctctaatgcc ttggctaatt
    17281 cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta
    17341 gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt tttccatttc
    17401 ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct
    17461 ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt
    17521 cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct gcaaaacttg
    17581 tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg
    17641 cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt
    17701 ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca
    17761 gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct
    17821 tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat
    17881 gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta catttataaa
    17941 ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga
    18001 caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa
    18061 atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat
    18121 gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa
    18181 accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc
    18241 tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat
    18301 tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa
    18361 aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt
    18421 ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct
    18481 ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg
    18541 gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc
    18601 atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc
    18661 aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt
    18721 cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt
    18781 tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt
    18841 ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag
    18901 gcgattctgc taaagcgatt acaacaagat acgttccttt tgggaatggg atgattcggg
    18961 tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat
    19021 agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg
    19081 ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat
    19141 ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt
    19201 cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa
    19261 aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta ttttttaact
    19321 atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc
    19381 attttgtaaa agagatctcg ggagcttcga ctaactcata atctgaagaa ggaagccatt
    19441 ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa
    19501 aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt
    19561 ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag cgggctccat
    19621 caaatccata agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct
    19681 cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt
    19741 caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga
    19801 tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag
    19861 atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa tcacgaaaag
    19921 ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg
    19981 atccatctag gagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta
    20041 gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat
    20101 tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca
    20161 attggttcag atctttaagc atatttatct tcctccttgc ttgccttttt atcatattat
    20221 caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg
    20281 tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc
    20341 tatctatgtt ttacctttag aaaaataaat tttggggaag acacattcta agtaaacaaa
    20401 gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa
    20461 attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga tacatcatac
    20521 ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt tttgtcagat
    20581 aaataaaaaa caaaaaaagt tccatcttta aaacacgcta ttcttttact ttttctcaca
    20641 ttctctaaga aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag tgattatcaa
    20701 atatgtaaaa tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt
    20761 ttcatgaaag gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga
    20821 acgcggttac agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta
    20881 cttgtccgca acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa
    20941 gaccaacatg acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc
    21001 tgctgtctta ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga
    21061 actctcgtcc cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat
    21121 tcttatagaa catccgcaat ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat
    21181 aaattattca gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa
    21241 caaaaacagg aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt
    21301 tgaatcgctc attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat
    21361 tgctactctc gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga
    21421 ggactgttat tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca
    21481 aatgttctct tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta
    21541 ctccataaaa atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa
    21601 aatagataaa aaaactaaag aaacggtaaa gaaagacgat ttcaatgaaa aatagcaagt
    21661 aaacgataac aaccatccta aatccacaag aaatacagca gaccttgctt taaaaacacg
    21721 aatcatgcaa aggatgaagt aaagaagact taacgcgtac ccaatattag tcatttgact
    21781 attaggtacg cgttttgttt tataagtcat gacaaatttc cttcgttgct gggtagattt
    21841 tccggcattg ttgatagatt ttatgatact tttctacatt ttgtaagaga ggttccactg
    21901 catctgtata ttggataaaa ttctgacaac aactttcagg tgtgtcgaac cactctaatc
    21961 tttaaagata aatattaaaa taacgattaa taatattaac agtctttatt tttcaccacc
    22021 aaaaacctat ctttgcatat actaaataga cgtatataca cagataggtt tgtcttgtga
    22081 aaaataatga ttatccttac caaaaaatgt ttgtttttct acatgttcgt acttatcaaa
    22141 aaatatgatc cttattgaaa ttactggcta tttatattcc aacgacttct tgtgttaatt
    22201 tttgttatct catagtaaat tatgtttcat tttcacttaa tttagttgtc acgtgttcgt
    22261 caattcgtaa aaacggtaaa tatataaaca cacttgcggc tataattaat atttgtaaaa
    22321 cagctgctcg ccagtctccg gctgttgcta aaaatcccga tataactggt ggcgtagtcc
    22381 aaggaatttg cactactaca tgattaatta atcccacagc agttgcaaaa tatgctactg
    22441 tcaaagaaaa aatcggtgca agcacaaagg gaataatcag actgatatta aatacaatag
    22501 gcaatccaaa aataataggt tcgcttatat tgaatatagc gggagttacc gataatttag
    22561 caaagtcctt ataatctttt ctacgactga agataaatat tgcaatcaat aaagcaatcg
    22621 tatttcccga tccacccatt accgcaaagg tatctttaaa ggccatattg ataatatgtg
    22681 gaatttgctc tttgtttgca taagctgcca tattttcttg catattctga atcaaaaacg
    22741 gatctaacaa tgaacctgat attactgctt gatggatacc aaatccaaaa aatagattag
    22801 caatagatgt aattaacaaa aatcctggta aaccagttgt aattttactt aatggctctg
    22861 taactatcgt ggtaattagc ttatttatat ccatattaaa caattgaatg attataaatg
    22921 acaataaagc aaacgaggta atcgtaataa tgattgggat taatacatta aaagacttta
    22981 aaacagcggg aggaatattt ccggatagct gaatctgtag tttcttatta ttagaaagtt
    23041 taataaataa ttctgtagcc gctaatccaa caaatatacc tacaaacatt cctgaagctc
    23101 ctgtatagga tataggtatc acacccgtca cttcaacagc tttatccgca ttttcaggag
    23161 taaacatcat ctgcataggt gttaatacaa caaatgtcga aatagcaact aataccgctg
    23221 caatatgatt tgtataattt ctgcttgcac acatatgata agatactgct gctacaacta
    23281 ataatgtagt tatgcttaat gtaccattgg caattgaagt accaatttgt tgtaacgtgg
    23341 tcaatgtact agtatcgaca atttccccca tgaatcctgt tggttctaag atcactgaat
    23401 taataagtgt cacaaatcct gctaacatca taaatggcat aaatgtagca aacgcatctc
    23461 gcaaagttct taaatggatc tgattcccca acttagtcgc aactatacca aatttttcaa
    23521 atacaatgtc cttcctgcta cttttgccca tattttttct cctttatgct tattgtaatc
    23581 gattactttt tccacccaaa taatacatca cttatcaaaa aaagtaaata cttttaatta
    23641 aatatatata tcttttttag tataaaaaaa taagctctga aaaaataatt ttcccgagct
    23701 tatttttcaa caaaaacagc ttcaaaaaca aagtcttgat ataaatgtct cgataccgaa
    23761 tactcgaaag gtttaccaga cgatagagca acaactttgt ctatctcaat cataaattca
    23821 cctggatcta tttttaatat ttttgcgtca aattcattcg cagtatctcc tttgatacgt
    23881 atgtgcgaag tctttatttc cagacccagt tcatttgtga tataagcata tacagatttc
    23941 tttaaatgtt ttggttccaa gcctggtatg actgctaatg gcatgaatgt ttgctcaatc
    24001 gagtatggtt gacaatcaat aaaacggctt cgtacaatat tatatacata gtcttttaca
    24061 ctgatactta atttatcagc tacttttttc gatggaagtt caattgagaa agtatggatt
    24121 tctgaatcta cgtgttcttc accaaccacg ctaaacaaac cttcgtttgg accaagaact
    24181 cgtgagttat caattggtgt tctgacgact tcagtcccat acccacttct acttcgcaaa
    24241 actccttcgg aaacaagtaa gtctacgcct ttctttactg ttaacttgct tgtatgataa
    24301 ataactgcca attcatctcc tgtaggaagt ttctcaccaa ttttatactc tccatttaca
    24361 atttttttct ttatatctaa gtaaatttcc atatacttcg caattttttt caatggtgac
    24421 cctccaatca aaaacatatc ttcatctata ctttaacata aataaaccga taaaatccac
    24481 atcaaaatta tacacacaat aaaaaagata tatatattta aacaaaacta tttactttaa
    24541 gttgtaagcg gtgttatcat taagtggaaa caggaggtaa aaaaattatg acaactatga
    24601 aaaagctaac ttttccagat gatttttggt ggggttccgc ttggtcagct gagcaagcag
    24661 aaggaaaagg agaaacagga aaagcagaga ctatctggga tcgctggttt aaagaacaac
    24721 cttaccggtt ttatcaacga atcgattcct ctattacaac cgatcacatc catcaatata
    24781 aagaagatat agaactaatg aaaaaaactg ggcataactc ctttcgcgtt tctatttctt
    24841 gggcacgaat gtttccaaat ggcggtaccg gaaaagtaaa tcccaaagcc atttctttct
    24901 atcgaaaatt attttccgaa atgaataata acggaatcaa agtatttgcc aacttatatc
    24961 attttgacat gcctgctact cttcaagata aaggaggatg ggaatcacgt gaagtagttg
    25021 atgcttatgt tcattacgct gaaacttgtt ttaaagaatt tggtgactta gtttgtcatt
    25081 ggtttacctt caatgaacca cttgggccta ttttaggtac ttatcttgaa gattttcatt
    25141 atccaaacct cattgacttt aaacgcggag cgcaagcagc attcttcact atacttgcac
    25201 acgcaaaagc aatacaagta tttaaagaac tagctcttcc tagtaaaata ggcgttattc
    25261 taaatttgag tcctactttt cctcgtagtc aaaatcctgc tgatgtgaaa gcagcagaaa
    25321 tcgctgattt attttatacg cggagctttt tagatcctat ggttaaaggg aatttttcta
    25381 aaaagcttat cctgttatta caagaatata atcagcttcc tgaagattat acgaaagaag
    25441 atcttgtatg tatctacgaa aataccgctc aaattcttgg actaaactat tacgagccac
    25501 gtcgcgtcaa atctcgttct tctatggtgg atacatctag tccattttta ccagagtggt
    25561 ttttcgaacc acatatcatg cctggacgac gaatgaacga ataccgggga tgggaaattt
    25621 acgaaaaagg aatttatgat ttgtgcatgg atatcaagaa ccattatgga aacatcgaag
    25681 catttatctc ggagaacgga atgggcgttg cggatgaaga acgtttcatg gatgatacag
    25741 ggcaaataat tgatgattat cggattgagt atatcaaaga tcatcttgct tacttatgga
    25801 aggcaataaa tgaaggatgt aacattaagg ggtatcatct ttggacattt attgattgct
    25861 ggtcatggat caacgcctat aaaaaccgat atgggctggt ttcactcgac cttactactc
    25921 aaaaaagaac aattaaaaaa agtggcgaat tttacaagca attaagtgat acaaatggat
    25981 ttagatacga tgtagacttg ttagttgaat aatattctaa aattccactt gaagtgaaca
    26041 cattagaaaa caagaataat gagaaatacc aataacaaat actttttctt catacttcat
    26101 ttatacttgt cagtataaga gactgtgaca aatgtagtga tcctcagaag ttaggtctgg
    26161 aaatctaact tgagaagatt tttttaaact acgtccagtc ttttttattt gtttaaaatt
    26221 gaagttgaca agtcttcatt aaaaatttag aatattcaca agtttgacga caattatgaa
    26281 aatttatgtc gtgctaaagg agttttgtta ttggatgaaa agttagatat aaaaactgcg
    26341 atcaaagaca cgttacctac cgtttttggt tatatcggta ttggacttgc atttggtatc
    26401 gttgggaaag ctgccgggtt tcatccgctg gtcgtcacgt tgatgtccct gctgatctat
    26461 gctggttctg cccaatttat cacggtcagc atgcttgcta gccatagtcc actgctctcc
    26521 atcgttctct cgacctttct agtcaattcc cgaatgattc tgatgagtat gacgattgct
    26581 ccttatttca agaaaaatcg gctgcttcaa aatctgctga ttggcacatt gctgacagat
    26641 gaaagtttcg cactgggaat gaataaattg aactatactg gacaaaagtt aaactttcgt
    26701 tggatgaata cagctaattg gatttcttat ttgacgtggg tgggttcttc tcttgttggc
    26761 gcacttttag ggaattttat tactgatccg aaaaagtttg gtttggattt cgccatcgtc
    26821 gctatgttta tcgggttact ttatcttcaa gtcatttctg atagaaacac ctctaaacga
    26881 ttacagttga tcctgattgg tctcactttg attctggtat atgtcggact gatttttatc
    26941 ccaagtaact tagtgatttt agttgtaaca ctgattgggt gcggattggg ggtgtggatc
    27001 aaacatgcct tcttttgagt ttacattgct gactatttta ggttgtactt tagcaacttg
    27061 gctttctagg atcctaccat ttgtcttact gaaaaaattc gatttgccgc aaccgttgct
    27121 tgagtattta agttttgtcc cgatcgtcat tatgtctgct ttatggttca gcagtctatt
    27181 tacccaaaat atcggacatc tgccgcagat caatctcgaa aatgctttgg cttcagttcc
    27241 tacactttta gctgctattc tttcaaaaag cttattggtg atcgtcttgg taggaattct
    27301 ttccctttcg ttgattcgct tattataaat aaaaaaacaa gagcttatgg caaattactg
    27361 tcatgcgctc ttgtttttat caaagtcaaa taagaaaaaa atcggcgtat tcttgcttat
    27421 ccgttgaacg gaccactaac aacgactttt cctaatatat gtcctgtttc tacgtctttg
    27481 gttgcttgtt tcaagttctt cgcattcatc ccatctgaat agaccttgct caatgtactg
    27541 cggataatcc cttcatttgc taaagcagca atgtgcgcta aagcttctcc ttgcgtttcg
    27601 atttcatagt tatagtctgt tttagcgaac atatactccc agtcgaagct gatagattgg
    27661 tttttccaaa cacctatatc taacggctct ttgatcccta cgatcgttcc cacatgtcca
    27721 aatggtcgga caaggtgttt gatttgatca aagtagcggg taatatcgaa taatacagcg
    27781 acatactcta cttgatcgat tcctaattca cgaaaacttt tacttaagtc gtgatgataa
    27841 tcgattggat agtctacacc tgtttttttc agccattcaa aattcttcgg acttgcagtc
    27901 gcgtagactt ctaggccagc ccaatgcgct agttgattca aaatcgaacc gacgccccca
    27961 gctccgttga tcactaagat ttttttcccg cggttggcat tttcttctgg agtcagacca
    28021 aatttttcaa ataacaattc ataagcggtc aatgaggtca acggcaaggc tgcggcttct
    28081 tcatctgaaa gattttttgg tgcaagagcg acaatacgtt cgtcaaccag ttggtattct
    28141 tgattgcttc ctgctcttgt cgttgtacct gcgtaaaaaa cgcgatcgcc gacagaaaat
    28201 ttttgcactt catcacctac agcaacaact ttcccgactc catcaaaccc taagatacgt
    28261 aaagcatttc ttatcccgtt atcttggcga agtttcgtat cgacaggatt aacagatact
    28321 gcgttgactt ttaccagaag atcccgtgaa ctgggtacag gtatctgttc tttttcatca
    28381 ataaagcttt ttggatcatc gatcgacaac ccttcataaa aaccgactac cttcattaat
    28441 tccttttctg acatacgaat tcctccttag cttcatcaag ttggagcatc catacagttt
    28501 tcatttcaat atatatagta tgctatttgt tccactttta ttcatcataa ttattctctt
    28561 acttaaagta aactatttgc acttttttgg ttaaaaaaga ggttgcgaca agacttttgt
    28621 cacaacctct tttaaacggt gttcaatcaa ctgaccctcg gtattcgctc aaaaaacgac
    28681 aaaatatgag gagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt
    28741 cacgacctct aattcacggt gttcaagcaa gaaatcattg ttcactgaaa agcgttggat
    28801 gttctttttc aaaggtgtct aagatctctc gtacttcatc agtagatttc gtattcatca
    28861 actgaactcg caagtcattt gctcctggaa atcctttgat atagatttta aagaaacgat
    28921 gtagtcctgt gatggaacgc gggatgattt ctgaataatg atcttgcaga tctagttgca
    28981 gacgaagtag tccgatcagt tctttttctg tatgttccct cggttctttt tcaaatgcat
    29041 aaggattctt gaaaatcccc cggccaatca tgatgccatc tacgccatat ttttcggcca
    29101 attcttctcc tgtctggcga tctgggatat ctccgttgat cgtgatcagt gtttgcggag
    29161 cgatttggtc acgtaaagca atgattttag ggatgacttc ccaatgtgca tctactttac
    29221 tcatctcttt acgcgtacgc aaatggacag aaagattggc tatatcttga tgaagtaggt
    29281 gactgatcca ttcttccatc tcagacattt ccgtatagcc gatacgcgtc ttgacgctga
    29341 caggcaaacc gccagcttta gccgcttcaa tcaattgtgc cgctacttct gggcgaagga
    29401 tcaagccact gccttttcca cgttctgcta cattagggac tgggcagccc atgttgatat
    29461 cgattccttg gaagcccatt tctgccatag caatgctcat ttctctaaaa aattctggct
    29521 tgtctcccca aatatgggca accatcggct gttcatcttc tgtaaaggtc aatcttcctc
    29581 gtacactgtc ttttccatct ggatgacaga aactgtctga gtttgtaaac tcagtaaaga
    29641 agacatccgg tgctgctgca tgctttacga catggcgaaa tacaacatct gttacatctt
    29701 ccattggtgc taaaacaaaa aaaggctttg gcagctcttt ccaaaaatta gtagtcattt
    29761 atttgttact ctccattcta tcaagcagtg aagtcgcaat ctttctcaaa aacattgtca
    29821 ttatactagg aaacaaaaaa agaagcaaag tgaacagctt aaaagatgtt tctgattgag
    29881 caaaaaaaga ctgtgacaaa aatcttatca caattccttt tccggaataa atggtgttca
    29941 aaagcttgca tctgcggtaa taagtccaac caaacgaaaa ataggaggag ctatttttgt
    30001 ttggctgttc ttattacttg atgcagaaca gctttttcac aacctctcca ctaaacggtg
    30061 ttcaatcagc tgaccctcgg tattagctca acaaacgaca aaatatgagg aactattttc
    30121 ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt ttctaacttc
    30181 tcaatcctct tcctttattg atcaaatacc aagtaacggc gtacaaaaca acgatgaata
    30241 gcacaagtac gatcatcgat gttgctactg gcacatcgat tgttccaagg aatccgtaac
    30301 ggaaaccgga gatcatataa acgatcggat tgactttcga aacagcttgc caaaatggcg
    30361 gcaacataga gatggcatag aaaacgccac ctagataagt taacggctgc aagacaaatg
    30421 taggcacgat cgatacatcg tcataagact gagcaaaaat cccgttgagc agtcctgcta
    30481 acgaaaacaa gattgccgtc atcaataatg taatgatgac catgaaccat gaatacacat
    30541 gaagtggtac aaagaacaag gaaatgatcg ttacgagaga gccgacgaga atactgcgac
    30601 ccactccgcc aatcacaaag ccccaaatga tcacatgcgt aggaacaggc gctaccaaga
    30661 tttcttcgat gtttttctgg aatttttgag agaagaagga cgaagaaacg ttggcatacg
    30721 aactagtgat agcagacatc ataatcaaac caggcacgat gaattccata tatgaaaaac
    30781 cacccatgtc accgatccga ccaccgatca tttttccgaa aataacaaag tacaaggatg
    30841 tcgtgatcac aggcggaacc agtgtttgga cccagatacg caaatagcgg ttcgtttcct
    30901 tgatagctaa actttttaat gcagtaaaat aaagattaaa catttttctc ctccgtatga
    30961 ttattttctt ctgtgatttt caaaaacagt tcttctaaac gattcgactt attcctcata
    31021 gaaagaactt tgattccttg tcttgtcagc tgatcgaaga ttccgttcac cccttgattt
    31081 cgttcaactt cgacagcgat tgtctgttca tcttcaaatg tacaagcgta tccttcaatc
    31141 acaggtttgg tctcatatgg cgctaaatca aagataaatg tttcaaattg cagtttggat
    31201 agaagctctt tcatactagt attctcgatc agttcacctg attggatgat cccgatgttg
    31261 cgacaaagca tttcagcttc ttctaaataa tgcgtcgtca aaatgatcgt tgtaccattt
    31321 tcgttcaatt ctcggagaaa ctcccacatc tcgcgtctca attcaatatc gactcctgct
    31381 gttggttcat ccaagatcag caaacgtggt tcatgcatca atgccctagc aatcatcagt
    31441 cttcgcttca ttcccccgga aagcatacgt gcacggacat ttctcttttc ccaaagattg
    31501 gactgcttca aatatttttc gctgcgtttc atcgcttctt tgcgagagac accgtagtag
    31561 cctgcttgat tcacgacgat ttgctggacc gtttcaaagg gattgaaatt gaactcttgc
    31621 gggactaatc caatctgttg ctttgctcgg actagatccg tatccagatc ataaccgaag
    31681 acattcactt ttccagatgt tttattgact aaagaagtaa tgattccgat cgttgtagac
    31741 ttcccagctc catttggtcc taatagcgca tagaaatctc cttctttcac ggaaagatcg
    31801 attcctcgca atgcttcgac cccacttcca taaacctttt tcaaattatt tatttcaagc
    31861 gcatttgcca tttatttcct atctccttat agacatgatt ctttattgta tcatggaata
    31921 cagcagatga aaaaacaact tggttccagc gaaaaatcag gttgctgggc tcaggaaaat
    31981 ctcctaatga aagacatcaa tcggacgaag tcagacaacc ttttcagaaa aaacaaatcg
    32041 aagataaagg attactattc atatgtgcaa caaaaggagc gtgagacaaa agtaaagaac
    32101 acttttgtct cacgctctaa acacgtataa acggcggaag caaaagccaa gatttttgat
    32161 ccaataaata ctttcgatca cttcgcctat cccaatacat gccactgccg cttttcatca
    32221 aataacgcgg cttccgtttt tgcggtttct actaaagtct ctgggtaatc caaaactttc
    32281 agcaaggcaa tcgcatttcg ggtccttgaa ggtccttctt ttaatttgaa atcaaaactg
    32341 attccttttc cttctgttac ttgttcttca aagtggacat tctcacaata attctttaaa
    32401 atctctgtca attcgatatc atgtgtggcg acaaatgcta aagatggata ctccgcaagc
    32461 caccgaacga ctgaagcgga agaagcaatc cgttcgatcg tatttgtccc ttttaaaatc
    32521 tcatctacga agcagtagca gcgttctttg gaagccactt ggtccaacaa acgtttgatc
    32581 gatttgattt ctgaaacaaa ataactgtcg ccttcaaata aatcatcttc gattgccatg
    32641 gaagtcaaca catgtccagg ttgcagggta aactgttctg caatcgctgt ttggatcgtc
    32701 tgtgctagaa tacagctgat ggctatactt ttgacatacg tagattttcc tgatgcattg
    32761 gaacccgtga ctaatgtatt cttctgccag ttgaccggat tcactaccgc ttcttttaac
    32821 aatggatgat agatatcttt cgctgtcaca ccgccctcag taaactctgg tcggcaagtg
    32881 atgggcatat aagtacggta attcaatatt gcagcggcta cttctaattc tcccagcaat
    32941 tcccacagct tgatggcttc agttgaatgc ttttctaatt tagtgagaac gaaatgatag
    33001 gagataaatg gcagcatgaa catgatgttg acatattcaa aaatcaactc tgcttcactc
    33061 ccactcttca tacgaaatga aaaacctaat ttagggatgt ctcttaatgg tttagtttgc
    33121 tgtttcagct cttcttgaag tggtgtcggc agttttgcta gctgattggc ggctgaaatc
    33181 gttgaaacca aataactcat gctattcagc tctgtttcta gtgtttgttt cttcactaga
    33241 taatagaccg tattgaatac taacccaccg attgccaaat agattcctag tacttgtcct
    33301 aaaagaagca aacccaaacc caccagcgga agcaagccaa gaaaaataaa taaacctacg
    33361 cgtccaatct ttttactttt tccatccgct agatattgtt ttgcaaaatt atgatctttt
    33421 tttcctaaac gagcgaattg atattggatt ttttctctca gttgaggatt ttcctgataa
    33481 aaagcaatca gtttttctaa ctgctgatct tcaccaaaat caaagctgcg taaacgttga
    33541 tacaacgctt ctgaacctac actagaatag gtactgttga ttccttcgaa tacttcgaac
    33601 atatccaggt cataccaagt aagatcatcg atttcgctat cccatgaacg gaacttcttc
    33661 tccgtgagcc atgcttcctt caagctttct tctttatcaa atctcggttg ataaggaatt
    33721 ttttcccatt tgctacgaac tgtttccttt aatttcaaac gtgaaaaaat gtctaaagca
    33781 actaataaaa taacagctgc aataataatt aaaacgataa attctctttg acccatatcc
    33841 ctctccgttc ctttatttgt tagctttagt atagcactgt tgcgctattt gaaaagaggg
    33901 taaaaaaaca cctttcaaaa taaaaggtgt ttttttcttg tatattacat atttatctct
    33961 catttatcaa aattgacaaa taatcatagg gaggaattgt tgttaattct tgtttattct
    34021 ccagtgcatt gacaactgtc ccaagggaaa ggatagcctc aggtactatt ttttcttgtt
    34081 ctgttggatt agctaagcga agaacaaatc cttgttcatg aaaataagaa ggataaatcg
    34141 atgatacaat taagtcttca ggtaattcaa tcaatgaaaa tttacgagga acatcaagtt
    34201 cattttgtaa tggccaaatc ttgttatcta acctgtgaat aaatacattc agtttctgct
    34261 tttgataaga aatagattgt tctaaccgtt tttctgcctg ttttgccact ttgaactcat
    34321 taaatcttcc ttcttctaca ttaaatccaa aagtgacttt atactcacct atctcttgag
    34381 ctagcggtgt ttccatcatg acatgccctt catttgtcgt atctcctgaa gctcgcccag
    34441 gtcgccaagc aagattaggt tttccaagtt cccctgttgt tgaaaataag gttatgatta
    34501 attgattttc tatacgttca tactctttta aaccatctgc ataaaacgta aaataatgat
    34561 cattttctgc cacagagaca ctcttatcga atatttctaa attaacaggt ttctctacca
    34621 tagtttctga ccaattcgga ggaatgacat caggggtatt ttcaataaat ccgttttgaa
    34681 tttgtgcaat tgatttttca gatttatcta aaacattaat tgctaagcgc atacgatgcg
    34741 agtaaacagt attatgaatt ttgagtgtgc cttcaacgat ttgactttct cccagactga
    34801 acgatatttc ataagatatt tcttccattt cgggtttttc agataaacga tcttttaaat
    34861 ctttaggaag ttgagctttt ccgtaaacaa ctaatgtttc ttgtaaagaa tctttatata
    34921 ctttagctgt ttctaatttt agagatagtt ctgtatctcc ttctaatggt gaataatcat
    34981 acgtatcgcc atcatttgct gaatcgatca aatgaacgaa atcaagatat tgtctatctc
    35041 caactatcaa attcaattgc ccatctttaa aagaaagttt atatttattg ttacttatct
    35101 cagttttgtc tatctcgatt gctgtttcta atttttcatt atttttctca aattcaacaa
    35161 cagtgtatcc tagtgctggc acatttacat ttatttttat gtcaagttca taataagctg
    35221 gttcatcaaa atattcgaaa cctgtagcaa tcatccgata agcatttttc cgttctggaa
    35281 aataaacttc tttttcaatg atattatagg gactaccttt gaagtgtatt ttctttgaat
    35341 cagctactac atgcacgatt ttcgatccac tataattctc agtcgttgtg ttgaaaacaa
    35401 ggacttcatt cgattttagc tttaatgcat cagccatccg ttttacaata aggttttcga
    35461 ttccgtcagc tatctcatta gcttctttga ttcgatggaa aatatcttcc gcaacattat
    35521 ctgaaacaca gccaccaata ctatcatgag cctggttttg caaaatcttt ttccataacc
    35581 gacgaagtag gcctttactg atatggatac catttctttc tgcaatcagc atgagcggct
    35641 caactcgttt tagaattttt tgttccaatt taaaattttc tattttcatt tttgttctta
    35701 ctgagccaat agaacgatga acacgagagt aaaccggctg acgaagttca cctttatatt
    35761 caggtaattc ttttttgttt ctgataactt ctacataaga aggataatct gaaataatat
    35821 tgtggaattt actgtattta ttcaacttct ctacgacttg atcaaaattt aaaatcattg
    35881 acttttgatc tacacccgaa ggtactaaaa tatctttgtt ttttccttgc atatgaagca
    35941 tttcaactgc ttcatccaac ttttcttttg caaattcagg aatatcttta attgcgtcta
    36001 aggtcatcat tccagtcata taaccaaatg gaaaattcat ggcataaaca aatttatctc
    36061 ccaatccttt ccagataaaa taaggagatg gaaccatcgt ttcaaagttt aatcctcgcc
    36121 aacacatgaa attatcgatt tctacttgat gcaaaagtgt aggcaaattc gcattgaacc
    36181 caaacgtgtc aggcaaatac cctaccatca ttggatctcc atatttaagg atcgtatcac
    36241 tgattccaat cattaagttt cttaaaattg attcagcatc cactaataag gcatctgttt
    36301 gtgcatacca cggtcccacg aatagttgtc ctgttttcat cagtcgttgg ataaccttaa
    36361 gcttttgcgg attgatctcc aaatactcat cgacgattga agtttgtcca tctaaacaaa
    36421 agtttacttt agggttcctt tccaattcat ctagaacttc agtaaagacc tgatcagaca
    36481 atacgatggc atcttcattt gtaaaatacc attctctgtc ccagtgcgtg tgttgaatta
    36541 tatgtgcatt taccatatca gattctccta ctttccttct tctactgaat ttacttgttg
    36601 ttctttttta tacttgatgg attttagtgc attgaccatt aacgctgtaa ccactccccc
    36661 aataacaatt gatactaaat ataaaggtat attcgtaaca aaaggaattg ccataaaggt
    36721 tgaaagcatt gtttcttgag taacaccaaa aaatccattt agaccacatg ctactgcagt
    36781 gcccaccacg atgcttggta ttactcgtaa aggatctttc acggcaaacg ggatcgtccc
    36841 ttcagtaatc cccacaaggc ccataatcat agctgtcttt gcattatctc gttccgattc
    36901 atcataaagt tttttgttta aaattgttgc taaaaacatt ccaaatggcg gaatagcaat
    36961 tcctaataac atggctgtac taggtccaaa gcttccgctt gaaaaagaag caaccccaaa
    37021 agcatatgcg attttattga taggtcctcc catatcaaac gccatcattc ctcctaaaat
    37081 agccgacatc aaagcagcat ttgctcctga catgccttct agccaattag tcagtcctgt
    37141 tgtcattgct tgaataggcg tagctagtac ataccataat atgattccaa caactaatgt
    37201 agtaacaaaa ggaatcagca taattgattt tagactaata acttgcatcg gtacttttac
    37261 ttttttcaat gcattagcta aatagccaca agctaatcca gcaaccattc ctccaataaa
    37321 tcccatacca agattttgac acatcatccc agtgataaaa gctggtgcta gaccgggacg
    37381 gtcagcaatt gaatatgcga tataggctga aatcatcggt acgataaact gtaatcccgt
    37441 ctgaccaatc attcgcagga ctccccagaa tgtatccgtc tggtcccaaa cacctgtgcc
    37501 accaaaaatg actgcaatag aagttagtaa ccctgctgct acgacaagcg ggatcatata
    37561 ggaaacccca ctttggaaat gatttagaat ttctcttcct tgtttcttga attccattag
    37621 taactccccc acgatatatt ttttatttgt taattaatgc aattgcttca tcaatcgtct
    37681 cttcaccatc ttttactacc tctgaggtcc ctactgttaa aatcggttta ccgtcaaagc
    37741 gacctcgttc tgaaacattt gtatctactg caaatatgac aacatctgcc tcggaaacat
    37801 ccgaaattga aagtttgttt tctatcccca tagccccctg tgtttctact ttaatatcta
    37861 accctttctt cttcgcaaac ttttctaaat tagcttttgc catataagta tgggcaatcc
    37921 ctgcgataca tgctgttact gctactattt ttgtcatatg aaattcctcc ttttaaaatg
    37981 atagagcttt taccaattct tttttatttc tcgaatcttt tattctttta acaaactcgt
    38041 catccatcaa tcttccagat aattctgcta aaatacgtaa atgattagta cctttatcag
    38101 tgtcttttat tgctaacaaa aagacgagat caactggttg atcatctaat gcgccccatt
    38161 caacagaccg ctttaacttg gctacagcca ctgttgattc aattactgct aggcttttgc
    38221 catgaggaat ggcgatattt ttcccaattc ctgtggtcat atggttttct ctgtcaaata
    38281 cgtctttttg aaattgtttg tggtcagaca caaacccatt agtctctaac aatttcgata
    38341 atttttcgac tacacttttt ttatcagatg catttaaatc tatcactata ttatttatat
    38401 tgattttttc actgatcatt tataagcctt ctttcaatat ataaactaat ttttcaaacg
    38461 acgaactcgt ttttaaattt gctaataact catgattcga agcgatttca ttgaatgttt
    38521 cataaatttt attcaattca ggttttactt tttcattaag tgcaaaaaag aaaacagaat
    38581 gaacaagtgt cccatcccaa tcaattcctt ctggattaac gtagacacag attactggct
    38641 tttttataaa atctacttcg atatgcggca tagcaaaatc attcatcgct gttgaagata
    38701 gtttttctct tttgattgct ccctctagta tcccttcttt cgcgtaattt tcttttacca
    38761 attttttggt aattatattt aaaacgttca cttgattatc ctttatttgg tgggtaaaaa
    38821 agcaaaatcg tttatctaat aaatttagaa aagcatcttc tgtttgttca tttaactgta
    38881 gatactgttt tacttttctg acatctgccc ctaacatcaa aggactaaca acaataacag
    38941 gaaaatttgt attttcaatc ggaatcgtgc ttataacaag ttcatcagtg acttttctat
    39001 cttgtagttc attgatgctt agtacatctt ctatctcaac catatctgca aaaccgttat
    39061 ttattctttg ctctaataat tttgatgttc catatccgct actacaaact aatatcactt
    39121 tcgttttgtc aggtttgtaa cgatccaaaa gtgattgaat atggagtgtt acgtaagcga
    39181 tttcatcttc attaaaatgt atacagaatc tctcttcgat ttcctctaat aaatctacac
    39241 ttatcataaa tgacctttta aaagaatatt tgattttgtc cgtataaggg ttatagatat
    39301 ttactcccaa tttgagtctt ttaactgctg cattgagatg taatactaga ttattgatca
    39361 attcttcatc aggataaaat tcatttaatt taattcttac taactcttgt agctcaacac
    39421 ccatatctaa ataggaaagc ttcgtttgat ctaagtcgtg attcatagca tttttttcaa
    39481 tcgaagtaat gtgaatgtca acataagctt gttcatctat tggcacaata attgaaaatg
    39541 ctttttcaat cattttgatt aaaataagtg tattctttga gatttcttct tcagaattat
    39601 tactacgtga taagaaattg cttgtttcaa tcctttctat cgcaatagca aggtggatag
    39661 ctaaagtttg aaattcataa tctgtaaatt tcaattgtgt cttattcatg tatcgattaa
    39721 gcaattcggc tacttttttc aatatgtcct tatttaagaa attattcgct tcaccaatga
    39781 aattgatatt caagtctaag agatctgttt tactgttaga tgctgtaaag ccaccccaat
    39841 aatagttcac aacttctgac atcaaccttc ttttactttt ttcatcagct ttgatgcgca
    39901 cgccatttcg atctgaaact aaggacatcc catgcttgtt tagtcttttt ctaacatctt
    39961 tcattgtgct ttctaccgtc gacctgctca cgtataattc gtcagcaaga ttttgaatct
    40021 ttagagaatc attagtattc aataatttag caaacacata agttagacgg tcctccggag
    40081 agtctgcgat attgatttta tgactggata ataaatttcg aagtgtttct ttttttccaa
    40141 taagcgaaac gcctttttta ggttgaacca acatttgtac atctggcatt ttttggatta
    40201 tttctttcaa aattaaaata tctttgctaa ttgttttcgt agatacatat aattgttctg
    40261 ataattcttt gtaagttgtg tgctcatttt caatcaaaaa gtatagtatt ttcatttgcc
    40321 gttcgctaaa ttcgatgccc attatcttca cctcctgaat acagcttaag tgtacaatta
    40381 ttatctctat tttcatacag aagaaagttc ttgattaatg tagaagtttc taatattctt
    40441 gattatttca aatcaataaa aaagcctgca atatttatat cacaggcttt tttctctatt
    40501 aaatatatat aagataatac atttttctac tagttctcac taatcttgta taattaaacc
    40561 ctccgcaact tgacggagat ggagaagatg cccccgtcta atctagcggt catctctcct
    40621 cccattcctt ccaccaattt ttttgacagg taaagcccga ttccgctaga ttccgtattt
    40681 gtgcggctgg tattttcggt ataaaaacgc tggaagactt tttcaacaga ggtttgcgtt
    40741 cctggtttga taggattggc cactgttaat cgaatgaatg gatcctcttt tgaaaatgtc
    40801 acctttgctt gtccatctgc ataacgcaat aaattctgta tgatattttg cagcactcgc
    40861 agcaacatga gcggatctgc taatacagtt gtatttatcg agtgttcagc gaacgctacc
    40921 tcgatatttt tctcagaaaa ttgttcataa aaagagaaaa atacttcttc cacctcttta
    40981 gaaaaagaaa gcgtatggag atccatttct ttttgcttcg tctctattcg agcaagatca
    41041 taaaactgat ccgtcatact gagtaatctt gcaacagatt gtttgatgat cgttaaatac
    41101 ttctcccgtt tctccgtatc ctctacctgt tccagtaatt gtacataacc gttaatagca
    41161 gtcaaaggag ttctgagatc atgagaaagg ccgacgatcg atagtctgat gttttcttcc
    41221 atttcttgaa atgcttgtcc tttttgttca ttttcatcca accatgtatt cagtgcatct
    41281 actaggtcca ttagattctt ttctcgaaat tcgactgaaa gacgaccacc atattcttgt
    41341 cgttcagaaa gagtggcgat tttcttagtt aatctcttga tttccctttg caggaagaaa
    41401 ataaaacttc ccataaagat aacaacgaga agcaacagcc atttaatcaa atctaaaccc
    41461 cactccccac accgtatgaa tatacacagc atgtgccttt gctagcttcc ctcgaagatt
    41521 ggaaatatgc atattcactg ttttctcact atcaaaatat ggttcttgcc aaactgtttc
    41581 ataaagattt gctcgcgaaa atactttttt cggatgttct aaaaaaagct tcagcagatc
    41641 gaattctctt cctgtcaaat ggacagccac accattcaca tttacatccc tcgactcttt
    41701 gtccagtaag atttccttgt aatagatgcg ttgcgacaga tcttttactt ggacagaagt
    41761 ctgatgacga agttggatgc cgattcgtaa caaaagttct tcgatatcaa atggtttcgt
    41821 cacatagtca tccgctcctg ctgccaacaa ggaaacttta tctctttgat cgctttttgc
    41881 tgaaagaacg atgatcggaa cattcgatgt ctgtcggatt tctgctaaca atgcttcacc
    41941 tgaaagccct ggaagcatga gatctagaag gatcaaatcg acaggatatg tgcttaacaa
    42001 taattttcct tccgtacctg aataagcatc aagaagtttg tatctctgtt caagaacctc
    42061 ctccagtaag gaatgaacag cttcatcgtc ttcgataatc aaaatagttt ccagcaggac
    42121 cacctactta atgtcagatt tttggaaaat aaacattcct agagctgatg ataccacgat
    42181 tgttactaga ctgaccagaa tcattttcgg tgcttgcgaa taagttgctt gttgcgcaaa
    42241 gtccgtatac atgagccacg tttccgtaaa ccactccgtt agaaattcca agcctttgat
    42301 ttttgcgaaa acaccgggaa tgaaggccgt tcccatgata taaagaaaag aaagaagcaa
    42361 actgacactg gtacttctag tgatgaatgt caaagccact ggaacagata atgtccctaa
    42421 tgtcagcaga agcaatacag gaaatacttt tagaacataa tttccatagt ttttgattcc
    42481 ctcaacttca ccaaatccga aagcaagtgt gtaagcaagc atggctagtc ccgtgatcaa
    42541 aaccacgcca agtgttgtga tcaaaagcgt aacgagaagt ttcgacacat aataagtcat
    42601 tcgattccgt cctaaagata acgtattgcg aatcgtaccg ttttgaaatt cttccccaat
    42661 ataaaagcac gcaaatgctg cgaaagccag aagaatccat gcagccattt tttctaccat
    42721 taaaatccct gttgcaccgt tgatgactgt atccgttgct tggtttaatt cattgccgta
    42781 ttttgtatgc atataccatt cgttccaaga ataaaaaagc ggaagtacga ccatcagtcc
    42841 tagtgagacc cacattttac ggctcttcca tacttttatt ttttcagcag ttaatagatt
    42901 aaacatgtgg attgcctcca gttaattgta agaaataatc ttccagtttt tgcccaacta
    42961 ggtgaatacc atccacagga acttttgctt ctaccaatgc ttgattgatg gctgctactt
    43021 ggtcaattcc ttcaaaaagc tggataacac ggctagattg tacaaaataa tccctataac
    43081 caagagagtc gagaaccgtt actgcttgtt ccgtcgccga agtgtcaatt tttataaatt
    43141 gacgagattc ttgtgccaat tcttctttcg ataattcttt gatcaagtgt ccttcatgca
    43201 aaataccata gtgagtggcg atctgtgaca attcatctag caagtggcta gaaagcaaga
    43261 cggtgatgcc ccgttctttt accaaacgtt ggatgatttc tcgcatttct gccatccctg
    43321 ctggatccat cccattcgtc ggttcatcca aaatcaaaaa ttctggattc gtgatcagtg
    43381 catttgcaat agctaaacgc tggcgcatac ctaaagagaa attctttgct ttctttttcc
    43441 ctgtattttc caatcgcatc attttcagca ggtcttcgat ttctctatcc gacacaccgc
    43501 cgttcgctgc ttggatacgt aggttatctc ttgcagttaa ttctggataa agagcagggg
    43561 tttcaatcga ttgacccata cgtcgtcttg ctgcctgcaa atccgtcgtt ccaaataatt
    43621 cgatcgaacc ttcatcaatc gagatcaaac ccataatagt tctcataaag gtagacttac
    43681 ccgctccatt ttccccaatc aatccataga tcattccacg tttgatttcg attgatactt
    43741 tatctaatgc tttggcagca ccgtattttt tggttatacc agttgctttc aagaccgttt
    43801 catttccaaa atccatactt atccttcttt cttattgatg agtgaagtat agttgacggg
    43861 tataaagaaa gcagccagaa atgtataaag aaagtgtaaa ttttccaatt gaatgtcaaa
    43921 aaaaacaatc ggacaatacg tgtctttcct attatcaggc agtttgttat acgcttgtat
    43981 cagtttcctg attctttctt ttcgaccaaa gtcgtttctc tgtattcctc ttcactgaaa
    44041 atttttgctt ctttcagatc aagcttgagt aattttacca gaccggttat aaggataact
    44101 atagcaagac tccctcccag aaaaattaga ctgtctcgca gacgtggttg cgttgtcttc
    44161 caatcaaaga gcgcaccaaa cagtgctaat ccaagtggac tggccaccat ggtaatcgtt
    44221 gttatcgttg acataagtcg tccttgtata tgcgttggaa cttctcgctg aagccagacc
    44281 atataaaaag caaaagaaaa cattaatcct taaaaacgga cttatgaaaa ataatacatg
    44341 ccgtttggga tttacaatta gctaccaaat aagaggttat gacaaaagac ttttcacaat
    44401 cccttcttcc gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattt
    44461 atcaaacatg agaagcaatt tttgtaaatt ccttcttatt tcttgaagct gaccacttct
    44521 gtcacaacct cagaagcgga taaataatat tgtttgatga tactttatct ttttcccgtt
    44581 tgtttataga tcgaatgata ttatagacca agaatcctac ctattaacat agctaaaaac
    44641 acaaaaaagg cactttccaa attgtggaaa gtgccagaaa agttaggatt aaggtaactg
    44701 cgctcagcat tgcttcgccc agtacgattc tcgtaactcg tgaactcgaa cgataatcgc
    44761 aattaacgtt ttgagaactg tgaagcttta cgggctttct taagacctgg ttttttcttc
    44821 gtatcattga ataagtttga aaaaccttga tttataaagg ttttcccgta aatatatttt
    44881 attatatt
//
LOCUS       DAYYCQ010000015        39058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000015 DAYYCQ010000000
VERSION     DAYYCQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.015"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..616
                     /locus_tag="ACHAYF_000844"
     CDS             <1..616
                     /locus_tag="ACHAYF_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8578303.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="ACHAYF_000845"
     CDS             613..1386
                     /locus_tag="ACHAYF_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8578304.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="ACHAYF_000846"
     CDS             1383..1781
                     /locus_tag="ACHAYF_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8578305.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="ACHAYF_000847"
     CDS             1966..2637
                     /locus_tag="ACHAYF_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578306.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="ACHAYF_000848"
     CDS             2696..3136
                     /locus_tag="ACHAYF_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578307.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="ACHAYF_000849"
     CDS             3148..3816
                     /locus_tag="ACHAYF_000849"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8578308.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="ACHAYF_000850"
     CDS             3880..4503
                     /locus_tag="ACHAYF_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8578309.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="ACHAYF_000851"
     CDS             4559..4945
                     /locus_tag="ACHAYF_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8578310.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..6549
                     /locus_tag="ACHAYF_000852"
     CDS             5158..6549
                     /locus_tag="ACHAYF_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8578311.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7160..7528
                     /locus_tag="ACHAYF_000853"
     CDS             7160..7528
                     /locus_tag="ACHAYF_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8578312.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7708..8649
                     /locus_tag="ACHAYF_000854"
     CDS             7708..8649
                     /locus_tag="ACHAYF_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8578313.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8718..9449
                     /locus_tag="ACHAYF_000855"
     CDS             8718..9449
                     /locus_tag="ACHAYF_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8578314.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9593..11773
                     /locus_tag="ACHAYF_000856"
     CDS             9593..11773
                     /locus_tag="ACHAYF_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8578315.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11793..12275
                     /locus_tag="ACHAYF_000857"
     CDS             11793..12275
                     /locus_tag="ACHAYF_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8578316.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12291..13052
                     /locus_tag="ACHAYF_000858"
     CDS             12291..13052
                     /locus_tag="ACHAYF_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8578317.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13183..14517
                     /gene="murC"
                     /locus_tag="ACHAYF_000859"
     CDS             13183..14517
                     /gene="murC"
                     /locus_tag="ACHAYF_000859"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8578318.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14824..15309
                     /locus_tag="ACHAYF_000860"
     CDS             14824..15309
                     /locus_tag="ACHAYF_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8578319.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15302..15985
                     /locus_tag="ACHAYF_000861"
     CDS             15302..15985
                     /locus_tag="ACHAYF_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8578320.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16111..18756
                     /gene="polA"
                     /locus_tag="ACHAYF_000862"
     CDS             16111..18756
                     /gene="polA"
                     /locus_tag="ACHAYF_000862"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8578321.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18801..19637
                     /gene="mutM"
                     /locus_tag="ACHAYF_000863"
     CDS             18801..19637
                     /gene="mutM"
                     /locus_tag="ACHAYF_000863"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8578322.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19601..20230
                     /gene="coaE"
                     /locus_tag="ACHAYF_000864"
     CDS             19601..20230
                     /gene="coaE"
                     /locus_tag="ACHAYF_000864"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8578323.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            20282..20614
                     /locus_tag="ACHAYF_000865"
     CDS             20282..20614
                     /locus_tag="ACHAYF_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8578324.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20926..21417
                     /gene="nrdR"
                     /locus_tag="ACHAYF_000866"
     CDS             20926..21417
                     /gene="nrdR"
                     /locus_tag="ACHAYF_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8578325.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21440..22813
                     /locus_tag="ACHAYF_000867"
     CDS             21440..22813
                     /locus_tag="ACHAYF_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8578326.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22813..23739
                     /gene="dnaI"
                     /locus_tag="ACHAYF_000868"
     CDS             22813..23739
                     /gene="dnaI"
                     /locus_tag="ACHAYF_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8578327.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23868..24308
                     /locus_tag="ACHAYF_000869"
     CDS             23868..24308
                     /locus_tag="ACHAYF_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8578328.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            24452..25153
                     /locus_tag="ACHAYF_000870"
     CDS             24452..25153
                     /locus_tag="ACHAYF_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8578329.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            25150..26271
                     /locus_tag="ACHAYF_000871"
     CDS             25150..26271
                     /locus_tag="ACHAYF_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8578330.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            26286..27518
                     /gene="pepT"
                     /locus_tag="ACHAYF_000872"
     CDS             26286..27518
                     /gene="pepT"
                     /locus_tag="ACHAYF_000872"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8578331.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            27650..28558
                     /locus_tag="ACHAYF_000873"
     CDS             27650..28558
                     /locus_tag="ACHAYF_000873"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8578332.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            28593..28865
                     /locus_tag="ACHAYF_000874"
     CDS             28593..28865
                     /locus_tag="ACHAYF_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8578333.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            28876..29922
                     /locus_tag="ACHAYF_000875"
     CDS             28876..29922
                     /locus_tag="ACHAYF_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8578334.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            30173..32782
                     /gene="clpB"
                     /locus_tag="ACHAYF_000876"
     CDS             30173..32782
                     /gene="clpB"
                     /locus_tag="ACHAYF_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8578335.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(32933..33619)
                     /locus_tag="ACHAYF_000877"
     CDS             complement(32933..33619)
                     /locus_tag="ACHAYF_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578336.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(33597..34091)
                     /locus_tag="ACHAYF_000878"
     CDS             complement(33597..34091)
                     /locus_tag="ACHAYF_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8578337.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(34111..35331)
                     /locus_tag="ACHAYF_000879"
     CDS             complement(34111..35331)
                     /locus_tag="ACHAYF_000879"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8578338.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            35478..37313
                     /gene="lepA"
                     /locus_tag="ACHAYF_000880"
     CDS             35478..37313
                     /gene="lepA"
                     /locus_tag="ACHAYF_000880"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8578339.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            37509..38420
                     /locus_tag="ACHAYF_000881"
     CDS             37509..38420
                     /locus_tag="ACHAYF_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578340.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        13056 a         6733 c         8431 g        10838 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     6181 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     6241 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     6301 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     6361 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     6421 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     6481 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     6541 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     6601 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     6661 ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     6721 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
     6781 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag
     6841 agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt
     6901 tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt
     6961 tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa
     7021 acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag
     7081 ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
     7141 atcgtttagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt atgtggacga
     7201 tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag atcgtcaaga
     7261 aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct catttctgtt
     7321 atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg ccccatcttt
     7381 gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc aatcagtcac
     7441 attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg atcccgatgg
     7501 caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa
     7561 caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa aactttccat
     7621 gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta tactgtttag
     7681 gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta ttacaattat
     7741 acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg caaatctttg
     7801 gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt gtctggaacc
     7861 gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt tgctgatacg
     7921 atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt aagataccgt
     7981 ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt gacaggtgcc
     8041 tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt ggtgattgat
     8101 gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg cccagatata
     8161 cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt tatggaaaaa
     8221 cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg ttggcttgtc
     8281 cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta tggtaaggat
     8341 cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga acggatcgct
     8401 gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga cggaacacca
     8461 tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct tgcttccatt
     8521 gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat
     8581 cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt tacagcttta
     8641 attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct atactagcgt
     8701 tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc catggcaaac
     8761 atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag cttgttttat
     8821 taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta ggaaagcaat
     8881 tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat tttttgcaat
     8941 ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga aaagaaacgt
     9001 ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa atctcgatga
     9061 tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc ttgtattatg
     9121 aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa gactggcgta
     9181 agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg aaaaacaata
     9241 caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga gatatgcaga
     9301 caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct gtattcggag
     9361 ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt gaatatcaga
     9421 acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag ctgtgacttt
     9481 gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt aacccaggat
     9541 aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt taatgacgga
     9601 aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa
     9661 gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg
     9721 ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg
     9781 gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc taatcgatga
     9841 acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga aaatgcaaca
     9901 agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa cgattgctaa
     9961 agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg gagaggtgca
    10021 atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt
    10081 gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg
    10141 gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc
    10201 agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc ataaaatggt
    10261 ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga aagtattctt
    10321 tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca aaaatcctgc
    10381 ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac gctttattca
    10441 gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg agcaagcaat
    10501 cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt
    10561 tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac
    10621 agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc aagcaaaaag
    10681 agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag atatggtagc
    10741 tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc agttgaaatc
    10801 cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg tttactccgc
    10861 tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa gtgctgtttc
    10921 tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc ctaaagctgt
    10981 cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac aattggattt
    11041 tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa gcccgcagct
    11101 cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca tttatcgtga
    11161 agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct taggaccaaa
    11221 agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca ttttggacaa
    11281 cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt cagtccaact
    11341 ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat tatccgtaga
    11401 gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt tagctgggct
    11461 gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac gtacagatgt
    11521 attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc gtaatgtcat
    11581 tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc atatatcgaa
    11641 actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag atatcgtaac
    11701 agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga tgctttcccc
    11761 ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg cagcagttag
    11821 tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca atgttcaatc
    11881 cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta gattttaatg
    11941 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
    12001 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
    12061 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
    12121 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
    12181 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
    12241 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
    12301 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
    12361 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
    12421 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
    12481 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
    12541 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
    12601 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
    12661 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
    12721 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
    12781 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
    12841 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
    12901 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
    12961 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
    13021 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
    13081 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
    13141 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
    13201 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
    13261 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
    13321 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
    13381 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
    13441 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
    13501 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
    13561 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
    13621 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
    13681 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
    13741 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
    13801 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
    13861 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
    13921 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
    13981 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
    14041 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
    14101 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
    14161 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
    14221 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
    14281 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
    14341 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
    14401 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
    14461 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
    14521 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
    14581 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    14641 caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta tttgatgcag
    14701 gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg gagcagaact
    14761 tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg gagggtttct
    14821 atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc
    14881 gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta tttgggattg
    14941 acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac agaatatcaa
    15001 cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat ttcaaagcat
    15061 cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga accggttttc
    15121 cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat gaaggatgtg
    15181 gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt
    15241 atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg
    15301 aatgaataat ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg
    15361 atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc tttgggtatt
    15421 tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg gtctgtggat
    15481 cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc cgagcttagc
    15541 aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg ttactcttgc
    15601 atactacgat attggaacag taacgcaagc tttcttgaca gctactttga tgttcatggg
    15661 gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa gagcaggaca
    15721 ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt tgagaagtag
    15781 cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga ttacagctta
    15841 tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac atagtggaat
    15901 tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct tggcgtttct
    15961 gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg gctataccga
    16021 attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt gttagaatga
    16081 ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt actcgttgat
    16141 gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga acgtttcaaa
    16201 aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt
    16261 atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa aacgactttt
    16321 cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa
    16381 gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta ttacgaattg
    16441 cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga caaagatcaa
    16501 tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag taaagaagta
    16561 aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc agaacatgta
    16621 gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg actagccgga
    16681 gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta
    16741 ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc
    16801 aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg
    16861 accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta tgaagggaaa
    16921 aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt cctttctaaa
    16981 ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa
    17041 ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat gggcgacaat
    17101 tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat ttatgtcacg
    17161 aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga tccgactcgg
    17221 ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta tactggaaaa
    17281 acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa
    17341 agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca atcagatgaa
    17401 gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact cttttttgct
    17461 catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag ccaagaactg
    17521 gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc tatcattttg
    17581 gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga aatgaagggc
    17641 gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc cggcgaagaa
    17701 ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat gggattaccc
    17761 gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga acagcttcgt
    17821 gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa aattcaatct
    17881 acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca tacccgttat
    17941 gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt gcagaacatc
    18001 ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag agaaaaagac
    18061 tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc gcatatctca
    18121 aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc cagcacagcg
    18181 atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg taggcaggca
    18241 aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc acaaaatcta
    18301 ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa atacccaggt
    18361 gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg
    18421 ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa tatccgttct
    18481 tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga tattttgaaa
    18541 atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc tacgatgctg
    18601 ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga atcattagat
    18661 aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact gatcactgac
    18721 agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac agagcaatat
    18781 aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt cgtaaaggat
    18841 tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg cccaagatca
    18901 tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc atccaatcca
    18961 ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg atctcacact
    19021 tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat aaacatacgc
    19081 atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta cgaaaatttg
    19141 gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg
    19201 gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga ctgaaaaagt
    19261 cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt ctaggtaata
    19321 tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg gcgaacacac
    19381 tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg gatcgtgcaa
    19441 tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa tcaggcggat
    19501 ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc
    19561 cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc
    19621 taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta ccggtaaaag
    19681 tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg atgtgattgc
    19741 cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag tgtttggcca
    19801 agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga ttgtttttcc
    19861 atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc gaaaagcgat
    19921 aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag atatcccatt
    19981 gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt atactacgcc
    20041 agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac aagcgcagca
    20101 gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata ttttgttcga
    20161 caacaatgga acaaaagaaa tacttgtggg acaaatcgaa aattggcttt ttgctaatcg
    20221 ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt gagggatagc
    20281 gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg
    20341 cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag aaacagcaga
    20401 agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc aggcagaaga
    20461 agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa
    20521 aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg aacagcgaat
    20581 gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga gatgtgactt
    20641 gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga ctaaatcagc
    20701 ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt gaaatttagt
    20761 aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt
    20821 ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga
    20881 aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag atgtcccaga
    20941 tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg acgtgcgatc
    21001 cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga acgaattgaa
    21061 gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa
    21121 attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga acaaatggaa
    21181 caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg
    21241 aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat cgcttatatt
    21301 cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa
    21361 gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa caattaagaa
    21421 acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa aaaatattta
    21481 ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc tgtatcttta
    21541 tcagccacta atcggcggag aagccttgag tatctatttc accctgttat cagaaatctc
    21601 tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta gtctaggctg
    21661 cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct tactggatgt
    21721 ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt tagaaccact
    21781 cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg aaaaagttgg
    21841 tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata cagcagaggg
    21901 atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc
    21961 tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg aatcagtagc
    22021 attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc tggcaaaaaa
    22081 acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc atcagcttta
    22141 cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact ttagtgaaca
    22201 aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac gtccggtgaa
    22261 gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt caaacgaaca
    22321 tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac cgatgaaata
    22381 tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa actggttact
    22441 gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt tgctgaatta
    22501 tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata cagtggcaaa
    22561 tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca ttcggcaaat
    22621 aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg
    22681 aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat
    22741 cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg caaaaaagga
    22801 gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat
    22861 tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga tgttcaagct
    22921 tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag ttacgctaaa
    22981 ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct
    23041 cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac ttatgtacca
    23101 acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg
    23161 gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac agatggacga
    23221 ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga tccaaaaggg
    23281 tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata tttattagga
    23341 gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca ttttccgaca
    23401 tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa gttagatgca
    23461 gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat gagtagttgg
    23521 atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca gttaccaacc
    23581 ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt cactcaacgt
    23641 ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta tttgacaaaa
    23701 gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa gtccaactga
    23761 catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa atgaccggag
    23821 acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg agggctattt
    23881 tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt agcgcagata
    23941 gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca agcagtacga
    24001 aaacggagag acaaagcata tttgtcggaa cgattgaaag tatcactcaa gtagaagcag
    24061 aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat ccagcgaata
    24121 tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa aaaataagcg
    24181 tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt gagatgccga
    24241 tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat gtttcattga
    24301 acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg
    24361 tatatttttt aaggagatat gggaattttt ttattcctag tctccttatt ttatttggaa
    24421 aatgctataa ttatctagaa ggagtgaagc tatgaaccat ttagatttat ctcagcgctt
    24481 agccgcagtg ggtgactttg tccctgaatc cgctcgttta gcagacattg gatcagatca
    24541 cgcttactta cccgttgcat tgatattaag aggaaaaatc gaatatgcag ttgctggtga
    24601 agtcgttcaa ggtccttatg aatcagcacg taagcaagtc aaaaaagatg gattagaaga
    24661 ccgtatcacc gtacgccttg caaatggtct agatgctatc cagccacaag atggtattac
    24721 agctgtcacg attgcgggta tgggaggttc actgatccga gatatcctag aagctggcaa
    24781 gttgaatcaa cgcttaagcg gacaagaacg tttgatcctt cagccaaaca tcggagagcg
    24841 tacattacga tcttggctgc aagaaaatca gtataaaatc caatcagaaa aaatacttga
    24901 agaaaataaa aaaatatatg aaatcattgt agccgaaaaa gaacaaaaac cagttactta
    24961 caatgaaatg gagctgatgt tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa
    25021 atcaaaatgg acaagagagc ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc
    25081 agctaatcaa gaggcacgtc aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt
    25141 tttaaagcca tgacaatcag cggacgcact ttcatccaaa agtttgaaag ctattgtcct
    25201 cagtggctag ctgaagaagg agacccagtc ggactgcata tcggaacatt ggataagcca
    25261 atccaacgag taatgatgac attagacgtt cgtccagaag tggtcgaaga agcaatcaag
    25321 aaaaaaatcg atttgctgat tgctaaacat cctccaatct ttcgtcctgt aaaacggctg
    25381 gttactgatc agccacaaga aaaaatgtat gcagatttgt tgaaacatga tattgccgtc
    25441 tatgcagcac acacgaacat ggatattatt tgggatggac tgaatgactg gttttgtgaa
    25501 cttctcggta ttgaagtgga gagttattta gtaaagactc atgagattca ttataaaaaa
    25561 ctggccgttt atgtacctgt ggatcatgca cagaaaatgc gtgaagtatt agctgcagct
    25621 ggtgctggta cacaaggtga ttataccggt acaagtttta cctcgattgg acacggtcga
    25681 tttactccgg aagctggtgc acaaccagcg attgggaaag tagggaagac agaacaagtt
    25741 caagaggcaa aagttgaggt tattttgccg gaaactatcg aaaaacaagt gattcaagcc
    25801 atgcggtcag ctcatcctta tgaggaacct gcttatgatc ttttcgctat agatgaacca
    25861 gtcgaaatgt ttgggttagg acgagtaggt gaattgccgc aagaaatttc tatagaggca
    25921 tttgtagaac aagtaaaaga agcgttccaa ctagatggac tgagaatagt ccagccaaaa
    25981 aatgcaaaat cctctgtgaa gcgcattgcg atttgcggtg gaagcggtga aaaattttat
    26041 ccgcaagcaa tcgcccaaag agcagatgtc tatatcacgg gagatatcta ttatcatacg
    26101 gctcatgata tgcaaagcgc tgggttgatt gcaattgatc ccggtcatta tattgaatcg
    26161 ttatgcaagc aacgatttat cgagaaattt gaatcttgga aacaagaaga aaattgggat
    26221 attaactttt tcgtttctga aacaaataca aacccatttc aatttaatta aaaggagaga
    26281 atactatgta cgaaaatctt ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct
    26341 ccgatgcaac aagcaaaacg actccatcga ctcagacaca agttgctttt gcgcaagaat
    26401 tgaaaaaaga attagaagag ttaggcttag ctgatgtttt ctataatgaa gataacggat
    26461 tcgttatggg aacattacca agcaatgttg atcatgaagt gcgttccatt ggatttattt
    26521 cccacatgga tacagcagat tttaatgcag taggagtcaa tccacaaatc attgaaaact
    26581 atgatggcga gtctacgatc gtactagatg cggaagaacg ctatacattg aatacaaaag
    26641 attttccaaa tttgaaaaat tatgaaggcc acacattgat cacaacagat ggcagtacat
    26701 tactgggagc agatgacaaa tcagggattg cggaaatcat gactgcaatg gagatcttga
    26761 tcaataatcc ttctatccct cacggtacca tcaaagtggc ttttggtcct gatgaagaaa
    26821 tcggtgtcgg agcggataaa ttcgatgttg agcaattcga tgtagatttt gcttatacag
    26881 tcgatggtgg tcctttaggt gagttgcagt atgaaacgtt tagtgctgct caagcaaata
    26941 ttacgatcca agggaaaaat gtacatccag ggacggcaaa agatacaatg atcaatgctc
    27001 tacagttagc aatcgatttt cataatcagc ttccagccga tgaagttcca gaaaaaacgg
    27061 aaggatacga aggtttcttc catttgatgg cattggatgg gaatcctgaa gaagcttcta
    27121 tgagttatat cattcgcgat catgatcgtg aaaaattcga agcacgcaaa gcacaaatca
    27181 cacatatcca agaagagttg aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt
    27241 atgatcaata ttacaacatg aaagaaatca ttgaaaaaga tatgagcatc gtggaattag
    27301 cgaaacaagc aatgatcgag cttggtatct ctccagtgat cgaaccagtt cgtggaggaa
    27361 cagatggttc aaaaatctct tacatgggta ttccgacgcc aaatctattt gccggaggag
    27421 aaaatatgca cggacgattc gaatttgttt ctttacaagt aatggaaaaa gcaacagacg
    27481 tgattgtaaa aattgcagaa ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg
    27541 acaaggcgtt cgtcccaatg atggaatgtc atctgtctaa ggctaaaggg caggacaatg
    27601 gaattggttg tcccgccctc ttttttgaag agtaaatagg aggtaaagaa tggaaaagct
    27661 gctagttttt tataacaaaa gttcagggaa agatgagggt gagaaattag cacgctggtt
    27721 tcaagattat gtaaaagaaa agcagccaga attatatgta gaactagctg aaactggccc
    27781 atcgattgat gatgcggaat tagtcaagaa agcagaagcc atagaagctg atactttagt
    27841 aattataggt ggagacggca cgattcatca tatcgtccaa gcgttccaaa aaacattatc
    27901 tcaataccat gtcggattac ttccaggtgg aactgtcaac aaccttgcac gtgttcttga
    27961 gattcccctc aaaaaagaag aagctgctga cgtgatcctg gaaggcaatg tacggaaaat
    28021 agactatggt caagtcaatg atgaagtcat tatctctaca ctgacaatcg gaatcttagc
    28081 agatacagca gtttggatca gtcaagaaga aaaacagcgt tacggctcgt ggatctttat
    28141 caagaagttt tttaagttat tgctgaagaa gaaaaaatat catttggatt tagaaacaga
    28201 cgaggagcat tggaaaggaa aatcccaact gttgacagtc acgatgtcta actccgtggg
    28261 cggttttaca aattttgacg actcagcgac cctagatgat ggaaaatttc atgtaacgat
    28321 cgtaccttcc ttagatattt tccgatttat tttttatttg cctagaatca tgagaggcaa
    28381 attctatacg atccccggca ttacttattt cacagccaaa cgcatgaaaa tccaagcgca
    28441 ggaaaaatct gtgggcactc gaacggatgg agacacgaca gatgatcttc cagttgaatt
    28501 atctgttgta tcaaaaggat taacagttta ttcaccaaag aaagaagaaa aagcttagaa
    28561 atagtagtta acagaaagtt ggaagaagag taatggatgt attgttacac aatcctttgt
    28621 ggttttacct tttcattgtg aatttttact tattttgtct gatggggtat gataaacatc
    28681 aggctagaac tagcggctgg cgtgttcctg agaataattt gatatttgtc agcctgttag
    28741 gcggtggttt gggaggattg atctctcgaa aagttttcca tcataaaact cggaaaaaga
    28801 agtttactat aggattcgct gcaggtgtac ttgttgattt gattctcatt tattttttcc
    28861 attaaggagc aggagatgtc gtctaaagga acgaaaacta aaatcattct gaatctgctt
    28921 ttgctgattg tgatttttgc cattatttat tatttaatca atcaatcatt tgcagatatc
    28981 tttgccgaat tgctgtctac cagtgttgtt gttttggcag cggctgttat attagggaca
    29041 gtttaccaaa tcgctgaagg gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat
    29101 tttacaacga aagatgggtt cttgacctct agctatattg cgttttaccg aatcgtttcg
    29161 tttggtacag gaacgctgat ctcggagatt tatttttata acaaaaaagg aatgaagcta
    29221 tcccagggag caggggtgac agcactccat atgattatgt acaaactggc agtgatcact
    29281 tatgcattag ctggcttggt catccaattc tctctttttt attctaatgg tccatcaatg
    29341 atctggttta tcatcgctgg tatccttttg acgatcgtga ttgttgcctt tttattggcg
    29401 atgtccgtca gcttgaatgt tcaaatattt tttgtcagct tatcgaataa gttatttaaa
    29461 agtgagcgtt tgcggaaatg ggtggataca tgcaatcagc agatctactc tttaagggaa
    29521 acggtccaaa ccattatcca agacaggact gcgctattgc ggatttatct atggaatatg
    29581 ttaaaacttg tattttggta tgtacttcct tatttgattc tgattgaaaa ccatcctaaa
    29641 atagattttc tactgaccat ttcgtttatc agctttgcag tagtgctttc aggggttatt
    29701 cctacgcctg ctggtattgg ttcctttgaa tttgtctatt tgttattatt cagaccagtt
    29761 gttggaacag ttgacgcagt ttcttcgctt ttactgtatc gtttcagttc ctttatcttg
    29821 ccatttctct atggatttgt gtatgtgatt gctgaacggc gaagagtgat caaagaagaa
    29881 ataatagaag taaaaaaaga gaataaagaa aatcaagggt aggtctttta taagatctac
    29941 cctttttatc aaatcaaaga aaaacgttat ggataagata attttacgtt tgtttggctc
    30001 tttttttata tacatacaat caattttgtt tttttaagat ttatataagt cgagaaaaaa
    30061 agtcaaaaaa ctatgtgtat attttgacca aaattgacca aagcgatata atagtttttg
    30121 taaagatcaa agaaataagt gatcttacct ttaattagga gggtatacct gtatgaatat
    30181 tgagaaaatg actacgacgc ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac
    30241 tcgaaaacat caagatatcg acattgccca tgtatggaaa atctttttac aatctaatca
    30301 ttttggtcga aatttctata cggatgccgg attggatgtc gaatcttttg aacatgaaat
    30361 tgatcgtcta ctggatgagt atccagttgt ttctggtgga aacgtccaat atggacaaaa
    30421 cctcagccag aatttctttc gattattgaa tgaagcagac caaatacgtg aaagcttcgg
    30481 cgacgagttt ctttcaacgg aagtcgttat tttagcgttg atgaaattaa aaaattatcc
    30541 gttaactgtt tatttgaata aaaacggatt atctgaaaaa gaattgcgta aaaacatcga
    30601 agagatgaga ggaggagaaa gagtgacctc acaaaaccaa gaagaacaat acaaagcatt
    30661 agaaaaatac ggcgttgatt tagtacagca agtcaaaagc ggaaaaatgg atccaatcat
    30721 cggtcgtgat gaagaaatcc gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa
    30781 tcctgttctg ataggagaac ccggtgtagg gaaaacggca atcgttgaag ggcttgctca
    30841 acgtatcgta cgcaaagatg tgcctgaaaa tttaaaagat aaaacgattt tttcattaga
    30901 tatgggtgca ttgatcgctg gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc
    30961 agtcttgaaa gaagtcaaaa aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca
    31021 caatatcgta ggggcaggaa aaacagaagg aagtatggat gccggaaatc tgttgaaacc
    31081 aatgcttgct cgcggtgaat tacatttgat cggtgccaca actttagatg agtaccgtca
    31141 atatatggaa aaagacaaag cgttggaacg tcgctttcaa aaggtattag tcaaagaacc
    31201 aacagtggaa gatacgatca gtattctgcg tggattaaaa gaacgttttg agatccacca
    31261 tggtgttaac atccatgaca acgcattagt agcagcagct actttatctg accgttatat
    31321 tactgatcgg ttcttaccgg acaaagcaat cgatttgatc gatgaagcca gtgcaacgat
    31381 tcgggtagaa atgaactcta tgccaacgga attagatcaa gttactagac gtttgatgca
    31441 gttggaaatc gaagaagctg cactgaaaaa agaatcagat gatgcaagta aaaaacgctt
    31501 gaaaaatttg caagaagaat tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca
    31561 gtgggaaaca gaaaaagaag aagtcaattc tgtttcagca aaacgtgctg aaatcgataa
    31621 agcaaaacac gaattagaag atgcagaaaa taactatgat ttagaacgcg ctgctgtatt
    31681 gcgtcatggt acgatacctc aattagaaaa agaattgaag gaattagaag ctaaagcaaa
    31741 agacagcgag attaagatgg ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt
    31801 tggcagatta acgggtatcc ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat
    31861 taaattgaat gaaacacttc ataaacgagt gattggtcaa gatgaagcag ttgatgcggt
    31921 cagtgacgcg gtaattcgtt cacgggctgg attgcaagac ccaaatcgtc ctctaggttc
    31981 tttccttttc ctaggaccaa ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga
    32041 gaacttgttt gactcagaag atcatatggt acggatcgat atgagtgaat atatggaaaa
    32101 acatgctgtg tctcgtctag tcggagctcc tccaggctat gtaggttacg aagaaggtgg
    32161 tcaattaacg gaagctgtca gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga
    32221 aaaagcgcat cccgatgtct tcaatatctt gttacaagtc ctagatgacg gacgcttgac
    32281 tgattcaaaa gggcgagtag tagatttcaa aaatacggtt ctgatcatga ccagcaatat
    32341 cggttcacag ttactgctgg aaggcgtgac tgctgatgga acgattccag aagcagtggc
    32401 tgaacaagtc aacacgctat tacgtggaaa cttcaaacca gaattcttga accgaatcga
    32461 cgacacgatt ttatttacac cattgagcct agacaacgtg aaaggaatcg ttgacaaaat
    32521 ggtggcgcaa ttggcacaac gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga
    32581 agccaaaacg tggattgctg aaaatgctta tgaaccagca tatggtgcaa gaccattgaa
    32641 acgatttatc actaaggaag tagaaacacc actagctaaa gaaatcgttg caggacatgt
    32701 tatgcctaaa tcaaaagtaa cgattacttt acttgatgga caacttcatt tcaaaacaga
    32761 agagctagaa gaaatcgtct aatcttccat tgagactata aaagaaaaaa ggggtctgac
    32821 acaactagtg tcagacctcc ttttttcaaa taaacagtgt tccagaagta gtttcttcga
    32881 aaaataagcc gaaatttctt gaagctgccc gcttctgtca caacctcttt tattaggtat
    32941 ttttcacttt ttctaattta taactttcaa agtaacgctc atctttttta ctttgagacc
    33001 atgcaaacaa tcccattaag gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat
    33061 ccatcaatgc tgctcgtccg acaaacagtg tgccatcaga agcagcaaaa cgttgaagat
    33121 tcatgcctaa gaactggtcg cataagaatt cccagaactc ccaaaacacg ccgcaaagtc
    33181 cagcaaacgc aaagccgaat aagaggaaca accaaggact ggtttttgat atctcagcat
    33241 ctttcatcaa gtaaccaatc aatccatagc caagagctgt caggaccatt ggactcaccg
    33301 catgtaagat tttgtcccaa aaagagatga tactgatcaa atgcatccct gtgcctaaaa
    33361 agacagagat gaacaagaaa aaccagtaaa acaaaacgat tgctgctggt attttaagtt
    33421 tgaaaacttt caaaatgact tgtggcatga aaatcaatgc aatccctaat aaagattcca
    33481 cgataaatgt gattccttga gaagtcgaat atttttgtgt gataaattca taaaaattat
    33541 agcagacaac gaatacacca aaagcaagta aagctatttg cacctttttg atttctttaa
    33601 tcactgaaag tccctccatc taaaaatctt tttacttctg aaagcaactc aatttctgaa
    33661 gaaaccactt ttccgttcaa tttgatcaaa cctattgtat ataagttcag gtagtgaaat
    33721 tgattttcag ctgtttcttg tagtgcagcg atttttttcg ggtgggttcc gccttgctgt
    33781 cgagtatcag tatataagcc gatcactggg atgcctttcg cataagccac accgatttcc
    33841 gatgctaccc cggcatcgat cgtcagccca tctagtaaag caatcattaa atcactagcc
    33901 aacacttttt ctgtgtcagc aagagcaatc attcgactgt ctgcataagc agttttgtca
    33961 ttgattgctt cattttcttg aggaagatag atcgaaagct cagaagagag ctctctgatt
    34021 tctttaacta actgttggtt atataacaaa tcacttttag caaacattgg tgcggcaaaa
    34081 taaattttca tctaaacatc atccttttta tcaaaaataa tcgctaaagt aagcttccat
    34141 gtttggaatg atacgctcta gtgacttcag tgtttcttca tctgtttcta accgttcgat
    34201 tcttgccaaa taagaagcac ggcgataatt catcatcaag caagtcaatg tttggatatc
    34261 cagtcgaacg gattttccta taggttcatt taagacacgg acttgtccct gttcatccca
    34321 tgttaaagcg aagacaccat tattccaagg agcgacagga tcttcaatga tgaaatgaaa
    34381 agcatcagct gttccaacaa acggaaaccg ttgaaggaat tcttttacat caacgatccg
    34441 agccataaaa tatggttcga tctgctcttt gatctgacta tcttcaatca aaaatgctaa
    34501 aggctcattt ttataaatat ctccatgtac ccagtagatc atggaaaaat gtgcggaaat
    34561 aaagttccat aagccatttc gagcttcttg atcaagatag aacatttctt ttacgtgaaa
    34621 tatttcttca gctacccagt agaacagaac acctcttggt tcatggttgc tatcgtagta
    34681 gacagcggct gtgcgctcat cttcattttc gaaacgccaa tactcttccc aatgaaaatc
    34741 actgcggaat aaagcaccat ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc
    34801 tggatgatca actggtaatc gctcaaccat accacttact gggatttgtt taggtagctg
    34861 tgtatctctg attttaaagg aaagtttatc agacatgatt tcccatcctt tacgccgata
    34921 gtaggggata ctgtaaggat acaaataaga gatccattgc ttattcttgc gcatattatc
    34981 caatgcaacg ataattagat cttgcatcaa tccatggtta gcatattctg gataagttcc
    35041 aactccagtc acaccaccca ttttataacg tgcaccgtga atattgactt cacaaggata
    35101 gatagcgatt tgagagatca gtttattttc gtggaaccat ccgaacactt tcgatacttc
    35161 caaaatcggt tttttggaac ggataaaagc tcgtttattc tcaaacccgc tttcctcgat
    35221 gtctgcttca gtcacttgga acacatagga aagcaattca ttgaattgat cgatatgttc
    35281 ttgagtgacc ggttttaatt caagattttc tctaaaatct ttttgatcca ttttttcatc
    35341 tccatttatt agcttgataa cagtatagca aaattctaca aaaaaaactt ctatagctga
    35401 ctaattaaaa actctttaaa ttactgcttt tcgttggtat aatggaaagt gctgattata
    35461 tgaaaagagg agacgctatg gatataaaga aaatgaaaga acgccaagag cagattcgta
    35521 atttttcgat catcgctcat atcgaccatg ggaaatctac attggccgat cgtattttag
    35581 aaatgacgca cactgtgacg tctcgcgaaa tgcaagatca gctgttagac tcgatggatt
    35641 tggaacgtga acgtggaatc acgattaaat taaatgctgt agaactcaat tacacagcga
    35701 aaaatggaga aacatatatc tttcacttga ttgacacccc agggcacgtc gacttcacct
    35761 atgaagtctc tcgaagcctt gctgcctgtg agggtgccgt tttagtggtc gatgcagcac
    35821 aaggaatcga agcacaaaca ttggccaatg tttatttagc gttagacaat gatttggaga
    35881 tcctgccagt catcaataaa atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag
    35941 aaatcgaaga cgtaatcgga atcgatgcta gtgaagctgt attagccagt gccaaatcag
    36001 ggattgggat tgaagatatt ctcgagcaga tcgtggaata tgttcctgca ccaagtggag
    36061 acttagatgc accgctaaaa gctttgatct ttgactctat ttatgatagt taccgcggtg
    36121 ttgttctaaa tgtccggatc acggacggta tggtcaaacc cggagacaaa atcaaactga
    36181 tgagtaatgg caaaacattt gatgttacag aggtcggggt cttctcgcca aaagcagttg
    36241 ctagagactt tttgatggtc ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc
    36301 aagatacacg agtaggggat acagtaacgt tagcagacaa tcctgccgct gaagctttac
    36361 caggttaccg aaaaatgaat ccgatggttt attgtggtct ttatccaatt gatacttctc
    36421 gttacaacga tttgcgggaa gctttagaaa aactgcaatt gaatgatgct gctttacaat
    36481 tcgaaccaga aacctctcaa gcattaggtt ttgggttccg ctgtggattc ctcggacttc
    36541 ttcatatgga tgtcgtccaa gaacgcttgg aacgggaatt caatctagaa ttgatcacga
    36601 ctgcaccatc cgttatttac catgttaata aaacggatgg tacgactgct acagtagata
    36661 acccggcaga ttttcctgaa cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag
    36721 cacagatcat ggtcccaaat gattttgtcg gtgcagtaat ggaactttct caacgaaaac
    36781 ggggagaatt cattacgatg gattatttag atgattaccg agtaaatgtc gtctataata
    36841 ttccgctttc tgaaattgtc tttgactttt tcgacaaatt gaaatcaagc acgaaaggct
    36901 atgcctcatt ggattatgaa atgtctggtt atcaaaaaag caagttagtg aagatggaca
    36961 tcttgctgaa tggagaaaaa gtcgatgcat taagctttat cgttcaccga gactttgcct
    37021 atgaacgtgg aaaagcaatc gttgaaaaac tgaaaaaact aatcccacgc caacaatttg
    37081 aggtgccaat ccaagcagct attggacaaa aaatcgttgc ccgttcagac atcaaagcct
    37141 tgcgtaaaaa cgtcttggct aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct
    37201 tagagaaaca aaaagaaggg aagaagcgga tgaaacaaat cggctccgtc gaagttccgc
    37261 aagaagcctt catggccgtt ctgaaaatgg atgagcaaga tagtgtgaaa taacttataa
    37321 tcaatcgaaa aacctattaa ataaaccttt ttgagtagat gcaacactga ggattatctg
    37381 tataaaagat tttaaactgt catttggtta tcacggcgat actaaaatta acaaaattgg
    37441 aatatttttc ttgaagattt ttagctgtca gtatatgatg aaagcatctc ataggaggtg
    37501 cttttttcat ggttgataat tcaagtggaa gggttcttaa atctctaaga aaagagaaaa
    37561 aactatctca aaaaaagtta gctgatttag caggtatttc acaaagtact ttagttaaat
    37621 atgaaaaagg gtcaagaaaa atccctaaag acgtagataa tactctaagc aagattctga
    37681 atatagaaac tctaataaaa gatgaggaag ataagattga gattctcatc ggtaaactaa
    37741 ttgcatacag agatatgaat aagttactta acaaagagtt agctgataat ataggaatta
    37801 gtgaagtatt actcagttat gtgttaaata gaaagagaaa tccgtcaaaa gaaatgcaaa
    37861 aaaaaatcga tatttttcta ttaagtaatg aaaaagaaat tttgaaggag attaatcgtg
    37921 atagtgagat atttagttta tcaaaagacg ataaaattgt tatgggaaaa agaatacgag
    37981 aagtccgaaa gaaccgggaa gagacgcttg aaaaatttgg taaaaacttt accatctata
    38041 caggcaaaaa tgttataagt agatgggaaa aaggtattaa tattccagat attgaaaaac
    38101 taatgaatat tgcctatctt ggtaaggtca ctgttcctta ccttatgtac ggagaagatt
    38161 ataagaatat acttccaaaa gatgaaagag ttagcgattt taaaaagata aattctttta
    38221 gtatgggatt aaggatgagg aaaattagga aagattatta tctagaaagg gaggagtttg
    38281 gaaagctttt ttctccttca atttcaaaat ggtccataga taggtatgag aatggtagag
    38341 atatacccaa tactaatcga attatccaat atgcttatat aggaaatctt tctttagaat
    38401 ttttgattta cgggatataa aaatagttta cgtcgatact tattaagtta tcgacgtttt
    38461 ttattataga aaaaatttat aaaaggaggt ttcttgatat gataagaaac aaaaagggtt
    38521 tgttgaaagt tgctttactg gcagcttttt tctaagtaac tttttgacaa gctcaatggc
    38581 aacacaagcg gaagaagtaa tacaagaact aactgttatg aataacagct agataaaatg
    38641 ggctatgtat gttatccaac aatttagtaa tcactagaag cctagcgaaa gttcgcttga
    38701 taagttatag atgagtattg atttgtagtg tttccaaatc aaaagggtac agagccacat
    38761 gaaatatgtt gtcctctgca gcgtcaggat gggaatccat accatggaga ttcggagatc
    38821 tgtgagggaa tacttacgtg gttaatatgt cagttgtgtt ggacgtagta atggtcatag
    38881 tgggtagatt gactgaattc ttatatgaga aaatgaaacc aacaagaaga tagggttatt
    38941 tatacagaat tggggatctt ttctggaaaa gtaatcggat aatgcgagat tcgtacggtc
    39001 ttttttaaat ttctctaaaa aaaacggcat cgaatgggtt gggtgattga taagcaat
//
LOCUS       DAYYCQ010000016        38524 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000016 DAYYCQ010000000
VERSION     DAYYCQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.016"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..627)
                     /locus_tag="ACHAYF_000882"
     CDS             complement(109..627)
                     /locus_tag="ACHAYF_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8578341.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(629..1246)
                     /locus_tag="ACHAYF_000883"
     CDS             complement(629..1246)
                     /locus_tag="ACHAYF_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8578342.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1243..2097)
                     /locus_tag="ACHAYF_000884"
     CDS             complement(1243..2097)
                     /locus_tag="ACHAYF_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578343.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2094..3005)
                     /locus_tag="ACHAYF_000885"
     CDS             complement(2094..3005)
                     /locus_tag="ACHAYF_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578344.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3046..4380)
                     /locus_tag="ACHAYF_000886"
     CDS             complement(3046..4380)
                     /locus_tag="ACHAYF_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8578345.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4588..5418
                     /locus_tag="ACHAYF_000887"
     CDS             4588..5418
                     /locus_tag="ACHAYF_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8578346.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5662..5911)
                     /locus_tag="ACHAYF_000888"
                     /pseudo
     CDS             complement(5662..5911)
                     /locus_tag="ACHAYF_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6102..6296)
                     /locus_tag="ACHAYF_000889"
     CDS             complement(6102..6296)
                     /locus_tag="ACHAYF_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578347.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6801..6986)
                     /locus_tag="ACHAYF_000890"
     CDS             complement(6801..6986)
                     /locus_tag="ACHAYF_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578348.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7836..8177)
                     /locus_tag="ACHAYF_000891"
     CDS             complement(7836..8177)
                     /locus_tag="ACHAYF_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8578349.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8177..8407)
                     /locus_tag="ACHAYF_000892"
     CDS             complement(8177..8407)
                     /locus_tag="ACHAYF_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8578350.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8694..9032
                     /locus_tag="ACHAYF_000893"
     CDS             8694..9032
                     /locus_tag="ACHAYF_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578351.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9237..9799)
                     /locus_tag="ACHAYF_000894"
                     /pseudo
     CDS             complement(9237..9799)
                     /locus_tag="ACHAYF_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(10531..11112)
                     /locus_tag="ACHAYF_000895"
     CDS             complement(10531..11112)
                     /locus_tag="ACHAYF_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8578352.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11295..11921)
                     /locus_tag="ACHAYF_000896"
     CDS             complement(11295..11921)
                     /locus_tag="ACHAYF_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8578353.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11943..13274)
                     /locus_tag="ACHAYF_000897"
     CDS             complement(11943..13274)
                     /locus_tag="ACHAYF_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8578354.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13429..13830)
                     /locus_tag="ACHAYF_000898"
     CDS             complement(13429..13830)
                     /locus_tag="ACHAYF_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8578355.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13916..14122)
                     /locus_tag="ACHAYF_000899"
     CDS             complement(13916..14122)
                     /locus_tag="ACHAYF_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578356.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14217..15719)
                     /locus_tag="ACHAYF_000900"
     CDS             complement(14217..15719)
                     /locus_tag="ACHAYF_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8578357.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15737..16192)
                     /gene="argR"
                     /locus_tag="ACHAYF_000901"
     CDS             complement(15737..16192)
                     /gene="argR"
                     /locus_tag="ACHAYF_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8578358.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16290..16907)
                     /locus_tag="ACHAYF_000902"
     CDS             complement(16290..16907)
                     /locus_tag="ACHAYF_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8578359.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17128..18501
                     /locus_tag="ACHAYF_000903"
     CDS             17128..18501
                     /locus_tag="ACHAYF_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8578360.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18576..19688
                     /locus_tag="ACHAYF_000904"
     CDS             18576..19688
                     /locus_tag="ACHAYF_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8578361.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19760..21397)
                     /locus_tag="ACHAYF_000905"
     CDS             complement(19760..21397)
                     /locus_tag="ACHAYF_000905"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8578362.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21565..22173)
                     /locus_tag="ACHAYF_000906"
     CDS             complement(21565..22173)
                     /locus_tag="ACHAYF_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8578363.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(22557..23708)
                     /gene="queG"
                     /locus_tag="ACHAYF_000907"
     CDS             complement(22557..23708)
                     /gene="queG"
                     /locus_tag="ACHAYF_000907"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8578364.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(23727..24479)
                     /locus_tag="ACHAYF_000908"
     CDS             complement(23727..24479)
                     /locus_tag="ACHAYF_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578365.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(24620..25570)
                     /locus_tag="ACHAYF_000909"
     CDS             complement(24620..25570)
                     /locus_tag="ACHAYF_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8578366.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(25571..26410)
                     /locus_tag="ACHAYF_000910"
     CDS             complement(25571..26410)
                     /locus_tag="ACHAYF_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8578367.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(26407..27153)
                     /locus_tag="ACHAYF_000911"
     CDS             complement(26407..27153)
                     /locus_tag="ACHAYF_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8578368.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            27391..28761
                     /locus_tag="ACHAYF_000912"
     CDS             27391..28761
                     /locus_tag="ACHAYF_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8578369.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(28816..29712)
                     /locus_tag="ACHAYF_000913"
     CDS             complement(28816..29712)
                     /locus_tag="ACHAYF_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8578370.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(29826..29996)
                     /locus_tag="ACHAYF_000914"
     CDS             complement(29826..29996)
                     /locus_tag="ACHAYF_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578371.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(29993..30670)
                     /locus_tag="ACHAYF_000915"
     CDS             complement(29993..30670)
                     /locus_tag="ACHAYF_000915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8578372.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(30761..31666)
                     /locus_tag="ACHAYF_000916"
     CDS             complement(30761..31666)
                     /locus_tag="ACHAYF_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8578373.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            31841..33265
                     /locus_tag="ACHAYF_000917"
     CDS             31841..33265
                     /locus_tag="ACHAYF_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8578374.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            33311..34342
                     /locus_tag="ACHAYF_000918"
     CDS             33311..34342
                     /locus_tag="ACHAYF_000918"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8578375.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            34349..35536
                     /locus_tag="ACHAYF_000919"
     CDS             34349..35536
                     /locus_tag="ACHAYF_000919"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8578376.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            35692..37074
                     /locus_tag="ACHAYF_000920"
     CDS             35692..37074
                     /locus_tag="ACHAYF_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8578377.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            37111..37431
                     /locus_tag="ACHAYF_000921"
     CDS             37111..37431
                     /locus_tag="ACHAYF_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578378.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(37631..38299)
                     /locus_tag="ACHAYF_000922"
     CDS             complement(37631..38299)
                     /locus_tag="ACHAYF_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8578379.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        11198 a         7790 c         6898 g        12638 t
ORIGIN      
        1 aaacttaagt tattaagaca aatgaataaa caactacgct ttcctcgaaa caaaaaaata
       61 aagcacaaat gcgcatttac gcactcatgc tttattcgta caattaattc agataacggc
      121 agcgcatagg atgccaataa cacttcctaa aatagctcct cctaaaatgt cagtaggata
      181 atgtaaaagt aaaaaaatcc tcgaataagc gatcaaacaa gcaaataaaa aggctaatat
      241 tcctgcaact ggacttaatt cgaacaacgc agctactgat aaaaaggcta gcgctgtatg
      301 tcctgaagga aaagaagaag tcttcggttt acttgtgtaa aggacaagcc gacgattttt
      361 ttcataaggt cttttccgtc ttgtcgtttt tttgataaaa agcaaaccaa caaaaaaata
      421 gatcaaactg ccgataagaa tggctagacc atattctcta gtatcaggta tcaagactaa
      481 aacagcagca aggagcaacc aaataaaacc accattagag atattactga taaatttcaa
      541 aaaattgatt tttcgactat ccgttttccg ttgcttggcg aacttgttga taatactatc
      601 atcgatttta gcaatcaaac gcaacatgtt attcctcctt tttaagaacc ttttcttgcg
      661 tacgatggat aaaaagcagg acttgaaaat aaaacaagaa actgaaacct aataaagcaa
      721 aatagattgg tcctgcaaac cagaatgcga caagaaggat caacgtgcct gccaaataga
      781 aaagcgcgct tttgaaatca ttaaaaaaca agaaaaatgc agtaatccaa gaagcttctt
      841 tttgttccag ttgaagcaag attttgtaac taaagagaag cgcgcttaat agaagcaaaa
      901 acaacgtagt atacatcaat gtatagccaa ttcccccacc ccaatagcgg aaaatctgat
      961 aatcgatcaa tagaagaaga aaataaacac ttgctaaact atcttgcttt ccataaagag
     1021 agaagttctc acgaaacttt ttccagaata gtgaaccgag ctttttctct tcattttttt
     1081 tgtaatgata aatcatatac agaactgttc gacttgcagt tcctaatgtg aaaacaggca
     1141 agacgcacag gaaccatagt agctgtaaat ggataagacg tggaatccat tcaatgattt
     1201 ctaagaaata atcattggct tttttaccaa tcgatcgatt catcatcctt ttgaacctcc
     1261 tgtcaaatcc atcccttcaa tgaaataacg ttgtgcaaaa aagaacagca aaatcgtcgg
     1321 gatcaagaca agtgttgctc ctgccatcag ataattccat tgtgtcgttg cttggttctg
     1381 aaaaacttgc aagccaatct gtaacgtgta tttttcttga tcttggatat ataacagagg
     1441 acctaagaaa tcattccaag ctgcgttgaa ggaattgatc gcaatggtaa tcaaggcagg
     1501 tttcgtcaat ggaagcatca aatgactcca aatatacaaa tgattcgctc cgtcgatttc
     1561 agcagcttcg atcaattcat tattgatcga taagtaaaat tgacgcatca gaaatatatt
     1621 gaacgcattg ccgaagaacg atggaacgat caggggcaaa taagtaccta cccagccgat
     1681 cttggagaat aatacatatt gagggatcat cgttacaaac cccgggatca tcatcgtaga
     1741 aagaaccaaa gcaaacatca gctttttccc aggaaaatca agcttcgcaa atccataagc
     1801 aataaaagaa tttgaaagga cattaccgat cactactagc acagtgatga ataatgtatt
     1861 tcttgcatag cgccaaaaag gaaaggcggc aatcgtatcc agatagtttt cccatacgat
     1921 tttttctggg aaaaaggtcg gaggataacg aacgatttct tccattggtt tcaatgaagt
     1981 aaacaccatc caaagcaatg gaatgatcaa aatgattcct attactgtca tgaggacata
     2041 acttagtatt ttttttgttt gccgattcac actggtcttt ttgaaataat ccatcatttg
     2101 ccgtcacctc cagaataata gacccactta ggtgcaagct tcaaattgac aaacgtcaga
     2161 accaacacga tcagaaacag gatcattgac atagccatcg cataacccat atcaaaagct
     2221 acgaacgcct tgttccacat atgcagatta taaaaaagca aagaatttgc aggtccgcct
     2281 gttccatttt tcgtcatgac ataagcttct tgaaaaattt ggaaagaacc gatcgtagag
     2341 gtgatcacat cgtaaaaaat aataggagtg attaatggca aagtaatact tttgaacttt
     2401 ccccaaacgc ctgctccatc gatttctgct gattcatata aagatctagg aacattttgt
     2461 aatgtagcta agtaaagtag cattgctcct ccagaactcc atattttcat caaaatcaac
     2521 gcaggtttcg tccattgtgg atcaaacagc caagctggtc cagggatatg gaaccagccc
     2581 agaaatgtgt tgatcaatcc tgcagatgga gaaagcagct gcatccataa gatgtagact
     2641 gctactcctg ataagactgc cggtaaatag aaagctgtac ggaatacact gattcctttg
     2701 attttttgat tgagtactgt ggctaaaaac acactgaaaa tcgtcgtcag cggtacattg
     2761 aagatgacga aataaacggt gttgtataac gatttccaaa ataaatcatc gtgaacgaac
     2821 attcttttga aatttgctag accaatgaaa ttcatcttgg atgtcaaatt ataatcggtg
     2881 aaactaccaa taactgaaaa aagcataggc cctagcataa ataccaagaa cccgataata
     2941 aaaggcgaga taaataaata ggcccatctt gcttcttttt tctttcggat agataaacgc
     3001 ttcaaatttt cttgcctcct ctgtacctag acagtcaaaa atggattatt gattttgctt
     3061 gactaagttt tctactgctt tttgtgcatc tgccaatcct tcttttggtg attttgttcc
     3121 taaaagaatc tggtcaatct gttcattcac taatgtcgtg taatccggag catctaaagg
     3181 aactggtgtg atcaccgtat ttttaaagtt ttcgtccgcc atttgataga tcatttttcc
     3241 tttatccgtt aggttgtcat tattcactaa attctcattg gctgtcttgc tcgccatgat
     3301 atcaaaactt ttttcgccga atttttcttg tacttctgga gaagaaagat atttgatgaa
     3361 ttcataagag gcctcaggat cttttgctcc gtatggtact tccaagacga atccgccacc
     3421 ccacgaccaa tggcctgaac cttcttcttt ttctgggagc tgcatcacgc caaaatcaaa
     3481 atcatccggt gcattttcca tcaaactaga gtaataattg atattttggg cacgcatggc
     3541 tactaatccg gaaataaatg gatcagatac accagaccca aattcggctt ctaatttatt
     3601 gatcgtattt ttcccataat aatcttgcca gtcttggatc cactccaagg cttccacttt
     3661 atttttagta tcaatcttga ctttttcatc tttgtcaaac caactagtcc cgttatctgc
     3721 gtttagcgcc catacatcag cccctaaatt ccataatgga taaaaaccga tgcgtgtaaa
     3781 ttcttttcct tcttttttat ctagtttacg tgctgtttct tctaattcat cccaagttgt
     3841 cggtagatct tgttcactga ttccagcttc tttaaataac tttttattat aaaaaatgac
     3901 ttgtgtatct gtattgaatg ggatcgcata aggatcacct tcatattcta ctgtatccca
     3961 aagctgcgga taaaaatcct tagaagtgtc tttttctaca tatttggaga gattagttgc
     4021 ttgctgtgct tctgctcttt gggcaacgga atttatgtcc tgtacaataa catcgggtgc
     4081 atttccagct gttattgcag ataaagattt agtccagatg tcaccccatg gctgatactt
     4141 ataatttaca tggatctcgt tttgtgattg attgaaatcc tcaatgattt cttcgatagc
     4201 ttcttttcgt gcaccggatc cccaaaagga ccagaaatca atattttttc ttccatcact
     4261 tgttttttca gaagaatttg ctgcacttga actggcatcg cttgcattac ttgatccgca
     4321 tcctcctaat aaaaacaatg ctgaacttgc cagcaaccct gtaaacaata attttttcat
     4381 attttacctc caaattttaa tgtaaccgat gccggtttta atataacgat atcattttgt
     4441 tctgaaaata acaatttata aggtatgatt ctaaaaaaat tattaatgat accaatactt
     4501 tatataattc gcgagcgctt tctttgaatt ttttaatctt tcgtagcaga atgagaatgt
     4561 attaaaaata gattaggagg aattttgatg tttagacacc agaaagaatt gcagtttgaa
     4621 gcaaaaccag atcgtccgaa tccattaatc gcgaaatatc tgcaggagct gatcggcggc
     4681 caatatggcg agatgagtgt agctatgcag tacctgttcc aaggctggag ctgccgagga
     4741 gaagaaaaat ataaagatat gctgatggac attgcaacag aagaaatcgg ccacgtggaa
     4801 atgttggcga caatggttgc tcgtttatta gagggtgcgc ctgttgaaga tcaagaagca
     4861 gcgatgcaaa aagatccaac aatcggtgcc attatctctg gaatgaatcc acagcacgcg
     4921 atcgtagctg gcttaggacc tcgtcctgct gacagcgtgg gcaatccatg gagcggtgcc
     4981 tatatcattg cgagtggcaa cttgttagca gactttagag ctaatttaaa tgccgaatca
     5041 caaggaagac ttcaagtagc acgtatttac gaaatgatcg atgatcgtgg cgtaaaagat
     5101 atgctgtctg ttctccttgc aagagatagc tatcatcaaa atctatggga atgtgcagtg
     5161 aaagaattag aagaaaaaga aggttcaatc ttagtcccaa gtactttccc gcgtgacaaa
     5221 gaacgaaaag acattgctta tgacttctat aacttctcag agggagacca aagtagtgaa
     5281 ggttcttggg ctagtggcaa agcactggat ggcgaaggcg aatatcaatg gttgaaagaa
     5341 cctaagatcg aaggcgctgc accaaaatta gaaccagtaa caccaaaaat gtatgggaca
     5401 cctccattca agaaataaat atctacccgt agaaacaccc gttttttgtt ccacacgctt
     5461 tgtgagcaaa actgactaaa gtcattaatc ataaccaccc ttcaaaaggg tggttttctc
     5521 tagagctata agctcaaatc accggccagc gcctaaagac gctggctttc atattttatg
     5581 ttcaagccat attgcctttg ccagttttgc ccacctctta aaaggtttat gtactactcc
     5641 ttagccctta aaaggatcgt cttactctcg tacactcagc ttatctaacg cactatcatg
     5701 tttttcttgt tcttgaatgt actttttgat ggtcgcttca ttttgtccta cttttgtcac
     5761 atagtatcct tccacccaaa agtgtcgata cccaaattta tattttagat ttgcacaatc
     5821 tcttgatgat ctcctgaata ttggctcgat attgattata aattattttt cttctatact
     5881 ttggtgtgaa tacaatgtgg tatttacaca tctactttgt acgggctaaa ctatttccct
     5941 ttattgctcc acccgcatag caagtggttt tgtatttcaa tcgctcggcg cgctcaatta
     6001 ggtctaaaga caataaatct aaaaacccgc tgcttcactg acagaaacaa cgggttttca
     6061 gaaaaaaatg aaatgaaaaa gatctcttac ggcaatctat tttatttttt tgatttttta
     6121 taaatggaaa atgcttcaag aagtccaaga aataaagccg tataaaaaca agaacctatg
     6181 aaatctttat ttattagata ttcaacagaa atcccgataa cactagccaa caatatattt
     6241 atcagaagtg taaaataagg atgcttttta tagaatagaa ctagtgttgt tttcatagac
     6301 tctcctttct tcatatttcc aattaatcac aaattcacgg cattactaga aaaattaaag
     6361 gggcgcagaa gtggaaaaat actcttcgac tttccctaca aaccaggcca tcttgtgttt
     6421 aataactata ccaaatctaa ggtacctcta aatctaaatt ccatttgtgg cggtgacctc
     6481 caatttttaa ctcttctatc tcccatccag tatacataac cttgatactt cgaccactta
     6541 tatatcgaac acacaaatac ctatccattg caggagtact acctaactta gttgctactt
     6601 gcatctaaat aacagctgaa aagataggac ttactcttag taaagtatct gaactattaa
     6661 tttttaaact atcattttta tcctcaacta catgcacatc tgttgcaact tcactttctg
     6721 tctcatccag ataaatgatt aaggaatcgg gaattgcaat atcttcagct gacgctggaa
     6781 tagttgagct aaattagtca tcatatcttt attataaaat aaccaacagc aaaaaataca
     6841 atgaaaaaca aacttggaac taatgtatca ttaataaatg ttgaaaaatt cagcaactca
     6901 ttactgtatg attttagcag cccaaccaac atatacccta taaaagctaa taagatggtt
     6961 aggaatcctt tagaattttt actcattcat agacacttcc ttaaataact tttattatag
     7021 aaggtattgt aaataataat attgacaaaa agtgactcaa aataaaaaaa accttacatt
     7081 atactggaat gaactgtatt gatagtgtac ctaaaagctc tactgacata agatcgtgta
     7141 atcggtaaag taattttatt aattatacgg gatgtaatca attagagaat aaactaatca
     7201 taaaaggtac ttttacattc ataattgtcg ctttgagatt ctactttggt tcactatctg
     7261 acccaattgc acattttcga tcattacaag tattggtgtc gttagaacaa ctaaataaaa
     7321 actagcggta gatttttcag aatctatgct aatttctgta tggatgttcc agtgatggca
     7381 aatttctctt ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa
     7441 actatagaaa ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga
     7501 tatagtttaa aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg
     7561 tcagttgttc tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag
     7621 atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc
     7681 tcaatcattc gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatgccat
     7741 cgaccacact agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta
     7801 gcatataaaa atattataga gtttacttac aatgcttaaa acatatactg tataatctga
     7861 tcaattttag ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta
     7921 aaatcaagag attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt
     7981 aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac
     8041 attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt
     8101 tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa
     8161 attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt
     8221 ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta
     8281 acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct
     8341 ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa
     8401 caagcattat catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat
     8461 tcccctttgt aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa
     8521 tagaaataaa ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat
     8581 tgaacctatg gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt
     8641 ttgcagataa aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg
     8701 attataaaca aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca
     8761 cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag
     8821 aaacattggt tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg
     8881 tagctatttt tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat
     8941 ggcaacctct tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga
     9001 attttagatt ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa
     9061 aagactcgca gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga
     9121 ccctatagat gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga
     9181 tctgtgctga agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat
     9241 accttctcca ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata
     9301 tctgttaccc aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt
     9361 ggtgcgactg tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc
     9421 tgatggctac gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat
     9481 ttgtcttttt cttcattcca ctcattggga aaacacaacg attttgcaaa aatggttgat
     9541 gaaaggtctt tttttcttct actgtctctg cacgggcatt aatcatcaat tgtccttttt
     9601 taaatccggt aaatccccac ttcatgattt ccggagtgag gatgttttct tcctcttttc
     9661 ttaacgtcac aacaagattt gacggaaaaa cttctccagc tgctaattct gctgcctgct
     9721 tcggttgttt cgtctgggct tctcgataat aattctgaag gagctctgac tctagatcaa
     9781 aaaaataacg accacacatc ctaatccctc ccaatctttt tttctatcat acactgcaac
     9841 aagaagttcg caccaacaaa acgctttcaa tttttgtaga actaaaaaag ttgtgacaag
     9901 tgacaaactc tccagtgaat ggtattctcc tgccaactcc tcggtcacaa gtcacaatat
     9961 tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg tgactcggag tgaaacggca
    10021 ggcttacgac ctctgggtaa acggtgttca aaagccagtt tctcggtatt aagccaaatt
    10081 tatgcaaaaa tgtagaaagc tattttcaca aaatttttct taatactcga aactaagcga
    10141 ctttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    10201 aaccaaacga aaaatatgag cagctatttt tatttggctg ttcttattac ttgatgcagg
    10261 gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctaacctt cggtattagc
    10321 tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca
    10381 aagcgctgat ttcacaacct ctaatacacg gtgttccaga agtagcttct tcgaaaaata
    10441 agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    10501 gctgaccact tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag ttaacaatct
    10561 ttctttgatc aattgaaaat tctgcctaag tagtgataga tcctgtgtga ctttcaacaa
    10621 taagatactt ccctggattt cagcaatcaa cgctttggct tgacggtctg cttcttcatc
    10681 ggacagtttt tcttcagaaa gtgccaggat attttgtttc gtatacttga cccattgatt
    10741 gactaattct agtagcaaca gacggtattc ttcatccact gtactcaact caataatcaa
    10801 attacctaca ggacagccat gataagtgat ctgtgtctca tgatcagcga aaatccagtc
    10861 caacatttct tctaatgctt cttctggctg ttttccagca tttaagatgt cttcgaacaa
    10921 ttctgtctgc catacgtcca tatgcgcatg gatcaccgct agacaaactt ctttttttga
    10981 agaaaagtaa taataaaatt gaccttttcc tgtatcagaa gctgctagaa tatctcggat
    11041 tgacgtttca tgatagcctt ttttataaat aagatctttg gtcgcttcca agatcttttc
    11101 ctttgttttc aatgatattc tcctttttca tttctcatag tcctatatta tacagtaaat
    11161 tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc caaattatct gacacagccc
    11221 tcactataaa actgttctca tatagctaga tgttcattga ttcaagaact gcgaggacaa
    11281 aagcaattcc tatcttatga tagttgctct aatacttcct ctgttgtcat cacttgatct
    11341 gcaataaagc caaagttcgt caacgctgca tccattgctt cttggccagg agcaccaatt
    11401 gcatctgtcg ctaccacaac attgaatcca ctctctacca attcacgcat atgggaatcg
    11461 ctacacaagt ttccgttcat tccaccaaga attactgtgt cgattccatt ttttctcaat
    11521 tggagcgcca aatcattgct ttctggtcca aagattttat gaggagagct gatgatcgta
    11581 ttttcatcca aataaggttt caattcttcg ataaaatcgg ctcctgaacc ttcttccact
    11641 gattcatatt gactttttct agcaaacatc tgctgctcaa gcatcatatg ttctcctgca
    11701 ccgccaaatt gccatttatg gtcatgtggg taaaaataat gaggagaaat aaagagtttg
    11761 tatccttttt cttttgcttg ttttaccatt tcaatcatat tttcgatcgt attgtatttt
    11821 tctaagatat cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa ctcgacttgc
    11881 gggtcagtga gaactactgc tgtattttta ttattgaaca tggtggattc tccattcgtt
    11941 atttatttta atacatcatt tagagcttca ctcattgtcg gatgagaata gatttgatct
    12001 ctcaataacg tataaggaag ttttccattc atagctagcg aaatcaagtt gatgacttca
    12061 tgagaatctt ccgcatagat acttgcccct agaatcaagt cagtttcagg gtcgactaaa
    12121 actttgaata cacctttagg atcttctaat acttgtgctt tagggacacc gcttgtcata
    12181 tacttgaata atttgtaatt gacgccttgt gctttggctt gtttttctgt taaacctaca
    12241 ttggataaag tcggagtgat aaatacactg taaggcacag ctttccggtt attcgttgtt
    12301 cgtttattct cgcccttcaa ctgatccata atgattcgat aatcatctag agagatataa
    12361 gtgaactgca atccgccttt gacgtctcca atcgcccaaa tgttgtctgc atctgtacga
    12421 aggtattcat ccacagcgat tgctccgcga tctgttagtt taacatctgt attttctaat
    12481 ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca aaacttttga cgcttggatt
    12541 gttacttctt ggccatcttc tgtaaaggtg acggcagctg ttgattcttg gtctgctact
    12601 tgatctacag atacacctag atggaattcg attcccgcat ttgtcatgtc ttctaaaatc
    12661 atttgcgcga tatcttcatc ttcacgtgga agaaattcag aatgggcatc aagaacgaca
    12721 actttagatc cgtattcatt gaacattgat gcaaattcca agccaatata tccagccccg
    12781 atgatcacta atttttccgg taattgttcc tgatccatcg cttgcgtaga atcaatcaag
    12841 tagcgacttg tattcaatcc tttgattggt aaaatgattg gttcagaacc agtattgatg
    12901 aaaatccgtt cgccttcaac aatcactttt tcaccattat tcgtcacttc gatttgatgg
    12961 ttagaaagaa aacgtgctgt accgtccatc actgtcccag ttgcctcatc gctgatcata
    13021 tgataatttt tggcgcgaag catgcccgtc aatttttctt tccgagcaac agcctctgta
    13081 aagtcgattc ctctttctcc attgaaaatc aatgatttag aaggaataca gccgatgttg
    13141 atacatgttc cgccatacat acgtgttgac ttttcaacga ctaaaaccga ttcacctttt
    13201 gaagctaacg ccttcgctaa agtcttaccg ccctttccaa aaccgatcac aatattttga
    13261 tatttttcca tcatttttcc ctcgcttctt ttttacctac cgattggtac aaaaatatga
    13321 taaccgataa ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa aaccagacaa
    13381 aaagcggtcc tttcacgttt ttagtctggt tcgatttcct ttattcttct aaacaatcgt
    13441 tacttctaca tcgatatttc ctctagttgc tttggagtaa gggcagaatt catgcgcatc
    13501 ttctgcaatt ttttgggctt cttcttcgct gatgccatcg atatgcactt ctaatactac
    13561 accgatctga tagccgccat catctaaatt ataaagagag acacgtgctt ttactgtgga
    13621 ctctgctgtc attcctgctt ctttgatcat atgttgtacc gccccattga aacaagaagc
    13681 ataacctgct gcaaataatt gttctggatt tgttccttct gcatggattc ctggaggagt
    13741 gattttcata tgcatcgagc catttggtgc ctcgacttct ccattgcgcc cgccatggtt
    13801 gatcattgct gtttcgtatg cttttttcat tgtactgctc ctttcgattg accatttgct
    13861 acttggctat aattatagtg tatgacaagt aaggcgcttt ctacaaaaat tctgcttatt
    13921 cgaataccag aatgccgtct tctactattt ttccaaaaaa caaaaaaata ctactcgtga
    13981 acaacagcgt catatttgtt ccaaaataaa tagcaatctt ttctacataa tcttttatat
    14041 gattgatttt aagatacata tataccagaa agttgacccc ataaaccaat actgttaaaa
    14101 aaataaccat attcgttacc attttttcct ccttttgctg gtctccctac cagcactcga
    14161 tttaaccata ccactttttt tgccgtccaa gcaactagcc tttcaaaaag actggtttat
    14221 tctgctgtta atgattcaag atcagaaaaa tgtccgaata attttgctaa ttttactaac
    14281 gccaacttgt tctttccgaa ttcatcattt tctatctgta atactagtaa cggctcatgt
    14341 aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct cttctagatc atatcgaata
    14401 ccttcttcat tttccgggtc gaagttcatc cctttttctt cttccaacag tcgcttcatt
    14461 tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt taatcggatt
    14521 tcttgtactt ctacgggctg tttcaactct ttgatttgtt catctaatgt gatacctttt
    14581 ctttttaatt ctggcaaaag catcaggatc tttgcgatga catatgcacc atcatccaga
    14641 aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga tggctagctg tgtatctttc
    14701 ccaagacggt taagttctaa tgctttattg attacatttc gatagccgga aatataacga
    14761 acttggatgc ctcctttttc ttcaatgaat ctttgtagat gagaagaagt aggggaattc
    14821 gtcacgattc ttgttcctgg atgctctttt aatacgattg tacttaatac tgcgatcaaa
    14881 ttatttcgat tgatcactgt tccagaagca ctgacaactg cagaacgatc cacatctgta
    14941 tcgaaaatga tcccaagatc tgcttcattt gctaaaacag cctgcttgat actttccatc
    15001 gcttctttat tatcaggatt tgggatatga ttaggaaatc ggccatctgg ttccaaaaat
    15061 tgggaacctg ttgaatccgc acctaagcta ttcaatactt tttcagcaaa gaatccacca
    15121 gcaccatttc cggcatcaac aatgattttc caaccattta gtggtttgct ttctagtgtt
    15181 ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa aaggtctgct
    15241 ttttctacgc ttccagtttg ttcgcttttt attttttgct gctgtgatag gataaaagca
    15301 atatcttctt tttctgctcc tccttctgag gtaaagaatt ttattccatt ataataatag
    15361 ggaagatggc tagcagtcag cataaccgca gcatcgcacg agaactgttc aaattgagtg
    15421 gacataaaca tagcaggcgt agtagctaat tcgaaatcga tgacatgtat cccttgttct
    15481 ttcagacttt ggatcaacgc ctctttcagt gctggtccgc ttatcctact gtcatgacca
    15541 atcccaatcg tccatttttt atttggatcg gttgatttct tctttttctt tatccagttt
    15601 gctagtcccc agccgatctt ttttaccatg tctgtcgtca agtttacttc atatccttca
    15661 actggtaatg cgattcctcg aatatccgat ccattttgca gcgctttcat atctttcata
    15721 taaaagtcct cctcatttat tccctcattt tttccagctt ttctgcaaat cgctgtgctt
    15781 cttcttctga gcggcaaata ataatgaatg tatctacacc ggccatcgtt ccgacaactt
    15841 cagggtaagc cacttcatct agccaattcg ttaccacatc ggcaccattt cgttcagtca
    15901 atacgataac catgaactga acagactgga tacgcaaaac ctcgcgtcta attgctgtac
    15961 gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa taatgtatag cgtactattt
    16021 tgttttcatt ttgacttttt actaacttca tttctcgaat gtctcgagaa atcgttgctt
    16081 gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt gtcccaatag
    16141 gttgttctgt aatgatctgg cggatcagtg cttgtcgctg tttctttttc atattagaca
    16201 cctctcaatg ttgattatag caaaagataa gtaatatttc ttcatatttt ggtatttttt
    16261 acttttattt tttgttttca ttcaggttgc taaccttttt ctattttaac acgcaaaatc
    16321 gtcttcattt tttcaggtgc tgattttctt ggatcgctga tgtaaatctc tttgtgttct
    16381 ttagaagctc tttcatagcc ttcttttgcg atgaatgctt ctaatttctc aaacgttgct
    16441 ggttcttcgg catagggccc gacgtgtaag atttgggcac aaagaccttc ttccatcttt
    16501 ttgaatacta cttgttcttt caagtcttcc ggaagtttgc tagaacgctg aatggcttct
    16561 tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca tcaattgata agaaaaatgt
    16621 tcttttttca tcactggttc atgcaaatac ttttcttgca gtccccattg tccttccagt
    16681 ggatagacag tatacatctg ataatcagga atcagccaat tttttttagg cgacatgcgt
    16741 attgcatagc tgactgcata tagacaggct actcttcgtt taaagtcttc ctgattcggg
    16801 tcgccgatcc ctctgatgct aaagaatgat tttttaggaa cctctagtat ttgcgggctt
    16861 tttttcatac tatagctctt gtctttacgc cattcaattt tttccatttt tcttcctccg
    16921 cgaaattcca tttagttatt tattatcata cctctttttg agtgacttgg ctaatcgtgt
    16981 tgacactatt tttcagtttg tgctagaata agtgaagtaa ataaatcgta cagataacac
    17041 gtttcggcgt gtcgagagag aggcggtttc tatttttata atagaaatca ctctcttttt
    17101 ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa aacagctgct tttagtacct
    17161 gcagtaattg caacagggat gatgtctttt gcgggcgtat tgatcgagac ggccatgaat
    17221 gtaactttcc caactcttat cagacagttt gatttaacga ctgcacaagt ccaatgggta
    17281 acaacgattt atcttttgat gatttcgatc attgttccgc tgtctactta tttgaatcgc
    17341 aattttagtc tcaaaaaact ctttttagct tcaaatctgc ttttcttggc aggtataatg
    17401 atcgatttct tctctcctac cttcatggtc ctattgtttg gtcgattgct ccaaggtgca
    17461 gcgacaggta tcgctcttcc gttgatgttc catattatct tgacatttgc ccccctcaat
    17521 cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg ccattgctcc agcaattggc
    17581 cccacttatg gaggcattat gacttcacat tttacatgga atcatatctt cctgtttctt
    17641 gtgcccgttt tgtttctttc tttgattatc ggctggtttg ctattcccga gatggcagta
    17701 caaaaaagtg gttatttgga ttggataagc gttttaggaa tttgtctttt gtttagtgga
    17761 agtctaacat ttttgagtac gttgggaaca gtttccagct ggattgcttt aatcatcggc
    17821 ggtattggct ggttcctttt ttactatcga agtaaaaaag tggagcatcc actgatccga
    17881 ctgtctattc tttcaaataa aacattccgc ttgtttttga ttggcttctt agtttatcag
    17941 tttttattac ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt tcaaggaagt
    18001 agtccttttg tcgctggatt agcgatgctt cccggcgcag cggttggtgc tttgctttct
    18061 cctttttccg ggaaaatgct ggatcaatat ggtccgaaga agccaatttt tgctgggctg
    18121 attctttctt tgattggttg gctagcatta ttcctactca taggacacgc ttcagtgggt
    18181 gtattgattg cttgtcatgt gttttatatg atcggtatcg gattatctta cagcaatatg
    18241 atgaccaccg gaatgaatgc actgaatgaa gaactacaag gagacgggaa cgcaatgttc
    18301 aataccttgc agcagttttc tggcgctgtg gctacttcat tagtagcggt cattatcaat
    18361 tacgtccagc atcatacttc ccataactat gaagtgtcta ctacattagg atcaaaagca
    18421 gcattaggcg tcttgttgct gttgcttctc gtaagctttg ctcgattcgc ctattatctg
    18481 ttctttgcta aaaaagcgta gattccatat acggcaggtg ttgcatctgc cgtttttttg
    18541 tgtgaaaatg gttgtttgta aaaggaggct tttttatgga aaaaattgcc attatcggtg
    18601 gcgggatcat tggtatgacc ctagccagtt acttagatac atcaaaattc aatgttgctt
    18661 tatttgatga agggacagga caggcaacta aagctagcgc tgggattatc tccccttggt
    18721 tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa agacggcgct gcttttttcc
    18781 ctaaacttat aaaagatttc caacttgatc aaacgattta cgcacaatca gggacacttt
    18841 tgcttcgacc tgaaaaagat ttagctgaat tggctgttct tgccgagaaa cgaaaagaag
    18901 aagcgccaga aattggaaac atcgaatggc tttcacctga acagacatct caagcacttc
    18961 cgttattgaa accagcagtt tcactgcgta tcagtggtgg tggtcgtctg gatggaaaag
    19021 catatttaga acatcttggt cagcgtttag cacaaaaaca aatggagcag atccaggcaa
    19081 aagtcacttt gcataagaag agcgattctt ggctcatcaa gggaccgttt ggagaataca
    19141 cagctgatcg tgtaattttg acacctggcc cggcacttaa gcctttatta caagaattag
    19201 gctatgctgc agatatacgt ccgcaaaaag ggcagctctt agcatttcag acatcttata
    19261 agatgagtca agactggcct gttgctatgc ttgacggaga agctgatttc attcctttca
    19321 tggatggaaa gatcctttta ggtgctaccc atgaaaacga tcaaggctgg gatttggctc
    19381 ctactagcga agcttataag ggattgacta agagtacaga acagttcttg actgatccag
    19441 atcagataaa aaactggcca tacgagttcc gtgtaggtac ccgtgcttat acgtcagatt
    19501 tttccccatt tttcggcgta ctgccagatg atccaagctt actggctgct agcggtctag
    19561 gctcttctgg cttgaccaca ggtccttata tcggctatct attagccaac ttcttaaata
    19621 ctggtcattg gcctacggaa gattatcaaa aggacatctc cacctatatt acgaaaaatc
    19681 catcatagaa aaacagcttt ttcaagtaag gatcccgagc gatattgcgc atggattact
    19741 tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa attctccaaa ctttcttttg
    19801 cttcatctgg caagagatct tttcctatct gagcttcaat gaatcgtaaa cgaatatcaa
    19861 gtcgttgttt caccagattt ttataggacg tatcctcaaa agaaacagga aaatctggta
    19921 taggtaaata gtggatgtct tcaaatggac caaacgactt gaattctgcc gtttgttgca
    19981 cgattctttc aacgattgac aatgtcgttt caggtttgat ttttttatca tcgaaatatc
    20041 cagtattcag gacatagcag gctgtttgct gtttcaaaaa caactctcga aaagcttgat
    20101 agtcttccct aagaggatag acacggaaag gattagcaaa tggttcaatg actagctcat
    20161 ctagctgtct ttttgtagtg atattttccg cgtctgatcg ttttgttgct aatgttgcac
    20221 caaaagacgc tgctaaaact gggtcattga tcttgataat tggcggaagc gaatcatctt
    20281 tcatgatcca aaaaacagca tcgatctttt cgctaagatg atcgactcgg tcgggtgtcg
    20341 cataacgtga tttgactgtc cgcccatttc cgtttcgtat atctcctgtt actaggactt
    20401 tttctccttt acttgtacat gtcacgccca cattctgaca agtaaggaaa gaagaaatgg
    20461 ccggatcatc agacggatag tcttgtgttt tatcaaagta agaaggttcc aaggcagtag
    20521 caaaaccatt ttttcgagaa ataacgaaag catcgtcatg caagatcgaa acagagtgac
    20581 cttccgtatg ttttgctaag gtaatcgttg attttccaga accggacaac ccaaatacag
    20641 ccatcgtata ggtctggtca ttactagcat attgtttcat cccaccgtgg caggcaataa
    20701 agccattgcg atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt aattctccaa
    20761 agtaacgtaa tcctagcaca gccgctgtat tatggagagc atcaaacaaa atgagtccat
    20821 ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat ataaagatct ccctcatcaa
    20881 aggaaacaga cttttgataa gtctctaaga tcttcgaatt cgctggctga aaattcagca
    20941 aataagaata caaattgttc tcaaatcctt ctggaatcag caaatgactt tgcacagaaa
    21001 attcttcaga taggccaacg attacttttg tatggtaaaa ctggcgcttg cgactttgat
    21061 acacagcttc tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag tcctcaaaga
    21121 tccgccgagc acttgctgtt cttcccacaa tgcttccatc attgaatact agtgtcctaa
    21181 catcagctgg taaatctagt tcttcagcat gtgagattgt taaatctgtg atgatcgttc
    21241 ctggactccc ttttgctagt tgataagctg tcttcctatc aaaaacttca tgaacattat
    21301 taccgtaaaa tgtcgtttct acaatcgatc gaatactgga taacagtgga tttccacttg
    21361 tgatttcgtt taattcgaga ctctcttttg tactcatggc tcctcctcat tccctaaaca
    21421 aaaaaacgtt ttctttatta tacattattc gagtttttat ttgtactgct gcgctgtggg
    21481 aagcaaacga gttatttttc acacaaaaaa accgagaact tgaataaaca agtttctcgg
    21541 tttcatcatt ttccttttat cggcttagta ccagccgttt gctaaccaga atgttttagc
    21601 agcatcccat gaaccataac gagatgttac atattgttct gctacacgtt cttgattggc
    21661 agctgaatag tcgccgttta agtatgaagc atctaattga taacggccga tgtaacgtcc
    21721 gttagtagct gtgtatgaac cgcctgattc tttttgtgcg atccattctt ttgcagagct
    21781 tgaagttggc gctgttgcag cagctggggc agcaggagct acttcaactg tttcaacagg
    21841 tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta gatgctgtat tttctgttgt
    21901 tttgttttct gttgttgctg aagaatcatt tgaagttggg attgttaatt gttcaccaac
    21961 ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca attgcatcga tcattgagtt
    22021 gtctcctgca aatttttgtg agatcttaga taatgaatca cctgatttta ctgtataaac
    22081 ttcatcagcg tgagcatttg tagttcctac aaacattgca gtagcaccag cagcaaaagt
    22141 tgtacctaaa acaattttct taaatgaatt catattaaac tccttttttt ctccgttttt
    22201 tattctttcg acgctattaa ctttatcatt tagaaatatt tcataggtaa aagaaacatg
    22261 acatttaatg acaaaataac gaattattta atatttgtaa catttaaaca aatatttttt
    22321 tgatacaaaa ccataaaagc cttgttgtat aagcaaagta atcgtttttc tatgtttttt
    22381 tatttttgaa tgaaaaacgc agattctgta acattcactg caataatcca taaaaaaaag
    22441 caaaaaaatt tttgattttt atcaaattct cttaaaaaaa tgtaaaaaat acaaaaaaac
    22501 cagtaattgt tacaacaatt actggacaaa aactttgaat aatgttaact atttttttat
    22561 tttaactttc tccgcaataa tttctgggca tgttccaatt cgtttataac gacttcttct
    22621 gtttctttag ctaatatttc atctaataca tcaagccatt tttccggttc ttttgttcct
    22681 agacgtccaa tagcccaagc tgctgttcct ctgatgacag ggcggacatc tgttaaacat
    22741 tctgaaatct caggcaatgc cgttcgatca cctagattag ctaaagcaat caatgcgttt
    22801 ctctgcaaag gtttcttccc acgccatgaa ccagctaaat gcccaaattg ctgtttgaag
    22861 tctttattag acatcgaaag cattggcttt aattttggat atatttcgtc tacttttggt
    22921 tccatctctt catgaaaatg aaaatctttt ccttgattgt aagggcagac taactggcag
    22981 atatcacatc cataaatgac atttcgcatt tttttacgat attcttccgg catcatccct
    23041 tttgtctgag tttgataaga caaacatcgc tgggcattca ttcttccgtc accaagcaat
    23101 gcttttgtcg gacaagcagt cacgcagcgc atacagtcac cgcaaccaaa ttctcctggt
    23161 ttatcaggct caaatacaat attagtaacg atctcaccaa gatagacaaa tgaaccgaac
    23221 tcttcagtga tcaacaatcc gtttcgtcca ataaatccta aaccagctcg ctgtgctaca
    23281 gcgacatcga tcagttctcc agtatctact tgcggagcta gacgccattc ctcttctttt
    23341 tgccattttt ctgctcttgt ttggatgaat tcgatcaatc ggttcaaacg gtctctgagt
    23401 atatcatggt aatcgatccc ccaagaagca cgagcaaact gcccgcgttt ttcatcccta
    23461 ggcacttctt catggatcct agtaggatac gcaagtgcaa tggaaatgat cgatttcggc
    23521 tgatcaaatg tcatttctgg ataaagacgc tcatcgatat ttggatgttc aaagccagaa
    23581 gtatggccag ctgctttttg ttcttccaac gattctttta atgaatcaaa aggttccgcc
    23641 gtggtgaacc cgattttgtc aattcctaat cgtttgcttt cttgtatgat ttcttctttt
    23701 aatgtcatgg tcttcctcgc ttgctgttaa cggctgtata acccataata tagtttttct
    23761 tttttcagct ggacaaactg gatctgctga tcggcaatct tcttttcttc catccatttt
    23821 tttcttgcag caaatacttc ccaataattt gttgaaatta attttctttt gttatctaat
    23881 gcattaaaaa aattcaatga aactggtttt gtacttaaca gttcatggag ccttacctct
    23941 tcccatggcg aaaactcaga cagatcgcgg gaatacaata cttcttcata gagatttttt
    24001 tgctgtgtgt acgtgaatgt ttgcgggatt ttcccaggcc tagcaatttc tactagacga
    24061 ttataaaaca tattcattaa tttgctgttg gcaataaatt gacttttata ataggccgac
    24121 tggatctttt tgattactcc ttctgctttg atgactgaaa ataatgcagg ccatgctttg
    24181 acggttattt cgtcgttttg tttaactaat acgccaggtg tgaagccgac tggagaaagg
    24241 aacacaaaat aaccgatacc ggtattttca tctaaaggct gaaattcatc aaacaaccat
    24301 ttgcctatgt acagctcttc atctggttct gttgtctcat tttgatttgc tatgaataat
    24361 ttagctaatt ctgcttcaag aaattgaagg tcttgatcct tgtcggtatt ttcctgctgg
    24421 ctgatcaatc gaaagacttc ttctttaatc gtatatttat ctttacgtgt aaaattcata
    24481 gccacacctt ccttccttac tctaactata acggaatttc ttcaaaaaag gtatctgtac
    24541 gacgaaaaac agcataattt tttttcatat ctcttataaa aaatgcaagg tctgctcctc
    24601 aagctagacc ttgcactttt cagctttctt ttgtttgtga caaaccttca tggatcgttt
    24661 caatattcca tttcatcatc tgatagtaag aatctccttg ttctccttct tttgctatgg
    24721 aatcagtgaa cagtttatca tagattttca aacccgtttc ttttgctacc cgttccatgc
    24781 ttcttgagtc tacactggtt tccacgaata aaaccggtac ttcttttgct cttatctgat
    24841 caatgatcgc tttcatttga tcaggcgtac cttgactttc tgtattgatc tcccatatat
    24901 aagctgctgg caaatcatat gctcgtgaaa aatacttgaa tgccccttca cttgttacta
    24961 ataatttttt gttctcttcg atagaagcaa aactttcctt cgcttgtgta tctaattctt
    25021 ttagtttttc cacatatgct tctgcatttt ttttatagag tgtcgcattt tctgcatctt
    25081 tttcagagaa tatacgtgcg atttcctcca catattttat tccgttagat aggtctagcc
    25141 aagcatgggg atctgctttt gtatgttctt caccgctagt taaataaaga ggttctacat
    25201 ttttgctaac tgcaaaataa tcttttcctt cttctttttt agccgtttcc atcaagttat
    25261 cgaaccagct gttacctgtt tcaagattca aaccgttgta taaaataaca tctgcatcgc
    25321 ttgccttttt gatgtcttct ggtagtactt catattcatg cggatctgtt ccgactggta
    25381 cgatactgtg gatatctatt tgatctgttc cgacctcttt tgccatgtcc gccaagatcg
    25441 aattggtagc cacgactgac aacttttctt tacttccttc atccgcttgg tcagaagcct
    25501 gctttccgca accggctaat accaagaaac taactgccaa gattaaaaag agtgattttt
    25561 ttgttttcat ctatctgctt cctttctgaa ataaaattcc ttttgttgga gaaaataaaa
    25621 aagcaaggat aaaaaatgcg gtacatgcta acacgatcgt agctcctgat ggccaattgt
    25681 aactataact gaaaaacaca ccgacaactg cacttagcat accgattcct gcagctgtaa
    25741 tgatcatttt cagtaaatgg ttcgttagca agtaagccgt ggcggctggt gtgatcaaca
    25801 tggcaatcac caagattgtt cctactgtct gtaaactgac tacagccact aatgtcaaaa
    25861 agaacatcaa aagataatgg atcagcgatg tgttcagccc ataagctttt gccattgtcg
    25921 gatcgaatga tgtgatcttc aattctttat aaaagaaaaa gacaaatatc aagacgattc
    25981 cactaacgag tgctgtcagc aacaaatcgc tttccctaac agctaagaca ttcccaaata
    26041 aaatatgata caaatctgtc gcactctggg catatgaaat caagataatc cctaatgcaa
    26101 aaaaggagct aaagacaatt ccaatcgctg tatcattttt caatcggctc cgctgagtca
    26161 cgaaaccaat cactgctgct gttagcatcc caaaagcagt ggctccaaag atgtaactaa
    26221 aaccaaacat ataagaaatg gctacacctg gaagaactgc gtgagagata gcgtctccca
    26281 tcaaagacat ccctcgtaaa ataataaaag aaccgatgac tccagaagca agtcctacaa
    26341 gcatggaagt gaccaaagcg tattgcagga attgataatt cattaatcca tcaataaagg
    26401 attggatcat tttttctctc ctccttttac taaatgtctg atgatctcac cataggccaa
    26461 ttgaaggttc ttcgatgtaa agacttcttg tactggaccg gaagcgatca gctgtttatt
    26521 taaaagaata acctcatcga aatattcttc tactttatga agatcatgat ggacgatcaa
    26581 aatcgttttt cctgaattct tcaattcctg caagatgtca aagatctttc gttcacttaa
    26641 cgcatcaatc cctacgaatg gttcatctaa aaagatccat tctgctcctt gggctagagc
    26701 tcttgcaata aaaactctct gaagctgtcc acccgatagt tcgctgatct gtcttttggc
    26761 atattcttcc atccctactt ttttcaaagc ttgtcttgca cgttcttttt cttttttccc
    26821 aggtctttgt ccaattcgta aagatggata tgttcctaaa agtacaacgc ctaatacatc
    26881 aattggaaag gaaagatcca attcacttcg ctgttccacg taggctattt ttttacggac
    26941 tgagtttacc gactgtccag aaaaggtgac tttaccagta gtagctggta tcaactctag
    27001 caaagatttc ataaatgttg attttcctgc accgtttggt ccgataatac cggtaatttt
    27061 ttgtggattt atcgtcaagt tgatagtttt caaaacagtt ttcccttgat aagcggctga
    27121 aaggtcttga attacaatgg ccgtacgttc caaatcttac actccttttt aggtttacct
    27181 aactttttat ttaaatatag tttaactaaa atggaaagca ttttcaagta gaatgatgaa
    27241 aaagtttttt tcgaattttc ctcgcatttt atgaaaattt tctgtagaat cacatcaaga
    27301 gttcaataag gctaacctaa taatgaaaaa catgtgttga aacgtttgat tattttttct
    27361 tgttgactca acaacgaaag gagtttttgt atggaagaag aaaagttaac tcgaagtctt
    27421 agtgcccgtc atatccaaat gatcgcacta ggtggaacga tcggtgtcgg tctgtttatg
    27481 ggtgcctcct caaccatacg ttggacaggg ccctcagtga tgggagccta tgcgattgca
    27541 ggattctttt tgtatttgat tatgcgtgct ttaggcgaaa tgctttacgt tgatccttcg
    27601 actggttctt ttgcaaatta cgcgagcgag tacatccatc ctgtggctgg ctacttgacc
    27661 gcttggagta atatttttca atatattgtc gtaggaataa gtgaagtgat tgctgtagga
    27721 tcttatatga attattggtg gcctgatttg ccggcaatta ttcccggaat cattgtcgtc
    27781 ttgtttctta tgctggcaaa tcttatttct gtcaaggcgt ttggcgaatt agaattttgg
    27841 ttttcgatca tcaaagtcat aacgatcatt ttgatgatca ttgcaggtct tggggtaatc
    27901 ttatttggat ttggtaatca tggtcaggca gtagggattt caaatctttg gaaaaatggt
    27961 ggattcttca ctggaggcgt caaaggattt ttctttgcat tatcgatcgt tgttgcttcc
    28021 tatcaaggaa tcgaactgat cggaatgact gctggtgaag cagagaatcc taaaaagacg
    28081 atcattgaag ctgtccagtc cactatcggt cgaatcttga ttttttatat tggtgctatt
    28141 tttatcattg tgagtatcta tccgtggaat caattgagtg agattggttc accttttgta
    28201 caaacttttt ctaaagtcgg cattacattt gctgcaggac tgattaattt tgtcgtgatt
    28261 acagcggcat tatcaggttg caattcaggg attttcagcg caagtcgtat ggtctataca
    28321 cttgccataa atggaaagat gtctaaaaaa tttctgaaac tatctcgtca cggtgcgcct
    28381 ttttatcctg tcgtagcaat ctcaggtgga atcttaattg gtctgatctt aaattatctt
    28441 ctaccaatgt tcatcaacca gtcagaaaat attttcgttt tcgtatacag ctcaagtatt
    28501 ttgcctggta tggttccttg ggtagtgatt ttgatcagcg agattcgctt caaaaagaag
    28561 cacgcagata aaatgcatga tcatcctttt aaaatgccac tatctcctta tacgaattat
    28621 gtgacacttg cttttttagc tatcgtattg attttcatgt ttatcaatcc tgaaacgaga
    28681 atctcattga ttattggact cgttttcttg ctttacatga cgatccatta ctttgtacgc
    28741 gaaaaaagaa ccggaaaata aaaaaagaac gaagcttcac cttagtggaa gcttcgttct
    28801 tttttattgc ttttatcaat tcaattttag cgtctcaccg ggagtcagga aataattctc
    28861 catgctcatc ccattcagcg acaagatcgt ttctactggt acgccagttc gttcggctat
    28921 ttgatttgga ccttctcctt ccaaaactac ggttgtcgca gcctgtgaag tcgcgctgtt
    28981 ggtattagtt tcagaggaat ctgttgtgtc ttgtgttgtt cgagcttgca tttcagaagc
    29041 ttctaccgac tctgctgtct cagaatcaga agatgttgtg tcttgtgtgc ttgttgactc
    29101 tgcttgaaga gaggcgctag tctgcaaata aatatcggaa atcttagcga catgattcgt
    29161 ataattagcc gataattgat tgatcaaagt atctaagtct acaccgtctt tattttctgc
    29221 tgcctgatac gctgcacgta attttctgat cccttcgtcg gagattgctt gaagctcacg
    29281 tgctttttgg ttagcaactg ctgataaacc agctattccc tcttgatttc cctgaatttc
    29341 tgtctggtat tcttcaatca atgcaggcgt cttttcttcg atctttttcg aataatcatt
    29401 tacgattgct tgaagttgct ctggcaaacc atttgctgtt ttttcttcag aagtttgtgt
    29461 ctcttttgtt ttagttgatg actcacttgt ttgtttcgtc tctgttttct ttgtcgttgt
    29521 ttccgttatg gtcgtacttt tcgcttctga cgaatttttt ccatgaaaat atagacccgc
    29581 aactcctcct gctatgatca aaaacacggc aactgctgcc ccaatcatga tcatccgctt
    29641 acgagctgcc tgtttttgct gtcttcttcg ggtcatccga acattttctc cccactcatt
    29701 ttcatcatac atttttttca ccaccaattt ttatgtataa tagcgttatc aaacgcttca
    29761 tattcttatt gtagcaaata attgtcgctg tgcacatttt tatctttgtt atttataaga
    29821 aaatttcacc agttgagatg attccaagat tgtccgtcga tttctccttg cagctgcatt
    29881 gttcgttcgt ctatctcagc catcagtttc tttatttcat ataagagcgc acggtcttga
    29941 tattctgatt tttcgcttaa ttcatctaat tgttggatca gccaatcatt tgtcataatg
    30001 aagttccttt catttcttgc agtgtaaagg tcaagtcttc gatctgctct tttgcctcag
    30061 tcagtgcttc tacgatatat gcttgttttt cagattcttc ttcctgcatt ttctgatttt
    30121 cctgtactct ttgaataaac cagtcaatcc gctgagtcag cgatggtttg ccttccaatg
    30181 agagatactg ctgataagca attagcttct cccaacgttt ttcctgggat aaatacataa
    30241 attgtgctaa aacgaatggc ggaagatagg tcatatggaa tttatttgcg attcgctgat
    30301 acggcctcaa aaattcggaa atcgtatatt cttcttttcc tccagcttgg tattcttttt
    30361 ccgccacacc tgttgtcaat acgattccta gttctttccc ttttaataca tgtgctgctc
    30421 gttccaagac attatcttgc caaattttta aatgagcagg agaactatac cagtaaaacg
    30481 gaaactggaa aatgatccga tcatgctggg ccaagagctg tagttcagtc tctatatcta
    30541 tttttccgtc tggataactt gattccaaat gatgccaagt agctggtaaa ccagcagaag
    30601 cttctttgaa aaattgctga tttaaagatt cttcgatcgt tggatggcta atgatgataa
    30661 gtgtacgcat cccttttcct cctttttagt cttcgcttta cagtgtagca taaccgccac
    30721 aagtttcttt tgccgatgct tgtcatttta accagactaa tcaggttgtc caagaaactg
    30781 cggtttgttt ttccaactgt tttttcaagg tatccaaagc aatattctta aaattttcgt
    30841 ttttcatacg tttacgatag ttcgtagccg tttctccggt actctttttg aacattcggc
    30901 taaatacgac tagactattg aaccctactt gataggcaat actttccacg gattcatccg
    30961 tcagagaaag aagaaacgcc cctcgattga tccgtacccc gatcaaatat tccttgatac
    31021 tggtacccat atgatttttg aatactcgcg acaaatggct ccgattgacc gctaaatgct
    31081 gggcgatttc ctcaacagac aatttttcag cataatgtgc ctcgatgtat ttgactgttt
    31141 caacagctaa agaggaatga accttttgta caccaccgct gacctttccg ccatctttta
    31201 aaaggactga taaaaaacgg taggttaatg ctgttaactg taattcattt tccaaggttt
    31261 cttccgtatc tttcatgcat tctaaaataa tgtcgatata tttttgtatg tcagaagaga
    31321 ccatcgtgta ttggtcttct ctgaaaagcg actgccgaat gatttcttct gcagcactcc
    31381 caccaaatga aagccagcaa tacatccacg gatcttctcc atctgataag taaaacgtcg
    31441 agtctcctgg acgaatcaag aacagatcac cttttttcaa ttggaattgt tgatttttca
    31501 catgataaga tcctttgcct tccatcacga catgcaggat gtaatcattg cgaatagcag
    31561 gaccaaaaga atgaaacggc atcgttttag agaaaccaca aaaatcaaaa tacaattctt
    31621 tttctacttg ttgatgttcc ttttttaaaa aaatcgattg ctccacgtct atccctcctt
    31681 actcctttag tataatgcaa caaaaagtta aataaaagtc acagaaatat attaaaaagg
    31741 aagcgatttc ttgttaagtt taagtcatta aaagatcttt gttattcgtt gtaaccggtt
    31801 tacttagtat tatatattga acaggagagg aatacagatt atgaaaagca aaatgatcca
    31861 acgtgtgtcc tacgctttag gcgcttttgg acacgacaca ttctatgtta cattaagcac
    31921 ttattttatg gtattcgtga caagccagat gttcgcagga acagataagg caacgaatgc
    31981 aaaaatgatt gctacagtta caagcttagt tgtaggtatc cgcttagttg aaattatttt
    32041 tgatcctttg attggtggca tcatcgataa tactcgtaca aaatacggta aattcaaacc
    32101 atggttagtt atcggcggcg ttgttagttc cgtactcttg gtagcaatct ttaccaattt
    32161 ctttggctta gctactcata atacgacttt atttatcata ttatttgcaa tcgttttcat
    32221 cttattggat agtttttatt catttaagga tatcgctttt tggtcaatga ttccagcttt
    32281 gagtacggaa acaaaggaac gtgaaaaatt tgctacgttt gctcgtttcg gctcttctct
    32341 aggtgcaaat ggtactactc tagtcgttgt ccctatcgtt actttcttta cctatcttgt
    32401 tactcataaa catattgaag gcgcttcagg ctggttctgg ttcggtgtga ttgctgctgt
    32461 tatttcaggc ggtactgcga ttatcactgc cttgggcaca aaagaagtcg atactgcgat
    32521 ccgtcagcaa aatgaaaaag ctgggatcaa agatgtgttc aaagcagttg ctcaaaatga
    32581 tcagttgatg tggcttgccc ttgcttatat cgcttatgcg attgccaatg cagcaacaac
    32641 tgccgtatta ttctacttct tcaaatatgt acttggccaa ccaactgaat tttggatcgt
    32701 tggtctagtt gctgccttac ttggtttagt agctgttcca ctattcccaa tgcttacacg
    32761 cgtcatctct cgtcgctacg tttaccttgg agggatcgca atgatgattt cttcttactt
    32821 tttctttacg atcgcaggtt caagtttgcc aatcgttatc atcgggctag tattgttcta
    32881 cttccctcaa caattgattt tcttatccgc tttgatgacg atcacagact ctgttgaata
    32941 tggtcaatgg aaaaatggta tccgtaacga agctgttact ctatcaattc gccctttact
    33001 agataaaatc gctggtgctt tgtctaacgg gatcgtaggg tttgtcgcta tcgcttctgg
    33061 gatgactggt aatgctactg ctgctgatat cactgctcat ggcgtaacga ctttcaaagc
    33121 ttatgcattc tatgctcctg ctgctttgat gattttctcc gctattattt ttgcttggaa
    33181 gattacattg acagagaaaa aacatgcaca aatcgttgag aaattggaaa acaaattggc
    33241 tccagctgat acaatggaag attaacttca cgatgcagta gttccttccc attaagaaag
    33301 gatcttgaaa atgaagatta cagaaaaaga ttttggccaa ggttatcact tgataacctt
    33361 ggagaacaaa aacaagctag cactttctat ctctgattta ggtgctagaa tcgtcagttt
    33421 aaaatcaaat gatcgagaat tagttttggg atttgacacc gctgaagaat atatcgaaaa
    33481 agatccgtat atcggtgctt ctatcggtcg caccgcaggg agaatcgaaa atggccgctt
    33541 ttctctcaat ggaaaaactt accaattagc cacagatcca aaaactggac ataatctgca
    33601 tggtggtgct cctggatttg aattaaaaaa atggtcttat gtcattttga atggcgaaaa
    33661 tgaagcttct gtcattttta ctactacaag tcccgacgga gaacatggtt ttccaggtac
    33721 aatggatgtg gaaatccgct atactttgac aaaagataat atctggcgag tgacctctcg
    33781 cggcaccagt gaccaagata ctttattcaa tccgacaaat catgtttact tcaacttgac
    33841 gggagatgct tctcagtcaa tcgatcaaca tgagctttgg ctcaatagcg aagcttatgc
    33901 tcctttgcga acagatagta ttcctatagg ggtaaaagaa aatgctgcag gaagtgcttt
    33961 cgattttcaa atacctaaaa aattagcgtc cgtttttgct agtgatttgg atcaaaaaaa
    34021 tcttgttgac ggaatcgatc atccattttt cttaaaagaa accggcttgg gtaaagaagc
    34081 cgctcgcttg actagccccg ataaaagaat ccaagtagat attgctacag atgcttctag
    34141 tgtggttatt ttcactgcaa atttcggcac agaaacacca gaaatgcgca accgaaaact
    34201 ggcacatcac ggtggcatca catttgagac tcaaacagct ccaggtgcag agcgcttctc
    34261 agcatttggt tcgattcatt taaaagccgg atcagtattt gaaacagtaa cagaattcaa
    34321 aatcaaaaca agaaaggaat aactcatcat ggaaatcaaa agaagcttaa acacaaaatt
    34381 tgccgaaaca ttcggaccta aaaaaactac acaatacttc tcaccaggac gcatcaattt
    34441 gattggtgaa cacacagatt acaatggagg ccatgtcttc cccgcttcca tcacttatgg
    34501 aacgcaaggt gtagcagcac ctcgagacga taataaaata ttagtttatt ccacaaactt
    34561 tgacgatatc ggtgtcatta gttttacttt agatgagcta gaatatgata aaaaagcaaa
    34621 ctgggcaaat tacgtaaaag gaatggtcct taaattaaaa gaagctggac actcaatcga
    34681 tcatgggttt gagttattag tggaaggaac aatccctaat ggtgcaggac tttccagtag
    34741 cgcttcatta gaattattag taggtgttgt attagaagat ctgtttgatt taaaaacaga
    34801 tcgcttggaa ctagtccaaa caggtaaaaa agtagaaaat gaattcatcg gtgtcaattc
    34861 aggtatcatg gatcagtttg ccatcggctt cggtgagcta gatcatgcaa tcttattaga
    34921 tacaaatacg ctgaaatacg aaatggttcc agtgaaacta gatggttatg caatcgtcat
    34981 catgaatacg aataagcggc gggaattggc agattcaaaa tacaatgaac gccgtagcga
    35041 atgcgaagaa gccttggcac gtttgcaaaa aactttgcct atctcttctt taggagactt
    35101 agatgaagaa gaatttgaat caactattga ccaaatcggc gatgatacat tgatacgcag
    35161 agctcgccat gccgtttctg aaaatcagcg gactttgaag gcaaaagctg aattagaagc
    35221 tgggaactta gaagcatttg gtcaactgtt aaatgattct caccactctc ttcgctatga
    35281 ttatgaagta actgggatcg aattagatac actagtagat gctgctcaaa aacaagaagg
    35341 tgtcttaggt gcacgaatga caggagctgg atttggtggc tgtgcgattg ctcttgtcaa
    35401 agaagaaaat atacctgcct tcaaaaacaa tgtctatgat gaatacatga aagttgtcgg
    35461 ctatgctccg gaattttatg ttgcacacat tggaaatggg acaacaaaga tcgatccaga
    35521 agctgttcat gtttgataaa aaaatcaacg atctcagcta aaaacaaata gataaaaata
    35581 acctctcagc aatttccaaa tgtgagcctt ttgctcgtat ttgaaaattg ctgtttttta
    35641 ttgacacgga aagagaacgt gtgttctaat tgtgttgaag aggtgattgt tatgtgggcg
    35701 aatcaaaatc cggtatttgt ttatgaaaaa gaaccttcaa gagatatttt atgtattgac
    35761 tgcaaatctt tttacgcctc cgtagagtgc gttgaaaagg atcttgatcc ccttacaact
    35821 aaattagtag tcatgtccta tccgtccgat tcgacagaaa cacggggtag cggattaatc
    35881 ttggcttcca gtcctgcagc aaaaaaagct tacggcatca ccaatatcag ccgtgccaga
    35941 gatcttccct tcccctatcc aagtgattta tatattgttg ccccaaggat ggcctattat
    36001 atggaaaaga acagagaaat caattctatc tataaaaaat atgcagacga agcgaaccat
    36061 catgtctatt ccgtcgatga aagcttcctt gatgtaacaa atagcatggc actctttggt
    36121 aaaacaaatg cgtacgacat ggctaaactg atccataaag atgtgtttga gcagaccgga
    36181 atctacacga ctataggaat tggcgataac ccgctacttg ctaaactagc cttagacaat
    36241 ggctcaaaag acggaaagca aatgtttgct gaatggcgtt atgaagatgt accagaaaag
    36301 ttatggaaaa tctcagatat cactgacttt tggggaatcg gcaaacgaac agcaaaacgt
    36361 ctgaaacaaa tgggtataca ttctgtttat gatttagctc acgcggatta ttatcacttg
    36421 aaaagcaagc taggggtcat cggtactcag ctttatgccc atgcttgggg catcgatcga
    36481 agttttttgg gacaagaata tacaccaaaa tcaaaaagta tcggaaatag tcaaatccta
    36541 gatagagatt atacaagacg agaagaaatc gagatcgtta tcaaagaaat ggccgaccaa
    36601 gtcggcactc gcttgagacg atctgcagca aaaacacaag tagtcagctt atgggtagga
    36661 tactctttag gctatcttga tccaaatggc aagaccgggt ttcaccagca aatgaaaatc
    36721 gaagcgacaa atgctagcag agatcttgca gatgctttat tgaccatttt ccaccagcat
    36781 taccactacc aggatattcg gcgtgtaggc gtgaactgtt ccgacctcgt atacgcgaca
    36841 ggtttacagc ttaacctttt tgaagatcct gaaaagcaga taaatgaagc gaaagttgat
    36901 tttgttgtcg atacgattag aaaaaaatac ggctttcaat caatcatcca tgcgcatagt
    36961 ctcttgcaag gaggacgcgc catcgcacgt agttctctcg ttggcgggca tgcaggtggt
    37021 atgagtggat tagaaggtgg aaaagatgag acggacagca aaaaaattca ttgactataa
    37081 cgagtatcga gatcggccat ttggattaaa atgggggact gcttttgcga tggaagaact
    37141 agtcaacggt atcaaaaaaa tgaagaagaa gcacgcaaac agcctcttcc taaacaacaa
    37201 atgacattgc aagaaatcga tagtatactg acagaagcct ttttatctcg taaagcagta
    37261 atcattcagc tcaatttaaa agatgaattt ggaagactct tggatgaggt cgaagggcac
    37321 ttcattggtg aagcctatga agattacttt atgattgacc atcagccgat attgtgggaa
    37381 gacgtacggt atattgagtt aaaggaaaca aaaaaatggt ttgtgacatg agaggttgtg
    37441 acagaagtga tcaacttcaa aaaataagaa aggatttttc gaaaattgct tcttaaattt
    37501 ttgtggaatt ttggcttatt tttcgaagaa gcaacttctg gaacaacgta tattagatat
    37561 cagaggttgt gaaaaagtcg cttagttttg agtaacaagc caaggcgctg atttcacaac
    37621 ctctgtctat ctatccatct attcggtaag attcatgcat gacgtcttct ttatttagta
    37681 tgtagagccc tttttcttct gaccattcaa cataaatcag ctgttttaca tctgcatagc
    37741 cttctttctt caagttctcc ataagccatg cttcagtcaa ttgattggac tgaagcactt
    37801 tatgctgaat ttgcccttca tctacaagca ggatcgataa agctttgtct tcatctgatt
    37861 ttttcaatac actcatttga ccggcattct ctaggataac atgtttggca ttttcaagaa
    37921 aaaagcattc ttgctgtctt aagattgctc gcaactgctc catctcgata tggttatttg
    37981 tcaactctgt catgttgatg acgccgtctt tgattagaac agatggctcg cctttgaccc
    38041 aacgattgac cttttcattt ttttggacga tacgttcgat accataaatc attactgccc
    38101 aaagcgcaat ggcaaatagg acatgcccta catggacatt gtcatcatag gttgattctt
    38161 ctaagatccc acctaaaaca agcgtataga ctaaatcaaa cggtgtgatt tctgacatgg
    38221 atttcttccc aagcaaacgt accactaata ataaaccgat caacccgaac accagtttca
    38281 ttgtaattga aaaaatcatt ttttctcccc ctaactatct gttaattaaa ttatatatgc
    38341 tttctcacga acacgcatgg aatatacaag gaagaaatgt atccttttac taaaaaaagg
    38401 actgtgacac tgtagtgacc cccaaaagtt agactttttt ggagtggaga aatccactcc
    38461 ttttttttat gctctaaaat gatattttta cacaaccgtt acggttgttt tacgaaattg
    38521 gacg
//
LOCUS       DAYYCQ010000017        38297 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000017 DAYYCQ010000000
VERSION     DAYYCQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.017"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACHAYF_000923"
     CDS             complement(451..1761)
                     /locus_tag="ACHAYF_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8578380.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACHAYF_000924"
     CDS             complement(1773..3191)
                     /locus_tag="ACHAYF_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8578381.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACHAYF_000925"
     CDS             complement(3431..4999)
                     /locus_tag="ACHAYF_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8578382.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACHAYF_000926"
     CDS             complement(5003..5413)
                     /locus_tag="ACHAYF_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8578383.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACHAYF_000927"
     CDS             complement(5410..6333)
                     /locus_tag="ACHAYF_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8578384.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACHAYF_000928"
     CDS             complement(6330..7208)
                     /locus_tag="ACHAYF_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8578385.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACHAYF_000929"
     CDS             complement(7195..8532)
                     /locus_tag="ACHAYF_000929"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8578386.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACHAYF_000930"
     CDS             complement(8535..9638)
                     /locus_tag="ACHAYF_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8578387.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACHAYF_000931"
     CDS             complement(9712..11220)
                     /locus_tag="ACHAYF_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8578388.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACHAYF_000932"
     CDS             complement(11186..11878)
                     /locus_tag="ACHAYF_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8578389.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACHAYF_000933"
     CDS             complement(11900..12874)
                     /locus_tag="ACHAYF_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578390.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ACHAYF_000934"
     CDS             13174..14046
                     /locus_tag="ACHAYF_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578391.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="ACHAYF_000935"
     CDS             complement(14504..16702)
                     /locus_tag="ACHAYF_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578392.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="ACHAYF_000936"
     CDS             16934..17926
                     /locus_tag="ACHAYF_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8578393.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="ACHAYF_000937"
     CDS             17986..19407
                     /locus_tag="ACHAYF_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8578394.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="ACHAYF_000938"
     CDS             19400..20899
                     /locus_tag="ACHAYF_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8578395.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="ACHAYF_000939"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="ACHAYF_000939"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8578396.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="ACHAYF_000940"
     CDS             22491..23174
                     /locus_tag="ACHAYF_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8578397.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="ACHAYF_000941"
     CDS             23176..24012
                     /locus_tag="ACHAYF_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8578398.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="ACHAYF_000942"
     CDS             24023..25777
                     /locus_tag="ACHAYF_000942"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578399.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="ACHAYF_000943"
     CDS             complement(26053..26394)
                     /locus_tag="ACHAYF_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8578400.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="ACHAYF_000944"
     CDS             complement(26462..28633)
                     /locus_tag="ACHAYF_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8578401.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="ACHAYF_000945"
     CDS             28844..29701
                     /locus_tag="ACHAYF_000945"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8578402.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="ACHAYF_000946"
     CDS             complement(29772..30629)
                     /locus_tag="ACHAYF_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8578403.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="ACHAYF_000947"
     CDS             complement(30614..33316)
                     /locus_tag="ACHAYF_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8578404.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="ACHAYF_000948"
     CDS             complement(33329..34618)
                     /locus_tag="ACHAYF_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8578405.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="ACHAYF_000949"
     CDS             34768..35814
                     /locus_tag="ACHAYF_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8578406.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="ACHAYF_000950"
     CDS             35816..37969
                     /locus_tag="ACHAYF_000950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8578407.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11302 a         7868 c         6810 g        12317 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcttccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcagggc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagcatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taaaccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg actcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga cagagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccatt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaattt
//
LOCUS       DAYYCQ010000018        36091 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000018 DAYYCQ010000000
VERSION     DAYYCQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.018"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACHAYF_000951"
     CDS             complement(27..1259)
                     /locus_tag="ACHAYF_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8578408.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACHAYF_000952"
     CDS             1514..2083
                     /locus_tag="ACHAYF_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8578409.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACHAYF_000953"
     CDS             complement(2261..2701)
                     /locus_tag="ACHAYF_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8578410.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACHAYF_000954"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACHAYF_000954"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8578411.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="ACHAYF_000955"
     CDS             3655..4578
                     /locus_tag="ACHAYF_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8578412.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="ACHAYF_000956"
     CDS             complement(4803..5042)
                     /locus_tag="ACHAYF_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578413.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="ACHAYF_000957"
     CDS             complement(5047..5466)
                     /locus_tag="ACHAYF_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8578414.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACHAYF_000958"
                     /gene_synonym="mefH"
     CDS             complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACHAYF_000958"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8578415.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..7673)
                     /locus_tag="ACHAYF_000959"
     CDS             complement(7038..7673)
                     /locus_tag="ACHAYF_000959"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8578416.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7787..7879)
                     /locus_tag="ACHAYF_000960"
                     /pseudo
     CDS             complement(7787..7879)
                     /locus_tag="ACHAYF_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8015..8650)
                     /locus_tag="ACHAYF_000961"
     CDS             complement(8015..8650)
                     /locus_tag="ACHAYF_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8578417.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9162..10304
                     /locus_tag="ACHAYF_000962"
     CDS             9162..10304
                     /locus_tag="ACHAYF_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8578418.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10297..10503
                     /locus_tag="ACHAYF_000963"
     CDS             10297..10503
                     /locus_tag="ACHAYF_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8578419.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10582..11775
                     /locus_tag="ACHAYF_000964"
     CDS             10582..11775
                     /locus_tag="ACHAYF_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8578420.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11980..13119
                     /locus_tag="ACHAYF_000965"
     CDS             11980..13119
                     /locus_tag="ACHAYF_000965"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8578421.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13112..13912
                     /locus_tag="ACHAYF_000966"
     CDS             13112..13912
                     /locus_tag="ACHAYF_000966"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8578422.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13912..14739
                     /locus_tag="ACHAYF_000967"
     CDS             13912..14739
                     /locus_tag="ACHAYF_000967"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8578423.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14744..15451
                     /locus_tag="ACHAYF_000968"
     CDS             14744..15451
                     /locus_tag="ACHAYF_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8578424.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15551..16417
                     /gene="rfbA"
                     /locus_tag="ACHAYF_000969"
     CDS             15551..16417
                     /gene="rfbA"
                     /locus_tag="ACHAYF_000969"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8578425.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16431..17003
                     /gene="rfbC"
                     /locus_tag="ACHAYF_000970"
     CDS             16431..17003
                     /gene="rfbC"
                     /locus_tag="ACHAYF_000970"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8578426.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17025..18053
                     /gene="rfbB"
                     /locus_tag="ACHAYF_000971"
     CDS             17025..18053
                     /gene="rfbB"
                     /locus_tag="ACHAYF_000971"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8578427.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18151..19002
                     /gene="rfbD"
                     /locus_tag="ACHAYF_000972"
     CDS             18151..19002
                     /gene="rfbD"
                     /locus_tag="ACHAYF_000972"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8578428.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19036..21069
                     /locus_tag="ACHAYF_000973"
     CDS             19036..21069
                     /locus_tag="ACHAYF_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578429.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21113..22393
                     /locus_tag="ACHAYF_000974"
     CDS             21113..22393
                     /locus_tag="ACHAYF_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8578430.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22603..23409
                     /locus_tag="ACHAYF_000975"
     CDS             22603..23409
                     /locus_tag="ACHAYF_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578431.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23421..24641
                     /locus_tag="ACHAYF_000976"
     CDS             23421..24641
                     /locus_tag="ACHAYF_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578432.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24631..26217
                     /locus_tag="ACHAYF_000977"
     CDS             24631..26217
                     /locus_tag="ACHAYF_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8578433.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26256..28394
                     /locus_tag="ACHAYF_000978"
     CDS             26256..28394
                     /locus_tag="ACHAYF_000978"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8578434.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28762..29745)
                     /locus_tag="ACHAYF_000979"
     CDS             complement(28762..29745)
                     /locus_tag="ACHAYF_000979"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8578435.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30123..31514
                     /locus_tag="ACHAYF_000980"
     CDS             30123..31514
                     /locus_tag="ACHAYF_000980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8578436.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31530..32570
                     /locus_tag="ACHAYF_000981"
     CDS             31530..32570
                     /locus_tag="ACHAYF_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8578437.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32589..33674
                     /locus_tag="ACHAYF_000982"
     CDS             32589..33674
                     /locus_tag="ACHAYF_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8578438.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33707..34669
                     /locus_tag="ACHAYF_000983"
     CDS             33707..34669
                     /locus_tag="ACHAYF_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8578439.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            34662..36089
                     /locus_tag="ACHAYF_000984"
     CDS             34662..36089
                     /locus_tag="ACHAYF_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8578440.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAMDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT        11748 a         6067 c         7037 g        11239 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaac gctttggtta
     7381 gaattgaata ccgctgtctt aaatatttct cttaactttt ctaaaccgtt taaactatcg
     7441 ttatctctaa ttttagcaaa tattactgca ttttcatttc caattctttg aaaaattgct
     7501 tttaaaatat cttcctttga atcgaaatga tgataaatag cacctttaga aagttttgtt
     7561 tcattgataa tatcttgcaa tgatgtgtta tcatatcctt tttcacaaaa catttttgta
     7621 gaaacatcta aaatgagttt tactgtttct tctggatatt tatttcgtgc cataacttac
     7681 ctccaataca aaccgttggt ctgattaaag tatatcttag tattttatgc ttgtcaatat
     7741 aaagacttgc aattttataa aaatgcagaa tgcaagcctt tttcatttat tctacaatct
     7801 tccagttgct ttccttatgc ggtgtcaatt catactgtga tacctgcgtt gcctttgcct
     7861 gatttaccac aaatcagatt ttatcccttt cttcctcttg attgcccaca acagaaaaga
     7921 aaaagccctg tcaagagctt tgccaccatt ggtggtgctt tgcactcttg atcgggcttt
     7981 ttgtttgctg tatcttctcg caagaggaaa aaagttactc gtcctcatca tcaaaattgt
     8041 tattccttaa tacttctcgt aacagttcca tatcttcttt tgagttcggg tttatcattt
     8101 tcacgacttg ataaggtgtc ttatacttat gtccctctat gctttcccca aacatatcca
     8161 tactgtataa atcatcatag cggtctaata atttttgaaa cttcaaatgc tggataaatt
     8221 ctttgattgg gtaaaggtcg gacgcttcaa tgcttttccc gttgatatag tctgtaatat
     8281 attcccgtaa cttttctaac tcatattcca gccattcttc ctcgctgtca aagaagttat
     8341 catagtgtcc atgtttcagt tctgcattta gacgttcttc ggctcttaaa aactgacaga
     8401 cttccttttc agattgattg acatacttcc attctcccga caacaattca ttgggcgtaa
     8461 cgtccagcac ttccattatc ttttccagcg tttcataggc aggatagttc aaccctcttt
     8521 caatcttgga aaggctctgc atattgatac cgattttgtc cgcaagttcc tgttgtttca
     8581 ttcccctaaa ttttcttatg gtctgtatat tatttcctaa atggcttatt ttcattgcat
     8641 aaccctccat atctcgttat atcttcgcta ttataagtct atcatgctta atatgatttg
     8701 taaaggcaaa aaaagatatt gacaaataag catataagaa ttactatgtt cttgtaatcg
     8761 aataagcaca atagaatgaa atataaaaac attcaaaaag aaatacctat ctgattaaat
     8821 ccgtagatat gaatatcggt tgaaatggaa cttgttagaa aaggcaaaac agaacttgct
     8881 gacaaagtat ttctgacacg gtggcggaag cctctcaggc ttaccgccat gacaccttgt
     8941 gtcaagggga agaaatgctt tgtcggctgg cgagcctgcg agccttgtct gtgcccccgt
     9001 tatctaacag gggggcacta tttcacaaaa gacaagtcaa aaaccataca cccccaaacc
     9061 tgtgagtagt aatgagaggt tttaagaaga tagtacatat cacacttttt gaaaggtggt
     9121 ttacaggtat ttcaaggcta ttgtgggatt ggaggaatac aatgaacaat ttagaatttg
     9181 cacttgaaat gaagaacaaa cgtcttgcca gcggtctttc acaaggagaa cttgcaagtc
     9241 ttacccatgt atcaagatat agtatcaacc gctttgagaa cggaaaagct aacgccagca
     9301 aggaaacaca gaacattatc ctgcgttgtc tgaattacta tatctgcgat aagccttttt
     9361 atctgctcgt tgattatctg tctgtccgtt ttccaacgac tgacgcattg gaagttatcc
     9421 gaaaggtgct tggcatgaaa gcagattatt ttatccatta tgactatggg tattatggct
     9481 ataaggagca ttatgcttac ggggaaatca aggtcatggc ttctgatgat gaacacatgg
     9541 gcgtattttt agaattaaaa ggggcaggct cacgcaacat ggaatatgtc ttacaggcac
     9601 agaacaggga ctggtattca ttcttaaacc gctgtcttga ctgtggcggt gttatccgac
     9661 gttttgactt ggcaatcaat gatatgtgcg gtctgctgga tattcctgtg ctatctgaaa
     9721 aatacagaaa tggtggtgcg gaatgtcgct gtaaaaacta tgaaaatgta caaggtggaa
     9781 aattaagcgg aaaaaaccgt aatttagcaa gtacccttta tatcggctca aaggcaagca
     9841 caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aacgaaaaag aaacataccg
     9901 atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcggtacag gcagttgaag
     9961 aactgttatt gacgtataac cctcatgggc tggtgtttta tctcattacc gattttgtgg
    10021 aatttcctga ctatccattg tgggagatat tcatttccca tgacagttta ccttttgaaa
    10081 tgaaccctgt acctgtcaat atggaacgca ctctgcaatg gctggaaaga caggtcatgc
    10141 cgtcaatcgt gatgatagag gaaatcgaca ggctgacagg ttcaaactac atgaaaatga
    10201 ttgatgaatg tacccacctt tctgaaaaac aggaaatgct tgtggaacag atgtgtacgg
    10261 acatatcaga cgtaatcgaa agtgaggggg tgttttatga gtaatacaca ggatattccc
    10321 gtatgggaaa aatacaccct taccattgaa gaagcgtcaa agtatttccg cattggagaa
    10381 aacaagttaa gacgattggc agaggaaaac aaggacgctg gctggctcat tatgaatggc
    10441 aaccgcatac agattaaacg ccgacagttt gaaaaggtta ttgataaatt ggacgcaatc
    10501 taatgcaaat gagccttgta tgtgttatga tgaacacaag tcatatcaag gctctttcca
    10561 acaaggaaag gagcagacac catgaaagaa aaaagacggg ataacaaagg tcgtatcctg
    10621 catactggag agagccaacg aacagacgga aaatacttat ataaatatgt agacgcattg
    10681 ggaaacacaa aatatgtgta tgcttggaga ttgacaccca cagacccgac accaaaggga
    10741 aaacgggaaa aaccctcact tcgagaactg gaacagcaga taagacggga tattgaggac
    10801 ggtatcgaca gcacaggcaa gaaaatgacg ctttgccaac tctatgccaa acagaacgca
    10861 cagaggacaa atgtgaagaa aagcacacag aaacaacggg aacaactcat gcggttatta
    10921 aaagaggaca agttaggtgc taggagcatt gatacgataa aaccctctga cgctaaagaa
    10981 tgggcgttac gcatgaaaga caaaggcttt tcctacaata ccattaacaa ccataaacgc
    11041 tcgttaaaag cgtcattcta catagccata caagatgatt atgtgaggaa aaaccctttt
    11101 gatttcaagc taagtgaagt catagaaaat gataccaaag agaaagtcgc attgacagag
    11161 gaacaggagc aagccttatt gtcatttatt aagacggaca acgtgtatca caagcattat
    11221 gatgatgtgc tgatactgtt aaagacagga cttcgtatct cggaactgtg cggactgaca
    11281 gtagccgaca ttgatttcaa gaatgaagtt gtgattatcg accaccagtt actaaagagt
    11341 aaggaacagg gctattatat tgaaacgcct aagacgaaaa gcgggataag gcaagtgcca
    11401 ttaagcaaag aaacgataca ggcatttcaa cgggttatca acagccgacc aaagataaag
    11461 tcgatagagg tagacggaca cagcggtttt gtgtttatca atccgaaagg taatccaaaa
    11521 gttgccattg attataacgc cttatttatc cgtatggtaa agaaatacaa caagcaccac
    11581 aaggacaatc ccttgccaca tattacaccg catacgctac gccatacatt ctgcacaagg
    11641 ctggcaagca agaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc
    11701 agtatcacaa tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag
    11761 cgtctaatcg catagaagta tttaccacga ttttaaccac gattgacagg gaaaatataa
    11821 gaagatagac ctagatatgc gaggtttacc acaaaagcaa aatgcccgta gagccgataa
    11881 aataaggctt tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat
    11941 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    12001 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    12061 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    12121 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    12181 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    12241 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    12301 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    12361 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    12421 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    12481 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    12541 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    12601 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    12661 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    12721 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    12781 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    12841 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    12901 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    12961 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    13021 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    13081 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    13141 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    13201 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    13261 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    13321 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    13381 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    13441 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    13501 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    13561 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    13621 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    13681 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    13741 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    13801 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    13861 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    13921 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    13981 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    14041 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    14101 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    14161 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    14221 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    14281 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    14341 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    14401 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    14461 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    14521 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    14581 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    14641 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    14701 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    14761 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    14821 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    14881 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    14941 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    15001 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    15061 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    15121 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    15181 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    15241 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    15301 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    15361 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    15421 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    15481 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    15541 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    15601 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    15661 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    15721 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    15781 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    15841 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    15901 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    15961 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    16021 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    16081 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    16141 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    16201 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    16261 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    16321 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    16381 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    16441 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    16501 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    16561 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    16621 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    16681 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    16741 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    16801 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    16861 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    16921 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    16981 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    17041 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    17101 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    17161 ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    17221 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    17281 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    17341 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    17401 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    17461 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    17521 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    17581 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata
    17641 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    17701 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    17761 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    17821 aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact
    17881 tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta
    17941 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    18001 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    18061 aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    18121 taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa
    18181 ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat
    18241 gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa
    18301 cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca
    18361 aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa
    18421 gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    18481 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    18541 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    18601 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    18661 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    18721 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    18781 aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta
    18841 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    18901 gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca
    18961 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag
    19021 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    19081 gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    19141 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    19201 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    19261 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    19321 gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    19381 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    19441 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    19501 gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta
    19561 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    19621 ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat
    19681 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    19741 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    19801 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    19861 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    19921 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    19981 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    20041 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    20101 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    20161 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    20221 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    20281 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    20341 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    20401 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    20461 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    20521 tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    20581 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    20641 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    20701 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    20761 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    20821 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    20881 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    20941 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    21001 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    21061 gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat
    21121 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    21181 actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat
    21241 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    21301 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    21361 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    21421 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    21481 ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    21541 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    21601 caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    21661 cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt
    21721 tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    21781 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac
    21841 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    21901 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    21961 tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg
    22021 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    22081 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    22141 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    22201 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    22261 atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat
    22321 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    22381 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    22441 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    22501 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    22561 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    22621 ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac
    22681 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    22741 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    22801 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    22861 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    22921 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta
    22981 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    23041 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    23101 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    23161 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    23221 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    23281 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    23341 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    23401 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    23461 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    23521 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    23581 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    23641 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    23701 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    23761 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    23821 ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg
    23881 ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag
    23941 cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    24001 agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt
    24061 gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag
    24121 taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata
    24181 aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt
    24241 tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    24301 taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa
    24361 cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc aagattcctt
    24421 ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    24481 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga
    24541 caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    24601 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    24661 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    24721 tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg
    24781 agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga
    24841 ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat
    24901 tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa
    24961 gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat
    25021 aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta
    25081 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg
    25141 ggattattca ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    25201 tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt
    25261 gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca
    25321 gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat
    25381 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    25441 cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc
    25501 gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc
    25561 aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg
    25621 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
    25681 ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt
    25741 tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga
    25801 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
    25861 tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa
    25921 aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa
    25981 atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct
    26041 gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa
    26101 cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt
    26161 tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata
    26221 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    26281 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    26341 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    26401 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    26461 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc
    26521 aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg
    26581 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    26641 caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat
    26701 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga
    26761 aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg
    26821 ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt
    26881 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat
    26941 cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca
    27001 gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata
    27061 atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc
    27121 cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg
    27181 atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc
    27241 acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg
    27301 aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata
    27361 tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta
    27421 atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt
    27481 ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg
    27541 cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg
    27601 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa
    27661 tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca
    27721 agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc
    27781 gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    27841 atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc
    27901 gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag
    27961 tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt
    28021 gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    28081 tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag
    28141 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    28201 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag
    28261 aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata
    28321 ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt
    28381 tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg
    28441 ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag
    28501 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca
    28561 aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg
    28621 tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca
    28681 gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc
    28741 gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa
    28801 agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt
    28861 cttagcgtca atataaagaa catttccact attaccaatt gctgaatata tttcgttctc
    28921 agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact
    28981 ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata
    29041 catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa
    29101 accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga
    29161 cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt
    29221 tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact
    29281 tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc
    29341 ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga
    29401 acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg
    29461 cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata
    29521 atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag
    29581 caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc
    29641 attccattca tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt
    29701 tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac
    29761 tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta
    29821 atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata
    29881 aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc
    29941 aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag
    30001 aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc ttataaacta
    30061 ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat
    30121 atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg
    30181 ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc
    30241 cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg
    30301 cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt
    30361 ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg
    30421 gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt
    30481 tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa
    30541 aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa
    30601 agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca
    30661 aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa
    30721 aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat
    30781 ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg
    30841 aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca
    30901 tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca gcaattttag
    30961 tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa
    31021 aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac
    31081 cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg
    31141 tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag
    31201 gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact
    31261 acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg
    31321 tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga
    31381 tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc
    31441 aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa
    31501 aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac
    31561 cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt
    31621 atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca
    31681 ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc
    31741 ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg
    31801 atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga
    31861 gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa
    31921 tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta
    31981 taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct
    32041 atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg
    32101 atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag
    32161 tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca
    32221 agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata
    32281 aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata
    32341 gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc
    32401 cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa
    32461 agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg
    32521 ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg
    32581 aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt
    32641 ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga
    32701 tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc
    32761 gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata
    32821 agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa
    32881 ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa
    32941 ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat
    33001 tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt
    33061 ttcaaaacca cccctttgag atagttcata atggtattga tttagagctt tatagtaaaa
    33121 attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg
    33181 tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt
    33241 tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta
    33301 gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag
    33361 gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc
    33421 tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct
    33481 tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag
    33541 atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa
    33601 cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag agtatatata
    33661 gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga aaatatgtta
    33721 tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc gattgaacga
    33781 aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat ttattgaagc
    33841 atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta aaatttattg
    33901 gggtggtttt tccattgtac aagcagaaat caatttagta aagagagcat tacaaaatga
    33961 gaaatattta aaatatgtat tattatctgg tgctgattat ccaatcaaag ataatgagta
    34021 tatttataat tattttaaaa agaatagttc agtggagttt attcgtggaa tagatctaga
    34081 tcaaattaaa cataaagaat tttattataa acatatagat gtatatcaaa aacatgatta
    34141 tcctagaatt aataggaata acacaactgc atttaaaatt tttagagcta taattaatcg
    34201 ttgtctgagg atgattaaac ttcccccaaa aattagacac cataaatttg atttgtatca
    34261 tggttctcaa tggtgggctt tgtcgaaaga atgcctaaca gaattaatac aaatgtatga
    34321 acaaaatcaa caagactacc tgaattttaa aataggtatg tttgccccag atgaaaaatt
    34381 ttttcataca ctttttttta attcctcgtt caaaaataaa aatgttattg gaggaccaga
    34441 tttaccactt gaattgaaaa atattgaaga aactacttta caaacttcga aattagcaaa
    34501 tattcatatt attgatcctt ccatgaacaa atggtttgat gaaagggata tagatcaaat
    34561 aaaagaaagt gaaaaattat ttgtaagaaa agttaaatcg gattactctg ataaactatt
    34621 aaatagaatt gataaagaaa ttttgaaagt tggaaatata gatgaataga atagtacctg
    34681 ttttagagaa gagtttatta agtcgagatg cgtttcgagt aataaattta atcatttgtc
    34741 tgctttattt tatgcctagt acttttaatg taactaatat cccgatgaaa atagcttttg
    34801 cttggggtgg aattatagtt ttgtatgagg tttttataaa gaaaaatttt tttaaaatgc
    34861 gtttttcttg gctactgatt ggagcaatga ttagttttat cttaactata atcattaaca
    34921 gagaaaattt tttaattcca tctatttata attttggata tttgctgatt actttgatag
    34981 taatttttcc tgcagacttt tcacaaagtg aagaggaaaa aaagaaaaaa atggtgtatt
    35041 ttaataatgt tttcattatc ataatttttt tagctgcgct aatatctatc tatcaattta
    35101 tttttttgat ttcttatcat gtcccaacag gaacgccagg attacttgca agacaaggtt
    35161 ttattgaaca tcggttattt ggtgtctata caagtccaaa tgttggagct atgtttggat
    35221 atacttctat tattttaagt ttaattagtg tattaatttc aaaaataaca aaaacgattc
    35281 gtgtttttta tatgatgaat tttatcgtac aaatatttta tattacactt tcttcgtcta
    35341 gaggtgccca ggtagtaatt tgttcttttt tagtattatt tcttttgttg tttgccaatt
    35401 caaaatttag aaagagcatt agagaagtac tacctgtaaa aatcaaatgg atggtaagca
    35461 tattattagt tctattggta tatttttctt ttggtactaa ttatgctatg gattttttag
    35521 caacaattcc tgtatcgatt gatagaaaag tagaagttat agagtcaaat gaatcagata
    35581 aaactccact tccagaaaaa gaagtaatat tacagcattc tagtgataac tccgaaattt
    35641 cagctggtcg ttttacgatt tggaaagctg cgcttaaagt tataagacca actttgttat
    35701 ttggttatgg agagaccgat ttctatggga atagcgatgc aaaatttttg aaaaaagtag
    35761 atcttgatac tacagatgtg aatgaactaa aaagagcaca tggaaacatg cataatggat
    35821 ttcttcaagt acttgtatca agtggtataa ttgcttttat atgtatctat gctttctatg
    35881 tgttaaatat ctttagttta ctcaaaacat tttttagaag taaagtgaat tatgcgtttc
    35941 ttggtattgt tttaattttt atactatcta tttttataga tgaaatggtg gaggctcatg
    36001 tacttttcaa taagcgcgat gtcatttcaa ttgttttctg gtattatttg ggaagcattt
    36061 cttacttgta tttaaaaaag gagaattaat a
//
LOCUS       DAYYCQ010000019        34955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000019 DAYYCQ010000000
VERSION     DAYYCQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.019"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHAYF_000985"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHAYF_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8578441.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHAYF_000986"
     CDS             complement(2020..2922)
                     /locus_tag="ACHAYF_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8578442.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHAYF_000987"
     CDS             complement(2919..3611)
                     /locus_tag="ACHAYF_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8578443.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHAYF_000988"
     CDS             complement(3654..4295)
                     /locus_tag="ACHAYF_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8578444.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHAYF_000989"
     CDS             complement(4333..4647)
                     /locus_tag="ACHAYF_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578445.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHAYF_000990"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHAYF_000990"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8578446.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHAYF_000991"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHAYF_000991"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8578447.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHAYF_000992"
     CDS             complement(5984..6916)
                     /locus_tag="ACHAYF_000992"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8578448.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHAYF_000993"
     CDS             complement(6913..7689)
                     /locus_tag="ACHAYF_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8578449.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHAYF_000994"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHAYF_000994"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8578450.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHAYF_000995"
     CDS             complement(10933..12009)
                     /locus_tag="ACHAYF_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8578451.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHAYF_000996"
     CDS             complement(12014..13294)
                     /locus_tag="ACHAYF_000996"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8578452.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHAYF_000997"
     CDS             complement(13313..14239)
                     /locus_tag="ACHAYF_000997"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8578453.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHAYF_000998"
     CDS             complement(14252..15535)
                     /locus_tag="ACHAYF_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8578454.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHAYF_000999"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHAYF_000999"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8578455.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHAYF_001000"
     CDS             complement(16323..17234)
                     /locus_tag="ACHAYF_001000"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8578456.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHAYF_001001"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHAYF_001001"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8578457.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHAYF_001002"
     CDS             complement(17700..18398)
                     /locus_tag="ACHAYF_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8578458.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHAYF_001003"
     CDS             complement(18385..18771)
                     /locus_tag="ACHAYF_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578459.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHAYF_001004"
     CDS             complement(18918..20588)
                     /locus_tag="ACHAYF_001004"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8578460.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHAYF_001005"
     CDS             20771..21004
                     /locus_tag="ACHAYF_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8578461.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHAYF_001006"
     CDS             complement(21103..21534)
                     /locus_tag="ACHAYF_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8578462.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHAYF_001007"
     CDS             21613..22026
                     /locus_tag="ACHAYF_001007"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8578463.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHAYF_001008"
     CDS             complement(22064..23212)
                     /locus_tag="ACHAYF_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8578464.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHAYF_001009"
     CDS             complement(23334..23930)
                     /locus_tag="ACHAYF_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8578465.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHAYF_001010"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHAYF_001010"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8578466.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHAYF_001011"
     CDS             27160..27591
                     /locus_tag="ACHAYF_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8578467.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHAYF_001012"
     CDS             complement(27636..28985)
                     /locus_tag="ACHAYF_001012"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8578468.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHAYF_001013"
     CDS             complement(29055..30218)
                     /locus_tag="ACHAYF_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8578469.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHAYF_001014"
     CDS             30347..30670
                     /locus_tag="ACHAYF_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578470.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHAYF_001015"
     CDS             complement(30853..32202)
                     /locus_tag="ACHAYF_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8578471.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHAYF_001016"
     CDS             complement(32271..33224)
                     /locus_tag="ACHAYF_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8578472.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHAYF_001017"
     CDS             complement(33326..34516)
                     /locus_tag="ACHAYF_001017"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8578473.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34955)
                     /locus_tag="ACHAYF_001018"
     CDS             complement(34716..>34955)
                     /locus_tag="ACHAYF_001018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578474.1"
                     /translation="KNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFL
                     LVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         9949 a         7639 c         6119 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttctt
//
LOCUS       DAYYCQ010000020        34661 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000020 DAYYCQ010000000
VERSION     DAYYCQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.020"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(313..2205)
                     /locus_tag="ACHAYF_001019"
     CDS             complement(313..2205)
                     /locus_tag="ACHAYF_001019"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578475.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="ACHAYF_001020"
     CDS             complement(2202..3929)
                     /locus_tag="ACHAYF_001020"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578476.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="ACHAYF_001021"
     CDS             complement(3945..4595)
                     /locus_tag="ACHAYF_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8578477.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="ACHAYF_001022"
     CDS             4799..5095
                     /locus_tag="ACHAYF_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8578478.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="ACHAYF_001023"
     CDS             5287..5907
                     /locus_tag="ACHAYF_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8578479.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="ACHAYF_001024"
     CDS             complement(5999..7165)
                     /locus_tag="ACHAYF_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8578480.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="ACHAYF_001025"
     CDS             complement(7177..7530)
                     /locus_tag="ACHAYF_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8578481.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="ACHAYF_001026"
     CDS             7658..8680
                     /locus_tag="ACHAYF_001026"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8578482.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8699..8860
                     /locus_tag="ACHAYF_001027"
     CDS             8699..8860
                     /locus_tag="ACHAYF_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578483.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9119..9553)
                     /locus_tag="ACHAYF_001028"
     CDS             complement(9119..9553)
                     /locus_tag="ACHAYF_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578484.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="ACHAYF_001029"
     CDS             complement(9663..11522)
                     /locus_tag="ACHAYF_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8578485.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11523..11663
                     /locus_tag="ACHAYF_001030"
     CDS             11523..11663
                     /locus_tag="ACHAYF_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8578486.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11640..12800)
                     /locus_tag="ACHAYF_001031"
     CDS             complement(11640..12800)
                     /locus_tag="ACHAYF_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8578487.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13107..14531)
                     /gene="araA"
                     /locus_tag="ACHAYF_001032"
     CDS             complement(13107..14531)
                     /gene="araA"
                     /locus_tag="ACHAYF_001032"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8578488.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14547..15245)
                     /locus_tag="ACHAYF_001033"
     CDS             complement(14547..15245)
                     /locus_tag="ACHAYF_001033"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8578489.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15202..16863)
                     /locus_tag="ACHAYF_001034"
     CDS             complement(15202..16863)
                     /locus_tag="ACHAYF_001034"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8578490.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17081..18481
                     /locus_tag="ACHAYF_001035"
     CDS             17081..18481
                     /locus_tag="ACHAYF_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8578491.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18733..19812)
                     /locus_tag="ACHAYF_001036"
     CDS             complement(18733..19812)
                     /locus_tag="ACHAYF_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8578492.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19898..20857)
                     /locus_tag="ACHAYF_001037"
     CDS             complement(19898..20857)
                     /locus_tag="ACHAYF_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8578493.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20869..22380)
                     /locus_tag="ACHAYF_001038"
     CDS             complement(20869..22380)
                     /locus_tag="ACHAYF_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8578494.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22402..22965)
                     /locus_tag="ACHAYF_001039"
     CDS             complement(22402..22965)
                     /locus_tag="ACHAYF_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8578495.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22971..23834)
                     /locus_tag="ACHAYF_001040"
     CDS             complement(22971..23834)
                     /locus_tag="ACHAYF_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578496.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23848..24744)
                     /locus_tag="ACHAYF_001041"
     CDS             complement(23848..24744)
                     /locus_tag="ACHAYF_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578497.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24919..26208)
                     /locus_tag="ACHAYF_001042"
     CDS             complement(24919..26208)
                     /locus_tag="ACHAYF_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8578498.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26180..27697)
                     /locus_tag="ACHAYF_001043"
     CDS             complement(26180..27697)
                     /locus_tag="ACHAYF_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8578499.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27895..28785
                     /locus_tag="ACHAYF_001044"
     CDS             27895..28785
                     /locus_tag="ACHAYF_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8578500.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28858..29682)
                     /gene="nadE"
                     /locus_tag="ACHAYF_001045"
     CDS             complement(28858..29682)
                     /gene="nadE"
                     /locus_tag="ACHAYF_001045"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8578501.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29694..31166)
                     /locus_tag="ACHAYF_001046"
     CDS             complement(29694..31166)
                     /locus_tag="ACHAYF_001046"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8578502.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31803..32165)
                     /locus_tag="ACHAYF_001047"
     CDS             complement(31803..32165)
                     /locus_tag="ACHAYF_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8578503.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32388..33140
                     /locus_tag="ACHAYF_001048"
     CDS             32388..33140
                     /locus_tag="ACHAYF_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8578504.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33280..34203)
                     /locus_tag="ACHAYF_001049"
     CDS             complement(33280..34203)
                     /locus_tag="ACHAYF_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8578505.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34218..34490)
                     /locus_tag="ACHAYF_001050"
     CDS             complement(34218..34490)
                     /locus_tag="ACHAYF_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578506.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10230 a         7212 c         5979 g        11240 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc ctagatgagg caacataagt atacggtcat tttctaagaa ctcttcttct
    12061 ggaaaatcag tcaagtattt agccagatgt tgtttttcca atgcttgcat aacagcatat
    12121 ttatcaacaa tttcacttct tcctacattg ataagtacag catctttttt cattttttct
    12181 atattcgcct gatcgatcaa tccttttgta tcttctgtca acggcagcat gactacgatg
    12241 taatccgagg tagaaagaac tgtttctaaa tctgcttgcc gaacataatc caactgggca
    12301 tcgcggatag aataacccaa gacatccatc cctaagctat agcaacttag cgctactttt
    12361 gtgcctatcg ctccaagacc aagaagaccg atggttttgc cttgcaattc tcgtcccaca
    12421 taagcacttc ttttgttttc tgcctgttct aaaatattag gacctgttag tgtttgtacc
    12481 atacgacttg cttcgattat cggacgggaa gataagagca aacagcacaa aacaagctct
    12541 tttactgcat tagcatttac acctggtgta ttcatgacga tcgtcccgtt ttcagaagct
    12601 ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg aaattgctag caaatctggt
    12661 gtaagccatt catctttcac tttcgtacta cgaataacta ttccttcggc tggcaaatca
    12721 tctattattt tgaaatattc cccttgtaac ttttctatct cttgttctgg aatagcatca
    12781 atcaaatgaa cgtgatacat aaaaccccct ccttactaac aagtatctcg aaatttctgc
    12841 aattagacaa ctgtttacca tttgacttcc tagaagcaaa aagagcagtt agagcagagt
    12901 aggctgagtg ataaaaataa ggctatgaca agttactgcc ataagcctta ttttcgagag
    12961 caaaatattg agggtgagat atcttgataa atggtgttcc gttagctgac attgataata
    13021 agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa tctcttctta tttcttaaaa
    13081 ctaagtggct ttttcacaac ctctttttat ctaataacca ctgtttccaa ctcaactaat
    13141 tttgcccacg tttcaatttg atctgttgtt agatttagcg agacaacggt atgatgaccg
    13201 ccacctgcct gaatccatga atggacacct tcatggaaat tcggtttagt tttccataac
    13261 acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt caaaggcttc tacttcattg
    13321 atcagcagtt tataatgcgt accgaaatct gccatcgata cgactacccc ttctcctgct
    13381 ttcccatcaa atacaagacg cgccggatct tctctatctc ccattgataa aggagaaaca
    13441 acgattcttg gtttcgtcgc agcaagcgtc ggatctactt ccatcatatg ggattccaag
    13501 attgcttctt ggccactagc tagctcgtaa gtataatctt ccatgaatcc tgtatctttc
    13561 cctcgagcca tgattttcag caaccgatca atcgctgcag ttttccagtc gccttcccca
    13621 gcaaaaccgt atccttctgc catcaaacgt tgtacagcta gtccaggtag ttgtttcatt
    13681 ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc ctccttcttc taagaaacgg
    13741 cgaataccga tttcctgctg agcttgtact tttacatgtt cctcccatgt cttttcttca
    13801 taatcaccat agtcgaaggt atacaattcc ttgtactctt caaataattg atcgacttct
    13861 tcgtctgata cgcgatcgat tacttgtaca agatctccga taccaaaata atcaacagtc
    13921 caaccaaatt gaatctgtgc ttccactttg tctccttctg tgacacccac gtttctcatg
    13981 ttgtcaccaa aacgagccac ttttattcca aaactttcat tataggctac tgcaacatcc
    14041 atccaatcag caatctcttt ttggatctca ggtcgtttcc agtagccaac gacgatttta
    14101 ttttgtttat ttaatcgagc attgatgaac ccatattcgc ggtcaccatg agcggattga
    14161 tttaaattca tgaaatccat atcgatcgtt ttccaaggaa tgctttcgtt atattgcgta
    14221 gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc ggatccacat ttttgctggt
    14281 gagaaagtat gcatccaagt gatcacacct gctacttctt ctcgataatt gacctctttc
    14341 atgatttttg tgatcgtatc agcagtaact gctaattctt gaaggcggat tggataaggc
    14401 aattggccat tttcattcag ttcatcgacc atttcttgtg catgtttctt tacttctcgc
    14461 agtgcttctt ccccatataa atgctgtgat cctacaacaa accaaaattc tttttctcca
    14521 atattcaaca taattagcca ctccttttat ttgttttgtc cgtaatacgc attttttccg
    14581 tgttttcgta aataatgttt atctaaaatc cgttgaggca gctcttctga aaatgaattg
    14641 agctgtctgg taaataaatt catcttgcat acttcatcta gtacgactgc gttcattacc
    14701 gcactttgcg catctttccc ccaagtaaat ggaccgtgtc cgtgtaaaag tacccctgga
    14761 attgctaagg gatcaatttt gcgttctttg aatgtttcta tgataacttt tcctgtttca
    14821 tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc gagcacaagg taccgatcca
    14881 taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat ccaatcctgc ttgtgcccag
    14941 atcgtcgccc atgtcgagtg agtatggacg attcctccga tttgaggaaa tgaacgatat
    15001 aaaacagcat gagtaggcgt atccgaagag ggattgagtt taccttcaat gatatgattg
    15061 tccaagtcca caacgaccat gtcgtctgct gtcatctctt cataactgac tccgctaggt
    15121 ttgataacga aatatcgtgt ttcaggatca aaagcgctca catttcccca tgtatatttg
    15181 acgagtccgt gtttaggcaa gtcaagattt gcttggtata cttcttcttt cagttgttcc
    15241 agcattttct atcactcctt tttcttgcaa ttgatgaatc gctgtttttt caatgtcgat
    15301 cccctcacgg taacgtctca taaatatctg atagcctgct atttcttctg ctgaaggtgt
    15361 gatggtgatt ccttggtctt gtccaaacac gtcttctgcc aagaagtttt ctaaagactt
    15421 cccatgtcga tccatcatat aggctgctaa taacgcgatt ccccacgctc caccttctcc
    15481 ggctgtgtcc atcactgtaa ctggtgcctc catagcagat gctagaatcc gctgtccaac
    15541 ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga tcaatttgga cattctcaga
    15601 tttcaatata tccattccga ttctcattgc accaaaagca ctggctaaat ggatccgcat
    15661 gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca aataatggcc ttccttcatt
    15721 tactcctgta atgttttcac cggaaaaata accataagaa agtaatcccc cgcaatccgt
    15781 atcgccttct agcgctttgt tgaataatac cgtgaataat ttatctctgc ttatctccat
    15841 acccaaagaa ttggtaaatt cttcaaaaat actgacccaa gcattgatat ccgaagagca
    15901 gttattcgta tgaaccatgc ctactagttc tcctgaaggt gtagtgacca tgtcgatttc
    15961 cggatggaca ttttctagtt ctttttctaa tacgatcata gcgaaggcgg aagtaccagc
    16021 cgaaatgttc cctgtccctt tggctacact gttcgtagcg accataccgg ttcctgcatc
    16081 cccttctggc ggacaaaccg ggattcctgg tctcaaattt cccgtagggt cgatcagata
    16141 agcccctgat tcgcttaact tgccagcttc ttctcctgct actagaactt taggcaaaat
    16201 gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac tgaccaaaaa tatttttcat
    16261 ttcttgatgg taggttttcg ttgaaggatc gataggaaac atgcctgatg catctcctac
    16321 gcctagtact ttctcaccag taagctgcca atggacataa cctgcaagcg ttgtcaaaaa
    16381 attaatttct ttcacatgtt cttctttatt caaaatagat tgatacaaat gagcgatact
    16441 ccatctctgc ggaatattgt attgaaattt ttccgttaat attcgttctg cttcttcagt
    16501 gattgtattt cgccaagtac gaaatggtac tagtaactgt tccttgctat caaatgctag
    16561 ataaccgtgc atcattgcac tgaacccaat cgacccgatt gtcgtaagat tctcgccata
    16621 gatttcgaat acttctgctg ccatttttcg gtaactgacg cgcagtcctt tccaaatatc
    16681 ttctaatgaa taggtccaga taccattttc tagtttgttt tcccactcat aactaccagc
    16741 ggcaatagga gtaaaagatg aatcaatcaa tacagctttg atcctagttg atccaaactc
    16801 aatccctaaa gatgtacgac catctatgac atctaacata ctttgcgcat ttacttcctt
    16861 cataatgatt taactccctc cttctcgtca aacctgatca aaccgtcttt ttagtagcgc
    16921 ttacaatgat tattgtatac ttttgtacgt acatgtcaag cagttattta caagaataca
    16981 ataaaaaaca cttgttttgt acgtacataa gttataaaat gaaattgctt acaaaatctg
    17041 atcaaccgcc ttctcgaaat tctagaagga gagaatactt atgaaaaaag aaaaaaaagt
    17101 ttcctcatcg tttatttatt tttttggcgc atttggcggc attctatttg gatatgatat
    17161 tggtgttatg accggcgcct tacctttttt acagcatgat tggggtttgg caggtaaagc
    17221 ttctttgata ggatggatca cgtcttcggt catgttaggt gcgatccttg gcggctcttt
    17281 atctggtctt ctttctgata agcttggacg acgaaaaatg attcttcttt ctgcacttat
    17341 ttttatggct ggttctgtcc tttctgcatc tgcccctcac aatggttctt attttctgat
    17401 tgcagcaagg attcttctag gtttagctgt tggcgctgca tctgccctag ttcctgctta
    17461 tatgtctgaa atggctcctg ctagactaag gggaagattg tcagggatca atcaagtgat
    17521 gattgcttct ggcatgctcc tttcctatgt tgcagattat ttgctaaaag ggttgcctga
    17581 aacgatggct tggcgcgtga tgttagggct cgctgctgtc cccgcgctga ttttattttt
    17641 cggtgtttta gctctaccag agtcaccgcg ttttcttatg caatctggcc gcttggaaga
    17701 ggcaaaaaga gtattgaact atattcggac accaaaagaa gctgaacagg aattcgaaca
    17761 aatccagctg aatgtcaaac aagaaaagac aaccggaact tcttggcata ctttgttttt
    17821 agaaaaatat cgttccttag tcattgccgg tattggtgta gcagtattcc agcaatttca
    17881 aggagctaat gctatttttt actatattcc gctgatcgta gaaaaagcga ctgggcatgc
    17941 agcgagcgat gcattgatgt ggccgatcat ccaaggaatc attttagttg ctggttcttt
    18001 gctctttttg gttattgcag acaagttcaa tcggcgaact ttattgaaaa taggcggtag
    18061 cgtgatggga ctttctttca tcctaccagc tgttttagga actgtactgg atgctcatac
    18121 aaattcgttg ttgattctct tgtttttatg tatttatgtc gcattttatt cttgcacatg
    18181 ggctccactg acatgggtaa tcgtaggcga aatattccca ctagctgtcc gcgggcgagc
    18241 atctggccta gcctcttctt ttaattggat tggttctttc ctagttggct tgctttttcc
    18301 tgtgatgact gcttctatgt ctcaagaaat cgttttcggc atttttggga tcatctgctt
    18361 tttaggggtt ttatttattc aagagatcgt accagaaact cgtgggaaat ctttagagga
    18421 aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt atttctatcc atcattctta
    18481 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaaccgct tagtttccag
    18541 tcacaaggaa caatttcaaa aaacagcttc tcatgtttga tgaatattgt gacttgtgac
    18601 cagttggtag gagaacaccg tttagtggag aggtggtgaa agaaacgttc agccttgagc
    18661 gatgacaaaa gctctcacaa acattgcgac gagtaaccgt aggagcaata agaggcggta
    18721 aaaacccgtc tcttattttt cgttttcttt cactgtttcc cgttcaacta gtacaggttc
    18781 atagagaata tttggctgtg cctgtccttt ttcaatcgtt gtgacgatcc accgtgcggc
    18841 atcttgtccc aacttttctt ttggatgcgt caatgttgtc aattttttat tccctaaatg
    18901 gcttaaagaa gaatcgtctg ttcctacgat cgataggtca tctggaatat gatagccatt
    18961 ttctgtcaac ttatctagca ttaaactagc tatttgatcg ttatagcaaa tgatgccatc
    19021 taaagcttgt tcttctttca accgttgtac tgttttttca gcgattgttt ctcgtgtttc
    19081 cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca ttatactgtt cacacgcgct
    19141 gataaaacct ttcatcctgt attttccttg aagatcgtcg atttttgtga tgaacaacag
    19201 acgctgatgt ccttttttta tcaaatattc tgctgccata aaaccgactt tcacatcatc
    19261 taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca ccatcggaat
    19321 gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat tacttttcgt
    19381 aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac actctttctc
    19441 caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt ctcgtaattc
    19501 attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg tggtgatgac
    19561 accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt catctacata
    19621 tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag cgattgcttg
    19681 ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg agggaatggt
    19741 tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg caatatcttg
    19801 atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat aacgagtctg
    19861 caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac ggaacaggtt
    19921 caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat
    19981 taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga tagatcaaaa
    20041 tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca aattgcttat
    20101 tttttttaga agaaacgaat actggctccg ataatttatg ccatgattct tttaataaaa
    20161 gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct gttgcactac
    20221 cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct tcatttactg
    20281 aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc ttccccttta
    20341 acgtccaagg attatccatt tcggatagat aaagattaga attcccagta atttcaggat
    20401 cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt ccgtctaaac
    20461 tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta ttcattggat
    20521 ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca cgtatagttt
    20581 tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg acttcaggtg
    20641 cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc aggcttgcag
    20701 catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg tagcctggaa
    20761 ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga tctgcccgtt
    20821 gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc acattttctt
    20881 tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt catgtttttc
    20941 tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg tatttttgtc
    21001 caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat tgaaatcatg
    21061 ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag tcagctgatt
    21121 gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt cacaatcata
    21181 aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg ataagtgttg
    21241 catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca gtattaaagg
    21301 tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt gtcgtaaaat
    21361 agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac accaatcgat
    21421 gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta cattccattc
    21481 atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat aatcacaagt
    21541 agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt
    21601 atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact tatcaatata
    21661 gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga tacgatccca
    21721 ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt caattgaatc
    21781 atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt cataagcact
    21841 cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata atttaacgcc
    21901 aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc cttttggaaa
    21961 attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta aattcaccgt
    22021 gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga gtccaaaaga
    22081 atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt ctttcctacc
    22141 tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc gaatgacacc
    22201 taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac catcagctgt
    22261 ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct caacaaatga
    22321 gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt ttactttcac
    22381 ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa tagatacaag
    22441 tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata tgagcagtac
    22501 aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa atactttcca
    22561 tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa tcacatatat
    22621 ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa ttgaaagtac
    22681 gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg ttaacagact
    22741 aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat aaatgatatt
    22801 tcctgatccc tcacctttcc attgcgccat tacataaaaa acagcacatg tagcaggcac
    22861 gatagtaata acaggcaaac acacgattac ccagagaata cctaataaga tgtaatcaga
    22921 aaccacaatc aactgtccat aaagtttagt ctcaactatt ttcatcttcc tcactctttc
    22981 attccagaca tagctgttcc ttgaacaaaa tatttctgtg caaaaatata aaccactagt
    23041 aatggcagca ttgaaataac cgttccagcc attacaggtc cataaaaaga aatgttctgc
    23101 cctgtaaata aagctaaccc tgctggtaaa gttctcattt ttgaactact tgtcaatatc
    23161 aatggataaa gcaagtcatt ccaactattc atcagcgtga aaatccctaa tgataaaaga
    23221 gtaggtttgc tcaatggcaa catgattttc aaaaaaatat taaactcact taatccatca
    23281 atgcgtgctg cctcttccaa agaatctggt aaagtaataa aaaaggaacg catcatgaag
    23341 ataccaaatg cagttgtcat acgaggaatg atcaaacctg cataagtatc aagcatgcca
    23401 agcaaattga cttcaataaa tagaggaatc atgaagactt ggaatgggat catcattgtc
    23461 agcaaaacga aatagaacaa aactgatttt cctttgaaac gcatacgagc aaatgcatac
    23521 cccgcaagag aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa aatcactgta
    23581 tttttgatgt aatctaataa agggatcgtt gtccaaacct taatatagtt atctagggta
    23641 tagctatctg caaatattct aggcggaaat gagataatgt ctttttcgtg tttaaaagaa
    23701 gaaataatca gccacaaaaa aggaaacgca ataaccaaca atataactag catactaatc
    23761 gttcgaatga tttttttacc actaactgct tttcgactat tcacagtaac tgtaatcgag
    23821 ctttctggtg ccatacaggt ttccctccta tttcatttgt ttttctttcc gtgacatgta
    23881 cacattagtg gagatagcta tagttgcaac aatgataaac aaaaccgaag acagtgctga
    23941 tgcatagccc aattcataag gtgctgtaaa tcctcgactg taaatatatt gaacagctgt
    24001 ttctgtctta aattgtgggc caccagcggt tgcaacatat acttggtcaa acacttgcat
    24061 cgaaccaatc agattcgtta aaatacaaaa acctaaggag ggaacgatct gaggtagcgt
    24121 aatgttaaaa aactgctgtc gatttgttgc accatccatt tgagcagatt catacaaaga
    24181 gggagagatt ccctgaatag cagtcaagag aatcgtcata gtaacaccaa aattcttcca
    24241 aacagtcatg atggctaccg tcggcatagc aagggttgga tcgcgaaaaa attgagggat
    24301 cggcaagccg acttgtgaaa acaagtaagg aatcgcccct acattcggat caagcaacat
    24361 agaaaaaata atcgcaattg acgtcaatga acaaataact ggaatgtaaa atgtcgaacg
    24421 acaaaaacga ttgaatctgg tatttttaga taatgctact gctgctaata aaccaacgat
    24481 aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg aattaactgc
    24541 tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc ttgatgtaaa
    24601 gaaaatattc aaatctgtaa aactaatagc aaatgttcct aaaagcggta tgacagtaaa
    24661 taataataaa actaaaaaag atggagttaa aaacaaatac gcagcacgat caggacgatt
    24721 ccaatatttg gtgaatccgt tcatatagtc cacctcatct ttcattttga taaaatatac
    24781 aatcttggtt taaacgaatc tgaataagat atccgaacaa tcgctgtgat ttttgcaata
    24841 acacctatct gcaaatacta ggtcgatcat tcagatatct gtatcttgcc aacagtctat
    24901 cctgtataaa gttctgtact atttttgcag taattgattg atggcatcat ctgcatcgtt
    24961 catgagtgtc tcaggatctt ctcctgctaa taatttttca atcagtggat tgatgatatc
    25021 gttattgatc gcagagattt gggtaaaacc tggtaaatat ggctctgcat agtccatttg
    25081 tttcgataat tcagatacaa taggatcatt ttttacttct tcatcttttg ctactgaatc
    25141 caagtaggca ggaaaaccat tttcgatact ccatttttta ccaatttcag ttgtattcca
    25201 atatttcaaa aactcataga cagcttcttt tttattatta tctgtacttg ccggaatggc
    25261 atatcctacg ccatctaaaa tagctgtttt tttgtctgaa tcactccctt gtggtaaggt
    25321 agtcacatta taatcaatct cgtttgcttt caatccgtta tttaaccatg gcccattgat
    25381 ttcaatggct agttgaccag cattcattaa attgtccatt tcggcgccag aaatcgattc
    25441 tggaccagtc ttgtctacat ggatcatttt ttggatcgct tttaacgttt taaggttctc
    25501 aggagacgca aattcagact tagtataatc atcgttcact aatttaccgc catttgctaa
    25561 catccagtta tataacggtg cagttccatc cttattgaag acaaaacctg atacattttt
    25621 actactatca gttaattttg gtgccatttc tgtcaattct tcccaagtag taggtggttg
    25681 ttcagtatct aatccagctt gtttaaacaa atcattattc caatacatat acatgccttg
    25741 tacctgcatg gggacaaaat actgtttacc ttctacttca cctaattcaa tagccgtttc
    25801 tttaaaatca gatttatcta ctccatcata ttcccagaaa tcgtctaatg gttgtaccgc
    25861 tccgttttct acatactgtg caaaatctgg ataatttaat gcaataaagt caggtgcttt
    25921 tttagaagaa atcgctgtag gaagtttttc attcaaattg gcccaagtca tgatatccat
    25981 ttcaattttg atgtttttat catttgtttt attaaattca ttaacgattt cttgcaatat
    26041 ttcaccatca gatgcagtaa acccattcca aaaagtcaaa gtcacttctt tatcagaatc
    26101 agattctgct gaacttccgc caccgcaagc tcctaaaata cctgttgcag ctaccaacaa
    26161 agagccaatt aaccattttt tacctttcat tttttgttcc tccttcatct gataaacgac
    26221 atctgaccac attccaagat agcggtttca ataaacaatt gattttccca tcttcagctt
    26281 ttacatttgt tgaatgactt ggttttactt gttcactttt aggaagatta gttgcttttg
    26341 tatcaaatcc actcatttca gaaaaatcaa tcactccctc taatgataat tcagacaagt
    26401 gaacagttag attcatttct tcttctccac ggttaactgc aaatatgaca agttctttgt
    26461 catttattac ggctatactt tctaaatacg gcacttcttc aaaatcttcc gtcgcatagt
    26521 ttgctgaacg aatttgtggg cttaacacct ctccttgtcc atacacagca gcttgcataa
    26581 atggataata gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg attggtgcaa
    26641 taacatttac caattgagca agacaagcaa tctttactct atctgcatgt ttcaataaag
    26701 tgattaatag acagcctaat agtaatgcat cttcaaaatt atagttatct tctagtaaag
    26761 gaggagcaat ctgccaaggc gctgcttgct catcttgttc cgctgaatga taccaaatat
    26821 tccattcatc aaaggacagg ttgatctgct tcttactatg tttcttcgcc ttgactgtat
    26881 cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc taaggaacgt gcaagataat
    26941 gagaaagatc atttttttgg ttgccataat attgatgcag agacaaataa tctatttgat
    27001 cgtacgcttg atcaagaact gttaactccc aatcaccaaa tgtaggcatt tgccgattcg
    27061 aacttccgca taaaaccacc tcaattgaat catctacgag tttcatcgcc ttagccgttt
    27121 cctctgctaa ccgtccatat tcttccgctg ttttatgtcc aatctgccaa ggaccgtcca
    27181 tttcattccc taaacaccat gtcttaattc caaaagggct ctcttgacca ttcgaacgcc
    27241 ttaaatcact ccaataagtt cctttaggaa aattacaata ttcgactaaa ttacgagctg
    27301 catctactcc acgagtaccc agattgacag ccatattgat ttcggccccc acatgctgac
    27361 accagttata aaattcatga atcccaactt cattcgtttc aattgtccgc caagctaaat
    27421 ccaaccttct aggtctattt gctatgggcc caatactgtc ttcccaacga aatcctgaca
    27481 caaaatttcc accaggatat ctaatcatgg gaatgttcaa ttcttttacc aagtgaatga
    27541 cgtcttggcg aaatccactt ttattagctg atggatgatc tggctgataa atcccttcat
    27601 atactgctcg tcccatatgt tcaataaatg aactgtacaa ccgcggatca actttgccta
    27661 cacgcttgac actactaatc aacattgtgg cttccataac tgacctcctt taatttatac
    27721 atgaataata cacctcgttg aaagcgttgt caataatata aatgttaaac ttacttattt
    27781 tttatcgatt ttaattaacc aaaatattaa taatgattaa aacaaataat ttaatataac
    27841 ttttattaac aaatatgtta atatataaga aaaacctttt aagaggtgat atatatgcaa
    27901 ctacaaataa acgaagaatc cttaccggta tatgaagcat tagctagtaa aacaaggatc
    27961 aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc aaaagaactt
    28021 ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc aaacatcatc
    28081 aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa aatcgatatc
    28141 agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat acctattggt
    28201 cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca tgattttatc
    28261 gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc ccgcattctc
    28321 tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac agaggataca
    28381 ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc aaatgacaat
    28441 tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg gacgagccct
    28501 ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga taatcttaat
    28561 caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat gaatggagaa
    28621 cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg gcatttgcga
    28681 atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg taaaggattc
    28741 ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt ttcttttcta
    28801 ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc tttattgtta
    28861 tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg tcttgttcca
    28921 ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta gataatcatc
    28981 aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt tcccatcttc
    29041 aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg cttgaagcaa
    29101 ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc caccatcacc
    29161 aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta atacggctcc
    29221 tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt tgatatttcc
    29281 tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt gtgcatcaac
    29341 agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa gagcagcttt
    29401 tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa attgatagtt
    29461 ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac ggcctgctaa
    29521 agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg gatatttttt
    29581 caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat caattgttgg
    29641 tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc ctcctatgct
    29701 tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat tttatggttc
    29761 cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg tttatactct
    29821 tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag tgcaggtaat
    29881 tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc tgtaaagtct
    29941 cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc ttcttcttct
    30001 cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc aaaatttcga
    30061 gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt gcttgataac
    30121 ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt gaatactgca
    30181 ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca tacatcaatt
    30241 ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt cgaagcatag
    30301 atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt agatatatag
    30361 gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc taattctttc
    30421 gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc gactaagaac
    30481 acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt tccataagat
    30541 tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt cccagcacgt
    30601 acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca gactgctgca
    30661 tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc gatgacagat
    30721 ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt caacagtgct
    30781 gtttctacta attggcagtg cgctaaaggc ccttccactt ggattaatgg ttcatttgca
    30841 aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa attttgtaaa
    30901 tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat atcggaatca
    30961 ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa aaccgcgtat
    31021 ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa atcagctcga
    31081 cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg cagcgtcaaa
    31141 ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt tttcatttcc
    31201 tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt ctgaaaaagc
    31261 acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac tatcctttct
    31321 tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat ccgagacacg
    31381 aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt cctactacaa
    31441 tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct acaacataat
    31501 caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct tatccccaaa
    31561 gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta tgacaagata
    31621 taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa gatacaaagt
    31681 aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc agtctcgcct
    31741 tgttttttat tgtatacatt agatatggag caggttacca taaagatgat tattagtcgt
    31801 tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt atttggacgt
    31861 ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac tttgacatct
    31921 tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct tgtgattttt
    31981 ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa tagcagttgt
    32041 gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa aaagaattcc
    32101 actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt ttcgattagc
    32161 ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt tatgttacca
    32221 tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa ggatttacct
    32281 tttatcactt aatagtataa cataatttcg tgagaaaaaa atacaatttt ttcttgacca
    32341 tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg gagtacacaa
    32401 taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt tatgatgaga
    32461 ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat ggcaaaaaag
    32521 aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag ttggatgata
    32581 tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc aagcatcagc
    32641 aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc caacagacac
    32701 ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac
    32761 aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa aaatacggaa
    32821 aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag
    32881 aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag
    32941 aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggttc tcttacagct
    33001 ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac
    33061 ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa
    33121 ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac
    33181 ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc
    33241 cattctttca caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata
    33301 aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca
    33361 agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc
    33421 gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac
    33481 catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg
    33541 ttttcctttc tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa
    33601 aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc
    33661 caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat
    33721 tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa
    33781 aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt
    33841 aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc
    33901 tttatgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat
    33961 gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat
    34021 gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc
    34081 tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat
    34141 tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc
    34201 cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga
    34261 ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc
    34321 caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc
    34381 ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca
    34441 taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct
    34501 ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc
    34561 caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac
    34621 attataatga tactgtaaat atatctttat tattagtgaa t
//
LOCUS       DAYYCQ010000021        33288 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000021 DAYYCQ010000000
VERSION     DAYYCQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.021"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..409
                     /locus_tag="ACHAYF_001051"
     CDS             134..409
                     /locus_tag="ACHAYF_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8578507.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="ACHAYF_001052"
     CDS             415..1182
                     /locus_tag="ACHAYF_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8578508.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="ACHAYF_001053"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="ACHAYF_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8578509.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="ACHAYF_001054"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="ACHAYF_001054"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8578510.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="ACHAYF_001055"
     CDS             5326..5628
                     /locus_tag="ACHAYF_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8578511.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="ACHAYF_001056"
     CDS             5631..6794
                     /locus_tag="ACHAYF_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8578512.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="ACHAYF_001057"
     CDS             6820..10248
                     /locus_tag="ACHAYF_001057"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8578513.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="ACHAYF_001058"
     CDS             10269..11393
                     /locus_tag="ACHAYF_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8578514.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11374..11820
                     /locus_tag="ACHAYF_001059"
     CDS             11374..11820
                     /locus_tag="ACHAYF_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8578515.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="ACHAYF_001060"
     CDS             11804..12142
                     /locus_tag="ACHAYF_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8578516.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="ACHAYF_001061"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="ACHAYF_001061"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8578517.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="ACHAYF_001062"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="ACHAYF_001062"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8578518.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="ACHAYF_001063"
     CDS             13299..14342
                     /locus_tag="ACHAYF_001063"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8578519.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="ACHAYF_001064"
     CDS             14501..15229
                     /locus_tag="ACHAYF_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8578520.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="ACHAYF_001065"
     CDS             15345..15590
                     /locus_tag="ACHAYF_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8578521.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="ACHAYF_001066"
     CDS             15587..15805
                     /locus_tag="ACHAYF_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8578522.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="ACHAYF_001067"
     CDS             15941..16480
                     /locus_tag="ACHAYF_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8578523.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="ACHAYF_001068"
     CDS             16473..18746
                     /locus_tag="ACHAYF_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8578524.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="ACHAYF_001069"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="ACHAYF_001069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8578525.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /gene="rpsT"
                     /locus_tag="ACHAYF_001070"
     CDS             complement(19919..20173)
                     /gene="rpsT"
                     /locus_tag="ACHAYF_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8578526.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20573..20842)
                     /locus_tag="ACHAYF_001071"
     CDS             complement(20573..20842)
                     /locus_tag="ACHAYF_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578527.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21104..21232)
                     /locus_tag="ACHAYF_001072"
     CDS             complement(21104..21232)
                     /locus_tag="ACHAYF_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578528.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(21312..21767)
                     /locus_tag="ACHAYF_001073"
     CDS             complement(21312..21767)
                     /locus_tag="ACHAYF_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8578529.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21905..23833)
                     /locus_tag="ACHAYF_001074"
     CDS             complement(21905..23833)
                     /locus_tag="ACHAYF_001074"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8578530.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(24030..24863)
                     /locus_tag="ACHAYF_001075"
     CDS             complement(24030..24863)
                     /locus_tag="ACHAYF_001075"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8578531.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25055..25759)
                     /locus_tag="ACHAYF_001076"
     CDS             complement(25055..25759)
                     /locus_tag="ACHAYF_001076"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8578532.1"
                     /translation="MTETTLYIVRRGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25782..26408)
                     /locus_tag="ACHAYF_001077"
     CDS             complement(25782..26408)
                     /locus_tag="ACHAYF_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8578533.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26734..29109
                     /locus_tag="ACHAYF_001078"
     CDS             26734..29109
                     /locus_tag="ACHAYF_001078"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8578534.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29133..29624)
                     /locus_tag="ACHAYF_001079"
     CDS             complement(29133..29624)
                     /locus_tag="ACHAYF_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8578535.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29811..30731
                     /locus_tag="ACHAYF_001080"
     CDS             29811..30731
                     /locus_tag="ACHAYF_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8578536.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30885..31229
                     /locus_tag="ACHAYF_001081"
     CDS             30885..31229
                     /locus_tag="ACHAYF_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8578537.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31419..31919)
                     /locus_tag="ACHAYF_001082"
     CDS             complement(31419..31919)
                     /locus_tag="ACHAYF_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8578538.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32447..32530
                     /locus_tag="ACHAYF_001083"
     tRNA            32447..32530
                     /locus_tag="ACHAYF_001083"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32481..32483,aa:Leu,seq:cag)
     gene            complement(32854..>33288)
                     /locus_tag="ACHAYF_001084"
     CDS             complement(32854..>33288)
                     /locus_tag="ACHAYF_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8578539.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11009 a         5772 c         6784 g         9723 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga ggaattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagacg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattatttg tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccatgcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 ttatctattt aaacaaagtt tgaatgggaa tgtttggtac taagttatta tataacaaag
    20461 agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt
    20521 ctattaagaa actgcagtta ttttataacc agtatccttt gatcagtaaa ggctattctg
    20581 gtgtaccgat tattttaatg tattcatcga tattttttat caacattttt tttatgacca
    20641 actggttccg acggttccat ttgccactta cttccagcac atatttattt aggggcaaac
    20701 gtaagatata ttttgccaaa tagcgatctt taagcaggcc attgatcaca cggtcctcaa
    20761 gctgcaagtt gatcccagtc agcatcattg gctgttcttg taaaacgtac gtatcaatta
    20821 aaagaccttc aaaagtttcc acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag
    20881 gcactaattc ttccattttg atattcgttg aagtggatgc gatcattctt tcttttgtcc
    20941 actgtaatct agtataacaa aaaagagcag aagagccaaa aagaaaaaag agcatcgaat
    21001 tctataggaa gtctcattgg cacggcctaa attattacaa aacattgatt aaaaaaagcc
    21061 cctgcatcgc aggagcatcc cctatatcgt tttaataggt caatcaaaaa gcagtatcaa
    21121 acacattttg tatcaaatca ggaaataata gataagctaa gcccagaatc aaaagtaccg
    21181 ctagaagaaa ccagccgatt gttcctatga aatactttaa tttttcgaac attgtatcac
    21241 ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt taagaatctc ctaaaataga
    21301 caaaatttgt tctattttcc taaagcaaat acttgtaaga tacagtaagt gataccagaa
    21361 gcaatgaccg gtccggcggc gattcctctt aagaagacaa cacctaaaat cgtgccgaaa
    21421 acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc ctacaccctt ggaagataag
    21481 acagccacta gcccaccgca aaaaacagct acccagccaa taggggattt aaaggcttct
    21541 aatagatcac gtagaccgat ttctcctgtt gcgataggga ttagaatagc cgctgtgatg
    21601 atcgtcaccc caatattgat tcctttgcct tgaagccaag ggaaaaagga acgggaggct
    21661 ggtatgagct tcataatcaa aacaaaagct gccgctatca tcagactttg atttttagct
    21721 aaaattgcta caagtaaaat aattcctaaa aaaagccaac tttccatgaa atatattcct
    21781 cactttcttc gaaacaatca taacatgttt ttagaaagtt acaaaagtag gaattcatcc
    21841 ttcaagacct attcataagc tgtttttttc ataaaataaa caaaatcata aaaaaacact
    21901 ttgcttattt gacagaagac agccgcgctt tgtatagtgg acaagactta catttttttg
    21961 aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa gccattcaga ataaccaata
    22021 ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt taaaaattgc aggaagttag
    22081 aagcaatcaa caagagaata acaagaatcg aaaaaataat attttgtttg atgatccgcc
    22141 gcatttttaa agatagccga tgactgtaga caaatttatt taagtcgctt ttcattaaga
    22201 cgatgtctgc gatatccata gcggcatctg tcccttgccc catagcgaca cctaaagaag
    22261 cggctgctaa agcaggcgca tcattgactc catcaccaac catcgcattt acttggtaaa
    22321 gttcattctc ttttttgact agttgtgttt tttcttccgg agtacagctt gcatagtaat
    22381 gttcaatccc tacttgttca gcaatggcct ttgctgtgcc agaatggtcg ccggttatca
    22441 tacttgtatg gatgttttgg gatttaaagt aggaaattac ttgttgggta tttgcttttg
    22501 gaatatctag cagacctaaa acagcaatta actgctgatc tttagaaaga taaatcacgg
    22561 ttttgccttg attttccaaa cgttcgatca tttccgcaat ctctttgctg attatcatcg
    22621 tctcaggatc atatgcgtgc ttgccaactt tccattgcgc acctaaatag gtagtttgta
    22681 atccaaaacc agtaatttct tctattggta agtattggat ttcttcaggt attgtgcaat
    22741 cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa
    22801 gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct gtcagttaca actggttttc
    22861 ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt taattcagaa agcttttcta
    22921 aatggatacc gcctttgatt aaaatcccat tttttgcaga atttgaaaga gcagctagag
    22981 aagctggtgt agcagatgcg acaagcgcac aaggagaagc cacaactaat aataccattc
    23041 ctcgataaaa gctttcatcc caactccaac ctaggaaaaa gtgggggaga aggatcatta
    23101 gaggaacagc aagcaaaatc aattttacat aattattttc gattcgttcg ataaatgaag
    23161 ctgtttgagt tggcgtattc tgcgcttctt ctactaattg gataatctta gcaaagacag
    23221 tttcggaact cgtttttgtt actcgaatcg tgatgggatt tccttgattc agcgttcctg
    23281 catacatctc atcgcctaaa tatttttcta caggcacaga ttcaccgctt aatgcagact
    23341 catcgatcgt tgcctgtgtg ctttctaaaa agccatctat cggaacagct gctcctttag
    23401 cgacaaatac caagttatcc acctttagtt gagaaacatc tacttcttgc atttgtccat
    23461 tttcttttaa taattgggct ttttgtggtt gaagattcat caaagcagta atttcttttt
    23521 gacttttatt agttgtgtat tcctctaatg caccgcttaa acaaaagatg aatgtcaaca
    23581 ttgctccttc aaagtaatta ccgatcaaac aggcaccaat agcagctaaa gccatcaata
    23641 aatccacatt cagtgtccga tcattccaca attctttcaa gccttccgat gtttgtttat
    23701 atccaccgat gataatggca ctgatataaa aaagtggggt gatggtaaga gagaatcggt
    23761 cgaagataaa accgaagatc ataaataaaa ggcagccgat tgttgctgca aaagcttttt
    23821 tctgattagt catcgttatt agacctcctt gtcaaaacaa ggatagcata ataaaaatgt
    23881 aattgataat aataacagct aaatgagaac gataatggtt atcatttggt gattaagctt
    23941 gatgttgttt ttattattta taatgattat tattaaagaa aataaaaaac cagaacattt
    24001 ctcaaataag agaagtgttc tggttttttc tacattgcaa taaaccaata accaatcaaa
    24061 atgataccaa ggataatacg ataccagcca aaagcggtaa agtcgttttt cttcaaatag
    24121 cttagtaaga atttgatagc taagacggaa accacaaaag atacgaatgt tcctacaaga
    24181 agaacaacag tttcttccat tttgaatgaa ttcccttcag ctaaatattt gacgatcttc
    24241 aagccgcttg cgccaaacat gacaggaatc cctaagaaaa atgaaaactc tgtagcgaca
    24301 aaacgcgaag caccaatcaa gatagatcct aggatcgttg cgccagaacg ggatgttccg
    24361 ggaacaagtg ccaagacttg gaataaccca acaagaagag ctgcttggta cgtaaagtca
    24421 ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat aataaaggca
    24481 ataccgtaaa cgatcaaaac aatggcaact ggtaagaact tgtggaaatt tgcttctaac
    24541 caatcatcca aaggcaaacc aatgatgatg gctggtatgc aagcaacgat cactttaaac
    24601 caaagtgtcc acgtatcttt tttttctacc gttgatttac ttggagaaaa tgggtttaac
    24661 ttgtgaaaat aaagaacgac gacagccaag atcgccccta attggataac gacattgaac
    24721 atcgacataa attgtgaact ggcatccagt ttgatgaatt catctgctag gatcaaatga
    24781 ccagtactac tgatgggcag ccattccgtt attccttcaa taataccgag aatcactgct
    24841 ttgataatat ttgcaaaaaa cataaattcc ttccttccca attaagatac atcttatagt
    24901 ataccgttat tttttgagaa acccaatctg ttttttaata ttaagaaaaa cttgagaaat
    24961 aaagcagata agtacacgaa gagaaaaaag aagacaaagc cctcgttcag actttgcctt
    25021 ttatgattct ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg cgactatgtg
    25081 ttttcccgtt atcgatataa gacgtatcat tgatgccgtg gatagaaaat cgaccgtttg
    25141 cataagtgac ctttgttacg ctgccattta aaagcccagc acgaacaggc tgtttaggat
    25201 cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa acatttccac
    25261 caccattttt ttcgcagtga tgggcgatat cttcaaatcc tgtccataca cggtctcgca
    25321 atgtttcata agtttccgcc cacccagcgg tatctaagtt gtatacagca ttagcaattt
    25381 gttcaaaaga aacttctgtg gtaaacattt catcatatgt tttaaaattc aatacacgag
    25441 ggatcacacc ccacatttcc atgtcatatc caccttcaag agacccgaaa caccattctc
    25501 taatcctttt ttcaataaaa taaggaattt cttttccctt agaatgttct cctagaataa
    25561 tacgggcagt ttcgattgca cgaccactgt cactagaata agcaaatgaa aagtcgacat
    25621 tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg aggggtgtat
    25681 cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt ttttccgcgt cgtacgatat
    25741 aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt tttaacgatt gaaaaaagga
    25801 tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat gtcctggata gactgcaaat
    25861 tcatcaggta atacaaacag ctgggtccga atactatgaa gtaattgttc taaattccct
    25921 gtatacagat cggttctacc gatacttcct ctaaacaggg cgtcgccaga aacgacaaaa
    25981 tcatcaaaaa taaagctgac gcttcctata gaatgccctg gggtagggac gacgcgaaaa
    26041 gacatgtttc ctaaagtata agttttcatt tcaaattcat attctgcagg cgagacaata
    26101 acatcagcaa tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg atcacctagc
    26161 catttttgct ctagtgggct gacataaact gggatttgat aatacgcgcg taaaggttcc
    26221 actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg ctgttggttt
    26281 gtttttttga tttcttgttt gattcgttcc gcatcttctc cagggtcaat gatcaaaagt
    26341 gcttcttcgt tgtagaccaa ataacagttt tcttcaatca ttcctgtttt taatctttcg
    26401 atatgcatgt gcattcctcc ttgatagcca aaacttcttt catccattag tataacgcat
    26461 tcattgctag gaaacaaaaa caggtactta tttctcttta cttatctgaa ttatcagact
    26521 atatgaaaag tatttggata ttctaaagag aaggaaagta agttttcata gcattttaat
    26581 atgtccttgt tacattagat tcatccaaaa atccaaattc tatatcctat atccctgcct
    26641 aatcagcagg gtgttttttt atgtttggaa agagagctct ttttgctaaa ctaggttttt
    26701 tcaggtaatc ttagagagaa agaggtggtg gaaatgatca aacagtcaaa aatatctgtg
    26761 cggcacttag tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa aaaaagtaac
    26821 catactgcat tagaaggagc gagaatccac cgcaagctcc aaaaagatgc gggagaaaac
    26881 tatgaaaaag aagtcttttt aaaaacaaca gttcaattag atgaatatta cctgatcgtt
    26941 gaaggccgtg cagacggtat tttcaaaaga gacggtattt attatataga tgaaatcaaa
    27001 acctccgaac cacgatttga agatttagaa ccagaacaag tggaactttt ctttcatcaa
    27061 gcaagggtgt atgcctatat ctatagtcaa gaacatgatt tacaagaaat aaatctgcaa
    27121 ttgacttact tccagacggc agaagaagtg atcacgagaa aggttgaaca tcagacaaga
    27181 gagcagttag aaactttttt taaaaagctg actgaagatt ataaagaatg gcttgttttt
    27241 caagaaaact ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt cccccatgat
    27301 gagtatagga aaggtcagcg tgaactagcg gtagctgctt ataaaacgat ccgtacgaaa
    27361 cagaaattgt ttgtagaagc acctaccgga acaggaaaaa caatctctac gttattcccc
    27421 gctttgaaag ctgtagggga agaagaggga gaaaagatct tttatctaac ggctaaaaca
    27481 ataactagac aagtagctga agatgctatg acagcattaa aagatacagg agctgaagta
    27541 aaaagtgtca ctttgacggc gaaagacaag atctgctttt tagatgagac gacctgtaat
    27601 ccagatcagt gcccatatgc caatggttat tacaatcgga ttaatgaagg tttatgggat
    27661 cttttgaatc atgagaatca gatcactcgt gaagtcatcg aaacttatgc aagaaagcat
    27721 acactttgtc ctttcgaact atcattagat gtcagtatct ggtgtgacgt aatcattggg
    27781 gattacaatt atttatttga tccaactgtt tatttaagac gtttttttga agatgaaaaa
    27841 aacgaggact atctgttttt gattgacgaa gcacataatt tggtgaatcg ttcccgagag
    27901 atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc tataccaaag
    27961 gaattcaaaa aacttcatcg ccgtttcaac aaactattaa aagaatttga ttcgatccga
    28021 gaaatagcaa aagaagatca ttgggattat catcatcaaa aagcaccggc tgaatcttta
    28081 gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat ggctggcaga atttcctgag
    28141 cacccatcac aagaacaatt gcttccttat tattttgatt tgcttcattt tttgaaggtc
    28201 agtgaatttt atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg cgatctgata
    28261 gtaaaagagt tttgtattga tccaagcttg tttcttgagc agtcgttgga taaaggaaaa
    28321 agcagtattc ttttttctgc tagtttttca ccactctctt attatcagga aacactgggg
    28381 ggacaaaaaa gtctggctta caaactgccg agtcctttcc cagaaggaaa ccagcaagtt
    28441 ttcatcgcaa attacctaga gacgacttat cgaaaaaggg aacacagtct ggcaaaatta
    28501 gtagagacga ttcatgcttt tgctgatgga aaggtcggga attatttcgt tttctttcct
    28561 tcttatcaat atttagatct tgcggtggaa gcattcagac agcaatatcc cgacagcaat
    28621 gtgctaatcc aagaaacgga catgaatgag gaagaacgcg aacgattttt aacaaaattt
    28681 caaacaaatc ccgctgaaac attgattgga ttttgcgtac ttggcgggat tttttcagaa
    28741 ggaatcgatt tgagagggga ccgattgatc ggcagtatga tcgtaggtgt aggtttgccg
    28801 cagatgaatc atgagcagga actgatcaaa tcatattatg atgagaagga acaccttgga
    28861 tttgcttacg cttatcagct tcctggaatg aacaaagtct tgcaagctgg tggtcgagtg
    28921 attcgtggca tggcggatca aggaatcgtt gtattggcag atcaacgatt ttctagtttt
    28981 tcttatcgac gtttattccc acaacattgg caaacagcaa gagaagtacg aagtgttggg
    29041 gaattggaag aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa tcaaaaaaac
    29101 gaggaatgac agacgtcagc ctcgtttttt gattaacgat agttagtgaa ttgcaactcg
    29161 attggtaagt caagtttacg taataaagca atcacagctt gtagatcatc acggttctta
    29221 cctgtcactc taatctgatc ttcttggatt tgtgctttta ctttcagttt ttcatttttg
    29281 atagctgtag tgattttctt cgcgttttca cgatcgattc cgttgaccaa atcggcagtt
    29341 tgtcgagctt tgccacctag agcgtgttct gattcggaaa aatggatatt tttgattggc
    29401 acatttcttt tgatcagctt accaaataat acatctttga tctgttcgat tttgaaatca
    29461 tcatcgctca atacaactag attgctgttc tctaatttga tttctactgt agaatccttg
    29521 aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa tcgcattttt tacttcttct
    29581 gtattgactt ctgatacaat gtcgaagcta gcatcttttg ccataggaac cctcctgata
    29641 tacatataaa tatttaccat acatctatct tatcatagga ttagggcaga ttcttacctg
    29701 tttgctaaaa ttcttctcat ttgagttcga agtattttat tttaatagat aaactgttat
    29761 tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt atgactgcgt
    29821 atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga ttgaccgctg
    29881 cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg atgaatggga
    29941 tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata gacacaggaa
    30001 catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa gtaggaaaac
    30061 gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg atgctgcttc
    30121 cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt gttttattag
    30181 gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg gatatcatct
    30241 ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg attctaaatg
    30301 gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg tatacgatta
    30361 tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat caaaaagtaa
    30421 cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca aaacggatgg
    30481 tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa gggaacatga
    30541 tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt catgaaatcg
    30601 acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg tttgcaaatg
    30661 aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa aatcgttcac
    30721 aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca tctaataaga
    30781 acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca agcagctaaa
    30841 aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc gaagcaaacc
    30901 aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct gccttggaaa
    30961 tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt tatgatatag
    31021 aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact atgggctgtc
    31081 cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg ccagaagtaa
    31141 cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag atgagccgtt
    31201 atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat gagaagcaaa
    31261 taggaaaaac aaaaaagcct tttatccttt ctagaagaaa agagataaaa ggtttctttg
    31321 tttataaaat tttgtctgct atattataag ttaattaaat aaatgtgttc tattgataaa
    31381 ggacattcaa atccctttcc ttttggaatc cttctttttt acatcagttt gattttcgta
    31441 tagtattctt tctcatcttc acgataaatc gcagccccat ttttttgttg tgctttaaga
    31501 ctgccaagat tttttttatt tacagacaaa agtgcctcat caatgccgat tttttttgcc
    31561 tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt ttttacgata attaggggag
    31621 atgccgtaac caatatggcc agcgccgttt cttaatgttt cgttcagttt atggcgcaaa
    31681 ttaaaaattc ctacatactc atgttcatca ctttctaaaa taaagacggt tgcgggaacg
    31741 taactctcag gaagatcgat tcctaaagac gttttttcgc atttttttac ataatctaaa
    31801 aattcagcaa atgtatagcc ataagcattg ttgataaatc cactctcatt tggtccaaag
    31861 ctagtttgta gctgataagc tttctttaaa tctgcttccc atagtttgat cagtttcatc
    31921 gtattcacct ctttaagtaa ccttattcat ttgttttcta ctatagcact attgttgtaa
    31981 atagccatgc ctatttttat ttattgtgtc atatcgtatt aagaatcatc gaacaataca
    32041 aatatgaatt acaataaaaa aattatttga agttcgttca ttcgttactt atgatctcta
    32101 gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag taatcaacag
    32161 aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag aggaaaaaag
    32221 aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa cattgaaatc
    32281 gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga gttgctcgga
    32341 tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag aacaaaaata
    32401 tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg ctatggcgga
    32461 attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag ttcgagtctt
    32521 cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga tgagaaagaa
    32581 aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc agattcaaat
    32641 aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat tgattcaaat
    32701 agtagattta tagattctga tacaagaaat actggcaaac gatagttaag gaataaagac
    32761 taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct cttactatat
    32821 tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg cttcccagaa
    32881 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    32941 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacggccgat
    33001 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    33061 tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    33121 ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa caggttgttc
    33181 agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
    33241 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DAYYCQ010000022        32387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000022 DAYYCQ010000000
VERSION     DAYYCQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.022"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1016)
                     /gene="ribF"
                     /locus_tag="ACHAYF_001085"
     CDS             complement(75..1016)
                     /gene="ribF"
                     /locus_tag="ACHAYF_001085"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8578540.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(1020..1943)
                     /gene="truB"
                     /locus_tag="ACHAYF_001086"
     CDS             complement(1020..1943)
                     /gene="truB"
                     /locus_tag="ACHAYF_001086"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8578541.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(2311..4673)
                     /locus_tag="ACHAYF_001087"
                     /pseudo
     CDS             complement(2311..4673)
                     /locus_tag="ACHAYF_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            4838..5294
                     /locus_tag="ACHAYF_001088"
                     /pseudo
     CDS             4838..5294
                     /locus_tag="ACHAYF_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(5451..5798)
                     /gene="rbfA"
                     /locus_tag="ACHAYF_001089"
     CDS             complement(5451..5798)
                     /gene="rbfA"
                     /locus_tag="ACHAYF_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8578542.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(5825..8131)
                     /gene="infB"
                     /locus_tag="ACHAYF_001090"
     CDS             complement(5825..8131)
                     /gene="infB"
                     /locus_tag="ACHAYF_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8578543.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(8144..8455)
                     /locus_tag="ACHAYF_001091"
     CDS             complement(8144..8455)
                     /locus_tag="ACHAYF_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8578544.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(8452..8745)
                     /locus_tag="ACHAYF_001092"
     CDS             complement(8452..8745)
                     /locus_tag="ACHAYF_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8578545.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(8767..9942)
                     /gene="nusA"
                     /locus_tag="ACHAYF_001093"
     CDS             complement(8767..9942)
                     /gene="nusA"
                     /locus_tag="ACHAYF_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8578546.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(9965..10438)
                     /gene="rimP"
                     /locus_tag="ACHAYF_001094"
     CDS             complement(9965..10438)
                     /gene="rimP"
                     /locus_tag="ACHAYF_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8578547.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(10583..14941)
                     /locus_tag="ACHAYF_001095"
     CDS             complement(10583..14941)
                     /locus_tag="ACHAYF_001095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8578548.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(15153..16862)
                     /locus_tag="ACHAYF_001096"
     CDS             complement(15153..16862)
                     /locus_tag="ACHAYF_001096"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8578549.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(16930..18198)
                     /gene="rseP"
                     /locus_tag="ACHAYF_001097"
     CDS             complement(16930..18198)
                     /gene="rseP"
                     /locus_tag="ACHAYF_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8578550.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(18359..19159)
                     /locus_tag="ACHAYF_001098"
     CDS             complement(18359..19159)
                     /locus_tag="ACHAYF_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8578551.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(19156..19968)
                     /locus_tag="ACHAYF_001099"
     CDS             complement(19156..19968)
                     /locus_tag="ACHAYF_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8578552.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(20163..20720)
                     /gene="frr"
                     /locus_tag="ACHAYF_001100"
     CDS             complement(20163..20720)
                     /gene="frr"
                     /locus_tag="ACHAYF_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8578553.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(20723..21445)
                     /gene="pyrH"
                     /locus_tag="ACHAYF_001101"
     CDS             complement(20723..21445)
                     /gene="pyrH"
                     /locus_tag="ACHAYF_001101"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8578554.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(21581..22462)
                     /gene="tsf"
                     /locus_tag="ACHAYF_001102"
     CDS             complement(21581..22462)
                     /gene="tsf"
                     /locus_tag="ACHAYF_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8578555.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(22561..23343)
                     /gene="rpsB"
                     /locus_tag="ACHAYF_001103"
     CDS             complement(22561..23343)
                     /gene="rpsB"
                     /locus_tag="ACHAYF_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8578556.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            23702..24181
                     /locus_tag="ACHAYF_001104"
     CDS             23702..24181
                     /locus_tag="ACHAYF_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8578557.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            24398..25489
                     /gene="phnW"
                     /locus_tag="ACHAYF_001105"
     CDS             24398..25489
                     /gene="phnW"
                     /locus_tag="ACHAYF_001105"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8578558.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            25482..25610
                     /locus_tag="ACHAYF_001106"
     CDS             25482..25610
                     /locus_tag="ACHAYF_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8578559.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            25614..26159
                     /locus_tag="ACHAYF_001107"
     CDS             25614..26159
                     /locus_tag="ACHAYF_001107"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8578560.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(26613..28304)
                     /gene="argS"
                     /locus_tag="ACHAYF_001108"
     CDS             complement(26613..28304)
                     /gene="argS"
                     /locus_tag="ACHAYF_001108"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8578561.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(28724..29671)
                     /gene="arcC"
                     /locus_tag="ACHAYF_001109"
     CDS             complement(28724..29671)
                     /gene="arcC"
                     /locus_tag="ACHAYF_001109"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8578562.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(29786..30805)
                     /gene="argF"
                     /locus_tag="ACHAYF_001110"
     CDS             complement(29786..30805)
                     /gene="argF"
                     /locus_tag="ACHAYF_001110"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8578563.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(30896..32125)
                     /gene="arcA"
                     /locus_tag="ACHAYF_001111"
     CDS             complement(30896..32125)
                     /gene="arcA"
                     /locus_tag="ACHAYF_001111"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8578564.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8645 a         6937 c         5735 g        11070 t
ORIGIN      
        1 tcctgggctt atttatcacc cattttattg taattgctag ttatacataa acaagagatt
       61 ttatgcataa tgttttattg ctgagaaaaa tattcagctg ttgcttgttc atcctccttg
      121 agttgttcaa tcaattcctc tacactagaa aatttcactt gatctcttaa aaattgattc
      181 cagcgtaccg cgacatgttc accatagata tcttgattaa agtccaaaat atacaactct
      241 acagtcagct cccgtccttc accgaatgta tcattatgtc caattgaacc catggctgga
      301 taccatgttt ccccaatttt tatttccgac acataaacac cttctttagg caaatgaacc
      361 gtgctcttta ctttgacatt tgctgtaggg aatcctagta gtctgcctcg tgcatcgcca
      421 tggacaacta ccccttctac ctcatatgtg caacctaata gttttgctgc ttcttttact
      481 tgtccgctgt ctagtaatcc acgaatacgg gtagagctga ttttttcacc ttgttcttct
      541 tctttagaaa ctgtgacgat tttgaatctt cctttggcat attcaggaaa atgagcgaca
      601 tctgcaatgt cttttggtcc atacgtataa tcaaaaccgg aaacagcata ttttgcatgt
      661 aaccctacga tgtattgatc aacaaattcc tgcggtgcta aatgagcaaa agaagaagta
      721 aaatcaactt catataaata atctataccc aaagcggcca ttttttcttc tttttgctta
      781 aggctagtca aatatttcac gctgctagga tcgatctttt taaagacaat agatggatgc
      841 tgattgaaag tcatcaatgc taatttcaat ccttcttttt gtgcaatttc catacctgtc
      901 tttatgactt tctggtgtcc gatatgcacg ccgtcgaaaa atcccagcac taaaacgaca
      961 tcgtcttctg gaatttgttc tgattggtaa ggatgtctga tcttgatgat attcattcgt
     1021 tattcctcat ttctcaatac tttactaggt tttaatttgt ttttttcttt cgggtttggc
     1081 tgataaatac tgacaacttt tcctttataa aacaaagcaa tttcttctga cggcatttct
     1141 gggagcgcaa atacagtata atcaagtctc ataccgtttt ttactttctg ccatacttca
     1201 tcagtaatat ctactcgttt gaaccgttcc actccttttt ctattggcag caaacaatca
     1261 tcgattgtcc catttttcac agcttgagct acttcttcta atgtcagagc atccttctgg
     1321 gacataccgg cacttgctgt tcgggtcaga tcagacatgt gcgcagggta accaagtttc
     1381 ttgcctgtat ctacagctaa tgtgcggaca taggttcctt tcccgcattc tacttcgaag
     1441 cgccaagaaa tggttcctgt cacttcgtct cttgtcaatt cacttgtccg gataaaacgg
     1501 taaatctgag cctttctaac aggacgctcc actgtttcat tattgcgggc atattcatat
     1561 aaccgcttac cgttgacttt taccgctgag tacattggtg gtatctgggt gatctcacca
     1621 ataaatgaag ccatcacttc atctattttt ttttcagata aagacatatc tactggtttt
     1681 gtttctaccc gttctccgga tcgatcttcc gtcgttgtac tgaaacctag agttatctcg
     1741 cctacataga ttttcccaga atctgtcaaa tattcgataa cttttgtggc ttttccaata
     1801 caaatgggca acacaccgtc tacgtctggg tcaagcgtac ccccatgtcc gatttttttc
     1861 gtatgtaaga tttttcttaa tttgaatacg caatcatgac ttgtcattcc gcgttctttc
     1921 cataaaggta ataacccgtc catgtctttt ctccatttca tttttgattt aactgaatta
     1981 ttataacaca ttgcatcaat tactgacgaa tttgacatca taatgaacgg tttttatttt
     2041 tcggcaataa aaacgtaaca aaattcatgc tcacgtaaat agaaatcatg ttatgctttt
     2101 ttaaaggagg gaatagaatg gaagattttt tctttgcacc atttgattgg atttatgggt
     2161 ggcttacagg taaatagttt ggttttcact tcccaaaaac gaaaaaaaag actggccatt
     2221 cttccagtct tttttccgtt ttttttattt attttttcag tttcttaatc aatgtaaaaa
     2281 attctatttt gcgggatcga ttttatcatt ttattggtca agatttttcc atttccaatc
     2341 caatacttct ggcagatcaa taccattttc acgaatatag ttttgatgtt cttgtaattt
     2401 ctgatccatt tttttcgcaa aatcttctgc ttgacttcct ttgattttta aagctgcgtc
     2461 ttttgccaaa tgataacgat caagttcatt tactacacgc atgtcaaatg gtgtcgtgat
     2521 atctccattt tctttgtagc catgaatcat taaatgatgg ttatgacgat caaagaaaat
     2581 cgtgcggacc aatgtttcat aaccgtggaa agcaaataag atcggtttgt cttttgtaaa
     2641 atattgatca aattcttcat cgctcaaccc acgcggatct tgacttggat gacgtaattt
     2701 caataaatct acgacattga tgaaccgaat ttttagtttt gggaattctt ccagcagcaa
     2761 agttacagct gctaatgctt ctaagttaga ttccgttcct gcagcagcaa tgaccaattc
     2821 aggttcctcc tcttttgctg tcgaagccca atcaatgatt ttcaagcctt cattcactaa
     2881 gacggctgct tcttctgctg agtaaaattg agcacgcgga tgtttggaag agatgatcaa
     2941 attgattttt tctgacgagc ggaatgcttt atccatgact gcgagcaatg tatttgcatc
     3001 cgcaggcaga tattcacgga tgaactccgc cttcttttct gccaaatgtg tcaatattcc
     3061 tggatcttgg tggctatatc cattgtgatc ttgctggaag actgttgagc ttgcaatgag
     3121 atttagcgaa ggataatcat ttcgccaaga aaggtcatgt gatttccgca tccatttgaa
     3181 gtgctgagtc aacatagagt caaccactcg caagaatgat tcgtaacttg caaaaaacca
     3241 tgccgtccag tcagaacgta cccttctaag aacccttcat cttggtgttc cgataattga
     3301 ctgtcgatga cgcgaccaga tggcgaaagc cattcatcat tttctggttc gattttttcc
     3361 atccattggc gatttgtcgt tttgaaaact tgattcaatc ggtttgattt tgtttcatct
     3421 ggtccaaata tacggaaatt ttctggattt ttctcaatga tctcgcgaac gaatttacca
     3481 agttcggtca tatcttctgc tttaacggag cctggttttt caaattgcaa agtatatttt
     3541 ttccaatctg gcataataag cggtcttgga tcgattccac cattggtgat aggatttttt
     3601 gccattcttt ggtcaccagt aggcgcgatt tctctgattt cttctaaaat acgtccttgt
     3661 gcatcaaata atttttctgg ttcataggat ttcagccaac ggagtaaagc atctgcatga
     3721 tccatatgtt cttgatctac aggaatcggt acttggtgtg ctcggaaggt tccttcaatt
     3781 ggttccccgt cccattcttt tggaccggtc catccttttg gtgtgcgtac gatcaatact
     3841 ggccattttg gcatcacgac atctgttgct gtcttcaggc gagcttcttt ttggatggat
     3901 ttaattttct cgattgcttg atccattttt tcagccataa caggatttaa tttttctgaa
     3961 tcatttcctt caataaagaa aggttcccag ccaagtccgt taaagtaatt tgctaattct
     4021 ccatctgatt tacgtgccaa gatcgttggg ttggctattt ttgcaccatt taaatgaagg
     4081 atcggtaaaa cagctccatc tatcactgga ttgataaaga catttgagaa ccagcttcca
     4141 gctaaaggac cggtttctgc ttcaccatca ccaatcacga cagcagagat tacttcagga
     4201 ttgtctaata cagctccgac tgcatgtgaa agagagtatc caagctctcc gccttcatgg
     4261 atagaacctg gtgtttctgg tgctgcatga gaagcaatgc ctccaggaaa tgaaaatcgt
     4321 ttgaataatt tttgcattcc tgtctcatct tccgttactt ctggatagat ctctgtgtaa
     4381 cttccatcta aataagcatt cgataccatg acttgtccgc cgtgtcctgg cccttcaata
     4441 taaaacatgt tcaaatcata tttattgatg actcggttca aatggacata aataaaattt
     4501 tggccaggaa tcgttcccca atgaccgatt ggatgttttt tgacatcttc gggtttcagt
     4561 gtacgtctca gtaaggggtt atctttaaga taaagttgcc caacagaaag ataattagca
     4621 gcacgccacc aagccgtcat tttatcaaag tattctttac tagaataatc catacataac
     4681 actccttttc attattactc ttattctagc gcagatactc ccaattgaaa cctaataagc
     4741 acttctgttc atttgtttgg attttatatc tagccaaatg aacaggtata aaaaaccacg
     4801 aaatttttcc tttcgctgac gttttttaca ttgatccaaa aaacttgtcg ttatcacaat
     4861 ggctgttccg cagttcattt cgctcctcat catgcataat ttgctgaatg acgctgggcc
     4921 agttaacaca ttgctggaaa actggggctg gatcgagcaa ttcctttcct gattgatccg
     4981 ttttatgtaa aagtctctgt tatctttgtc aatatgtgaa ttggtatccc agctactatg
     5041 ctaatttcta cagggattat ccaaaatttg gatcgtgacc aaatcgaagc ggcgtaatcg
     5101 gaaatatcaa taacttcaac gtgatttttc ttttaactgt caacggaccg acaaactcaa
     5161 acttttatgg tgcaggttcc actgatcttc tagttacttg gctatacctg actgtcacta
     5221 aaatagatta caatctagct tccgttatcg gtatcttgat ctttatctat atgcaaaaat
     5281 ttatatacta ctagaaaaaa gaaagtcgaa ttttttactt ttctttttct cttttttggt
     5341 tagctgacac gccgcaagac taaagtcatg gaactcttag gtaatacgtc ttaatgaaaa
     5401 caaaaaaatc ccgcaacccc tgatttcaca ggatttgcgg gagtttttga ttagtctttg
     5461 ttcaagtcgc gaagcaattc atcgatatgg ttgccataga caactgattc atcaagttca
     5521 aagaacaatt caggtgtctt ataaatactt agattgtgcc ctaactctcg acgaatcaat
     5581 cctgatgctt tattcaatcc atcttgtgct gttttcttat ctgatgctaa gtcagataaa
     5641 atgctgtaat agatcgttgc ttgctgtaag tcccccgtaa ctttcacatc tgtgatcgtt
     5701 acgttctcca cacgagggtc acggaccttt ttgcgcaaaa tatcattgac ttcttttaag
     5761 atttcttgac cgactcggcg gtcacggtag ttagccataa tgattggcct ccttttatca
     5821 gtttttagtc atttttgact tcttccatga taaatccttc aattacgtca tctactttga
     5881 tgtcattgaa tttttcaatc attgcaccac attcataacc cattttgact tctttcacgt
     5941 catctttgaa tcgtttcaga cttgcaagtt ggccttcata gattacgatt ccgtcacgga
     6001 ttacacggac accgctatct ctacgaatat atccttctgt tacataggca cctgcaatcg
     6061 taccaacttt agaaactttg aaggtttcac gaacgatcat ttgaccggtg attttttctt
     6121 caaattcagg gtctagcatc cctttcatcg cagtttcgat ttcttcgatg gctttataga
     6181 tgatacggtg aagacgaata tcgacttctt cagtatcagc ttgcactttt gcctgcggtg
     6241 taggacgaac gttgaaaccg atgataatag cgttgctggc tgctgctaag gttacatcac
     6301 tttcattgat tgctccgaca gctgaatgga cgatttttac acggacacct tccacatcaa
     6361 tcttcttcaa tgatgcagcc agtgcttctg ctgaaccttg tacatctgct ttgatgatta
     6421 cgttaacttc tttcaattcg ccttctttca ggctttcaaa taagttatcc aaagtcacac
     6481 ggcttgtggc agcacggttt tctaataatg cacgtttcgc gcgttcttca cccgctgcac
     6541 gtgctgtctt ttcgtcttcg aagactacga aacggtcacc tgcttgaggt acatcattca
     6601 agccagtgat ttctactggc gttgcaggtc ctgcttcttt atcgcgacgg ccaagatcgt
     6661 ttgtcattac acggacacgt ccgtaagtat taccgacaac gattgggtca ccaacatgaa
     6721 gtgttccttc ttggacaagt aaggttgaca caggaccttt acctttatca agacgtgctt
     6781 cgatcactgt tccgattgcg cgttgtgttg gatctgcttt taaatcttct acttctgcga
     6841 ctaacaggat catttccagc aattcttcga tgttttggcc gaatttcgct gagatctcaa
     6901 caaagatcgt ttctccaccc catgcttcag gaattaactc atactcactt aattcttgca
     6961 taacatgttg agggtttgca cctggtttat caattttatt tacagcgacg atgattggaa
     7021 cgcctgcagc tttggcatgg ttgattgctt caactgtttg tggcatgacg ccatcatctg
     7081 cagcaacaac taagatcgtg atatctgtga tacttgctcc acgtgcacgc atacttgtaa
     7141 aggccgcatg tcctggtgta tccaagaaag tgattggttt accatcaata tcgatctggt
     7201 aggcaccgat atgctgtgtg attccgccgg cttcgccgct tgttacacgt gaatggcgta
     7261 atgtatccaa taaagttgtt ttcccatggt ctacgtgtcc cataatcgtt acgactggcg
     7321 gacgtgagac taatttatct ggatcgactt cttctgcctc gaagaattta tcgatatcag
     7381 caatgtcaac ttggactttt tcttgtggtt ccatgccata gtccgtcgct aacagttcaa
     7441 tcgtatcttt atccagtgct tggttttgat tgaccatgac accaagcata aataattttt
     7501 taatgatttc tgctggctca cgatgaattt ttttcgcaat atctgctacg ttcatacctt
     7561 cagtatactc taatacatct ggcaattcac ggaatttgcg tggtggtaca gctggtttag
     7621 ttgattgttg ctgtttgcct tttttacctt ttttattata acgattacgg ttattattat
     7681 taaatttgcc gcgattgtta ttgtttggtt tgttgttatt gttagtacgg ttttgattgc
     7741 cgccttggct cgttccttga cgattttggc tgctggtatt tcccgtatta ttttggtttg
     7801 ttgttggttt ttttccttga ttcacttgac cgctcccttg accgcgatct tgataactgc
     7861 ggttattttt atttttttgt tgattgttcg ttgtttcttt tttacgttga tcgttcggct
     7921 gttgattggc tggttttctg cttgcttgtt gtgcagcttg ttgtttcgtg cttgggtttt
     7981 tgaacgcttg ttgcaatttc ttctcatcat tgctagtaat cgcacccata tggtttttca
     8041 catcgatgcc aagttgttgt gctttttcca cgacgtcttt actagatttt ttgagttctt
     8101 ttgctaattc atagattctt ttgttgccca tgcaatcacc ttcctaacct tcaattagct
     8161 ccttggtctt cttcgcaaat ccggcatcca ttattccgaa taccttgcga ggttttccga
     8221 tcatctgcgt gatttctgct tcagaaaata gctcaaagca aggaacttca taatatgaac
     8281 tcttatcttt tatctttttt cttgtattct cgcttgcatc ggttgccaca aacaccaatt
     8341 ttgctttttg tttgcggatt tcagcaatgg tcagttcttc acccgtgatt agttttcccg
     8401 cgcgcatcgc tagaccgatg agattcatgg ctttttgccg attaactgta ttcattttcc
     8461 aaataactct ttacgtgctt tttggtgagt gacatagtca agaagttctt gataaaactc
     8521 atctgttaat gttgtttcga gtactcgatc gagaattttt ttatcccaag cttgttgtac
     8581 ttcttccggc tcaagtgaga catacgctcc tcgtccaggc atttttccag ttggatcgat
     8641 cgatacttcc ccttcttttg aacgggtgat tcggaccatc tcttttttag gtttcatttc
     8701 acctgaaacc acggatttgc gtaaaggaat tttcctttgt ttcatctctt tgcctcctta
     8761 ctaaagttaa gcttctggtt tttcttcgac cgtttcattg ttgaatgcaa ttgtttcata
     8821 ttcatcgtct gttaaatctg actgaacgat tgcttcatca tgcagttctt ctgtttcttt
     8881 ttgttgttct tgattttcat aaaattccgt catgtcagat tctgatttga tatcgatttt
     8941 atggttagtc aatttagcag ctaaacgtgc gttttgtccg cgtttaccga ttgctagcga
     9001 taattggtag tctggaacga caactgtgca ggcctttgga tttgtctcat cgaagatcac
     9061 atccatgact tgtgcaggat tcaatgcatt tgcaatgaat acagctggat cttcattcca
     9121 ttcgacgata tccatgtttt cgccttttaa ttcattgacg attgcttgaa ctcgttgtcc
     9181 ctttggcccg acacatgtac cgactgcatc gatatttgga tcatttgagc gaactgccac
     9241 ttttgaacga tcgccagctt cacgggcgat gctgacaatt tcaacgattc catcatagac
     9301 ttctggtact tcttgctcaa ataagcggcg caataggtct ggatggctgc ggctaacaaa
     9361 tacttgcgga cctttagacg tattttctac tcgggaaaca taaactttga ttcgatcatg
     9421 tggttgataa aattcatttg gcatttgatc ttgtttagaa agaactgcct caattttacc
     9481 aagattgaca taaatataac gtttatcctg gcgttcgacg attccttgca tgatgtcttt
     9541 ttcataagca ctgaattcgt tataaataat cgttctttct gcttcacgta cacgctgtaa
     9601 aataacttgt ttagccgttt gcgcagcgat ccgcccaaaa tcttttggtg ttacttcaaa
     9661 acggatcgta tcaccgatct catatgctgg attgattaaa agtgcatctt tcaatgagac
     9721 ttccaactgt gaatccatca cttcttccgt tacttctttt accgcataga catggatctt
     9781 tccttttttc tgatcaaatt ccacttcgac gttttgtgct tggccatagt gacgtttgta
     9841 tgcagaaacc aatgctgctt ctaaagcatc gatcacgatt tcttttgaga tccctttttc
     9901 agcttctaaa gcgtctaacg catttaacat ttctttactc attttttctg attcctctcc
     9961 gtgattaaaa ttggatagct aagcgagctt ttgcaatatt ttttcggtca aaagtcaatt
    10021 ctttttctcg tgtttttatg cgtattttca atgtaagctg ttcagcatca aatgattgaa
    10081 gaaatccttc atattgtttt tcgccatcta ctggttggta taaggagaca tggacataat
    10141 caccaagtgc cttttcatag tcgctttctt ttttcaatgg ccgctctgct cccggggagg
    10201 atacctccag gaaataagct tgaggaatcg gatctggatc tgctgcatca agtttttcac
    10261 tcagtttctc actgacaaag gcgcattctt cgatatcgat accgccttct ttgtcaataa
    10321 atactcgtaa gaaccagctt tttccttctt ttacaaattc gacttcgaca agttcaaaat
    10381 tttgttcatc taaaatcggt gtcaccattt ctgtgactgt ttcaacgacg ctactcaaaa
    10441 gtttcgcctc cttaaaaaat taagtgcgta attccattaa aaagagtgag cgaaaaatcc
    10501 gctcactcac agttagtatc attacctata agaactatac catactctgc ttaaaattac
    10561 aagcagacag gaaaacagat gcttatagca tatcgaataa cgataattga ttttcatctg
    10621 gtagatcttt caatacgcca ttgtcgttca tatattcgat caatgttttt gaaactttac
    10681 cgcgggtggc aagatcttct ttggataaaa atggaccgtc ttttcttgct tcaacgattt
    10741 gttttgccac gttggttccc aaacttggga cagctctaaa tggtgcgatc aacgtatcac
    10801 cttcaataac aaagttaacg gcatccgatt tatacaaatc aatcattcca aatttgaatc
    10861 ctcgttccag catttcattg gctaactcca gcactgtcag ttgattcttt tcttttacgg
    10921 aagcatctaa tcctttgtcc gtgatttctt tcattgcttg tttcacagca tcttttcctt
    10981 tgcacatgga gactagatca aagtcgtctg cacgtacaga aaagtatgca cagtaataca
    11041 aaatagggaa ataaaccttg aaataagcga cacgaagagc catcaatacg taagctgcag
    11101 catgggcttt cggaaacata tatttgattt ttgaacaaga atcgatatac cagtcgggca
    11161 cattattttc tttcatcgcc gatagatagg tttctcgcaa ttcatcaggg atcttgttcc
    11221 attgtccttt acgaacagtt tccatgattt taaatgccat cccgctatct aatccagcat
    11281 ggatcaaata aaccataata tcatcacgac agccgattac ttccgccagt gtcgcatctc
    11341 ctcgtcgaat caattcttcc gcatttccta accatacgtc tgtaccatga gatagtccgg
    11401 aaatttgcag tagttctgca aaagttgtag gatgcgtttc ttctagcatc cctcgaacaa
    11461 aacgagtgcc gaattctgga atacctaatg tccctgtttt ggaataaatt tgttcctgac
    11521 ttactccaag tacttcagga ccggcaaata tccgcatcac ttccgggtca tccgtaggga
    11581 tcgtttgtgg atcgatccct gataaatctt gaagcatccg aataacagtt gggtcatcat
    11641 gccctagaat atctaatttc aatacattat cgtgaatcga gtgaaaatca aaatgagtcg
    11701 ttttccattc cgaattttga tcgtctgctg gatattggat cggcgtaaaa tcatagacat
    11761 ccatataatc gggaataacg atgatccccc ctggatgctg gccggttgtc cgtttgactc
    11821 cagtagcgcc ttttgctaat ctgtcgactt ctgcacttcg aaactgcaga ttattgtccc
    11881 tttcgtatcc ttttacatag ccatatgctg ttttgtcggc gactgtacca atcgttccgg
    11941 cacggtaaac atagtcttcg ccaaacaata ctttcgtata gttatgggct tctgcttgat
    12001 aatcacctga gaagttcaaa tcaatatcgg gcactttgtc tccatggaat cctaaaaatg
    12061 tctcaaatgg aatatcatga ccgtctttgt ttaagcgtgc accacatttt ggacattgtt
    12121 tttctggcat gtcaaatcca gaaccatacg taccatcttc aaaaaactcg gaatattggc
    12181 actctggaca ataataatga ggggctaacg gattaacttc agtgattcct gtcatcgtcg
    12241 caacaaagct agagccaaca gagccacgtg aacctactag atagccatct tcattacttt
    12301 tatgcactag tttttgggcg atcagataaa taaccgagaa gccattacca ttaatagaat
    12361 tcaactcttt ttttaatcgt ttttgaatga tctcaggtaa cggatctccg tacatttgtt
    12421 ttgcttttgt gtaactcaat tctgatattt catcttctga acctggaatc ttcggtgtat
    12481 aaagttcatc tttgactgga atcacttctt cgcagatatc tgcgatttta ttcgtatttt
    12541 ctactacgat ctctttcgct gtttcttccc ctaaaaagtg aaatgcagtt agcatctcat
    12601 cggttgttcg gaaatggaca tccggcaaac tatgacgatt caacggattc gctccgccca
    12661 tagaattgat caaaattttt cggtaaatgg catcttcttc gttcagataa tgtacattac
    12721 ctgttgcaac aacgatttta tccaaacttt ttccgatttc taccaaattt tggataattt
    12781 cttccaaatc atgttcattt tttaccaatt cttgctcgat caaaggcgca tagaccgctt
    12841 ttggcataac ttcaatataa tcgtagaatt ttgctcggtt tcttgcctct tctacccctt
    12901 tttgcatcat ggcttcgaat atttctccct tatcacaagc tgagccgatc aatagatttt
    12961 cacgcatctt tttcaattgg gagcgtggta ttcgtgggac gcgttcgaag tattcgacat
    13021 tcgacattga aatcagtttg aacaaatctt tcagtcctgc ctgatttttt gctaaaatcg
    13081 ttacatggaa aggtcgagcc cttttgtaag aatctccttc tccgatatgc tcatttagct
    13141 gatcatgata aagcatgtta tgattgtcca ttgcctcttt cacaaagatc catgctaagt
    13201 gaccagttgc ttcagcatca tagattgctc ggtgatgctg ttccaagctg acaccaaatt
    13261 ttttagataa cacacctaat ccgaaacgtt tgaattgagg atacagatat ctagctaatt
    13321 ccaacgtatc aatgactgga ttggccgctt caggaatacc ataccttgcg tagctagtat
    13381 tcaagaaccc catatcaaaa gccgcgttgt gggctaccaa aattgtatct tttgaaaatt
    13441 caaggaacat ccgcaatact tcttcttccg actttgatcc tcgaaccatt ccatccgtga
    13501 taccagtcag atctaccgtt gttcgtgaca aaggatgtcc tggatcgata aattcatcaa
    13561 agctttcgat cacatttcct ttatacattt tgactgcggc tagctcgatg atcgtgtcat
    13621 atactgcaga caatcctgtc gtttccacgt caaatacgac atatgtggct tcactcaatg
    13681 cttcatgtgc atcattataa gcaattggga caccatcatc tacgacattc gcttccacgc
    13741 cgtataagat tttcacacct gctttttttc cagcactatg tgcttccgga aaggcttgcg
    13801 caccaccgtg gtctgtaatg gcgatagctc ggtgtcccca tttccctgct tgtgccacaa
    13861 gatcagagat gctgtttgtt gcatccatcg tactcatatt gctatgaaca tgaagctcta
    13921 ctcgtttttc cccttcagga gcataatctt tgcgaggtgt atgtttgact tctatgatat
    13981 cttgcgcatt catgaccaaa tcacggacaa acgtatcttc ttgaatactt cctcgtacct
    14041 ttaaccagct accgacactg atcgcatcaa atacttgttc atctttttcg ccgttcgaga
    14101 attttttgac gataaatgat gaagtataat ctgtgatttt caaagtcaag atcttgcgtt
    14161 ttgaacgtag ttctctgact tctttgtcga atacgtaccc ctcaagtgtg actcttcgtt
    14221 cttcttcaac gatactgatc attggtgtcg ttggttcatc ggctggaata tttcgtccta
    14281 gctggatgtg gatgggtcct tcaagagctg gcgattgctc ttttctttcc tttttctttt
    14341 gttcgtggac aactagactt tcagctgctt gcttcataaa ggcttctgcc tgttcttgtt
    14401 tgcgttcttc aaaaagtttc aatactcgtt ccgcttgctg ctcatccact tctggttcga
    14461 tacgaaattt tgggaatccg taactgacga acaactcttc tactaatggc aagtattgtt
    14521 gcttcaaata agaaatagct cctgtactgt cgattggcaa gatgacttta cgatctttta
    14581 tcacaggtac ctgcgttttc aatacttgct gtgctagtgg tgtatcgcat tgctgatttt
    14641 ccaaagcttg ggcccagtaa tcttgcaaga gctgttcatc gaatgtctgg tcatctgcta
    14701 agatattcag tttgacttgt gcaatatctt taaaagcgat agtcagatgc tgagtcaatg
    14761 accgatatag cataataggc aaaattttgg aaaaatgcag cgtaaattcc cataaacgcg
    14821 atttacgatg gaccactaca ctatccattt caccagcagt aagcagtgga tgattttttt
    14881 ctgcttcctc tagttggatt tgttccaaca gtttttcaaa taaatctcgt ttttctgaca
    14941 aagatatacg ccctttctga tgagaaacaa tgcaattgat cgcatcttat cctcttcttt
    15001 ttcagtacct tttagtatac tcgttttttc tgagttttac attaaaaaat tggaactgcg
    15061 attcaaatcg cagttccaat tttaaaagcg aatcatcaga ttgtcaaaaa cagctgattc
    15121 agctattttt attttctgtg ttttaacgct tattattcgc cttggttcaa gaggattggt
    15181 aaagtattca ccaattcttc tttacgtact tcaaccattt cgccagtttt cttgattttc
    15241 acttctacga ttccatccac tgctttttta ccgacagtga ttctgattgg acagccaatc
    15301 aaatcagaat ccgcgaattt cactcctgcg cgttcatttc tgtcatcaac cagtacttca
    15361 tagccagctt ttgtcatcgc ttcttctagt tcattcgtca ggtttgtttg gtattcgtct
    15421 tttagattca tttggacgat atgcaaatca aatggcgcaa ttccttttgg ccagttgatt
    15481 ccttgttcat ctgaatgttg ttctacgatt gctgataata aacgacttac accgatacca
    15541 taacagccca taatcacaaa tttttctcgt ccattttcgt ctagtacagt tgctcccata
    15601 gattcactgt aacgagtgcc gattttgaag atatgtccga tttcgattcc tcgagtaaag
    15661 gcaagaacac cattgttgtc tggtgaagga tctccttctt gtacaaaacg aagatcttca
    15721 taagaaagta cagtgaaatc gcgttctgga ttcacatttt tatagtggaa tcctgtttcg
    15781 ttcgctccag taatggcatt cgctaaatct tggacataac ggtctgcata tacttttacg
    15841 tcatctgaaa caccgactgg tccgacagaa ccaaactcag cacctagata tttttgtgct
    15901 tcttcttctg ttgcttcttc taaaaagtca gcagataaaa agtttttcag ttttacgtca
    15961 ttgacttcat ggtctccgcg taccaaaaca aggacaggtt gttcatccgc aatgaacaat
    16021 acagacttga tgattttttg aggttcaaca gaaaagaaat ctgctacttc gtcaattgat
    16081 tttacgttag gtgtagctac tttctccaat tcaagctgtg tttcatgcga tttttttgat
    16141 acatagtagc ttgtagccat ctccaagttt gcagcataat cactttcagt cgaataacag
    16201 atcgtatcct ccccgatttc tgagatcgcc atgaattctt ttgagtcttt cccgcccatt
    16261 gcgccaccgt ctccgataat cgagcgaaat tctaagccac aacgcttgaa tacttcggtg
    16321 taggcttttt cgtaatcttt atatgtctcg tccaaacttt cttccgttgc atggaaagaa
    16381 tacgcatctt tcatgataaa ctctcttccg cgaagcaaac cgaagcgagg tcgtttttcg
    16441 tcacgatatt taggttggat ttgataaaga tttaaaggta atttcttata tgaattgatt
    16501 tcattacgca tcaaatcagt aaaagtttct tcgtgagttg gtccaaggat catttttcga
    16561 tcattgcggt ctttgaaacg atacaagttt ggtccatatg tttcgtaacg cccggattct
    16621 tcccaaagtt ctgctggcaa gatggcaggc atcagcattt ctaccgctcc gattttttca
    16681 aattcttccc gcataatgcg tttcagtttt tccaatacac gatttgctaa tggcagatat
    16741 gaataaatac ccgctgatac ttggcggata tatccagctc ttagcaagat ttgatggctt
    16801 aatacttctg catcatttgg aacttcgcgt aatgttggaa tcaacatttt tgattgtctc
    16861 attctatata ctcctttatt tcctcaaaat agttatcgtt tgttattata ccctatttgt
    16921 caactttttc tagaaaaaga atcgttgaat atcgttccat gtaactagca ccatcaacaa
    16981 cattaaaaat ccaaagccag ctaatgtcaa tattccttct ttttcttgac ttaatggttt
    17041 cccgcgaatc ccttcaaaaa tattcaagac taatttcccg ccatctaagg caggaatcgg
    17101 aagcagattg acgattccga gattcattga aagaagcgcc atcaaactaa taacagttgt
    17161 tacaccttga tttgctgctt ctgaagacaa ttggtacatc atgacaggtc cgcctaattt
    17221 atctaaactg aaacctgtaa atagtgatcc taaggctttg aaaatttcca atgaaccgct
    17281 gaaagcttga cgcgtccctc caatgatctt atccataaaa cctgtattca ttggggcttt
    17341 tataccaagt tggccaactt tttcgccatt tgactctaca gatttcggtg tcacgtcgac
    17401 agattgcacc tgaccttctc gttcgatctt aaagtctaat gttttatccg gatttttcgt
    17461 aatgactgtt gtcagatcat tccatgaatg gatttctttt ccgtccacac tgactacctc
    17521 atcattttct tttaagcctg cttctgccgc agctccatta ggcatgatct ctcctacgcg
    17581 attagtgttc gtcacttgaa caccaccttg cataaatgcc agaatgatga ataacaggat
    17641 tgccaaaata aagttattca tgggtccagc aaagttggtc agcatacgct gccacaattt
    17701 agccgattgg aactgtacgt cttttggcgc aattctgatt tctgttccgt cgttttcaat
    17761 aatcgtcgca tcgtgatcaa cagggtaggt tactacttct gtttcgtctc cattcacata
    17821 accagtgata gtcagctcat cttctaaatc ataacggctc aattccatcg ggatagcatt
    17881 tgtcaattgg attttcttac ttaaatttat cttctctacg atgccgtcag aatttagtaa
    17941 aagagaaaga ggcattcctg gtgccatttc tgtttcatca tccccattgc ctgccattcg
    18001 aacataaccg ccaataggta ataggcgaag cgtatatgtt gtcccgtctt ttgcttgatg
    18061 cccataaatt ttcggaccca taccgatagc aaattcgcga acgaggattc ctgatcgttt
    18121 cgcaaagaag aaatgaccaa actcatgaac aatcactaat ataccaaaaa cgatgataaa
    18181 tgtcagaatc gttttcatag cttttttctc ctttacttgt tttttgtgtt ttttataaaa
    18241 caacgataaa acactataat tttaccatag tataccaaag acaccaatag tatcaatata
    18301 atgaaaaata aaaacctgcg tcttttatga aattcacaaa gaaacgcagg tatacttact
    18361 aaataatgcc aaataaatgc atgataggaa atacaaacag caaactgtcg aaacggtcta
    18421 agattccacc atggcctggt aagatatttc ccgagtcttt cacatcataa tgacgtttga
    18481 ttgcagattc gaccaaatca ccaaattgtc cgactgctga aaaaataatc gtcatcagaa
    18541 gcatgacaaa cataccatga ttaaatagtt cttttgctgg gtatagcaat aaataaagaa
    18601 atgcgactac aacagcacta ccaataccac cgagcgcacc ttcaatcgtt ttgttgggcg
    18661 aaatatcagg acttaactta tgctgaccat aacgtcgacc aaccatatat gctccgatat
    18721 cagttgccca aacaataaat agaccaaata tcaaaacagc caagctttct gttcgtgcat
    18781 tgacaaaatt ctggaaacct attcccacat acaagctgac aagcacagga aaacctgctt
    18841 catcaatcgt atacgtattt ttagaaataa ctgatgttcc taaaagaatc ataactataa
    18901 gataaaataa cataaatgta cttacattct ctggaagaaa gaaaaaccat ctttcttttg
    18961 gcaaaacaag taacactgca ccaacggcgg ataaaactcc ttcaaaactt aagagtgtca
    19021 atcctttcat ccgaaacagc tcgtaaactc ctattactgc taagattgct gctgtaagct
    19081 caaccgctat gcctccgatc catataatcg gaataaacaa gactagcgcc acggctgctg
    19141 tgaccacacg ttgtttcatg actgatcccc tttctcatct tctgtttttt ttacaccgcc
    19201 gaatcgacgg tctcgatttt gataagaggc gattgcttct tcaagatgcg caccatcaaa
    19261 atcaggccaa agcgcttttg taaaatacag ctcactatat gcgatctgcc ataacagaaa
    19321 attactaatt cgctcttctc cacttgtacg gatcatcagc tcaggatctt gtaactcttt
    19381 aggcaagaaa cctgtcatta aatgcttagc aatcaattca tcatcgattt cttctgcgtg
    19441 tatttctcct tttgctactt cttcggcaat ttcttggaca gcagtcacga tctctgctct
    19501 gcttccataa ttcaaagcaa aattcaatac cattccagta ttgttttttg tttgctcaat
    19561 tgcacgtctt acagcatctt gcgtatgttc tggcaatacg ttttcataac ccatgacatg
    19621 aactttgaca ttttctttaa tcaattctgg tacgaacgta tcaaagaagt cgacaggtag
    19681 ttgcatgaga aaacttactt catcttttgg tcgcttccag ttttcagtcg aaaaggcata
    19741 aagcgtcaac actttcactc caagccttga tgcttttttt gtgactttct ttactgtttc
    19801 catgccttct ttatggccag caactcgtgg caaacgacgg ttttgcgccc aacgaccatt
    19861 gccgtccatg atgatggcaa tgtgctgagg aattggtcca tcggtattaa attgaaattc
    19921 gcttttttct tgaatatatt tgtttttttg cggaaaaaaa cgtaacattc tttttcctcc
    19981 tatacttaaa agattcccac tatcttaagt attatagcaa tatctaactg tttttcctat
    20041 gaaatcattg tttttttatt taaaaactga ataatttttt ctttttttaa tttttaaata
    20101 aaagaaatat aaataaaaac agaggaagcg gaatgatccg cccctctgtt tttatgattt
    20161 tattaaactt ccaataattc tttttcttta tcagcagcga tcttatctac ttctttgatg
    20221 ctgttatctg tcaatgtttg tacgtctttt tctaatccgc gcaaatcatc ttcggtaata
    20281 tcaccatttt tttgttgttt tttatattca tcgatcgcat cacgacggat gttacgaatc
    20341 gcgatttttg cattttctgc ttctttttta acatctttag ctaattcttt tctgcgttct
    20401 tctgtcagtt gcgggataac taagcgaatg acatttccat cattggttgg actgattccg
    20461 atatcacttg ccataatagc tttttcaaca tcttgaagaa tgctcttgtc aaaaggtgtg
    20521 atcattaaaa cacgtgcttc tggaatttgg attgatgcca tttggttcaa tggtgttggc
    20581 gcaccgtagt agttcacagt aatacggtcc aaaaggctag cgttcgcacg gcctgcgcgg
    20641 atttgcccaa gttcacgttg aagattttgt gctgcttttt tcattttttc ttttgcttct
    20701 gtcataattg catctgccat ttttatttcc cccttacagt tgttccgata ttttcgccta
    20761 agattgcacg gcgaatattt ccgtgttcat ttaaattgaa gacaagcagt ggaatatcat
    20821 tatccatgct tagtgaactt gcagttgaat ccatgacttg taatcctttg gcaataactt
    20881 ccagatgagt cagttcttca aattttactg catttgcatc tactttcgga tcagcagaat
    20941 aaacaccgtc tacattgttt ttagccatca ggataacatc tgcgccgatt tctgccgcgc
    21001 gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc ggtaccgcct gcaaagataa
    21061 cgatccgtcc tttttctaaa tgacgttctg ctttacggcg aatatatggc tctgcgattt
    21121 gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc cacgttttcc aacgtatcct
    21181 gtaaagctaa ggcattcata actgtagcta acatacccat gtaatcagct tgagcacgtt
    21241 ccatgcccat ttgtgctccg atttgtccgc gccagatatt tccgccgccc acaacgattg
    21301 ccatttcaac gcccagctcg tgaacttctt tgatttcttg gatgatttct ttgattactg
    21361 gtggtttgat tccaaatcct gcgtccccag ctaaagcttc tccactcaat tttaagacta
    21421 cacgctgata tttcggtgtg accatgttca ttcctccaat attttctatt gccattttac
    21481 catacttaga tgatttcgac actctaagac tggtttttct tgttttgctt aaaaaaaggg
    21541 agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa ttattttttc acttggctca
    21601 taacttcttc tacaaagtta tcttcgcgtt tttcgatacc ttcgccaact tcgaaacgta
    21661 cgaatgtttt aacagttgcg ccttttgatg caacgtattt ttcaacagtc atatcaggat
    21721 ctttaacgaa tggttggtcc accaaagcga tttctgcttt gaatttcttc aagcgtcctt
    21781 ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt caatgcttgt tctgtaagaa
    21841 cagttttttc atgttctaat tcagcttctg gaatttgtga ttcgtttaca taacgaggat
    21901 tgatagctgc tacgtgcata gctacgtctc ttgcaactgt ttcgtcagtt gttccatcta
    21961 gaacagtcaa tacggcaata cgtccgccca tatgcaagta tccaccaaat gcagcattgt
    22021 cgtctttttc tacaacttca aagcgacgga agctgatttt ttctccgata acttgagttg
    22081 cttcgatcaa gtcagattca attgtacctt tatctgtttt gattttcatt gcagcttcca
    22141 tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc ttttactaaa tcttggaaca
    22201 tttcgttttt agaaacaaag tctgtttctg agtttacttc aacgattgct gcagtgttac
    22261 ctttcacagc aactgaagcc aatccttcag ctgcaatgcg gtcatttttc ttcgcagcct
    22321 ttgccattcc tttttcacgt aaaagatcaa ctgctttttc catgtcgcct tctacttcaa
    22381 ctaatgcttt tttcgcatcc atcattccta cgccagtcat ttcgcgtagt tcttttacca
    22441 ttttagctgt aacgtctgcc atgtttaact cctcctgtgt gtttttcttt ttaaaaaaag
    22501 ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga aacagctcca tcattcaaaa
    22561 ttattctgct gaagaattgt cgccttcaac aacatctacg atttcttcga ttgatgttgc
    22621 tgtgttttct tctgtgaaat cttcttcaac tacttgatct tcaccttggt ttccttcgat
    22681 gaaagcatca gccatttttg aagtaatcaa tttaacggca cgaattgcat cgtcgtttga
    22741 agggattact acgtcgattt catctggatc gcagtttgta tcaaccatag ctacgattgg
    22801 gatatttaat ttgtgtgctt cttgaacagc aatacgttct ttacgtgggt caacgatgta
    22861 cattacatct gggattcttg gcatatcagc gataccgcct aagaattttt caagacgttc
    22921 acgttctttg ttcaaaccag caacttcttt tttaggtaga acttcaaaag ttccatcttc
    22981 ttccattgca ttgatttgtt tcaaacggct gatacgtttt tggatagtat cccagtttgt
    23041 caatgttccg cctaaccaac gatggttaac gaagtattgt cctgcacgag ttgcttcttc
    23101 tttgattgct tcttgtgctt gtttttttgt acctacgaat aatgctacgc cgccttcttc
    23161 agcaacgctc ttcatgtaat cataagctgc atctactaat ttaactgttt tttgcaagtc
    23221 aatgatgtag attccgtttc tttctgtgaa gatatatttc ttcatttttg gattccagcg
    23281 acgagtttgg tgaccaaagt gtacgccggc ttctagtaat tgtttcatag aaattactgc
    23341 catgtttgtt tcctccgatt tggttttatt tttcctcttc ttatcttcta ccttgccggc
    23401 aaactttttc aagcacctac cgacaatcca ataagaatgt ggatttgctg atgcataaag
    23461 caccctcagc tattatacag acaaaagatc cgtatcgcaa gagcaaatat acttttctgc
    23521 tcaacattca atagtttacc tgaaagataa aaagaattca agaaaaacag tagatttctc
    23581 tttgtttctt tattatcctt tttccataaa ataattttag gtataaaaga cgaatattat
    23641 tgggcaatcc ttcttttttt tgttatgata catgtgaaat ctttcgtaaa ggagaaagaa
    23701 aatgcgcaaa gctcaaagac atttgctgat taaacaagtc attgaagaat tcccgatccg
    23761 tacgcaagaa gaactattaa acaaacttgg gcattatggc gttaccgcca cgcaggcaac
    23821 gatttctcga gatatccgtg atctaaaaat cgtgaaggca cctgatgaaa atggcgtttc
    23881 ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact aggactgagg aagaggatcg
    23941 gctggttcaa atgattgaag acattgtatt gaaagtcgaa cgagtccact ttttgacgat
    24001 cgtcaatacc ttgccagaca atgctcattt atttgcatct gttttagatg agatcaaacc
    24061 tccctctatt gtcagtacaa ttgctggatt cgatacgatc atggtcatct cggaaaatga
    24121 ggaggacgca aaacgagtag aagaatttct tcaccatcca actgaagtat cactttttta
    24181 atcagagcac gtattcctaa tatggaatgc gtgttttttt attttcaata ttttccttat
    24241 tgaataaacc tttacactct ttacaaaaat acatcatttt ttattcaaat ttttcaacac
    24301 cttgattgaa tcacaataat catcatttat attggatgtt aacaagagca gcttcttgct
    24361 ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg tataaattat taacttctgg
    24421 accattgacg acttctgaaa cagtgaaaaa tgctatgctg atcgatcatt gtacttggga
    24481 tcaagaatac aaagaaatta cacaatggat aagaaaaaaa ttattggatc ttgctcaagt
    24541 atcggaagga atatacacaa cggtcttaat gcaaggtagc agctccttcg gggtagaatc
    24601 ggtattaagt tctgtgatca ggccagaaga tactgtactg atttgttcaa acggcgcata
    24661 tagacaacga atgatcaaca tgtgcgaatg tctgaaactg aactataccg tttatgaggt
    24721 atcggaacat caaatcctcc aagctcatgt gattacacag ctgattgaag agaatccgtc
    24781 tatctccgca ctagcaatgg tccatagcga gactacttcc ggtattttga ataatttgga
    24841 agagattgct gctgtcacac aagaaagaga tcttttgttt atagtggacg cgatgtcaag
    24901 ctttggtggg ataccgattt gtgtagccga actagggatt gattttttga tttccagctc
    24961 aaacaaatgt attcaaggtg ttccaggctt ttcttttgta cttgctaata aagcggaact
    25021 ggaaaaacga aaaaatgtta gccgaagttt gtcacttgat ctatatgacc agtaccgcgt
    25081 gatggaaaac gagaatggca aatggcgttt cacttcccct acccatacgg ttttggcttt
    25141 tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc gaaactcgat attcacgtta
    25201 tctcgccaat aatcatgtgc tacgacaaaa aatgacagag atggggttcc agcctttcat
    25261 tcaagaatat caggggccat tcatcacctc ttttctttac cctaaggact ggcgatttga
    25321 ttttaatcat ttttatcagt atttaaagca aaatggattt gtgatctatc ccgggaaaat
    25381 cagcgatcat gaatgttttc gtatcggaaa tattggcgag atctatccgg aagatatttt
    25441 ggaactgaca gcaattatca aagactataa ggagaacgtt aatgattaaa actgtaattt
    25501 ttgattgggc aggaacaact gtggactttg gctgtatggc tcctgtccac gcttttagaa
    25561 atgcttttct agaaaaagga atccaactga ctgacaaaga aatccgctaa ccgatgggaa
    25621 aattaaaatg ggatcatatc caacagcttc tttccctccc ttctgtcaaa aaacaatgga
    25681 tagctattta tggacaacta cctcaggaaa aagaggtgga ggaattgtat caacaatttg
    25741 aacattatct gtttaaagat ttggtcaagc attctacctt gaaacccgac acattggaaa
    25801 cgatcgaacg gttgagaaac gctgggatca atattggttc cacgaatgga tttaatgctc
    25861 ctatgatgac aatcgtagca gaaaccgcaa aaaaagaaag gtattcacca gacttcatgg
    25921 caacttttga agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc cataaaaaca
    25981 tgcagtactt caaaaacaag tcggttgatg aagtaatcaa agtcggagat acggtctcag
    26041 atatccagga agggaaaaat gccggagtgt tgacagtagg tgtaattgaa ggtagttcgc
    26101 taatggggat ttcttatgat gagtaccaaa aattagagat ggggaaaaag atcgtttaaa
    26161 acagtctgtt gctcttagat tccaacaagc cggcgctgat atttgtgtaa acaatttaaa
    26221 agagttaaca gaatacattt tgtacgctga catgaatcca gaatgacttt tatacaaaat
    26281 aagaggttgt gaaaaagctg tcctgtatca agtaataaaa acagccaagc aaaaatagct
    26341 cctcatattt ttcgtttggt taggcttatt accgcagatg caagcttttg aacaccgttt
    26401 attcgaaaaa aaaaagggag ctgtgacacg acttttgtca cagtcccctt atttgcaaac
    26461 aaacagtgtt taagagacaa cctctccact aaacggtgtt cattcagctg actctcggta
    26521 tttgctcaaa aaacgataaa atttgaaaag ctattttctc ttttgtttct ctaatactcg
    26581 gatcagaacg ctgatttcac attctctttt tcttacatct tatctggtgc atgaagtcct
    26641 agcaaacgaa ggtcttcttt caataataca gtcactgcat aaactagagc taagcgtgct
    26701 tctttttgtt catcatcagc taaaatcttc gtatgagcat agtatttgtt gaatgcttgt
    26761 gctaatttga ttgcatgttt cgcaatcact gacggttcgt atttttcccc agcagacaag
    26821 accgtttcag ggtatttttg aactaattta accacttccc agctggtatc gtcattcaat
    26881 gcatagtctg ctgtttctga agggacaaat cctgcttttt ccaacaggct gactgcacga
    26941 gcatgtgtat attgcacata aggtccagtt tctccttcaa atcgaacaac ttcttccagg
    27001 ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac tgccccaacc
    27061 ccaacttgtt tagctacagc atctttgttt tccaaatcag ggtttttctc gctgatttgt
    27121 tctttagcag attggattgc ttcatttaat acttcttcta ataagacgat ctttccttta
    27181 cgagttgata attttttacc accttgcgtg atcaaaccaa atggaatatg gtgcatgtcg
    27241 tcagaccagt caaagcccat ttccttcaag acagctttca actgtttaaa atgatagctt
    27301 tgttcattcc ctactacata tagagattgt ttgaagtcgt aagtacgttt acggtacaat
    27361 gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt aatcaacgct
    27421 ggattcaggt cataagcaga taggtcaacg atctctgcac ctttatcctc gttcagcaaa
    27481 tgtttttctt ctaaaagttt gacgatttca tccatcttgt cattgtagaa agcttcaccg
    27541 ttcaacgaat cgaaacgtac ttccagcaga tcatagattt tattgaattc tttcatcgat
    27601 tcatcacgga accattgcca taattgaata gcctctttat cgccttcttc cagacgtttg
    27661 aaccaagcac gagcttcttc atttaattct ggttctgttt cagctacttc atggaattgg
    27721 acgtataaac gaagcaattc attgattggt tctgctttaa cagcttcttc tgtaccccat
    27781 tttttgtaag cgacgatcag ttttccaaac tgtgttcccc agtcacctaa atggttgatg
    27841 cggattggct gataaccgat tttttccata atgaaaccga tcgaattacc gataactgtc
    27901 gaacgcaaat gtcccattga gattggtttg gcaatattag gagaagacat gtcgattggt
    27961 actgttcctt gattcccgat gttgctatcc ccataatgtt ctttttcttt cactactgtt
    28021 tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa agttcaagta aggtccgaca
    28081 acttcgattt tttcaaagtt ggcagaatcg attttttctg ctaaatctgc agcgatttgc
    28141 tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac atctccgtgt
    28201 tcagctgatt ttggattttc tagtaattgc tcgacttgtt ccagtgttaa atcatctttg
    28261 acagcgtcat agacagcttt tgcgacaaga tctttattat tcatctttat tcctcctaaa
    28321 gtgttccctt gattgttaaa caaaaaaaat ctctagcgac ggcatactta gtatgccatc
    28381 actagagacg agatttcccg cggtaccact ccaattgtcc taaaaaagga ccagctccat
    28441 ctgatatcgg tagacgaccg tctgtttaaa acagaattct ccaagtgcgc ttcgttgtat
    28501 cttcttcatt cggcttccac cacttccgaa ttcgctagcc aagaatgata caagtactct
    28561 cttgttcaac aaatcttagt cttattaaac tttcttgaat tatatcagaa aaaaaaacga
    28621 aaggctagtc gtttcataaa aaaacaatcc agatggccta aaggagaaga aataccacct
    28681 ggatagctat aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa
    28741 tcgttccttc ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct
    28801 tagcgtttgg ttttgcttca acaaatgcaa tagctgcttc tactttcgga agcatgcttc
    28861 ctggagcgaa ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta
    28921 atttcttttg atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga
    28981 tcagcaaatc cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta
    29041 cggcttctcg tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa
    29101 cagagactgt gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg
    29161 agatgggttt aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg
    29221 tcacagtgga gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga
    29281 ctggtttact tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta
    29341 aagaaaccac atctttatcg atcccttttt ccttcagtac ttcgcccatg gcgttttgta
    29401 accagtaacc gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg
    29461 ttttctcaga atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc
    29521 cgtgggagat gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt
    29581 ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt agacaagatg gcattgcccc
    29641 ctaaagcgac aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga
    29701 attttctaga taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt
    29761 tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg
    29821 ctgccatgat cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat
    29881 atttgctgcg gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca
    29941 tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca
    30001 aacgatctgt gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca
    30061 atttgatacg ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa
    30121 ggacgtctgc tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg
    30181 atttttcagc aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg
    30241 gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca
    30301 tattgtttcg accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt
    30361 cttggatagt taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat
    30421 tccaaactgg aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac
    30481 ggaattcgat tccgtcaaac atacgtccta gtactttagc tgtatcttct gtggattctt
    30541 ttttacctaa ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg
    30601 ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat
    30661 tttttccttc tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg
    30721 aaaaatcgat caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc
    30781 ttccttggaa tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag
    30841 attaaagagg aaatcccgac ggcagctgac agtaagactg ccgtcaggaa acaaattact
    30901 tcaagtcttc acggacaagt ggttggctca tgcagcgtgg accaccacga cctcgtgaca
    30961 attcacttga attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata
    31021 cataatttcg gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat
    31081 cattccattg ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa
    31141 gatcatctaa tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct
    31201 tgatatcatc accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa
    31261 ttgctggatg gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca
    31321 tcattgcacg gttgttcgga attttgattg ccaacacttt ttcaaatcct gaattttttg
    31381 cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa
    31441 cagctactac tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt
    31501 cacgatccaa ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat
    31561 attctgtaaa catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc
    31621 catttcccat tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt
    31681 aaaatggata gtcatcgtct gctgacagat catatagact gctagaacgt acttcgatat
    31741 cttttgtacg aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa
    31801 ggaactcatg aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt
    31861 tattcaagaa ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca
    31921 agtaaagagt ctcgacgcct tcattttgca atgtcttcgc aaagttgtca tgttcttctt
    31981 gagcaatcgg aagatatgga atatcatcga acaacagacg atccataata tctggtgtca
    32041 aattttccac ttcttgtcca ggccgtttca gtaaaactgt ctttaatttc cctatttcag
    32101 agaaaacgtg aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata
    32161 ggatatcctc cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct
    32221 cacattaata ttcgataatt aaataattaa acgtttttct atttttaagc gtaatttatt
    32281 cattttttat tctgtataca ctttcaaaaa aatatttatt cattgaaaaa atttttttga
    32341 ataaattaaa aaaataaatt atattatcgt tgttagcgtt tactata
//
LOCUS       DAYYCQ010000023        31831 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000023 DAYYCQ010000000
VERSION     DAYYCQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.023"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..379
                     /locus_tag="ACHAYF_001112"
     CDS             <1..379
                     /locus_tag="ACHAYF_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8578565.1"
                     /translation="HRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTE
                     GFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIA
                     FGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(762..1490)
                     /locus_tag="ACHAYF_001113"
     CDS             complement(762..1490)
                     /locus_tag="ACHAYF_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578566.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1468..3042)
                     /locus_tag="ACHAYF_001114"
     CDS             complement(1468..3042)
                     /locus_tag="ACHAYF_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8578567.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3271..4209
                     /locus_tag="ACHAYF_001115"
     CDS             3271..4209
                     /locus_tag="ACHAYF_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578568.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4221..5132
                     /locus_tag="ACHAYF_001116"
     CDS             4221..5132
                     /locus_tag="ACHAYF_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578569.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            5156..6628
                     /locus_tag="ACHAYF_001117"
     CDS             5156..6628
                     /locus_tag="ACHAYF_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8578570.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6679..7290
                     /locus_tag="ACHAYF_001118"
     CDS             6679..7290
                     /locus_tag="ACHAYF_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8578571.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7320..9233
                     /locus_tag="ACHAYF_001119"
     CDS             7320..9233
                     /locus_tag="ACHAYF_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8578572.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9361..12342
                     /locus_tag="ACHAYF_001120"
     CDS             9361..12342
                     /locus_tag="ACHAYF_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8578573.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12339..14000
                     /locus_tag="ACHAYF_001121"
     CDS             12339..14000
                     /locus_tag="ACHAYF_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8578574.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14040..14972
                     /locus_tag="ACHAYF_001122"
     CDS             14040..14972
                     /locus_tag="ACHAYF_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8578575.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(15364..16911)
                     /locus_tag="ACHAYF_001123"
     CDS             complement(15364..16911)
                     /locus_tag="ACHAYF_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8578576.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(17013..17366)
                     /gene="tnpB"
                     /locus_tag="ACHAYF_001124"
     CDS             complement(17013..17366)
                     /gene="tnpB"
                     /locus_tag="ACHAYF_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8578577.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(17356..17550)
                     /locus_tag="ACHAYF_001125"
     CDS             complement(17356..17550)
                     /locus_tag="ACHAYF_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578578.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17776..18978)
                     /locus_tag="ACHAYF_001126"
     CDS             complement(17776..18978)
                     /locus_tag="ACHAYF_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8578579.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18981..19175)
                     /locus_tag="ACHAYF_001127"
     CDS             complement(18981..19175)
                     /locus_tag="ACHAYF_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8578580.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(19222..20070)
                     /locus_tag="ACHAYF_001128"
     CDS             complement(19222..20070)
                     /locus_tag="ACHAYF_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8578581.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(20275..20625)
                     /locus_tag="ACHAYF_001129"
     CDS             complement(20275..20625)
                     /locus_tag="ACHAYF_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578582.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20803..21666
                     /locus_tag="ACHAYF_001130"
     CDS             20803..21666
                     /locus_tag="ACHAYF_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8578583.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            22290..23282
                     /locus_tag="ACHAYF_001131"
     CDS             22290..23282
                     /locus_tag="ACHAYF_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578584.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            23298..24182
                     /locus_tag="ACHAYF_001132"
     CDS             23298..24182
                     /locus_tag="ACHAYF_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578585.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            24231..25838
                     /locus_tag="ACHAYF_001133"
     CDS             24231..25838
                     /locus_tag="ACHAYF_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8578586.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25839..27323
                     /locus_tag="ACHAYF_001134"
     CDS             25839..27323
                     /locus_tag="ACHAYF_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8578587.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27500..28492
                     /locus_tag="ACHAYF_001135"
     CDS             27500..28492
                     /locus_tag="ACHAYF_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8578588.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28544..29413
                     /locus_tag="ACHAYF_001136"
     CDS             28544..29413
                     /locus_tag="ACHAYF_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8578589.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(29833..31446)
                     /gene="guaA"
                     /locus_tag="ACHAYF_001137"
     CDS             complement(29833..31446)
                     /gene="guaA"
                     /locus_tag="ACHAYF_001137"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8578590.1"
                     /translation="MRRTERAAYRTKFYSNLSSPTRFDEEPKIIVLDYGSQYNQLITR
                     RIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVL
                     GICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVT
                     QAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTG
                     DWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVL
                     LRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDE
                     ATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLF
                     KDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANA
                     GLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKI
                     SVRIVNEVAHVNRIVYDITSKPPATVEWE"
     gene            complement(31453..>31831)
                     /locus_tag="ACHAYF_001138"
     CDS             complement(31453..>31831)
                     /locus_tag="ACHAYF_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8578591.1"
                     /translation="HRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTE
                     GFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIA
                     FGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         9972 a         5327 c         6417 g        10115 t
ORIGIN      
        1 tcatcgactg tttaaacgac caattagtca aacagaaatc gttgatgagc tgttaagcta
       61 caacgaagaa ttgcgaactg cgtatcattt ttgtcaatta ttacgctatt atttcgttaa
      121 acgaggtacg gaaggctttc aagaaaccct taaaacaatt tcatctacgt tacctcagtc
      181 gtttaaaaag aaattcacga tcttccattg ctaccaatcg ggtatttcaa atgcctttaa
      241 ggtgtcccac tcaaacggcg tcactgaggg gctgaataac aagatcaagc tgattaaacg
      301 aattgcgttt ggttatcgca acttttataa ctttcgggca cggatctatt tacaacaagg
      361 tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat atcggactaa
      421 gttttattca aatttgtctt caccaacacg atttgacgaa gagcctattt aaataactac
      481 atccatttat tctatcttct aaaatagcct ttttcatttc agttaaatag ttcagcgcca
      541 tttttgcact cgacttgcat agtagttttt tccatattcc tcaacctcag cgggggttga
      601 agacataagg aaaatcctat gcgttatgct caactggatc tgattaaata ttctaatgta
      661 tttttctcta ccatcattaa atggttaata gccaaaaatc accacttttc aatggctcct
      721 tgttgtataa gtataactag aagatgaaca cgtctgagaa attatagaag ctttttacgg
      781 taattactgg ggctgtgtcc agtagttttt ttgaagagaa cagaaaaata gctcacgtga
      841 tctattccta ctttttgact aatttcatca atagataaga atgtgtcttt cagtagagca
      901 cttgctcttt tgattcgaaa gtctagcaag taatcatgaa aggaataacc tgtttctttt
      961 ttgaaggccc gtcctaaata agcgggatgg taatggaatt gtgtagctac atctttgagc
     1021 gtaagctttt cactataatt gttttgaacg taatcaatca cttgttgaat tgactcatta
     1081 aaagaatcat cagttagaga gttttgatta gctgctgatt tacgcatttt ttcttcttgt
     1141 actaacttaa ctaaataagg aaaatcaatg ggtttcgtta tgtaactttt aatagataaa
     1201 tcaatcccct tttttgcata ctgaaaattt tggtgggcac taatgacaat actagtaaag
     1261 tagcgattgg atgtttgttg caaagattcg attaattcga gtccagaaat tttccccatc
     1321 gtaatatctg tgataagtaa atcaaagcgc tggttttgca ggtttttaaa tgcttcgtct
     1381 ccgtctgtgt agcagtgatc gataatgatg tcggggactt tcttcaaaag ataatgcctt
     1441 atgccttcta caattgtaca atcatcgtca acaattgcaa ttctcaacat gcatattccc
     1501 ccctttttgt attttgatag taatcgttgt tccgacgtgt agttggcttt ctataacaaa
     1561 acgtgtatct ccaggataga gatgctttat acgtcgaaaa gaatttgaga tgccgatatt
     1621 ctcactataa ggaatatcat cattaagttt ttgttgaata tgctccagct tgtcttggtc
     1681 cattcctttc ccattttcaa cgatttttaa cacataatgg ttggttgttt cagttgcata
     1741 gacagtcaaa cgattttcct tttttgatga atcgaagccg tgtttaatat aattttcaac
     1801 aaatggctgt aaagaaagtt ttggtaacat taattgatca atatgatcaa tcagctgata
     1861 gtcaaagtta tgtcgataac gacgttgatg tagttgcaag gagaggtcga tattaattaa
     1921 ttcctctttc aaatcaatta tgtcgggctg atatgtatta ttgcgataca acttgctcaa
     1981 taaatatata ctgtcgctga tttcttcgtc atcattcaat atggcagtca ttcgaataaa
     2041 ttccaaatta ttaaatagaa aatgaggctc gatttgatat tgaagtgctg catactttgc
     2101 ttcttgcttc aaccaattat tttcataaag tgttttttga tatgtagcat tatctaaata
     2161 aaactgattg atatgcatag cgatttcttt caaatcaccg actttgtttt cgatattgat
     2221 tgacttgtag tctcctgtag atgaatgatt taaggaagta atgatttcct gatactgctg
     2281 gatatagtta cgaaaacttc tatttaataa ttgatgaata gcaagaataa acaaactcat
     2341 taataagaaa atcatgattg ccagacctaa taaggtttgt gacgaataat ttggattttc
     2401 atatacttgt aatgtgtggt taccgatagc tatctgttgc gtttttttcc atttagaagg
     2461 cgagatgtct tcagataaat tgacggaatc aataccatta gacaaagaat aattgtaatg
     2521 tgttggaaag agactctttt ttagcgcgtt gatcgaaacg tctagaatca atgtagcatc
     2581 gggcatgtcc tcactaatga aacttaatga acgaataaaa gaaatttttt cgttttttgt
     2641 aggtgcgtag gcacttaagt atttttcgta atctaatgag atagatatat ttttaacttc
     2701 atcatcttgc acaagttttt ttaagttatc tactaaatag gtaggggagc gatcctttga
     2761 caaaatatct tccatgtatt cagcaggtga tgagaggtag tagtcattaa ggttttcttg
     2821 gaaatcataa ttttgataga tctctgtcac gataccggag agaatacgat tatatgtttc
     2881 catatagtgg gtgatttgct gaatgttttg caggttttga tttttttctt tttcaatcac
     2941 attgatatag gaaaagaaca atgtgaaaca ggcaagtaaa gtaataaaaa aaatcaatat
     3001 gtatgtaaaa cgcataataa cttttgataa atcgtttttc attcaaagtc ctcattttga
     3061 taatctctaa ttttatgaat cattatagca catattacaa gtgatgtaaa cgctttgcaa
     3121 acagggctac ttatgattgt gaatacagtt tccgtttaaa aactattcta ttgtagaggt
     3181 ttaataaact aaaaataatg tataaaaaat taatgtaagc gattaaccaa gtgggtataa
     3241 tcaaggaaac gctgagagag gagagacaaa atgaagctaa agaaagggtt attaggaaat
     3301 ctaaaaaaaa accgagcatt gtttattatg cttttaccag gtgcgttatg gttatttata
     3361 ttttgctata taccaatgtt tggagttgta atcgctttta aagatttcag aatttcatca
     3421 aggggattct ttacaagttt gttcaacagt ccttgggttg gctgggaaaa tttcaaatat
     3481 ctctttgctt cgcaagatgc ttggatcatt actcgcaata cggttgttta taacgttgta
     3541 ggtattttgc taagtacttt ttgtacagtg tcattagcaa tcattctgag ccaaatggcg
     3601 aacaaacggt tattgaaaaa gatgcagaca tttatgattt ttcctaattt tttatcatgg
     3661 gtagtcatca gttactttgt ttacgcattt ttgagtacgg ataaaggatt aattaatatt
     3721 ttactaagca attttggtgt ggaacccata aattggtata gtgaaccgaa gtactggcct
     3781 tttattattg tttttacgag tttatggaaa ggcgtaggct atggatccat catttattac
     3841 gcgtcaatta tggggatcga tactagttat tatgaagctg ctttagttga tggtgccagt
     3901 aaatggcaac agataaggaa tgtagtcttg ccacatttaa gatcgattat tgtcattatg
     3961 ttgattttgt ctgttggtag tatttttcga gctgattttg gattgtttta tcaattgcca
     4021 agaaattcgg gggctcttta tgatgtgacg aatgtattag atacttatgt ctaccgtgga
     4081 ttaatgaata gcggagatat cggaatgtct acggcagcgg gattatatca gtcatcagtt
     4141 ggctttgtct tgattttgat tactaataag atagtcaaca agttagatcc tagttcagca
     4201 ctattttagg aggaatcaaa atgatatcaa aattaaaacg gcagatgtac ttttcgctgc
     4261 cagtcgaacg gtttttcaca atattactaa tcttatttac tcttagctgt atttttccct
     4321 ttattttcgt aatcgtcatt tcattaacag atgaaaaagc attacaactc aacggttacg
     4381 aattattgcc ggctcaatgg agcactgatg catatcaata cttaatacaa gatggcggac
     4441 agttacttcg gtcattaggc gttacaatta tgattacagt gattggaacg ttgattactg
     4501 tttttatgac gggaacctat gcttatgttt tatcaagagc ctctttccct taccggaagt
     4561 tctttacttt ttatcttttt tttaccatgt tatttgcggg agggatggtt ccttcttact
     4621 tagtgatgac gaagatgctt ggcttaaaaa atacaatttg ggctttgatt ctacctcttg
     4681 cttttagccc atacaatgtc attattttaa ggacattctt taagaagtcg attccagaat
     4741 cgattatcga atccgctaaa atggatggct gtagtgagtt tcgagttttt ttccaaatcg
     4801 ttttgccttt agcgatccca ggggttgcaa caataggttt attttcaagc ttaggttatt
     4861 ggaatgactg gttcaacgca ttgctgtata ttgacaccaa caaattgatt ccattgcaat
     4921 atttgttaat gaagattcaa aattcaatgg aattcttagc aaataataat gacattacat
     4981 tagcgcagca acaagcaatt caaaatagcc ttcctcaaga atcaacacga atggcaatgg
     5041 tcgtagtagc tactttaccg attgcaattg tttatccgtt cttccaaaaa tactttgttc
     5101 aaggattgac gatcggtgga gtgaaagaat aaaaaagaat aggagatgtt aaaagatgaa
     5161 aaagaacatg cagaaatatt tgattggtgg tgcttctgta ctaacccttg gcctattagg
     5221 tgcttgtggt agcagtaatg gcgaagcaaa taatgaaaca ggaaccacaa aagttacttg
     5281 gtatcaggtt ggagaagaac ccaaagattt tgatgaggtc atgacaaaag tcaacgaaaa
     5341 attaaaggaa gaaatcaatg tagagttgga tatgcggctg attccggacg gggattatca
     5401 gcaaaagctt ggtgtgatga tcaattcagg agaagaatat gatatttgtt tcgtcaatgg
     5461 cacagattat gtgaattatg ggaacaaagg tgctttcatt agtctcaacg atcatttgga
     5521 taaagagttg aaagattatg cagctgaatt gaatgaagga tttattgaag gtggggcaat
     5581 taatggcgaa acttatggat tgccagtcaa taacgatatt gccacaacgg aatattggac
     5641 ctttaataaa gatttggtag atcaatacca attggatgta gagaatgttg aaggtgtaga
     5701 aacattggct ccagtattag aaaagtttgc agaaaaaaat actgatacga atattacgcc
     5761 aatcggtatg acccgtacca gtgtctgtat tccttatgac aatattatcg gtggtggtgt
     5821 accgctagcg gttgcatttg aaggcgatac acaaacagtt gttaatactt atgagactcc
     5881 agaaatgatg acttatttga aggctatgca tgagttttat aaaaaaggat atattttgaa
     5941 agatgcagcc actgttacga cagatccatt tcgaattgat agtaaaaact ggtttttagg
     6001 tcgtaactca agtggttatc aaccattagc acaaaaaaaa ttagaagctc tagcgggaca
     6061 tggattaatt ttaaaaccag ctggaaaagc ttttaaaacg actggatttg caagagtggc
     6121 aatgcatgcg gtatctgctt caagtaaaca tccggaagca gccttgaaat tgttgaatta
     6181 tgtgaatacc aatcaagaag tagcggattt aatggtttat ggaattgaag gaaaacacta
     6241 tgatcgcaat gaagatggtt cgattacttt aacaaactct gatgattatt atcaaggcgc
     6301 ttgggcattg gtaaatactt atctagtttc aaaaacttca ccgattacag aagaaagtaa
     6361 aaaactagaa gaagaaagta aaaagtttca aaatgaagca ccgatgtctc ctattttagg
     6421 tttctctttt gacgcttctg attttcgaac agaaattgca agtgtacaaa atatcgtgtc
     6481 acaatatgaa gtacttttaa gctcgggaac agttgaccct gaacaatatg taccagagtt
     6541 gttagaaaaa ctagaaaaag ctggattgga taaaattcag gcatctgctc aagaacaata
     6601 cgatgaatgg cgaaaatcta acaaataatt ttttactaaa tgaaaagaat agactaagca
     6661 gttagtgggg ggattcaaat gaacgaaaac aaatggacca aaggatttaa ttgggttttt
     6721 caattgttgt tactaaactt tacttatttt atactaattt gtttaggatt tggaatttta
     6781 ggttttttcc ctgctacgat agctttgatt gctggcgttg tgaatcgaaa aaaatatggc
     6841 ttgcaagagt ctgtgaagtt catgagtaaa atctataaga aaaattttgt tagatataat
     6901 ctatattgct ggtcagcaac tttgttgttg gccggcaata ttactacgat ctggctttgt
     6961 tggcagcaaa gcgcaccatt gttttattta ttaagtacat tattatcggt aattacactg
     7021 ttcctagtct ttgcaatgtt gaattgggtc gtgatcaaaa gtcttgggtt gtggatgatg
     7081 ggagaccgac tgattgacta tttgcttttc acagcaacga tgttgccaac ggtagtcatg
     7141 caaggcttgg tgcttggaat ttatttccga atattgatga ttttgcctgt gatttttctg
     7201 tttggcggaa cagctgtaac ggtaatattg attagtaaat taactgaaag agcgttagca
     7261 aagttgcctt gtcagataca atcgcactaa ttttttcaca aaaaggagta tttctttcta
     7321 tgaagttaac tgataaacaa gtagctaata tagcctttat ccagcaactt gagattccat
     7381 tgacacaaaa agatgaagaa caaatagact tgatttttac tgacacaaag gctaatgtgc
     7441 gacaaatcaa gattactaaa aatgagcagc aacgaatttg cttgtcttat tcacatgaag
     7501 acttgctgta tcgaggattg tttatctgcc ttccacgttt tttagctcaa ggaggagaga
     7561 tgccatacat tgaagaaacc tcgatcattg agaccagtta ttcattagat gcttctcgta
     7621 atggcgtgat gaataaagag actctaaaaa aattcatttc ttacttagcg ttgatggggt
     7681 acaacacatt gtatatttac acagaagata cctatgaagt agaagacctt ccatattttg
     7741 gttaccttcg cggagcgtat acaaaagaag aagtgaatga aattgttgaa tttgcttctg
     7801 gttttggcat agaagtagtt ccatcgatcc agacccttgc tcacttgacc caatttttga
     7861 agtggtatgc tcaacaagaa ctgatggatg atcaaaatac tctactgatc ggtgaagaaa
     7921 aggtttatgc ggcaatcgaa aagatgatta ttgcctgcaa agaaatgtat cagacagagc
     7981 gtattcattt gggaatggat gaagcctatc aagcaggtct gggacgatat ttaagcctgt
     8041 atgggtattg tgatcggaca actttaatga ttcaacatat ggaccgtgtg ttatcaattg
     8101 ttgataaata taagatgaaa ccaatgattt ggagtgattt catttataag ctattagatg
     8161 aaaccggtaa agataggtat tacaatcctg attttaaact caaagcatta gcagctaaag
     8221 gattaccagc aggattaacc tatgttcatt gggactatgg ttcagatgaa gtttcctcct
     8281 atgagaaagt aattgataac catttgaaat tttgtgacat tgatcattac gttttagcta
     8341 gtggtgccca tatctggggg agactagccc cgaatcatgg gaaaacagaa gcaattgttt
     8401 ctgcagctgt aaaagcttct aaaaataaag gtgtacgttc tgtgatgtta actacttggg
     8461 gagatgaagg gcaggaaaca gagcattggc attccttaat tggggcatta aaattagcag
     8521 attatgtcta ttctgaagaa gagtggaatg attttgagga aatctacgat cgtttattag
     8581 gaacaggtag ttatcaatta atgtccgaat tacgatattt cgatgaagtg gataccatta
     8641 caaataacaa cttacaaatg gcttccgtga gtaaactgct actttggcaa gatccgttag
     8701 ttggtatata tgactttcat gtagccgctc ataacaaaat tgccactaga acaattggta
     8761 cttattatca tgagttagct cagaaattag atatgaataa aaattggaat ccaaaacaac
     8821 caattttaac aactattcgt caacgatata tacttttagc tgaaatttta gcacaaaagg
     8881 cagaactagg catctattta caacactgct gtctggtaga agaccttgca ggattaatgg
     8941 aattagcgaa tcggtgtgaa gcactagcca atcgagtaaa tgaattggca tctattcatg
     9001 aaataatttg gtatcaaaca tacaaaacct atggttggga agtattagaa caacgatatg
     9061 ctgcattagc tcatcgtctg aaaaccgttg cgcgtcgtat taaaaatcat cttcaggggc
     9121 aagatgccct agaggagctt ttggaagaac gattaccgtt ttgtcaatat caagtgccgc
     9181 ttgaaatttc tggctttaat tatcggcaaa gctcggtgtc aggctacaat taacgaagga
     9241 atcttcccgt tgaacggacc acttgctcat ctaatgggaa agtggttcgt tttctattca
     9301 taagtagctt accactaact cttttattaa gaagagaaaa aaagtaggaa gtgagaaaat
     9361 atgttatcgg actattatca aaatctgtca gttataagtg tcaatacaga accattacgt
     9421 aattattata ttccttatgc aagtaaagaa gaagcaaaac aagagttaga aagggaaaaa
     9481 acgagccagt ttatcgatct caatggtaca tgggattttc agtattatcc ttctgtccaa
     9541 cagttaccgg aaaatttttt tcaagaggcc tttactgaaa gctatgatag aatttctgtt
     9601 cctggagttt ggcaaacaga gggatttgac ttacctcagt atatcaatgt atcttatcca
     9661 attccttttg atccgcctta tgtacctaat gatacccctt gtggtgtata tcggcgtcag
     9721 tttcaaattg agcaaacaga tgcacgcttt tacttaaatc tagaaggagc agatagtaat
     9781 tgttttgttt ggataaatag aaactttgta ggctatgacc aagtgagtca tagtacatct
     9841 gaatttgatg tgaccaagta tttacaggtt ggggttaatg aaattcaaat tctagtgatg
     9901 aagtggtgtg atggtaccta ctttgaagat caggataagt ggcgaatgag cggtctattt
     9961 cgtccagtgt atcttcttca acgaccaact atgcatctga caagttatca actacggacg
    10021 ttgactgatc ctaaaaaagg aagcggaaca ttaatggttt cttttcaacg atttactcct
    10081 attgaagtat cgttacctat ttcttacgaa ttattccatc ctgatggacg tctcatagcg
    10141 aaaggggagt gttactcgga acaacaaata aatttggatg ttgaagattg ccttttttgg
    10201 acagcagaaa cgcctaaatt atatacatta cttttaacat gtaatggtga atattttaaa
    10261 gaaaaagttg gctttcgagc actgacgatc aagaatcgta agttattgct gaacggaaca
    10321 cctattaaat tatatggagt caactatcat gacgtgaatc agcaaggcac gccagtgatg
    10381 acccgagagc agttacgtgc agatttgtta ttaatgaaaa aattaaatgt gaatactgta
    10441 cgaacggcac attaccctaa atcaccagat ttttatgaat tggcggatga gattggtctg
    10501 tatgtaatca atgaagcaga tttggagtgt catggtgtgg ttaatctata tgatactgaa
    10561 gcgaattatc aactaattgc taatgatccg acattcgaaa agccgattat ggaccgagtg
    10621 atccgttcta ttcttcctta tcaaaatcga agctctatca ttatgtggtc tatggggaat
    10681 gaatctggtt atggagtaaa ttttgaaaaa gctcaagctt tagcacggaa attggatgat
    10741 tcacggttga ttcataatga acgctcgatt gaaccagtta ttgaccaagt gaatgatttt
    10801 agtaacttgg atgttattag tcaaatgtat cccccactgg aagatgttca tcgtcattgt
    10861 caaagaagtg atactgaaaa accttttttt atgtgtgaat atagccacgc tatgggcaac
    10921 ggtcctggag atcttaaaac gtacttcgat ttgattcaac agtatgattc ctttcttggc
    10981 ggttgtgtct gggagtggac tgatcatgca atcaatagag gaactttaga aacgccatct
    11041 tatttatacg gtggtgattt tggtgaaaaa tatcacgacg gcaatttttg cgtagatggt
    11101 ttattgaccc cagatcggca attaacagta ggtgcattgg agtaccgtaa tattcattcg
    11161 cctattcaac tgattgctgt agatgaggag ctgcaagaag taaccttgag caatataact
    11221 gcttttacag ccggttcagc atttaacctt tctcttgaat ggtatataga tggaaaaaaa
    11281 acagtttatg aagtaatgaa tcctttcata cttgagcctg gcaaaagttg ttcattgccc
    11341 atcacagtac ctacaacgat tcatggtgct catacggttg ctgtttcgct atctgtttat
    11401 aaaaatgatg agctttatgg acagcaacag ctctttctta aaaaagaaaa aatgagattg
    11461 ccaagaactg ctcatcataa tgatgcacca caaatttcag taaaaggcac agagctgata
    11521 atcaaaggag aatcctttat ttatatatat caattagcaa aagctgcgtt cacttctgta
    11581 aagatcaatc aaaaggaact tttggaacgt cctactcaat tttcaatttg gcgtgcacca
    11641 acggataatg atcgtaactt agtaaacaaa tggaaagcag ctggatatgc tgaaatgtct
    11701 ttagaaatca gaaaaaaaga ctggaaattc gtagatgatc atctcctgat cgaaacgtct
    11761 tatcgaggag ttattccagg attacagcca atttttacaa tgcacataca gttgaaaata
    11821 aatggtgcag gcgtaatcaa ccaaaaagta tcagtggaga aaaatccgat ttttccagat
    11881 ttccctcgct ttgggatgga ctggccgttg aatgatactt tttcgaaggt ttcttattgt
    11941 ggctatgggc cgtatgaaag ttatcctgat aagcactatg ctgcgatgtt tggtatttat
    12001 gagatggaga ttgaggaatt tgatcagcca tatatcaaac cacaagaata tggtaaccgc
    12061 tggcaatgtg attgggtatc aatcaaaaat catcaaggat ctaccatgac ggtttatgga
    12121 aactctaatt tttctgctct gccttatagt caagctcaat taaccgaaaa gtcacaccat
    12181 tatgaattaa taaaagaaaa tagaacttgg ttcaatttaa gttataaaca aaatggtatc
    12241 ggctcacaca gttgcggtcc ttatttagaa gatcgtttcc gttttatgga agatactttt
    12301 gactggaata tagtgattac tttcaaagga gaaaatagat gaaagaaaca aagatcaatc
    12361 aagtcaaaaa aaaattatct cgttatcaag aagttgttta tcgaaaccat tgcttaaaat
    12421 ggtcaaaaaa taagctgaat gttcgtttta atggattatt ttatcgtttc gaacagttag
    12481 aagaagtctg gaaaccggta acgaatttcc aatcatgtcc tgtacagctc ataaatgatc
    12541 caaaagaaac atacgattta tgtataacac acctttataa ccagcatttg aaaaatgacg
    12601 gattttattt aaaggttact aatgacaatc aacttacgat tgaatcagca aaccttcgag
    12661 ggtttcaata tgctatggaa gccttattag agagtttttt ttcggtagga gatcaattat
    12721 tattaccgga attgattctg aaacatgaac catcagtgaa aattcgagga atcattgaag
    12781 ggttttatgg tatcccctgg acacatgaaa tgcgcctctc tcttttttca tatatgaaag
    12841 ataaccaatt gaatactttt atgtatgccc ctaaagatga tgaattatta cgtaaaaaat
    12901 ggcgtgaact ctacgatgcg caagaattag acaaatttaa ggagctttta tctgctgctg
    12961 aggctagcaa tatcgatttt tggtacttaa ttagtcctgg aaatgatatt gatattacgt
    13021 gtgaagaaga cattcaagtt cttctgaaaa agttagaaca actgattgag cttggcgtat
    13081 ttcaatttgg tctattaatg gatgatattg actatcaatt aaaaggtgct gctaaacgcc
    13141 gttttcgaga gccagcattc gctcatgcct atttagtgaa tcgagtagag gaatatctta
    13201 gcactcggtt gtccaatcat gaattagtca tttgtcctac agaatatgat aatcgtcatg
    13261 gttctcgcta tttagcaacg ttaagtcaag aaataccaga acaattaccg ttcttttgga
    13321 ctggcccaag cacgttagcg gcgtcaattt ctacagaaga gctgcaggaa atggcttctg
    13381 tctatcaacg gccgatgctg atttgggata atattccggt aaatgattat ttagaggata
    13441 aagaactatt gttcatgagt ccatatgaga atcgaacacc gaatctttct aaggagagat
    13501 accaggttac aggagtagtt tcgaatccaa tggctcaatt agaagcttca aagtttacga
    13561 tcaatagtat ggctaattac ttatggaact gtgagcgttt tgatcctttg gaaacttgga
    13621 cgtcagtcgt tgaaaaagta gttggaagta aactacaacc agcttatctt accttaacca
    13681 atgcttttcc aaatcattac acaagcagtc tgctttcaga tgaagaatta ctgctagcca
    13741 aagacccaga atgggtcaca aaagaaatgg ctgctttagt ccaagcagta aaatggatcc
    13801 agcaagaaca acatacatct agattgagaa cagaattgga gccttggtta caagcaatca
    13861 cagaaagttg gactttctgg caagaatggc aattaaaaaa agatccaaaa gaagcagaag
    13921 aatggttggc taagaaaaaa acacaccgca taggacgtga tttagtaact gcccatttag
    13981 agcaaattat caaaagttag atatcttaaa aaataaaaag caagaaaaag gagaacaaaa
    14041 tggtcaagag attaattagt gcaaactaca gtgatgtcaa gaaaatgacg tcaaaagagt
    14101 tgaagcaatc catcaaagcg agtgaaggac gggtgattgt ttgtgaaacg gttgctaata
    14161 ctcttccaca atctggagaa attacgaatg cagaagttgc gtcagcattt ggtgcagatt
    14221 tgattctttt aaatggtttc gattgttatc gtcctgcagt ttttggacta ccaaatgtga
    14281 cgattgagaa ggtttttgat ctacagcaaa cgattgaaaa cccgattcct aaattaaaag
    14341 aactagtcgg tcgtcctgtg ggaattaatt tagaacccat tgcccctaaa acaaatatga
    14401 tgagtgagaa agtagaaatc agccaaggaa gagcaagctc tgttgaaacg atacaaaaag
    14461 cagaagaact aggagtcgat tttatttgct ttaccggtaa tccaggcact ggagtaacca
    14521 atttagaaat tactaaatcc gtaaaaatgg caaaaaagta tttttcggga ttgattattg
    14581 ctgggaaaat gcatagcgcc ggctcagaag agccagttgt gaataaagag gcgattattt
    14641 cttatgcgga agcaggcgca gatattttac tagttccttc agtagggtct atcccaggct
    14701 ttttagagca agacctaatt gaagcggttc ggttggcaaa aaaatatgat ttgttaacga
    14761 tgtctgcaat cggtactagt caggaaagtg cccgaccaga aacattgcgg caattagcca
    14821 ttatgaataa aagttgtggt gttgatttac aacatattgg agatgctgga tacgggggat
    14881 tggcaccagc agaaaatatt ttcgaaacct cggttgcaat tagaggaatg cgacatacca
    14941 tcaactgcgt tagccgctcg atcaatcgct aacaaaatta aataaaaaat ggttaaattg
    15001 attatttaag tgggaacata cttgagatat cagctaactt ctgtcacttt aaaaaaggga
    15061 ctgtgacaga agtgaaaata gacaaaaaat atccgaacaa gagactagga tagcagaaaa
    15121 actatcgttt tgcaacgagg accaactgga ccgtaggagc aattctctta ttgttcggat
    15181 atttcgcttg ttcaaggact tatgtctgta gtgccctttt ttggtaggaa aaatggtagg
    15241 gaaccatatc atttttaatg aaaatcaagc atttcaaaag ttgaaaagta agcgccccat
    15301 agaacagtac ctgtacgtca aataacccga cctatagaat agaatcggga atgtttggcg
    15361 tttttagtta cgagaaaaat gttgttgtac atccttgttc caaggcaaca gttgatctaa
    15421 agtttctgct gaaagtacgg gaagatttgg tatcttatca aatagaaact ctagatactt
    15481 tacaggattc aatccgttaa gttctgctgt tttatataca ctcagaatga ttcctgaagt
    15541 tcgtgctcct tcaagacttg tggaatgcaa ccaatttttc cgtcctatca ccagctcttt
    15601 gatatttcgc tccgcacgat tatttgtgag ttctaaacga ccatcttcaa aaataggttc
    15661 aaaaccactt cgtagtttac acgcatattg aacggctgca tccaacttgg attttgcaac
    15721 aggatctatc gttgccatcc actcataaaa agcaataaat aatggacgca gttcttgttg
    15781 ccgacagctt aatcgcttct caggagaaaa gtctttccat ttcttttcta ggacaaacca
    15841 tttatccaat tgtttgactg cttgagctgc gggaatatcc gtgtttttct ttccttttgg
    15901 aattgcttcg aaaaatttcc gccgaacatg cgcgaaacaa tagacaagtc gtacactttc
    15961 cacggcatta tacccgctat agccgtcaca gtgtaaatac cctgtaaact ccttgagaaa
    16021 agttttcgga acatctccag cacgactttc gtcgtggtga taataaacga tgggatgctg
    16081 actgtctttt cctgtacaaa actgccagta ataggtatct gttttggcac tttcaatcac
    16141 acgataactt gtttcatccc cgtgtagaat ctcttcttga ttaagataga ctcttaatcg
    16201 ttctcctaaa gggtctaacg cgttttgcac tgctttaata tgccaattgg tgatattatc
    16261 tctcgaaata tccaacccat gtgtttgttt ccaatagttc tcttgccgat aggcaggaac
    16321 tttctgacca aatttcatcc ggatcgtttc cgccactaaa ctattggaac ctaagctatt
    16381 agaaatcaaa ggtttaggaa cgatcgcttt tttaaaagaa gaaagtcctt ctttttcgca
    16441 gcttttacaa caataggtat gttgataatg ggtgagacaa tccaacatag cctgatggaa
    16501 acgaatttct tctcgtactt ttgtccgtcc gatatctttc atctgtgaac cgcaatgttc
    16561 acaaaattgt tcttccaaag gaaggagatg ttctttctct gttataggta agtgtttgat
    16621 tttgtctgct ttcgttccta caactcgttg atgtcctttt actggaataa tggtggcagc
    16681 tgcttctgga acacttacag aaatttcttc atcactaaaa agattcaatt gcccattcgt
    16741 ttcctcaaag agagtctctt tagagcgtcc aaataatttg ttggttaaat aattgacctt
    16801 ttcattcaat agttggattt cagttgtaag attctgaatg gtttgttgtt gtgcttgatt
    16861 ggttttatgg gcttcttgaa gcaactgaag caacgtatct gtagtttcca aaatttttca
    16921 ccaccttgaa tcgaactgat tttgttacag aataccacaa gaaacgcaca agaaataggg
    16981 gttcaacaca ttttttgtgt taacccccca gcttaaaaaa ctcctttttt tcctggcaaa
    17041 atagctcgag gttgatcgat acttaatcct tctaacaacc atcgaacctg ttggttcgtt
    17101 aaggttcgaa cttcgtcttt cgtccgaggc cattttaact tgccgttttc tatccgctta
    17161 taacagagaa gaaaaccatc accgtcccaa tagagaagtt tgaaacggtc ttgacggttc
    17221 ccgcaaaata aaaaaacggc atcttcgtaa agctcgagtt gatactcttg ttgaataaga
    17281 ctggcaagac catcaattcc ttttcgcaaa tctgtttttc cgcacacaat ataaatatgt
    17341 ttggtttgaa gtaaatcata aagcattgga caacagttct ttcaaaatag cttgaagaat
    17401 ataattgtta ataccgttat agatcaaaac ttcagaacca ttctctcttt tgatgcgcat
    17461 cgctaacttg gcagccgcgg gtttaccttt ttctggtgca ctcttttcgt ttcctaattg
    17521 gacaggaaca atctcattta atttatacat agaaaacctc cagacttctt gatagctcaa
    17581 gtatatcttg aggtgcattt gttagtaagg tactgttcta tggggcgctt acgttgaaaa
    17641 acgttgattt atcggcattc cccatccata ttatatttac gtcttattcc cactcaatat
    17701 gagtagcctc gtaaagaagc tattatatca gtgctttttc agcctattcc tcattttggt
    17761 agggaaatgg tagggttaga tttttactgc ttgatttcca ttaaagtttg caactttaga
    17821 ttttgttgca caggtaaatt taatgtttcc tgtcatttgt tctgcaattc cttcatcatt
    17881 acggctccat agatgagcgt agtgctttaa agtgatttca gggctagaat ggcctaaccg
    17941 ttgcgacacg accagaatgt ctgcgttaaa ctcattgatt aggtagctaa catgagaatg
    18001 acgtaatcct tttccttgta ttaggggtac cttagctagt ttggcataac gctgaataat
    18061 tcgttgaaca gttgaacgat agagtgggag gtcagtgtag ctaagaataa attgttctac
    18121 accatgattt ttttgaataa ctttccactt ttttagcact tcaattgtat catcgtccag
    18181 agaaatgatc cgcaagccgc tttcagtttt tgtgtaaggt ttccgaataa aatctttttg
    18241 atttgtcatt tccaaggtgt gatgaaccct caacttttta cgtttgaagt ccacgtcatt
    18301 ccaattaagt gctaatcctt cgcttacacg aacaccagtc atgtaatata gccagatcat
    18361 taaaaaacac atatgttcgt agaaatcatc tttataaatt tttgccaaaa cttcttcaaa
    18421 ttcttctttt gtccagtaag gaacgattga ttttcctttt ggaattgctt ttgttctttt
    18481 ggaaatattc tcattcagaa attgcagggt gaccgcatag tctaatgatt tacgaaacat
    18541 accgtacata agtgaactgt atgcttgtga gtatccgcta tcgttcaata accaaattcg
    18601 ataattttca caatctcgca cattgatttc acgaagcttc ttatcaccga aaaattcagt
    18661 aatctgaatc aatccacact cacgagaact ccaagtactt ttttgtacgc tagctttgta
    18721 gtgtggaacg taagactgct ccataaattc tttgtaagtt aaatcataat tcgcaaatcc
    18781 atttacaacc atgtattcat tttttatacg catgacttct ttgtgtgctt cacgagctga
    18841 tgagaatcgt tttcctagag aatcttttgt tcctttcttc tggatcctct ttccagtcac
    18901 ttgatctgtt cctaactcaa ctagataaga aaagcgacca tttttatctt gatacacaca
    18961 agggtatttt gttttcatag ttattcctcc tgtgaaagag gaacacctat aatttcttca
    19021 attacttgtc ggggtactac cccaactctt ttattattat aatatggata tccctgattt
    19081 accattcttg tttttgcttg tcggattatt gtggtagctt gatgtgtttt aaatcctaga
    19141 gcctctaaat cttttcgaca aattgtattt ttcattgtat aaactcactt tcaaagaaaa
    19201 cactatcatt gggtaatagt gttatttctt tatttttgta taatgaacgg tactttcttg
    19261 aaaatccatc acttgttcag gacttcccaa ctcatttaaa gctaaagcat tgtaaaaaaa
    19321 tggacgtcta actatatatt cgcttgtata tagtatatct tgattcgtgg aatcataggg
    19381 tttctttacc atctttttgg tgacagttgg tagtttgaga ttccgggact tgaaaaaagc
    19441 ttttttctta tgctctttca attccagctc tttatcgaaa tatttgctga tatagcgccc
    19501 gcgattttcg atactgtcta catcgatctt gttgatctta acgaaaccct gaccccaaat
    19561 cgtagttaac cggttaaatg agataaaggg gaaatcaaac aagataatat ggtagtggat
    19621 tgcaccgcgt tgctgctttt cccacgtggc gagatacttt aaatttgctt ttttagtttt
    19681 atagaggtca taattcagtc gtttgataaa ttttttaaat tcattatttg catactcaat
    19741 gtcttgaatg ttttctttga aagtgagagt taaaaatttc gtttgattgt caaaattcgt
    19801 gtctacaagg cgggcaatct caaatcgcat actttgataa tgtttctgtt tgcgttttaa
    19861 actgtcatat tgtccttgag cggataattc atcatagttt tttctttttt caagagtaat
    19921 atcattttca taaatccaag ctaatttttg ttttcgttcc agatttactt cgttttcgat
    19981 cgttttacgt ttgggagtaa tgattgcttc ataatcataa atttcaatat acgtgggagt
    20041 ttcaatgatt ttctgattat aagcttgcaa aaatcatcac tccttgttgc gtattcgatt
    20101 gtcaaagaac cagtcatttt ctcctgatgt tgatctataa atcaagttaa gggggaaggc
    20161 ctgcgtagca gcccttctag cctatggggt gttgggcggt cttaacggac agaaacggct
    20221 ctgtgggcgc tgccccgccg tttcgtccta accacccaac actccatagt atagttagtt
    20281 ttgtgtagtg gtaccttgtt ttctgggaat caaagttgcc gtatcagcgg ttgccgataa
    20341 cccataactg acaaattgtc cattcgagcg tccccaaggt acaatcacca aattggtaaa
    20401 tgtaattatg gctaaacctt gttgggtaag ttcttccggc gtgatcacag gatgttcaga
    20461 tttgactttg acactgattt tttcgtagcg aagttttggt aggatacatt cgtagctcca
    20521 acctagaatt tcgccctcct tttgccagcg agtgacattg acgacttggt attctttttc
    20581 gagtgtttct tttggtaaac tgagatcggt aatttttatt ggcatcacaa ttctcctttt
    20641 ttatacgaaa gtttatacga tttgattaaa aaatatagat ggccatctac gacttgtatt
    20701 atatacgaga acttatacgt agtcaagaga ttttctaaaa aaatatatac gattttttat
    20761 acgagtgtta taatgaaaaa aatgataagg aggaatttga atatgtttgg tttagtacaa
    20821 cctaataaag tgaaggtggg ccaacgaatc aaagaaataa aagacggaat gtctctctct
    20881 tttacagaat tagggaatcg attaggttta aaaaagccga cgatcagttc ctacgttcaa
    20941 ggctatgcgt tggctcctaa gagtgtgatc aaccaattat ctagtatcag cggaaaacct
    21001 gtcggctggt tttattttgg gcatgtggaa gaatatatcc gagagtattt gtttctcatt
    21061 ggtcagaaaa aaatagtgga cgattatcct gaaatcattg agaaaataaa gcaagaattt
    21121 tatacgggcg agttcaaaaa tcctgcttgg gagaatgagc taggctatcc tttggaggaa
    21181 tttatccaag aatgttttgt agaatattcg agtgaaatca tgaaggacta tgtcgtacag
    21241 attacgaaag aggtacttga agaatccaaa acgctaaaca aactatcatc aaaagcaaaa
    21301 gaagaagctg tcacgattct ttcatctggg atcattgact atatcgagat gagtggtgag
    21361 atcaagtatg gagagaaaga tcgcattgcg aaaatggtga aagaatcaat aaaaaccttt
    21421 gatgtgaaac aagacctgac atttaatgat tcctatttga ttggaaagtt gatcaatatt
    21481 cttggtacag atgaagatac ggagctgttt ctttcttctt tatcaatgat atttactgat
    21541 aagcattttt ccactcgatt tggtggggaa gaattagtcg aaatatttca atcgatgcga
    21601 ccagcgttaa tcaaactcta tcgggagaaa acaccagatg aattttatga ttggtttgag
    21661 aagtaaaata agaaaagtgg cttagattac aagttggtga aaaaattttt ttcagcataa
    21721 catcagtaat ctataaaggg gttgttgatt atactgtaga agttaagtac tcaagagatt
    21781 ttttgtacgt aaacaaagca aaaaagggtg cagtttcact agaaaatgta ctcttttttg
    21841 tgatttttct tagttataga gtttttttga aactttaaaa atgagaaaac atattgattt
    21901 taaagagtgt agacatagct tcttttgcta aatagattaa acttactggt gagtggtctt
    21961 gaacatactt ttaaatcaca ttttactgag ttttattgga ttttataatt cattggaaac
    22021 ctcgtactta atctgttttt atctatcgac tagaatctgt atatattcca gctatcaatg
    22081 aggccgcaga aaagggtgct taaaaataat ctgttgttta aaatattatt atcagaatac
    22141 ttagatacaa aattctggca caaagtaaaa aaaatattga cttaaaaaaa tggatatggt
    22201 aatctataaa cgaaaatagt ggtaacgttt ccaaaatgca tcttcgttaa atttttattt
    22261 ttttaaacga aaatggaaac gtttctaaaa tgtattggga ggagattgaa ttggaaaaga
    22321 cattgggacc aggtggtgag ataagggcgg tacaacggga taaaaaaaat atttcgaagt
    22381 gggaaaaatt aaagaaacag ttgcggcgtg ataagtggat gtacttgttg ctcttaccag
    22441 gtatcatcat gacgtttatc tttaagtatg tgccaatgta cggtgctgtg attgcattta
    22501 aagactataa tcctatgcta ggaattttgg aaagtccttg ggtaggacta gatcacttta
    22561 gggaatttat cagttcacca aattttacgc ttttgctgag caacacatta aaaattagta
    22621 tttatggttt attgttaggt ttttttccac caattatttt agcactgagc ttgaatcaaa
    22681 ttatcaataa aaaaataaaa cagaaaatcc agttgattct atatgcgccg aattttatct
    22741 cagtagttat tattgtgggg atgctgtttt tgttcctatc cgcaaatgga cctattaatg
    22801 ggttagtaga aaaggttacg gggcaaccaa ttatgttcat gtctaatcca gcttatttca
    22861 gaacgattta tattttatcg ggaatttggc aaggaatcgg ctggtcatcc attctttaca
    22921 ccgcaacgct ggcgggagtc agtaccgatt tatatgaggc agcaaatatc gatggggcaa
    22981 atattttaca aaagatttgg catatcgatt tgcctgcatt gaggcctgtg atggtgatca
    23041 gcttcatctt ggccgccggc ggtattatga atgtaggata tgaaaaagcc tacttgatgc
    23101 aaacttcaat gaacatccct gcctcagaaa tcatttccac ttatgtatac aaagtcggct
    23161 tgcaatcagg tgattatgcc tactctgctg ctgttggtct gttcaatatg attattaatg
    23221 tcgttttatt actgattgtc aacaatataa ccaagcggct aaacgatggt caaggcttgt
    23281 aatgaaagga gaaaattatg aatagtgctt tatcaaaaat cgaccgtcaa atcttatggt
    23341 tgaatcgagt gattgtaact tttttagtcg tcatcaccat tgttccgctg atttatgtac
    23401 tggtagcttc ttttatggac ccagctactt tgatcaataa aggtataagc ttcaatccga
    23461 aagattggac aatacaggga tatcaacggg tatttgcaga tgattccatt ttacgcggtt
    23521 ttgtaaattc gttgttttac tcttttagct tcagcttatt gactgtattt attacgatga
    23581 tcactgcgta tcctttgtct cgacctaact tggcggggaa aaagattatt atgacttttt
    23641 ttgtcattac aatgttcgtg ggtggtggct tgatacctac ctatttattg gtaaagaact
    23701 tgggcatgtt agatagtgta tgggcgatta tcgtaccagg tgctataaat gtgtggaata
    23761 ttatcttagc ccgtacctat tttagtgctt tgccaaaaga actttctgaa gctgctacta
    23821 tcgatggcgc caatgacttg caaatactgt tcaaagtcat gcttccatta gcgaaaccaa
    23881 ttatgttcgt attgttttta tatgcctttg ttgggcaatg gaactcatac tttgatgcca
    23941 tgatttacat caaagatccc gacttacagc cgttgcaact cgtattaaga aatattttga
    24001 tccaaaacca accttcacaa gacatggtgg gagccaatac tgccatggca gaaatgaaac
    24061 aaattgctga gttgatcaaa tacgcaacga tcgtcatttc tagtctgcca ttaatcataa
    24121 tgtatccttt cttccaaaaa tattttgata aaggtgtttt agttggttct gttaaagggt
    24181 aagtattgtt cactatttgt atgaataaaa attataaagg agattttaag atgaaagcaa
    24241 aatggatgct gccaatcgtt tttacttccg tactaatttt gggggcttgt ggaggctcaa
    24301 aagaaaagag taaatcaagc cctgattatg aactagaaaa tgtctctttt ccattgaaag
    24361 aaaaagtatc tttaaaaatg atgtctcaaa gtgccccttt ggctcccaat gatcctaatg
    24421 acaaattgat tttccaacga ttagaagaag agaccaatct ccatattgat tggacaaact
    24481 acagtagtga ttttgccgaa aaaagaaatt tggatatcgc tagtggtgat ttaccagatg
    24541 ccatcttcaa cgccgctgca ggtgactatg atttattaaa ctgggcggaa agtggcgtta
    24601 ttgtaccgat tgaagatttg atcgaagatt atatgccaaa tctgaagaaa atttttgatg
    24661 aaaaccccga ataccgacaa atgtctactg cacctgatgg tcacatttac tctatgcctt
    24721 ggattgaaga attaggcgaa ggaaaagagt cgattcatac agtaaatggg atggcttgga
    24781 tcaacaagga gtggttggat aatttgggtc ttgaaatgcc gcaaaccact gatcagttga
    24841 tgactgtgtt ggaggctttc aagacgcaag atccaaatgg taatggtgaa gcagatgaaa
    24901 ttccttttag ctttattaac aatggaggaa acgaagactt aaaattttta atgggggcat
    24961 tcggtctagg tgataatgat gaccacttag tcgttgataa cgatggtcag gtacaattta
    25021 ctgctaacaa agaagggtat aaagaagcca tcaaatattt caatgaaatg aataataaag
    25081 gtcttattga taaagaggct ttcgaacaag attggaatac gtatgttgcc aaagggaaag
    25141 aacataagta tggcgtgtac tttacttggg ataaagcaaa tatcacgggt gcagatgata
    25201 gttatgacgt cttgccagtc ttagcgggac cagatggaac gaaacatgtt actcgtacga
    25261 atggcatggg tttctcaaga gatcgtttcg taattactag tgcgaataaa aacttggaac
    25321 ttacagctag gtggatagat aaaatgtatg aaccgttaca gtctgtccaa aataactggg
    25381 gaacctacgg tgatgaagca caggcgaata tttttgaatt aaaggatggt atgttaaaac
    25441 atttgccttt agaggggaca gcaccggggg aactaagaca aaaaaccgaa gctggaggac
    25501 cattagcagt tcttgattct tattacggca cagtcactac tatgccagat gatgcgaagt
    25561 ggcgtttaga tttgatgcat gacacgtatt tgccatatat tacagaagac aatatctatc
    25621 cacgtgtctt tttagaaaaa gaagaaacag atcgattagc aaaaatcgaa acggatatgt
    25681 gggattacat ctatcgcaaa agagcagagt ggatcaccgg cggtaaaatc gatgaagaat
    25741 gggaagaata ccttgctgaa ttggaacgtg taggtttgga agaatggatt caaataaaac
    25801 aaactggcta tgataatttt ttgaagggag cacaataagt gttagtagaa gaaacggcga
    25861 ttttggataa agcaaatcga tttattgaaa tgatgcgagg aaaagttaac cctcagtatc
    25921 gacaaaacta tcatttttcc gcacccatcg ggtggataaa tgaccccaat gggtttatat
    25981 attacaaagg gcattaccat ctcttctatc aatactatcc ttacgatagt cgatgggggc
    26041 ccatgcattg ggggcatgcg cgcagtagtg atttggtcca ttgggaagat ctaccagtag
    26101 cgttagcccc agatcaacct tatgacagcg gcgggtgttt ctcaggaagt gcaattgaaa
    26161 gagacggaaa actatattta ttttatacag gctgtcaaga aattgatggt attagccatc
    26221 agcatcaatg tttggcggtc tctgaagatg gtattcattt tagcaaatac agtggtaatc
    26281 ctgtggtagc agaaaaacag ctaggtgaaa atgggctaat acaagaattt cgtgatccta
    26341 aagtaattgt tcacaacgat ggttactata tggtagtagc tactaaatct acacagtcta
    26401 ctggtagaat agtgatgttt caatcgtcag atttgtatac ttggcacttt tactctgtgc
    26461 tgctagaagg gacttcagag caaggaataa tgtgggaatg tcctgattta ttccatttag
    26521 acggacgaga tgtcttaatc atgtctccga tacaaatcaa gaagaacgga ctggaatatc
    26581 ataatatcag ttctaccatg gcttgtattg gtgaggtaaa ctgggaaaca ggtcagatga
    26641 tagttgataa tttccatgaa atggattttg gaatggattt ttatgcacca caaacaacaa
    26701 gagatcctaa tggtggacgg gtgatgattg cttggatgca gatgtgggat cgtacattgc
    26761 ctacacatga gttaggccat ctttgggcag gctctatgag tttgccccga gagttaagag
    26821 tgaaaaaaaa tcgattggta caaaaaccca tctcaactgt atatagtcat ttagtttatc
    26881 aacaaggcag tgaaaatatt gcagtgactg acacatcagt cgtatttcga aacgcgctgt
    26941 ctgattcttc atatttacat ctagtcgcag atttggagaa agctgaagtt tttacaattg
    27001 aattactgcg gtctaagacg gattgtctca aactagccta tgacactgct acacaggttt
    27061 ttgaattgga tcgtcagaaa ctaggaagag agcttacagg ggcggaagaa gaccctctga
    27121 atgtacgaaa agttgaagta cctttgattg ataatcagct cgttcttgag atttttagag
    27181 atacctcatc aattgaagtc tttattaatg ggttagaagt tatgacggct actttctatg
    27241 agctggaaaa agctcgagat atcttgtttc attcgatcgg agaaagtcgt attcaagctt
    27301 ttgaaacagc gagtgtgaaa taaatgtttg attgcttagg cgatgaatca attatttaat
    27361 aggttttgaa ggataatgtt tttagaaaag aacagatagc tagatttccc atggatttgc
    27421 tattagtatt ttgaaaaaat tagtaattta tgacaaaatt cattatacta aacttgtgca
    27481 gaaatgtgag ggatcaacta tgaaagcaac aatgaaagat gtcgcaaaat tagcaggtgt
    27541 tggagtagga actgtttccc gggtattgaa taacggttct gttaaagctt ctactagaga
    27601 aaaagttgaa gcagcaatga cagagttaaa ctatcaacca gatttttatg cccgggggtt
    27661 aaagatgaag catacgaaca cggttgcttt gattctaccc acggtatggc atccattttt
    27721 tggcgaattt gcctaccatg tagaaacagc attagaaaga caaaactaca agctttattt
    27781 gtgtaactcc agtggtaata gtgaaaaaga acaagaatat atccagatgg tcagccaaaa
    27841 taaagtagac ggcatcattg gaattactta ttcagatatc gatgaataca tttcgagtaa
    27901 tctgcctttt gttagtattg accgttattt cactgaagag gtgatttatg tgaccgctga
    27961 caatgcggcc ggtggacgtt tggctgcaaa tgaactttat cgccgaggat gtcagaatgt
    28021 agcatatatc ggtgggtacc aagaaacacc gaatgaaacg aagagtcgtc gaaaatattt
    28081 tgaaatcgag tgccgtcaac tggggatttc ctataaagcg ttagatatgt tagaaccttt
    28141 agaccatctc tcagaacgtg taacaaattt tttgttagag aatcccgata tcgatgggat
    28201 ttttacgatc aacgatccaa tggcattagc tgtcattgat atgcttgaaa aaatagggaa
    28261 aaacgtacca gaggatgtcc aagttatcgg atttgatggt caaaagatga cagaaggaga
    28321 tccttatcta gtctcaacga ttgcccaacc tacaaagcaa atggcggaag tggcagtggc
    28381 aaaattaatt gctgtcatta atggagagac agtagaaaaa cgtaccgtat tgccgattga
    28441 atttggcgaa ggtgacacaa cgaaaccttt agtgagaaac gattttgctt agtgcaaata
    28501 cgtttctttt tttgaaaatg agtgaatgaa aggtgtagat aaaatgttat atggtggaat
    28561 agaagccggt ggaacaaagt ttgtctgtgg gattggtaat gaacaattac aagtgataga
    28621 acggaaaagt tttcctacca caacaccaga agaaacgatg gagcaagtgt ttgatttctt
    28681 tgataagtac aaaggaaata tcgcatcaat tggtgtagga gcatttgggc ctattgatat
    28741 tcaaatcgaa tcaagaactt acggatatat caccaacaca ccgaagttag cttggcaaaa
    28801 ttatcatttt gtaggagctt tggaaaacgc cttgtcggtt cccatctatt ggactaccga
    28861 tgtaaatgct gcttgttatg gagaatatat ggcagggtat ggaaaaggta aaaatagtgt
    28921 tctgtattac accgttggta caggtgttgg tggtggtgct gtagtggatg ggaaaatagt
    28981 cgcaggtttt agtcatcctg aaatgggaca tatgctctta caacgtcata atgaagatca
    29041 atttgaagga atctgtcctt ttcatcatga ttgtttggaa gggttagcat caggacctgc
    29101 gattgaaaaa cgctttggaa gaaaagccca gactatctca tttgatcatc cttattggac
    29161 gattgaggca gattatcttg ctcagtgtgt gtataacaca acgttactat atgcaccgga
    29221 agtaataatt ttaggcgggg gtgtaatgaa acagccgcaa ctactcgagt tgattcgcca
    29281 atcgtttaaa gaaaaaatgg gttcctacgt ggatacgcct actctcgaag aatatctact
    29341 aactccaagg cttgctgaca atgcgggctt gattggctgc ctagctttag caataaagaa
    29401 actcaatcat tagaatagta tttattagaa ctattcataa cataccggaa gtttaaaagg
    29461 gtattctgaa acttaaatag tctcaattca ttcttctttc ttgagaatct gagtagagac
    29521 ttttattctg tttcaaatta atataattac gaaatgatat tgagtacaag aaagttcttt
    29581 ttaccagatc ttttaaatcg aaaaatctga tatatatatt tatagtacta aaaatctgat
    29641 tagataaaaa gctaattcaa taggaaaata aaggtagtga ataaaagcaa agcccttgtt
    29701 agtagttaat ctaacaaggg ctttggtagg gaaaatggta ggaaaccatc ttatttttta
    29761 cgaaaatcag gaactttagt aattgaataa tgttgattta taggtattcc tcatccatat
    29821 tatgttggct ctttattccc actccactgt tgcaggtggt ttgctggtaa tatcatacac
    29881 gatacggtta acgtgtgcga cttcatttac gatacggaca ctgatttttt gcaagacttc
    29941 ccaagggata cgggcaaagt cagcggtcat gccgtcaata gaagtgacag cacggatacc
    30001 tacagtgtag tcatacgtac ggccatctcc cataacccca acagagcgaa tgcctggtag
    30061 gacggtgaag tattgccaga tgtcgcgatc taaaccggca ttggcgattt cttcgcgcaa
    30121 gatagcgtct gattcacgga cgatttctaa tttttcttct gtcaattcac caaggacacg
    30181 aatacctaag cctggtccag ggaatggttg gcgccaaacg atgctgtcag gcatgcctaa
    30241 ttgggtacct aattcacgta cttcgtcttt gaacaatgtg ttcaatggct cgatcagctt
    30301 gaattgcatg tcttcaggta atccacctac attgtgatgt gatttgatgg tttgggcagt
    30361 ttcagttcct gattcgatca catcggtata aagggttcct tgtgcaagga aagaaacgcc
    30421 ttcttcaccg gcaagtttgg ttgcttcatc atcgaagaca tatacaaatt catttccaat
    30481 gattttacgt ttttcttctg gatcagacac gccagcaagt tttgataaga aacgttcttt
    30541 ggcatcgact tttataatgt tcaagccaaa ttttccgcca agactttcca taacttgttc
    30601 tgcttcacct ttacgcaaca atacgtgatc aacgaagata catgttaatt gatcgccgat
    30661 cgctttttgt aaaagaacac caacaacaga ggaatctact ccgcctgaaa ggcctaataa
    30721 aacttttttg tcaccgactt gttcacgaat ttttgcgatc tccatgtcga tgaagttatc
    30781 cattgtccag tcaccagtac attggcagac atctagtgta aaatggcgca acaagtcatt
    30841 tccatattcg gaatggcgaa cttctgcatg gaattggatt ccgtacattt tgcgttctgc
    30901 attttggatc gatgcgatgg ggcaatcttt gctggtagca acgacatcga aatcagtcgg
    30961 tgcttgggta actaggtcac catgactcat ccaaacggtt tgtttttcag gagtgttttg
    31021 gaaaagggcc gcttctgcag aagtgatttc caattctgct tttccgtatt cacggttttt
    31081 ggctggttta acttttccgc ctaatttata agtaatcaac tgcataccgt aacagattcc
    31141 taaaactggg atccccaaat caaaaatctt ttcatcaata ccaaaggcct tctcatcata
    31201 aacactgttt ggtccgccag aaaggatgat tcctttaggg ttcattgcct ttacttcttc
    31261 tgcggtaatg cggtggctca tcaactctga gaaaacaccg aactcacgaa tacggcgagt
    31321 gatcagttgg ttatactgac taccgtaatc aagtacgata atttttggct cttcgtcaaa
    31381 tcgtgttggt gaagacaaat ttgaataaaa cttagtccga tatgcggcgc gttctgtgcg
    31441 ccgcatattt ttttagtttt caaagatcag accttgttgt aaatagatcc gtgcccgaaa
    31501 gttataaaag ttgcgataac caaacgcaat tcgtttaatc agcttgatct tgttattcag
    31561 cccctcagtg acgccgtttg agtgggacac cttaaaggca tttgaaatac ccgattggta
    31621 gcgatggaag atcgtgaatt tctttttaaa cgactgaggt aacgtagatg aaattgtttt
    31681 aagggtttct tgaaagcctt ccgtacctcg tttaacgaaa taatagcgta ataattgaca
    31741 aaaatgatac gcagttcgca attcttcgtt gtagcttaac agctcatcaa cgatttctgt
    31801 ttgactaatt ggtcgtttaa acagtcgatg a
//
LOCUS       DAYYCQ010000024        31374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000024 DAYYCQ010000000
VERSION     DAYYCQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.024"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            305..1174
                     /locus_tag="ACHAYF_001139"
     CDS             305..1174
                     /locus_tag="ACHAYF_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8578592.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1383..2408
                     /locus_tag="ACHAYF_001140"
     CDS             1383..2408
                     /locus_tag="ACHAYF_001140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8578593.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2447..3397
                     /gene="manA"
                     /locus_tag="ACHAYF_001141"
     CDS             2447..3397
                     /gene="manA"
                     /locus_tag="ACHAYF_001141"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8578594.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3491..5194
                     /locus_tag="ACHAYF_001142"
     CDS             3491..5194
                     /locus_tag="ACHAYF_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8578595.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5196..7229
                     /locus_tag="ACHAYF_001143"
     CDS             5196..7229
                     /locus_tag="ACHAYF_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8578596.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7240..7680
                     /locus_tag="ACHAYF_001144"
     CDS             7240..7680
                     /locus_tag="ACHAYF_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8578597.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7880..9007)
                     /locus_tag="ACHAYF_001145"
     CDS             complement(7880..9007)
                     /locus_tag="ACHAYF_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578598.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGFGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9010..9687)
                     /locus_tag="ACHAYF_001146"
     CDS             complement(9010..9687)
                     /locus_tag="ACHAYF_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8578599.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9951..11717
                     /locus_tag="ACHAYF_001147"
     CDS             9951..11717
                     /locus_tag="ACHAYF_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8578600.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11732..12169
                     /locus_tag="ACHAYF_001148"
     CDS             11732..12169
                     /locus_tag="ACHAYF_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8578601.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12182..13333
                     /locus_tag="ACHAYF_001149"
     CDS             12182..13333
                     /locus_tag="ACHAYF_001149"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8578602.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13395..14093)
                     /locus_tag="ACHAYF_001150"
     CDS             complement(13395..14093)
                     /locus_tag="ACHAYF_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8578603.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14086..15438)
                     /locus_tag="ACHAYF_001151"
     CDS             complement(14086..15438)
                     /locus_tag="ACHAYF_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8578604.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15661..17061)
                     /locus_tag="ACHAYF_001152"
     CDS             complement(15661..17061)
                     /locus_tag="ACHAYF_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8578605.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17222..17959
                     /locus_tag="ACHAYF_001153"
     CDS             17222..17959
                     /locus_tag="ACHAYF_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8578606.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18084..18563
                     /locus_tag="ACHAYF_001154"
     CDS             18084..18563
                     /locus_tag="ACHAYF_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8578607.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18560..19327
                     /locus_tag="ACHAYF_001155"
     CDS             18560..19327
                     /locus_tag="ACHAYF_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8578608.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19557..20126
                     /gene="lepB"
                     /locus_tag="ACHAYF_001156"
     CDS             19557..20126
                     /gene="lepB"
                     /locus_tag="ACHAYF_001156"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8578609.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20230..20832)
                     /locus_tag="ACHAYF_001157"
     CDS             complement(20230..20832)
                     /locus_tag="ACHAYF_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8578610.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20938..21168
                     /locus_tag="ACHAYF_001158"
     CDS             20938..21168
                     /locus_tag="ACHAYF_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578611.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21159..21407
                     /locus_tag="ACHAYF_001159"
     CDS             21159..21407
                     /locus_tag="ACHAYF_001159"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8578612.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21513..22097
                     /locus_tag="ACHAYF_001160"
     CDS             21513..22097
                     /locus_tag="ACHAYF_001160"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8578613.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22100..23173
                     /gene="prfA"
                     /locus_tag="ACHAYF_001161"
     CDS             22100..23173
                     /gene="prfA"
                     /locus_tag="ACHAYF_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8578614.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23166..24005
                     /gene="prmC"
                     /locus_tag="ACHAYF_001162"
     CDS             23166..24005
                     /gene="prmC"
                     /locus_tag="ACHAYF_001162"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8578615.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23998..24177
                     /locus_tag="ACHAYF_001163"
     CDS             23998..24177
                     /locus_tag="ACHAYF_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578616.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24199..25224
                     /locus_tag="ACHAYF_001164"
     CDS             24199..25224
                     /locus_tag="ACHAYF_001164"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8578617.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25415..26659
                     /gene="glyA"
                     /locus_tag="ACHAYF_001165"
     CDS             25415..26659
                     /gene="glyA"
                     /locus_tag="ACHAYF_001165"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8578618.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26812..27759
                     /locus_tag="ACHAYF_001166"
     CDS             26812..27759
                     /locus_tag="ACHAYF_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8578619.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27893..28522
                     /gene="upp"
                     /locus_tag="ACHAYF_001167"
     CDS             27893..28522
                     /gene="upp"
                     /locus_tag="ACHAYF_001167"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8578620.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28661..28903)
                     /locus_tag="ACHAYF_001168"
     CDS             complement(28661..28903)
                     /locus_tag="ACHAYF_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578621.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29024..29096
                     /locus_tag="ACHAYF_001169"
     tRNA            29024..29096
                     /locus_tag="ACHAYF_001169"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29057..29059,aa:Thr,seq:cgt)
     gene            complement(29215..29853)
                     /locus_tag="ACHAYF_001170"
     CDS             complement(29215..29853)
                     /locus_tag="ACHAYF_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8578622.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29976..30608)
                     /locus_tag="ACHAYF_001171"
     CDS             complement(29976..30608)
                     /locus_tag="ACHAYF_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8578623.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30959..31258
                     /locus_tag="ACHAYF_001172"
     CDS             30959..31258
                     /locus_tag="ACHAYF_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8578624.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10702 a         5482 c         6317 g         8873 t
ORIGIN      
        1 tactcgaaac taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca
       61 tctgcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
      121 ttattacttg atgcagaaca gctttttcac aacctctttt tataagagtt tttactcatt
      181 aatttacaaa aaacaagcaa aatgcgaatt ttctcacatt ttttttaaca aaatcaaagt
      241 tattgcttga aagaagaaga atccatcgtt aaaataaagc ttgacgtaga ggggagcaag
      301 aaatatgaaa gattttcaat tgggggctgc cacctattta aaaagtgtag ccattcgggt
      361 gaaagaccga gataaaatga ttgattttta caagaacgtt gttggctttg atttgaaaag
      421 agaagaaaac gaattagcca ttttaggaac agaagaagct ggaagtgaaa tgttgctgct
      481 ggaagaaagt cctagagcga atgatcacat gggggtgatc aaaaagctga accgtttttc
      541 attgattatc cctagcacag aagaaatggc agatatcctt tgtcgtatcc gtaaaatgaa
      601 ttatccaatc aaagaagctt tagaagatca tggacgtatg gggattttgc tcgtagaccc
      661 agaagagaat gaattggaaa tttaccatga agagaataag aacgaccaaa gagaaattcc
      721 agaaacaatg gatcaagaag cattattaag aaaatctgaa gaaaaattca gtaaactatc
      781 aacaggttct tattttgaaa aaatccattt aaatgttact aacttaacaa aagaacgcca
      841 atttcttaat catgtacttg gtttgaaggt acataatgaa gaagagggca tccatgtatt
      901 gaatgaagga gatttccatg ttggtttgac agaagctagc ggaggcgcaa ttgatttgcc
      961 tacagatgaa gtgattggac ttgattttct gaaattcatg atcgatactg aaagcatgga
     1021 aggactgatt gctcacttgc agcaactatc agttgatttc ttcttagaca aaaagcacac
     1081 agtactcaca atctatgatg ctataggaat cgaatggtgg ttcgtcagtg agccaaaaga
     1141 aaagaataaa actgacattc gatgcgacgc ttaacttttt ctttcttaac gattgtcgca
     1201 tgtctcgtca ttttatgaac ggtcatacta tcgcttattc tagagataga aagagaaaat
     1261 agtataccgg gggaaactgc gcggtttttc gcgcagtttt tttgtgtaaa aaaggttgat
     1321 tcctcataga ttaagcttta agcttggact gtaagaatct gatagaaaag caggagaaaa
     1381 agatgtatcc acacacgaaa cagcttattt tgaaaaagat ggaagaagga ttttatccag
     1441 gtgtagcagt cgccttcata aaagaaaatc aagtagaaac atttacagcc ggctttgccc
     1501 aagtcgttcc tgaaaagcag ctgatgacag aagatctgtt gtttgatgca gcctcactga
     1561 cgaaagtcgt tgcaaccact tcattagttt tacgtctatt agaagatgga aagatagaac
     1621 tcgatcaacc gcttcataaa tatcttcctg aatttgaaaa tcagacaatc actttgcgtc
     1681 atttactgac acatacttct gatatcaaaa catggatacc aaacagagat aagctatctc
     1741 aggaagaatt gatcgccgct tatttgaagc tagaaccagg tgagcaacta ggaaaagaag
     1801 taaaatatac agatgccggt acgattcttc ttgggttgat gctagaggaa atttaccaag
     1861 aaactgcagt ggaaatcttt caagaacaag tattggaacc attaggaatg atgaacagct
     1921 tatttcttcc ttttccttcc aaaaaaatcg ttcccacaga acaactgcct tcgggtgagg
     1981 ttttgcgtgg acaaactcat gatccgaaag caagagtttt ggctgaacat gcaggtaacg
     2041 cgggtctctt tattgatttg aaagacagca ttacttttgt ccaaatgtat ttgaacaagg
     2101 gaaaaatccg taatggtcgt tttttagaag aagagacgat tgattcttta ctgcaagatc
     2161 aaacaccgaa aaaaaatggc tatcgttctt taggatggga cttaaaaaag gatgtagaag
     2221 acggtcatgc gattcttttt catactggtt atacaggtac gtttttcttg attgatataa
     2281 aaacacaaga agcgttgata tttttgtcaa atcgcgtaca tcctgtcgat catcgtgcgg
     2341 agtatctcat atgtcgagat gaaattgttg aaaacttttt gaaagagaaa gcggtattac
     2401 cgaaataatg ctataatagc tatgaaaaat gagaggagtt tgaattatgc aggaacctat
     2461 gtttttaaaa ccagtttttc aagaaaaaat ttggggagga agtcgtttac gttcagtctt
     2521 tggttttgat atccctaacg ataaaatcgg agaagattgg gcgatcagtg cgcatcctca
     2581 tggtgtcagt gtagtagaaa atggcgaatt caaagggaaa agattagatg aactatggtc
     2641 agaacacaag gaattgtttg gccatccttc agaaccagtg tttccattat tgatcaagat
     2701 cttagacgca gaagatgatc tatctgtaca agtacatcca gatgatgcct atgggatgaa
     2761 acatgaaggt gaattaggaa aaactgaatg ctggtatatc atcgatgctg aacctggtgc
     2821 agaaatcatc tatggtcatc atgcaaagac aagagaagaa ctagctgaga tgatcaaaga
     2881 tgggcgctgg gatgaccttt tgaagaaagt accagttaaa aaaggtgatt tcttttatgt
     2941 accaagcgga acgatccatg ctattggtaa aggaatcatg attttagaaa cacagcaaag
     3001 ttctgacacg acttaccgcg tgtatgacta cgacagaaaa gatgcgcaag gacaaacaag
     3061 agagctgcat atccaacaat cgatcgatgt aacaactgtc cctgcgaaaa caccagaatt
     3121 acaaatcaaa gaagtccgaa aagggaattc atcgatcgtc acttatttag aaactgagtt
     3181 tttcaatgta tatgagtggg atattaaagg aatcacatct ttcaaaaaac aagcccctta
     3241 tacattgatg acggtaatcg aaggagcagg agaccttgtc gttgacggaa aaacatatcc
     3301 attagaaaaa ggaacaagct gtattattcc aaatggcgtt tctgagtggg cggtccaagg
     3361 tgaactagca atcattgcgt ctgaaccagg caaatagttc ccagatagct gaaagcagca
     3421 gacggttttt cgtttgctgc tttttctaca taaaatccgt ccatcaataa atagaagtct
     3481 ggtgaaaaag atggaataca aatggaaagc aaacaacagc agtcgcgtgt ctatgaaaca
     3541 actgagaaaa gctggacatc acttaaatga aatgatcatg ccaaatctaa gtctgtttat
     3601 cgcttgggga atcttgtcat tagcaacaaa aggtgtgtct ggcgatttgc acaccacatt
     3661 agaagaggta tgccattgga tgatccagct gcttttaccc atcttgatca gttattcagg
     3721 cgggaaatcg gtagggggac aaaaaggggc agtcgccggt gcaattgctt ctttagggtt
     3781 gatcgctcgt tccaatgctc cacaaatcgt aggagcgatg attatcgggc cgcttgccgg
     3841 catcgtatat gaacaatttg tttctagatt ccatgtgaaa ttcaaggctg gatatgaaat
     3901 gcttgtcagc aacctactag taggactgac tggtagcgtt atttgtataa ttggaatcgt
     3961 ttttattgaa ccagttttaa atagtgttca cttgttgctc gcctccgttg tgtcttggct
     4021 agtcacaatg aatctgttac cacttgtcag tctgatctta gaacctctaa aagtactatt
     4081 tttcaataat gcaatcaatc atggtgtatt gacaccttta ggaatcgaat tctcacaaac
     4141 agtcgggtac tcttccttat ttttgatgga ggcaaatcct ggacctggtt tagggatctt
     4201 actttctatc ctttgttttg cagaaaaaca ggaaaaagta aatgcgagtg gagcattgat
     4261 gatccatgcg attggcggga ttcacgaaat ctattttcca tttgtcttgc tgcgaccgta
     4321 tctattttta gctgtaatgg ctggtggggc aagcggcacg ttgatatttc aatggttaga
     4381 tacgggatta gctgcacctg tatcgcctgg gtcactactc gtgatttttg ccaatgcgcc
     4441 ttatccacaa ctagctgggg taagtatagg gatagcaatc agtacatttg tgaccttcat
     4501 ttgtgcagcg atactattga aattaccaaa taaagaaaaa gagaacaaaa cggtcaaaga
     4561 aaggggagaa atcttggaac catctatcat tcattccgtc atttttgctt gcgactcagg
     4621 tatgggatca agtgctatgg gagccgccct gctgaaaaag aaattagcag cagaaaaaat
     4681 cgatctttct gtcggataca gctctatcta tgaattgaat aaccaagtaa atcagctggt
     4741 tatcgtgcaa catgaactag cgggtcttgc aagagaaaaa gtacctcaaa gtcaaatcta
     4801 tgtactggat cactttttgg aaacagatat cttgctgtca cgaataaaag aagcgatcca
     4861 atttccaaag cgggaagaaa aaaaagagat agcagatatc acagaacaaa aaacgtcgcc
     4921 tgctaatatt gtatttttgt ataaaaacaa cagacgcggc tcacaaacta tgggcatgac
     4981 gattttaagt caattagcaa aaaaagcgaa aaaacagttt gttatctcaa aagaatcaat
     5041 agaaaaaatc aaaatccaag aagaaacgat ttatatcgca caaagagaac tagctgtaac
     5101 tgaacagcta gaagaaaaga tccctcggct agttctagtt gaacattggg tagctacgaa
     5161 tgaatacgaa aaactcgtga agggagaaat ataagatgta tttatcgccc agagaaaaac
     5221 aattattagc agaatttctc agtagcccgt cacctgtatc tatccaaaaa atgatgaatt
     5281 tattgaaggt cagtaaacgg actatttacc gcgaattaga taatcttgaa gtctcattga
     5341 aatcggtaaa tgcttctttg aaaaaagcag ctagaggctc atttgtcatt gaggcatcag
     5401 aagaagtatt agagcaatta aaagaaaata cagatgggaa aagtaatgac ttatccactg
     5461 ctaaacgaca acacgcgatt ttagccgagt tgttactgac gacagagccg ttgagtcttg
     5521 ctcatttcat ggaatcatat cgcatcagta ataccacttt ttatgcagat atcaaacagt
     5581 tggaagaaag tattcgccaa ctgcctttgg aaatcattcg gaaccaggga tacgagatcg
     5641 aaggaccgga aaaataccgc cggcttctga cagcgaatgt attagaatta gaaatcaatg
     5701 aatatgaact ttttcatagt atttctttcg atgcatcact gaattatttt tttcgattcg
     5761 ttgatccgca acatctatct cttgcacgca aagtaatcgg ggaagaattg atccaaaaaa
     5821 agaccaatct aagtgataga aagcttgaac atttggtttt gatgctgaca ttgacgatgg
     5881 agcgtgtgac gaaaaatcat ttactgacaa atgagacata taatagtctg gcaaacaaag
     5941 aattactaca gacagctaaa cgattgtttt cacggatcgc ttctgagaca aaacaacttt
     6001 atccagtaaa tgagatcgtc ttttttgcag gattattaaa tgacttcacg aactcatttg
     6061 atcagaattt ctttgaagag acgttcgata cacagcttgc ttatttagta aaacaactga
     6121 ttcaagaagt aagtgaagag acgaatgtcc atttttatga agatgaaact ttgtacaaaa
     6181 tgctgttgac tcatttatct ggtgtgtttt caagagccgt tttacaagaa gagacactga
     6241 ccaatccgat tttagaaaaa atcatggttc aatacgagga agttgcgaat gcaatccgta
     6301 aagcagtaga gcggattttt caagaaaaaa aattatccga agaagagatc gcttatatgg
     6361 tccttcattt tgcgaattca ttggaacgaa gtccgaaaga aagcaatatc agtattgcag
     6421 gattttcgcc aagtggatta gcttctacta gcatgttgga actacgattg aaaaggtatt
     6481 ttcccttcat ccaacaaatt catttctatc ggattgctga tttaaaaaaa atcgatttac
     6541 aagaagaata tgatgttgtt atttctacat ctattttacc aggatacggt ggaaaatata
     6601 ttttagtctc cccgctactg ctagaagatg aagtcaagca gctaaaagaa gctttttcgc
     6661 atattgagaa gaaaaaaagg caaaaaatta cgtataacag caataacgag tggatgatag
     6721 aagatactta tgaggaaaca atgcatttca tggatcaaat caatcaatta ctcggtcgtt
     6781 tttttatcca agaactggat aatcctgaga cgatttccga aacgattcgc agcttatttc
     6841 cttattttcc tgaaggacta gtaacggatg aagaggtggt tcatcgccgg ctgcttaagc
     6901 gatatgaaca agcacctatc ggtattcctc atacaaatat ggggttattc catacatcca
     6961 gctcattgat ttctaaaccg atcttttgta ttttcaattt gaaacatccg ttgaccattg
     7021 aagggatgga tagacagcca gtcgatttgt cccgtatgtt agttatgctg gcgccttcgc
     7081 caatccaaga aaaagacggg attcttttag gaaagatcag tggagcaatc atcatgaatg
     7141 acttgaacac cgagatcttc cgctcaggaa ataaagaaat cgtttatcag ttgttagcga
     7201 aaatcctgat tgaagaaata aagaaatgaa ggtgaaaaga tgatacagct accagcagaa
     7261 aaaatattgc tcgatcaatc ctatcaaaat tggaaagaag cgttgaccga gatcggccag
     7321 tggatgaccc aacaaggggg aatcaaaccg gcatatatcc aatcaatgat cgaaagacaa
     7381 gaaaaagcga gtgtctatat cgggaatttc gtagccttgc cacatgggac tgacaaagat
     7441 gaagaagtag tagaagaagg aatctattta gtccaagtgc ctgatggtgt ggactttgga
     7501 acaaaagagg aaccaaaaat cgcgacactc ttatttgtag tggctttaaa aaaagaacat
     7561 cagttagcgg tgctccaaga tttagccttt ttctgttcag acatcgacca agtaatggcg
     7621 ctcagtgatg cggaaaccat tgaagaagtc caaatgattt ttgaacatgg acatatataa
     7681 ccaatgttca cagtaaaaaa agctgccttg catcaagtaa taacttcaac caagataaaa
     7741 ataagaggag ctatttttgt tgggaggttc ttattactgc ggatgcaagc ttttgaaaac
     7801 tatttattct agaaaaccgt ttatttgaaa taggggacgg tgacaaggtt tttgtcaccg
     7861 cccctattat ttttgtgttt taatagctct tttttatata gatagtgccc atacctatga
     7921 ataataaaac gatactccat ataccaagtg tcaagatacc attcatgaaa gtaacctgtt
     7981 ctgaagctag tggcgagaaa atatctcctc ctatgatcac attttgaaaa tcaacataag
     8041 aagttggcaa gtaatgactg attgattcta tccatccaat cgaagaaaac aagcgatact
     8101 ctgctaagaa aagacaaaga agtgtaccta aaagattgac tagagaatgt tcaaataaag
     8161 cagatagaag aaaactgatt gtacttaaaa agagcaccca caacaacagg aacagaaagc
     8221 tctggaaaag atactcatac atcggcagga ttcgtatctc ctgaccaatg atcgttccta
     8281 caggataacg gaagtctccg aaaccatttt tgattattac tagtatagag acgatcaaaa
     8341 atggcatcac gagacttagc atagctgcac cataataaat ggtaaaacgg atgctatgtt
     8401 tctttaccat actatccagt aatagattga ctagagagat tacctgcttt cttttttcat
     8461 aagagatgat ggaagaaaga aacaaggcgg ttatacataa aatcaaaaaa ctagaaatca
     8521 ttcctgaaaa aatattttcg tagtaatttg ctaaagagag atgcgctggc aagtcaaaaa
     8581 tagaatatct ttggatctga tgttctttca tataacggag caactcgaca cgtttttgtt
     8641 gttcaatgat tggaattcct tgtctttgac cagaaatcag tcgatccagt atatcttttt
     8701 cataatgata agttgcttcg accatttgct ggacattccc tgtgtttttt gcatgtaaga
     8761 ttgcttcgat cagtttgttg cttttctcta aactttccac aaattctttc tcattctcat
     8821 cttctttcaa cgtttcaatc aaacgttgca tctcttcata gttatgattc agttcatcga
     8881 attctgtata agcaagttta tgatcaaaaa aaagtgggaa tgcaaagagc acgaccaaca
     8941 atagaggaaa taaaaagttt actttgtttt ttagtaagag attgcgttca aatgtaagta
     9001 aagacatagt tacacctcat ataatttacg ataaatagct aaaatctctt ccatatttga
     9061 ggattctgtg acgatttctc cattttttag aaaacatgct ttagaacaaa tcgattggat
     9121 attcgcgata gcgtgggaag aaagaagaat cgtcttcccc tctttctgcc attgtctgat
     9181 gaggctattc aagatatcaa tacttcctgg gtctaatccg ttcaacggtt catcgaataa
     9241 catgacagat gaatcactga tcaagtaagc agcaagcaag acatgttgtt tcattcctag
     9301 agacattttt ccaatcggca aagtaaggta ctcagtcatc ttcaatcgtt ggaaaacagt
     9361 ttggcattct actttgctct gccaatagta tttgatcaaa gtcaaataat caacagccga
     9421 tagatagtcg gataactgat tcgttgattc tagaaaaaag aaagaccggc gataatctgt
     9481 ataagcagta ggagagatgt tgtgaaggga caattgatca taagttgttg gcagcagttg
     9541 ggcaatgcca ttcagcaaag ttgttttgcc aaatccgttc ggggcaatca aagcagctac
     9601 ttctcctgat tctactaaaa gattcaattg tcgaatcaca cgttttttac cacgtttcat
     9661 gttcatgttt ttgatcagta atgtcataga taggcctcca atataaaatt taataacata
     9721 atagaatgga aaacatctaa attaaatgct aacaaaagtt attgtttgca tattataaaa
     9781 aaattaaaat aacagtttta tttaaaattg aagttttctg ataccgaaat ctttttattt
     9841 tgctgacata ctcaaacctt tggcacaaag tttcgtgcca aagtacgact atttctcttt
     9901 ttagtaagcg gatacaatta gagtatcaaa aggagagagg gagagacaaa atggaacgga
     9961 cgattcccaa aaacaacatt tcgctaaaag cgagggtaca aaaattaggc agtactttat
    10021 caagcatggt catgccaaat attggagcac tgattgcctg gggtgtatta acggctctat
    10081 ttatcccaga tggttattta ccaaatgagg ctttcgctac catggtaagc ccaatgctaa
    10141 cttatctgat tcctttattg atcggttata ctggcggaaa agtaattgct ggtgaccgcg
    10201 gatcagttgt tggggcaatt gcaacgatgg gtgtgattgt cggaacagat attccaatga
    10261 tgctaggcgc gatgatcatg ggacctcttg gtggatatgc catcaagaaa tttgatcagc
    10321 tattccaaaa acggatcaaa tccggttttg aaatgctagt caataatttt tcagcaggtc
    10381 ttatcggttt tggattagcg ttattaggtt tttctgcgat tggtccagtt gtcgatgcat
    10441 tgactcaagc aatggcaaaa ggtgtagaga tcattttatc tgcccatttg attccattga
    10501 caagtatttt tattgaacca gcaaaaatct tatttttgaa taatgcaatc aatcatggga
    10561 ttttgacacc attgggaact gaacaagtag cagaggctgg aaaatctatt ctattcttgc
    10621 ttgaagcaaa tcctggtcca ggacttggcg tattacttgc ttttactttg tttggcaaag
    10681 gagcagcaaa atcttcagca ccaggagcca tgatcattca ctttttaggc gggatccatg
    10741 aaatctattt tccttacgtt atgatgaaac cgttattatt cttatcagtt ataggcggag
    10801 gaatgtcagg aagtttcatt ttccaattga tggatgctgg actaagagcg ccagcttctc
    10861 cagggtcgat cattgcgatt ctagcgatga caccgttcaa ttcttacctt cctgttattt
    10921 tgggcgtgat tgttgccgca gctgtttcgt ttggtatttc tgcagtaatt ttgaaagcgg
    10981 atgcgaaaga tacaaatgaa gacttcgaaa aatctgttca agagacaaag gcagctaagc
    11041 aagcttccaa aggtgcccaa ggtattcctc aaggagctac attctctggc gtacaaaaaa
    11101 tcatttttgc ttgtgatgca ggaatgggat caagtgcgat gggcgcttcg atcttgagaa
    11161 aaaaagtaca ggaagcagac ttgcctcaaa cggtgaccaa tcaagcaatc agccaactga
    11221 cagatgactc gaatacattg atcgttactc aggtcgaact acaagaaaga gcaaaacaaa
    11281 aagcaccgag cgcgcgtttt gtctcagtag aaaacttttt gaattcacct cgatatgatg
    11341 aaatcattca agaattaagc agcaagcaga aaacatcagt ttctgaaatt gagaatgagc
    11401 cagcagaaga gtttctttca gcaaacatca aacaaatcaa agagattcta cttgtccacg
    11461 atgatcgttc cggatcagca acaatgggga tgaccgtgct caaagatata ttagacaaga
    11521 accatttaac tatcccgtta agaaaagtca atatccaaga tttaaaagat gaacctgatt
    11581 cactgatcgt agccaaagaa agtttagcgg aaaaagtaca gatggctgca cctcaatcca
    11641 ttcatttatc agtcaacagc atggtcaatc cgcaaaaata cgaatcgatt gcacaatatt
    11701 tgaaacagac agcttaaggg aggaaaacag catgcaaagt ttgccattcg acatgatcga
    11761 gctcaatcga tcttttaaaa ccaaagaaga agcgatccgc tattgcggtc agatgcttgt
    11821 agaagcaggg tgcgttgatg aggaatatat cgatgccatg gtagatagag acaaaatgtt
    11881 atctgtttat atggggaatt ttatcgcgat tcctcatgga acagatgaag caaaaaaata
    11941 tgtgaaaaaa tccggcatct gtgtcgttca agtaccagat ggtgtagatt ttggtaccga
    12001 agaagaagaa aagattgcga ctgttttgtt cggtattgca ggagtcggag atgagcattt
    12061 gcaacttgta cagcaaatcg cactttattg cagtgatatg gacaatgttg tccagctagc
    12121 agacgcgctc agcaaagaag aagtaacgga aaatctagca attgcttaag gagagagaaa
    12181 aatgagagca gtacattttg gtgcaggaaa tatcggtcga ggatttattg gagaggtttt
    12241 ggcagctaat ggatttgaga ttactttcgt agatgtgaat cacacgatca ttgatgcttt
    12301 aaatgaaaga aaagaatacg aaatcgaatt agcagatgaa tcaaaagaaa aaatcactgt
    12361 aaaaaatgtc aaaggaatca acaatcaaac aaatccagaa aaagtaatcc aagaaatcgc
    12421 tgaagcagat ttagttacta ccgccattgg cccgaatatc ttacctttta ttgctgagtt
    12481 gattgcaaaa ggtatccaag aaagagaaaa ggaaggaagc acaacatctt tagatatcat
    12541 tgcctgtgaa aatatgattg gcggttcagc atttctagaa aaagaagtgt ataaatatct
    12601 tccggaaaca tcatttactg acaaatatat cggctttcca aatgcagcag tcgatcgaat
    12661 cgtcccatta caacaccatg aagatccttt attcgtccaa gtggaacctt tcaaagaatg
    12721 ggtcattgac gatagccaaa gaaaaaataa aaaaatccaa ttaaaagggg ttttatatgt
    12781 agatgatcta gaaccatata tcgaacgcaa actatttagt gtcaatactg gtcatgcgac
    12841 tgtcgcttat acaggtgcct tgttaggtta ccagaccatt gatgaagcaa tgcaagatgc
    12901 gttagttgtg gcacaattaa aatcagtttt gcaggaaaca ggaagtttgc tgattgccaa
    12961 atgggggttt gatgcagagc agcatcatgc gtatattgaa aaaatcattc atcgcttcca
    13021 aaacaaaaat atctctgatg cgatcacacg tgtcgctcgg acaccattac gtaagttagg
    13081 ttatcaagaa agatttacac gtccagtgag agaactacaa gaacataact tgacttgccc
    13141 acatttgacg gcaacaatgg ggattatttt taattactat gatcctgaag atgaacaaag
    13201 ccgtcaattg catgaaatga agatccatga aaacttggaa caattgattc aagaagtcac
    13261 aggaatcaat gatccgaaaa caatcgggaa tatcaagcaa aacgtgaacc gttatgccaa
    13321 gcaagtcgct taaaatagga tacgaaaaag gggaatgtga cgaaattttt gtcacattcc
    13381 cctttttcga ataactaggc tgtttgtcct tcaagatagg gcagatccaa ttctttaccg
    13441 taacgttgtt ctaaagctag gcggatcaaa cgagctgcca ggttctcatt tcttgcaagg
    13501 ataggtccat ggaaataaga accgaagaca tttcggtaaa tgacaccttc agattggtct
    13561 tcaccattat ttccttgacc ttgtaccact ttccccaaag gtcgttcacc ttcacctaag
    13621 aaagtccgac cattgtggtt ttcaaatcca taataagttt cattgaattc ttcgttatgg
    13681 ataacgatat caccgataaa ccggttattt tcctgactta acgtgtagtg atccagtgca
    13741 ctgattcctt ggatcttttc tccattagct cccatgtagt agtgacctag caattggtag
    13801 ccaccacaaa tggctaacat gacgccatca ttttcgatat agttgatcaa agaatctttc
    13861 tttttctgga tgtcttttga aatgattaat tgctcaaagt cttgaccacc gccaaagaaa
    13921 accaagtcat atttatcagc atcaaaaggt tcatgtatac taacgatctc cgtatggcaa
    13981 gaaaccccca ttttttctgc tatatatttc aacatcaaaa ggtttccgtt atcaccatat
    14041 gtgttcatca aatttccata aaggtgggcg atattgagtt cataattagc cacggttcat
    14101 tcctcctttg ataaatcctt gagcagctag ttctttacga agctgcaata cagcagtgta
    14161 agtagctaaa atatagacat gctcagttgg caattctttg attttttcga ttacttctgg
    14221 taattctttt gtttctgtca atttattttc tggaatcccg gcaactttta gacgtaaagt
    14281 catatctgta tgacggtctc ctccagaaat aacagctggg atatccatat cggcaaagtt
    14341 ttcaaaatta ccatcccaaa tccagctgac atcgattccg tctgcgtaat tggcatttaa
    14401 taaagccact aaagaaaatg gataaggagc tagaccaatc atatcgatta cttgattgat
    14461 acctacagga tttttgacaa gtactaatgt acattttttc ccattgatat tgatgacttc
    14521 ttgtcggccg aatactttct cgtcatatcc taaaccttgt ttgattttat caggatcgac
    14581 attgaagtac tctgccactg cagtagctgc taaagcattg tagacattat acataccgcc
    14641 aacttcgatt ccgtattcat gaccatcgat cacgaaatct gctgacacat tgtccatccg
    14701 taccatatca gttaatcgat aatccaattc aggacgtttg aagtcacaat ttggacagta
    14761 atatttccct aagtttgcat aagtgatcat tttataatgc aagatgtgat gacaatttgg
    14821 acataagaca ccgtctgtat tgtaatgagc catttgttca cgatcttctt catggtcaaa
    14881 gccataatat tttctaggat tgactgtttc gatagaattg aagattggtg aatcaccgtt
    14941 actgataatc gttgcatttg gtgactttgc ggcaccgtct acaatcattt tataggtcgt
    15001 atagatttct ccataccgat ccatttgatc acggaaaata tttgtaaata agaacagttc
    15061 tggtttcata tattctgtga ctttgctcaa acttgcttca tctatttcta ggacagcaaa
    15121 ctttttctgt cctttttttg gttttgcctg caagaaagtc gatacaattc cctgtaccat
    15181 attcgcacca gtagggttgg ttagaacttc atcgaattgc tggcgcaaaa tattgactgt
    15241 taaagcggta gtcaatgttt taccgtttgt tccggtgaca acgacgactt ggtagtcttt
    15301 tgccaaacta tctagtatat gtggatcgac tttcagcgct agtttacctg gtaaacttga
    15361 tccgccttta aaaaaagttt ttaatgccca ttgcgacgtt tttccaactg caatcgcaaa
    15421 atgactgcgt attcccatta tgtaacctcc aaaaacgatt tgctgacgaa taaattcaac
    15481 taatcatacc atatgttgaa aaaaaggtta aattgctttt cttattctta agaaaaggtt
    15541 acggctgaaa gtgcatgaaa tcggacaaaa tcgcatgatt taacaaaaaa acagcttgaa
    15601 acaaaatgat tgtttcaagc tgctgtgatt ttattattcc ctcttaccta tattcccatt
    15661 ttaaccgata ataattttct cgcttggata ttcatagcct agatctcgtg tttcttttgg
    15721 cacgaataac atcaatgtat atagcattcc gatccgccca ataaacatca gtgtagcaat
    15781 cgtgattttg cccacaacag acaagtctcc ggtaatccct aaagataaac cagttgttcc
    15841 aaacgcagaa gttacttcga tgatgatcga gatcagcgtt tgttcttctg tagcagataa
    15901 aaaaacgata caaaagaaac acataccaag agaaagcata aagacaacga ctgattttcg
    15961 cacatcatct tgatcgattc tccgtccaaa aatattgatg ttatcttcac ttttgaggaa
    16021 ggaatataga tacaaaccga tgattgctac agtagttgtt cgaatcccgc cgcctacaga
    16081 actaggactg catccgataa acatcagtaa agaaaaaatg atcagtgttg tgatttgaaa
    16141 atcacccaaa tcgtgaatct gcagaccagc attacgagtc gtaatcgaat aaaacatcga
    16201 attgatccat ttgacactca tattggaatc ttggaataaa tggtcctttt ccaatagata
    16261 aatcaagaca gttcccgaaa caaacaagat aacaaaagct aacacagcta atttggtaaa
    16321 caaggaaaaa cgaaacggaa gcttcgcatt ggaacgtttg tataatagcc attcccggca
    16381 ctccatcaat acaggaaaac caattcctcc gacaaaaatc aaaaacatga tcaggatcaa
    16441 gaaaaaatag tcatgtgcaa agggcttgat ggaatcccca gtgacgtcaa acccagagtt
    16501 cgtgacagcc gatatcgcct gataaaaacc gtaaaaaacg gcatcacgcc aacgatcaaa
    16561 atagcctctg taataaaaat aaatagaaaa gaatgtccca aaaagtaatt gaaaccagat
    16621 taaaatagca aaagtgatcc gaatcagtcg gacgatacca gacaaacgcg gttgattcat
    16681 atccgtcatg atcagctgtc gctgtttcag tgtgatccgt cgtttagaaa aaatgacaaa
    16741 agcagtagaa atcatcatga tccccaaacc accaacttga aataatattt ccagcagtat
    16801 aataccgtta tcattgaaca cagaattgat atcaaaagtc gatagtccag tcacactcac
    16861 tgtactgatt gccatgaaca gcatgtccac aaaagaaaca tggctatctg gttcgcgaaa
    16921 aatcggcata taaaatagaa cgagtgataa gaccgtcatc aatatataat aaaaaacgat
    16981 gatttggatc gatgagaaat gattggcagc aaacttctgt cctcgccgct gcagccttcg
    17041 aaagaataaa tcaacatgca cagtactatc ctcctgacaa tagtcgtttc taccagtata
    17101 caaggggaat taggaatact caaagaaatt atcaaacaga taacaataca gctttcgatt
    17161 aaaatagaaa tggcttataa taaggaaaat cctagaaaaa agaaggagga ggaataacaa
    17221 attgattaat gctcaacaaa tcgtagaaaa aatgaaaaat caaaaaataa attatgatac
    17281 agttctgaaa aaaatgatca tgcaatggga gaaagaagaa gtaaagccta agatattaat
    17341 acatacatgc tgtgctcctt gcagtacgca cacattggaa ttcatgtctc aatatgcaga
    17401 gatcacgtta tttttttcaa attcgaatat tcatcctaaa agtgagtatt tgcgaagatt
    17461 acatgaacaa aaacgatttg tagaccaatt taatgagaaa actgaccatc atgttgcgct
    17521 aatcgttgat gaataccgtc caaatgaatt caccaaaatg gttttaacaa accatcttga
    17581 aaacgaaaaa gaaggtggtg ctcgctgcag tgcatgcttc aatatgcgat tagatcttgt
    17641 tgcgcaaaaa gcgcaagaac taggatacga ttactttgga agtgccctga ccatctcgcc
    17701 aaagaaaaat gcagctttga tcaatcagat cggaatggat atccaaaaga tttacgggac
    17761 acaatacttg ccaagtgatt tcaagaaaaa caaaggctat gaacgctctt tgcaaatgtg
    17821 caaagagtat gatatttatc gccaatgtta ttgtggttgc gtgttcgctg ccaagcagca
    17881 aggatgcgat ctgaaagaaa tcaatcggga agcaaaagct tacttaaaaa catcaaccgt
    17941 agtttttgag gaaaattaaa gaaatctttc ggaaaaacgt aagaaaaaaa taaagaattt
    18001 atggtagaaa taaggtagac tgtataattt gtccgtagta aagtaaggaa acaagaataa
    18061 atttcaatga aggaagagat aagatgcgaa aagtagcacc tattttgaag ccaatcgctg
    18121 tattaacaat ctcgacgatt gtcgcgacta ctacaggtat tgtgaatagt tccagctacc
    18181 gtgcaccaag ttcaagttct agtactgtag aagaaacaac accaactact tcagtctcga
    18241 catctgaacc aacaacaacg gaaagtttga atgaaaccac tacgacaagt acatgggacg
    18301 atacaacaac tagttcgtcg tatgatgaga catatacaac tgacagctca agcgaaacaa
    18361 gtaatacggt accagatggc actccatcgg attcaaccaa tccaagtaca agtccggata
    18421 caagtgctag ctccgaaacg ataccaagca gtacgactgt agatgataac acgcaaaatg
    18481 atgatgcagc acgtacagca cagcaaaacc aagattcagg aaatacccaa acgtataacc
    18541 agcaagcagg tgattctcaa tgacattttt actagacctt atcaacgggg tgcataaatt
    18601 ccttggctat ttagacatca gtcctaaata tcttaaccgt ggatatacga ttttaagtgt
    18661 gattcccacg ctttatttat tgagaattgt ttatggactt tggcaaaacc aaaactatct
    18721 tcaatttttc ttatatggac ttgcatttct agtgcttgtt tattttactg ttttaaatgt
    18781 attttattac tttttagata aaaacacgaa agccgatgtc actcagcttt ttgtgaagta
    18841 tttacctgat gaagcgttca acatccagac agaagaatcg atagataatg gaaattcgat
    18901 cgataaagtg aatacacaag aggtcatggt ttcttatgac gaagattatc aattgaaatt
    18961 agccgagaat atgcggtatt tgattggaaa tggtgagatc aaaacaaatg aactcggtcg
    19021 catggatgga tttctggtcg atagaaatac attgtatcct tattactatg tcaagaaaac
    19081 aggagagaaa gagtatgttc taaatattgg acgaagctac tcggacatgg aaaaagtagg
    19141 gaccattcga cttgactcaa cagaagacac attggaacct gtcggattgt tcatcgtcgg
    19201 cggtgatttt gtcaaagacg ggttacgcta tcatgagcct tatcgtctga aattgattgc
    19261 aaaaaaggca cagtcgcagg aagaaccagt ggtttatagt cgtagccaaa gaagaagaca
    19321 ttcataatgt gatgaaacgc cgtgtatcag tggttgcgat aaaaacgttt attcggaata
    19381 agggggatgt gacacgtctt ttgtcacgtt ccttttttat ataacgagaa agataagacg
    19441 aacacatcac ttattctttc ctttcattta taaacattgt agaataaaga aagtacaacg
    19501 aagtattgtg aaaaaacgta ataaataacg aggaaaatac aagtaggagg taagctgtga
    19561 aagaattttt gaaaaattgg gggattttga tccttgtatt agcggctatt ttattagcta
    19621 gagtgtacgt gtttacacca gttactgtaa acgggcattc gatggaccct acattaagtg
    19681 atggacaacg attgatttca tctaagatct caaactatga acgaatggat attattacta
    19741 caaaggaacc gggggatgaa gaacggatga ttgttaaacg gatcatcggt atgcctggag
    19801 atacagtcaa gatggaaaat gaccagttga ccattaatgg caaaaagtat gatgaacctt
    19861 atctggatga gttcaaaaaa gaattttcag aagataagtt acaaggagaa tatgcttata
    19921 gctcaggatt ccaagcacaa gcagaaagtt catccacctt tacgagtgac tttgaatata
    19981 ctgtgccaaa aggaaagtat cttgttctag gcgataaccg cttgatttca aaagacagcc
    20041 gaatgtttgg gttagtcgac aaagatatga tccaaggaaa agttgttttc cgttattggc
    20101 ctctttcaga aattaaaatc atgtgatgaa aaaataaaaa gaagctgtga catgtttttt
    20161 gtcacagctt ctttttccga aaattccttc ttatttcttg gagttgctca ctttttttgc
    20221 aatctcattt taagcttttt ggctgatgga taagaaaagc gaagcgtttt tgttcataaa
    20281 tagcttttcc ccacgatgga aggtatgact agtagcagag acaaagcccg ttttttccaa
    20341 tcgattattc aattcttctt ggctaaagcc attatggact ttcggatgac taatttgttc
    20401 attcttgtca aagtctacga tgatcaattg accgccagga gctaaaattt cataaagttt
    20461 tgtaagaata ttctccgtat caggaatgtg taaaagtaca agtgatagaa ggatgagatt
    20521 agcttggaca gcaggaaatt cgacagaagc atcagcgtgg atcgttcgga catttttcag
    20581 atctgctgct tggatctttt cttctgccat tttcagcata gtttctgaag catcggcaat
    20641 gatcagttct ttgaatcttt ccgcaagcgg taaactaaca agcccagttc cgcctccgta
    20701 atccagcagg actttttggt ggctgtcgcg cggtagataa gtagttagtt cttcgcgaat
    20761 aatcttggct aatgcttgac ggtcagggga atcataatgg ttagcgatgg aatcaaaaat
    20821 attttttgtc atcaaatagc tccttacgta aaactttgaa attttaactt attatagcac
    20881 gtgaaaagaa agaaagcatg ctatactcaa ttcaaaaagg gagaatctag aaagaatatg
    20941 ccgatatcgt ttgtaaaaga tagagaggaa aaaggaaagt gtgtccgaga aatattacta
    21001 gacttgcctg agtggtttgg gcttcctgaa tcaacggaaa aatatatcga ggaatcaagt
    21061 aaactacctt tatggtgtga aaaaagaaaa gaagaatatt taggctttat cactctttca
    21121 cagacaagtg aagatactgc cgaaatctat tctattgtat gggaataaaa aaagactatc
    21181 atcaccaagg attaggaaca aaacttttta aagaagcaga aggttacgca gcaaaacatt
    21241 acaaatattt gcaagtgaag actgtagacg aggggcacta ctctatatat gatcaaacta
    21301 tttgtttcta cgaatcatta ggattttcaa ggttggaagt ttttccaaat ttatgggatg
    21361 aatggaaccc atgtttagtt cttgtcaaaa aattggaaca aaagtgagca atagatttaa
    21421 tactaatctg tgcttgatga aaattctcac tgtaaaaaat gctgaaaatc aagtataata
    21481 ccataggaac aaactggaag gtgaaaaaga gcatggcaca actatttttt aagtatggcg
    21541 caatgaatag cggcaagacg atcgagatcc taaaagtcgc acataattat gaagaacaaa
    21601 ataagccagt tgtcttaatg acaagcggta ttgatacaag agatggttta ggcgttgttt
    21661 caagccgaat cggactgaaa cgtaatgcga tgccgatatt tgaagaaaca gacgtatttg
    21721 acgtaattca gaaaatggaa agcaaaccct attgtgtgct gatcgatgaa gcgcaattct
    21781 taaaaaagaa acatgtcatt gcttttacga gaattgtgga cgaattggat attccggtta
    21841 tggcatttgg tttgaaaaat gattttcgaa acgagttatt tgaaggatca aaatatttat
    21901 tgctttatgc agacaaaatc gaagaaatga aaacaatctg ttggttctgc cacaaaaaag
    21961 caatcatgaa tttacattat attgatggaa accctgttta tgaaggcgat caagtgcaaa
    22021 ttggcggaaa tgaagcttat taccccgttt gtcgaaaaca ttactttcac ccatcaatga
    22081 ttacagaagg agaataagca tgtacgatca attacaagca attgaagacc gttatgaaga
    22141 gcttggagaa cttttaagtg acccagaagt gatttctgat acaaagcgtt ttatgcagct
    22201 ttcaaaagaa gaagcaaaca cgcgcgaaac agtcgaagtt tatcgtcgtt ataaagaagt
    22261 agtacaagga atcaccgata cagaagaact gcttggtgaa aaactggatg atgaaatggc
    22321 tgaactagca aaagaagaat tatctgaatt aaaaaaagaa aaagaagtct tagaagaaaa
    22381 aatcaagatc ttgcttttac caaaagatcc aaatgacgat aaaaatatca tcatggaaat
    22441 ccgtggagca gcaggcggag acgaagcagc attattcgct ggcgatcttt tcaatatgta
    22501 ccaaaagtat gctgaagcac aaggctggaa aacagaagtg atggaagcta acgttaccgg
    22561 aatcggtgga tataaagaag tgatcatgat gatcaccgga gacaacgtat attcgaaatt
    22621 aaaatatgaa agtggtgcgc accgcgtcca gcgtgttcct tcaactgaat cgcaagggcg
    22681 tatccatact tccaccgcaa cagttgtcgt gatgccagaa gctgaagaag tagagatcga
    22741 tattgaagac aaagatatcc gtacagatat ctatcatgct tcaggtgctg gcggacagca
    22801 cgtcaataaa acagcatctg ctgtacgttt gactcactta ccaacaggga tcgttgttgc
    22861 tatgcaagat gaacgttctc aaatcaagaa ccgtgaaaaa gcaatgaaaa tcttgcgtgc
    22921 tcgtgtgtac gataagatcc agcaagaagc acaaagcgag tatgatgcca atcgaaaatc
    22981 agcagtcgga actggggatc gttcagaacg gatccggacg tataatttcc cgcaaaatcg
    23041 tgtgactgat catcgtatcg gattaactat ccaaaaatta gatcagattt tagcagggaa
    23101 attagatgag atcgttgatg cattgatcat gtatgatcaa acatcaaaac tggaagctat
    23161 gcaaaatggg taaaatgacc tatcgagaag tcctgtcacg ggcttcttct tttttagagg
    23221 atcatgggaa agaagcatat agtatccaat ttttattttt ggaacgaaaa caatggaaaa
    23281 aacttgattg gctccttcat atgaacgaag agatttcaga agaagaacag cggttgatcg
    23341 aaaccgatct gcgacttctg cttgcagatc atccacctca gtatttgcta ggatatgcag
    23401 atttttatga tcatcggtta aaggtaactg aagcaacact gatccctcga ccagaaacag
    23461 aggagttagt cgaatggtgt ttagacgaga cgcctggtgt accgttagaa gtgatagata
    23521 ttgggacagg gactggagca attgctatta gtttgaaagc agcaagaaaa aattggcata
    23581 tatctgctgt ggatctttca gaagaagcat tagaagtagc gaaagaaaat gctcagaaag
    23641 aaggaacgaa aatcagtttt tatcatggag atacattagg accagtaatg gatcagcaat
    23701 ttgatgtgat tatttctaat ccgccgtaca tcagtcggaa tgaatgggaa ctgatggatg
    23761 aaagtgtccg gtcattcgaa cctaaaatgg ccctttttgc tgagaacgat ggattagcta
    23821 tttatgaaaa aatagcagtt gaagcaccaa gcgtactaaa atcaaatgga aagatttttt
    23881 tagaaatcgg atttcgccaa ggtgaagcag taaaaaaaat atttcagcaa gcatttccag
    23941 ataaaaaagt ggcgataaaa aaggatcttt ttggcaatga acgaatgatc caagtttatg
    24001 gttgaagttg tggatgaaaa attttttatt tgttttttta ttataaaaaa acacttattt
    24061 gacaaggatg taaacgactt tttggtttat caacaagttt atccactatt gacaaataga
    24121 ttatccacaa acagtggaaa acttgaggat aaactcagac aaaataagaa tgtatgaaaa
    24181 aggggttgtg gataaactgt ggaaacgaaa cgatatgatg aaacagaatt gcaagaggca
    24241 gccgaagcac tgaaagaagg agaacttgtt gcttttccta ccgaaacagt atacggattg
    24301 ggcgccaacg ctcttttacc aaacgctgtc aagaaagtgt tttctgtaaa agggagaccg
    24361 caagataatc cattaatcgt ccatgtcgcc tcttttgaac aagtaaaaga gtatgtggac
    24421 aactttcatc cggcagcaga gaagattgtg aagaacttct ggcctggacc attaactttg
    24481 atatttaaga tcaaaaaaga cactttgccc tcagttgtga cgggaggatt atccacagct
    24541 gcatttcgta taccggataa caaaaaaaca ctagaagtaa tcgaactttc cggtgttccg
    24601 cttgtcggtc caagtgcaaa tacgtcggga aaaccaagtc caacgactgc agatcatgta
    24661 tatcatgatc tacaaggaaa aattaccggg attattgatg atggagctac gcgaattggt
    24721 gtagaatcca ctgttcttga tttaagtgat ccaacagcca tgcctatgat tttacgacca
    24781 ggtgcagtaa cgaaagaaca aatcgaagca gtcatcgaat ctcctgttgc tattgatcag
    24841 catcttgtaa aagaaaatga aacaccaaaa gcccctggga tgaaatacaa acactactca
    24901 cccgatacac gtgttctgat ggtcagagat ggcgattggt caactgcagt tcaatgggca
    24961 aaaaataaga agatccgtgc aggtgtcata gcaagccccg aaattgcgga tcaagtgcgg
    25021 acagatactg cagctgttta tatgtataac gacaactcag tagaagcggc ggctaaagga
    25081 ttgtttgcag gtctaagagg attagatgaa ccaacacttg gattagattt gatttttgta
    25141 caagtgtatc cagaaactgg attggggaat gcttacatga atcgcttgaa aaaagcagca
    25201 ggacaaaatt attttgaaaa atgatcaatt ggttattgtc gcattctttt agtatgatac
    25261 aatgctatca agtaaatctt ctatcagtga acgaaaaact aagcgtaact aatcgtaata
    25321 gaagagatac gaatatttgg caatttgttt ttgatgggaa aaataaaatt gtaggaaaat
    25381 aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa cgtttgaccc
    25441 tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt tggagctgat
    25501 tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta tcttgacaaa
    25561 taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat ttgtcgatat
    25621 cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt
    25681 ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg tagaaccagg
    25741 ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg gatcaccagt
    25801 caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga caacagaagt
    25861 tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat tgatcgtagc
    25921 aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga
    25981 agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag cagcaggact
    26041 tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata aaacattgcg
    26101 tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag
    26161 tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg gtaaagcagt
    26221 agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa tcatcgccaa
    26281 tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca tttcaggggc
    26341 gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc
    26401 agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc cttttgaaac
    26461 actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca ctactcgcgg
    26521 gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa
    26581 agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt
    26641 tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt tcaatgttgg
    26701 gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga tgctctctag
    26761 tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt catgactgat
    26821 cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt
    26881 atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca agaatcacct
    26941 gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga atggggaaaa
    27001 ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt tattttttat
    27061 attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt gatcagagaa
    27121 cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc accagaagca
    27181 aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa taaattagcc
    27241 aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt aggtggtaac
    27301 atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa
    27361 ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca tgaattcgga
    27421 gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata tacgacgaat
    27481 gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa tgaactaaat
    27541 gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga tgaaggtgag
    27601 gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg cttccaagga
    27661 atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac gcgtgcagca
    27721 atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa
    27781 acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag tcaatcactt
    27841 gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa aaatgggcaa
    27901 atttcaagtg atcgatcatc cattgattca acacaaacta actattattc gggacaaaaa
    27961 ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga tggcatatga
    28021 agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca ctgaaacaac
    28081 acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg caggtattgg
    28141 aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata tcggtcttta
    28201 tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag aagacattga
    28261 tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag caatcatggc
    28321 aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc
    28381 acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct atacagcttc
    28441 tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg atgcgggtga
    28501 ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca
    28561 tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc tttcgtttgc
    28621 atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt tttagttctt
    28681 tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt tctgcagttt
    28741 catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca gtttgcgact
    28801 tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca atttccgatg
    28861 gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct gcctttcaca
    28921 tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc catcggactc
    28981 tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct tagctcagtt
    29041 ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag ctggcattaa
    29101 aagagaagac agatatatca aggattttta atccattgat acatctgtct ttttgtttaa
    29161 aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa aaatttagga
    29221 gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg cccaacttgt
    29281 aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca ccccttgtat
    29341 caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac tgtaatggat
    29401 aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact tactagaaaa
    29461 aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag gcaaaataat
    29521 aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc atttcaaaat
    29581 ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa aatatttggc
    29641 tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt ttttaccaaa
    29701 aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa taatggatgg
    29761 cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata tccctttttt
    29821 aagttctatt ttttttccta aaatgttatt catgataata actcctacta ttcagttttt
    29881 gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta tggatatttg
    29941 tattgctaaa aatttatctt atatattcat tttgattata ctacttttat ttgtaaaaat
    30001 tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag atgttccgag
    30061 cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa atcagtatca
    30121 aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag ggaacagtct
    30181 acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat gtttgaacca
    30241 gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat ataactttcc
    30301 atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa catgcttttt
    30361 gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat ctccattctt
    30421 tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat aattggatgg
    30481 cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag ttcaaaatct
    30541 tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt aactttagga
    30601 aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc taaaccaact
    30661 tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt gtcgctatct
    30721 cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc taataaaaat
    30781 cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa aatcgttgcc
    30841 ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat ctagatgtag
    30901 cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg aaatcgtgat
    30961 gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg ttttaaatcc
    31021 agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct tagaaaaagg
    31081 tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt tagcaatcag
    31141 gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa taggaaaatt
    31201 tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc atttataaaa
    31261 acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc aggctaggtt
    31321 catttatttg aacagagtct gtattttttt attctaaata aagaaacaga tgtt
//
LOCUS       DAYYCQ010000025        30629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000025 DAYYCQ010000000
VERSION     DAYYCQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.025"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="ACHAYF_001173"
     CDS             complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="ACHAYF_001173"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8578625.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="ACHAYF_001174"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="ACHAYF_001174"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8578626.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="ACHAYF_001175"
     CDS             8128..9096
                     /locus_tag="ACHAYF_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8578627.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="ACHAYF_001176"
     CDS             9114..10019
                     /locus_tag="ACHAYF_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578628.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="ACHAYF_001177"
     CDS             10016..10822
                     /locus_tag="ACHAYF_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578629.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10979..11947
                     /locus_tag="ACHAYF_001178"
     CDS             10979..11947
                     /locus_tag="ACHAYF_001178"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8578630.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="ACHAYF_001179"
     CDS             complement(12264..12788)
                     /locus_tag="ACHAYF_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8578631.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..14886
                     /gene="scm"
                     /locus_tag="ACHAYF_001180"
     CDS             13075..14886
                     /gene="scm"
                     /locus_tag="ACHAYF_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8578632.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15041..16027)
                     /locus_tag="ACHAYF_001181"
     CDS             complement(15041..16027)
                     /locus_tag="ACHAYF_001181"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8578633.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16050..16616)
                     /locus_tag="ACHAYF_001182"
     CDS             complement(16050..16616)
                     /locus_tag="ACHAYF_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8578634.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16630..17046)
                     /locus_tag="ACHAYF_001183"
     CDS             complement(16630..17046)
                     /locus_tag="ACHAYF_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8578635.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17503..19437)
                     /locus_tag="ACHAYF_001184"
     CDS             complement(17503..19437)
                     /locus_tag="ACHAYF_001184"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8578636.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19739..20674
                     /gene="pplA"
                     /locus_tag="ACHAYF_001185"
     CDS             19739..20674
                     /gene="pplA"
                     /locus_tag="ACHAYF_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8578637.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20764..21867
                     /locus_tag="ACHAYF_001186"
     CDS             20764..21867
                     /locus_tag="ACHAYF_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8578638.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21880..22833
                     /gene="menA"
                     /locus_tag="ACHAYF_001187"
     CDS             21880..22833
                     /gene="menA"
                     /locus_tag="ACHAYF_001187"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8578639.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23647..24993)
                     /gene="gor"
                     /locus_tag="ACHAYF_001188"
     CDS             complement(23647..24993)
                     /gene="gor"
                     /locus_tag="ACHAYF_001188"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8578640.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25127..26380)
                     /locus_tag="ACHAYF_001189"
     CDS             complement(25127..26380)
                     /locus_tag="ACHAYF_001189"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8578641.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26386..27309)
                     /locus_tag="ACHAYF_001190"
     CDS             complement(26386..27309)
                     /locus_tag="ACHAYF_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8578642.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27530..30022)
                     /locus_tag="ACHAYF_001191"
     CDS             complement(27530..30022)
                     /locus_tag="ACHAYF_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8578643.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(30029..30496)
                     /locus_tag="ACHAYF_001192"
     CDS             complement(30029..30496)
                     /locus_tag="ACHAYF_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8578644.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         8862 a         6519 c         5727 g         9521 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttggg aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggtaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    14161 actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact
    14221 acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc
    14281 tctaccacaa gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct
    14341 accacaagtg atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc
    14401 acaagtgata gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca
    14461 agtgatagca cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt
    14521 gatagctcag attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac
    14581 agtacagata catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt
    14641 acagacacgt cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca
    14701 agttcggata acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac
    14761 aaatcaaaaa gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca
    14821 ggcgttatat tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa
    14881 aggtaaaaaa atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt
    14941 tttgcttttt atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt
    15001 ccttctgaga aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa
    15061 tcagttgggt aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct
    15121 ttatttcctt caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc
    15181 ctcctgtatg ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt
    15241 tcttctctaa ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta
    15301 aagaatagac cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt
    15361 ttgtataatc taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat
    15421 ctcctgcttt ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac
    15481 ttaattggaa cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa
    15541 gttcataatt atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac
    15601 tgcgagaatt caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac
    15661 agcagacaaa taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga
    15721 tattaggctg accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg
    15781 ccgtatggag catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg
    15841 gtccaaattg tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga
    15901 tcgtttcttt gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc
    15961 gttttagtgt ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta
    16021 gcttcataaa aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt
    16081 tgtttggttc cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa
    16141 agataattcg ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact
    16201 ggcaagtaca acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg
    16261 acattatgca tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct
    16321 aattgcttga ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg
    16381 aatgtggaaa tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag
    16441 aagctgtctc gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct
    16501 agttttgcac caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg
    16561 atcactcctt gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg
    16621 tgtgaaggtc taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat
    16681 cactaattta tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg
    16741 tacgcagatc tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc
    16801 gatcaagtta taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc
    16861 catcaaaggg aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc
    16921 tgttgcggtg ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa
    16981 aagcaaaata ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag
    17041 gctcattttt gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag
    17101 gttgtgacac ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca
    17161 tgttttctaa ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca
    17221 agaggttgtg acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt
    17281 ttcaagtttt tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt
    17341 ataacaagag gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc
    17401 gggcatctta ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc
    17461 tttctattga aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg
    17521 ggaacttggt ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca
    17581 gcgatccaac ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa
    17641 ccaaatgttt acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt
    17701 tgtcgcactg catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc
    17761 aaagaatacc atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg
    17821 catgatcttt gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta
    17881 tgcataactc aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc
    17941 tccgctggca gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac
    18001 attgtattcc catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa
    18061 ccaagtactt ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa
    18121 ccaaactaaa ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga
    18181 agaatgtcct cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata
    18241 ttggaacata taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt
    18301 cattacgata gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca
    18361 tttggcacca atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc
    18421 gcctttgatc gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg
    18481 aacgatctgc ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat
    18541 atagatgttt ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg
    18601 tccgcgtgct gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt
    18661 ccaaatcagt gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc
    18721 cacttctacg attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc
    18781 agcttttgca gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac
    18841 catcagtgtg atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc
    18901 gatcagttcc ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag
    18961 catggcttta cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat
    19021 atgttcccga atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac
    19081 accaggtgta ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg
    19141 ataggaacct aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc
    19201 tgtgacaagt tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc
    19261 ttctggttct acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga
    19321 atgacgatcg atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt
    19381 tgtagctgaa acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga
    19441 tcatccgctc cttatagact atctatataa tgaatatatg ataattcttt tagtttgata
    19501 cttattacac aatctccata tattatatag atgttctgtt cttttgtcta actccttttg
    19561 gatttttttc aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt
    19621 tgaaatatta catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa
    19681 tgtataatga atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat
    19741 gaagaaaaaa cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg
    19801 cgcttgcggc gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca
    19861 atcttcgact gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt
    19921 gcaagacggc acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt
    19981 tgaaatggtt gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa
    20041 cggcgaatca aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac
    20101 aaacccagaa aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag
    20161 cggtgtggaa gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca
    20221 acaattgatc caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc
    20281 tgatttgaaa gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt
    20341 gcaattcgaa atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga
    20401 caaagacggt aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag
    20461 cggtacagaa ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg
    20521 cgaagaagat ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca
    20581 ttcattcatc atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac
    20641 aatcgaagta gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg
    20701 gagggtggga cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa
    20761 aagatgcgga aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta
    20821 actggctgca gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata
    20881 ttgaaagatc catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg
    20941 tatgatgaag gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt
    21001 gataaaatca cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg
    21061 atcaaaccag taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat
    21121 agccaagatt ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga
    21181 atcggttttg atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta
    21241 gtggattatc ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa
    21301 ggaatgatca tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa
    21361 gtcttgaaaa aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc
    21421 ttaggccaca gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac
    21481 atggctcgcg gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct
    21541 ggaatttacg agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca
    21601 aaaacgggtt atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca
    21661 attgatggtg acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa
    21721 tacgtcgaaa gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa
    21781 gtatatgtga ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg
    21841 atgggtgacc gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta
    21901 gaagtagttg aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta
    21961 ttctctatcg ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga
    22021 atgctagttt ttgatttagc gactaccgct atcaataatt acatggatta tgaaaaggct
    22081 cattctgaag tatataagta tgaagaaaat gtcattggcc gcagaaatct atctcctgta
    22141 ttgattcgaa acatgatttt agctatgatt gcatttgttt ttgttatcgg cgttattcta
    22201 acgatcaata cgggttggct tttacttttg atgggattta tctgttgttt tattggagta
    22261 ttctatacat tcggaccgat tcctttatct cgaatgcctt taggggaaat tttcagtggg
    22321 gtgactatgg gccttggaat ttttgccatg gtcgtttatg tcaatacatt cggttcagat
    22381 gtctatgatc tgacagtcaa cttttcatct ggacaatttg ctttggtagg aaatctttgg
    22441 gctatagcag caatcgtatt agcttcctta cccttggtgt ttacgattgc caatatcatg
    22501 ttggctaata atttgcggga tcttgaccgt gatattgaaa atcatcgcta tacactagtt
    22561 ttttatatcg gacgtcctgc gggaacagtc ttattccagc tattgatgta tgcttgctat
    22621 ttcgtgattt taatggggtt gttgagcggt atctatgaat ggccgatctt ggtcaccttc
    22681 ttgaccttgc caattgtgaa gaagcatttg gatcaattca agcaaacgtt accccagcct
    22741 atcagtttca gctattccat caaaaatatg gtcttattca atgccagtta tgtattgggg
    22801 ctattactaa caatcattat gcaagccatt taacagcatg aagaggttgt gaaaaaactg
    22861 ccctgcatca agtaataaga acagccaaat aaaaatagct ccttatattt ttcgtttggt
    22921 tgggcttatt accgcagatg caagcttttg aacaccgttt atcaggaggt tgtgaaaaag
    22981 tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca tttttgcata
    23041 aatttggctt aataccgaga aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc
    23101 agtgttttgt cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt
    23161 tttttgagct aataccgaag gacagctgat tgaacactat ttgttcggaa aaagtgacta
    23221 actatgacac aatttttttc acagtccctt ttttcacttg atttgattcg taccagcgaa
    23281 cttgacgaga actttcttgg tttggtaact atatggtatt cttttaacat cctcgtttgc
    23341 ttcttctaat ttatttatac aaagtcacac ttttttaaac atctgttctt cgatacattt
    23401 ctatttcttt taaacatcga taccaatatt ttgaacttaa gagtgcatac caaatggatt
    23461 catttactct tcgtcagtta cgaagataat caaaatctcc aacttcccta cttatttcgt
    23521 tttgagaatt atcgaagcaa tcttacataa ataactaaaa aacaaaaaac cagaaaatca
    23581 gtatactgat tttctggttt tacatggttt gttcaataaa aagctgttgg aactgttaat
    23641 attaaattat cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg ctactgtatt
    23701 gtcaaagtct tctttagttg cacccatctt gatagccacg gcaaaacctt gcagcatctc
    23761 atcgacacca acaccaatag catgtaagcc gacgattctt tcttcttttc cgacacagat
    23821 caatttcatt tcgcattttt ggcgataatc atttaatgca aagtacattg gggtaaagcg
    23881 tgaacgataa actttcaatt cattttcgcc atatttttcc aatgcttctt cttctgtcaa
    23941 accaattgtt gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag
    24001 ataagaatct ttctttccat taaataaacg ttctgataaa cgtctaccag ctgcgattgc
    24061 aacaggagtc agatctaatt taccgatcac atcaccaaca gcaaaaatat gttcatccgt
    24121 tgtattttgg tatttatcta ctttgataaa cccttttttg tccaattcca catttgtatt
    24181 ttccagacct aatttttcgg tattcggtac acgtccaccg gcaaaaagta cacaatcacc
    24241 agttaaggtt tcaccatttt caaatagcac cgtatattcg tcattgtttt tagtaatttc
    24301 tttagcaata tgatgtgcgt aagtttttat tccagcttct tggtacattt ggatcaaatt
    24361 gtccgaaagc attttatcaa acgttctaag tggtcgttct tttcgatatg cccacatgac
    24421 ttcactacct aaaccacgaa atacacctga aagttcagcc gcaatgtatc cagcacctaa
    24481 aacgattgct ctcttaggta actctgttaa ttcaaaaaat ccatttgaat caattgcgta
    24541 ttctccacca ggaatggtca ttttacttgc acggcctcca gtagcgatca agattttagg
    24601 agcacgatag gtttcaccgt ttacttcgat cgtttgacta tcgataaaag tagcgtaacc
    24661 ttttatagcc tcgacatgat tgctatctaa tcctctttga taagcaccgt gtagaaaatc
    24721 aatatatttt tctcgtcttt ctactagacc ggcgaaatca acatttttta aatctgcttg
    24781 gataccgtaa cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg cttgccacat
    24841 aactttttta ggtacacaac cgacgttgac acatgttccg cccaattcat tcgcttcaat
    24901 cagcaaaact tttgcgccat gcatccctgc tctgtttgct gaagcaatac cgccagagcc
    24961 gccaccaatt acgatgtaat catatatttg cattttgttc ctccttattg aagagatcaa
    25021 tcaaaacctt tatttaacga tcctttttct acagaaaaca agtgtaacac ttttcttgaa
    25081 ttttgtatgc gaagatgctt aaaacaggga tatatcagct ttttccttat ttacgttttc
    25141 ggatcatatc tccaggtttc accggctgtg cgactggcat agttaaaatc atcattggat
    25201 ttggtgctcg gtcaatggat tcgccttctt cattatgcat aacttcaacg gtttgactga
    25261 aatgactgaa tcctggacca taaaattcga tttcatctcc tacagaaaac aagtttctct
    25321 gacgtatcgt agcaatctgt gttgcctcat cataagataa tacttcaccg acaaatttat
    25381 atactgggat tttacgacgt tcaccgaata attgttcgtt ttcagttggt gtattgtagt
    25441 aaaaacctgt agctaattct cgttgtgcta ctttccataa ctcgtcgatc cattcttgct
    25501 tgcagacata gttttcagga tcttccatat aactgtctac agccgcttta tagacatttg
    25561 acaccgtaga tacataatgg atcgatttca ttcttccttc gatcttgaag ctgtctacgc
    25621 cgttttcaat caattctgga atatgttcga tcattgacat atccactgca ctcatcgaga
    25681 attcttcttc cacttcgcct gtatcagtta aactgtttcg ttcttgacca aaaggcatat
    25741 cgaacaatcc atacttccag cgacaagatt gagaacatcc gccacggttt gcatcccgca
    25801 tcgacatatg gttggacaat gtgcatcgac cagaataaga gatacacatg gcaccatgga
    25861 taaatgcttc gatttcggta tccgtatttt tacgaatctc tgccacttcc tccattgaaa
    25921 cttcacgagc taaaacgaca cgttccaacc cttcttcttt ccagaattcc aatgtctcaa
    25981 aattagttgc tgaagcttga gtggataaat ggatcggcag acctggggct tctgtcgcac
    26041 aaatttcgat caaagctgga tcagaaacaa tcactgcaga aataccgaca tcacgcaaac
    26101 tgcggaaaaa ttcccctgct ccatcttcat ttccttcatg ggtgaccata ttagcagcaa
    26161 cgtatacctt cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt tcttcataag
    26221 taaaattacc tgcacggctt cttaaaccat atgcattccc gccaatatat actgcatctg
    26281 ctccataatg gatcgcggtt tttaactttt ccagtgttcc tgctggcgct aggatttctg
    26341 gtcgtttcaa ttttcttttt actgtcattt ctttcatcat actccttatt tgatagcaga
    26401 aggatcaata taaaagaatc ctgtatctaa ttcacggttt tcaggatgca atttactgat
    26461 ttcgctagtc attttttccg ctttttcttg atcccagcta ccttctaaaa tttgtttttt
    26521 tgcttcggcg aatagtcgag tgatgtttac gaatttttcg cctggagtaa ataatccgtc
    26581 caatttccat gtacagtaat gatgctgata aagttcattc aattcattcg agagattcag
    26641 atcattgctg gcaaaaatat gtgtgccatg actatcttca taaatagaat aatgtgtctc
    26701 ttctttttta ggttccgaga taaaaagacc tctttcacga tctttttgtt cgtcttgttt
    26761 cgtatagtta tagtaattct gtagcagtgg acgtttggat tgatggatgc atgtagcacc
    26821 atacacaagg gtttctacag gaatatcgag tttttcttcc atcgccttca tctcttcaaa
    26881 aggaacttca cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga aattgatttg
    26941 acgtgcgctc gttactaatg tttcagcatc ataaatatac ggtatacgca attcaggatt
    27001 ttttttcatc gtataaataa tacctgtatc accaactgtg atcacatcta cgtttatttc
    27061 ttttaaaaaa gccaaatatt caggtacatt tttcattttt tcaggatgaa tgatcccatt
    27121 tacagcaata ttcgcttttt taccatactt gtgtgctaat tctaccaatt gtcgctgttc
    27181 ttctcgtgta aatgaagcag gcagacgcaa tccaaattgc tcttcaccga aatatagtgt
    27241 atctgcacct gcagcaagca actgttctgc ttgttcaata gtttctactg tggttatgag
    27301 ttcgatcata attttctcct cctacaactt cataatagta gcatagctct aaaaactttc
    27361 caatacttct tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa
    27421 agttcgatat attctgtcca aatataaaaa gagattgtga caaaaatctt gtcacaacct
    27481 cttaatcaag tcttactatt ataaaaattc agctacacag gccaagtaac tatagggaac
    27541 ctactgtttc ctttttaggt ccgcgaacat tcaatgtgat cttacctttt gaagcaccta
    27601 ctgttacgtg gtcacctaaa cgaatttctc cgccaagcaa agcctcgctc aatcgatctt
    27661 caatttcttt ttgaagtgcg cgacggattg gacgtgcacc atattcagga tcaaatccag
    27721 ctttcccgat cacatcgatt gcagatggtg tgattttcac ttgaatatct tgttctttca
    27781 atcgtttgat aatcgctttg ctcatgattt ggacgattgt atggatttca tctttactca
    27841 atgaatggaa gacgacagtc tcatcgattc gattcaagaa ttcaggtcgg aaagctttct
    27901 ttaactcttc taagatacgt ttttgcatgg ctttgtgatc tttagtaaca tcttggacat
    27961 tgaatccgac atttttttct tctcgaattt gtgtcgctcc gatatttgaa gtcataatca
    28021 tgattgtatt tctaaagtcc acttttctac ctttagaatc tgttaaatga ccatcatcca
    28081 atacttgcag aagtaaattg aaaacgtctg gatgtgcttt ctccacttca tctaagagaa
    28141 tcactgaata tggtttttga cgaatttttt ctgtcaactg tccaccttca tcatacccta
    28201 cgtatcctgg aggagacccg attaatcggc ttgtactgta tttttccata aattctgaca
    28261 tatcaacacg gatcagggca tcttcacttc caaacatgac ttctgaaagt gctttggcca
    28321 attctgtttt tccgacacca gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag
    28381 gatctttcaa tccgcttcgt gcacggcgga tcgcacgtga aactgctttg acagcctcat
    28441 cttgaccaac gacacgttca tgcagaagtc cttccagttc taacaagcgt tcgctttctt
    28501 ttttctccaa ctgctgtaga gggacaccag tccactccga tacaactgta gcaacatctt
    28561 cttctgttac acgatcagaa taaccttgct cctgttttgc ttcaattgca gaaacttttt
    28621 gcagcttttt acgtagtgct ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt
    28681 gtttttggat cgctgcttcc ttttcttgta ctagttcatc gatttctagt ttgatcacag
    28741 ttgattttgt taagtgatcg gtttgatcca aacggacttt cgctgctgat tcatcgatca
    28801 aatcaattgc tttatcgggt agttgacgag aagtgatata acgaacagaa agattgactg
    28861 ctgctcgtac agcttcatct gtgatctcca ctccatggtg ctcttcatat cttgtacgta
    28921 atccttgaag aatcacttct gcttcttcag gagttggctc atctacttga atgcgagcaa
    28981 aacgtcgctc taatgcagaa tctttttcga tatatttttg atattcatct agggtcgttg
    29041 ctccgatcgt ttgtaattct cctcgagcta acgccggttt caagatattc gaagcatcaa
    29101 ttgctccttc tgctccacct gcaccaatta aggtatgcaa ttcatcaatg aataagatta
    29161 cttgaccatc attatagatt tcgtcgatga cctttttcat tcgatcttca aattcaccgc
    29221 gatatttcgt accagcaacc aaagatccca tatcaagcat catcaggcgc ttttcttgca
    29281 tgtcttctgg cacttcacct aaaatgattt tttgagctaa cccttcagca atggctgttt
    29341 tgcctacacc aggttcaccc accagtacag ggttattctt tgtccgtcgg ctaagaatct
    29401 ggataagccg tttcacttct ctcgaacgac caacaactgg gtccagcttg ttttcttttg
    29461 ctaatttcgt caaatcacgt gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc
    29521 gtttatcttg tacatttaca tttctacgac gattcattcc ttttccagca gaatcaccag
    29581 ctagccccat tttttgtttc agaacttgac gggcttttga aaggctgata cctaagttga
    29641 tcatgattcg agtagccatg atactttcat cttttaataa gctcatcaaa atatgttctg
    29701 ttccaacttt ttgagaacca gttttcttca cttcgctgct agctaaagca aaaacttgtt
    29761 tcattcttgg tgaatatgga agaaagactc ctttagggta agaacggata ccaccataac
    29821 caatcagatg ttcaatttct tcataaattt ccgtttcatt caaatccatt tcacgtaata
    29881 cttttccagc aattccattt tgctcgacca ctaaggctag taaaacatgt tctgaactaa
    29941 ctgattgatg tttcaaccgt tttgcttctt cttgtgcaat ttctaatact gcttgggctc
    30001 tttcagtaaa aagttcttcc atctaatctc acttcccatc ttcataactt aaacgttcca
    30061 gaaatgctcg gagaatccga gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt
    30121 tttttgcaat tgcactcaac atcaagtttc cttctttttt tgtaattatt tggtcttcat
    30181 aaagtttttg aataatagca aaagcatttt tctcactgat tgtatcatca aataactgat
    30241 ttatctgctc aagtaattgt tttttatctg aaatccttac ttttgcaatt cgtatataac
    30301 caccaccacc acgtttgctt tctactagat aaccacgttg gatggtaaaa cgggtattaa
    30361 tcacatagtt gatttgtgaa ggaacacagt taaagaggtc tgccatttca atccttcgaa
    30421 tctcgatcat ttcactattt tccagaattt tctttatata tgattcaatt aaatccgagg
    30481 tattctgatg tgccatctta ttcattccct ttgactaata ttgaccttaa ttataacgaa
    30541 tttgataata gaaagaaaga agaacgcttt ttgttaaata aatcctttag aatcaacaaa
    30601 aaaaaatacg gaatatacta ttccgtatc
//
LOCUS       DAYYCQ010000026        29960 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000026 DAYYCQ010000000
VERSION     DAYYCQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.026"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(256..1200)
                     /locus_tag="ACHAYF_001193"
     CDS             complement(256..1200)
                     /locus_tag="ACHAYF_001193"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8578645.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1496..2440)
                     /locus_tag="ACHAYF_001194"
     CDS             complement(1496..2440)
                     /locus_tag="ACHAYF_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8578646.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2661..3875
                     /locus_tag="ACHAYF_001195"
     CDS             2661..3875
                     /locus_tag="ACHAYF_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8578647.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3897..5912
                     /locus_tag="ACHAYF_001196"
     CDS             3897..5912
                     /locus_tag="ACHAYF_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8578648.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5995..7368)
                     /locus_tag="ACHAYF_001197"
     CDS             complement(5995..7368)
                     /locus_tag="ACHAYF_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8578649.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7394..7678)
                     /locus_tag="ACHAYF_001198"
     CDS             complement(7394..7678)
                     /locus_tag="ACHAYF_001198"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8578650.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7680..9695)
                     /locus_tag="ACHAYF_001199"
     CDS             complement(7680..9695)
                     /locus_tag="ACHAYF_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8578651.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9874..10179)
                     /locus_tag="ACHAYF_001200"
     CDS             complement(9874..10179)
                     /locus_tag="ACHAYF_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8578652.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10268..11299)
                     /locus_tag="ACHAYF_001201"
     CDS             complement(10268..11299)
                     /locus_tag="ACHAYF_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8578653.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11506..12381
                     /locus_tag="ACHAYF_001202"
     CDS             11506..12381
                     /locus_tag="ACHAYF_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8578654.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12533..13375)
                     /locus_tag="ACHAYF_001203"
     CDS             complement(12533..13375)
                     /locus_tag="ACHAYF_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8578655.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13467..15344)
                     /locus_tag="ACHAYF_001204"
     CDS             complement(13467..15344)
                     /locus_tag="ACHAYF_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8578656.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15341..16762)
                     /locus_tag="ACHAYF_001205"
     CDS             complement(15341..16762)
                     /locus_tag="ACHAYF_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8578657.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16765..20154)
                     /locus_tag="ACHAYF_001206"
     CDS             complement(16765..20154)
                     /locus_tag="ACHAYF_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8578658.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20411..21802
                     /locus_tag="ACHAYF_001207"
     CDS             20411..21802
                     /locus_tag="ACHAYF_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578659.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21875..22522)
                     /locus_tag="ACHAYF_001208"
     CDS             complement(21875..22522)
                     /locus_tag="ACHAYF_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8578660.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22530..23216)
                     /locus_tag="ACHAYF_001209"
     CDS             complement(22530..23216)
                     /locus_tag="ACHAYF_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578661.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23222..24295)
                     /locus_tag="ACHAYF_001210"
     CDS             complement(23222..24295)
                     /locus_tag="ACHAYF_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578662.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24517..25191
                     /locus_tag="ACHAYF_001211"
     CDS             24517..25191
                     /locus_tag="ACHAYF_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8578663.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25196..26536
                     /locus_tag="ACHAYF_001212"
     CDS             25196..26536
                     /locus_tag="ACHAYF_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8578664.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26685..27671)
                     /locus_tag="ACHAYF_001213"
     CDS             complement(26685..27671)
                     /locus_tag="ACHAYF_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8578665.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28395..29426
                     /locus_tag="ACHAYF_001214"
     CDS             28395..29426
                     /locus_tag="ACHAYF_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8578666.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29535..>29960
                     /locus_tag="ACHAYF_001215"
     CDS             29535..>29960
                     /locus_tag="ACHAYF_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8578667.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         8878 a         5614 c         5389 g        10079 t
ORIGIN      
        1 aattagatat gaatcattgt atttgccgtt gcggaaggat tttttaatta accacagata
       61 aacattgata acgattcgct tattgtaatc aacagattaa atttcatatg ttcatatgta
      121 cggatacatt ttcggaccga ttaattttaa ccattctatt tatttgctta cctattagat
      181 atcaataaaa aactgcttaa cagctatttc agctgttaag cagttttagg atttttagtt
      241 catattcatt gtttcttata atacagtgtt caatacttct cttaaagcac ttgttgattt
      301 gctgaacatt gctttttctt cttcgttgat tgataattca acaacttcac gaacaccatt
      361 ttcgtcaacg attgatggca cacctgtaaa gatatctttt tcgtcgtatt caccagttaa
      421 ataagcagat acaggcaata cagcttgttc gttattcaag atagctttta cgatacgtgc
      481 agtactcata ccaataccat agtaagttgc ttttttacga tcaatgatct cataagcagc
      541 attacggact ttatcagcaa tcacatctaa ttcatcttgg gcaatacgat ggtctttttc
      601 tacaatttca aacactggtt taccaccgac agttgtatgt gaccatgcag caacttctga
      661 atcaccatgt tcacctaaga tatagccgtg gacgctgcgt gggtcaactt ttaatttcaa
      721 tgcgatttct ttacggaaac gagttgtatc caatgttgtt ccagtaccga taacacgtga
      781 tgttggcaga ccagattcgt tccaagcgat atatgtcaaa atatctactg ggtttgaagc
      841 aacaacgata ataccgtcga atcctgatcc catgatttct tttacgattt ggcgcatgat
      901 tgacgcattt gttttaacta aatctaaacg agattgacca ggtttttggt tgataccagc
      961 tgtgataaca acgatatcag catctttaca ttcttcatag ccacctgacc atacagccac
     1021 attttcatcg ccccaagcca taccatctaa aaggtccaat gcttcccctt ctgctttttc
     1081 ttgattcaca tcgatcaata ctaattcatt agagattcct tggttgatca ttgcataagc
     1141 gatacttgtt ccaacaaaac ctgtaccgac gattactact ttacgacttg tttttttcat
     1201 gaataaacag ctccttttca taactatttg ttactttcat gaaaagtata gtcttgttta
     1261 ttcattttat caacatgtta attataatag ttgttattat aatttttgat ggaaacgaat
     1321 tcctgaatac gcataacaga taaatgacaa atgtttacac ctatatcccc tgataataca
     1381 accaaaagtt tagaactgaa aataaatcca aaaaaattcg ctgcaacgat ataataagac
     1441 agcaatcgca cctaaaacaa ttttccttca tatgctttag tgaaagattt tcttcttaat
     1501 gtttatttct ccattcattg ggagtacatg ttgaatactt cttgaataat ttgaataaag
     1561 aagtcgtttg tttatatccg atacgattag cgatttcttc caacgaaaaa ctcgtttctt
     1621 gaatcagttc caacgcaaag tcgaattttt tctttagaac cagttcttga aaggaagcat
     1681 caaaatacat tttcaattta ttactaagat agaccggatt gtagccaaaa tgagctgctg
     1741 tttcttttaa cgtacaagaa gtaaaatgct tatcgatata atctaatact ctaagcatct
     1801 cattttctgc gcatccatct gctgatgaaa agctttgttg ttctaataaa tgagtcaatt
     1861 cgatcagtaa tagtgatgta tagagattta acgaacggat cttatgactg tatgtatcaa
     1921 aataaatcaa cagcatattt tcaatcaaat gcgtcaaacg acttgttttt ccgccgcgaa
     1981 aatgaatgaa ttgtgtatgt tccccttttt cttttgatgc atttatcaaa aaagaagaaa
     2041 gtagattatt tgtgcttgcc aaatgattca atatctctgt agataaggtt tcttctttta
     2101 ataagatgtt gattaaaata tcctcttgcc ctgtgtattc gattctttga gtagcttctt
     2161 tatcaatgat caccacgtca ctttcttcca tctctatcct ttctccatta ataatttgta
     2221 aacatgtacc gctatacata taattgattt caacaaaatc atgtgtatgc tcgggaactt
     2281 ccgcatatcg acggtgttta ctgatatgaa tatttccctg ttgaaataga tcccctcggt
     2341 ggatttttgg cggtgtctga agatggtaag ctaagtttaa atcattgata ttcattcctt
     2401 gttcacgctg cagagtttcg attgattctt cgttacgcaa aaaaaatgtc taattcagct
     2461 agttccaatt gactccctcc tttttgtaag cggtttcttt cgcttattag ttcacgctgt
     2521 actgctatta tatgcaaagc tatttttggt ttcataaaga ctttctatgg ttgggacatg
     2581 aacttcctgt gattccgcta ttgagagaat aaccacttca tactatattt ttgataagcg
     2641 cttacaaaaa tgaaaggaac atgcttatga ataaaaagat gatgaaattt tctctgctgt
     2701 cagtctctct tattttgact tctgctggtg cagtcacgtc tgcaattccc agtatgtcag
     2761 ctacttatgc cacgcgttct ttatctgctg ttgaattatt aacgactgct ccctctttta
     2821 tggtcactat ttttgttttg attagtagtt ttattgctag gaaaatcgga caaaaacaaa
     2881 ctgtgatcat cggattagtg attgcatcga tttgtggtat cctccccctc ttctcacaaa
     2941 gttacagtat tgtcttagct tcaagagctg gactaggtat tggctttgga ttgatcaatt
     3001 ctttggcagt cagcatgatt gctgcctttt atcacggaga tgaacgagcc tctatgattg
     3061 gttttcaaag tgcatttcaa ggatttggtg cagctttaat gacatttgtt gcaggacagc
     3121 tagtacagtt cggttggcaa tatacctttt tagtctacgc catcagctta cctattcttg
     3181 tcttgtttgt tttgtttgtt cctacacctg aaacaactga acttcaatcg catggcacag
     3241 ccccaacaag acaagtaatc aaaacgaatc ctaaaatgat tagttatggt atttttctct
     3301 tgctggtcat cgtggtatat aatgctgtat cgattaaatt agccacagtc ctgacgactt
     3361 attctctagg aaacgaaact aatgcagcga atatcttaac aatcatgcaa ttggcaagta
     3421 tggctgccgg agtttgtttt ggctggcttc agacaaaagc acatcgctat atgcttacac
     3481 tttctttact actgatggga atcggctttg tattgattgc cactgtgata aacctgtatg
     3541 tgattgggtt tggtgcgatc ctaacaggaa tttctttttc gttattcatt ccttacttat
     3601 tcaaccaagt aagtatccaa gcgcccaagt acgcggagag tttcaatact tcaatcttat
     3661 tggttggcgc taatttaggt actttcattg caccatatgg tttgctcttt ctctcttttg
     3721 cttctcttgg gagtaaaaca cttgctgttt tcgtcaatgg cagtatcttg ctgttgatta
     3781 gcgcgcttat tagccttcta gtagtacaca ggacgaatag aaagacagtg ataagagtag
     3841 atgaagaaat aaaagaacaa ttgacaacaa aataaatttg atggaggtcg tgtaaaatga
     3901 caaatgaaaa acataaagat agatggcttt ggtatcctgg agactttgag atccgccatg
     3961 gactgctaca aaatttccaa cgggaagaac gaggttttga ctggccagcg tactggtaca
     4021 tggatgattg ccatcggaac gtcaagttta agagatatta ctttcttgat caaccttcca
     4081 tgtttaaagt gacgatccaa ggagttgggt atgtagaaat taatggacaa aaacatccat
     4141 gtggaaaatg gctgacttgt ccagctggta aagcaaaaat tcgtatcttt gtaggacata
     4201 catcagggct accagcgatg tttatcgagg gagacgaagt aaaatcagat attggctgga
     4261 cagcaagtaa ttttattgaa gaatatccag ctggctggag tccgctttat gtcgatatcg
     4321 caaaagatcc aaatcaaatc tattatcaaa aagaatgcgt ccatccagtc actgaaacgg
     4381 agattggcgg aggaatctta ttcgattttg ggcgagcagt gaatggctgt gtagaactaa
     4441 tatctctgga tgaccatatc gaatctatca ctctatgtta tggagagtcc gatgtagaag
     4501 ctatggatat cgattattgc tactataagc aaataggaat gacaagaaaa atgaaagctc
     4561 gtaaacgcgc ttttcgctat ttgtttatac cagaggtttc tactggaatg gttcaacttc
     4621 aagtttttca tgaattttta tctctttcga aacaagctgc tttttcaagc gattcttctt
     4681 tactgaatca gatctggtca gtatcagaag aagccttcaa gttatgtagt gatttgtttt
     4741 ttatcgacgg tatcaaacga gatcgctgga tttggtctgg agatgcgtat caatcattca
     4801 tgatcaatca atatcttttt tttgacgagg aaatcaataa acgaacgata ttagcattaa
     4861 gaggacaaaa tgaaatcaag cagcatatga atactatcgt tgactattcg atcttgtggt
     4921 tgattggtat cgaaaaccac tatatgatga cgaaagacta tgaattttta cgcatcgtct
     4981 accctaagat gcaatcatta atgagatact tgatggagca aacgaatgaa ttagggttta
     5041 tttatgggag agcaaaagat tggatattta ttgattggtc tgaaatggat aaagaaggaa
     5101 cgcttgctgc agaacaagtc ttattactga aagcctatca ttccatgatc acttgcggag
     5161 atatcttagc tgaaacatat ggagagaacg aaaattatca tactcatctc tatcaagaaa
     5221 aatatcaact attgaaacaa aacctatttg actacttttg ggatgaagaa cttggcgctt
     5281 ttatcgattg ctatgaatct gggagaagaa atgtgacgcg tcatgcgaat attttcgctg
     5341 ttttattcga ttttgctaac aaagaccaac agcaatcgat cttaaagaat gtcttattga
     5401 atgacactat cacacagatt actactccat atttcaaatt ctatgaacaa gatgctttat
     5461 gcaaactcgg acagacgaat atcgtctacc aaacgattct tgattattgg ggagggatgt
     5521 tatctcatga tgcggtaact ttctgggaag aatacgaccc tactcaaaca ggaaatgaaa
     5581 agtacagtat gtatggagat ccttatggaa aaagcttatg ccatgcatgg ggagcaagcc
     5641 caatctatct tttaggacgc tattttatcg gtttgtatcc aacaaagcca ggttatgaag
     5701 aatttatgat acaacctaat ctagattcat ttgaaacact gaattgtacg ctacctatca
     5761 aagatggaaa gatttcgctt gaaaaaacaa aagataagat cgtgattact tctgatagaa
     5821 caggcggaac agtgaaaatg aatggtaaaa gttatcctct tctagcaaaa cagccacttg
     5881 tcttaacaag ctattcaaaa ataaatgcat aaaacaaaaa actgcttgcg acatatcaga
     5941 agactttaaa tcttcttctg atattgtcca agcaattttt attttgaagt ttgcttaatt
     6001 ctgttcttta gaaacaatgt ttgttctttt tccggcaaaa agaaagctag ataatacgac
     6061 aagtaaaaag aaagcgatga ttccaactgt caaggctatt tgaccgtatt ctcctagttt
     6121 cccaagtaca agtcctggta ctagataatc agtatcggca aatgtagaag aagcgatttt
     6181 aagatctcct aatactggaa ggataaataa tggcagccaa ctaatcaata agctattgaa
     6241 agcagaacct aataccgccc ctcttctacc gccagtcgag ttgccgaaga caccagaagc
     6301 agctccgcag aagaaatgtc ctactacccc aggaatgata actgtagttt gcaagcctat
     6361 catagcaaac atggatagtg ttcctacaaa gaagctaaca aaaaagccaa ttaatacagc
     6421 attaggtgca tatggaaaaa cgataggaac atctaatgct ggtttagaat cgggtacgag
     6481 ttttttagca atcccttgga atgctggaac gatttctgcc aacaccattc gaaccccttg
     6541 aaggacgata acaaacccag ctgtgaatgt tccagcttgg atcagtgcaa agattacagc
     6601 gttgtctcca ttgcttagtt ctctttcaac aaatccagga ccagctaaca aagcaagtgc
     6661 gacatatacg atcgacatca agagcatgat cgatacagta gaatctcgca aaaagctgaa
     6721 acttttagga atctttatgt cctctgtaga acggtctttg tttccaaaaa actttccgat
     6781 ccatccacta gaagcataac cgatatttcc ggtatgccct aacgcaactt gattgtttcc
     6841 agtgatgttc ttcataaacg gttgacaaag agcaggcgta atagttaata gtgttccttc
     6901 gaagaccccg cctaataaga tcatccacaa atttgcagta aaccccgcgc ttactaaaat
     6961 gactgctgtt agacaagaaa cataaagcat tgcttgacct gtaagaaaaa tgaatttata
     7021 tcttgtgaat ctagcaagta aaatattcac gatcatccct actagcatga tcaaagcagt
     7081 aggtgtaccg tattgttgca aagcaatcgc cactactgct tcattgctag gaactactcc
     7141 ttgagtatgc agagctgttt ggaacatcga agcaaaaggg gttagtgaat tactgattaa
     7201 accagcaccg ccgatcagta ctaggaatcc gacaaatgtt ttgatagttc cttggatcac
     7261 ttctgaaaat ttcttcttct gaagaacgag accaagcagt gcaatcaaag aaacgaggat
     7321 ggagggctga ctgacgattg acacaaacaa attcaacaat ttatccattg atagttcctc
     7381 ccttgaatca tttttattct tttgacaaga tcttcgttac tttttctttt acttcttcgc
     7441 tgtcaatgat cgattgcaaa ggtacgagct tttcatcagg tatagaagtt agcgtatctt
     7501 tcaaagaatg ctcaatgaag aaataatcag ccatatcgct agatacactt ccaacatccg
     7561 tatgacttac ttcaaactcg tccacatctg ctccaagatc tgcaagtgct tgtttgatgt
     7621 tcatctctac cataaaactt gttcctaatc cactttgaca tactgcaaaa attttcatat
     7681 cattcgtctc cttttcttat gatcgctaat atttcttctt ttgttgatgc gtgtaataaa
     7741 tcttgaagat tttgtttatt agaaagtagc tttgttaaat ttgccaaagc ccgaagatgt
     7801 gcctcattat cgatagcagc taaacagatg aacaaactaa tgggatgggc tggattatca
     7861 agcagatcca caggttgttg aagtttcagc aaagacatcc ctacttcgtt tacgccatct
     7921 tctggacgag catgaggtaa tgcgatgtga tcgccaatat ggataaacgc accatactgc
     7981 tccacgcgct cgatcatagc tttgatataa ctttcttcta ttttattttc tgataatagc
     8041 ggctgtgcgg caaatgtgat tgcttcttgc cagttttttg gttgatccga taattggatc
     8101 atttcttctg ttaataattc agatagcata ggacgttgat cctccttctt ttctaaaagt
     8161 ttgttcattt tacgatttaa tacttgatag agcttttctt cgtctacgcc ttcttttaat
     8221 tcgatataag gcagtagcgc cgacaacaat tcttgggcag aaggcattga aaatcctggt
     8281 atcagccaat ctctttgtac ttgattgatc agctgatttt tttccaagtc agtcatgatt
     8341 ggatgcagaa catagagaga ctttttctta tcagtaagtg tgagaggtac agtagaaaaa
     8401 ataatatcgt aagaagtttc gtcaacctcg tgtaagcggt cagctgaatt agtctctgta
     8461 aaaaagatca tcgggaataa ttgctgaagt tcacttttta agatcaatga agagctaatg
     8521 ccacttggac agacgatcac ggctcttatt tttctatttc tttcttctgc gtccgctttt
     8581 ctgatctctc cgccaaatag tatagtgaaa aaaccaatct cttcttcagg gatgctcttc
     8641 cctattcgtc tttctaacgg aagtaatgct tttcttgtca tttcgtagat ggaagcatat
     8701 tgatgcttaa tttgatcgat catcacatta ggtaaataaa aagaatagga aatacgaaaa
     8761 taggcaggga ccaagtgata aaagacattc atcaataatt cacgaaacga agcaaattct
     8821 acggccatca ggaattccat gcgatggatg acttctgaag aacattccag caaaaaatca
     8881 agtgcaggct cctggacatc tccttcaacg atcgatgcta agcaaagaga gaaaaatata
     8941 atctccgaat tttgatgttc ggaatagcca aggccagtca atacttgtcg actaatgtca
     9001 gtgactgcaa gagaagccaa cagttcagca tcttttacca gttccatgga tgtttttcca
     9061 attcgaatgg ttgtcaaact caaataataa gctaattcat ccattcgatt tggtgcgaat
     9121 cgaagatctg atagtgaaat cgttttatct agcaaatctc taactttggc gtaataatca
     9181 atggaaaggg ttgcggttaa atacgtgagc ccaaatcttc cagcaagtga ctgcatcaaa
     9241 cgagcagtgc gattttttgc ttctcttctt aatattgctt ctgaccctag cagcctaaaa
     9301 ccagttttcc tagtatacga cagctgacaa tcttccactt ctaattcatt tcttatcttt
     9361 ttgatatctg acaggactgt ccctttagaa acctgaagaa aatcttgaaa atgaaaaaca
     9421 gaaacagacg ttgcagtcga ataaaccatc aaataaagaa gatctcttcg ctcttgttcg
     9481 gtatacacag cttcaaatcg agtaatatcc aaaaattttt ctgcccagtc gattgctcgg
     9541 aatttcttta tacggatacg ggtatcatcc acatattcgg caaaaggagt taattgtttg
     9601 atcgcctcgc ttgtatttac ttcgatgatc tctgctgctt tttctagact gatttctctt
     9661 ggtgttttca ggagataact aattttgtag ttcacagcgc tgttcacctc ctacttatat
     9721 tctagtatgg tagcgctttt aaatcgaggg gtgtttggta caacttacag ttggattttt
     9781 tttacgactg taccgaacga tttctatcga atggatacag aaaaaaagca ggtgaccaaa
     9841 tgtctcctgc ttttatgaat tgatttcgtc cgatcaatga tgatcatgtt tattttttga
     9901 ggtcagttct tcaaattttt ctttgacatc ttcaaccgct ttttcgatat ctttttcaaa
     9961 gtgattgggc tcattaggct ttgtttcctc ttctacagaa tttgaaccag ataaagctgt
    10021 ttctttattt tctgtttcac cagagtttgt gccagctaat gattcttctt tgtcttctgt
    10081 ttcaccagaa ttagttcctg ctaaagccac ttcttcttct gttttagggg ccattgaacc
    10141 tcttaatcgt ttgtgttttt caccgtcata atttgtcatg ttggttcctc ctagtagttc
    10201 tatacttttt catcgtacaa taagacagtt tatgcgtcaa aaaacaaacc cgattcttac
    10261 aaatatgtca tatttttcct tctgattctt gaatgctttt gattccttct tccaaaattt
    10321 tcatttgtaa gattgtttct tcatctttga tgactttttc tgcgccatta actaatgtct
    10381 cgtaaatccc ttcgtagaca cggctgtaat caccaacctc ggaaattact ttttcttcat
    10441 gataactccc ttgttcatct atataggtca atacaccata atgttcgggc aaatcgatac
    10501 caaagtcttc atgatcaggc atgtaaaaca ttttcagatg ttcttcttgt cgatcttttg
    10561 tttgtttcac gaacatccct ttttttccgt aaacgacgaa acttggacgt tctttgatgc
    10621 ggaaataact ggattttatc gaaattttca tatttccata gaaaaaatcc agatcgaagt
    10681 aatcattcat tcgtcctttc cctaaaagtt gacgtacatc atagctgata tcatctggtt
    10741 cgccgaaata agaaagaact tgatctactg tatgacaagc atgtccatat aaatagctgt
    10801 tcgcagtaga aaattcatgc gtattttctg gtacttccgg tctgaaataa tcataatgca
    10861 tttctacttc taaaatatcc cctaattttc cgctttcgat tactttttgg actgtcaaga
    10921 agtcagaatc gaaccgtctg ttttggtaac attggataaa tagacctttt tcttttgcta
    10981 atgcaaataa ctcttctgct tcctttgacg tttctgcaaa aggtttctct actagtacat
    11041 tcttaccatg ttcgagtgcc atttttccat attcataatg aaattgacta ggtgtagtga
    11101 tgacaactaa ttggatctca ggatcatccc aaagatcaga cagctgatca gtatattcga
    11161 tgtcttcgaa tgttttccac actggttcag atggggaaaa aatttttttg atattgattt
    11221 tgttcgtaag ttttcttgaa aaaggcaaat gatagcgatt tgtactcttt ccattgccaa
    11281 gataagcgat tgtaagcatg agtggtgcct ccttttattg atgttttcag tatatcctag
    11341 aaaagcgcag aaagtattta taatagctat tctggtaaaa aagttcagaa aaacacttgt
    11401 tatcattcgg tttggtgaaa ttaatgttca aagtaaggat aaaaatcaaa aagatagtag
    11461 cggaaccaag tgtttatcag taagatagaa tttggaggtc agatcatgct tattgaagaa
    11521 aaactaacaa aacaagagtt gtttaccacg acagagaaac ggattgtcga ttacatccgt
    11581 aggaatatcg aagctgcagt ctacatgacg attgaagaat tagcaaaagc tacgtatact
    11641 tcccattcag ctatcattcg tttatgcaaa aaaatgggat tcagcggatt taaagaattc
    11701 cgtttcgagc tggggcggga agttcatcaa ttgattgctc agttcaatca aacgattgat
    11761 cctaattttc ctttttcaag tgaagatagc actcagcaaa ttgcaaagaa aatggcggat
    11821 ttatcgatcc agtcaataaa aaaagcacaa ttgcagattg aacatgaaga tttagatcaa
    11881 atcggtaaag tattgaacaa ggcacagcga atctttcttt ttgcaaaagg tgattctcaa
    11941 atcacggcaa ggaaattcca gaataaaatg gtcaagttga ataagttttt gatcatggct
    12001 gaagaataca gtgattcttc ttggaatgca gccaatttga ctagcgagga ttgcgccatt
    12061 tttattagct acagcggtcg catccatcat tatgaacgaa taatgaccta tttgaagcat
    12121 gtagaggccc ctaccttgct gattaccgga aatcagcatt cagaaatggc caaacaggct
    12181 gatatgtgtc ttgttatatc gcaggaggaa tatgattttg ctaaagtagc gactttctcc
    12241 tctcaaattg ccttcgatta cgtattaaat acgttattct cagtgatata ttctcaaaat
    12301 tttgaagaga atatccgcaa cttgaaaaac aaacaagaat tgatgcaaaa aggccttctt
    12361 aaagaatatc ggaaaactta agaaaagatc ctcatttagt aataagaaag atagtgtttg
    12421 ataggctctc tttgatatca tcataaaaac aggctgaagt tttctttttc aaacctcagc
    12481 ctgtttgttt acggaacgat tcgaatagat atattataga aatgaggaat agctaacttc
    12541 tgtttcttat tttgaacgat tgacgaaaaa gatatataag aaggaggaaa atcagtatac
    12601 atgcagctaa caatccatac aggaaaaatc gtttgttctc ggatactttc tgtacttctt
    12661 tttgaacttc ttttgcatga taaggaaccc ggtgccctct gacaagtaag cgatgactgt
    12721 tgaccatata aggtgtacaa gttaggagtg tcacgaaatc ctgccctttt tcgattctca
    12781 gatcttttgt atcagtcggt tctaccgtct tgatctgatc gacttgataa gcgagtgtcc
    12841 gtccattgat ttcgatatag aaagaatcac ctaaagtcag tttcggtaag tctgtgaaca
    12901 actttgcttg aggcagtcct cgatggctgg aaataacggc atgtgtattt tctccccccg
    12961 taggataaga cgttccttcc aataatgagg cacctttttc tagcagcaca gtattcgttt
    13021 catcaaaaat tggcaaatgg acattgatct cagggatcgt caaaataccg atcgtatgtt
    13081 tctcaaaata ggttctatcc tttttcgcag gttctttttt cttagtaaag ggatctgcac
    13141 ctggatttcc accttctttt gctaattgct ggtttttctt tgtcatgttt tcttggattt
    13201 tagccatgac ttcttcattt tctttgaccg cttgttgctg ataatgagag atgatctgct
    13261 gatccagata attattcagc gcgtcgctaa caaatggata agctagtatc cctattcctg
    13321 agagaagcaa gaaaagcatc agcagatcta gaagtttcct tttctttggt ttcatttgtg
    13381 ttctccttgt gatgactgtt tgataataaa agaaaaagga gcatttgcgt aatccgctgc
    13441 tcctttttcc aattcttgca aaaagactaa atctgactgt gcttgcgtct agcaaagtac
    13501 agtccagcaa tcagtagaag gactactcct gcaccgatat acacatagat tcccttaccg
    13561 cctgttgaag gaagtgtacc tttgtgttta tttggtattt tttcaggaga aatcaactgt
    13621 cctgctgtta cataagattg ttcatcgata gtaaatgcta cacggtttgt taatggaaca
    13681 tatttttctg gcgctttcgt ttcttccaga tagtacgtgc catattttag acctgtcaca
    13741 tcgattaaac cgttacttgt ggttgtaaaa accgttgctg attctttcgc cgatacccag
    13801 ctgacggctt ttgtggatgg gtcgatcgat aaatatttcg ctgtgtcact atcttgatca
    13861 cgaacgacga attctgctcc agcaagtgtt tggtctgatg taacgtcacc atctacttta
    13921 acaaatcgtt tgcccccagt aacgacatcg actgacggtg gtgtttgatc atttgtatgc
    13981 ccgttatcga catttgcttg gttagaaaat cctttggttg gatctgcttt ttcgttcaaa
    14041 tgcatatagt aaacgaattt caatgtaccg ccaggagtta atgaaggaat ataattcgga
    14101 tcaactgaaa ccgtgaatcc gtctgtttgc tcagtgacag aataatttac tgggtcgatt
    14161 tctttatttc catcatataa ggcataagca tacgttcctg aagaatcatt atcaaatgtt
    14221 aaagcagcgt catgagtatc gataagtttg aatgttgtgt acttgttttg cgttccttct
    14281 ttatcagcga tacctaatgg gatattgaca gaaatctcat attgaatttt ttcaccgatt
    14341 gcgacatctt ttccattcaa ttgaggtgtt gttttatcaa ctttagacgt atcatttttg
    14401 atggtctttt ctactggtgt ttggctattt gccaggatct caaaaggcgt gattgtttgc
    14461 ttttcaatca tttccgcatt atcaggagct tttacttctt ctaaattata ttttccaact
    14521 tctaaatgat taacgatcaa ctggcctttt tcaatagatg cctcggcaaa gtcattgttg
    14581 ccgatgtcat aagaatgacc agtaatgaaa tgtttggctt tggtttgatc agttgtccaa
    14641 gtgtacaatc catctgtgac actttggatg tattttttga cgcttggtgt tccttcttct
    14701 ttagaaataa taaattctgc tccatttagt gcttcgtttt cggcagtacc gatttttgta
    14761 actttcaacg ttccatcatt accgactgta tttttagggt agagatggat agtatctagt
    14821 tcttctgtcc cgtatttata agagccatct gcttgtttga tcatctcata tacagggaaa
    14881 gctaaaacca tgtttgcggc agctgacact ccgtcttttg gttcttcttt gatcgtgtag
    14941 actgcatctt tcccattttg ttttttaggt aaagataaag tgacatttcc atcagcatct
    15001 gtcgttcctg aagcaactgg tgtaccagga gtcaaagact ggactgcttg ttttgctgca
    15061 tccacggacg ctcctttatc tcgttgcgca taaaattctt gagtgacgtt ataaactgaa
    15121 aatgaaatat cggctaatcc ttggtattga tcgaattcgc tcatttcttt cccgctgttt
    15181 tggattaaag gatcgggtaa atcagtcatt ttctttttat gaaggatgac ttgagctgct
    15241 gtctcctctg caaacacttt ttttgcgccg aagctgtttg tgagtaatgg taaaataagg
    15301 aaaaggactc ctaacataac gtttattttt ttatgatttt tcattttttc actcctgttc
    15361 tttttttgat aaacaaccag attgcaaagc ttgcgaatac cataccaaca acgcctaaaa
    15421 gtgtattttt cgtttcgttt gtttttggaa gaaatggacg gctattttct tttttattga
    15481 ctactttcaa tatactgtcc gcctctttat cctcagaaag aaaagttgaa atggtaaacg
    15541 gaatttctgt ttcacttaaa atataaccag aaggtgcttc gatctctttt aatcgataac
    15601 ttcctgtttt caacccggaa attgaaaaag cgccattttt atcggaaatc aggtgaagcg
    15661 catctgattc gttctttgtc cacttgtatc cgttggctgt ttgctggagg taatttcctg
    15721 ctttgttttt aataaggaat actgcgccag ctaatcgttt atctggttta tttgtctcga
    15781 ttttgacaaa ttgcttactc cctgtatata cagagacttt ctttgtactg acaccaaaac
    15841 caaaatccag tgttccgttg ttgttgattt ccttgttcgc ctgtgctgta ctgcttagct
    15901 gcatctgata agaaatggtc agtttttttc cagcatattt ttccaacgaa gggatcgaaa
    15961 ataaaacatc aaatccgtga ggtttcttgt gtatgacaaa gccttctgtc agcttttctc
    16021 cattcgatga aatcgttaaa ctattaggta aaaacgttaa tgcttcatcc gcactatctg
    16081 acaattcgaa cttttgatag tcaaggatat ttacaggaat tgtcgtatgc aaggaataag
    16141 tgattttttc accaatcgta aaatcatttc taggctcttc taataccttc tcaaaaggtg
    16201 gatcagggta gtcgttttct tcatttttag gatacaaatg gatagttgaa agtttttgat
    16261 tgttttgtcc atgtacagga agaacaacta ccatattctc tgccttttct ttgacgactt
    16321 ctggtacttt ggattcaatg aaaagatacg ctttatctct tttttcttga tctttagcgg
    16381 ccagagaaaa agaagcgata ccatcttcgt tgttaagagt tgtagttgtt gcttctgcgg
    16441 taaacatacc ggaagacgca ccgttttttg cgatctctgc ttgtgcttct tctaccgttt
    16501 tgcccttttc ccgtagatgg taaaaagaat ctgtgacatc ataaacttgg aatgtgacac
    16561 catttaatcc tcgatacgtt tgtaataaag ctttttcttg tccgtcattt ggatgatttt
    16621 tcggcagttg cccgtttggg aaaagcaatt tgtgtaaagt aatctgaacc atttctgttt
    16681 ctgtttctgt tgctacctga gtaaaagctg gtttaaatag taacaagaag agacacatac
    16741 taagccaacc aagttttttc atatttagct gccccctctt tcttttttca aaaaaagata
    16801 ccaaatagaa aacgcacaac caatcatccc tactagatag attcctaaac ggccaattcc
    16861 tcccgtttca ggtaatggta cttttgcctg attcgtgatg tctaaagaaa tctggttgtt
    16921 tttggctcct ggtgacagaa cagattcatg atcttgtcca tccacttgaa ttgacccatc
    16981 ttcgtgtata actatagtaa ctggctcttt tagaccttgg tatccttctg gtgtaaaagt
    17041 ttcggtcagc gtataagttc caggttttaa attttcgaat agaaaggtat ctgtttcttg
    17101 tccatctttt ggcgattcta agtccatttc tggtccctgc agtctgaatt ttgctccttt
    17161 taatgtctgt ccgttgtctt cttttttgtg taccgttaga tcaaacgctt ttagtcgatt
    17221 ttgatgagtg agcgtccata cattatttgt catctcggta ttttttcccg tgtaaatgat
    17281 ttttccatat ttatcgattt ggaattcata atttcccggc gaagtatcat atccggcagg
    17341 accagctgtt tcaacgactc gatatttacc aggtgtgagt gaatcaaaac tggcaagacc
    17401 tttttcattc gttgtttggc tgtcaattgg ctgataagta ccatttgctt cttttttctg
    17461 tagggaaaat gctgctcctt taagatttat ctctttgtcc gtcccttgca tcgcatactt
    17521 acgtactgct acaggaactt caacaaaagg attttcaata gtcaactgga tcgtatcatc
    17581 ggaagtagtg actgtttttc catcaatggt taccgtacca tcagaagcga tcttgatttc
    17641 ccaagtagtc tttttgctta atccatgccc ttctggagct tttgtttctg tcagcgtata
    17701 ggtcatttct ttttgcaatt tgacattttc tggaagagaa taggttccgt cgccatgatc
    17761 tgttagtaaa gtatcaatag aatctcctgt taatttgaaa acagcgccaa taagatcctt
    17821 ttcacctgta gaggaatttt tcgttatttt caagtttaac ggtgtgaatt gtttagtatt
    17881 tttccatgtc attgcatcta tttgttgaga atcgtatcct ggtacaggta attctttgat
    17941 tgtttggtaa ctgaatgctt gaccttgatt attgtattga ggtagtgata atatctcttg
    18001 ataactttct ccgctatttg cagataattt gtcaatgtct gcacgttccc acgtatttgt
    18061 tgtatcttta gctggtttaa tgattcgaat atatccgttt ttccaagctg cagcatttgt
    18121 cgtatgttcc cgttgaatct caaaagtaac ttgatctgga cgatcagcta gattgttgtc
    18181 aaattcttcc cataactttt tgatgtgaag actgactccg ggagctttag cagatggtat
    18241 accgaattca gctaattcat tggtatcaat gtttggctgg aaagttgtcc tgccgttcat
    18301 ttgataccag aaatttggat ggaagtcctc attttctgtt tggattctca cttggtaatg
    18361 gatttggatt tcttggtctt ttcctaaata gatctgattg ctcttgatgg tatttccttc
    18421 tatggaaata gatggagtga ccgttgtagg acttgtcccc acactcttga ctgaaagtgt
    18481 accaggctga taaatgaatg gttctgcgat tggatcattt atttgtccat tgcttacagt
    18541 tgctgagatc tgtacagctt ttttggctag gtattcagag atatctgttg catgatcagc
    18601 tgattcatag taaagatcgc ctttttcatc tgatgaaacc atttgtcgca tacgagactc
    18661 tacttctgct tttgagagac cagctgccgg atcgctttga agttggatgc caagaccatg
    18721 tatttcgatt cctcgttctt tgagtgccat cgcttctccg atggttgcga taaacgtact
    18781 gtcgattctt ctggatagat tgttttggtc aggtgcatca tagattcttg agattagaga
    18841 agtatttccc ggccgatctt gcgtattaga aaactgagtt ccgtaataat tgctgcttga
    18901 ttgtgcgtgt acccgctgta ctttatagga aaatgttggt acaccatccg tcagcaaaac
    18961 gatcactttt ttatgtccat ttggaacgga tagcatgctt cctgcatctc ttagtgcttt
    19021 ttgtgtaaaa gtaccaccat ttgtccgtga aggtgtaatg gattttactt gatttttcac
    19081 tgaatcaaat gaacccatct gtactgcacc gttactgtag ctatatcctt cgcttgagta
    19141 gccgacatat cccatattga ttttgtctgt gataccgcta tctgctaaag tatcgacaaa
    19201 acgatcgaca ccaatcttta cttcaccgat ccgattattg tcgttcatac ttcctgacca
    19261 gtctacgacc aatacgagat caagaggcgt gatatctttt tgtacatttc cacgagcatt
    19321 caaataaaca tcaaacaatc cgggtgtact tgtttccttt gcatactttc gtatcgcata
    19381 gtctgcttga ttaccggttc ctccatattc gatatacgaa tgggtccgat cattaggatc
    19441 tccattccaa gaatttatac catcccattg atttgttccg tttttattgc cttgatgatc
    19501 aagaacattt gtatttcctg taggttgcca actggcttta ggataagtgc cctgatcatc
    19561 tgttgtatat tctgtaggaa tcgtttgatt agatggcaca ctcacagctg gcaaggataa
    19621 tgtaggcaat gtaaatgggc tgacagaatt ttcgttttct tttgatgatt cttcttctat
    19681 ttcgtctggc tttggtactg acacatctgt atctttctta ttttctgtct catcaataat
    19741 gtcatattct ttattatcga cgatcagctt gtttgcctgg ctgtggtaaa gtgctggatc
    19801 gattggaaaa ctcagatcga tagtttgttt agtgtttgat gagatttcca acgtaatttt
    19861 cccgctattt acctgataag catagccatc tccttctttt ctttccacat cacttaagta
    19921 attttcgact agaccaattt ctttttctat cttttcagca ctggtgttat tcgcatttag
    19981 cttgatgttc agtgcttttt ttccagactg atccgaaatg acttcagctg aaattgtttc
    20041 taaaactgtt ggatcatctg tagtgtctgc tattgcagat aatggcacaa agtttgataa
    20101 actcaatatt atcagcatga aaataacttt cagtttcttc cctgtggtcg tcatttttcc
    20161 cctccttttt ctttttttct gttttgacgt gaatagtgaa aattattaaa ataaacaggc
    20221 gttacataat tttatttatt ttaatatgat aattattata cgaagggcgc taaagggaat
    20281 cacaccaatt ttcataccaa ttggtttcat ttatgaagtt gattccttcg attaatgagc
    20341 gtttcattga aagaaaaggg ttgagggaga aaaacatatg atcaggttgt tggaaggaaa
    20401 ctatagaaag atggaaaaaa tcattcgaat tctgtccatt tctgacaagt ggatgactaa
    20461 aaaagaactt gccaaacgaa ttggtagttc agaatctaca tttgtaaggt atatcgaaga
    20521 gatcaaaatc cgctggggaa cggatgtaac gattcaatct tcacaaaaat tggggtatcg
    20581 tcttgaacat ttgaatgtac cagcttattt aaatgttctg atggaaatgt cgcaaagttc
    20641 tacaaacagt cgtttattaa atgagattat tgtcaatccg ggtaaaacga ttgagtatta
    20701 ttgtgaaaaa atttccatca gccggtcttc ttttacacgt aagttgaaac aatgcaatca
    20761 aattttagaa agctatttat taaaagtgat cgttgaccaa ggttttcagt taactagcct
    20821 taaaagtgag ctccagttgc gtatctttgt tacctttttc tttttaagtt actacggaag
    20881 acgcacgttg ccatttccaa tcgaaaaaaa gaagatcaaa gaaattatgg tcaagaataa
    20941 ttgtcagatg aatttgtttc aggaagaaag cccatatgag caatctttct ttattatgta
    21001 ttttgttgtg catctcatga gagaatccca aggaactcca tttgaatgga aagctggaga
    21061 ctcttatgta cagttgataa atatcgataa aaaagacttc ttaatcttga aaaatctctt
    21121 aggaaaattg aagtacagca gctatataaa agtagtgaac tattttgtca atacgatttt
    21181 ttcagaattt tcggagaaag aaaaaaatga aatcagtgag cagatacgaa aaaatatgtc
    21241 ctactgttcg ttttcatccg ctgattgcaa agaaaaaaac gaagaatatg ttgttcaatt
    21301 attaacgcaa atttattttt tctcccgtat tttcccatac gatacgactc atttgacgat
    21361 ccgtgcaact tactttgcac agcagatgca acaccgccag atacgtctat atgctggatt
    21421 tcgtgcagaa cttcaatttt attccactct actgaatcag aatctgattt attatttatc
    21481 tagctatatc ttttggatgc ttatcggtaa tcctgaaatt caccattata cttctgataa
    21541 aaaaatactg attatcagcg acttaagtct tcgacatagt caatatattg aagaatatat
    21601 cagtgatatt cttgctgtgc ataaaattca ctctgagaca actgctatta ctgaagatca
    21661 gctgtcgaaa tacaatcttc tcgagtatga tctgatcgtt acgaatcaac ccattttgaa
    21721 tgcccatgtt cctcatatat tgattgatga tagtgtgtca ttcgcaaatg aagaagaact
    21781 catccgctta ttcgagttat gaataatcaa aaaacagcta tgtataaacc accctgataa
    21841 aaatttcagg acaaatacac atagctgttt ctttttattg tattccttca gcaatccaac
    21901 gagatttata gcgaaagtct tttaacaact cttcttttgt ccaattattt gcaatgagat
    21961 ccgtcaaagt gttcattacg agggtcatgg aaaagtatag gaaactaact gcttcttccg
    22021 acgattgaat ggagaaatga ttgattctta ataattccag ccatttttcg ttcattggag
    22081 aatcagccga tagttctcta cgtttggttg tctgctgatc atttcgctgg gttaacaagt
    22141 ataacctttg ccaatagata ctttcgtgat ctaagtcgca gcaccactct aaatattttt
    22201 cgatccccaa aaagaaatgt tgtttgtttt cgctaatgaa ttttaagata tcttgatgga
    22261 ctatgtgaga actgcttttg atcgtataca aataagcatc ttccaaatct tgaaaatatt
    22321 tataaaaagc accgcgcgac atccccatct tgtcaacgat ttctgagact ttgacttggc
    22381 tgatgtgctg tgagcaaaat atctccagta ataggtcatc gattctctgt ttctttgcgt
    22441 tcgataaatt gaaaaaagtt tcttttggca tccacatctc tcctttatct ctgattatac
    22501 gtggatagcc cttcattgtc atgaaggatt cattccttct ttttctcttc ttctgtcaag
    22561 tacccatctt ccattcgata aacgcgatca ctccattgaa tcatacggac atcatgagta
    22621 accataatag tcgctttttg tttctcatga gcttctttaa ctagtaattt gacgacttca
    22681 tatgcatgat ctgtatccaa actagctgtt ggttcgtctg ctaagatcaa gcttggctca
    22741 ttatataaag cgcgagcaat tgccactctc tgtctttctc caccagaaag gtctttagga
    22801 taactatttt taagtgactg gatatctaaa gattgcagca aggaatcgat ttttgttgaa
    22861 tcttcttttt ttgaagtaac ttggtcaact agttcaaatt gttcctttac cgttaaaaaa
    22921 ggaatcaaat tagagctttg gagaataaat cctatttctt taaagcgtaa atctgctcgt
    22981 tttttttctg gaagctcagt tagtgagtga ccgttgatca ctatttctcc ggaagtcggt
    23041 gtttgcaatc ctcctgaaat ggtaaggaaa gtacttttac ctgaaccaga aggaccaatg
    23101 atgctgacga attctccttc ctttacataa aaatcaattg ctttcaaagc tgagaccttt
    23161 gtatgaccag aaccaaatgt tttggtaact tttttcatcg ttaatatttt tttcattttt
    23221 attaacctcc tattgctgta attgggtcta ctttggttat cgtacgtatc gaaaacaatc
    23281 cgcctagcat tgcgacaatg atcaaaatcc cactatatag caaccattgt gaccaaaaaa
    23341 cagcaaaagg catcgcgctt ggtaaaatca gactagtcaa ataagcaaac aaaagagcaa
    23401 gcagaacgcc taccacccca acaataaagg actgtgctac taaagattta gcgatgaaga
    23461 aatttttgat cccttgtgct ttcattactc caaaaatagc agtcttctga agtgtcatca
    23521 cgtacatgaa aataccgacc acggcagcaa ccactaagaa caaaaagtat atcatcgcat
    23581 ttaaggtcat attttgggca gaatagccag gaatactttc aatgaatgtc ccaatagtta
    23641 ataactgtag acccttcgat gctttttctt tcgaaatttt agctgaatcc tttattacga
    23701 tcccattgat cggctgttct tcttcagaag caaacggctg ttgtccatac ttcaaagcag
    23761 tccacgtatc tagggacgca tagatgacgg gagaaactgt gtaataagtt tcaggaaaga
    23821 taccgacaat cgtcaaagga tcgtcgtaac tgccaatttt gatcgtatct ccaattttat
    23881 agcctgaatc agccagattt tgagagatga tgatttcatt ctttgtttta aattcatgcc
    23941 ctttcgtgag ttttggtaat aaaaatgctt ctttcgttgt gccgaaaact gtaacatttt
    24001 ctttgttttt tcctttgatt gctccgctgt acaatccgat tggtgctttc tctcctcctt
    24061 caatgtattt cagatctcct cttgttaatt gtgaagctgc aaatacttga tttgcatcct
    24121 ccgacaaaac aatctcttgt gcgtcccaat catcgattgc ttttttaaac tcttctgaaa
    24181 gtccattcgc tagtcctgac aacatgaata cgacatacgc aatcaagaac ataataccga
    24241 cgatcaagcc atatcgtaat ttagaatagc gaatttcttt gatagctaga aacatttgtt
    24301 tttcctccct atgatagatt gatagtgaca aaatgtcact tgttgaatag tgtaactcta
    24361 aagtgacaat ttgtcacttg tgttgttttt ttcttatcaa taaattgtct aagacatctt
    24421 tcgataaaac cttaacatga gtcataaaaa atttatttaa gccttattta tgctttccat
    24481 gataaactaa agtgaccaaa tcaatgaggt gggaaaatga ttaaactatt agtagtagaa
    24541 gacgataaag tattatccaa taatataaaa gaaatactcg ctgcaatcgg cgagataaca
    24601 caagtttatg atggtagtga aggactgttc gagttggaaa gtggagttta tgatcttgca
    24661 atcttggatt tgatgatgcc ggaaatgaat ggatatgaag tgttagcaaa aatacggaaa
    24721 gaaaaaatac agactccagt attgattttg actgcaaaag atagtttgga agataagatt
    24781 gaaggatttg aacgaggtgc agatgattat ctaacaaaac ctttctatcg agaagaactg
    24841 atcatgcgcg tgaaagcatt attgaaacgc tcgctaggtc tctttgatga aaatgttctg
    24901 aagtataagg atatcagttg tgatttaaaa agcaatgaag tcttgtataa agaacaagtt
    24961 ttgcccatac aaggaaaaga gtttgaattg ctcgtttatt ttttacaaaa taagggaatc
    25021 attttgacaa aagagcagat atttgaccgg atctggggat ttgattctga tacgacgatt
    25081 acagttattg aagtttatat gagcaactta cgaaaacatc tgcgaagtac tggattggat
    25141 aaagaaatca aaaccttacg taatgtcggt tatatactgc aaagggtgtg atgcaatgaa
    25201 tggagatatt acacgaaagc aacgtagtcg cttttttgcg acaaatgtag cagcatttgc
    25261 ttttatcttt tttcttttgg gattcattac tttacagata ttaaaccaat ctgcatacag
    25321 agaaacagat ctgtcattag agaaaataaa ttttgattct cgaacagtac aattagagat
    25381 acaaagatat cagcaaggcg atcccttttt aaacccgact caaacggaac aaaacagaac
    25441 aaacatggaa cagccattaa tggaaccatc aaatgaccgt tttaacactc aagtgatctt
    25501 gtggtctaaa aatggtgaga tattgaataa agcagctatt ggtgggcgtt tcaaccaaat
    25561 caaagaatta aaaatggata cgaaaaatct gaataccatc caaaatattg aattaaatga
    25621 agacagtggc tcatctgccc tctctttccg ttcgattacg aaaaaagcgg agacgaatca
    25681 agatggcgtt gcctacatcc aaattttaga aaatacaaat caaatcaaga attcattagc
    25741 gacttttcgc acgattatga ttttgtgtat gattgttttt tggattctct ctattggtat
    25801 cagttattac ttgtctagtc taagcatgcg ccctattatt ctttcttgga gaagacaaaa
    25861 agaatttgtt gagaatgctt ctcatgaatt gcgcacaccg ttaacgatta ttcaaaatag
    25921 tttggaacat ctttttacca agcctaatca tactattatt gaagaatccg aaagcatcgc
    25981 tcaagcattg tcagagacga ggcgcctcac tggattgact tcggatttgc tgacaatcgc
    26041 tcggaatgat tctgatcagc agctgctttc aaagcaaatg atcaatacac aagaatatat
    26101 cgaacaactt gttaaaccat ttcaagagat ggccatcata gatggaaaag agtttattct
    26161 ccataacgat gcaacattag aagtaccaat tgatgaaaaa aaaatccatc aagttttggt
    26221 tattttgctg gataatgcat taaaatatac gaatccagaa gataagatca ttttggattc
    26281 atctgttaac cataaagaat ggctgattca cgtaaagaat actggccctt ctatcagtga
    26341 agaagatcaa gcaaggattt ttgatcgatt ttatcgggaa gatcaatctc gggcgaaaga
    26401 gactggcgga tacgggcttg gcttggcaat tgcaaaacag gtaatcgaac agcatcatgg
    26461 acagatttct gtatccaatt atcttccaca aggagtcgat tttaaagtcc aattgccgtt
    26521 gaagcctaaa aaataatgct tatccctaaa aaagggctgt gacaaaaatc ttgtcacagc
    26581 ccttttttgt tgcgaataaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat
    26641 ttctttaagc tgaccacttc tgtcacaacc tctttagctt acgcttacga ttttttattg
    26701 cggtcttcaa tgtgtacatc accgcttgtg tcaatatcca attcttctcg gctaacatct
    26761 tctgtcactt tttcaacatc ttcatgttct tctttatgaa tcccaacttc ttcacgaata
    26821 actgtatgtt tgcttacatc tacttgctct tctttgattg ggatagtaat cgtgtcatct
    26881 tccaattttt cgtctgttcc ctgtgaagat tgatctgtta ctggtttgcg ttcgatgacg
    26941 atttcttcac gtttgaccgg tacttctacc gtttcagtat cgttgaccac atgtttgtgg
    27001 atgtcgactt ctccagttgt cacatcatgt gtatttacat ctaatttttc ttcttttagt
    27061 tgaattgtat catcatctag atttggtttg cgatcgatgt cttcagtctt gcttgtaccc
    27121 attgtatcag tctcgccagg tacgtccatc ggatcagttc ctggtgtcat attcaatgga
    27181 tccgttgtat tttgaaagcc tgtcgtgcca cctacgtcca ttggatctgt tccagtgcca
    27241 gtttctaatc cagtccccat tgtgcttgct gggctaacag tatcttgtgt atccattgta
    27301 tcttctgttg tttctggctg acgatagcct tttactgtga tgacaaattt tccatcagaa
    27361 atatctcctc tatattgata taaaggatca ttttctggtg tagccgtctc agaatcatag
    27421 gtatcaaaag aaaaagtatc ttttattttt tcccataaag aacggtcttc ttcaccatcc
    27481 atccgatttt catctgtttc aacatctata ccagacaatc cgtcaaatcc tagtctagat
    27541 gctgcttcat cagtagtata aataacgata tcatctcgtt catacccttc gttacgcaac
    27601 cgttcaatta ctgataatgc ttcttccctc gtactatagc ttccgatgac cgttaaattt
    27661 ttctcttcca tgtaaattcc tcctagcaaa tagatttatg attggtatat tgttacttat
    27721 aaatttattc tataggagac gattgttttc tacaaatatt tagattggaa tttgattaaa
    27781 acaatttaat tgaaatttga aaacatttat gttaatgaaa gtcatattct aataaaatgt
    27841 catttgtttc tatatgaatt tttatgaaaa atttcatata atttccactc gttgtgatgg
    27901 tttaatttat ccaatccatc aataagcaaa caacttatat aaaaaaggat taattctcaa
    27961 tataattgct cctaccatag aataaatctt catgtaaagt aatgaagtac caactatttc
    28021 taatacttct ttaagaatta tcccaacaat atcgttagac aaaccattat tgaataaatg
    28081 gagagccaat cgattctgaa aaaatgtttt ctaatttttc ggaaataaaa gaaagagcct
    28141 cctgccatta gaacgaacca aatccatttc attgctcatg aagtaaccac atagctaaaa
    28201 agaggataaa tcaattaaaa gatcaaaaga atgactgcac taataaaata actaaaaatg
    28261 tatcttctta cttcatatct tttagctagt acttagttaa aaatttgctt cctaagatca
    28321 agacaaactg tctatcttca tttgatgaaa tgcggtaaaa tatagaaaat gaacactatt
    28381 ggagggaatt tgtaatggac agcaaaacta gaaaacaact gcaagaaaag ttaaagcaac
    28441 gtacacactt gctcccttcc gtctttcaac agtttttaga agaaaatcgt tcttcccttt
    28501 cattaggcag tcgctatgaa tacgcaaaag attttcatct gtttactgat tatttgagaa
    28561 aagtattttc tgaagaagtg gatgatcagc tgattatcca tttagaaaaa caagattatc
    28621 agaatttctt agctaaaatc tatcgacata ctcgttcgtt ccaaactaca gcgaaccaag
    28681 aaacggagca attgtttgaa aatagctatt acgggatttc aaggaagcaa tattcattaa
    28741 atcatttttt acgatttttg tatcaaaaag gactgattga agaagagatt tctcttttgg
    28801 gtacaagttt accggaaaca accaaagcag ctccgcgata tatcgatgca gaacttttca
    28861 aagattttga gcatttgttt atcccgattg aaggctttca aacaagcaga gaagctagct
    28921 ttgaagaaaa gaatagaccg aggaaccttg caattactgc cattcttctg tattgcggat
    28981 taaagatcca tgaagtagta gaacttaaac gtaaagatgt atctttgacc aagcagattc
    29041 ttaatctgaa tagaaaagaa aacagactga ataaagtacc aataccaaaa gaagcgatgc
    29101 catttctgga gaactatttt aagttaacag cacaaaatac ggacgaaaat tcttttgttt
    29161 ttcgttcctc acatgatcag caaatttctc ctcggactat tcgccaaatt ttgagcaaaa
    29221 ttacagtata taaaaacgta tcgccagaac tatgcagaaa aacatatcgt tattatgcag
    29281 aactatattt agatgatgct gacgcggtaa attatttatg tggtaatcga tacctctcat
    29341 cccattcttt tcaagatatc tgggaaaaga tggttgattt ttcttatcac gaattagcgg
    29401 cattcgttcg ggtttcttcc atataaaaca aatgtatgat ctttttttca caataaacta
    29461 tgttattata taaaggatta tgttacgatt atcttgaatt tgtatattac ttaacaaaag
    29521 ataatggagt gtgaatgatg gagaccataa cgtattcccc tttgaactta tttacgaatt
    29581 ttcaagaagc tgcagctaac ttttccgaga ccccgattat ctttgatgag ccactttcag
    29641 ctttcccaga attaggactt gaaacaactt acaaaaaatg ctgcgaaagt gtgttgaaac
    29701 gggcttatca attggctcat cttggagtga aatccggaga caaagttatt atttataaga
    29761 gttccgcttt tgacacatac cttctagctg tttctgtgtc ttaccttggt gcagtgcctg
    29821 tcatgacttc ttatcatcta ccaacaacaa caatggaagt atttatcgat cgtttagaag
    29881 accctttcat tttatttgat gatgagacaa aaaagcgagt gagagaaatt tcaaatggaa
    29941 caaaatcaaa acagattcct
//
LOCUS       DAYYCQ010000027        29201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000027 DAYYCQ010000000
VERSION     DAYYCQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.027"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHAYF_001216"
     CDS             complement(99..1229)
                     /locus_tag="ACHAYF_001216"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8578668.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHAYF_001217"
     CDS             complement(1878..3080)
                     /locus_tag="ACHAYF_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8578669.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHAYF_001218"
     CDS             complement(3140..3343)
                     /locus_tag="ACHAYF_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8578670.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHAYF_001219"
     CDS             complement(3485..4552)
                     /locus_tag="ACHAYF_001219"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8578671.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHAYF_001220"
     CDS             complement(4610..4717)
                     /locus_tag="ACHAYF_001220"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578672.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHAYF_001221"
     CDS             4880..5692
                     /locus_tag="ACHAYF_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578673.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHAYF_001222"
     CDS             complement(6066..6257)
                     /locus_tag="ACHAYF_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578674.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACHAYF_001223"
     CDS             complement(6502..6732)
                     /locus_tag="ACHAYF_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578675.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACHAYF_001224"
     CDS             complement(6716..6913)
                     /locus_tag="ACHAYF_001224"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8578676.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="ACHAYF_001225"
     CDS             complement(7059..7937)
                     /locus_tag="ACHAYF_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8578677.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="ACHAYF_001226"
     CDS             complement(7976..9094)
                     /locus_tag="ACHAYF_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8578678.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="ACHAYF_001227"
     CDS             complement(9091..11190)
                     /locus_tag="ACHAYF_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8578679.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="ACHAYF_001228"
     CDS             complement(11241..12062)
                     /locus_tag="ACHAYF_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8578680.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="ACHAYF_001229"
     CDS             complement(12059..12868)
                     /locus_tag="ACHAYF_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8578681.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="ACHAYF_001230"
     CDS             complement(12889..13383)
                     /locus_tag="ACHAYF_001230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8578682.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13895)
                     /locus_tag="ACHAYF_001231"
     CDS             complement(13395..13895)
                     /locus_tag="ACHAYF_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8578683.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13960..16656)
                     /locus_tag="ACHAYF_001232"
     CDS             complement(13960..16656)
                     /locus_tag="ACHAYF_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8578684.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="ACHAYF_001233"
     CDS             complement(17084..18157)
                     /locus_tag="ACHAYF_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8578685.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="ACHAYF_001234"
     CDS             complement(18154..18981)
                     /locus_tag="ACHAYF_001234"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578686.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="ACHAYF_001235"
     CDS             complement(18982..19788)
                     /locus_tag="ACHAYF_001235"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578687.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="ACHAYF_001236"
     CDS             complement(19800..20885)
                     /locus_tag="ACHAYF_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578688.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="ACHAYF_001237"
     CDS             complement(20902..21444)
                     /locus_tag="ACHAYF_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8578689.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="ACHAYF_001238"
     CDS             21702..22691
                     /locus_tag="ACHAYF_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8578690.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="ACHAYF_001239"
     CDS             22688..23425
                     /locus_tag="ACHAYF_001239"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8578691.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="ACHAYF_001240"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="ACHAYF_001240"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8578692.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="ACHAYF_001241"
     CDS             23989..24621
                     /locus_tag="ACHAYF_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8578693.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="ACHAYF_001242"
     CDS             24706..25353
                     /locus_tag="ACHAYF_001242"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8578694.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="ACHAYF_001243"
     CDS             25358..26341
                     /locus_tag="ACHAYF_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8578695.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="ACHAYF_001244"
     CDS             complement(26744..28258)
                     /locus_tag="ACHAYF_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8578696.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..>29201
                     /locus_tag="ACHAYF_001245"
     CDS             28396..>29201
                     /locus_tag="ACHAYF_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578697.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         8830 a         6175 c         4943 g         9253 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatatag gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat a
//
LOCUS       DAYYCQ010000028        29097 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000028 DAYYCQ010000000
VERSION     DAYYCQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.028"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHAYF_001246"
     CDS             complement(<1..1031)
                     /locus_tag="ACHAYF_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8578698.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHAYF_001247"
     CDS             complement(1113..1802)
                     /locus_tag="ACHAYF_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8578699.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHAYF_001248"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHAYF_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8578700.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHAYF_001249"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHAYF_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8578701.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHAYF_001250"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHAYF_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8578702.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHAYF_001251"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHAYF_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8578703.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHAYF_001252"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHAYF_001252"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8578704.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHAYF_001253"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHAYF_001253"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8578705.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHAYF_001254"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHAYF_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHAYF_001255"
     CDS             complement(7636..8523)
                     /locus_tag="ACHAYF_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8578706.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHAYF_001256"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHAYF_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8578707.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHAYF_001257"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHAYF_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8578708.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHAYF_001258"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHAYF_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8578709.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHAYF_001259"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHAYF_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8578710.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHAYF_001260"
     CDS             complement(10454..10957)
                     /locus_tag="ACHAYF_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8578711.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHAYF_001261"
     CDS             11554..12519
                     /locus_tag="ACHAYF_001261"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8578712.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHAYF_001262"
     CDS             complement(12618..13790)
                     /locus_tag="ACHAYF_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578713.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHAYF_001263"
     CDS             complement(13777..14922)
                     /locus_tag="ACHAYF_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578714.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHAYF_001264"
     CDS             complement(14936..15868)
                     /locus_tag="ACHAYF_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578715.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHAYF_001265"
     CDS             15985..17064
                     /locus_tag="ACHAYF_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8578716.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHAYF_001266"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHAYF_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHAYF_001267"
     CDS             complement(17789..18016)
                     /locus_tag="ACHAYF_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8578717.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHAYF_001268"
     CDS             complement(18119..20707)
                     /locus_tag="ACHAYF_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8578718.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHAYF_001269"
     CDS             complement(20727..21386)
                     /locus_tag="ACHAYF_001269"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8578719.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHAYF_001270"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHAYF_001270"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8578720.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHAYF_001271"
     CDS             complement(22159..23025)
                     /locus_tag="ACHAYF_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8578721.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHAYF_001272"
     CDS             23134..23775
                     /locus_tag="ACHAYF_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8578722.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHAYF_001273"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHAYF_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8578723.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHAYF_001274"
     CDS             complement(25216..26115)
                     /locus_tag="ACHAYF_001274"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8578724.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHAYF_001275"
     CDS             complement(26117..26914)
                     /locus_tag="ACHAYF_001275"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8578725.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHAYF_001276"
     CDS             complement(26892..27596)
                     /locus_tag="ACHAYF_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8578726.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHAYF_001277"
     CDS             27727..28302
                     /locus_tag="ACHAYF_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8578727.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHAYF_001278"
     CDS             28420..29097
                     /locus_tag="ACHAYF_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8578728.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8560 a         6167 c         5232 g         9138 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaa
//
LOCUS       DAYYCQ010000029        29096 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000029 DAYYCQ010000000
VERSION     DAYYCQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.029"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..1063
                     /locus_tag="ACHAYF_001279"
     CDS             278..1063
                     /locus_tag="ACHAYF_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8578729.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1076..1927
                     /locus_tag="ACHAYF_001280"
     CDS             1076..1927
                     /locus_tag="ACHAYF_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8578730.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2051..2503)
                     /locus_tag="ACHAYF_001281"
     CDS             complement(2051..2503)
                     /locus_tag="ACHAYF_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578731.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(2968..3336)
                     /locus_tag="ACHAYF_001282"
     CDS             complement(2968..3336)
                     /locus_tag="ACHAYF_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8578732.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3486..3968
                     /locus_tag="ACHAYF_001283"
     CDS             3486..3968
                     /locus_tag="ACHAYF_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8578733.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4005..5015
                     /locus_tag="ACHAYF_001284"
     CDS             4005..5015
                     /locus_tag="ACHAYF_001284"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8578734.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5130..5843)
                     /locus_tag="ACHAYF_001285"
     CDS             complement(5130..5843)
                     /locus_tag="ACHAYF_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578735.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(6016..6623)
                     /locus_tag="ACHAYF_001286"
                     /pseudo
     CDS             complement(6016..6623)
                     /locus_tag="ACHAYF_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            7107..7334
                     /locus_tag="ACHAYF_001287"
     CDS             7107..7334
                     /locus_tag="ACHAYF_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8578736.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            7350..8516
                     /gene="hemW"
                     /locus_tag="ACHAYF_001288"
     CDS             7350..8516
                     /gene="hemW"
                     /locus_tag="ACHAYF_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8578737.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            8750..9793
                     /gene="hrcA"
                     /locus_tag="ACHAYF_001289"
     CDS             8750..9793
                     /gene="hrcA"
                     /locus_tag="ACHAYF_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8578738.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            9887..10450
                     /gene="grpE"
                     /locus_tag="ACHAYF_001290"
     CDS             9887..10450
                     /gene="grpE"
                     /locus_tag="ACHAYF_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8578739.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            10501..12330
                     /gene="dnaK"
                     /locus_tag="ACHAYF_001291"
     CDS             10501..12330
                     /gene="dnaK"
                     /locus_tag="ACHAYF_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8578740.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            12481..13647
                     /gene="dnaJ"
                     /locus_tag="ACHAYF_001292"
     CDS             12481..13647
                     /gene="dnaJ"
                     /locus_tag="ACHAYF_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8578741.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(13729..14358)
                     /locus_tag="ACHAYF_001293"
     CDS             complement(13729..14358)
                     /locus_tag="ACHAYF_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578742.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            14370..14543
                     /locus_tag="ACHAYF_001294"
     CDS             14370..14543
                     /locus_tag="ACHAYF_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578743.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(14588..15325)
                     /locus_tag="ACHAYF_001295"
     CDS             complement(14588..15325)
                     /locus_tag="ACHAYF_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8578744.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(15327..16829)
                     /locus_tag="ACHAYF_001296"
     CDS             complement(15327..16829)
                     /locus_tag="ACHAYF_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8578745.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            17088..19082
                     /gene="uvrB"
                     /locus_tag="ACHAYF_001297"
     CDS             17088..19082
                     /gene="uvrB"
                     /locus_tag="ACHAYF_001297"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8578746.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            19092..21908
                     /gene="uvrA"
                     /locus_tag="ACHAYF_001298"
     CDS             19092..21908
                     /gene="uvrA"
                     /locus_tag="ACHAYF_001298"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8578747.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            22171..23436
                     /locus_tag="ACHAYF_001299"
     CDS             22171..23436
                     /locus_tag="ACHAYF_001299"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8578748.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            23440..24171
                     /locus_tag="ACHAYF_001300"
     CDS             23440..24171
                     /locus_tag="ACHAYF_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8578749.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            24306..25190
                     /gene="rapZ"
                     /locus_tag="ACHAYF_001301"
     CDS             24306..25190
                     /gene="rapZ"
                     /locus_tag="ACHAYF_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8578750.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            25187..26185
                     /gene="yvcK"
                     /locus_tag="ACHAYF_001302"
     CDS             25187..26185
                     /gene="yvcK"
                     /locus_tag="ACHAYF_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8578751.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            26219..27154
                     /gene="whiA"
                     /locus_tag="ACHAYF_001303"
     CDS             26219..27154
                     /gene="whiA"
                     /locus_tag="ACHAYF_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8578752.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            27697..28347
                     /locus_tag="ACHAYF_001304"
     CDS             27697..28347
                     /locus_tag="ACHAYF_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578753.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            28534..>29096
                     /locus_tag="ACHAYF_001305"
     CDS             28534..>29096
                     /locus_tag="ACHAYF_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFM8578754.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         9565 a         4897 c         6176 g         8458 t
ORIGIN      
        1 aaggggctgt gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa
       61 gtagcttctt cggaaataag ccgaaatttc ttgaagctga ccacttctgt cacaacctct
      121 ttctttatag aaataaaagg acactagcaa ttcatgaaaa cgattgctag agcgtatcat
      181 ttgctttaga ctatatatct tggtgtacac tcactttaag gcggaaataa aagttatccg
      241 taataacagg tttaggtatg aaaaaggagt gggtactatg tacaaggaat tagaaaacaa
      301 agtcgcagta gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg
      361 gaaagaaaaa atgaatgtag ttgtcaacta taactcggat aaagaaggag cagaaaaagc
      421 agtcaaagca atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc
      481 tgaagaagga atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt
      541 ttgggttaat aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga
      601 ttgggaaaga gtgttaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt
      661 aaattacttt acagagaata ataaaaaagg aaatatcatc aatatgtctt ctgttcatga
      721 acagatccca tggccaacgt ttgctcatta cgctgcttca aaaggcgggg taaaattatt
      781 taccgaaacg gtagcaatgg agtatgcacc gaagggaatc agaatcaata gtataggccc
      841 tggtgctatc cgtacaccta ttaatgcggc aaaatttgat gatcctgaac aaaaagaact
      901 cttggaaagt atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc
      961 tgcatggttg gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg
     1021 tgggatgact ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg
     1081 tttattgatt gcattgatcc cggcagttgg ctgggggatc cagccactga ttttgaaaaa
     1141 gattggtggc agaccaacaa atgagatatt aggaactggt attggagcgg tgcttgtcgg
     1201 cttactcgtt caattattcg cttctccagc tggtatttca gtgactacat ttttgattag
     1261 tttactttct ggtgcatttt gggttattgg acaaacagga cagtatacat ctttcaactt
     1321 gattggtgtt tctaaaacga tgccaatttc gactgcatta cagttagtcg gaacctcgtt
     1381 gattggtgtc ttcgcttttg gagaatggtc tggaagtatg gggaaaatca ttggagcagc
     1441 agcaatcatt cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa
     1501 aaaaggcggt atcacaaaag gaattagtct attagcatca acaagtattg gttattgggt
     1561 atacagtgca ttgcctaaac tagttgatgc aagcggactt tccattttct ttccgcaaat
     1621 gatcggtgtc tttttagggg cagtgattta tgtgattttc cgtaaaccaa ccgcattttc
     1681 tgaagacaag agttggaaag caacgatcgt tggagccatc tttagtgtgt cagcattggc
     1741 ttatattttt tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa
     1801 cgtagttatc tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga
     1861 aaaaaaatta acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt
     1921 tatttgagga taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac
     1981 agtaagtact tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct
     2041 ttttaattgt ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc
     2101 attgactaaa gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt
     2161 ctttataaag atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt
     2221 actggactaa ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga
     2281 aacgcatcgt gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg
     2341 cttgatctcc gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa
     2401 cctttgttcc ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac
     2461 ctaattgttc ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt
     2521 gaatggttct actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt
     2581 taattaaggt cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaagaaa
     2641 ttcgttctct cctaaagtga tattatattt aaatatacct gaaaataatg ggcagtatta
     2701 tgaaagatca ctgatatttt ctcgtatttt ttccttattc aaaagttttg caggaaaatt
     2761 gctgaaaaaa taattttact aagtagaaaa ttcaaagcca ttcaatcagt gatttttttc
     2821 agcacaagaa agactcctta taaaaatagc agatactttg tcaattaagt gtttaaccag
     2881 ggatgataaa aaggatagga ttgaacaaaa cctgtataag gtgatgaata gtttcctcgc
     2941 cagtaaaagg tttattcgta taaagcttta tcgccaaaat ttatcatgac cttttttatc
     3001 ttcaatatta aatgaaatca attgttcaat caattcccag ttgattacac ttttccaagg
     3061 aactttgatg acgttatccg tatatacgta gcctgcttct tggatatttt tttcaagggc
     3121 acgaatcgct gctttttccg gtgcgataga aaaatgattt ttagcaaaac taaatgcaat
     3181 aataaaggta ccatggtcag taaacatagg ttggttccat tttacagttg tgtctagctg
     3241 gggatacgtt tctgttatct tagcaaaaag gtctttgatc ttttgttgat gttctttttc
     3301 tttgatttga ttgatgatcg gctgaaattt ttccattcta aaacgcctcc attaagtggt
     3361 ttgatttatt ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa acacaagtaa
     3421 tgttgtaacc gctatattta tatgataaga tgtctcaaat aataaaatag aaaagagaca
     3481 taaaaatgat tgacttggca aagaaattct tacacgaaaa tatatatttt gagttgtatc
     3541 atcatcgagc catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta
     3601 caggtacaga gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc
     3661 tgacttttac tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc
     3721 tttctattgt ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt
     3781 ctccgtttgg ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg
     3841 aaaaacttgt ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact
     3901 taaacaaaat catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa
     3961 acaattaacc aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat
     4021 tattagaaag aaatgcaacg attggggtta ctgcaccatc ctcaggcgta tcagaggaac
     4081 accattcgtt actcgaatca gctttaaaga gaatggaaga aaaatctttc caagtagtta
     4141 ctggaaagac gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag
     4201 atgaattcaa tcaaatgatg caagatgaaa atatccaaat gatctttccg acttggggag
     4261 gagaattatt gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga
     4321 tagtaggtta ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag
     4381 ccactgcaca tggaactaac cttattgatt taagaggaga gtacttggat gaaacgacag
     4441 caatgtggga aagtgtactg aaaacaagag aaggagaatc gattctacag caatcttctg
     4501 aaaagtatca aaaagtgtgg ccccatgagc attcatcatc ttctgttttc catctcactg
     4561 aaccaacaaa ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag
     4621 gtggttgtat tgatacgatc aggcatttaa tcggtactcc ttatggagat gtccaatctt
     4681 ttaaaagaaa gtataatgat ggaaagccga tcttgtggtt tctagaaaac tgtgagctat
     4741 cgtcagttga tttaagaaga tcacttgttc agatgaaact ggcaggctgg tttgatggca
     4801 gctcggggat catgttcggt agaagtgcgg cagaggattt gttagattat aaaatgaagg
     4861 atgtatacga agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg
     4921 gtcatgtacc gccacaactg acattgatta atggtgccta tgcagaagta gcggtagaag
     4981 atagaaaagc gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagtct
     5041 atcaaagacc aataaatcta aatagaaaag aaagtgaaag aagctgacaa aatgctgtca
     5101 gcttcttttt gtataacata ttagaaagtc taattatcta taactgtcac atcgattaac
     5161 tgtttcacgt cattgggatt tcctgtagga atagctacta cagatgcatt atcaatgatt
     5221 tcttgagttg tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa
     5281 tgtgaaggaa ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact
     5341 ccctggtaac tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct
     5401 gcatcataag gggcgatagg tgttcctgca ggataatgaa taacatgtaa ttcgaaatca
     5461 cccgatgtat ctctaaaagt attacctcta atagctagcc aaacacgtgc gtactcaatt
     5521 tgtagatcag aatatcctgc taacggatct attgactgag aagactgtga ggaggttgtg
     5581 tctattgttg aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg
     5641 ggctctgaga ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga
     5701 gtacttgatt tttcagctga actggtttgt ctgattgaac ttttgtctgt ggtcgcagat
     5761 ggatcactat tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat
     5821 aaaataaaac ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc
     5881 ataatcctga atttttgaat ccgtaaaacc ttgtctatga atataaatag agtgtatctt
     5941 ctttgataaa tgtcagaaaa agtgtgacaa ataatcatca gtaaaattac ttgtcacact
     6001 attgataagg agtatttaat tgaagagaat gattaggaga aaggatctcc gtacttttta
     6061 tagtaatcat agaatccaag tttgtataga cgaatcgttt tttctcttat ttcttctgtt
     6121 tcaaaatgga ctgcttgaag aatcaagtct gtgaattcta taggagctgt tccattcgca
     6181 gtaatcaaat tacgatcttt cacagcttgt tgttctataa aatcttcttt attctgataa
     6241 ttttccgatt ctaaccaagg aaattgcgca tttccggtgt gtttgtaacc ttgcaataat
     6301 ccgctctgag ctaaataatc aactgcccca caaatagcag ctactggtac gtctgttttt
     6361 aacaatttat cgattatttg atataaatga tgatttttta tgtcccatga attgccaccg
     6421 atcataataa aaaggtcaac tttttcaggt atttgttcga ttaagtaatc gatttttgtt
     6481 tttaatcctc caatggaagt gacctctgat tggatagaag cagttttgtt ttgccatttc
     6541 tcattttggt ttagtgtgct agtaatataa gcaccttccc aatcggcata ttcctctaat
     6601 agaaacgtaa tcgcagtttt cattttcaag ctctccttta ctatctataa caaggacaac
     6661 agttttaacc tacagacatt cattaaaatt gggtagcgac agcgaaaaca acaattctta
     6721 agttttagac agagtataca gatagtcaac ttaattaaat acgaactaaa taaaaatgac
     6781 aggtaatcat ttttgtttac cagcattata acattttgca tacatggatg acaaaagaac
     6841 caattcccac ttgcttgagt acaatattaa aagaaactgg aaggcaaaaa gtaattatag
     6901 aaacgaaaaa taaatcatat cgggcactca gacatgaaaa caaccctaga gatatataac
     6961 taggtaacca gtttaatgaa agaaaaaatc atccaagaag tcgattcttg gattttttag
     7021 tggatttgtt cgccaaatac gtttagtatg cgtatatgag aggttgtaag caaagcgttg
     7081 tggtctgagc acttaaggag gaatctgtga aaatagcttt tcatattttt gcagattttg
     7141 acttaatgcc gaaggtcagc tttgcgatta ccgtttatcg agaggctgta aaagcaccgt
     7201 gtagctccga tgacaataag caagcatttt tagagctagg tttccttgat tctaaatatg
     7261 cggcttattt caatgagttg gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg
     7321 acttttcgac gtaaggagaa agaatttcaa tgatagaaac aataaatccc gcagaaaaca
     7381 aggtttctgc gtacattcat atcccatttt gcgagcatat ctgctattac tgtgatttta
     7441 ataaagtttt tttagaaggg cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa
     7501 tgaaaataat gctagaaagt catccgacag cgaatttaga aacactttac gtagggggcg
     7561 ggacaccaac ttctttatct gctaaacaac ttgatcgact gttatcaggt gctagagaga
     7621 tcctgccttt tcaaaatggt aatgagttta ctgtagaagc gaatccaggt gatttgaccc
     7681 gtgaaaaact tcaagtaatg aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga
     7741 catttgataa tcgcttattg aaaaaaattg gacgtaaaca caccgcagaa gatgtgtatc
     7801 aaactatgca atttttagaa gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg
     7861 cattaccggg acaaacacta gaaggatatc tggatacttt ggaaaaagct ttggctttgg
     7921 atctgcctca ctattctttg tactcattga tcctcgaaaa caaaacaatg ttcatgaact
     7981 gggtacgaca aggaagactc gaactgcctg atcaggaaac ggaaaccaga atgtttgaag
     8041 agacgattga agcaatggaa aagcatggac gtcaccaata tgaaataagt aactttgggc
     8101 tctccggaca tgaatctcaa cataacctca tgtactggaa caatgcccac tattttgggt
     8161 ttggagctgg tgctagcggt tatcttggag atattcgtta ccgcaacaaa ggaccaatcc
     8221 aacactattt acgcccatta cgagcaaatg aacgcccggt attggaagaa gaaaaattga
     8281 cacgaaaaaa tcaaatagaa gaagaaatgt tcttaggact aagaaaaaaa acagggatta
     8341 ctaaaaaaca tttctatgat cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc
     8401 taagggaatt agagcaagaa gggttacttg ttaatgaaga agaccgtgtt tatttgacac
     8461 caagagggac ctttttgggg aacaatgtgt ttgagcgctt tttgtttgaa aaatagttta
     8521 aagaatattt gttttttatc tgaatttaaa taaaaaaagt tcaaaaatac aataaaggac
     8581 tattgaaacg ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt
     8641 tagagtgcta aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat
     8701 aagtggatta gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa
     8761 gcgacaaaac gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt
     8821 tggttccaaa acattgatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga
     8881 tatgaaagca ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat
     8941 tccatctgct ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc
     9001 agaagatgat ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga
     9061 gattattaag caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt
     9121 gggacctgaa ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg
     9181 acagattatt gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct
     9241 tcccgaatat atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct
     9301 agtcggagat cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct
     9361 tcataaatat ttccaaacga cagaagggat gtctcagtta tttgacaccg ttcttggtca
     9421 tgtttttgaa gataaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg
     9481 tcaagatctt gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac
     9541 acaaatgatc actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa
     9601 tgatttattg cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag
     9661 aggtacaata gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt
     9721 cgatgtttat cgaagagaac tagcagctaa acttgctgat tactaccgtt cgcttgactt
     9781 gtcaaactcg taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt
     9841 aagagcaatc aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca
     9901 agaagaacta gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc
     9961 tgcagaagat tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa
    10021 agctgagtta gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat
    10081 ggcaaatcga ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc
    10141 caaaaaatta cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga
    10201 tgatcaaggg gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc
    10261 actggaagaa gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct
    10321 tcatcaagca gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga
    10381 agtactacaa gaaggctaca aattacatga tcgcgtgtta agaccaacca tggtcattgt
    10441 tgcacaataa ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt
    10501 atgagtaaaa ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa
    10561 ggaaacgaag caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta
    10621 tcattcaaaa atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac
    10681 ccaaatacga tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta
    10741 gaaggtaaat catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga
    10801 ttcgcagaag actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac
    10861 tttaatgatg cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta
    10921 gaacgtatcg tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat
    10981 cgcgacgaaa aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt
    11041 gaactaggag acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg
    11101 gacgactttg ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc
    11161 attgacttgt ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg
    11221 aaaaaagatt tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga
    11281 gaatctggac cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca
    11341 gctgacttag tagaacgtac aaaagtacca gtacgtcaag cgctaaaaga tgcaggttta
    11401 tcacaatcag aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt
    11461 gtagaagcag tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa
    11521 gtagttgcta tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt
    11581 gttttacttg acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact
    11641 aaattgattg accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg
    11701 gcagataatc aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct
    11761 gacaacaaaa ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt
    11821 ccacaaatcg aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa
    11881 gatctaggta cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac
    11941 gaagaaatcg aacgtatggt aaaagatgca gaagcaaatg cagaagcaga taaagctcgt
    12001 aaagaggaag ttgacctacg taatgatgta gatgcattgc tattctctgt ggacaaaaca
    12061 ttgaaagaat tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgc
    12121 gatgaattga aagccgctgt tgaagcaaac aacatcgaag aaatgaaaac taaacgcgat
    12181 gcattgaacg aaatcgttca aaacttgact gttaaactat acgaacaagc ggcacaacaa
    12241 caagctcaag aaaacccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat
    12301 tttgaagaag tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa
    12361 agttgctact ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa
    12421 caaaatatga tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct
    12481 atggcaacaa aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat
    12541 gaaataaaga aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa
    12601 cctgatgcgg aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg
    12661 caaaaacgcg ctgcatatga ccaatacggg catgcaggca cagatccaaa ttacggtgct
    12721 ggtggagctg gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt
    12781 tttgaagata tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg
    12841 cctcgtcaag gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt
    12901 ggtgtagaaa aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtgggaac
    12961 ggtgcaaaac caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatt
    13021 aatgtagaac gccaaacacc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt
    13081 catgggacag ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag
    13141 aagaaagcac attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg
    13201 cgtttagctg gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg
    13261 gtcttccgtg tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa
    13321 ttgcctttaa gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat
    13381 ggagatgtga aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt
    13441 ggaaaaggcg ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa
    13501 ctgatcacac cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa
    13561 gctggcggca tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat
    13621 gcttttggcg ggaaaaagcg gaagtagttt ttctagtaaa aacgctaagt cattaataat
    13681 taaaaaccag acgaacaatc atatgttcgc ctggtttttt tgtagttgtc atgtttgtac
    13741 caacaaaaaa gccagcttta gaatatgata atcgtcatag tttctagggg tataaccagt
    13801 gagagaatgg aacttgttca atcgatattg caaggtgttt ttatgaataa ataattcttc
    13861 tgagcaattt ttgatgcttc cgttattttt actgaagcat gccaaagtct tcttgaacaa
    13921 atgtacttct tcttctgtca ggcttttcaa aatacgtttt acatataatg ttcgttgatc
    13981 tgacgggatt gctggtaaaa cgatcccgag gtctaaatct tcaaaaaagc ggatctcgtt
    14041 cacagattga tggaaagcta tccattcgca aacttgtttg gcatgaagga aagattgcgg
    14101 tgtctcttgc tctgtataac aaagcgaccc aattccaaag tttagctgta actgatacat
    14161 tttattaaac tttgtctgga ttttatcaag caatgatagc aatgaggctc tggaaatccg
    14221 attgactagg ataatgattt gttgtctatg tacagtgagc aaagcctgtc cgtaataaga
    14281 gaagaccctt tcgagcaact ccaataaagg attgatccgt tcttgacaca attgctgatt
    14341 gtcagaaagt tgtcccacaa ctacataata tggagaagaa aaatcaagct caattaagtg
    14401 agggtggtat cgactatgta cttgatatca tcaatgaacc gatggtactt tcttatgcga
    14461 tggaacaggt agagcgattg cttgtttgtg caggaaaaca agcgatgcgg ctgatacgat
    14521 taggtgagaa aataagaaga taaaaataca cggtcccttc atggcgaccg tgcatttttg
    14581 gtttttatta aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat
    14641 tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt tccgtcgtca atgaacatga
    14701 cgcgattggc cacttcttta gcgaacccca tttcgtgcgt aacgataacc atcgtcatac
    14761 cttgttctgc taactttttc atgacgttca agacatcacc gaccatttca ggatcgagtg
    14821 cagaagtagg ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag
    14881 ccacacgctg tttttgtcca ccagaaagca tttcaggata catatctttt ttatcttcta
    14941 gaccaactgt ctgcagtaat ttgattgctt ttttctctgc ttcttctcta gattctttct
    15001 tcaggtccat cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa
    15061 agtgttggaa taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca
    15121 tcaagtgggc gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc
    15181 aacgcaagaa tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt
    15241 cgttgatgga tacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat
    15301 gctcaactaa gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat
    15361 tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta
    15421 ctttgtgttg tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt
    15481 gcggaaatga tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc
    15541 atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct
    15601 aaacttctgg aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc
    15661 tctgcgatat aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga
    15721 atcgtaaatc caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt
    15781 ggaattcctc ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt
    15841 gctacactga ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa
    15901 gttaggagaa ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt
    15961 ccaagaaatg ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct
    16021 acatattctt taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca
    16081 ttgaacatat taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg
    16141 ccagattctt ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt
    16201 accggataat catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga
    16261 tcagcagcat caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat
    16321 tcagcacttt cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac
    16381 ttgatcgaag tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca
    16441 gaaaaatcat agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct
    16501 gcttgtcctg attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg
    16561 ttaaatcctt gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg
    16621 ccctttgcgt tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg
    16681 tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg
    16741 ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa gtcctgtcat taagaaaaca
    16801 aatgagtgaa ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt
    16861 attataggcg aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg
    16921 cgagtttcta acattttatg ttataaaaca taacatgtat aataagataa aaaagtggcg
    16981 aaagtatgtt cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt
    17041 ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag
    17101 atacctcccg acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag
    17161 cgattaacca attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg
    17221 cgactggaac agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa
    17281 cactgattat tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat
    17341 ttttccctaa caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag
    17401 cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg
    17461 acaaattacg acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg
    17521 catccgtttc atgtattttt ggattaggct cgccttttga gtaccaaaaa caagtagttt
    17581 cgattcgaca gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc
    17641 aattcgaaag aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg
    17701 tggagatttt tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg
    17761 aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac
    17821 acgtagcaat cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg
    17881 tagcgaatat caaagcggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac
    17941 ttctggaagc tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa
    18001 tgggctacac gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg
    18061 aaccgcctta tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat
    18121 cgcacgtaac catgcctcaa atcagaggaa tgtataatgg tgaccgagcg agaaaacaga
    18181 tgctagtaga ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag
    18241 aagagtttga aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg
    18301 aacatgaaca aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc
    18361 cagtgatcga agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg
    18421 aacgagtcga aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag
    18481 acctcacaga ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca
    18541 aaacgctgga acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag
    18601 ttggaatcaa tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct
    18661 tggatgcgga taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac
    18721 gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta
    18781 tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat tcagcagaag tacaatgaag
    18841 aacatgggat cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga
    18901 aagaatcgga agatgataca aaagaagcgg tacaagtctc gtacgaagaa atgacgaagg
    18961 aagaaaaaga cacgttattg atgaaattag aaaaagaaat gaaagatgcg gcaaaagctt
    19021 tggattttga aacagccgcg aatgtgcgag atatgatttt agaattaaaa gcttcaaagt
    19081 aggaggacaa catggctaac gataaaatta cgattcatgg tgcacgtgcc cataacttga
    19141 aaaatataga tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt
    19201 ctggaaaaag ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag
    19261 agagcctttc tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata
    19321 gcatcgacgg attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc
    19381 ggtcgacagt aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg
    19441 taggacatcc gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa
    19501 tggcagatca agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg
    19561 tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg
    19621 tccgcgttcg agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa
    19681 acaaaaaaca tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt
    19741 ctcgcctatt tgattcttta gaggcggccc tgcgattagc agacggctat gctctggttg
    19801 acgtgatcgg tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat
    19861 ttactgttgg cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc
    19921 cagactgtga tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata
    19981 gaacgaagac acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt
    20041 atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg
    20101 aagaattacc ggaagagcat caggagatca tcttgaatgg gacagatgat cttttccatt
    20161 tccattatga aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat
    20221 tgaagaatat cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc
    20281 gtctttatat gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag
    20341 ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa
    20401 aagcagtaga tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac
    20461 cgattttaaa agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc
    20521 taactttgag tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg
    20581 ctacacaaat tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg
    20641 gattgcatca acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg
    20701 gcaacaccct cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatttga
    20761 ttgacgtcgg acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg
    20821 aagaagtagc taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa
    20881 tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt
    20941 cagaaaacaa tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca
    21001 caggtgtgtc tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag
    21061 cgcaaaaatt aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg
    21121 aaagtatcga aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta
    21181 atccagcgac ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg
    21241 aggcaaaaat gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt
    21301 gtgaagcttg tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct
    21361 atgttccttg tgaagtatgt catggtaaac gatataattc tgaaacatta gaagtgcatt
    21421 acaaaggaaa aagcattgct gatattttgg aaatgacagt agaggatgct gtagaattct
    21481 tcaagcacat tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt
    21541 atgtgacgat ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac
    21601 ttgccagtga attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa
    21661 cgacaggact tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag
    21721 atgctggtaa cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt
    21781 atatcattga tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa
    21841 caccagaaga aatcattaac gtaaaagaaa gttatacagg tcactatttg aaaaaaataa
    21901 tggtataaag aggatagata gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc
    21961 ttgttttatt aatatcagtg ttctagaatc attacatggt agttctttct acactgattg
    22021 gtatagataa aaacttccta taagttcaat ccgttcaaaa aagaattgag caatcggagg
    22081 actttccttt gattgctttt ttctttatgg tatgatattc cttgtgtagt tggaaaagaa
    22141 aaacaagaga ttatgagtaa gtgagagata atgatgaaca gtattgaaaa tgaagaattt
    22201 atccctatat tattaggaag cgacatgaac gtttatggta tggcacgttc ttttaatgaa
    22261 gcatacggca aaatctgcca agcatatgct tcggatcagt tagcccctac ccgctacagt
    22321 aaaattgtca atgtagaagt tattccagga tttgataaag accctgtatt tatcgagacg
    22381 atgcttcgtt tggcaaaaga aagatattct gataagagta aaaaatatct tttgattgct
    22441 tgtggtgatg gatatgcaga attgatctcc caacataaac aagagctgtc agaatatttt
    22501 atctgtccat atattgatta ttctttattt gaaaggctga tcaataaagt aagcttttat
    22561 gaagtttgtg aagaatatga tcttccgtat cctaaaacat tgatcgtcag agaagaaatg
    22621 ttagtaaatg gacatttgga acaggaattg ccttttgaat tcccagttgc tttgaaacct
    22681 gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta
    22741 gagactagag aagagttcga tttaatttta ggccggatat acgaagccgg ttataaaagt
    22801 gaaatgattg tacaagattt tatcccgggt gatgatagta atatgcgtgt gctaaatgcg
    22861 tatgtagatg aagatcatca agttcggatg atgtgccttg gtcatccact attagaagat
    22921 cctacaccag catcgattgg taattacgtt gtgatcatgc cagattataa cgaaaaaatc
    22981 tatcaaacca ttaaggcatt tttggaaaaa atcgaatata caggctttgc caatttcgat
    23041 atgaagtatg atcctagaga tggagaatat aaattatttg aaatcaattt gagacaaggt
    23101 cgtagcagtt tctttgtgac attgaacggc ttgaatctgg ctcgttttgt cacagaagat
    23161 cgagtgttca acaaaccctt tgttgaaacg acctatggaa cgaatcaatc agataaagca
    23221 agattatgga tgggtgtgcc caaaaaaatc tttttagaat atgctcggga aaatgaagac
    23281 aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg gtactactgt attttatgaa
    23341 aaagaccgat caatcaagcg ctggttgttg atgaaatata tgttccataa ctatattcca
    23401 agattcaaaa agtattttca tgtgaaagaa ggctagttca tggagaattt tttcagtatt
    23461 ttaggcggaa tgggcacgat ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg
    23521 aaagctacaa aagatcaaga atatttgaat tatgtcttat tcaatcatgc aacagttcct
    23581 gatcgtacag cttatatttt agatcgatca gaagaaaatc caatgccgtt tctgctggat
    23641 gatattgaga aacaaaattt attgcgacca aacttcattg tgttgacttg taacaccgca
    23701 cactactttt ttgaagagtt acaagcggca acggatattc ctatcttgca tatgccaaga
    23761 gaagcagcaa atgaacttgt ccgtcagcat acgacaggga gagtagcgat tctaggaaca
    23821 gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg tttcgaaaca
    23881 gtgattcctg atactgcgct tcaagaaaaa atcaattacc tgatctatca tgagatcaag
    23941 gaatcggatt atttgaatca agagctctat tacgaaatct tagaggaagc ggtagagcgc
    24001 ctaaactgtg aaaaagtcat cctaggttgc acagaattat ctttgatgca tgaatttgct
    24061 gaagataatc attacccagt gatcgatgct caatctattt tagcggatcg aacgatcgaa
    24121 cgtgcattag ctgaaagaag cgaggcattg gatacagcat cggaaaagta aaaaaattgg
    24181 atttggggca aatatcctaa aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa
    24241 actggtaatg atacgcccgg gatgatataa ttttattatg cacaatgtga gaggggttcg
    24301 taaatatgac agataatttg caattagtca tcataacagg tatgagtggt gcagggaaaa
    24361 cagtagctat ccaaagtttt gaagatatgg gctatttttg tatcgataat atgccgcctc
    24421 gattgattcc aaaattctgg gaattgatca aagaatcagg taaagtcaca aaaatggctt
    24481 taggagtgga tttgcgttct cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa
    24541 tcgaaaatac gaacttcatc gacacttcta tcttattttt agatgcttca gacgaagaac
    24601 tagtggctcg ctacaaagaa acaagacgag cacatccaat ggcgatggac ggtttgatca
    24661 ctgaagggat acgtaaagaa cgggcgatac tagacgactt gaaagcccaa gcttccgtga
    24721 tcattgatac gacaacattg acaccacgac aattacgtga aaaaatcaat gagtcattca
    24781 aatcaaaggg agaccaagga tttcggatcg aatttgtatc atttggtttt aaatacgggt
    24841 tgccgatcga ttcggatatt gtgatggatg tgcgtttttt gcccaaccct cattatatcc
    24901 cggaactaag accattgacc ggaaaagatc agccagtcta tgattatgtg atgtcctttg
    24961 atgaaacgca atcattctac cataaatttt tggatcttct ggaaactgta ttgcctggat
    25021 acattaaaga aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc
    25081 gttctgtggc attgactgaa cgtttggcaa atgctttagg gaaaaactat aaagtcaatg
    25141 tgacacatcg ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac
    25201 ctaccggata agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc tgccagttat
    25261 cctgaaaagt ttacgaaatc aaggagcaga tatcacagca gtagtgactg ttgcagatga
    25321 tggtggaagc agcggtgcaa tcagagaatc gatcgctatg gctcctcctg gtgacttgag
    25381 aaatgtctta gtggcgttat cggatatgcc acaattctat gaagatattt tccaataccg
    25441 tttcaaaaag gaagatcagt ttttagccaa tcatacgatc ggtaatctga tcattgccgc
    25501 agtttccgag atgcggggaa gcacttatga ggcgattcaa ttattagcaa aaatgatgca
    25561 tgtagatgga catgtttatc cttcttcaga aactcctttg accttgcatg ctgtttttaa
    25621 agacggaacg actgcagtag gagaatctaa aatcgcgatc aaccgtaaaa ccattgaccg
    25681 tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc
    25741 agcaattatg gacgccgaca tggtcgtttt gggacccgga agtttattca ccagtatcct
    25801 tccaaattta gtgataccag aaatcggcga agcaatgctc aagacaactg ctgaaaccgt
    25861 ctatatctgt aatatcatga cccaaaaagg ggaaacggaa catttcacag atgccgatca
    25921 tatacgtgtt ttacacaagc atttgaatca gccttttatc gatacagtat tggtcaatac
    25981 ggaaaaagtg cctgaagatt atatggaccc agagatctat gatgagtatc tagtacaagt
    26041 cgcacacgac ttcaacggat tgagaaatga aggatgtcga gtgatttcca ccgacttttt
    26101 agaattgaaa gatggtggtg tcttccacga tggagacaaa gtagtagaag aattgttccg
    26161 tttagttttc ggttcaaaat attaagcaat ttattgttgg agaaaggagg agaataggat
    26221 gtcatttgct gcagatgtga agaaagaact aactggtttg gaagttcatc gagaacatgc
    26281 gaaagcggaa ttagctgctt tgctgcgaat gaacggctct ttaggtattg tgaaccagca
    26341 gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct attctctttt
    26401 gaaagatcat tatcaggtac gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa
    26461 gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga tggatcttga
    26521 tatcatggat ggtatgatgt tcaatggaca cgtttcggac gagattatgg gaaattcgca
    26581 aaaaatgcgt tcttacttac gtggtgcttt tatggcctca ggttcagtca ataatccgga
    26641 aacgagtcgt tatcatttgg aaatattttc tatttatgaa cagcataacc aagacatttg
    26701 cgatatgtta aattattacg gtctacatgc tcgaacgctt gaacggcgca atggctatat
    26761 ttcgtattta aaaggagcag aaaaaattgc tgatttctta acattgattg gtgcaaccaa
    26821 ctcaatgctc cgatttgaag atgttcgaat cgtacgggat atgagaaatt cggtcaatcg
    26881 tctagtcaat tgtgagacag caaatctgaa caagacgatt gatgcggctt ctaaacaaat
    26941 cgaaaacatc gaattgatcg aagcaaaggt cggtctacat gcccttcctg aaaaacttca
    27001 ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat taggggaaat
    27061 gattccctca ggtgcaatat caaaatcggg aattaatcac cgaattcgca aaataaatga
    27121 atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg atacatgaag
    27181 caggagatgc gaatgtatca gtttttttta aaagctatac ttgcttttct tccgtcaaac
    27241 tataattcca actttactgg agtaacataa tgttgaaagg cttctattgt tttttaatac
    27301 aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttatac ataaactttt
    27361 gcttaatata gaagtcctga tatagatagg atcaagtatt tatctataac tggagattaa
    27421 ttaaaaactg gaaggaactg ctcgatcaac cttgagaact gtttttctta cttctggtca
    27481 agattctcgt ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa
    27541 acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga
    27601 taatcataag ttcataaata gtaattggga tgtatggttg gtatacgtta tacctgaact
    27661 tgaactatca agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa
    27721 atgtgctttt ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat
    27781 atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata
    27841 acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact
    27901 acagcaccac cattgccaga gattacatat ggagaatttc cgtttagtct tgaatatgaa
    27961 atagatgagg aaagaatttt gattgaagat acggtgattg cagaatttat aggaagtttt
    28021 agagggaatg tgacaactca acctagacga agttggagta taaaactttt aaataatagt
    28081 ggagagttga aagcatttca ttatgaattc ttaataatag aaagtgaaga tgtaacaatc
    28141 actttaagta gtggctctgc tggatatttt atgaatgatg gtggaagcaa gccacagctc
    28201 acagtccgtg atcgtattgc aggttttatg catttttctc actatgactt agaagaagct
    28261 cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt gggaatatgc gccaccgata
    28321 agaaattttt tcaatgatta ttattagtct tataaaaaac agaatgtaaa atttatgtgc
    28381 gcgagtattt agttttccat tttttgtttg atagatatat agcattgttg aagcatggga
    28441 tagtggtgcc aattttatca atgaggttgg tcaggcagtt aacataattg gtgatgctat
    28501 aaatccattt aattggaatt ggaggaatta ggtgtggaaa cagtgagatt atggatattc
    28561 atgaaaaatt ttcgttcggc tttgagatat gttattctgg aatcaaaatc aggttattat
    28621 ctgattgaca cagatcacta tactttttgg ggaggctacc tttttccgat ttttaattgg
    28681 ataatccccc ataaggttat gaaaattaac ttgcctgcag aagaaataaa tagattaata
    28741 ctcaacaaag atttacaaag gaaaagagag aaagaacatt ctaatgttgc ttggataggt
    28801 ccagcatttg cagtagtgac ttcttcaatt gtcggtccta tgctagagag tatttatagt
    28861 gatttttcta ttggaatacg ttatttattt gttactttaa gtatagctgt tcttttgtgc
    28921 ataaagggtt atgttagcaa gaagaataaa gatattttaa cactaatcgg tgtaaacaat
    28981 ttatctagta ctaaaattat actgtttcct gaatctataa aagaaggatc aaggttgttg
    29041 ataattttta taatgcttat actgttttct gttttatttg gggtgatgtt tataga
//
LOCUS       DAYYCQ010000030        28688 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000030 DAYYCQ010000000
VERSION     DAYYCQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.030"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..549)
                     /locus_tag="ACHAYF_001306"
     CDS             complement(<1..549)
                     /locus_tag="ACHAYF_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578755.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLN"
     gene            654..899
                     /locus_tag="ACHAYF_001307"
     CDS             654..899
                     /locus_tag="ACHAYF_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8578756.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1055..1906
                     /locus_tag="ACHAYF_001308"
     CDS             1055..1906
                     /locus_tag="ACHAYF_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8578757.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            1939..2616
                     /locus_tag="ACHAYF_001309"
     CDS             1939..2616
                     /locus_tag="ACHAYF_001309"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8578758.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            3104..4069
                     /locus_tag="ACHAYF_001310"
     CDS             3104..4069
                     /locus_tag="ACHAYF_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8578759.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            4091..4303
                     /locus_tag="ACHAYF_001311"
     CDS             4091..4303
                     /locus_tag="ACHAYF_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8578760.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            4378..4854
                     /locus_tag="ACHAYF_001312"
     CDS             4378..4854
                     /locus_tag="ACHAYF_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8578761.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4851..6659
                     /locus_tag="ACHAYF_001313"
     CDS             4851..6659
                     /locus_tag="ACHAYF_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8578762.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            6736..6951
                     /locus_tag="ACHAYF_001314"
     CDS             6736..6951
                     /locus_tag="ACHAYF_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8578763.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            7043..7906
                     /locus_tag="ACHAYF_001315"
     CDS             7043..7906
                     /locus_tag="ACHAYF_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8578764.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            7943..8479
                     /locus_tag="ACHAYF_001316"
     CDS             7943..8479
                     /locus_tag="ACHAYF_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8578765.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            8493..8915
                     /locus_tag="ACHAYF_001317"
     CDS             8493..8915
                     /locus_tag="ACHAYF_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8578766.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            8845..11244
                     /locus_tag="ACHAYF_001318"
     CDS             8845..11244
                     /locus_tag="ACHAYF_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8578767.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAVKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            11276..13267
                     /locus_tag="ACHAYF_001319"
     CDS             11276..13267
                     /locus_tag="ACHAYF_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8578768.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            13290..13541
                     /locus_tag="ACHAYF_001320"
     CDS             13290..13541
                     /locus_tag="ACHAYF_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8578769.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            13531..14760
                     /locus_tag="ACHAYF_001321"
     CDS             13531..14760
                     /locus_tag="ACHAYF_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8578770.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            14757..16838
                     /locus_tag="ACHAYF_001322"
     CDS             14757..16838
                     /locus_tag="ACHAYF_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8578771.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            16987..20907
                     /locus_tag="ACHAYF_001323"
     CDS             16987..20907
                     /locus_tag="ACHAYF_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8578772.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            20908..21852
                     /locus_tag="ACHAYF_001324"
     CDS             20908..21852
                     /locus_tag="ACHAYF_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578773.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            21856..22059
                     /locus_tag="ACHAYF_001325"
     CDS             21856..22059
                     /locus_tag="ACHAYF_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8578774.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(22359..22595)
                     /locus_tag="ACHAYF_001326"
     CDS             complement(22359..22595)
                     /locus_tag="ACHAYF_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578775.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(22778..23149)
                     /locus_tag="ACHAYF_001327"
     CDS             complement(22778..23149)
                     /locus_tag="ACHAYF_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8578776.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(23146..24474)
                     /locus_tag="ACHAYF_001328"
     CDS             complement(23146..24474)
                     /locus_tag="ACHAYF_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8578777.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(24435..24764)
                     /gene="mobC"
                     /locus_tag="ACHAYF_001329"
     CDS             complement(24435..24764)
                     /gene="mobC"
                     /locus_tag="ACHAYF_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8578778.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(25022..25392)
                     /locus_tag="ACHAYF_001330"
                     /pseudo
     CDS             complement(25022..25392)
                     /locus_tag="ACHAYF_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25537..25722
                     /locus_tag="ACHAYF_001331"
     CDS             25537..25722
                     /locus_tag="ACHAYF_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8578779.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            25880..26047
                     /locus_tag="ACHAYF_001332"
     CDS             25880..26047
                     /locus_tag="ACHAYF_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578780.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            26665..27324
                     /gene="vanR-B"
                     /locus_tag="ACHAYF_001333"
     CDS             26665..27324
                     /gene="vanR-B"
                     /locus_tag="ACHAYF_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8578781.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            27327..28670
                     /gene="vanS-B"
                     /locus_tag="ACHAYF_001334"
     CDS             27327..28670
                     /gene="vanS-B"
                     /locus_tag="ACHAYF_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8578782.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         7381 a         7309 c         7599 g         6399 t
ORIGIN      
        1 atttaatgtt agatttaatg gataatcata cgactttatt tttttcaact tcatattgta
       61 catatcttca gccataaaag ttgagttcgt gatttcatct tgggtctcat taaaaaagtt
      121 atccgaataa tcgcttattt ttgaaaagtc tttattggca ccagcctcaa aaaattgttc
      181 aactttttca gtataaagtt gtgctaacgc tcctttttca atattaactt taatattttc
      241 atgtctatca atttgtgtgc tgttcagtaa gggaagatca gcttttagtt ttatatcgtc
      301 ttgatttctt tcaatttcaa ttggcaaaat cttttcagta gattcagttt ctgataaagg
      361 aacatctagt tttttatcat gataataagc ttcaaatcca tctggaaccg gaaaaaaatg
      421 tattgggtaa ttcttttctg aggttgttgt actagtttcc gtacttgtag aacttgtgtc
      481 agaagtggta cacccgctta tagttccgat gagtaaagcg caagttagaa tgattgcaaa
      541 tttcttcaaa aaaattcctc cttaaaattg aatatggact aatactagtc ccattgtagc
      601 ataccgaatc tttaagatta ataagaaaaa ggactaattt tagtccaaaa ggtgtgctat
      661 tcttgaaaga ggaaaaaatt attgataaag aaaatgtaat cggtagcaat attagacgag
      721 tccgattaga aaaaggaatt ggtcaaaccg agctgattag agacctacag cttcgtaaaa
      781 ttgctataac tagggaaacg ttggttaaga ttgaaggtgg ccgccaacac attaagttgg
      841 atcaattgaa agccatcaaa gatattctac aggtgtctta tgaagatttg cttcaatgac
      901 tacttgtagt catttcgttt gaaattatta tgttacaccc ttatgtgtca cgaagaaata
      961 ctggtataat aatgaatggg atatgtaacg atagggtata ctgttatgtt gcctaataaa
     1021 ttgtggatca aaatacaaga atggagggat atccttgtat aaggcatatg aattggattt
     1081 tacaaatgct gacttaaatg cattctcctc aagctcgcaa tcatatacac atgtagtaaa
     1141 tcaaatagat cacaaccaaa aatctattaa caagagaata gaaaatctat ttgaaagtga
     1201 aaatgattta gttgattatt attctaagga cagcaaaatt ctagatgcag acgaaattat
     1261 taaagattgg tttccaacta taaaagcaga tatttttata tctcactctc atgctgatga
     1321 aaaattagct atacgttttg catcatggct atttgaaaat tttggtctta ctgcatttat
     1381 tgattcttcg gtttggggat acagttctga tttattaaaa aaaatagacc aaaaatattg
     1441 ctataaggaa caaacaaaaa catatgatta tgataaaaga aatgtaacaa ctagtcatgt
     1501 tcatatgatg ctttctacag ctttaaataa tatgattgat agtacagaat gtctgttttt
     1561 tcttaataca cctaattcaa ttagtttgag caatgaaatc actaatgaac agaaatttac
     1621 gtattcgcca tggctatata gcgaactgac gacagcttca atagtggaaa aaaagaatcc
     1681 acgattggaa agtaatcctc aaatgagcac cgaggatgtt agaagcatta taaagcacta
     1741 ttcttcgcaa gaaaatctgc aaataaaata ttctttaaat ttaagtgaat taacttattt
     1801 acgaaaagct gatttgaaaa aatgggcttt agatgctgtg gaatgcacta aaggaagtaa
     1861 tacattagat attttatata aaaacaccat tttcactaat ctctaatcaa tttactgaat
     1921 cgaaaggaac cgggtagaat ggataagaaa gttttaataa agaggttctc tgaagctatt
     1981 gaaaaaggaa atgcagcaat atttgcaggt gcaggattat cgatgtcaca aggatatgta
     2041 agttggccgg aactacttaa tgatcctgca acagaaatcg ggttggattc taaaaaagaa
     2101 acagacttag ttactctggc tcaatattat aaaaatgaaa atggtggatc aagaggaatt
     2161 ttgaatcaga tacttatgga taattttggt gaagaattag aaatatctga aaatcatcga
     2221 atactagcaa gtttacctat tgaaacttat tggacaacta attatgatca tctaatagaa
     2281 aaatcaatcc gagaagctta taaaaaccct caggttaaga aaaattatac tcagttagca
     2341 acaactaatc caaatgtgga tacaattgtt tataaaatgc atggtgatat agatgatgtc
     2401 agttcgacag taataacaag agatgattat gaaaaatatg atgattcgta cgcgttattt
     2461 aaagaaactt taaaaggaga tttgttaaca aaaacttttc tctttttagg atttagtttt
     2521 acagacccta atttagaaag aattttatca gatataagat gggttttgag agaaaaccaa
     2581 agacctcatt attgtataat gagaaaaata ttatagaaaa aattttaggt tatcgctgaa
     2641 agccccggaa atacgggcat ttcgcaggat atgagaactc aatttactac caatttacta
     2701 ctttcggatt gagccgcata tatgctgaac catactgcta atacccgcca ccccaaatac
     2761 ggcacatcat aaattgaata cgacaccgta aacgcggcgt tcctctttcc cgtcatggga
     2821 gaacaggacg ccgctttttt atgcccaaat agagaaagga ggcgacgcca tgctatttac
     2881 ttccggccct gaccgggcgt ttgaacggct gatgcaggaa aagcccggtt tcgatcacta
     2941 cgacggcggc ggggcggacg cgccggagga ttgcggcacc tgccgatttt accgcccgga
     3001 ttggaaatat caattctgcc agtttgcaga gtgtccctat cagccgggca agctgaccga
     3061 ctatgcagcg gtcaaattca cagtgaaagg agcgaacaac gctatggcag ttttccgtgt
     3121 ggaaaagaac aaaggctata cggtgatgtc aaatcaccac ctgcggaata agaacctgac
     3181 cttaaaggcc aagggcctgc tgtcgcagat gttgtccttg ccggaggatt gggactatac
     3241 cctaaaagga ctgtcctata tcaaccggga aagcatcgac gcgatcagga cagcggtatg
     3301 ggagcttgaa aaagctggat atatccggcg tgagcagggc cgtgacgcaa aaggcaaaat
     3361 ggccgatatg gtctatacca tctacgaaca gccggtattg gaaaacccgg tattggaaaa
     3421 tccaacatcg gataacccgg tgttggaaaa tccgacatcg gataatccgg cgtcggaaaa
     3481 tccaacgcaa ttaaataaag aagtacaaag aactgactta tcaaaaaaag aaaaaataat
     3541 tacagatgga caaagtaccc attccattcc tatcccatcc ccgacccctg cccctatgga
     3601 gggcgaggca gcggctacgc cgccggaacg gaaaaggaca ggaaaggatg aggctgtacg
     3661 gatttatcgg gaaatcattt tggagaacat cgagtatgcc tatctcattc aggacaggag
     3721 cattgaccgt gaacagcttg acgaaatcgt agacctgatg ctggaaaccg tctgcaccgc
     3781 ccgaaagaca atccgtgtcg ccggggacga ctaccctgcc gagctggtga aatccaagta
     3841 catgaagctg accggcgaac atatccgctt tgtccttgac tgtctgcggg agaacacaac
     3901 gaaagtccgc aatatcaaac aatacctgcg ggctatgctg ttcaacgccc ccagcaccat
     3961 ttccaactac tacacggccc ttgtcgcgca cgacatggca cagcccgatt ggggaaagcc
     4021 taaatccggc ctgccggact attcctgcgg cgagggcgaa agcctgtaac gagaaaagga
     4081 ggacgaccct atgaagcagg gcgcattgat ttttgacgag cgaacagacc gttacgacat
     4141 ccgctttgat ctggccgact attacggcgg cctgcattgc ggggagtgtt tcgacgtgat
     4201 gatcggcggc aaatggaggc ccacccgcat tgaaatggcc gaaaactggt atcttgtcgg
     4261 catccgggcc gacgaccttt ccggcctgcg ggtgcggatt tgacctatat ctacctgcgg
     4321 cgtctggtgg ttccgcctta gacgccgctt ttgactttct gacaaggagg gatgcgattg
     4381 caggaagaag ttactcaaaa gaccattgcc ctgtccatga aaacgggcaa gctcacggct
     4441 caagtgctgc aagcggcgct gaaaaagtac ctgcaacacc gggccaaggg caaaaacacg
     4501 ctgcaccacg gccagcagag cctaaagcag cttaaaaagc atggggctga tctctccaac
     4561 attgagatca ccgaggccaa cattggagcg ttcaagccat gcgccaagaa atacggcgtg
     4621 gattttacct tacgaaaaga taagaccacc cagccgcccc actacgttgt gattttcaag
     4681 gccaaggatg cagataacct tgatctggcg tttcgggagt ttaccgccaa gacgctttcc
     4741 aaggagcagc ggccctctat ccgcaaggtt ttgtccgctg tgaagcaaaa ggcggcggcc
     4801 cagaccaagc agcgggccaa ggagaaaatc aaggaaaggg ggctggaact gtgaagcctg
     4861 acgtaaagaa attgctgatc ctgaacctcc cgtatctgat ttttgtgtac ctgtttgcga
     4921 aatgcgggga ggcgtaccgg ctggccgctg gtgctgacct gtcggggaag ctcctgcatt
     4981 ttgcggatgg ttttgcggcg gcttttgcca acccgctgcc aagcctgcac ctgtttgacc
     5041 tgtgtatcgg tatcgccggt gccgtgatcg tgcggctggt ggtgtactgc aagagcaaga
     5101 acgtgaagaa ataccgcaaa ggcatggagt atggcagcgc ccgctggggg acggccaaag
     5161 acatagcgcc ttacatcgac cccaagtttg aaaataatat cctgctcacc caaacggaac
     5221 ggcttaccat gaccggcagg cccaaagacc ccaagacagc gcggaataaa aacgtgctgg
     5281 tgatcggtgg cagcggttca gggaaaacga gatttttcgt gaaacccaac ctgatgcaat
     5341 gctttccgac cacggactat ccgacttcgt ttgtggtgac agatccgaaa ggcactttgg
     5401 ttctggaaac cggcaggatg ttccagcagg cgggctaccg cattaaaatc ctgaatacca
     5461 ttaacttttc caagtccatg aagtacaacc cctttgtcta catccattcg gaaaaggacg
     5521 tgctaaagct ggtaaatacc ctgatttcca acaccaaggg cgagggggaa aagtcagcgg
     5581 aggatttttg ggtgaagtcg gaacggttgt tttattcggc cctgatcggc tatatctggt
     5641 acgaggcccc agcggaggaa atgaacttct caacgctgct ggaaatgata aacgccagtg
     5701 aagcccgcga ggacgacccg gagtttcaaa gccccgtcga tctcatgttt gagcgtttgg
     5761 agcagaaaga ccccgaccat tttgcggtgc gccagtacaa gaaattcctg ctgtccgcag
     5821 gcaagacccg ttcttccatc ctgatttcat gcggtgccag acttgcgccc tttgacatcc
     5881 gggaggtacg cgagctgatg gaggacgacg aaatggagct ggacaccatc ggggacgaaa
     5941 agacggtgct gtatctcatt atgagcgata cggacacgac ctttaatttc attctggcta
     6001 tgctccaaag ccagctaatc aacctgcttt gtgaccgggc tgatgatgtg tacggcgggc
     6061 ggctgcctgt ccatgtacgc ctgattttgg atgaatttgc caacattggc caaataccca
     6121 atttcgataa actcattgcc accattcgaa gccgtgaaat atcggcctct attattttgc
     6181 agagccagtc gcagctaaaa gcgatttaca aggacgccgc cgagatcatt tcggataact
     6241 gcgatagtgt gcttttcctt agtgggcgcg gcaaaaatgc caaggagatt tccgatgcgc
     6301 tggggaaaga aacgattgac agtttcaaca ccagcgagaa ccggggaagc caaacgtccc
     6361 acggattgaa ctatcaaaaa ttaggaaagg agttgatgtc ccaagacgaa attgccgtga
     6421 tggacggcgg caagtgtatc ttgcagctac ggggtgtgcg cccgttttta tcggagaaat
     6481 tcgatattac caagcatccc cggtacaaat accttgccga cgccgacaag aagaatacct
     6541 ttgacgtgga cagatttttg actatcacgc gccggaaacg gcagcaagtg gtaacgcagg
     6601 atgaagtatt cgacctgtat gaaatcgacc tgtcggacga ggacgccgcc gctgaataaa
     6661 cagcggcttt tttcattcca acaacaaaat caaaaaatca ttatgagccg gaaaccggca
     6721 gaaagaggta aatttatggc attttttaat tccgcagtta ctactttgca gactatcgtg
     6781 atcggactgg gcggcgctct gtgcgtatgg ggcggcatca atctgttgga gggatacggg
     6841 caggacaatc cgggggcaaa atcgcagggt gtcaagcagc ttgtcgctgg cggcggcgtg
     6901 gccctgatcg gtgtcactct ggttcccctg ctgtccgggc tgctgggctg atcctggcct
     6961 ttgtccctgt ccaaaccctt aactaatcgc ctccgcgtcc tcccggccag accgggaggg
     7021 cgcgggaaag gaggtgctac ccgtgattag tgaaatcatt gaagaatgga taaaaggcat
     7081 cctcatggac ggtatcacgg gcaacctgtc cgggctgttc gatacggtaa atgccaaagt
     7141 cggagaaatc gccgccgacg tgggacgaac cccgcaggca tggaacagcg gtattttcaa
     7201 tatgctgcac agcctgtcgg aaaccgtgat cgtccccata gccggggcca tactcgccct
     7261 tgtcatgtgc tatgagctta tccagatgat aacggaaaag aacaatatgc acgactttga
     7321 cagctccatg ttcttccgct ggatattcaa atccgcgttt gcaatcctga tcgtaacgaa
     7381 cacatggaac atcgtcatgg gcgtattcga cgccacccaa agcgtcgtca atcaaagcgc
     7441 cggtgtgatt atcgggcaga catccattga ttttaacacc ctgataccag accttgaaag
     7501 ccaattggag gctatgagcg tgggcgctct tttggggctg tggttccaaa cccttgttgt
     7561 gggcctcacc atgaatatcc tttccatctg cattttcctt gtgacctatg gccgcatggt
     7621 ggaaatctat gttgtcacgt cgctggggcc tatccccctt gccacaatgg gcaacgcgga
     7681 atggcgcggc atgggccaag ggtatctgaa atccctgctg gcgctgggct tccaagcctt
     7741 tttaatcatg gttgtggtgg gtatctacgc ggtgctgata cagaatatcg gttccgcttc
     7801 cgacatatcc ggcgcaatct ggggctgtat gggctatacg gtgctgctgt gcttctgcct
     7861 gttcaagacc ggcagtatat caaaggccgt attcggggcg cattgatccc aaaatccaag
     7921 catacacagg aggaacttgt ttatttcaac gtacaacatc atttacgctg acccgccgtg
     7981 gcggtatgac tgcaaaaacg tacagggcgc agcggaaaat cactattcca ccatgagcat
     8041 tgacgagctg tgcgccctgc cggtggaacg gctggcgtcc aaagactgcc tgctgttttt
     8101 gtgggccaca ttccccatgc tgccggaagc tctgcggtta atcaaagcgt ggggcttttc
     8161 attcaagacc gtggcttttg tatggctgaa acagaaccgt aaaagcctta catggtttta
     8221 cgggctgggc cgctggacaa ggggaaacgc ggaaatctgc ctgctggcaa cacacggcaa
     8281 gccgaagcgt cgttctgcct ctgtccatca gtttattatc agcccgattg aacaacacag
     8341 caaaaagccg gatgtgaccc gtgaaaaaat cgtagagctg gcgggcgatt tgccccgcgt
     8401 ggagctgttt gccagacaga aaacccccgg ctgggacgtg tgggggaacg aggtggaatg
     8461 tgaccttatc ctgacgtgaa aggaggtgcc aaatggctta tgtaaccgtc cccaaggact
     8521 taacccatgt gaaaagcaag gttgtgtttg ggcttaccaa acggcaactg atctgttttt
     8581 ccggggcgct ggtgatcggc gtccccttat attttctcac ccgtgactat cttaccaaca
     8641 gcgcggcggc cctgctcatg gtatttgcca tgctgccggg ctttttgctt gccatgtacg
     8701 agcgtcacgg ccagcccctt gaagtggtga ttatgcagat catccagtgc tgcttcatcc
     8761 agccaaagga gcgtccctat cagaccgaca acacctacac cgcccttgtg cggcaatacc
     8821 agatggaaca ggaggtaaag gccattgtcc aaaaaggaaa tcccaagaga aaccgaaaaa
     8881 acaaagctga cccgcgccca gcgaaaagaa attgacgcgg taatccgtaa atataagggc
     8941 gacggcaggc cccacacggc gcagcagagc atcccctacg aggtcatgta cccggacggc
     9001 gtgtgccggg tatcgcccgg cgtcttttcc aagtgcattg agtttgcgga catcagctat
     9061 cagcttgccc agccggacac gcagacggct atctttgaaa aactgtgcga cctgtataac
     9121 tatgtggacg cctccattca tatccagttt tctttcctca atcggaaagt tgacccggtg
     9181 cagtacgcca agagctttga gatcgcgccg cagggggacg acttcgacga catccgggcc
     9241 gagtacaccg gcattctgca aaagcagctt gcaaacggca acaacggcat ggtaaagacc
     9301 aaatacctga cattcaccat cgaggccgaa agcgtgaaag cggcgcgagc cagattgaag
     9361 cgcatcggct ttgacctgtt aggctatttc aagagcatgg gggccgtagc ccatgtgatg
     9421 gacggctggg aacggctgaa cctgctgcat ggcgtgtacc acccggacgg cgagatattc
     9481 aattttgact ggaagtggct ggccccgtcc ggcctctcca ccaaggattt tattgccccg
     9541 tcctccctgt gcttcggcaa tgcaaagaca ttcggcatgg gcggcaaata cggcgcggta
     9601 agtttcctgc aaatcctgtc cccggagctg tcggacgata tgctggccga cttcctcaat
     9661 acggaaagcg gtgttttagt caatctccat gtgcaggcca ttgaacagac caaggcggtc
     9721 aagaccatca agcgcaaaat taccgacctt gacgctatga agattgcgga gcagaaaaag
     9781 gccgtccgca gcggttacga tatggacatt cttcccagcg accttgccac ctacggcgag
     9841 gacgccaaga agctgctgac aaagttgcag acccgcaatg aacggctgtt ccagttgacg
     9901 ttccttgtcc tcaacgtggc cgacaccaag caaaagctga ataacgacgt gttccaagcc
     9961 gcaggcgtgg cccaaaagca taactgtccc cttgtccggc tggactacca gcaggaacag
    10021 ggccttgcaa gcagcctgcc gctgggcgtc aatcagatca agatacagcg cagccttacg
    10081 acttccagcg tggccgtgtt cgtccccttt gtgacgcagg agctttttca gggcggcgcg
    10141 gccatgtatt acggcatcaa cgccaagtcc cgcaacatga ttatgctgga caggaagcag
    10201 gcccgttgcc ccaatgcctt gaagctcggc acccccggca gcggcaaatc catgagctgt
    10261 aaatccgaga tcgtcagtgt gttcttaacg acgcctgacg atatttttat ttcagacccg
    10321 gaggccgaat attaccccct tgtcaaacgg ctccacggac aggtaatccg gctgtcgcct
    10381 accagcaagg actttgtgaa cccgctggac atcaacctga attattcgga ggacgataac
    10441 ccgctggccc tgaaatccga ctttgtgctg tcgttctgtg agcttgtcat gggcggcaag
    10501 aacgggctgg aagccattga aaagaccgtg atagaccggg ctgtgcgcgt gatctaccgg
    10561 ccctatctgg cagacccccg cccggagaat atgccgatcc tgtccgacct ccacaaagcg
    10621 ttgttagatc agcatgtccc ggaggctgac cgggtggcgc aggcccttga cctgtatgtg
    10681 tccggctccc tgaacgtatt taaccaccgt accaacgtgg acatcggcaa ccggcttgtg
    10741 tcctttgaca ttaaggagct gggcaagcag ctcaaaaagc tggggatgct cattgtccaa
    10801 gaccagatat gggggcgcgt cacggccaac cgaagccaag gaaaggccac atggtatttt
    10861 gccgacgagt tccatttgct tttgaaagag gaacagacgg cggcctattc tgcggagatt
    10921 tggaagcggt ttaggaaatg gggcggcatt ccaacaggtg ccacccagaa cgtcaaagac
    10981 ctgctttcct ccccggaaat tgaaaatatc cttgaaaaca gcgacttcat tacgctgctc
    11041 aatcaggcgt ccggggatag aaaaatcctt gcggaacgct tgaatttgtc caccgaacag
    11101 cagaagtaca tcgacaattc cgaacccggc gagggccttt tgatctttga aaacgtggtg
    11161 ctgcccttta ccaaccccat cccgcacaac acccagcttt acaagattat gacgacccgc
    11221 ttaaacgagg tggcgggagt atgacccggc gacgccacaa aggaggtgaa ccaccatgaa
    11281 agagctgaaa gccaaggcga aagtgacgca ggccatgacc cgcgacgggc tgacgctgga
    11341 aaatcaggcc accggcgagg tggagaacat ttccagccgg gaggccgaac aggatttgtc
    11401 cgcgccgggg ccgggcggca ccgccgataa gctgctggaa cgggccgacg ccatccatga
    11461 cggccataag gccaagaaag ccgcgaagca ggccggggct gtggccgccg agggggacgc
    11521 tgccctgcac cggccatccg cccggctcca actgtcggag gaagaacggg ccgacccgga
    11581 tttacagccc tatatccgaa aatcggacaa acgggccgat aagctggata aggcccgcgc
    11641 cgccatcccc aagaagcgcg tcccggtcaa ggaaaccgtc tttgacgagg caagcggcaa
    11701 agcaaagacc gtcctgcact ttgacaaaca ggacaagggg cctccctccc tgaaacccaa
    11761 tccggcaggc cgtcctgttt ccgaggtttt tctattcggc cacgggaaaa tccatgaagt
    11821 cgagcatgaa aacgtgggtg tggagggcgg ccataagggg gaggaacttg ccgagcggca
    11881 ggccggaaaa gccatccgca gcaccatccg gcaccacagg ctgaaaccct actgggacgc
    11941 ggaaaaggcc gagcgcaggc tattgtctgc caacgcggag tatttctacc agaaatcttt
    12001 gcgggataac ccggagatcg cacaggcggc cagcaacccc atttcccggt tctggcagaa
    12061 acagcagatc aagcgccagt acgccaaggc cgcccggaca gcgggccaga ccgcaggcgc
    12121 ggcggcacag ggcgcggcca agacagccgg ggcctccacg aaagcggcca aggccgcagc
    12181 caaagaaagt gaacgggcgg cctcctttgt ggcccgccac tggaaagggg cgctggccgt
    12241 ccttgtgttg gggcttttga tgctgcttat catcggcggc ttacagtcct gcaccgccat
    12301 gttcggcagc gccggaagcg gcatcacggc gtcctcttac ttttccgagg atagcgatat
    12361 gttggccgcc gaggacgcct acgcggagct ggaagccgag ctgcaagact atctggacaa
    12421 ctacgaggcc acccatgact atgacgaata ccattttgac cttgacgaga tcagccatga
    12481 cccctatgtg ctgatttcca ttctgtcggc gctccatgac gggatattta ccatcgggga
    12541 ggtacagggt gatttgtcca tgctctttga caagcaatat atcctgacag aaaacgtcgt
    12601 ggtagaaacc cgttaccgca ccgaaaccga tacatggacg gacgcggagg gcaacaccca
    12661 tacagacacc taccaagtgc cgtatgacta ttacatctgc tacgtcacgc tggaaaactt
    12721 cgacctgtcc catgtgccag tctatatcat ggacgaggaa acgctaagtt tctattccct
    12781 gtatatgtcc acgctaggaa accggcctga tctgttttcc ggcagccagt accccaacgc
    12841 ctccaccctg acgccgccga cctactacga cgtacccccg gaggctttgg aggatgccac
    12901 ctttgccgcc atgctcaaag aggcggaaaa atatatcggt tatccctatg tgtggggcgg
    12961 cagctctccc agcaccagct ttgattgcag cggcttcatt tcgtgggtaa tcaatcatag
    13021 tggatggaac gtgggacggc tgggggcgca gggcctatat aacatctgca cccctgtttc
    13081 cagttcgcag gccaagcccg gcgaccttgt atttttcaag ggaacctacg acacccccgg
    13141 cgtgtcccac gtcggcctgt atgtgggcaa caatgtgatg ctgcattgcg ggaacccgat
    13201 ttcctacacg aaccttactt caaactactg gcaatcccat ttttacagtt acgggcgttt
    13261 accttaacgc cagaaaggag ctttcaatta tggcaaccaa acttgaccgc atcgaaaagg
    13321 acatccagaa aaccaagtct aagatcgcgg agtttcagaa acagcttcgg gagctggaaa
    13381 cgcagaaaac cgaacaggag aatttgcaga tcatccagct tgtgcgcggc atgaacatga
    13441 agccggagga atttgccgcg ttcttacgca gcggcgcaat gcaggccgcc cctgccgcaa
    13501 ccccttacca tgaacaggag gacaatgccc atgaagaata agaccagacg gatttcccgt
    13561 attctccccg cacttctggc cgtgatgctc tgcgtcacgg cctttcccgt taccgccctt
    13621 gcaggcggca atgacccggc accggccccg ctgccggagg ctacaaccga acccgctacg
    13681 ggcggtttgg agcctgaaac agacgaaacc ggcaccgcca cggacgggac agaaaccgag
    13741 gccgaaaaag ataccggccc tctgaccggc aaggacatca ccgacctgtt ttccgcgctg
    13801 ttcggttcca aggtgagcat tgccgccacg gacgacggca tccagatcac caccggcacc
    13861 gacaaaaaag aacctgaaca gaccggcacc gtcaccacca acggcggcag gctgaatgtc
    13921 cgcaccggcg cggggcttga caagaccgcg ttcacccagc ttcccaatgg gacgacggtt
    13981 gaggtagtcg gcacagacgg cgactggatt aaaattctct tgccggagcg catcggctat
    14041 gtccattccg actatatgac cgtcagcgaa aaggaagtag cggctaccgg cgagggcggt
    14101 ttttccctct ccattgaccc ggaggaaatt gcttctctgc tggaactgtt caatggcggc
    14161 ggggccagcg ccgccctgtc cccggacggg aacctgtcac tgattgacga catcggcagc
    14221 gccgcgaaaa atggcaagca gtttatcacc gtggaaacca aaaacggcaa tgtgttttat
    14281 ctgattattg accgggacga cgagggaaaa gaaactgtcc atttcttaaa tcaggtggat
    14341 gaagccgatc tgctgacgct gatggaggac ggggaaaccg tcacccccgc tgctgtctgc
    14401 tcctgtacca cgaaatgcaa ggccggggct gtgaacacga attgcccggt atgtaaaacc
    14461 aacctgaccg agtgcagcgg cccggagccg caggaacccc agccggagga acccgaagcc
    14521 ccggaggaag aacccaaggg cggcgcgggc ggcctgatcg tgttcctgct ggtggcgctg
    14581 gcgggcggcg gtgcggccct ttactatttc aagttcaaaa agcccaaggc tgaaaccacc
    14641 ggcagcgacg atttgggcga gtatgatttt ggggaggacg aggatttgga ggacgaggaa
    14701 cccgaaaccg agtttgaccc ggaagctgat tttgtgagtg aacaggagga cgaggaatga
    14761 catataaact tgtaatcgcg gaaaaaccca gcgtggcccg cagcattgcg ggcgtgatcg
    14821 gggccacgga gaaacacgac ggctatttgg aggggaacgg ctatctggtg agctggtgca
    14881 tcggacatct ggtgtccttt gccgacgccg gacgctatga cgagcgtttc aaaaaatggc
    14941 gctatgagga tttgcccatt ctcccggaaa catggcagta catcgtcccc gacgacaaga
    15001 aacagcagtt tgacgttctc tgttcgctga tgcagcgccc cgacgtgacc ggccttgtgt
    15061 gcgccaccga cgccgggcgc gagggggaat tgattttccg tttcgtctac cagatggcgg
    15121 gatgccaaaa gccctttgag cgcctttgga tttcctctat ggaggacagt gctattaagg
    15181 acggctttgc ccacctgaaa ccgggcggcg actatgatcc gctgtatcaa tcggccctgt
    15241 gccgggccaa ggccgactgg ctgatcggca ttaacgccac ccggcttttt tccgtcctgt
    15301 accacaagaa cctgactgtg ggccgcgtcc agacgccgac cttaaaaatg ctggtggaca
    15361 gggacgccaa gattaccgat ttcaagaaag agaaatatca catcgtccat atcacggcgg
    15421 gcggcgcgga cgcggtaagc agccgctttt ccgacgctgc cgaggcaaac accgtcaagg
    15481 cggcttgtgc aaaagcgcag gccgtttgcg tttccgtgac gcgggagaaa aagacggaac
    15541 agccgcccaa gctctatgac cttaccacct tgcagcggga ggctaaccgg cttttcggct
    15601 ataccgccaa gcagaccctt gactatgccc aggcactgta tctaaagaag ctgctgacct
    15661 atccgagaac ggatagtcaa tatcttaccg acgatatgct gcccgccgtg gaaagccttg
    15721 tgtccggcct gtgggagctg gtgccgtttg ccaagggcct gaacctttcc ccgcagtttg
    15781 accgcatcct gaacagcaaa aaggtgtccg accaccacgc cattatcccc accgctgaat
    15841 ttgtgaagca gggctttggc actctggccg agagtgaaaa aaagctgctg tccctgatct
    15901 gctgcaagct cttatgtgcc gtggccgggc cgcatatcta tgaggccgtc accgccacat
    15961 ttacctgtgc cgggcaggag tttaccgcca agggcaagac cgtcctttcc cccggctgga
    16021 aagagattga acgccggttc cgttcttcct taaagaccga cgccgacgag gacgccgagg
    16081 cggtgcggga gctgccggag ctttgcgagg gccagacctt tgcagacgtg gccgcctctg
    16141 tcaccgagca tttcaccacg ccgcccaagc cctacaccga agatacgctt ttgtccgcta
    16201 tggaacgggc cggggccgag gatatgccgg aggatgccga gcgcaaggga ttaggcacac
    16261 cggccacccg cgccgccatt ctggaaaagc tggtgcagat gggctttgtc cagagaaaag
    16321 gcaagcagct cattcccacc aaggacggca tcaatctggc tgtggtgctg ccggaggcgc
    16381 ttacttctcc ccagcttact gccgaatggg aaaaccggct gaccgagatc gccaaggggc
    16441 aggccgatcc tgacgaattt atggacggca tcgagacgca ggcgcgggag ctggtgcaga
    16501 cctattcctg tatcagtgag gaaaagcaga aattgtttca ggccgagcgt gttcctatcg
    16561 ggacgtgtcc ccgctgtggt gaggctgtct atgagggcaa gaaaaactac tactgcggaa
    16621 accgggcctg ccagttcgtc atgtggaaga atgaccgatt ttttgaggaa cgcaagaaag
    16681 cgttcacccc gaagatcgcc gctgcgctgc tcaaaaccgg caaagtcaag gtaaagggcc
    16741 tctattccgt aaagaccggc aaaacctacg acgggacggt gcttttggct gataccgggg
    16801 gcaagtacgt caactaccgc attgaacaca ggagctaaag gacagtaaca cagcaagcat
    16861 agggagtgtg gctacactcc cggtaaaatc ccccggccgc gtccctgccg ggccggggat
    16921 ttttgcttgt tggggaagtc cccaaccccc tattttttga aacagaacag agaaaggagt
    16981 gaccgcgtga acgaagtatt ttctatccaa gtccataacc ggcagttttt ggaacagggc
    17041 aaacaaggcg tctggctgga tttgcccacc acaaccgaaa cgctgcaagc cgccctgcgg
    17101 gatgttggca ttactgccga caatccgcag gattttttta tcaacgggta ttcctgcccg
    17161 gaggacagac accttgccat tccctatgac atggtgctgg ccgccgacgt ggacgagctg
    17221 aatttccttg ccgcccggct gggccagctt gacgccgccg agatcgcaga gctgaacgcc
    17281 gccctgcaaa atccccgtgg cggctttgag aatatcggcc agatcatcga ctacaccgaa
    17341 aatgtggatt actatgtgca tcttcccgac gttagggggg atggacagtt gggcgactac
    17401 tacctgaacc gttccggcat ggtggatatg ccggaggaat ggaaagccgg gattgatacg
    17461 gcgcagtttg gacgccacat tgcccagcag gaacaaggcg tttttaccca gtacggctat
    17521 ctggtgagaa gcggcgacga atggcagcgc gtccatgagg gccagccggt gccggaggaa
    17581 taccgggtga tgcactaccc ccagccggaa gctgaccggg aggaaccgca ggcccagcag
    17641 ccggaggcat ccgaaagccc cgacaaagcg ccgcccccgg ttatcccgat catgctggac
    17701 ggcatgaaca gcaaagaacg catgaaagtg attactgacc gtctggaaac cggcgtacag
    17761 gagctttttg aaagtagccg ctatatggct tacttatccg ctatgtccaa gatgcacgat
    17821 tacagtctga acaatacgct gctgatcgcc atgcagggcg gttcgattgt ggcgggctat
    17881 aacaggtggc aggatgaatt tgaccgccat gtgaagcggg gcgagaaagg cattaagata
    17941 ctggccccgg ccccgttcaa ggttaagcag cagatggaga aaaccgaccc ggccacccag
    18001 cagcccgtga tcggcgcgga cggcaagccc gtgaccgaag aacaggaagt gacggttccg
    18061 gcctttaagg tggtgacagt ctttgacatc agccagaccg agggcaagga ttttcccatg
    18121 ccctatatca gtgaacttac cggcgacgtg cagcagtatc aggatttctt tgccgcgctg
    18181 gaacggacat caccctatcc cataagcatt gagccgattt cagacggggc caagggccgc
    18241 tgcttctacg caaaacagcg cattgccatc aacgagggca tgagcgaatt gcagacccta
    18301 aagaccgcca tccatgagat tgcccatgcc aagctccacg ccattgaccg ggaggccccc
    18361 gccgaggaac aggcggcccg gccagaccgc agcacccgcg aggtacaggc cgaaagcgtg
    18421 gcctataccg tctgccagta ttacgggctg gacacgtcgg actattcttt tggctatgtg
    18481 gccgattgga gcagcggcag ggagttatcc gagctgaaag cctcccttga aaccatccgg
    18541 gccacggcca gcgaattgat aaccgagatc gacggccatt ttgcccagct tcagcaggag
    18601 cgcgaggccc ggcaggaaca ggaggccgtc acggttgacg ttaccccgga ggcagcggcc
    18661 ccggcgcagg ccgaggaaga acaggccccg gcccttgatc cggcagccga gccgcttgtg
    18721 actgtgctat ggagcgaaag cccccagcta aaggacggcc agcaaatgcc gttgcatgag
    18781 gccgacgcgc tgtttgcccg tctggatgca gcatatccgg cagaggcagg ctatgacaaa
    18841 accgcgttcc gcattgactg tatctttcag ggcgagccgg acagctacga ggggcggcag
    18901 gattttggag atagggacgg ttccctgatc gaacatatcc agagctacca ccaattttac
    18961 gcgcaggacg aaaactggaa aaagcatgta atcgctacaa ccggcataga gggctgggag
    19021 caggacaagg cccatcggga tatgctgtta ggcgagtttg tcccctatat gaagctgcat
    19081 tgcagccttg cccaaatgga acagacggcg cagggccgct tacagtccgg ggaaaccctg
    19141 acgccggagg aaacggccta tttgggggcg cttctctctt atgtgcagga gtgccgcccc
    19201 atgctcaatc agggccagta tcagttgccg gagccgccga aactggctga cttcgaccag
    19261 agcttgcagg actacaaagc acaggtacag gccgagatcg cgcaggaggc cgccgcagcc
    19321 ggtatgaccg tggaggaata cgcggcggct ggctatgaag caccccaaca ggacaccttt
    19381 tccatctacc agcttcgagg cggggaggaa acccgcgact atcggtttga gccatacgag
    19441 cgtttacagg ccgcagggct ggccgtggac agtgccaact atgaccttgt ttatacggca
    19501 ccgctggata aaagcacgtc tttggaggat atttaccgca cattcaacat cgaccacccg
    19561 gcagatttta ggggacacag cctttccgtt tccgacgtgg tggtgctgca ccagaatgga
    19621 caggacaccg cccactatgt agacagcttc ggctatatgg aagtgccgga gtttttgcgg
    19681 gaaaatcccc tgcgtaccgc cgaactgtct acggagcaga acgaaaacat gattgacggc
    19741 attatcaaca atacgccgcc cgaaccgcag ccggaggaac ccgaacagct atcttttgag
    19801 gatgtggcag ttgcttccgg ccagacacag gaggaacccc cggaggctgc gcccccgcaa
    19861 acggctgtcc gctattatcc catcaatgag ggagccgccc gccgcgccaa ggaagccatg
    19921 agcttttctg actaccggcc cggcagcgcc acggcggaat accggcacta tgtcgatcag
    19981 gccgtggaaa ttgcggagcg ccagaaaaag cgtgtggagc cagagtatca cgaaaagata
    20041 gacagcctcc ttgacaccta cgcccgcaag ctggccgaga acatgaataa gggctatgag
    20101 attaccgccc gcgtcccctc tgtcctgatt gccgggccgt ccaatttccc ggtacgggcg
    20161 aaagaaaagc agaacgccgc cagtgaccgc aacatggagg aatttcagta cattcagggc
    20221 ttgcttgaca aaatccgcag caccggcatg ggtggtatca gcgccgacga tccgcaggcc
    20281 gtttccaagc tggaaaagaa actggaaaag ctggaagcgt cgcaggagtt gatgaaggcc
    20341 gtcaatgcct actaccgcaa gcatgggacg ctggacggat gcccccatct gatcgaaaga
    20401 ggcattgaaa acctgaaagc ggatatggcg tcgggctggc actatgagaa aaaaccgttc
    20461 cagtcctggc agctctccaa caataacgcc gagatacgcc ggttaaaggg ccgcattgag
    20521 gaattgaccc gccaaaagga ggcggcctat gtgggatggg agtttgacgg cggcactgtg
    20581 gagatcaacc gggtggccaa ccgcctgcaa atcttctttg aggggaagcc ggatgctgct
    20641 gtacgcgacg agctgaaaag caacggtttc cgctggtcgc ccaaggccgg ggcatggcag
    20701 cggcagttaa acgacaccac aatccgcgtc gctgaccgta tcaagtgcat ccagcccctt
    20761 tccggcgaaa agcccagcgc cttacagctt gcggcccgca gggagaaaga aaagccctct
    20821 atccgggcgc agctcaacgc ggccaagacc gaacaggcca agcagccacc ggcgcgggaa
    20881 aaatcaaagg gattggaggt agagtaaatg cagaagtttg aaaatgtgga cgttctggcc
    20941 gcgctggaac agatcatgcg gcagaacacc gcgttttatc aaagtgactt tggcattgac
    21001 aaagacatta tcaggcggga tgccgccagc gatcaggccg cagacaagac cctgctctgg
    21061 atgtcccggc cctccgggac gtactgtttc cgggaacgtg atgttttcct gaaagacacc
    21121 cgacagtaca acacatggaa attttacggg gagcagaccc gtgacaagat actggcctac
    21181 gcggtggagc tgaccggcac acagggcggc actatccggg gcaatctcta tgagctggac
    21241 tatccgcagc atttccggca cgtcattgaa accgcccagc cggtgagtgt gaaccggctc
    21301 ttttatgagc atggcacccg tgacatcccg gagagccagc gttttgacgg aagccctgac
    21361 cgggtattgg gaaacttttt gtactatgag gcccagcccc atgacccggc tgtgctgcag
    21421 gaggcgctac ggacggagca gcacagccgg gggcgggccg tccccggcga tttcaaagtc
    21481 cacgtcaccg ctttgcatga cagccggatt gaaaccgagg cccggcgtat tgtggacagc
    21541 ctgaaagatc tgtccgcgcc taacagcccc aataaaacgc atttcatggt ggagatctcc
    21601 ccgcactttg cggcgattgc ttccaccaag gataatgacc ggctctttgc catgctgccc
    21661 tataaatccc tgtgttttac cgggatgaaa ggcaggcatg gcgtgtatgc cgtgattaac
    21721 cgggacgagc gccgggatgt gagtattcgc aggccccggc cctctatccg caagcagcta
    21781 tccgacagca aacaggccgc cagcccaaaa aaggcggcgg ccagaaccaa aaagaatgaa
    21841 ttggaggtat aaggaatgat gcactttacc gtggaggaag aaaacctgat ctgtatgtac
    21901 cacaatgcag accggcgcag aacgataaac aggctcaccg ccgtcatgcc ggttatggat
    21961 ggggatatgc gggcgcaggc ccagcagacc ctttccaagc tggaacgcat gacggatgcc
    22021 gactatgacg gccagaagtt ccacttcaca gacgaataac gaacagcaga cgccgggcgc
    22081 ggcggccatg tggtcgcttg cgtccggcgt cttttctttt tgcctattcc tacgcattta
    22141 gaacccggta ttgtcgggga taggatgaag tattcgcttg cccctgcaaa catgcgggag
    22201 aagccttgca gcgggcggtt ttcaatccct taaatgcgta ggttgcagct ataattagac
    22261 ccctgaaaca gatatagccg cgcagcgggt gtatctgttt caggggtgtg caggtgatag
    22321 ccgcaaagcg gcgtaagagg gtgcagccct cttgccgtct atctctgttc cagcgggaag
    22381 ctcggcaatg cttccagcag tttcagggaa atgcgctgcc gtatatcttc gtcaatgact
    22441 ttcttctttt tgccgtttgc ctgtctgatc tccaccgtgg aaagctcgtt gatgtagtca
    22501 gcataatggg ccaacacttt ttccgtggcc cacctctcac cggcaacggc ggctttgatc
    22561 gtttcatagt ccagcagctt ttcttgtccg tccatcctga tacccccgtc agactgattt
    22621 tttctatgat accatattcg gaagcatgga accagagaaa acggcgcttt tctgctggta
    22681 aattctacgc atttagaaca gcacttcggc ggggatagga taaaacaccc acataccccg
    22741 ctgaccatgc cggaaaagcc ttgaaatata agggctttta cagctctaag tgcgtaggtt
    22801 tccagtatct tttccgctgg cgctcggcct ctttgcggcg gcgcatccgt ttggagcagg
    22861 ccgcgcagta tttggcccgg ttagacttcg gcgtgaacgt ggccccgcac tcacagcagc
    22921 gtttcacttc gtcccggtct ttggtgatct cggcatacag ggccgcatcc agcgggagga
    22981 cagcggcctt aaaccagcgg cacagaagcg aataggaaat gctctgtacg caaatacatt
    23041 cttcgccgtc gtccagcaac aggcagttgc cgtcacaata gttgcagcac tcatgcgtca
    23101 gcttccgggc cttgcgatat tgagggtaat tcattcgtgg aatactcata gctcttgcgt
    23161 ccattccttt cccggcgtca tacgcaaaat gccgtccaca ttctgcttga tgatgccgta
    23221 ttcctgcatc tgctttttcg cttcgccgta cttctcatac agccgttcct tttcctcggc
    23281 caatcgcttg tactcggctt tcagggcgta cagatcgggg agctttttca caccctgtcc
    23341 ctttagggcc tttgccgcag cttcatacaa aatcaacgtg ctttcatgct cacgccggaa
    23401 ttttgcctta tccgctgcat tgcggtattc catagccacg ggccagagct gcttgtaggt
    23461 agaaatattc tttatcagca ccgccatatc cgcaagccgg tgttccatag ctttgagggt
    23521 agcggcggcc tcgtcgctgg cggcggtgat ctcggccact ttgctttcca aatccgggta
    23581 ggtgtctatc ccatgttcag tcaggaaatt caaagtcttt gcggcctgct tcaaattgtg
    23641 gagcttcgcc cacctctcat agcctgccga ctgctgggcc ttaatgttgt tttccagatc
    23701 aatccgcaaa gaaatcccct tgcgctcttt gggcgctctg gtgataacgc ggcctttgat
    23761 acgctctttg atggcttcct cggtgtaggc ttcccccaat gtcttgcagc gggtaaagcg
    23821 ttcctgtccg ggagcgcgga aagaaataaa ctttcccggc ttgatctcat agccctgcgc
    23881 ctgcatgagc cgcaggaaat catcaaaatc ttttgcctgc gggatagcgg cgtcaatggc
    23941 tgctttgagc ttcgctttcc agctcgtccc tttccgctgg gcgtcccact cggcatagct
    24001 cttgccctta ctctggccgg gcttcaccac ggacaatccg ttttccttgc acagcctgtc
    24061 gctggtgcgc cggatatagg catagctctg tttgttggaa atgtacttgc gctgatttac
    24121 catatccacg gcacagaaaa taatgtgatt gtggacgtgg cccttatcaa tatgggtagt
    24181 cagcacatag ggatatttcc cttgcagcac ttcctcggca agctgcttcc cgatctcatg
    24241 ggcctgttcc ggcgtggctt cgccgggggc aaaggattgt atcaggtggt ggcccagatt
    24301 attgcccttt tggtatgcct gcgccagcag catttcaaat tcaatgtccg ctgtttcata
    24361 agagcaggca aaggaggcca ccagcagctt gccgtcagtt ttgtccgggt tctcgatgta
    24421 gtcaagggct tttttcagcg ttgacttgat aggatgtatc tttgtaactg ccatatctgc
    24481 gccagcgccc cctttatatc ctcgatgtcc tgtgcgtaca ccggccccat accgttcagc
    24541 ctctttgcaa tctgattgac gttgacgccg atcttctgta tctcggcggt ctgcgcccgg
    24601 atgtcgctgg tgtccatgtt gacgatatag ccgtcaatga gcatcttccg ggcgtaggct
    24661 gaaaagttct tcgtatgcag cagcgccatt ttctgttcaa tcatagcccg ttcctccggc
    24721 gtcacaggga cgcgcagcac gatttttcgc tttctgtttg ccatgcttca ccgtcctttc
    24781 cattcagggg tttggggatt tccccaacaa gcaaaacgga cgcggcccgg cagggacggg
    24841 gcaggccgtt tttccgggag tggctacact ccctatgctt gctacgttac tgtttctcca
    24901 ctaacaagac gtttccaaag ccccaaacta cccgcaaaat agccctctgg aaagaaaaaa
    24961 gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa aagtaaaaca gaggatgcca
    25021 gttacacatc ctccgtttca ttcttcttcg gcttttcaat tagtatggcg ctgtcaatcg
    25081 cgccctcgat gatcggcagg tattcttcgg ggcagagttt cagcttgtgg ctgacccttt
    25141 gccgcagttc gctttcctcc cgcatgatct ccgggttaaa gtagcgttcc acaggaagcc
    25201 cgcaggcttt aataagctgt atgacaaccg gcagactggg aatggcccgt cgttttcgat
    25261 gttggcaaga tagcgccagt caatatttat tttctctgcc attgctttgc gcgaaacgcg
    25321 ctttgcttgc cgggccgctt tcacatccgc gccgaaagtt tcaaagccgg gacaatcttc
    25381 aatattcgcc atatcgcacc tcccacttac attgtatatt tcatactctg cccgtggaat
    25441 gttggtatat gcacctttct taaagtttat atttcacatt ttgtttcttt tattcttcga
    25501 gcaaaagaat tataatgtac cctgtggagg tgtactatgg actacatgac gttgaaagag
    25561 gcaggcgaga aatggggtgt gcccccacga tggataaact actattgtgc tgcgggacgc
    25621 atccccggcg ctgtaaagat ggcgacgatc tggctcattc ccaaaactgc ggaaaagccg
    25681 attgacaggc gtacaaagaa aggaaaggcg gttactgaat agtataatga aaccggcgca
    25741 ttttggatat ggttttgaag agagagaaag catggtattt tgtcacggaa aaactataag
    25801 atgatagatg gcaggctgct ccagaccgat aaaaagtatt ctcatctgaa acagaaagaa
    25861 aagatttaca catggatgta tgaagaagcg aaactttatc atgacagaaa tggaaaatgc
    25921 ccgaaacaaa gacgaggaag ctgctgctgt atcagccgta tatggtagaa ttgaaaaggc
    25981 cggcatctgg attctttatg gtgaagtctc cagacattgg ataactgctg gtctaatgcg
    26041 cctttgagta tggggtgatt gattttaata ccggggaggt acggcttttt gaggaaaata
    26101 ttctgggatt tacatttggc gattattaca tttcctgtaa ctgcaaggac acggatgatg
    26161 aagatatgag gaatatagac ttgctgattt tctactgtct tgaacggtat taggactgaa
    26221 tctgctgcgc ggagtgcggc aagagcgacc ggagggagta cacacaggcc ctgcctgtgt
    26281 gaaaagccat accgcaaggt gcattcgcaa aaaagaggcc gcgcattttg ctgaggcttg
    26341 gggtgtcccc aacaagcaaa acgagaacgc cgcaggcggg caagtggtgg atcagaatat
    26401 ccgtaaagga tattctgatc cattgcttgc cagtattatc gggatgtttt gacaaccgct
    26461 gtaatcgaaa catcccaaac attaagaagg tgttaattat tattatcttg atttaattcg
    26521 tttaaaccaa taatatagat acatacacca tgaaaagaag cgagtatccc aatgacggat
    26581 gattaaaatc cgaaaagaaa tcgctttgaa actacaggga aactacagac tgttatgtta
    26641 tcttcttaaa atggagggat ttttatgtcg atacgaattc tacttgtcga ggatgatgat
    26701 catatctgta atacagtaag ggcgtttctg gctgaggcag gatatcaggt ggatgcctgc
    26761 acagatggaa atgaggcata caccaagttt tacgaaaaca cttatcaact ggttattctt
    26821 gatattatgc tgcccggtat gaacgggcat gaacttttgc gtgaatttcg tgcgaaaaat
    26881 gatactccca ttctgatgat gacagccctg tcggatgacg aaaaccaaat ccgggcgttt
    26941 gatgcagagg cagacgacta tgtaacaaag ccattcaaga tgcagatttt actaaagcgg
    27001 gtggaagccc tgctgcggcg cagcggtgcg ctggcaaagg aaatccgtgt cggcaggctg
    27061 acacttctgc cggaggattt cacggtactt tgtgacggta cagagctgcc cctgacacga
    27121 aaagaatttg aaatcctttt gctgctggtg cagaacaaag gcagaacctt aacgcatgaa
    27181 atcattttgt cacgtatatg gggatatgac tttgagggtg atggcagcac agtccacact
    27241 catatcaaaa atttgcgggc caagctgccg gaaaatatca tcaaaaccat tcgcggtgta
    27301 ggataccgat tggaggaatc ataataatgg aaagaaaagg gattttcatt aaggtttttt
    27361 cctatacgat cattgtcctg ctactgcttg tcggtgtaac agcaacactg tttgcacagc
    27421 aatttgtgtc ttatttcaga gtgatggaat tacagcaaac agtaaaatcc tatcagccat
    27481 tggtggaact gattcagaat agcgataggc ttgatattca agaggtggca gggctgtttc
    27541 actacaataa ccaatccttt gagttttata ttgaagataa agagggaagc gtactctatg
    27601 ccacaccaaa tgccaataca tcaaatagtt ttagacccaa ctttctttat gtggtacata
    27661 gagatgataa tatttcgatt gttgctcaaa gcaaggcagg tgtgggattg ctttatcaag
    27721 ggctgacaat tcggggaatt gttatgattg cgataatggt tgtattcagc cttttatgcg
    27781 cgtatatctt tgcgcggcaa atgacaacgc cgatcaaagc cttagcggac agtgcgaata
    27841 aaatggcaaa cctgaaagat gtaccgccgc cgctggagcg aaaggatgag cttggcgcac
    27901 tggctcatga tatgcattcc atgtatgtca ggctgaaaga aaccatcgca aggctggagg
    27961 atgaaatcgc aagggaacat gagttggagg aaacacagcg atatttcttt gcggcagctt
    28021 ctcatgagct aaaaacgccc atcgcggcta caagcgttct gttggaggga atgcttgaaa
    28081 atatcggtga ctacaaagat cattctaagt atctgcgcga atgcatcaaa atgatggata
    28141 ggcagggcaa aatcatttcc gaaatactgg agcttgtcag cctgaatgat gggagaatcg
    28201 tacccatagc tgaaccgttg gacatagggc gcacggttgc cgagttgctg cccgattttc
    28261 aaaccttggc agaggcaaac aaccagcggt tcgtcacaga tattccagcc gggcaaattg
    28321 tcctgtccga tccgaggctg ctccaaaagg cactatccaa tgtcatattg aatgcagttc
    28381 agaacacgcc gcagggaggc gaggtacgga tatggagtga gcctggtgct gaaaaatgcc
    28441 gcctttttgt tttgaacatg ggcgttcaca ttgatgatac tgcgcttcca aggctgttca
    28501 ccccattcta tcgcattgat caggcgcgaa gcagaaaaag tgggcgaagc ggtttaggac
    28561 ttgccatcgt acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg gaaaacacct
    28621 cggatggcgt tttgttctgg ctggatttac cgctcacatc aacattgtaa atatttaaaa
    28681 cttaaatg
//
LOCUS       DAYYCQ010000031        28426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000031 DAYYCQ010000000
VERSION     DAYYCQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.031"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHAYF_001335"
     CDS             complement(132..509)
                     /locus_tag="ACHAYF_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578783.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHAYF_001336"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHAYF_001336"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8578784.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHAYF_001337"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHAYF_001337"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8578785.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHAYF_001338"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHAYF_001338"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8578786.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHAYF_001339"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHAYF_001339"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8578787.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHAYF_001340"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHAYF_001340"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8578788.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHAYF_001341"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHAYF_001341"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8578789.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHAYF_001342"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHAYF_001342"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8578790.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHAYF_001343"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHAYF_001343"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8578791.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHAYF_001344"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHAYF_001344"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8578792.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHAYF_001345"
     CDS             10661..11575
                     /locus_tag="ACHAYF_001345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8578793.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHAYF_001346"
     CDS             complement(11708..13273)
                     /locus_tag="ACHAYF_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578794.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHAYF_001347"
     CDS             complement(13266..14717)
                     /locus_tag="ACHAYF_001347"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8578795.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHAYF_001348"
     CDS             complement(14731..15747)
                     /locus_tag="ACHAYF_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8578796.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHAYF_001349"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHAYF_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8578797.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHAYF_001350"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHAYF_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8578798.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHAYF_001351"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHAYF_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8578799.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHAYF_001352"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHAYF_001352"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8578800.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHAYF_001353"
     CDS             21340..22764
                     /locus_tag="ACHAYF_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8578801.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACHAYF_001354"
     CDS             complement(22806..23063)
                     /locus_tag="ACHAYF_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578802.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHAYF_001355"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHAYF_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8578803.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACHAYF_001356"
     CDS             24337..25314
                     /locus_tag="ACHAYF_001356"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8578804.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="ACHAYF_001357"
     CDS             25339..26982
                     /locus_tag="ACHAYF_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8578805.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHAYF_001358"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHAYF_001358"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8578806.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT         9234 a         4892 c         6433 g         7867 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaa
//
LOCUS       DAYYCQ010000032        27571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000032 DAYYCQ010000000
VERSION     DAYYCQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.032"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACHAYF_001359"
     CDS             complement(287..649)
                     /locus_tag="ACHAYF_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578807.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACHAYF_001360"
     CDS             complement(723..1103)
                     /locus_tag="ACHAYF_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8578808.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACHAYF_001361"
     CDS             1185..2360
                     /locus_tag="ACHAYF_001361"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8578809.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACHAYF_001362"
     CDS             complement(2437..3021)
                     /locus_tag="ACHAYF_001362"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8578810.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACHAYF_001363"
     CDS             3222..4220
                     /locus_tag="ACHAYF_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8578811.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACHAYF_001364"
     CDS             4332..4841
                     /locus_tag="ACHAYF_001364"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8578812.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ACHAYF_001365"
     CDS             complement(4871..5530)
                     /locus_tag="ACHAYF_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8578813.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ACHAYF_001366"
     CDS             complement(5532..6296)
                     /locus_tag="ACHAYF_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8578814.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ACHAYF_001367"
     CDS             complement(6304..6981)
                     /locus_tag="ACHAYF_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8578815.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVED
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ACHAYF_001368"
     CDS             complement(7018..8403)
                     /locus_tag="ACHAYF_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8578816.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ACHAYF_001369"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHAYF_001369"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACHAYF_001370"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACHAYF_001370"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8578817.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="ACHAYF_001371"
     CDS             9341..10198
                     /locus_tag="ACHAYF_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578818.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="ACHAYF_001372"
     CDS             complement(10279..10614)
                     /locus_tag="ACHAYF_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578819.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="ACHAYF_001373"
     CDS             complement(10681..12051)
                     /locus_tag="ACHAYF_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8578820.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="ACHAYF_001374"
     CDS             complement(12129..12665)
                     /locus_tag="ACHAYF_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8578821.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12974..14455
                     /locus_tag="ACHAYF_001375"
     CDS             12974..14455
                     /locus_tag="ACHAYF_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578822.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14558..15265)
                     /locus_tag="ACHAYF_001376"
     CDS             complement(14558..15265)
                     /locus_tag="ACHAYF_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8578823.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15344..15682)
                     /locus_tag="ACHAYF_001377"
     CDS             complement(15344..15682)
                     /locus_tag="ACHAYF_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8578824.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15793..16755)
                     /locus_tag="ACHAYF_001378"
     CDS             complement(15793..16755)
                     /locus_tag="ACHAYF_001378"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8578825.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16770..18101)
                     /locus_tag="ACHAYF_001379"
     CDS             complement(16770..18101)
                     /locus_tag="ACHAYF_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8578826.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18109..18789)
                     /locus_tag="ACHAYF_001380"
     CDS             complement(18109..18789)
                     /locus_tag="ACHAYF_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8578827.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19008..19487)
                     /locus_tag="ACHAYF_001381"
     CDS             complement(19008..19487)
                     /locus_tag="ACHAYF_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8578828.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19614..21293)
                     /locus_tag="ACHAYF_001382"
     CDS             complement(19614..21293)
                     /locus_tag="ACHAYF_001382"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8578829.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21841..23343
                     /locus_tag="ACHAYF_001383"
     CDS             21841..23343
                     /locus_tag="ACHAYF_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578830.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23356..24018
                     /locus_tag="ACHAYF_001384"
     CDS             23356..24018
                     /locus_tag="ACHAYF_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578831.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24119..24322)
                     /locus_tag="ACHAYF_001385"
     CDS             complement(24119..24322)
                     /locus_tag="ACHAYF_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8578832.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24433..25617)
                     /gene="iadA"
                     /locus_tag="ACHAYF_001386"
     CDS             complement(24433..25617)
                     /gene="iadA"
                     /locus_tag="ACHAYF_001386"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8578833.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25774..27513)
                     /gene="yfcC"
                     /locus_tag="ACHAYF_001387"
     CDS             complement(25774..27513)
                     /gene="yfcC"
                     /locus_tag="ACHAYF_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8578834.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8204 a         5759 c         4668 g         8940 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctt ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga caactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 ctttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatca
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg ctactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa
    12721 aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc
    12781 gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa
    12841 ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac
    12901 acagactgaa atgactttcc actcacagtt tttgtctaac tatatacaaa aacagaataa
    12961 aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt
    13021 cattcaaaaa aataattgtt ctttacaaga attatccaat gacttacata ttccaaaacg
    13081 gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc aagcaatttt tattgattga
    13141 tgaatttatt tattctaatc ataaaggaga gatccaaacc aaagaaaccg atcaaactaa
    13201 aaagttagct gttttttcta agttaaaatt atgctaccta aaagaatcca atcgttttaa
    13261 ctatttgatg ttactaatca attatccaca aactgctgta ccaaaaaaat atttacttga
    13321 acaactttat atatcgccct cctatctcga aaaactgaca aaacagctca atcattcctt
    13381 aataaaattc catttacgga tcctatctgt aaaaaattgc tgtactcttg aaggaaatga
    13441 gttatctatc cgtctctatc tttacttttt tctttcagat acatttggag gtaacgattg
    13501 gccattttcc tcacttgaat taaatgcact aaaaatgaac agtacagatg agcaaataga
    13561 aaaaatttca aaacaagaaa aaaatatcta cttgctgtta actttattca tattacgaaa
    13621 caaacaacag gcatattttc caaaaacttc ttctgaaatc actgaattga tggacttatt
    13681 gcagcataca catgattatt cagaatactt tacttctttt tcagtgagtc gcccttgtga
    13741 agagacagaa acagagagag cctactttaa ttattttttc tattgttttt ttccaaatca
    13801 aatctctttt cgacaaaaac aggagattgg ttttttattt tccacaagca aaaaccttat
    13861 ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa cagtttcctc tcaaccattt
    13921 agaaaataaa ataccaattt atttttattt tcttgtgttg gcagttgttt ttttcgaact
    13981 gttgggtgaa tccagcagta gtttttctac actttgcttt ccagaagcca cgtccaatca
    14041 ttctagttct tttatacagg aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa
    14101 aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac acttactaca
    14161 aacagaaacg aaagagcagt tactcatcca tttgcagata accaaacagt ttacaggaaa
    14221 ttatcatctt caaagccatt tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc
    14281 atattcagaa catgctgatt tgatcattac agatcagcat gagaaaattt cagatgaaac
    14341 ggagactttc tatctggata caacgcacaa tacagtgtta tttggcaagt taataaaagc
    14401 ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa
    14461 ttcttgtcac atcctcttat tccaaataaa tggtattcaa tcagctaact ctcggtatta
    14521 gctcagatca aagcgctgat ttcacaactt ctttatttta atctcttcgg ttgccgccga
    14581 acaataagat caagcgcaac agctgcagga atgtcgaaag cgctgctgca acataagtca
    14641 gtgctgctgc aaataatacg tgtcttgcca tcggtacttc ttcttccgtc agtagtccgc
    14701 cttcactcaa aatttggatt gctcgccgag acgcgttaaa ttcgactggt aatgtcacca
    14761 attggaaaag taatgctaaa gaaaaagcga aaatcccgat attgattaac gtagagttcc
    14821 agctaagcaa tacacctacc ataatcaagg ggaacgaaat catcgaacca aagttagctg
    14881 ctggaacaag tgctgcacga atcttcaatg ggacataacc cgaagcatct tgtaccgcat
    14941 gtccgcattc atgagctgct acacctatcg cagcaactga agtagactgt gcagttgctt
    15001 gagacaaact caacatctta ttacctgaat tgtagttatc tgtgagatct ccggcaattt
    15061 gttgaacacc gacattattt atctgttgac tttgtaatat atattgtgct gcttgtgtac
    15121 cagtcacatt gtttttgctt ctcacttgat cgtattttcg gaaagtacta ttgacgtacg
    15181 ctgacgcaat tccggaaata actaatccaa gaatgaccaa aagaaatgtc ggatctaaaa
    15241 aaccatacat tccataacca tacattttcg tttcactcct tttcgtttac tttattttat
    15301 cattagtagt aggaaaagaa tgtgaaaaga ccttgaatat tctttaattc tttttcacaa
    15361 attccgattt cagtttcatc ggtccaaatc cttctacctt gcaatcgata ttatgatcgc
    15421 cttctactaa acggatgttc ttgacttttg ttccttgttt aatggcacct gacgcacctt
    15481 ttactttcaa gtctttaatc actgtcacgc tatcgccctc taacagacgg tttccatttg
    15541 catctaatac cactaatccg tcttctatga cagtttcttg ttcttctttg ttccattcgt
    15601 tcccgcattc tgggcaaatc aacaagcctc gatcttcata tgtgtactct gagccacatt
    15661 ctgggcaatt tggtaatgtc atcttctttc tcctttaaaa tctttagtga gcactattgt
    15721 aacggaaaaa gaagatcttg aaaagataca tttgtgattt ttactacaaa aggcacgttt
    15781 tcgaacgaag tatcattctg tttttctgct cttatacgat tggcatatat cttggatctc
    15841 tttatagatt tcctctactt ctgctaaatc ttttgcattc gcaccacttg ctaatggatg
    15901 gcctcctcca tgatggcgtt tagccaattc attgatgatc ggccccttag aacgtaaacg
    15961 tacgcggtaa taccctgaag gctgctgaac aaaaatcccc caagccagca cttcgtcaat
    16021 cactccagga agtgaaacga tcgaagctgt ctctgaatca tcgattccat atttatcaag
    16081 aatttcttgc ggaaggatta ttcttcctgc accattttca tccacctgga tattttgata
    16141 aagataaccg gaaagtttag cgactttcat cggcatttgt tcaatctctc tgttcaaagc
    16201 acttgcatca aatggataat ttctcaatat cgaagcaatc tctaatgtac gggacgtcgt
    16261 ggagggatac aaaaagcgcc ctgtatctcc gacgattcct gcataaagta accgtgcagc
    16321 cttaacgttc atctttaaag attcaggaaa catcaaagca aattctgcaa tcatctcact
    16381 acagctactt gcttttgtat ttacccacac aagatctcca taagggtcat cattgggatg
    16441 atggtcgatt ttgatcagtt tttctgccaa ctgatatctt tgatcactga ttctgggtga
    16501 attagctgta tcagtcacga tgactagcgc tccttggtag aagacatctt caacttcttg
    16561 catttctgct aaaaagcgaa gcccttcaat cgtttcacct acttgacgta tttctttttc
    16621 tgggaaactt tcccgtaata gttcggctaa tcctacttgt gatcctaatg catcaggatc
    16681 agggcgcaaa tgcctgtgga taatgatttt atcgtatttt tttattgtat ctactatttc
    16741 ttgctgtact gtcatttcac tcctgctttc taagaacgtt ccattacttg acaaacaacg
    16801 attgctttgg aaacgatggt gttttcaata aacacttcga tatccagttt agcggaacgt
    16861 ctgccgattt ccaatacacg tggacgaatg tctaattcgc tttccaattg gatcagccgc
    16921 aaataatgta ggttcacttg ttcaatcaag atatttcgtt tttggttcat caccattgtt
    16981 ttttgtgcga catcagaaat gatttcgcta agtacgccaa aagaaatggt tccaacgcca
    17041 ttgaccattt gtggtgctac tacgaatttg aacttagggt tttccaaacg atttccttca
    17101 cggtcagtat caatcgtatg gatatttcca gatatctgat ccgaaatcgt atctgcaatc
    17161 tgcggttgtc gctggacaag ctgcatcgct ttcattacat cttgccgggt aatcaagcct
    17221 tccaatgtca agtcatcgga aacgaccggc atgacttcta gcccatccca gatcatttga
    17281 tggctaacag aggcaatact cattgttttc tttacactga ttggttcttt cgtcatcacc
    17341 cgatcgatga tctggtttgg cgatttccct aaaacatctt ttgccgtaac aatccccatc
    17401 aaacgatgat ggcggttgac gactggaaaa cgcgaatgct gcgtttcttc tgacaaacgc
    17461 tgataatcag aaatcgtatc tgtctgctgc aaataatgag tcttttcagc tggcatatag
    17521 atatcactga caagcatgat atcttttttg atcaattggt cgcttaacgc tcggttgatc
    17581 atggttgcga ctgtaaatgt atcataagag gtgcgtaaga tcggcatttc aagatcatca
    17641 gctagctttg caatctcagc agtcgtattg aatcctcctg tgatcaatac tgcggcgcca
    17701 tcttccaatg ctaatttttg gacttccgcc cggttgccga cgatcattag tgatcctggc
    17761 gtaatatagc gttccatggc atgtttagtc atcgcgccga tgacgaattt attcaagacc
    17821 ttgtctaatc ctgccgcccc gcctaaaacg tccccttcaa tgatcctgac gacctcgcca
    17881 aatgtcaaac gttcaatatg tttttttaat ttgcgttcga tacggatggt ccctactcgt
    17941 tgaattgttg aaactagtcc gacattttct gcatctttga ttgcacggta cgctgttcct
    18001 tcactgacat tcaattcttt tgcgatgctt cttacagaaa ttcgattgcc aacaggtaaa
    18061 ctttcaatat attctaaaat caaatcatgt tttgtcgcca ttttctgcct acacttccag
    18121 cgtttccgag acgctcatta tttttccttc actatcttcc agtaactcta caaaagattc
    18181 gggatcttgt tcaatgacag ggaaagtatt gtaatggatt ggaactactt tcttggcacg
    18241 aattgtttgc gcagcctttg ctgcatcttc tggccccatc gtatagttat cgccaatcgg
    18301 caagaaagcg acatcaatat cgaattcttc tgcaagcaat cctagatcac tgaaataagc
    18361 agtatcccct gcatgataaa tcgtttttcc ttctgcttga ataataattc cgctaggctc
    18421 gcccatatac tgaacgatac cgtctacttc atagcttgaa ctatgctgtg catgtacgaa
    18481 ttttaccacg ccgaatggaa acgcatgttt tccaccgata ttcataccga atgaatctac
    18541 tcctctgctg ttcgcataat cagcaatctc aacaatactg ataattgtgg cttgattttc
    18601 ttttgcaata ggaatcatat ctcctatgtg gtcactatga ccatgagtaa tcagcaacca
    18661 atctgtcttt acttcttctg cagttaagtc tgtcaatgga tttcctgtaa taaacgggtc
    18721 aatcatcacg cgttggccat tttccatttc aattgttaaa caggaatgtc catgatacgt
    18781 aattttcata ttttacacct cgcgttttct ctgtatcttt tttattaaaa acgatttcat
    18841 cacttgcaca tcaatgaaaa aatatagctg ctaaatatat tttcattgga ttgatagtac
    18901 agtatgtaca aagcaattgt aacagataat acaggatgca ccaagtacca agcagatata
    18961 atctcaagtt tgaggtctct gctctgctta gcttctcttc tcgtccctta actaattaaa
    19021 tcagcgtaaa taggatggac gatcttttct aaatcggcta cttttatccg aaggcttctc
    19081 ccgatttcac cgcttgacac aattacttct tctttttctt ttaaagaact gtctgctaaa
    19141 aaacaataat caggatgtgc gagttttatt ccaatagggg tgtttgctcc gtggggatat
    19201 cctgtatgca ttaatacaaa atccattcca ggaaaaccaa ctttacgatc tttcgtcact
    19261 tgtctcgttt ttttgtaatc taagtgatcg ttcaaaggaa tcaatgcgat gatcacccca
    19321 gttttgtttc ctttcactac gagagtttta catatcaaag ctggatcaat ccctcttttt
    19381 tgtagttctt ctgtgaaaga ttcttctacc acatgactaa aatcaagtgg ttgatagctg
    19441 atcttttgtt cttctaaata acgttcgact tttgtttgac tcatcattga ttcactccct
    19501 ttattctatc gtttattata aagcgaggga tgctgtattc aaaaattatt tgtttttcgc
    19561 aaaaaaagag caagacgagc actctataga gaggttcatc ttactctttt tgtttatttt
    19621 ttatttcgac gttgagtagc ttccataatg actggcaaga tcactgggcg gcgttttgtt
    19681 tgttcaaata agtaacggcc taattgatct cggatgtctt gctttaattt gctccactca
    19741 aaatcattgc tttctaaatg tttctcaacg attgtttcaa tcatattgct gctttctttc
    19801 atcagatcac ggctagcttt tacgtaaacg aatccccgtg aagtaatttg aggttttgaa
    19861 atgattttct tttctcgccg actgatagtg acaaccgcta cgaaaatccc gtcttctgac
    19921 aagatttttc gatcacgtaa aacaatgttt ccgatatcac ctatgcctaa tccatcaatc
    19981 attacatttt ctgcacttgt agttcctgca acactcatac gcttgttctt atattccagc
    20041 acgtctccgc gtccagtaat atagatattt ttgtatggca tccctactgc atgagccaaa
    20101 tctgcatgag cagctaattc acgatactct ccttgaactg gcacaaaata tgttggtttc
    20161 atcaaattga tcattaattg caagtcatta ggattggcat ggccagaaac gcgcatattc
    20221 tcagagatca acttcactgt tccaccagct cgataaatga tatcttccgt tttcgccacg
    20281 atggtttcca tcgcgatgct tggcgtagta gtgatataaa ccaaatctcc ttcttcaatc
    20341 ttgacattac ggtgtgtacc gtttgccatt ttttgcagag acttgatggg ttcacccatt
    20401 cggccagttt ctaaaatgat cagttcttct ggatcatagt ttttcatgtc tttcaacgta
    20461 acaagcagat cttcacttgg taattgaagt ttttctaaac gcatcgctgt gcgaataatt
    20521 cgttcgaaat cttgaccagt caagacaact tttcgttgtg ctttttctgc tgcatccaat
    20581 acttgttgca ctcgttgcaa attacttgct acacaagcga cgatgatacg cccttcccaa
    20641 tatttgatcg tatcaaatac ttcatctgct atttgggctt cagaagctac aggctgagga
    20701 ttttctgcat ttgccgattc acttagtaag gctaagactc cttctttgcc gatttccgcc
    20761 aatcgtgcat aatctgtttg atacattggg atagccgttt ggtcaaattt aaaatctccc
    20821 gtataaacga tactaccttc ttttgttttc agattgatcc ccactgaatc tggtatcgta
    20881 tgagtggtac ggaaaaagct cactactgtt tcaccaaagt ctatttctgt atgctcatct
    20941 accacatgga aatccttaaa tcctttggct gcttcattac gactgacatt caattttgct
    21001 aactcgatcg tcaactctgt cccgaatact ggtacagaaa tttttgacaa caagtaagga
    21061 agtgccccga tcgcatctgc atgtccatgt gttaaaaaga caccagctac acgatcaatg
    21121 ttttcttcta aatacgtaaa atcagggatt acgacgtcga tccccaacaa ctcattttca
    21181 ggatatttta gcccacaatc taaaataaaa atatcctcgc ccacttctgc gacatacatg
    21241 ttttttccat tttcacgaac gccgcctaat gggataattt taatggtact cacaaacttc
    21301 acctcttctt atgacatcca agtttaaaga tgtcgatttt cttctttatc taaatatatg
    21361 agccattcca atgaagcata tgcaaaatca ctatttctca acttatggag aatcagcagg
    21421 aaaattacca gcacatccgc gttctgacag ttggatgggc attttatttt ggatatactt
    21481 cattaacaaa atggatcttt tgcgaaacgc tatagccaga atacttatat aagtattcag
    21541 ctgttcccca ttattataca acaaataaac taaatcgcta gtatcagcaa ttgttagaac
    21601 aactatgcac gtaaaaatcc ctctgtagca tctaggattt cttttgctat tcctctttca
    21661 tttcttcttc gtcggtttga aacatacttt tttcatttta ccacattttt tgcaataact
    21721 atagtgactt tttatatata cagaacctca tgttgatggt gtgcaaacag cataagattt
    21781 aagctagctt taagataggt tgtctattat caaatgcatc tactaggagg gatgcaaaat
    21841 atgaatttta tgaaacgtgc atggaaaagt acaaaagtaa aatggggacg ttcattgtta
    21901 ttgtttgccg tgttcacagc cattcttatt tttgtgctag ctggcctcac gattcgaagc
    21961 gctgcagaag aagcagcgaa tcaagcacaa aaagaagttg gagcaacagt gactttaagt
    22021 gccaatcgag aagcttcatt taaaaaacag gaacaaactt cttcaagcgg ttctaccact
    22081 gaagaacgtc cagatccagg cagtttttct cttacaccag tttccttaac ggacgcggaa
    22141 aaaatagctg aattagataa tgtaaagagt tattcctttg aaagttccgc ctcagctatg
    22201 gcaaaagatg gaataactgc catcactagt tctgattcaa gtgaagacag ttcagaaact
    22261 actgatgcag gcggtgaacc tgctggagga atgggcggga atccgcaaat gactcaagca
    22321 gactttcaaa tcagtggtgt gtcagcaact gcacaaaata gtaatttttc tgatggaaca
    22381 gcaaaaatca cggatggtga aggaatagat gacagcgaca aaggcacaaa caatgtagtc
    22441 attgattcta ctctagcaga aacaaatgac ctggcagtcg gagatacttt cacaatcacg
    22501 agtactgaag atgaagatac cacttatgaa atgacgatca aaggaattta tgaatccagc
    22561 gaaacaagcg gcagtatggg tatgaaattc aacttcatga atccgtcgaa tacactttac
    22621 acttcttaca cttttgcaaa tgaactaaat gggacatctg acgataacac gatagattct
    22681 gctatttaca ctctatctga tccagataaa atggatgagt ttctcaaaga agctgagaag
    22741 ctgatcgaca cagatacatt tagcttacaa tcaaatgatt ccatgtatca gtcaatgttg
    22801 gaacctttaa acaacgtggc aagtttttct aaaaatgttg ttttactagt ggctgtagcc
    22861 ggtattatca tacttactct gatcattatg attaccattc gggagcgtcg gcatgagtta
    22921 ggcgtcttgc tatctttagg agaatccaga agtaaaatca ttctccaatt gtttaccgaa
    22981 gtggctattt gtatgattct tgcattagga gttgcttcgc ttagcggaaa cgtcgtcgcg
    23041 aatgccgtgg gacagcagct acttgaccaa cagacagaaa ccacgacaca aaaccagcaa
    23101 gctcctggcg gtggacaaat gccaggtaac agacaagaac aaggcggtca acgcgatggt
    23161 caacaaggaa ccacgaataa tccatttgct gtttctgatc aagtcagcga attggaaatc
    23221 aatgtcaaac ctgcacaatt gggactatta gccggagtag catttggaat cagtctgttt
    23281 tctgtctttt tagcttctat agggatatta agattaaacc caagaaaaat cctattaaac
    23341 taaaggagga atttgatgtt acggacagaa caattgggat tttggtatca agatccgcaa
    23401 gatgcgcttt ataaagatgt agaactgaat ttcgaagctg gaaaaatgta cgctatttta
    23461 ggagcaagtg gttctggaaa aacgactttt ctttcgctta tttctggact agatgcacca
    23521 aaagaaggaa aggtatacta tcaagatcgt tcactgaaca aaatcgggtt aagaagttat
    23581 cgtaaaaatg atgtctcgat tattttccaa gcttacaact tgcttcctta tttatcggct
    23641 ctagacaacg tcatagcagc aatggaaatc actaagacca agcaagagaa ttatcgacaa
    23701 gttgctttag atcgactggc tcgtgtagga attgatgaaa tattagcaaa aaaacaggtg
    23761 accaaacttt cgggaggtca gcagcagcga gtcgctatcg ttcgtgctat ctgctgtgaa
    23821 catcaactaa tcgtagcgga tgagcctact ggaaatctag atgaaacaac ttcacaagat
    23881 atcgtcaaac ttttccaaga aatcgcccat gaacaaaatc gctgtattat catcgtgact
    23941 catgagattg aagtagcaaa aagctgtgat gaagtctttg aactaaaaaa taaaaaattt
    24001 caaaaaatcg aagtttaaaa aacaggataa tgatcaaata aaaaatccga accatatctg
    24061 tctcgcagat atggttcgga ttttttattt gcttgattca attagtaata agtggttttt
    24121 attttttttc gtcttttcga gaaaacttgt ctttgacttc gtcgcctaaa tttcgagctt
    24181 tttctttgat gtcagcacct agtgattgtg ctttcccttc tgtttctttg ctcttgtcgt
    24241 ctgttgcttt gccgtatgct tcttttcctt caccgactac tttatctttc atatctttag
    24301 aacgtccttt taaatctgcc attgtgcttc ctcctcttag tttgtttatt gatctacctt
    24361 cagggtaaca tcaacaacag atacagacaa acagaacgct cgttttctaa aaaagatagt
    24421 tagagctgtt gattaaaaac ttttttcgaa agtcccccat actttgactt ctttttcttg
    24481 gaccattatt tctcctttag cgataactgt atcaatatca agcgtttctt tatccaagat
    24541 acacagatct gcatcagcac caatttcgat ttttcctttt ttatccaatt tcaatatacg
    24601 tgctggatta ctggttatag ctcgtaaggc gatttccaaa gggatacttt ctttttgcac
    24661 agcttctttg atccctacta gcaatgcctt ggcacttcct actcctaacc ctaggaaatg
    24721 attgttttca tcaaaataag gcaagcttcc ttgtccatct gaggtcatcg tgaaattatc
    24781 taaagaaaca tcttcctcga tcaatcgttt cagtgctttg ctgaagcgaa cttcaccatc
    24841 tgtcttctcc caaaaatcag gatcttcgct agcagtgaag tcaatcgtgc caccacgttt
    24901 ggcaaatgcc acgcaagctt caaataattc tggtgtgcga tttgcatgag taggcaaaaa
    24961 ttgtgtgatc ggaatttctg tttcttctac gattcgtgtc aatagctcta gctttctttt
    25021 tcctccgcct aagtggacat tgatgatccc agcttttccg gataacatgc ctcctacacg
    25081 agcatctgct actgcacgag cgaactcttc aaaacttggc tgagaagaac ggtgatcgga
    25141 tactgcgatt tctccgattc cgataatttt atcaattgtc aaaaagtctt tgatgataga
    25201 atctgttact gtttttaccg gcaatcggta cgaaccttcg tatacataag tggagattcc
    25261 ttctgcttcc agacctttcg ctttgctgat caacgcagtc atatctctac cttctccgtc
    25321 tgttcccaaa caaccgacaa ctgtcgttac accagctgta gtcaattggc tcaatgtgac
    25381 ttctggcgtt cgattttgat aaccgccttc tcctcctcca cctaaaatat ggaaatgaca
    25441 gtcaatgaaa cccggggtta gtacttttcc ctcacctgaa agttcttcta cttccactcc
    25501 ttcgtagccg ccttctagct gttcttctac agccaaaatc ttcccaccgg caatcagtac
    25561 atccattttt cctaagtaat ctggtgcaaa cacttcaatt tgtcgaatga ttttcataca
    25621 tttttcctct ttctttaagt ataactgatg ataggatctt aaaaaataat ccaaacgata
    25681 ggatctttac tcctttgatt ttaccgtaaa atgacggttc ttggagtcct tatctactgt
    25741 ttggattact tttttattta caggtctcca gtactactgc agacctattg caatggcaat
    25801 catgatgaaa atagttccta aaacaaacag gaatccttgc atcttgattt ggaatcttgc
    25861 ccattttgcc cattcgatcc gtgcaacacc tagcacacca atcaagctgg ctgatacggg
    25921 tgtaaaagcg tccataaaac ctgaaccaag ctgataagca agaactgcta cttgtctgga
    25981 tacacctact aaatctgcca gtggagccat gattggcata gtcaaagctg cttgtcctga
    26041 atttgatgta acaaccaagt tgaacaaact ttggaagatg tacatcgccc aagcaccaat
    26101 gacagctgga acaccggtca acgccgtccc gatcccatgc aaaatcgtat tcaaagctga
    26161 aggaacagat gcatcagatc ctcccaagac aagaagaatc cctttggcca tacctactac
    26221 gattgctgta ccagctaaat cagctgcacc agattggaaa gaagaagcga tgtcattgac
    26281 tttcattcca tctaatttga aaagaacagc aatgattcca gcaactaatc ccatcacaaa
    26341 gaattgagaa gcgatttctg gaatgtaata gcctttttga gtcacgcccc aaacgatcca
    26401 gacaaggaca actaacatct ctaataagat caatttatgc ccaagcatga attttttatt
    26461 ttcatcagat gtctttttga tatgagaacg gaaataatca tctgatttgt aagtcaacga
    26521 actttctggt tttttacgga ttttttctgc atatatcatc atataaccag cggctaatgc
    26581 agtaacaact acccacataa tcaatcggaa tgtcgcacca gacaacacgg ggattccagc
    26641 aatcccttga gccaccgcta cgctaaacgg gctcatccaa gaggtcgcat ttccgacttg
    26701 agatgcgaca taagtcacgg ttactgctac gatcgagtca tatcctaacg caatgacaaa
    26761 tggaaccatc accattgaga atggaattac ctcttctgcc atcccgaatg tagcgccacc
    26821 gaatgaaaaa gcaaagaata atagcggtaa agctaaacgc tccagtccct tagttttact
    26881 gataaacgca tagatacctg cgtcgatcgc tccggtacgc ataataatac caaaagcacc
    26941 tccaacaaca aggatcaatg cagcgatccc tacagcagat ccatacttat ctccagatac
    27001 tagtccttca aaaacaaaat ttaagaaacc aaagccgcca aaatcatctg ttccccatat
    27061 tttggctgtt ttatgcaatt ttttagaagt atcatagata ttttcgccgt actgatcgta
    27121 aagcacatca tctgtcaagg agatttcatc tagtttttcc tgcgtaagat ttttttctcc
    27181 gtcagcaatg actttttcca atcctttttc cggaacatcc agtttttcac gttctgctgg
    27241 atgatcttgc agttcttcta attgatcaaa cacatacgac ttatccagct cgtaagcata
    27301 acggaaggaa tcttgacgta aaacagtacg agtacttgtt tcgccatttg catctttgta
    27361 ttcaatatct tgcgtgctga actttcctgc tggaacgaca aaagtcagta tccatgcaaa
    27421 gatgactaca ccaaaaataa tgacatacgt atggggtgtc gataatttag tgaagtcgcg
    27481 tttcggtttt cctgaagaat ttgttttctt caaatttatt tcttcctttc ttttttcagt
    27541 aattttgatg aataaaaaat ggataatcaa a
//
LOCUS       DAYYCQ010000033        27436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000033 DAYYCQ010000000
VERSION     DAYYCQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.033"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..1426
                     /locus_tag="ACHAYF_001388"
     CDS             257..1426
                     /locus_tag="ACHAYF_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8578835.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1899..2453
                     /locus_tag="ACHAYF_001389"
     CDS             1899..2453
                     /locus_tag="ACHAYF_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8578836.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2626..3063)
                     /locus_tag="ACHAYF_001390"
     CDS             complement(2626..3063)
                     /locus_tag="ACHAYF_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8578837.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3111..4058
                     /locus_tag="ACHAYF_001391"
                     /pseudo
     CDS             3111..4058
                     /locus_tag="ACHAYF_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4329..4764
                     /locus_tag="ACHAYF_001392"
                     /pseudo
     CDS             4329..4764
                     /locus_tag="ACHAYF_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4780..5613
                     /locus_tag="ACHAYF_001393"
     CDS             4780..5613
                     /locus_tag="ACHAYF_001393"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8578838.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5858..6883
                     /locus_tag="ACHAYF_001394"
     CDS             5858..6883
                     /locus_tag="ACHAYF_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8578839.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7021..7146
                     /locus_tag="ACHAYF_001395"
     CDS             7021..7146
                     /locus_tag="ACHAYF_001395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578840.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7171..8286
                     /locus_tag="ACHAYF_001396"
                     /pseudo
     CDS             7171..8286
                     /locus_tag="ACHAYF_001396"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8369..9265
                     /locus_tag="ACHAYF_001397"
                     /pseudo
     CDS             8369..9265
                     /locus_tag="ACHAYF_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9281..10105
                     /locus_tag="ACHAYF_001398"
     CDS             9281..10105
                     /locus_tag="ACHAYF_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578841.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10137..11447
                     /locus_tag="ACHAYF_001399"
     CDS             10137..11447
                     /locus_tag="ACHAYF_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8578842.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWKSMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11595..12854
                     /locus_tag="ACHAYF_001400"
     CDS             11595..12854
                     /locus_tag="ACHAYF_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8578843.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12864..13736
                     /locus_tag="ACHAYF_001401"
     CDS             12864..13736
                     /locus_tag="ACHAYF_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578844.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13748..14578
                     /locus_tag="ACHAYF_001402"
     CDS             13748..14578
                     /locus_tag="ACHAYF_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578845.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14618..16801
                     /locus_tag="ACHAYF_001403"
     CDS             14618..16801
                     /locus_tag="ACHAYF_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8578846.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16862..18082
                     /locus_tag="ACHAYF_001404"
     CDS             16862..18082
                     /locus_tag="ACHAYF_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8578847.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18079..19539
                     /gene="gtfA"
                     /locus_tag="ACHAYF_001405"
     CDS             18079..19539
                     /gene="gtfA"
                     /locus_tag="ACHAYF_001405"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8578848.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19616..20722
                     /locus_tag="ACHAYF_001406"
                     /pseudo
     CDS             19616..20722
                     /locus_tag="ACHAYF_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21044..21253
                     /locus_tag="ACHAYF_001407"
     CDS             21044..21253
                     /locus_tag="ACHAYF_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8578849.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21246..21395
                     /locus_tag="ACHAYF_001408"
     CDS             21246..21395
                     /locus_tag="ACHAYF_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8578850.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21549..22214
                     /locus_tag="ACHAYF_001409"
     CDS             21549..22214
                     /locus_tag="ACHAYF_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8578851.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22498..23583)
                     /gene="xerS"
                     /locus_tag="ACHAYF_001410"
     CDS             complement(22498..23583)
                     /gene="xerS"
                     /locus_tag="ACHAYF_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8578852.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23917..24150
                     /locus_tag="ACHAYF_001411"
     CDS             23917..24150
                     /locus_tag="ACHAYF_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8578853.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24147..24554
                     /locus_tag="ACHAYF_001412"
     CDS             24147..24554
                     /locus_tag="ACHAYF_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8578854.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25691..26209)
                     /locus_tag="ACHAYF_001413"
     CDS             complement(25691..26209)
                     /locus_tag="ACHAYF_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8578855.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26576..26824
                     /locus_tag="ACHAYF_001414"
     CDS             26576..26824
                     /locus_tag="ACHAYF_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578856.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            27327..>27436
                     /locus_tag="ACHAYF_001415"
     CDS             27327..>27436
                     /locus_tag="ACHAYF_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8578857.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         9397 a         4171 c         5067 g         8801 t
ORIGIN      
        1 aaaggtaata agttattctt ttattactat gtttgggttt tatagcaaag aaagtttaat
       61 aagagtttgg acctagcaaa caatattaat gaggtcatta aatcttatta tctttagtga
      121 tataacgaaa tatctacaca gacaaaatga tagcgtaaaa tattttgtgt aaatagatat
      181 ttagggtaga aaaaaagaaa gtccattctg taaaattaaa gttaccacaa ccgaaatttt
      241 ataggaggac tttctcatgc ccaattttac tacagaaatt atggaaacac taatcaataa
      301 aggtgattta gatgaattat ttcgatgtca tttagaatta gctgttaatt cattattgca
      361 ggctgaatta acagcttttc ttgattatga aaaatatgat cgtgctgggt tcaattcagg
      421 taattcccgt aatgggaatt attcacgttc attcagaaca gagtatggag aattaaattt
      481 ggtaattcct agagatagaa atggtaagtt ttctcaacaa actttacctg cttataaaag
      541 aaccaatgac tctttagaaa ccactattat tcagctattt caaaaaggaa ttactatgtc
      601 tgaaatctct gaattgattg aaaaaatgta cgggcatcac tatacgccac aaaccatttc
      661 aaatatgact aaaatagttt ctgaagatat tattgccttt aaagaaaggt ccttagaatc
      721 cagatactct gtcattttta tggatgctac tcacattcct ttaaaaagac aaactgtttc
      781 aaaagaagca gtttatattg taattggcat tcgattagat ggaaccaaag aggttcttgg
      841 gtttagtctt gctccaactg aatctgctta tgtttggaaa gagattcttc aagacctaaa
      901 agaccgtggt ttaaaagagg ttttattagt cgtaactgat ggtttaagtg gcattaacga
      961 tagtattcat agtgtttatc caaatgctca atttcaacaa tgttgtgtcc atatatctag
     1021 aaatatcgct cataaggttc gtgttagtga tcgacaagaa atctgtagcg atttcaaatt
     1081 agtttatcaa gcaagttcaa aagaagaagc aaataatcaa ataaggttta tgatagataa
     1141 atggaaaaag cagtatccac gagtggttaa attgctcatg aatcctgcta tattaacctt
     1201 ttataatttc cctccgtcaa tcagaagaac tatttattca actaatttga ttgaaggttt
     1261 taacaaacaa ttaaagaaat ataccaaaag aaaagagcaa tttcctaatg aagaatctct
     1321 ggagagattt ctagtatctc aattcaacaa ctataatcaa aagtttctat gccgagttca
     1381 taaaggtttt aaagaaatac atgatacatt agaatcaatg ttttaacttg atcaacagaa
     1441 tgggcttttc catttacaca aaattattga cgctcccgac aaaattatga tcttgcgtaa
     1501 agttataaaa tattaataaa aatattttat taatgaaaag cttggtagca cggttattca
     1561 tatgctaatt tatttcactt tatagtaagg ggagttctta gtatattgtt caaacttaaa
     1621 tttcatataa tttaattatg taaattagct attctaagaa cttctacgga taactgcccg
     1681 tctaaaattt aaataagagc ttagaatctt catataagta aggttaattt ttttgtacaa
     1741 aaaagtcgga atattctgag ttactgaaat gatataaaat cataaactaa atcccttgat
     1801 tttattgact ttcaaaagtt tatatatata agttaatgat tatggtaaaa tatatacata
     1861 atcattaact cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt
     1921 taaggacaaa tgaagagata caagattttc agttttggct aagaagaaca agtaatccgg
     1981 aaagagatag ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta
     2041 taggattaaa agttggagat atacgaggca aaagtaagcc tattattgtt gaacataaaa
     2101 caggtaagcg aaaaccgatt tttatagata atttgagaga agaaatttta ttatatactg
     2161 aaggtaaaga agaaaatgat tggttatttc cgagtcggca acaaggtcga catataacaa
     2221 gggatcgtgt ctatcagata tatgcagata tagcagaaaa attaggacga gatgatatag
     2281 gaacacatac attaagaaaa acttttgggt atcactacta caagaaaact agagacattg
     2341 ctaccttaat gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta
     2401 taactgaaga tgaaatagga gcatcacttc gtggatttaa actaggtgta taatttaaaa
     2461 atttttataa acagtaaaga caaagattaa aagctatttt tatctagaac gattttattt
     2521 cataaagttc gcggaataaa agctggggtt actggtactt ggtataggtt cgtgttatat
     2581 aaactaagta attaaataac atttttaggt tggaaaagga tgaaattaaa aaaagagaat
     2641 caatacggtc tcaaaaacgc aaacgctgcg gaataagaaa aatactgata actcaacgtt
     2701 tagcagacta tctaataggg atagtctgct tttttcgttt ttgaccatac ttttgacctt
     2761 ctgcaaactt ccttgctttt cctctgtaga actcatgaaa tctgcaaaac gaattgccgt
     2821 ttcctctgtc tgctttttcg cgatatgggt gtatatatca tccatagtgg ttttaatatc
     2881 tgtatgtcca agtcgaacat acacttcttt gacagacgca ccagattcaa agagcagact
     2941 acaatgggta tgacgaaaac catgaagtgt gatatgtttt aagttattct ctttaatgat
     3001 gattttcaaa ttgtgattta catagtctaa ataaaggggc ttattttctt ctgatgaaaa
     3061 caaaagttgt tcaccatctt ttataggttt gattctcatt tttagatatt atgcccgcac
     3121 gtattcatca catttctatt gtgaatcgct ttattcgacc gacatttgac ttttatcata
     3181 atatactcgg tttaaaatta ctaatgaaaa caattaatca agacgatcat accatgtatc
     3241 acctgttttt ttcagataat caccaccgtg tgggaactga actgactttt tttgaagtac
     3301 aagaagggaa caaccagttt tttggaacca atacgattga acgaacgata ctaaaagttc
     3361 cttccgaagc atcattgcat ttttgggaaa tatattttga aacacaaggc gtttgccatt
     3421 atggaatcga atcattttct ggacgaccta tcttacgttt tgaagggcca gatgcaacac
     3481 gattagcttt agttccctta cgcgaatttg aagatattga tgattatttc ccgtatgtca
     3541 cagaccagat ccctacagag catgccattt taggaattga tgccattcaa ctacgcgtac
     3601 aatattccga tgcgactgaa cgagagttat tgtcggtagg ctggtagcac aaagaatacg
     3661 tcagtttctt ttcgggtcat caacgagtga caatcttaga aaatcaagac actcagttct
     3721 atcaagaagt tcagattatt caagatcgtg agaatccagt tgccattgaa gggattggta
     3781 gcgtccatca tgttgcgttt ggcgtagaaa ataaatctga tttacaacgc atcgacaaac
     3841 aattacaaga gcgcaatttt ataaattcag gaataaaaga tcgtgaattt tttgtatcac
     3901 tttattatcg tgacccaaat caattattga ttgaaattgc tacaggggaa ggaaacctag
     3961 atgcaaaagc gtatgaaaat caaagtccac gctttgaaga aattccttta tttttaccgc
     4021 agtacttaaa tttcattcgt gaacaagtgg agcaataatt agctaatcaa atccatgacg
     4081 aagagtaaga ggttgactca taagtcaaaa aataaagaaa aacgcatcaa atccattcta
     4141 aagaatgttg atttgatgcg gttcctattt ttataaacta tcattttcga ctaataaaac
     4201 tacttatgag tcagcctctc tttttttctt gatttttata cctctttcta ggtaaactaa
     4261 acatctgcca aaaataccgt gtctcattga aacattctca actatttcaa aataaagggg
     4321 agtaccaaat gaacgagtat attgaaatta tccacgcaag agaaacaact taaaaatgtc
     4381 agtctcaaaa ttcccaaaaa taaaattagt atttttacca gcgtatctgg ttcaggaaaa
     4441 tcatcgattg tttttgatac tattacccaa gaagctggtc gtcaactgaa tgacacctat
     4501 gatagtttta cccgtttatt tttacctaaa tacagtcgtc cagaggttga tgagattgat
     4561 aatctttcta cggcgattgt ggtcaatcaa aagaagattg gtggcagtgc gcgctcaacg
     4621 ttagggacga ttactaacat caatgcgtta tttcgtgtgt tgttttcatg ttttgcaaca
     4681 cctagtttag gatatgcaaa tgcttattca tttaatgatc ctagtggaat gtgttctgtc
     4741 tgtcagggaa tcggtaagac gattgcattt ttaataggat tgctttattt ttcggctatt
     4801 gataatgctg attttgtatt ttctgatcca tttaccgcag aagttttgtc tggaattgag
     4861 gaacaggcaa agaatcatgg ttattttgta ttggtgcata atgttaattc agcagaagat
     4921 gttaaaacta ttcagagaaa ttggcgtttt gaggggttca ttgttgttgg tgtacgtgta
     4981 aaagaatttt tggaattaaa taacgttttt gatggtcctg ttgtttatat tgatacgcat
     5041 ctttcaaaag agacattaag agaacttgaa caacaaatga atcgtttatt tgtaaataca
     5101 aatgattacc aagctagtac attggcagtg gaacatttga ttcattatgg tcatgagaaa
     5161 atagcgtttc tttcttttga ctacattgta ggtgaaccaa gtgttattca agaacgacat
     5221 caagcttata tcgatacatt aaagaaatat catttaacct ttaaatcgca ttggatatat
     5281 aacaactctg aatttgagcg aatttatcaa gaattagctg aatttactgc aattgttgtt
     5341 actggagatt atttagcggc taaattagta aaatttttaa aaaataagaa taaacgagtg
     5401 atggaacaat tatcgattat tggatttgat gatattactt ttgcagaatt aatggatcct
     5461 gcgttgacaa caatacgact aaatccaggt aacaaggggg cgattgcttt tcaccaactg
     5521 attggactgg ccaaacaaga agaaataaaa aatcaagtac tactattaga gggagaattg
     5581 atttctcgag actctgttca caaaatcaaa taaaaaagct ctgtgaaata agtatagtta
     5641 acaataaaat atccgaacga tagagagaat gaactgtgat ctagcagttc ctcgtcgcaa
     5701 attcactaga attagcatta tttactctaa attgttcgga tattttttct tgtttttaat
     5761 tatgattaca gtgcctattt ttaacgaaat ttgcttattt tcaaaatgta tgattatatg
     5821 ttagtaaaca ctttcaaata tgaaaagagg agctattatg gtaggaatta gggatgttgc
     5881 aaaacgggcg ggtgtgtcca ttgcaacagt atcgcgcgtg ctaaatgagg atccgacact
     5941 ttcagtaacc gatgaaacca aacgaaagat tgcagaatcg gttaattttt atcattatgt
     6001 caaaaaaagt cccgttgcaa aaaatttaac aaatattgca ttaataacga cagtttctga
     6061 agtagatgag ttggaagatc cctattttcg aacgattcgc agagggattc aagtcgaagc
     6121 agaacaaagc aaagtcaatt tgaagaaaat tattcgtttg tcagaaacac cgttggagca
     6181 agacgatttg ctaaaatgtc aaggtgtgtt gattattggg caggtgctat cttctgtgat
     6241 tgaagaagta tcagcaatta attcgaatgt cgtagtgatt gatgacccta gtattgaagc
     6301 tgaagtagat gcagtttata ctgatttaac aaaagcaacg aatcagcatt tagatcgatt
     6361 gtataaaaaa ggacaccgaa acattgcgtt tatcggagga ggacgtgttc gtttagatag
     6421 ccaaggaaat aaacacgtgt cagatgatga ttatcgaaaa atagcctatg agacgtggat
     6481 gaaacaaaaa ggattagaaa aatatagtca aatttattta gaaggctgga ccacacttga
     6541 cggaatgcag gcagcagatt tacttttgaa aaaacatgct aaagataagt tgccgactgc
     6601 tatcatggta ggaaatgatc ctattgctgt gggggtttat cgttcacttc aaaaaaatgg
     6661 tctacatatt ccagaagata tttcgattgt gagttttgat aatattgaag ttgctgaatt
     6721 tttaacacct gcattaaata cggtgaatgt gaatacagaa gaaattggaa aactagcagt
     6781 tcgcatggtt aatgaacgaa taaaagaagt tagaaatgtt tcgatcaaag tgattgtttc
     6841 taataatatt attgttagag aaagtgaaaa aaatatcgat taaaaaagta aacatccaaa
     6901 aaagggtgtt tacttttttg tttactaata tttttagtaa caaatctcga caaaacatta
     6961 agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat tcaaaacagg aggaattaag
     7021 atgaaacatt tcaaaaaaat agctttaaca gggatagctg ttttaactgg gacattactc
     7081 gcagcatgtg gtgggggaga tagtaattct aaagacgggc aagtaacttt aagctatgcg
     7141 agttgagata ctgaccaagc tccaggacta agaaaaatgt tagatgaatt tgaaaaacaa
     7201 aatcctgata tcaaagtaga aatggagaca acgccttggg atcaatattg ggttaaatta
     7261 gaagctgcaa caacgggagg taatatgcca gatgtagtaa ccatgcactc tagcgaatcc
     7321 tataaatata tgtcacagaa tgctttgatg ggacttgatg atgttatcaa agaaaatgat
     7381 attgacatgg gaaactttac tgaaggaatt gctgatttct atacgcttga agacgaacta
     7441 tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt acaacaaaga attatttgat
     7501 aatgcaggtg tcgattatcc agatgaaaca tggacatggg aaacactcag acaagctgca
     7561 attgatttaa cagatgaatc aaaaggcatc tatggttttg catcacatat tggaacagag
     7621 actggctact ggccatttat ttatcaaaat gggggagaag tatttgccga tgacaataca
     7681 aaatcaggtt tgaatacccc agaagcacaa gaagctttag ctttttatcg agatattgca
     7741 cttgttgatc atgcttcacc aacaattaca gaattacaag atcctgataa gttaagtcgt
     7801 tttcaatcag gtagattagc aatgatgatt gaaggaaact ggtttgcacc gggatttttg
     7861 aaaaatgact atctagttga aaatgttgct tctgccgtgt tacctaaggg aaaagttaat
     7921 gcaacaatta caaatggact tggttgggct gccagtgcga gtacaaaata cccagaagaa
     7981 gtgaaaaaac taatggcatt tttagctagt gaggacgcca ataaaatcca agcggaaacg
     8041 ggggcatcaa tcccagcact aaaaggcttt gaagacgagt gggctgcttc ttcagataca
     8101 attgatttga gtgcttttgg aaaaatgttg gagtatggag tccctcgtcc atacaataaa
     8161 gatggattac gagctgaaca agcagaagtt caaattatta accgtattct gtctggtgaa
     8221 gtaactgttg aagatggaac cgctcaaatt gaaaaagaag tcaatgcgat attgagcgga
     8281 aattaaatta gttaattata caatatacag gcgaaaggat tctatttcgt ctgtatatta
     8341 atggatgaat accaaaaagg gggatattat gaaaaatcga atttttggaa aatatcgtaa
     8401 agatcaagtg atatggggct atatttttat ttttccaacg gcattaggat taattttatt
     8461 aaatatatgg ccagcaattc aaacagcaat tttaagtttt caaaaaacgg tagggttcgg
     8521 tgatactgaa tgggtcggat taagaaatta ccaaaaaatg tttgcagacg gtgaagtttt
     8581 tcagtcttta ttaaacacat taatttatgc agtggtttcg atacctagta ttgttgtatt
     8641 atccttgttg gtggcagttt taatgaatag aaaagttaaa ggattgtcga tttatcggac
     8701 aatttatttt ctaccagtcg ttgctgcacc atccgcagtc gcaatgattt ggcgctggat
     8761 gttcaataat gattatggac tgattaataa catgttggct aaaataggtc tcgatggacc
     8821 agcatggtta acggatccat cgattgcgat ttattctatt atcattgtag gagtttggag
     8881 tgctattggt tataacatgg tgttattact agccggctta taagaaattc ctaaagatta
     8941 ttatgaagca gcggaaattg atggtgctag tccaatccag cagtttttca acattacgtt
     9001 accattagtt acaccaacat tattttttgt agttgtaaca acagttatca atgctttcca
     9061 agtatttgat gttattttta tgatgattac tcctaatagt accgccatgt ttaaatcaca
     9121 atcattagcc tatttattct ataaacattc atttatttta aatgacaagg gatatggttc
     9181 ggcaatcgtt atgttccttt tagtgattat attaattatt actgcaattc aaatgaagtt
     9241 gcagaaaaag tgggtaaact attaaggagg ctatcaaatt atgaaaaata aatttttttc
     9301 aaatgcgcca atccatatca tgcttatttt aggtggtatt gcaatggcat taccttttat
     9361 ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct acccaaattc cacctacaat
     9421 tttccctgag aaatttcagc taagtaacta tcaacaagta cttgatttat taccattcat
     9481 ggctttttat atcaatacat ttttaatggt tgttggacga gtgattggct cggttttatt
     9541 ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt ccagggaaaa atatcctttt
     9601 cagcttagta cttattcaaa tgatgattcc cggttccatt tttattactc cacagtattt
     9661 attagcacaa aaattgggta ttttaaatac aattccagca ttaatattac caggggtagt
     9721 tagtgcgttt ggtacgtttt tattaagaca atttttctta ggtttacctg atgaattgga
     9781 ggaagcggcc tatttagaag gtgccaatat ttggacgatc tttttcaaaa ttatgttgcc
     9841 gcttgctaaa tcaggattgg ttgcacttgg aatattcaca gcgttgtttg catttaaaga
     9901 tttaatgtgg ccattgattg tcaatacttc aattgataag atgcctttag ctccgggatt
     9961 agcaaactta caaggacaat atgccacgaa cttcccacaa ttaatggcag gttcgttatt
    10021 agcaatttgg ccaatagttg tgatattcat cgttttccaa agaaaattca ttgaaggaat
    10081 tgctacaaca ggtagtaaat tataaaacta ttaattagtg attggagagt aagtacatga
    10141 caaaaattac atttattggt gcaggaagta ctatttttgc aaaaaacgta ttaggcgatg
    10201 cgatgttaac tccaagtcta caagatgcac atattgcatt gtacgatatt gatgaaaaac
    10261 gactaaaaga atcaaattta atgcttaaaa atattaataa aaattcgaat gaaaatcgtg
    10321 ctgatattgt ttcttattat gatcgcaaag aagcgttaag agaggctgat tttgttatca
    10381 atgctattca agtgggtggt tataaacctt caacagtgat tgattttgat attcctaaaa
    10441 aatatgggtt gcgacaaact attggtgaca ccatggggat tggtgggatt tttagaaccc
    10501 tacggacact accagtaatg ttagactttg ctaaagaaat ggaagaagtt tgtccaaatg
    10561 cttggttatt gaactataca aatccgatgg cgatgttaac catgggtgtt ttaaaagcga
    10621 cgagtattaa gactgttggt ttatgtcata gcgttcaagt atgcgtacca gaattgtttg
    10681 agcatttagg aatagccgat caatatgact taaatgactt tcaatggaaa attgcaggca
    10741 ttaatcatat ggcattcttg ttggaaatta caaaaaatgg ggaagatttc tatccaacaa
    10801 ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga ttctgtacgt tttgaattgt
    10861 tgaaacgttt tggttactat attacagaat caagtgaaca caatgcagaa taccatccat
    10921 actttattaa atcaaaatac ccagaattga ttgacgaatt agggattccg ttggatgaat
    10981 acttacgtcg ttgtgaacaa caaattaatg attggaaaag catgcgagag gacattgtaa
    11041 ataatgggga gttaacacat acgagaagcc gtgaatatgc ttcatatatc atggattcta
    11101 ttacgacagg aactccgtca gtgattgcag gaaatgtctt aaataaaggc ttaatcacaa
    11161 acttaccgac agatgtttgt gtggaagtac cttgtttagt cgataaaaat ggcgtgcaac
    11221 caacctatgt cggtgaattg ccaacacaat tagctgcttt aaatcggacg aatatcaatg
    11281 ttcaagaact gacagtagaa gccgctttaa cactagataa atcaaaaatt tatcaagcag
    11341 cgtatttaga tccacatcta tcatctgaat tgtctttaac agatatcaaa aatttagtgg
    11401 atgatttaat cgaagcacat ggggactatt tacctgaata taattaaaaa gaagctgaaa
    11461 gagtaaggaa ttttcaattc cttactcttt tcttagttaa atttagtaaa aatattgaca
    11521 aaacacttag taaacagtta cattagtgtt aacaaaaaga aaacgcattc aaattgtata
    11581 aggaggagca taaaatgaag tggtctaaaa aaattatttt agcttgtata gcactaggag
    11641 caacgatctc tttgactgct tgtggaaaaa atcaggaaga tgcaagcggt aaggtagaaa
    11701 tagagttttt taatcaaaaa aaagaaatga ctcaaacgat tcaggagatt gctaaagatt
    11761 ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga tgtaccgaat gctggtgaag
    11821 taattaaaac acgtatgctt gcaggcgatg taccagatgt cattaatctt tatcctcaaa
    11881 gtatagaatt gaaagaatgg gcgaaagctg gttatttaga ggacttaacc gaagaaccgt
    11941 atttagagaa tatcaaaaat ggatatgcac aacgttttgc tattgaggat cgtgtatata
    12001 gcataccatt aaccgctaat gtctatggtt tttactataa caaaactgct tttgaggaaa
    12061 tggggattca agcaccagaa acttgggcag aatttgaaaa aatagtagct gagattaaag
    12121 atcaaaacaa agtaccgttt gcgattgcag gagcagaagg ctggacttta aatggttatc
    12181 accaactagc tatagcgact gctgctggtg gtgaagagga agcgaacaat gtttggcgtt
    12241 tctcagaagt taatggaatt aatgctgaaa gcaaagaaat gcaaaaagat tttaatcgat
    12301 tagatttgtt aagagaacca aaagctttgc aaaacaactg gcaaggtgcc ggttataacg
    12361 atacagtagt tacttttaca aaaggtgatg cgttgattat gccgaatggt tcatgggcaa
    12421 tgccgatgat tcattcacaa aatccagatt ttgaagtagg aacttttcct tttccagcag
    12481 atgaagcagg tcaaagtcta acgattggcg caggagacct agccttatca gtttctgcaa
    12541 cttctcaaca taaagaagaa gccaaacgtt ttgtagaata tatgacaaca cctgaagcga
    12601 tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt tgagggcgtt atcgagaata
    12661 ctcaggattc accattaagt ggtttaactg aacttgcttt tactgatcgt catttagttt
    12721 ggttagccaa agattggaat agtgaaaatg atttttatac cttaacaaca aattatttac
    12781 acaatggcaa tcaaaaagcc atgattaacg cattaaatgc tttctttaat ccaatgaaag
    12841 ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat tttaaatcgt tactgggcaa
    12901 tcatttttgt agcaattcca attattttgc agatgatttt tttctacttt ccaatggttc
    12961 aaggtgcttt ttacagttta actaattgga ctggtctgac ttataatttt gattttgtgg
    13021 gtttgaacaa ttataagatt cttttttctg atccgaagtt tatcaaatcg attggcttca
    13081 ctttaattgt gacgctttgc atgattgctg gacaaattgg ttttggtatt ttgattgcgc
    13141 gggcgttgaa cgcaaatatt aaagggaaaa cattctttag agcatggttc ttttttccag
    13201 cggtcttatc gggcgtggtt gtttctttag tatttaaaca aatttttaac tatggtttac
    13261 caaaaattgg tagtatgtta ggcattgaat ttttacaaac cagtctttta ggaacaacat
    13321 ggggagcgat ttttgcagta atttttgttt tactttggca aggaattgcc atgccagtca
    13381 tcattttctt agcgggtttt caaagtattc cacaagaaat tattgaagct gctagaatgg
    13441 atggggcgaa tgataagcaa attttttgga atattgaaat cacctactta ttaccaagta
    13501 tttcaatggt atttattatg gcattaaaag gtggcttaac agcatttgat caaatttttg
    13561 ctttaactgg tggtggacca agcgatgcca caacatcttt ggggttactt gtttataatt
    13621 atgcttttaa aaacaatcag tttggttatg cgaatgctat tgctttagtc ttatttatct
    13681 taattgggat tgtttcactg gcacaattaa aattatcgaa aaaatttgaa atctaggagg
    13741 tttcccaatg aaaacatcaa aagaaaaaaa tatttctaaa tacttgcttt tattattagg
    13801 gggcttcatg attttagttc ccttagtttc aacgctattc ggttcattta aaacaacacc
    13861 cgatattatg aataatttct ttcgttttcc taatccagcg actttggaaa atttccgaag
    13921 attgttagcg gatggaattg gtcattactt ttggaattcg gcaatcatca caatcattgc
    13981 agtagttgct gtgacagcaa ttattcctat ggctgcttat tcactagcaa gaaaaatgac
    14041 tcgtgggaaa gcatacggat tgatgtattt atttttaatt ttaggaattt ttgttccttt
    14101 ccaagtaatt atgattccga ttaccgttat gatgagtaaa attggtttag caaatatgtg
    14161 gggattaatc ttactctatc tcacttatgc cgtaccacag acactatttt tatatgttgg
    14221 ttttattaaa ctaaatgtgc ctatcagtct ggatgaggct gctgaaattg atggggctag
    14281 tcactttaca acttatttca aaattgtttt cccaatgtta aaaccaatgc atgcgacgac
    14341 tattattctt aatgctctat ggttttggaa tgatttcatg ctaccattat taattttaaa
    14401 tcgtgattct aatatgtgga cactaccatt gtttcaatat aattatacgg gacaatattt
    14461 taacgactat ggtccaagtt ttgcttccta tacagtagga attgtcacga ttacactagt
    14521 ttatcttgct ttccaaagaa gtattatagc tggaatgagc aatggagcag tgaaataacc
    14581 aataaattat tctcattata aaggagttca taaacttatg attatatttg acaatcacac
    14641 acaaacattt catttacaaa ataaaaaaat tagctatcta ttaggaatcg aagaaaataa
    14701 ttatttaacg catctttatt ttggaaaaaa gattacgcac tattctggtg ggtatcgcta
    14761 cccccgaaca gatcgttcct tctcacccaa caatgcaaat gcgcaagatc gtttattatc
    14821 tttagacaca ttaccattag aatttccagg atatggacat ggtgatttta gagaaccggc
    14881 gcataatatt aaattaggaa acggtagtcg tgtaaatgat tttcgttatg atagctacga
    14941 aattcaaaaa ggaaagccca gcttagcggg attgccagcc ttatatacgg aagatgatga
    15001 tgaagctgaa acgctgatta ttcaattgaa agacgcgatt agtggattga ctttacagtt
    15061 gttctacaca atttatcaaa atcacagtgt aattattcgt tctacgcaat tagtaaataa
    15121 tagtgatgaa acagtggaga ttaaccgctt agctagtcaa tcaattgatt ttccaaatcg
    15181 tccactcgag ttaattcatt taaatggtac ctgggcaaga gaacgtcaaa tgactagaga
    15241 aaaaattcat acagggataa aagtattaga tagtaaacgc ggatcaagca gccatcaaca
    15301 aaatccgttt gttgctttag tagatgccaa aacagatgaa tttcaaggag aggcatatgg
    15361 atttaattta gtttatagcg gtaatcacga aacggtgatt caaaaagatc catttgacca
    15421 aacacgggtc attatgggaa ttaattcatt taattttggt tggcaactaa aaactggcga
    15481 ctcatttcaa acgccagaag tcgtgatggt ttatagtaat gaaggtctga atacgttatc
    15541 acagacgtat catgaattgt ataatcaccg tttaattcgt ggaaactatc gtttaaaaga
    15601 acgtcccacg ttaatcaaca actgggaagc aacgtatttt gattttaacg aagaaaaaat
    15661 catgaatatt gtcgatgatg cgaagaactt gggcattgaa atgtttgttt tagatgatgg
    15721 ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt gattgggttg aaacagaagg
    15781 caaacttgct tgtggtttgg aaaatttagc caagcaagtg catgataacg ggatgaaatt
    15841 tgggatttgg tttgaaccgg aaatgatttc tgaagatagt gaactatttc gagcacatcc
    15901 agattgggca ttgggtattc caggtcgtga ccgctcaatt agtcgaaatc aatatgttct
    15961 tgatttttca agacaagatg ttcgtgataa tatctatcaa caaatgacgg cgatttttga
    16021 tcgtgtaccg attgattatg tgaagtggga tatgaatcga aatatgacag aagtctattc
    16081 tgtcttgttg gactcaacga tgcaaggtga agtggctcat cgttatatgt tagggttata
    16141 tgaattttta gaaaagttaa caacgaatta tccaactatt ttatttgaaa gttgttcggg
    16201 tggaggcggt cgttttgatg caggtttctt gtattacatg ccacaaactt ggacgagcga
    16261 caataccgat gccgtggctc gtctgaaaat tcaatatgga accagcttag tgtatccaat
    16321 ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat caaacaggaa gagaaacgag
    16381 tttaactatt cgtggcaatg ctgcgatggc tggtgttttt ggttatgagc tagatctaac
    16441 gcaattaact tctgaagaaa aagcagaaat taaagaacaa gttgcctttt ataaaaagca
    16501 tcgccaactt ttgcaatatg gaacttttta tcgcttggaa agtccgtttg aaggcaatga
    16561 tactgcatgg atgtttgtct caaaagacca aacagaagcc atggtctttt atttccgagt
    16621 attagcagaa gctgcgcatc cattagtcac attgaaatta gcaggattag cagaagaagc
    16681 aacttatcaa atggaagatg taacatttgc tggcgatgaa ttgatgaatc tcggttttta
    16741 tattgactca gaactacatg gtgattatgc gacacaacgc ttttatttga aacagatata
    16801 gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga ctcattccaa acgaagaaaa
    16861 tatgttttat ttacacgcat ttactaaaaa gcagcctgat ttgaattggg aaaatgaaga
    16921 agtacgtgaa gaaatctatg caatgattaa ctattggtta gataaaggac taggcggatt
    16981 tagaatcgat gcgatattaa acatcaaaaa aaaactagag tatggcatct ttgaatctga
    17041 tggagaagat gggttagcct tcattgggcc ctggattctt aatcagtcgg gcattgaagt
    17101 gtggttaaaa gaaatgagag agcggacatt taaaccacat aatagtatga cggtagcaga
    17161 agccgatgtt cctaatgaac gtttagatga atatattggt gaagatggct attattcgat
    17221 ggtttttgat tttagttata cggatattga tgtacctgaa actggtgaat ggtttaaaga
    17281 tagccaatgg acatggacgg atatgcgtac caatattttt acgaatcaat tagttacgca
    17341 agataaaggt tggggcgcct tatatttaga aaaccacgat caaccacgtt caattaataa
    17401 atatattcca gaagaatgga tcaatgatta tagtaaaaaa atgttggcga cgctttttat
    17461 gttactaaga ggtacccctt ttatttatca aggacaagaa ctaggaatga ccaacataga
    17521 aatggcatca ttagaagatt ttgatgatgt agctacgcat tctcagtata aacgggcatt
    17581 agaatatgga ttggcaccag agcaagcatt gaaagctgtc aataaacgaa gtcgggataa
    17641 ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc agattctcaa cttctgataa
    17701 tgtatggtta aaagtaaatc ctaattatcc agaactcaat gcagaagctc agttaaccaa
    17761 tcaacagtcg gtattcaatt attatcatca attaattaaa ttaagaaaaa atagtgtata
    17821 caaagacgtg ctaatctacg gacaatttct accagtgaaa gataaaaatg aacatatttt
    17881 gctttatgaa cgccaattag aagataaaat cgtattagtt atcttaaacg ttacagcaac
    17941 agaacaagaa tattatgtag atgaacagta ccaacaagta ttaatcaata attatgaaga
    18001 tgttttatta gatcaaagtc gaaaattgcg tttacgaccg tttgaaagta ttgtattagc
    18061 taattatgga ggacaattat gaaaattaaa aacgaagcaa tgctcatcac ctatgcagat
    18121 agtttaggaa aaaacatggg cgaattaaaa caagtcttag acaaacattt acaaggtgtt
    18181 attggtggtg tacatctgtt gccttttttt ccatcgacag gtgatcgagg ctttgcgccg
    18241 agtgattata cgacagttga tccagctctt ggaacatggg aagatgtgga agcattaggt
    18301 gaagattatt atttgatgtt tgattttatg attaatcaca tttctcgtga atcaaaattt
    18361 ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca aagaaatgtt tatccgtatt
    18421 catgaatttt tcccagaaaa tcgaccaaca caagaagata ttgatttaat ctacaagcgt
    18481 aaagacaaag cgccgtttca aacggtagaa tttgcggatg gtacgacaga agaggtttgg
    18541 aatacctttg gggaagaaca aattgattta gatgtgacca aagaagttgt gaaagaattt
    18601 attcgagaaa ccatcaaaga tatggcgaac catggctgtt ctttaattcg attagatgcg
    18661 tttgcttatg cgattaaaaa attagatacc aatgatttct tcgtggaacc agatatttgg
    18721 gaacttttag acgaagtgcg tcaagaagca gcgaaatatg acgtcgaatt attaccagaa
    18781 atccatgagc attattccat tcaaatgaaa attgccaatc atgattatta tatctatgac
    18841 ttcgccttgc caatggtcac tctgtattca ctttatagtg gaaaatcaga acgtttagcc
    18901 aattggttga aaatgagtcc gatgaagcag tttacaacat tggatacgca tgatggaatt
    18961 ggtgttgtgg acgctcgtga tttattaaca gatgaagaat tggattacac ctctgaggaa
    19021 ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa gtgccaatta caataattta
    19081 gatatttatc aaattaacag cacctattat agtgcattag gcgataatga ccgtagctat
    19141 ctgttagcac gtgcgattca agtatttgcg ccagggattc cacaaattta ttacgttggg
    19201 ttattagcag gaaaaaatga tattgaactg ttggaagcaa cgaaagaagg gcgtaatatt
    19261 aatcgtcatt actatgattt agccgaaatt gaagaagaag tgcaacggcc agtgattcaa
    19321 caattatttg cacttttaaa atttagaaat gaatctgcgg cgtttgattt ggatggaacg
    19381 attgaagtag caacgccatc agaatcagaa atagtgatta cacgtaaaaa taaaaacggt
    19441 agtgatagtg caacattaac ggctaacttg gatacgaaag aatttatcat tgagtctaaa
    19501 gatataaaaa ttttcactag tgttgcatta aaattttaac ctattccata gaacaaaaaa
    19561 tcctttatac tagcacttgg atgacttatc caagaccaat aaaaaggact catacatgga
    19621 taagtataaa acaaatacag catttaataa atggttttct gccataaaat taggaaaact
    19681 ttctgtcaac tcccaaaaat gtatttttga ttacaattcg tatcgtaaaa aactgtcttt
    19741 tgaagctgtg ctcaagctct ttctctacgc agttaatgaa gaaaaagaaa gtttgcgtga
    19801 tttgagtaca gctcttatcg accgatcgct ccaactagaa accgatgtgt catcgattag
    19861 tcatacccaa ttgtctcgag catttaatgc acttgaacct aaagtgttag aagaaatttt
    19921 tcaacaactg ttagaaaaag ttcaacatca aatcaaacca accaagcgaa atcatctgta
    19981 tctgattgat tccagtacgt tttcgcttag tttaaaacgt cataagtggg cgactttccg
    20041 tcaaacaaaa tcaggtgtca agttacactt aaactattgt tatatggacg atactacgat
    20101 gtatccaact gattttacct taacaaatgc ccatgaacat gatctcaatc aactaccagt
    20161 attggtgaat caaccagagg caacttacgt gtttgatcgt gggtattagg actttgaaaa
    20221 gatggatcag atgcattggg atggctattt cttcgtcact cggattaaaa aaaacaccaa
    20281 agttcacgtg atagatactc tggaaacatc gccagaaaca gaaattctac gagacgagct
    20341 ggttcgttta ggatctaaaa cctatctaac cgccaatttc cgactagtca ctgttcaaga
    20401 taagaacgga cgtgtgtttc aatttatcac caatcgaatg gacgtctctt caaaagaaat
    20461 ctctgatatg tatcacgctc gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca
    20521 catgaccatt aaaaccttct tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt
    20581 aacgatgatt tccgctctcc tcacgtttct catcaaattg gagacgaaaa cggaaaaatc
    20641 agtgttccaa atcaaacgat tctttcgtta tttactgttt cagccctttg aatgttgtct
    20701 agaaaaatta attcccacat gagaaaaaag aaatttggct cgttactttt ggttttcagt
    20761 tgaataacag ataaaaaatt ttctggaaaa agtcatcctc tgcgtcagca cagttttttt
    20821 tgacttattt tcagaggaaa tagttttcag aatattctaa aatgttttat gcaacactag
    20881 tgatattttt tagaattaga tttctaatgt gcattttttg agtattaagg agcaagtata
    20941 caaagattta taaagctgat taaatttgaa taaaaatatg caggaacgct actattttta
    21001 gtagtgttct tttttatatt tgaataaaaa atgtggtggt taaatggtgg ttataacaaa
    21061 aaatgaatta atgaaattag gttatgggaa gtatcagtct gagagaatca tacgacaggc
    21121 aaaagcaatt atggcttcaa aaagctatac ctattacgaa aatagaagat taggactagt
    21181 acctgttgaa tctgtagaag aagttttagg tatgaagata gaggtagagg aggattttca
    21241 gaatcatggt taattttaaa aaagataaaa atgggaaata tcattttact gctagtttgg
    21301 gattcgacaa acgaacaggg aaaagagtac aaaaaaacgt agaggggtta aaacagttaa
    21361 agaagaaaaa aagcgtatac cgaacttgtt tctaattatg gaaaagaagc agaaacttct
    21421 caaagtacta tgttgtttga ggagtttttt tataaattat tcctacctta ttatcaaggg
    21481 aaagtagaag aaagaacata taaaaatcgg ttatctacaa tcaaaaaaac ttttcttatt
    21541 tctttaaaat ggagttaaga gaaatacgtc caattgacat tcaaaagtgg caaaataatt
    21601 tattgagcaa atataaaaat tcttatgtaa gaaacatgta tgggattttt cagatgtcat
    21661 tagatcgagc agttgttttg ggactacttg aaaaaaatcc tgcaaaagtt attggaaatg
    21721 tgaagaaaag gaaaactaca gttgattttt ggacaaaaga agaatttaca aaagttattt
    21781 caacttttta tattgatgat ttgtaccaac attttggttt tatttgtatg tggttacttt
    21841 ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac ttggaaagat attgattttg
    21901 aaaataaaaa cttgcgtgtg aataaaacac tctattataa gagagccaaa acctatgagt
    21961 ttgtagaacc cgaaacggct tctagtattc gtacaatcga actagacgac aatacaatta
    22021 cctttttaaa agcttggaag gagcgtcaag tttcaatagg aggcatgtcg tttgtacttt
    22081 cttataatgg aacacctact cagaaatata caatccgtca tattatgaga agacatgcaa
    22141 cgatcgttgg tgttcatata atccgcatac acggattaag gcatagtcat gcttctttgc
    22201 ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag gttaaaacat gctgatgtac
    22261 aaacgacttt aggaacttat ggtcatttgt atctaaattc attgagagag ataacaaatg
    22321 atcccacaga ttatatccaa actaattttt ctaaaatcaa accgacacct atttacgata
    22381 ataaaaaata aatgataaac aaaagtagga tcacaccaaa agaaacacac tagaaaaact
    22441 ataatacagg cttttctagc gtgttgtttc ttatttccgc ctatttatac attatactca
    22501 taaagcattt aatgcatcga tcgtagccgc attgtctata tgggtatata aagttgttgt
    22561 attggtactt gaatgtccca attgttgggc agttaatacc aatgaatttg tctgttggta
    22621 gagtctagta gctaatgtat gacgcagttt gtgagggctg acttgaattc taaagccttt
    22681 tgaatatttt gctaccatcc gttcgattgc aataccggat atacgcttgg cttctccttg
    22741 gtaaattgct agaaataaag ctttttcgtt ttctgttact ttatatcgtt ctttccgaat
    22801 agctagataa ttagctaaat attccattgc gaatgctgct atattgacag catctctttt
    22861 atttcctttt ctagttacta ccacattatt cttatctaaa gataggtcat ctaaattaat
    22921 atttataacc tcggagagtc tcaatccaga tcctaaaatc agtgcattga tcgccaggtc
    22981 tctttctaca tctcgttgat aatagatcaa agctcgttta ctcaaagtat ttttatattc
    23041 attggcaata taatctagat aattttgtgt atccttatct agaaataatt ttttctcaat
    23101 ggcattggca cgtgcagcta acgtagtttt atctgtgatg gttgctactt gatccatagg
    23161 attattgtct aagtatggtt ttcctagagg attattggaa cttttcgaaa gatatttaaa
    23221 tagaaccttt aatgccgtga tatttctatt gatagtagaa gtagatagac ctaagttatt
    23281 attatctttg agcttagttt ttgttgtctc tttttttaca ttttcttttg tgcgacgttt
    23341 caaatgagat ttgtacaagt ttaggtcata ggcagttaac tgtcttatcg aggtgataga
    23401 agtatcagga acgatagact cactcagaag ccattcataa aagagcttat aattttttag
    23461 atattctaaa gtggttcgag gtgattgatc tttatcctca cgatcttgaa caaaatcttt
    23521 tatataatcg ggcatatgaa gtatgattgt atctattttt tgatatattt ctgtattttt
    23581 catgtcattc atcctttcaa taagtagggg agaagagtat ctctataatt ggactatacc
    23641 aattatagaa tgttttgtca attggtatgc tctaaaattt attaatttgt ttcaaaaaaa
    23701 gcatataaac aggtatacgt ttaataattt aaaataaatg gtatacataa atagggaagg
    23761 aaatagaagg gagcaatcta aattttttga attttcttaa ttaattatat agaaagaaca
    23821 atctttctat agagttagac atgagaggga ctatgcttga tttaaaatca acaagcgtat
    23881 atactgtata tacaaagaaa aaggagggag aagactatgg caattaaaga aagaaaattt
    23941 agggaagtgg ggaactcagt agtattaact atttcaaagg agtttcttaa agaaaatggg
    24001 ttaaatcccg gagatacggt ttttttagat gaagaaaaac ttcgagcggc aattacaaaa
    24061 aaacaagcct tatctgatga aattgattta gcaatatctc aagcattaac tgaatatgat
    24121 actactttta gagagcttgt cgataaatga gtattcatta cctagaagcc aaagatatta
    24181 taaaaatgaa tgtaatccaa ataaaaaaat actctcctaa tgaacccctt ggtgtaaagg
    24241 atgcgaatgc tttagaaatg tgtgtagggc aacttcgagc ctcagctttt ggtgaagagg
    24301 tttatccttc gatttatgaa aaagcagcta tcctacttat acaattgata aagaaacatc
    24361 cttttcacaa tgcaaataaa cgtactgctt tcttagctac ttttgtcttt ttgaaaatca
    24421 atggacagct tttagctatt gatcaaaaag atgctgtaaa tttagcggta tatattgcta
    24481 cgtatgataa ggatttcgat caactaaaat atgaagtgat caacactata aagacacata
    24541 cgcagcccat ttaaggagtt actgtatgtg tctttatagg gttattagct gctatagcga
    24601 aataattgaa gtaaattaca aatagctata attcaaatcc aactatatag aaaacgtaaa
    24661 ttttctatat agttggatta agataacgac tatttatttt atcatatttt tactataccc
    24721 tatactttta cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg
    24781 taagttagat taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact
    24841 tataaattga tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt
    24901 tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac
    24961 ttatgaaatg aaagaccttt tacatcttgc agaaatcgtt actgggatta aactaagcaa
    25021 taaaaactaa tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata
    25081 aatgacgatt tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc
    25141 agtatcagta ataggatagg cgataggcca catacccttt gatggttaga ataaactgct
    25201 caaaatctgt atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa
    25261 ccataagcgt tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata
    25321 taattaccgg acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa
    25381 ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct
    25441 agtctcctaa gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt
    25501 catggtttat ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca
    25561 ttctagggga gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg
    25621 tagtcgtatg ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt
    25681 aaacgggaat ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa
    25741 atggagcaac tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg
    25801 cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa
    25861 tcatcatgtt aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta
    25921 gatgtgcata ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt
    25981 gatcagaagg cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta
    26041 aattcgtcca accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa
    26101 ctcttttatc cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag
    26161 tcctatttat ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc
    26221 cataaattcc taattgtctc ctttaattca tctataattt catccacgat attttaatcc
    26281 gttctacaat gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa
    26341 aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga
    26401 atatatttct tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca
    26461 aaatcatgtt aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg
    26521 ttaagtttag ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt
    26581 aaaactcaaa atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg
    26641 taaaaaatat tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa
    26701 acagctacaa aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc
    26761 atattcacgc ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt
    26821 ttaggaaatt gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc
    26881 accagatatt cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca
    26941 ccgcgaattt actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat
    27001 taagggatca agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta
    27061 aacaattata gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac
    27121 tttcttattc tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt
    27181 acgagtacta ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc
    27241 cccacttagc ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag
    27301 aagaaaacat accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag
    27361 aattcaagca acaaatcgtc aatctttact tagctggaaa gccacgtgta gaaatcattc
    27421 gagaatatga actaac
//
LOCUS       DAYYCQ010000034        26885 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000034 DAYYCQ010000000
VERSION     DAYYCQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.034"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1056
                     /locus_tag="ACHAYF_001416"
     CDS             52..1056
                     /locus_tag="ACHAYF_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578858.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1057..2001
                     /locus_tag="ACHAYF_001417"
     CDS             1057..2001
                     /locus_tag="ACHAYF_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578859.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2001..2963
                     /gene="opp4B"
                     /locus_tag="ACHAYF_001418"
     CDS             2001..2963
                     /gene="opp4B"
                     /locus_tag="ACHAYF_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8578860.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            2990..3904
                     /locus_tag="ACHAYF_001419"
     CDS             2990..3904
                     /locus_tag="ACHAYF_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8578861.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3930..5711
                     /locus_tag="ACHAYF_001420"
     CDS             3930..5711
                     /locus_tag="ACHAYF_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8578862.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6059..6745
                     /gene="rnc"
                     /locus_tag="ACHAYF_001421"
     CDS             6059..6745
                     /gene="rnc"
                     /locus_tag="ACHAYF_001421"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8578863.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6765..10346
                     /gene="smc"
                     /locus_tag="ACHAYF_001422"
     CDS             6765..10346
                     /gene="smc"
                     /locus_tag="ACHAYF_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8578864.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10355..11164
                     /locus_tag="ACHAYF_001423"
     CDS             10355..11164
                     /locus_tag="ACHAYF_001423"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8578865.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11175..12173
                     /gene="ftsY"
                     /locus_tag="ACHAYF_001424"
     CDS             11175..12173
                     /gene="ftsY"
                     /locus_tag="ACHAYF_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8578866.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12360..14627
                     /gene="gshAB"
                     /locus_tag="ACHAYF_001425"
     CDS             12360..14627
                     /gene="gshAB"
                     /locus_tag="ACHAYF_001425"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8578867.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14716..15168)
                     /locus_tag="ACHAYF_001426"
     CDS             complement(14716..15168)
                     /locus_tag="ACHAYF_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8578868.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15318..16268
                     /locus_tag="ACHAYF_001427"
     CDS             15318..16268
                     /locus_tag="ACHAYF_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8578869.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16261..17220
                     /locus_tag="ACHAYF_001428"
     CDS             16261..17220
                     /locus_tag="ACHAYF_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8578870.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17217..17972
                     /locus_tag="ACHAYF_001429"
     CDS             17217..17972
                     /locus_tag="ACHAYF_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578871.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            17995..18948
                     /locus_tag="ACHAYF_001430"
     CDS             17995..18948
                     /locus_tag="ACHAYF_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8578872.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19592..19888)
                     /locus_tag="ACHAYF_001431"
     CDS             complement(19592..19888)
                     /locus_tag="ACHAYF_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8578873.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20243..20599
                     /locus_tag="ACHAYF_001432"
     CDS             20243..20599
                     /locus_tag="ACHAYF_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8578874.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20609..21508
                     /locus_tag="ACHAYF_001433"
     CDS             20609..21508
                     /locus_tag="ACHAYF_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8578875.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22028..22756
                     /locus_tag="ACHAYF_001434"
     CDS             22028..22756
                     /locus_tag="ACHAYF_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8578876.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22901..24928
                     /locus_tag="ACHAYF_001435"
     CDS             22901..24928
                     /locus_tag="ACHAYF_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8578877.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25036..26133
                     /locus_tag="ACHAYF_001436"
     CDS             25036..26133
                     /locus_tag="ACHAYF_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8578878.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26440..>26885
                     /locus_tag="ACHAYF_001437"
     CDS             26440..>26885
                     /locus_tag="ACHAYF_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578879.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         9013 a         4658 c         5680 g         7534 t
ORIGIN      
        1 ccttttttat taagaaaaac ttccatttag gaagcagaga ggggtattcg attgtccgat
       61 aaccaattat tagaagttca gaacctacat acaggattcc gactcaaaga tgactattac
      121 gatgcagtag atgacgtttc atttacttta gacaaaaacg agattttagc aatcgttggg
      181 gaatctgggt gcgggaaaag taccttggct actacgatta tgggattact tgatcctaat
      241 aacacaaaaa ttacaggtga gattttatat aacgatctga atttaatcga cctgaacgaa
      301 acgctctata ataaaatccg cggaaatgat atcggaatga ttttccaaga tccgttgtct
      361 gcattaaatc ctctcatgag aatcgaagac cagatcaaag aaggactttc ctatcacaca
      421 aagatgaatg cagaacagcg tcaagctcgt gcactagaat tgctagaaca agtagggatt
      481 cctaatcctg cgagagttgg acgccaatat ccccatgaac tatcaggcgg tatgagacaa
      541 cgtgtcatca tcgcaatcgc aatcgcctgc aaacctccaa tcgtcattgc agatgaaccg
      601 acgacagccc tagatgtaac catccaagca cagattcttg atttactgaa agaccttcag
      661 gaagaaacaa aaacagggat catcttgatc actcatgact taggagtcgt agcagaaatg
      721 gctgatcgag tggcagtgat gtatggcgga cagttcgttg aagtagcagg tgtaaaagaa
      781 ctgtttgatc atcctaagca tccttataca cgctctttac tgaactctat accacaagaa
      841 ggaaatcatg atgcagaact ccatgtgatc gaaggtgtcg ttccttctct gaaaaacatg
      901 cctcgtaaag gttgtcggtt tgcaccacgt attccatgga taccagcaag cgatcatgaa
      961 gaagagccta tccttcatga agtcgcaccg aaccacttgg tacgttgctc ctgctataag
     1021 catttccact ttagagacga aaaaggagag gtgtagatga cagaattaat cacaatcaaa
     1081 gacctgaaag ttcattaccc tattcgcagt ggattcttca atcgagtaac ggatcatgta
     1141 tatgctgtag atggtgtgga ttttattatc gaaaaaggaa agacatatgg tttagtggga
     1201 gagtctggct ctggaaaatc tactacggga aaagccgttg tgggattaga aaaggtaaca
     1261 gccggacaaa tcatgtatga aggaaaagat gtaacgaagc gaagcaaccg caaaaaaatg
     1321 ggttataaca aggatgtgca gatgatcttc caagattcaa tgtccagttt gaatcctaaa
     1381 aagcgagtgt tggatatcat tgctgaaccc atccgaaatt ttgaacggtt aagcgatcaa
     1441 gaagaaaaga aaaaggtaaa aggactgcta gatatcgttg ggatgccgga agatgcgcta
     1501 tataaatatc ctcatgaatt ttcaggcgga caacgtcaac gtctaggagt agcccgtgct
     1561 gttgctacca atccaaaact gatcgtagcc gatgaacctg tatctgctct ggatctatct
     1621 gttcaagcgc aagtattgaa tttcatgaaa cgaatccagc aagaatacgg cctcagctat
     1681 ttattcattt cacatgattt aggtgtagta aaacacatgt gcgacaatat cgcaattatg
     1741 tacaaaggtc gatttgtaga aatcggtaca agagaagaca tttataatga cccgcgacat
     1801 atctatacga aacgtctgct ttcagctatt ccgcaaatcg atgtagaaaa tagggaaagc
     1861 cacaaagaaa atcgacgaag agtagaacaa gagtatatcc aaaaccaaaa agaatactat
     1921 gatgcgactg gtcgagtcta cgatttgcgt acgatcacaa ctacacacaa agtggcgttg
     1981 aaagatggag gtgcaagcta atgtggaaaa cgattctgcg acgtgtttta ttgatgatcc
     2041 cgcaagttat tattttaagt gttctgatct tcttattagc ccaagcgatg ccgggggatc
     2101 catttaccgg tttgatcaat ccaaatcagg atccggcagt gatcgaacag atgcgacaag
     2161 cagctggctt gaatgaccca tggtatgtcc agtacttccg ttggatcgga aatgctttgc
     2221 aaggcgattt tggtcaaagt ttcttataca aacgctctgt tgctgacttg atcggcgaac
     2281 ggatcatcaa cactttatat ctatcgatct tgacagttgt ccttacttat ttgattgctg
     2341 tgcctctggg gatgcttgct ggccggtatc aaaattcaat cttggataag attgtcgtaa
     2401 tctacaactt ctttagtttt gctgttcctt tatttatctt cggcttgatc atgctatttg
     2461 tttttggtta tcgtttaggc tggttcccaa caagtggatc acaaacacta ggtttgcaag
     2521 gtggatttat cactcaatgg atagaccgtt tgaaatatat tctgttacca tcaattaccc
     2581 aagcattttt ggcgactgcc gtcacaatcc aatatctgcg aagtgaagta atcgattcga
     2641 aatcgctcga ttttgtcaga acagcccgtt ccaaaggggt accgacgaat aaagtattca
     2701 gtcgacatat tttccgaaat gcagcgttgc cgatggcttc tcaaatgggg tatgaaatca
     2761 cttcgttgat tgctggttct gtagttatcg aaaagatttt tggttatccg ggagtcggaa
     2821 aactatttat tgattccatc ggccaacggg actataccgt gatcacatcc ttagtattga
     2881 ttttaggaat cgcgacgcta gtggggacat tactttcaga tatcatcatg agtatcgttg
     2941 acccgcgtat tcggattcaa taaaaccaat cagaaaggag aatggatcga tgagtgaaaa
     3001 aaacaataca gcagttcaag aaagtattcc tcctatgggg atacgaatga ttgctcgaga
     3061 atttaaaaaa gataaaattg ctattttttc attaggtttg ctagtggttt tattattagt
     3121 gatctttatc ggtgcgatgc tgaccgatca agacaaagtg atgtatgtta gtatcttcga
     3181 caaatacgct gcaccaggaa cagtttcgac acaaggacaa aagttcattc tgggagcaga
     3241 tgaaggcgga cgtgatgtat taggacaatt gatcataggt gctagaaact ctgttacgat
     3301 cggatttgcg atcacgatcc ttacttcgat catcggggtt ggattaggga tcatctctgg
     3361 ctactacgga gggatcattg acaacatctt gatgcggatc gttgatttta tcatgattct
     3421 accgatcatg ctgatcatta tcgtcttcgt atcgattatt tccagataca atgtctggtc
     3481 ctttatattc atcatgagtg cattttattg ggtagctaaa gcccgattat tccgaagcaa
     3541 gacattatct gaagcaagaa gagattatgt cagtgcctca aaaacaatgg gaaccaatga
     3601 ttttaaaatc atgttccgcg aaatcatgcc gaatctaagt tcattaatca ttactaattt
     3661 gacgatgaac tttgcggcaa acatcggtat cgagacaact ttgactttct taggtttcgg
     3721 cttgccggca aatgtaccta gtttaggtac attgatcggt tatgcaagta acggagaagt
     3781 cttagtgaat cggcaatgga tctggttacc cgcatcaatc cttattttag ttttgatgtt
     3841 gagtatcaat tatgttggac aagcattcaa gcgttctgca gatgcgcgcc aacggttagg
     3901 ctagtataga agcgaagggg gaaataggaa tgaagaaaac gatttttgga tttgcagcgc
     3961 tgatgtctgc tgtcgttctt gcagcatgcg ggggcggagg tacagatacc tctggaaaca
     4021 actctggagg caacggcaat aacaaagagg aattaacttt tccaattgca acaaaaaata
     4081 atgatgatgc aatcgaagac ggcgaattag aagctgcggt cgcaacggac agtcagttta
     4141 aaggattgtt ccagtgggag ttttaccaag attcatatga tgcagctttt atggctcctt
     4201 ctcatgaacc gctatttgca aacgatgacg atttcagaat cacagacgac ggagctgcaa
     4261 cgcttgatct agatgaagat acgaaaaaag cgacaatcaa agtaaaagac aacgtcaaat
     4321 ggtcagatgg tgaagatgta acggcggacg atgtgatttt cccttacgaa atcattggga
     4381 ataaagatta cactggtatc cgttatgacg acactttccg taacatcgta ggtatggaag
     4441 aatatcatga tggaagtgcc gacacaatct ccggaatcaa aaaaatagac gacaaaacag
     4501 ttgaaatcga atataaagaa gtaaacccaa gcatgcttca atcgggtggt ggtatctggt
     4561 catatgcagc accaaaacat acgttagaag gtgtagcaat caaagatatg gaatcaagcg
     4621 atcaagtacg taaaaatcca gtgacttttg gcccttatta catgagtaat atcgttgcag
     4681 gagaatcagt agaatacttg ccaaacgagc actactggaa tggtacgcct aaattgaaaa
     4741 agatcacgat gaaatctgta ccgatggcaa gtgcaactga agcattgaac tctaaattat
     4801 atgatatgat tttccaaatg ccaacagata cttacgatac ctataaagaa attgctggct
     4861 atacaaactt aggccgtgaa caaacttctt atacttatct aggattcaag ttaggtaaat
     4921 ggaatgcaga tacaaagagt gtggaataca atccagattc aaaaatggct gataaatcat
     4981 tacgtcaagc aatgggctat gcattagata ataatgcagt aggtgaacgt ttctatgcag
     5041 gtttgagaag caatgcgact tcattgattc ctccagtatt ccagtcatac catgatgatg
     5101 agatgaaagg attcacacaa gatctagaca aagcgaaaaa actattagat gatgctggct
     5161 acaaagatgt ggatgacgat ggtttaagag aagacaaaga tggaaataaa ctaacaatca
     5221 actttgcttc gatgtccggt ggggaaacag cgcaaccatt ggcagattac tatatccaac
     5281 aatggaaaga aattggtttg gatgtccaat acacaacagg tcgtctgatc gaattcaatt
     5341 ctttctatga taaattagaa aacgatgatc ctgaaattga tgtttaccaa gctgcttgga
     5401 atacaggaac tgatccaaat ccaagtggac tatggggacc aaatgctgcg ttcaactaca
     5461 ctcgttatga atcagaagaa aatacaaaat tgatcaacga tatcagttca agcaaagcat
     5521 ttgatgaaga gtaccaaaaa gaagcatatc aaaaatggca acaatatgca tttgaagaag
     5581 catttgcgat tccaacgttg ttccgtaatg aagtattgcc agtcaacgac cgtgtcaaag
     5641 actgggattg gtcttatgat gtaccaaacc catgggcaaa tgtttctgtt acagcagatt
     5701 cacgttcata atcactaaaa atgaccgaaa aagggcgatg tcctttttcg gttttttgat
     5761 atttttctgt ttatttctct aatattcagg tcaaggcgct gatttcatga cttctccaat
     5821 aaacggtgtt caaagtccag tttctcggta ttaagtcaaa tttatgcaaa aatatggaaa
     5881 gccattttca caaaattttt tcttcatact cgaaactaaa cgaatttttc acaacttctc
     5941 gtcaatcact aggattatta aagctttact taccttttga ccgaaccatt tcaaatttga
     6001 caaaaaacca gtataataag gaagtatcga aaaaatatcc ataaaaggaa tgtggaaaat
     6061 ggacaaccag ctgacaaaag aactaaaaga aaactataac atcgtttttc acgatctaaa
     6121 tttgcttgaa caagctttta cccattcatc ctatgtgaat gagcatcgca atctgcaatt
     6181 atcggataat gagcgacttg aatttttagg agatgccgtt ttagaactga tggtttctca
     6241 gtatttattc cgtttatatc ctcatttgcc agaaggaaaa ttgacaaaaa cacgagctgc
     6301 gatcgttcgc gaagacagct tgtcgaaatt tgccaaagaa tgtcactttg atcaatatgt
     6361 tttgttgggc aagggagaag agaattcagg aggacgtacg cgaccagcat tgctttgcga
     6421 cctattcgaa gcttttttag gcgcgttata tcttgaccag ggattcgaca cgacccatgc
     6481 attcatcgaa aaagtgattt ttcctaaagt caaagcaggt gctttttcac atgagatgga
     6541 tcataaaaca aaactacaag aagtcttgca aaaatcaggt gatgtatcaa tcgaataccg
     6601 attgatcaat gaagaaggtc cagctcatga acgagtattt tggatcgaag tttatgtaga
     6661 tgatcaattg attggcactg gacaagggaa atcgaaaaaa ttagcagaac aagctgcggc
     6721 ggaaaatgca ttggcagcac tgtaaaagta agtgagggaa agctttgtat ttgaaacgaa
     6781 ttgaaatagc cggttttaaa tcatttgcag acaagacagt cattgatttt gaaaatagcg
     6841 tcacagcagt ggttggacca aatggcagcg gaaaaagcaa tattacagag gctatccgct
     6901 gggtattagg agaacaatct gctaaaagtc tgcgaggcgg aaaaatgccg gatatcatct
     6961 ttgccggttc cgatacccgt aagcagctga atattgcaga agtcacagtg attttagaca
     7021 atagtgatca ttatctgcca ttggagtaca atgagatcag tgtaacccgc cgttatcgac
     7081 ggactgggga aagcgagttt ttcatcaata aacaaagctg cagattaaaa gatatccaag
     7141 aattgttctt agattctgga ttaggaaaag aatcgttctc aatcatttct caaggaaaag
     7201 tagaagcgat tttcagtagt aaaccggaag accgtcgtgg gatctttgaa gaagcagctg
     7261 gtgtcttgaa atacaagcag cggaaaaaga aagcagaaca gaagcttttt gaaacagaag
     7321 acaacctcag ccgcgtccaa gacatcatcc acgaattgga agaacagtta actcctttgg
     7381 ccgcccagag tgaggcagca aaggagtttt tacggctaaa agaaacactg actcaaaccg
     7441 atgtctcttt gatggttgct gaaatcaaga cagcaaaaaa agattgggac aacaaacaag
     7501 cacagcttgc gaaattcaat ctagaattag gcaaactatc tgagtcgatc caagaacaag
     7561 aatccatttt ggcgaagcag cgtaaagaaa atgcgcaagc cgatcgtctg atcgaaaaaa
     7621 atcagcaagt attattagat ctttcggaaa aactgaagca gacagaagga caaaaagatg
     7681 ttttgcagga gcggacaaag catacacaaa aaagctcgca agaatatcaa acttcattag
     7741 cagaagcaca aaaaaaggtt aaacattttg aaaagcttca agaaagcttg atgaaagcag
     7801 cagcagaaaa agaaacagag atccaaaaag ctgaagcaaa tctcataaaa acacagcaag
     7861 aattagaaaa ataccaaaaa tcaacgaaag aattattggc agaattaaga gatcaatatg
     7921 ttgatttgat gcaagaacaa gcagctgtag gaaacgaact gaagtatcta gaacgtcaat
     7981 atatacaaga aacagcaaaa agcaagcaga cgttagccaa gcaatcggaa gtagaagcat
     8041 cagtagaccg attgatctta caaaaagaag aattgacaca aaagcaggcg cagctaaaat
     8101 catctttaac tgaaacaaaa gagaagttag aaatgatcca acaaaacggg aaaaagttcc
     8161 aagaagcatt agcaaaagaa cagcctaaaa tgtatcaatt aatgaatcaa gtccagcagc
     8221 tacgtgctcg taagaaaagc cttcaagaaa tccaagaaaa ctattttggt ttttaccaag
     8281 gggttcgatt ggtcttgcag cataaacagc agctttcggg tattgtcgga gcagtggctg
     8341 aactgatcga tgttcctgca gatttcacgt tagctatcga agcagcttta ggtggggcag
     8401 cccagcatgt catcgttgaa aatgaaaaag atgctcggca agccatcacg tatttgaaac
     8461 agcaaagagg aggaagagca acattccttc cactgacgac aatcaaacca agacaattac
     8521 cggctcatat attgacacaa gcagcagctg tagaagggtt tatagggatt gccagtgaac
     8581 aagtcagtta tcccgatcaa atccaaactg tggttcaaaa cctgctagga acgatccttt
     8641 tagccaaaga cttaactagt gccaatgcaa tcgcgcaaac gatccgctat caatatcgag
     8701 tggtttcctt agaaggtgat gtgatgaatg ccggaggatc catgacaggt ggagcgaata
     8761 aacgaggaaa ccaaggcagt ttgtttgtac aaaatcaaga attgaaacag ttgactagtg
     8821 aatttgaaga agcagataaa caactccaag cacaagaaaa aaaagttcag gagctgcaac
     8881 aagaaacagc tcgtttagcc gaagaacaag aagttttgcg tacacgaggc gaacaattac
     8941 gttttgaaga acaagaagcg acaaatcagc tacaaaacat cataaatgaa cttgaacgtt
     9001 ttgaaaaaga aaagcaaatc tcaacttttg agacacgtga actgcagcag tttatcgaag
     9061 attacgaaaa gcaaacgaac gaattgaaag acaaacaaac agatcttgaa tcgcaacgac
     9121 aaaaaatcga cgaagaaatc aaatcgttaa gtcaagaaag cgaccagatg gaagccagaa
     9181 gagcacaagt acaaagtcaa aaagcacaag aacaagcaga tcttgctgtc ttgaaagagc
     9241 agtttaatca tctgcagatc cagctccgcg gtgcccgagt ccaaaaagca gaggcaaccg
     9301 aacgtcaaga agcgatagaa aagcagttgg cgacattgac agcagacttt tctgatcatg
     9361 aagtcacaga agaaagtttg gaaaaacaaa ttaacgaatt atctgctcag cgagaaacat
     9421 taaaagccga gttggcaaaa gccaaagagc agcgagatag aaggcaaaag gagatcgata
     9481 agcttgaagc agtcttggca gagcgtaatc gtgagcaaaa agcacgactg tcggaacaat
     9541 ccaaattaga agtccaaaaa gaccgagctg aaatgatttt agataatcat ttgacctatc
     9601 tgcagtcaga atatcagctg acatttgaga aagcttccca agactatcaa gaaacaaccg
     9661 atatcgaaga cagtagaaca aaggtttcat ccttaaaaga acagattgaa aaactgggac
     9721 cagtaaactt aaattcaatc gaacaatacg aacaagtcag tgaaagacat acattcttag
     9781 caacacagcg agatgatctg ttagcagcga aaaaccaatt gtttgaaacg atggatgaga
     9841 tggatgatga agtgcggaca agattcaaag aagtatttga agctatccgt caagaattca
     9901 aaattgtctt tccaaatatg tttggcggcg gtaaagctga attagtactg acggaaccaa
     9961 ctgatttgct gaaaacaggt attgagattg aagtacaacc tcctggcaaa aaactgcaaa
    10021 gtctaagttt gctttctgga ggagaacgag cacttacagc catcgcgttg ctcttttcca
    10081 ttatccgtgt ctgtcctgtt ccgttttgta tcttagatga ggtagaagca gcgttagatg
    10141 aagccaacgt gaaacgtttt ggtcgttact tgagcgattt ccaagatgac acacaattta
    10201 tcgtagtcac acaccgcaaa ggaacgatgg tcgcagcaga tgtactgtac ggagtgacga
    10261 tgcaggaatc cggtgtatca aaaatcgttt ctgtacggat ggaagatatc aatgaagagg
    10321 cgaaaatcaa cgaaacgaaa gtgtaggaac gaaaatgatc aaattagtag caatcgattt
    10381 ggacggtaca ttattaaatg aacaaaagct gatttcagat gagaataaac aagcattagc
    10441 acaagcaaaa cagcaagggg tcaagatcgt tctatgtaca ggacgcccct tagctgcgat
    10501 ggcgcattat ctgcaagaat taggactggt agatgaaggt gactacagca tcactttcaa
    10561 cggcggtttg gtacaaaaaa atgatactgg tgaaatcatg gaaaagaaag tgatggaagt
    10621 atcagatatc caacgcttat acaaactggc tcagcaattg gatctgccac tagatgtttt
    10681 gtctgacaat gtggttttac agctgcctag tgccccaagg aagcaatcct tgtacaatgt
    10741 gttgaataag ctgctgcaat tccaaccagc ggatataagc gatattacag atgaatttgt
    10801 gcttaataag gcagtaatcg cttatgacca agaagaatta gatcctaaaa tcaaagaaat
    10861 accagcagaa ttccatgaaa tgtatgagat catcaaaaca agaagtatgc ttttggaatt
    10921 tatgccaaaa ggtgttacaa aagcttatgg tatttcttta ttggcaaaag atcttggttt
    10981 gaaacaagaa gaaatcatgg cgatcggtga tgaggaaaat gatttaccga tgatccaata
    11041 tgctggacta ggcgtagcga tggagaatgc tgtgcctttc gtcaaagaag cagcagattt
    11101 cgtgacagcc agcaatgaag aaaatggcgt agccaaagtg atccaaaaat ttgtattaga
    11161 gtagggggag tcaaatggga ttttttgata aaatcaagcg tgcttttact ggcgaagaga
    11221 aaacagaaga agttgaagta caggagaagt acgacaaggg tttagaaaag acgcgtaaaa
    11281 catttggtga acggatgaat gagttgtttg ccaattttcg aacagtcgat gaagactttt
    11341 ttgaagaatt agaagaaaca cttatcggtg cagatgtagg ttttgaaaca gcgatacgaa
    11401 tcaccgaagc attacgtcaa gaagtgaaac tcagaaatgc gaaaaaatcg gcagaagtcc
    11461 aaaatgcgat catcgagaaa cttgtggatc tctatgaagc ggaaggaatc aatgaagtaa
    11521 atgaactaaa tatccaagaa gacggactaa cagttatttt gtttgtcggt gttaatggtg
    11581 tagggaaaac aacaagtatt ggtaaacttg cccatcagtt caaaaacgaa ggtaaaaaag
    11641 tattgatggc agcagcagat accttccgcg ctggagcaat cgaccagtta gttgtctggg
    11701 gcgaacgcgc tggtgttgaa gttgttcgtg gcaatgcagg aggcgatcca gcagctgtcg
    11761 tattcgatgc tttggaaaga gcaaagacag agcaggcaga tgttttacta gttgatactg
    11821 caggccgact gcaaaataaa gtgaacttga tgaacgaatt agaaaaaata aaacgagtaa
    11881 tccagcggga attaccagaa gcaccccaag aaacacttct tgtattagat gcaaccactg
    11941 gtcaaaacgc gatggttcaa gcaaaacaat tcaaagaaac aacagatgtg accggtcttg
    12001 ttttgacaaa attagatggt acagcaaagg gcggtatcgt acttgctatc cgtaatgaat
    12061 tgcatttacc agtgaaattg gtcggtcttg gtgaaggaat cgatgacttg gagccgtttg
    12121 atccaaatga ttttgtcgtt ggattgttca aaggattact aaaagaagaa taacccatgt
    12181 ctaaaaagca ggagctgtga caaaagttgc agctccttgt ttgaatgatt gtttttaaca
    12241 tagctgtgtt tttgatgcct tcatttttat acttatttgt aagcggttct caaacaattt
    12301 cgttcttttt cttagacttt ttgattataa ttagattcat atgaattgga ggtcacttaa
    12361 tgatgaattt taagcaatta ttattgcatg tcaatgcgcg cccttttatc gaccaagctc
    12421 gatttggtat agaacgggaa ggacagcggg ttgatcttgc aggaaatcta gcaaaaaccg
    12481 atcatccagc aatctttggc gatcgatcct atcaccccta tatccaaaca gattttagcg
    12541 aaacacaaac agagatgatc acccctgtta ccgattctat tcccgaatta tttcagtatc
    12601 tcgctgctgt ttatgatgtg actgctcgtt ctataccgaa agaagagatg atctggccat
    12661 taagtatgcc acctgcctta ccggaaaaag acgaagagat cattattgca aaattaaaaa
    12721 atttcgaaga tgtcttgtat cgacgttatt tagcaaaaaa atacgggaag cgaaaacaga
    12781 tggtaagcgg tattcatttc aattttgaat ttggcgatga gttgctaaga acattgttca
    12841 gccatcagga agaatttcaa gatttttccg aattcaaaac agaactttat ttgaaaacag
    12901 cgagaaactt tatgcgctat cgctggatga tcacttattt attcggtgct tcaccaatga
    12961 gtgagaagaa ctacttttta gatgaatcac atccgcaaga acctgttcgc agtattcgca
    13021 acagtgcgct gggctatacg aatcatccaa atgtgaaagt ttcctatgct tctatgaaac
    13081 agtatttagc agatatcgag cgaatgatcg aagaagggaa actttcggag gagaaagaat
    13141 tttatacacc gcttcgtttc cgaggcggaa agaaagtcgc agatttagca acaacaggtg
    13201 ttcgttatat tgaattgcgg aatatcgatt tgaatcctta tgcaagattg ggtatcaatc
    13261 cagagcaagt tcggttctta caattattcc tgatgtacat gttatggaca gaagaaaaag
    13321 aagactgtga ccaatgggta gcagaaggta cgactcgaaa taacaaagta gcactagaac
    13381 aaccatcaga tcaaacagaa tttcatcaag aaggtagaga aattcttgaa ggaatgaagc
    13441 aaatgctggt tgaattggat tggttggatt ctctttattt agtagaggaa gcattgactc
    13501 aaatggatca tcctgaacaa acgttagcag cgaaactcta tcaagaagca cagctatcga
    13561 gtcagcaaga agttgctgtg gcattgggac atcaatatta taaagaaagc catgaacgtc
    13621 cctatcagtt ggccggtttt cgcgaaatgg aactttctac tcagatattt atgttcgatg
    13681 ccatccaaaa aggtgtccaa gtcaaagtat tagatgagtc ggatcaattt ttgcgtctgc
    13741 aattccaaga ccatgtagaa tatgtgaaaa atgcaaacat gacaagtaag gacagctata
    13801 tcgtcccact tatcatggaa aacaaaacag taacgaaaaa agtcttaaaa gaagctgggt
    13861 tccgggtacc aggaggcgca gaattttcat ccatggaaga agcggtaaaa gcttatccga
    13921 gatttgccga gcaggcattt gtgatcaaac ctaaatcaac caattacggg ttaggtatca
    13981 cgattttcaa ggaaggagct agtctggaag attatcaagc aggtttagcc attgcttttc
    14041 gtgaagacag ttcggtttta gtagaagaat ttatgccggg aacggaatat cgtttctttg
    14101 tgatcgacgg agaagtccaa gccatcatgt tgcgagtccc tgctaatgtt attggtgaca
    14161 gtatccgaac agtgaaagaa cttgttgaag aaaaaaatag tgaccctttg cgtggaacga
    14221 atcatcgtgc acctttagaa ttgatccaat taggtgaatt agaacaattg atgttgaaag
    14281 aacaagggtt gacgatcgaa tcagttcccc aagccaatca aattgtttat ctaagggaga
    14341 actcaaatat cagtacaggt ggtgattcga tcgatatgac tgatgaattt tctgaagctt
    14401 acaaaaaaat cgccgtttct gcggtagaag cactaggagc taagattagt ggtatcgatt
    14461 tgattatacc agataaagaa atcgatccta caactgataa aaaagcatat gggatcatcg
    14521 aagcaaattt caatccagcc atgcatatgc atgtttatcc ttttgcagga aaaggcagaa
    14581 gattgacgat gaatgtattg aaattgttat atcctgaagt attttaaaag aaaaagcttt
    14641 tgacagattg tcctatttga tgtcaatctg atcaaaagct ttttttgcat gtaaaaattt
    14701 atgtgttttt tcgttttaaa ataaacgatc ccagaagctt tttttctttt ttggtttttc
    14761 tttttcagct ggcgaagagt tttcttctgg gtattttttt tcgatatcaa tcacaggttc
    14821 atttactgct tcttttgctg ttaagagcaa tccgaattcc tcaggatttg cagtgacgta
    14881 atcgttgact acagtgaaac ggctattgtt tttcgttgct agctggatat attggttttg
    14941 gacagaaagg ggtagagccc cattcaagag tatggaagca tctggatgtt ttttgagttc
    15001 ttttaaaaag ttttctttgt tttttggttg gatcatttca gcaacagtca tactaagata
    15061 gcagcgttcc ctgaatgtcc ctaaatattt atgctgttct tcagggttca ctaaaggtgt
    15121 accataaagt cccttatcca aatggctttt tacatcatct tgtgccataa aaaataccct
    15181 ccttcttctc tttgtctttc ttacgattat agcatattcc caataatacc taaaacatat
    15241 gaaaaagcgg ttgcttttgt tcaacgattc acttaaaata agaatgaaaa tcattctcaa
    15301 ttagaaaggt ggattggatg cgctacggac gactagtagc attattagtt attttaagta
    15361 gtatttcatt gttcatcgga gtatcgaatg tacaattgtc agaactgctc cagttagatg
    15421 ggcaaagctt acaaatcttg ctagtgactc gtttgccacg aacgatttgt ttgatccttg
    15481 tgggagcgac cagtagtatt tgtgggttga tcatgcaaca tttgacccaa aataaatttg
    15541 tttcaccaac gactgcgggc acgatggata gtgcgcgttt aggtatttta gtggctatga
    15601 ttttcctacc tggtgcatcc ttgattgtcc gatccttgac agcattttgc tttgcttttg
    15661 caggaacatt gcttttttta tcccttacgc gattgttcct ccagaaaaat caattgatgc
    15721 ttccgttgat tggagtcatg ttagggaata tcattggttc agttgctact ttttttgctt
    15781 atcaattcca gttggtacaa aatatgactt catggcttca agggaatttt gcgactgtga
    15841 tacgaggaaa ttatgagtta ttgtatgctg ttgttccatt gttgctcttg gtttggctat
    15901 ttgcttaccg atttacgatc attggtctgg gcgaatcgtt tgcagtaaat ctgggaatag
    15961 attaccagaa gatgcagttt ttaggcattg gcttagtatc attggctagt gcagtcatgt
    16021 tgatcatggt aggaagcatt ccttttatcg gagtgatcat tccgaatctt gtctctagaa
    16081 tatatggaga tcaagtgcac aaaacaattg gcgtaactgc tttagcaggt agtttgttct
    16141 tagtattttg cgatcttgtc gctcgcgtgt tgatttttcc ttatgaaata ccagtcagtg
    16201 tgatcgttgg aatcatcggt ggggctatct tcctttatct tctgataagg ggacgacgat
    16261 atgcttaaaa aaaagccatc tatttttatt ggtatgcttg ttcttgtttt atttgttaca
    16321 ggaatgtact tgacgatcca tacatatgga aactggcatt ttgcatgtgc attgaggagt
    16381 aagaaaattt ttgcatttat ccttgtctcg ctagctgtca gcagtgccac aatcagcttt
    16441 cagacaatta cgcataacca gtttttgact ccttcagtat tgggacttga gtcgttatat
    16501 gtccttgtcc aaacggtcct tttcttcttt atcggaggag tgacgatgct tcagcagaca
    16561 ggtacagttt ttttcttatt gaacgttggg ctgatggtcg ggctgagtct attgttattt
    16621 ttctttcttt tgaaaaaaat gagagcgaat ctttttttat tactgatgtc agggatgatt
    16681 ttaggtacgc tttttggtag tgtcagtacc tttttacaag tcatcatgga tccgaatgaa
    16741 tatgatttgc ttcaaggcag attatttgca agctttggca atatccagag tcagtatctt
    16801 ttgcctgctt ctttgatcat tatcggctgc tggtacctgt tatttcgata tcgacatgtg
    16861 ctagacgttt tccatctcgg aaatgaacaa gcagtaaacc tcgggataga cacagatcgt
    16921 tttgaatttt tgattttatg ttgtgtcagt cttttgacag ggacatcaac ggctcttgtg
    16981 ggtccagtta cttttttagg gtttattgtc gcaaatatca gttatcgtgt cttttctact
    17041 tatcgtcatg gtcagctatt tatcggaagc tttctggtag ctatgctgtt tctgacagga
    17101 ggacagctcg cagtagagca gattttcggc tggaatacaa cagtgagtgt cgtcgttgaa
    17161 tttgtcggag gaatttattt tgtcacaaaa ttgattcgcg aaaggaagag tatcctatga
    17221 ttgatttaca aaaggtcaat aaactgtacc atggaaaatc agtggttaaa gatgtacagt
    17281 taacgattcc tgaaaagaag ttgactgctt ttatcggtcc aaatggtgct ggaaagtcaa
    17341 cggtgttgtc gatgatcagt cgtttgatac caaaagatac aggtgaaatc tatttagacc
    17401 ataacgaagt aaaggcttgg aaatccaatg aattagccaa aaaattatcg atcctacgac
    17461 aatcgaatga aataaatgtc caaatcaccg tgaaagaatt agtagaattt ggacgatttc
    17521 cttatacgaa aggaaaattg acgacaaaag acgaggaaat cgttcaggaa tcactggaac
    17581 accttgggtt agaagaaata gcggatcaat cgattgacac tctttcaggc ggtcaactcc
    17641 aacgtgcgta tattgcaatg gtgctagcgc aagacacgga ttatatttta ctggatgaac
    17701 ctttgaacaa tctagatatg aattttgcgg ttcaaatcat gcagatttta aaaaatttag
    17761 tacaagaaat cgggaaaaca attgttattg ttcttcatga tatcaacttt gctgcaagtt
    17821 atgcagacga aattgttgca atgaaagatg gacagttatt tacgcaagga acgacccaag
    17881 aaatgataaa aaaagatgta ttggatgaat tatacgaaat ggacatacgg atttgtgaga
    17941 ttgaaggccg cagattctgt ctttatttct aaaactaaca ttaggagaga agagatgaaa
    18001 aaattagtag taggtatctt gattctagca tcatttggtt tagcagcatg tggaaatagc
    18061 aacaacacca gtcaggcaga cacaaaaaaa agcagcacac aaactgaaac tacattgacg
    18121 ataacagata gtaatggaga ccaaatcgag gtgccaaata atccagaaaa agtcgtcgta
    18181 ttcgacaatg gttctttaga tacgatggat gctcttggtg ttggagataa ggtagttgga
    18241 gcagctacaa gcagccttcc tgagtatctt tcatcctata aaaaagtaga atcagcaggt
    18301 ggaataaaag aaccagactt agaaaaaatt aatcagcttc agccagatct aatcatcatt
    18361 tctggccgac aaagagattt tcaaaaagat ctaagtcaaa ttgctccgac cattttcctt
    18421 tctttagatg cgaaaaatcc atgggaatct ttccagcaaa atgtcactgc tttaggggag
    18481 atctttggta agcaagaaga agctaaaact caattagagg aattatctag tgcaatcgat
    18541 cagacgaaaa agaaagcaga agctactgac aaaaaagcac tagtcacact tgtgaatgaa
    18601 gggcaattat cagcctacgg atctggttct agattcggtt tcatccatga tttatttggc
    18661 tttgaacaag cagatgatca aatcgaagca tccactcacg gtcaaagtgt ttcttatgaa
    18721 tatgtgctgg aaaaaaatcc ggatatctta tttgtagtag acagaacaaa agctattggt
    18781 ggagacgaca gcaaagatga tatttctgcc aatgaactgg tagcacaaac gaatgctggt
    18841 aaaaatcagc agatcatctc ccttgaacca gatgtctggt atttaagcgg gggcggttta
    18901 gaatcaatga aactgatgat cgaagatgtg aatcaagcat ttaaatagca taaataacag
    18961 aggattaaac tctcatacta tgtaacaata atgtttctta cattcttaaa tagcaagagc
    19021 ctatcctcaa agaagttatc aaaaaccatc cgtaaaatcg tattgatgat tttacggatg
    19081 gtttttattt atttgagaaa tcttttttca acgtgaattc attatctaca tcatacgggt
    19141 tgattctaga atggggagtg gcaaaaaata gtgtgatcca ttttttcagc atcaagatcg
    19201 cctcctttga tttgaatcaa aggtacaaag cgaaacttag ataaaactta aatttagata
    19261 tttttatttt gtaccgatct cttctttaaa cgatgttcca tctgctgctt ctcggcaata
    19321 agtccactag actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa
    19381 acaaatcgca gttgtcacaa tctcttctaa acgatgttcc atctgctgct tctcggcaat
    19441 aagcccacta gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa
    19501 agcaaatcgc agttgtcaca atctcttcta aacaatgttc aatcagctga ccttcggtat
    19561 tagctcaaag cactgatttc acaacctcta tttaggcatg tgtctcagca agcggttgat
    19621 agttgatgat ctgcattttt cgataaaaat cggcgggaag cttactccac cgttttagta
    19681 attgatagaa ataactctct tctgctttga ttttatactc gcttaatatc aatccaaaac
    19741 tattcgtttt ttttgaagct aattttttat aaatatccaa tgctttggtt ttatcttcat
    19801 tgacaaagag tgtttttact tcaatataag aaccctcgat attttgtgtt tcttcgataa
    19861 aatagatgtt ttttatatat tccttcatag tgattcctcc aaagaagtct tttatttgat
    19921 aagtttactc taccgaagag ttgttaataa tccgtgaaca aaaagtaatt ctttttccaa
    19981 tagttgttaa aggtttttta ttttttatag tattttttgt tatttaagaa aggaataagg
    20041 ttaaacaaat aacaggttgc tttttgggtc tttttccgtt caagaaactg aacgaagaaa
    20101 agggaaagtt gtctcttttg tgataagaga aatcataata aacgaatgac ttactttcca
    20161 ataaattgag aacagtcaga accagacaaa ttagttgcat ctgatgaata aagtgattat
    20221 tctatgacat gaggtgatac agatgaaatt agaagtgaca aaagaagcac aagaagtatt
    20281 gaagaataaa ttagaagcag acgatcaact tttattagat atcgaaaacg gtgacggtcc
    20341 atttgcagaa agccaagtca gttgccaatt ggatacggca tttcgattga tcattgtcaa
    20401 aaaagataca caaacagatc taagtcttta ttctgtagtg gctgatacac cagttggacc
    20461 aatcaaaatc aaagacagtg caatacttta catggatgat ccatccacac ttgctttaga
    20521 accaacttat aatagtttac aattaaaagg accaagcggc ttgagaaaag gcaacttgca
    20581 agttgttcga aaagactaga gggataaaat gggaaaattg gcgatcatca gcgacctaca
    20641 tgtggacatc aataaattcg gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa
    20701 gcacaagatc actcggcttc atctagccgg agatacggct aataaagtag accgttgcct
    20761 agctgttgtc gaatttttcg tcaataaagg attatctacg acatttcatt tcggcaatca
    20821 cgagttggca gatgtgccca atgaacagat gatggaccat tttccagacc cacatttttt
    20881 aaatgaacgg tatattgctt taaatgagca gaccgttctt ttagggatga atggctggta
    20941 cgactatcag ttttccgaat tgaaagatta cgaggcaatc gtacgaatga agcgtttgta
    21001 ttggtatgat cggttcatcc agcgtgggaa aacagatctc gaggtcaatg cagaaatgct
    21061 tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg tgattttatc
    21121 cactcatttc gttcctaaac gggcatttat catgtatcaa tctgctcctt atgagcgttg
    21181 gaacaaatta aatgcatttt taggatcaga atcattcgga gaattacttg accgttattc
    21241 aaatatcaaa caagtagttt ttggccatac gcatcgtcgc tttgaagata aatccatcgg
    21301 tcacacgatt tacagctgca gaccttttgg ttattattat gaatggcagc tgacccgaga
    21361 ttttgtcttg aaagaacagt tgctagacag ctatgatcct atgaaattac gtgtcctctt
    21421 gcgtgcccat cacccagctt ttcaggccta tcaaacacaa catttgcaag aggaatttga
    21481 acaagcgatg acaatcgttc cgtattgaga cagcattagt aatgtgtaaa agaaaatacc
    21541 ttgtaaaaca taaggataag taagagcagt agatccattt atcgtggata tgctgctttt
    21601 tttgcggaaa ataatcagcg aatgtttatt tgatcggatg atttgtaact cgcagagtac
    21661 tagatgcaag cgtgtattct ttttttgaac gataatcaca aaaaaaaata taatatacat
    21721 tttttattta attataatta atattgattg aagtctaggc agtcaagtca cattttgata
    21781 gataggaggg ggacttacga attattttaa aggctatgac agctgtattt ctagattgtg
    21841 tttgcatcaa gttgcaataa aaactagaaa tcaaatcggg ctgataatca tttataacta
    21901 atcgatacat aggaccaaat ataggtctat gttcttttat ttcataagta tcgtaaaaca
    21961 gaattttgct atggattaat ccaatcgact gatatgttta gtaaataaag aaagaggaat
    22021 gtcaatgatg aaaaaagaaa tcgtattaac agcagcaatg atgttattca gcatggtagc
    22081 aagtacgact tttgtttctg caactgaagt atacccaaag gaatataaca cagaaggaac
    22141 gattacattt gaagcaggag atgagggtgt aactcctcct gttgatccag agaatcctga
    22201 tcccaataaa cctgtcgatc catcagatcc tccaagccct ggaacaggag gggcattatc
    22261 gattgattat ggatcaaaat tcaaatttgg gactcaaaaa atttctactg cggataaaac
    22321 gtattatgca gctgctgatg tgatgaacga tgggtctaga aaaccgacat atgtccaagt
    22381 gacagatagg agaagtacct tatctgggtg gaaattaagt gtctctcaac cagaacagtt
    22441 taaaactgcc agtggggatg aactagttgg agcacagctg aaatttacga aagggcaggc
    22501 tgtttctttg gttgatccaa catatacgcc gcaaacggtg aattctgaat taacgttgac
    22561 accgggagga aacaatacgt tagcaattaa tgctaaatca ggaacaggtg tggggacttg
    22621 ggtctatcga tttggtgcaa atgaaaatga aaatcaagat gcggtacagc tttcggtacc
    22681 cggtaaatct gtcaagttag cgcaacaata ttccactaaa ttagtttgga cattagaaga
    22741 tacacctaac aactagttta ctgaaaagaa ctgttaaaaa taaatacgta gtcatattgt
    22801 cttgcgaggt aatcgagcaa aaattgtcaa aggctaggct aaatttgttt ggatacgaat
    22861 agttcatcta ggaatttaac gatcaggagg aaaagatata ttgaaaaaga ggaggatcgt
    22921 gctcatttta agcagtctct tagtgactaa tatctttccg atcatagggt actctttgga
    22981 attgaatcag ttggagcatg gaaatagaag agacgaagaa agcaaactca cttcgacatt
    23041 cgacagttta atagaatcca accagacaca agaaagcagt gaggatttaa gtatttcgag
    23101 tagtggagag accactgaca acgtagaagc tgagggttcg gacaaacaag gactagaaga
    23161 tgcaccaatt tctgcggttg agctgcctga cgatcccaat attatttcta tcgataaaat
    23221 ttttcaaaca cctatcggta ctagcacgtc catattggaa aatgggaagt tactgcagtt
    23281 gaacccagca gaaaggtctc aaagaggagc tatttggtca aataagaaga ttagtttact
    23341 ctcggatttt acatttaaat cttatttata cttaggaaat caacgagata gagcgggaga
    23401 tggcatgacc tttacattga ctggcgatcc tagaatggct agtaatccca gtcaagttat
    23461 tggatcgcca ggtatgggaa ttggctcata ttctactcgt tctggccaac cttacattgc
    23521 gaatgcgctc tcgattgagt ttgatacgta taaaaatagt ggttcttctg atcgaatgga
    23581 tcgagagata actgctgata atagagggca tggacatgtc gcttttgtca cgcctaaacc
    23641 aaataataat aattattctg gagagcatac tggcgtgacg gtagctccta catttctttc
    23701 taatggcaca tggcgcttgt taataattaa atgggacgcg gagcaaagaa gtcttactta
    23761 tgatttggaa ggtgtcggtc gctcttctcg gattatacct gatctcatta ggcagtttgg
    23821 ttctacagaa gtaaattggg ggtttacttc ttcaacaggt actttttttc aagaaaatgc
    23881 tgtcgctgtc acgcgacttc catcgaatgt cacatcaaca gctgatgtaa aagtcaacga
    23941 tagtgattat ggggccatcg ctgaagctgg aggaaacgat aagataacta tcagaaatac
    24001 attgaaggtc gagggggatt ttcttgaata taagaaaatg cagactagta tcgacctccc
    24061 acaagaatta actattcccg agcatatctt gatcaatgga aatcaaattg atgccaaaga
    24121 tatcttgata accggaaatc gagtcacgct aaatctgaca gattatttta ttgctggaga
    24181 tacgttaata atcgaattgg agacaacttt gcaggaagta attcctgaaa aagtactcac
    24241 tatgcatttt gaataccttg aaaacgggaa tttgattgag caatcgaatg aaataaacct
    24301 aaagatcgca aagctgaagg agatagatat ccatgtttat tataaagata aagacagtca
    24361 gattgatatt gctgaaatga aaacaatcac tgggaaaata ggagagaagt ataaagaaac
    24421 tcctattgat attgaagggt atgtttttga ctcggattca gcgaatacag aaggcatctt
    24481 ctccgaaaat acagaggata tctattttta ttacagaaaa ggcaaacttt atctctcaac
    24541 tgctcctcaa tatgtcaatt ttggaaaaca taaaatatcc agtagaccat taagtgaatt
    24601 cggacagttt tcaggtgggt tgaaaattat agatgaacgg gcttttggaa gttggaaatt
    24661 acaattgaaa caagagaaag ttcttacaaa tggagatttt gagcttccag aagctctttc
    24721 ctttgttacc tctgaaaata caacggttat agggacatcc gctgttacga ttttcgaaag
    24781 tgatcaaaag ggtgaaagcg atttgtcctc tcttttagat tctacagctc atcgtggaat
    24841 cagaataaat attcctgtag aaaaccaaag aatcggaacc tttgagggga aactatcttg
    24901 gatacttgca gatgtcccag gaaattaaca ggaagagaag aatataacaa aattattgag
    24961 tttcaagaag ggcacaggta ccataggctc ttgtgtccca cagagacaag attttgttat
    25021 agtcttctct tacatatgat taaaggagcg attttcttga gaccaaaaca acaattattt
    25081 ataacactcc tagttatttt aggggcactt tttaacaata tatcagtgtc agctagtgca
    25141 tttaattttt cagtgacgcc gataacttca gagaatcaaa tcgacaagag aaaaacttac
    25201 ttcgatcttc aacttgatcc ggatcaggaa gtagaagtaa aagcggaatt gagaaatgat
    25261 acagaaaaag aagtgaaaat cgatatttct gtgaatagcg caaccaccaa ttcaaatgtg
    25321 atggttgaat acggaaaaaa cgagattgaa aaagacatga gcctgatctt tgatctggta
    25381 gattatgtga gctatccaca gatagtgacg ctaaaaccta aaagtgtcca aaccgttgct
    25441 tttcatgtca ggatgccgaa tgagcgattt gatggtgtat tggctggtgg gatcacattc
    25501 caagaaatcc agacggaaaa ggaccaaaca gaaactaagg atcaaagctt atcgattgag
    25561 aatgaataca cctatattat tgctttgttg atgcaacaga cactgaacga agttgctccg
    25621 aaccttactc ttcatgaagt taaaccagat caaatcaatg caagaaatgt catcttagca
    25681 aacgttcaaa atgatcaaaa aacctatatc aatcaagtag ttattgaaac aaaaattaca
    25741 aaaaaaggac attctgaagt tttatatcaa gaggaaaaag aaggattgca gattgcgccg
    25801 aatacaaatt tttcttttcc aactgcttta aatggtcaac cgctaacccc aggtgaatat
    25861 caccttacaa tgactgtttt aggaaatgaa aatgaaaatg gaaaattttc gagaaaaaaa
    25921 ggaaatacta cgatcaacta taccaatcaa tgggtatttg aaaaagattt tacaatagac
    25981 ggaaaagtag ccaaggaatt gaacacgacg gatgttacga ttaaaaaaga caataattgg
    26041 atttatatct tagtagcttc acttttctta ttgtttattt tattatgcat acttttgttt
    26101 gttttgagaa aaaaagaaga gaaagaaaag taaggttaag ataacaaata gtaaattttt
    26161 attattgtag gatggttggg ctctttttat aacaatataa aatataccat tttattaata
    26221 aaaatgcata ctagtaaatg atataattct aataacaggt ttgatcgcga gaaaggccaa
    26281 ataaaacgct ctccaaaatg ttaaaaaaaa gacaggacga tgtatattat acaaacaaga
    26341 atcgctgaca acagtgactc ggatccagga aatcagtttt atccccagct agttggtgaa
    26401 tgcatggaaa gtaatatacg tagaaagagc tgatgactaa tgaattatga gtttttttta
    26461 agcaaagaag atcacagaag ttattcatta cttaaatatt tagaagcaag ctcaaattta
    26521 tcagagtcaa tatcgaatgt tcaagaagag ttgtcgctat cgacatttct cttgaaaaaa
    26581 acgatcgata agctgaaaca tgatttacaa aagttcgatc tagagaaaaa tttgcgagta
    26641 accgtcagtg aattggatat atgtctggaa attaatggaa aatgttctag caaagctctg
    26701 ttatctcgtt atataacgga ttctttatct atgaaaatgg tattggcttt ctttcaggaa
    26761 aaatatatct cagtagaaaa ttttgctaaa cagcatcacg tgagttattc tgtggcttat
    26821 aaagtattac aaagactaaa aagaaatcta aaaaaatatc aaattttttt tgaaaagaga
    26881 aagtt
//
LOCUS       DAYYCQ010000035        26741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000035 DAYYCQ010000000
VERSION     DAYYCQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.035"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1385)
                     /locus_tag="ACHAYF_001438"
     CDS             complement(<1..1385)
                     /locus_tag="ACHAYF_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8578880.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTL"
     gene            complement(1396..2064)
                     /locus_tag="ACHAYF_001439"
     CDS             complement(1396..2064)
                     /locus_tag="ACHAYF_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8578881.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2194..2778)
                     /locus_tag="ACHAYF_001440"
     CDS             complement(2194..2778)
                     /locus_tag="ACHAYF_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8578882.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3109..3342)
                     /locus_tag="ACHAYF_001441"
     CDS             complement(3109..3342)
                     /locus_tag="ACHAYF_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8578883.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3357..4307)
                     /locus_tag="ACHAYF_001442"
     CDS             complement(3357..4307)
                     /locus_tag="ACHAYF_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8578884.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4304..5146)
                     /locus_tag="ACHAYF_001443"
     CDS             complement(4304..5146)
                     /locus_tag="ACHAYF_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8578885.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5148..5867)
                     /locus_tag="ACHAYF_001444"
     CDS             complement(5148..5867)
                     /locus_tag="ACHAYF_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8578886.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6415..6717
                     /locus_tag="ACHAYF_001445"
     CDS             6415..6717
                     /locus_tag="ACHAYF_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578887.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            6789..8015
                     /locus_tag="ACHAYF_001446"
     CDS             6789..8015
                     /locus_tag="ACHAYF_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8578888.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8103..8495)
                     /gene="rpsI"
                     /locus_tag="ACHAYF_001447"
     CDS             complement(8103..8495)
                     /gene="rpsI"
                     /locus_tag="ACHAYF_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8578889.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8511..8939)
                     /gene="rplM"
                     /locus_tag="ACHAYF_001448"
     CDS             complement(8511..8939)
                     /gene="rplM"
                     /locus_tag="ACHAYF_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8578890.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9473..9940)
                     /locus_tag="ACHAYF_001449"
     CDS             complement(9473..9940)
                     /locus_tag="ACHAYF_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8578891.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10060..11598)
                     /locus_tag="ACHAYF_001450"
     CDS             complement(10060..11598)
                     /locus_tag="ACHAYF_001450"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8578892.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11678..12364
                     /locus_tag="ACHAYF_001451"
     CDS             11678..12364
                     /locus_tag="ACHAYF_001451"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8578893.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12565..13092)
                     /gene="msrA"
                     /locus_tag="ACHAYF_001452"
     CDS             complement(12565..13092)
                     /gene="msrA"
                     /locus_tag="ACHAYF_001452"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8578894.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13176..13853)
                     /gene="pgmB"
                     /locus_tag="ACHAYF_001453"
     CDS             complement(13176..13853)
                     /gene="pgmB"
                     /locus_tag="ACHAYF_001453"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8578895.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13850..14980)
                     /locus_tag="ACHAYF_001454"
     CDS             complement(13850..14980)
                     /locus_tag="ACHAYF_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8578896.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14980..17163)
                     /locus_tag="ACHAYF_001455"
     CDS             complement(14980..17163)
                     /locus_tag="ACHAYF_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8578897.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17160..18185)
                     /locus_tag="ACHAYF_001456"
     CDS             complement(17160..18185)
                     /locus_tag="ACHAYF_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8578898.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18230..19057)
                     /locus_tag="ACHAYF_001457"
     CDS             complement(18230..19057)
                     /locus_tag="ACHAYF_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8578899.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19054..20121)
                     /locus_tag="ACHAYF_001458"
     CDS             complement(19054..20121)
                     /locus_tag="ACHAYF_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8578900.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20147..20983)
                     /locus_tag="ACHAYF_001459"
     CDS             complement(20147..20983)
                     /locus_tag="ACHAYF_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578901.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20980..21849)
                     /locus_tag="ACHAYF_001460"
     CDS             complement(20980..21849)
                     /locus_tag="ACHAYF_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578902.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21919..23202)
                     /locus_tag="ACHAYF_001461"
     CDS             complement(21919..23202)
                     /locus_tag="ACHAYF_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8578903.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23216..24901)
                     /locus_tag="ACHAYF_001462"
     CDS             complement(23216..24901)
                     /locus_tag="ACHAYF_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8578904.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25176..26201
                     /locus_tag="ACHAYF_001463"
     CDS             25176..26201
                     /locus_tag="ACHAYF_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8578905.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         8022 a         5393 c         4458 g         8868 t
ORIGIN      
        1 atcaaagttg aatcttctac tctaagtttt atatcttttt ttattccacc attttttaaa
       61 taaataattc tattgcatgt tttataaaga aattccggat catgagtagc tataactact
      121 gcaccctttt cttttaagtc tttaataagg tcagcaatcc tttccatatt aatataatcc
      181 aacccacttg taggttcatc aaatagaaat aaattagttt ttgacaatga agctatagaa
      241 accaataatc tttgtttttc tccaccggat aaatcaaacg gtaattgatc tttcatttta
      301 taaagttcca ttttttttaa aattttttct tgctgatctt tactaactat ttcattcccc
      361 agggctaact cgctcctcac tgattctgtg aaaaattggt aatccatttc ttgcattact
      421 aaaaacggcg atgacaaatt ttttttattt tcgattatct ttcctttggt agggcgaata
      481 atcttcatga gtattcttag taaggttgtt ttccccacac cgtttttccc taccaaacca
      541 ataacatctc caactttgac atcaaaattt atgtctttta gaatattttc ataagtaatt
      601 tcttcaactt ttaatacatc tgcacttacc ttttcgatat agggaacttc aatcgaacta
      661 ttaaaaatgt caaatttcct taatgcgtta ttgttttcag tttttaacaa ctgaatagtt
      721 gtagttttta cagttctatt tttaacaaat agaacttgat caattaattc tttcaaatag
      781 taaaaacgat gttcagctat aacaatagta aatcctttat tttttaaatc acaaagtaag
      841 tcagcaagtt tcttagtcgc tttgaagtcc aaatttgaag acggttcatc caatataatt
      901 aatttagagt ttagcgataa actacatcca atagaaattt tctgccgttc accacttgat
      961 agcgaaaaaa tgtttttatc taatatattt gaaagatcta ataattcagc taattcattg
     1021 attcttttat ccatatgctc ttttgtaaat ccaaagtttt ccatcgaaaa tactaactca
     1081 ctcgttgtat ttgttgtaaa gaattgtcct ctaggatctt gacttacgta gccaatattt
     1141 tttgatatac tttcttgtgt taaggattgt agcgagttat tttcaaataa aatttcccca
     1201 accaattttc cgtcataaag tcttggaata agtccattca ttaactttaa tagtgtggat
     1261 ttcccactcc cagaagaacc agtaatcaat gtaagactgc ctttttttat tgaaaaatta
     1321 aggtcaacaa gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt gttaaattca
     1381 aacaattctt tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg aaaacaggat
     1441 gatatagtct aataagctca tgtttgtttt atagtaagaa cttcttttca caggagattc
     1501 tatccccttt gttattgcat tagcggtcat ttcttcagca atcatagttg cacgtataag
     1561 aacgggaact aacagaaatt ccaaatattg cagaggctga aaaaaagaca agcgaatgtt
     1621 tctcattttt aaagattctt taatcattct tatttcagaa cgataagtcg gaaagaatct
     1681 gatcactact gtaatactta acattatttt tttcggtaaa tgtatttttt ctaaagagga
     1741 taataattca ttgctagtat agacactaac caacaaccat gagatcatta cgaccggcaa
     1801 cattcgaaat acaatatata aggatgtcgc aaaaaatata gtaaaaaact ctatcttgat
     1861 agcatcaata attttcatta ctaaaaacag aactgaataa atcaagatga aataaataca
     1921 tgtctttatt ttacgaaaca tcaatactaa aactgtagca ataatgaaac accccacttg
     1981 aaatatattt ccctttccta aaaaaataat gaccggaaaa aaaatattta aagtaatcaa
     2041 ggttaacgga ttgatttttg acactctttc acctccaaaa aataagatcg cactgttatc
     2101 ttaagctgat aaaaatgcac cgttattcag ataacagtgc gatcttatca aaaagcataa
     2161 ctgagaacaa ttctcaatgc aagctttttt taattaactc agtaattcaa tccacccagg
     2221 aacagaaaat tgatacaatc ttctggttaa tagcatagtc ttttcttttg aatagctatg
     2281 cttcaatata tcatagaatg tagcgtagaa tgagtaaatg tagaagtgga tttcttcttc
     2341 attaaagact aaattttgat tgatcattcc gatagtcttt aacttatcta gacaacagac
     2401 tgtctttttc gtcacaaatg atgtaatttt ttcaacaata tcttgatatt tggttccagc
     2461 tgattggaat aaaatcaaat cgaatatacg tttattctca aaaatataat taataaatgg
     2521 atctaattca ttgaagttct ctctccaact tttctctata tctatatttt ctaagtttaa
     2581 gaggtatttg gtaaactctt cattatacat attaataagc gcattaatat gcggctgaat
     2641 aatttcttca aataacgctt ccttagaagt aaaatgttta taaaaagcac ccgtagttac
     2701 gcctgcggtc aggcacagtt gccttatctt agtttgctga aatccttctt tttcaaaatg
     2761 gcttagtcca ctttctatta acttttggtg agttataata taatctctgg tttctatggg
     2821 tacttcctcc taataagatg aattaacttt attatcttac acaggacttg aagtttcaat
     2881 cgttaacgtt tattactgaa ctcattcctt agagctaaac taatgagaaa gagcagaata
     2941 actgatacaa aaagcaatcc tacaagaatt aatagactca tctaaatcat tcaccttttc
     3001 taaaataatc cgagaaagat ttaaaaaaat tatatccagc tattaggata agtcctatca
     3061 taatcactcc aatagccatt ttcttatcct ccctaaaaat aaagtctttt acttaaaacc
     3121 ttttttgtaa tcgtcaataa attttttccc tttatcagtt aattgaaatc ctatatctaa
     3181 atagtcctca tatctaagtt tagccagcat ctcacttaca gatgctggac taacacttag
     3241 aacttttgct aaatcattat tttttaattt cttttttctt tttgataatt cctgaattgc
     3301 tagtaaataa attagtatag tagtcgtttt aatttgtacc atacctaccc tccaaattat
     3361 ttcgaaaggc cttcagcaat tttatccagg ttccatttca tcatcgcata gtaactatca
     3421 ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg ttgaatagat aggaatattg
     3481 gtatcttttg atactgtttt cattggtcta tcatccacac tgctttctac gaataaggag
     3541 ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta tttgatctgg tgttccttct
     3601 tcttcagtgt tgatttccca aatgtaagca gaaggcacat tatacgcttt cgagaaatat
     3661 ttaaagcatc cttcacttgt aacaatcatt tttttatcat tcggaattga agcaaattta
     3721 gatttagctt ctttgtctag agaatccaat ttttcaatat acttatctag attttcttta
     3781 tagaattttt tattatcagg atctttttcc gctaactgtt tttcaatatt tttagcgtaa
     3841 ataataccgt tttctaaatt taaccaagca tgaggatctt ccttcccttt ctcactctga
     3901 ccctctaagt aaataacatc tatgccatca cttgctgcaa aatagtcttt gttttcctct
     3961 ttgttcgcat ttttaactaa ttttgtaaac caagcatttc ctccagtttc caagttaaca
     4021 ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa catcttcagg caaaggttca
     4081 tattcgtggg gatctttccc aataggtacg atactgtgta aatcaatttt atcttttgct
     4141 atattttcgg taatatctgc taaaatcgaa ttagtaacta ctacttttaa tttttcgttc
     4201 gaagccactt tatctttgtc tttactattt attgaacatg ccccgaatat aaaaagagca
     4261 atcaaaatcc ccagacttcc cagtactgct ttccattttc tcatcataac gatctctcct
     4321 tctttcttaa aaatttttgt tttggtgaga ttagaaaact aattacaaat aagaatgccg
     4381 ctgttaaaac aatacttgat cctgctgcta tgttaaaact ataaccaata aacaatccaa
     4441 gcaatgatga acttgctccg actgttgcag aaataaatat catagttttc aagctatttg
     4501 catataaata tgctgtagct gctggtgtaa ttaacattgc aacaattaat atagttccca
     4561 cactttgcat agctgttact gctactaaag ttaatagaac catcaataga taatgataaa
     4621 aactgacatt catccctatt gcttttgcca ttaatggatc aaaagaagtg attaataatt
     4681 ctttaaaaaa tagaactact ataaccaaca ctaaaaccga aacgccaatt gtaatccact
     4741 tatcaatatc ttgtacagct aaaatatttc caaataaaat atgaaataaa tccgttgagc
     4801 tatttgcaac tcctataagg ataactccta gtgctaaaaa agaactgaaa gtaataccaa
     4861 ttgctgtatc tccttttatt acactattgc tttttataaa agtaataatg atagaagcta
     4921 atagaccaaa tacaatcgca ccaataaaaa aattaatacc taaaatataa gaaattgcta
     4981 ctcctggcaa aacagcatga gaaatagcat cacccataag agacattcct cttaaaataa
     5041 taaaacaacc tactgttcca gaaacaatcc ctataactac agatgtaatt agagcattct
     5101 gtaaaaagtg aaagtcatac agaccattaa taaaattttg aagcatatta ttccccctct
     5161 ccaataaata tagtatctcc ataggctttt tttaaatttt cttcattaaa tacagattct
     5221 acacttccgt gagcaattag atttcgattc aaaagaataa tattatcaaa atacttcttt
     5281 accttgctta aatcatgatg cacaattagc actgtttttc cctgatgttt caattttttt
     5341 aacgtatcca taattattcg ttcactaact aagtctatcc ctacaaatgg ttcatctaaa
     5401 aatatgaaat ccgcattttg agcaagacat cttgctaaaa gtacacgttg aaattgtcct
     5461 ccagataatt ctcctatttg atgattagaa tattttccca tatcgacctt ttccaaagcc
     5521 tttgaaactc tttgccattc tgcattttta attctttgaa aaactttcat tccggcatat
     5581 gttcccatag atacacattc ttttactttt attggaaaag taaaatcaat atcagctttt
     5641 tgctctacat atgcaatctt caccagtttt ttattgatat cttcattatt aatggagacc
     5701 tctccctgat gagggataat atttaacatt gctttaagca atgtggattt tccagcacca
     5761 tttggaccaa taatacctgt taaagcacct tcttgtattt ttaaagaaat ctcgttcaat
     5821 gctaaaaaat cgttataaca aacattaagc tttttcactt ctaacataac tttctccttc
     5881 taaatgataa tgattatcac gatcatatgg tatacaaaaa ttatatttta gtcaacccta
     5941 aattttatag tttagatttt tttctgttgc tgaagattag aatgacaaaa ctatttgaaa
     6001 ctacgcgtat agtaagtagc tttcaaaata tataaaaaag gagcgttctc aaatgagaac
     6061 atgctcctaa aactacttta ttggcgtatg gaaaaattat acttgaagtg gatagcgggt
     6121 tgaataagtt gataagcatc aaaacattta taaaaattat tcaagactat gtctgttcca
     6181 tatacaaata tgaaaatcag taaactccat ttaacttttt tcgtagtgtg tctttttata
     6241 catgtttatc gttattaata gtgaatagga aaaaaactat ttttgatcat tgacaattaa
     6301 atacaatttt tgaataacga tccctgctat tcgagctaga aactcaagta cgccatgacc
     6361 tgattagcga caaatactta gagatttctt tttgttctgg gaaaacaaaa tgccatgaca
     6421 atagcagcaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc
     6481 ctatgtgctt acccaaagca attcaaaaat ttcataacta ctattttagg agatgatttt
     6541 atagaatcca gagtctacct gaataaaaaa caaacctgtg catttctagg aatcgataat
     6601 aatactctag ataagtggat tcgtatatat aatttcccag taataagagt tggtggtgta
     6661 gttcgctttg acgtagaaga tattaaagac tttatgaata catttaaaat aacttgaaat
     6721 tttcttctcg ccgcatatac taaaattagt gaaatatact gccttacttt ttttaaggag
     6781 tgaggatttt gcctacaaag ctttcaaatg ggaaatataa aactaatctg agatatccaa
     6841 aaaggtttaa agaaataaca ggaattgcat cagaaaagta tcaaaaaaca tttccaaata
     6901 ggcaattggc gattaaagcc gaaaatgata tgaagaagaa aatagaaaaa gttttgagag
     6961 aggagaatgc aaactcatta gaattaaaag gaaaaataac gtttaaaaaa ttttacgaat
     7021 ctaaatggct tcctagatat gaattaggtc aaacaattag atcaaacagg cctcctagtg
     7081 atataaccat tagtaataca aaagatatat ttcggttgca tattttacct atgtttggtg
     7141 aatacgcaat gaattatcta aatatcaata ctgagataat atcagatgaa ttaaccaaaa
     7201 aatcgaaaga atatgcaaat ataaaaatca taaaaggata tgttcgttcc atgttcgata
     7261 ttgcagaaat actaaattat atcgaattta atcgaactac taaaattatt caaagtatca
     7321 ctgcacctaa aaaaaatgct ttagaagaaa aaagaataca agaaggtaag caagcactat
     7381 catcaaaaga acttactaat tggatagaag cagtaaatga tgattttaat aatcatttac
     7441 ttacttttca tgattacaca ctttttatga ttaccctgta tcttggtgat agaaaaagtg
     7501 agacttacgc tttgcaatgg aaatatattg attttgaaaa acaaacggtc cgtcttaaac
     7561 atactttaga taaatatcaa agaaaaaagt ttacaaaagg tcgaaaagat accgtcattc
     7621 aagtaccaga agttgtaatg acattgctaa gtgaatggaa atcggtacaa gctgatcaat
     7681 tgttgaaatt aaaaattaag caaacgttag atcagtatct ctttacttat actaaaccat
     7741 ctggagaagt aaattgtcct gttcatgccg attacctaaa ctatcgaatc aattcaatta
     7801 aaagaaggca tcctgactta gttcatttat ctccacacaa actccgtcat acttatgcaa
     7861 ctatagctag acaaggtgga gcagatatga atcaaatttc aaatgcttta acacactcag
     7921 atatttctac aacaaagata tatgtgaaca caccagatat agttgataag gctgtttttg
     7981 aagcttttca gagaggattg aataagtgtg attgatatgt gttcttaaat gtgttcttaa
     8041 aaacaaaaga agcactttcc aaataattga aaagtgccag aaacgttgat atataaagga
     8101 tattaacgtt ttgagaattg tgaagcttta cgtgctttct taagacctgg ttttttacgt
     8161 tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca
     8221 ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg
     8281 tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg
     8341 aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt
     8401 ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta
     8461 cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag
     8521 tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat
     8581 acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca
     8641 gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc
     8701 aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt
     8761 aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg
     8821 aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat
     8881 ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata
     8941 tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga
     9001 gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt
     9061 caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt
     9121 cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa
     9181 gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca
     9241 agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga
     9301 gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat
     9361 tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag
     9421 gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt
     9481 cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg
     9541 ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga
     9601 ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt
     9661 ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt
     9721 cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta
     9781 actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg
     9841 gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa
     9901 ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt
     9961 ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg
    10021 agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa
    10081 ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac
    10141 aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc
    10201 tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca
    10261 gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta
    10321 acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc
    10381 acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag
    10441 acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc
    10501 gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg
    10561 atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc
    10621 atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc
    10681 tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg
    10741 atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa
    10801 acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa
    10861 ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa
    10921 acctagttct gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac
    10981 ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa
    11041 ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc
    11101 aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa
    11161 ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg
    11221 cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc
    11281 gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc
    11341 caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga
    11401 aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg
    11461 atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc
    11521 tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa
    11581 tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta
    11641 agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg
    11701 ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat
    11761 tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat
    11821 tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc
    11881 cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt
    11941 ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt
    12001 gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt
    12061 atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat
    12121 cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt
    12181 gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc
    12241 tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga
    12301 aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta
    12361 ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc
    12421 aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca
    12481 atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt
    12541 tccataagta gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc
    12601 tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc
    12661 ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg
    12721 cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc
    12781 ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc
    12841 taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat
    12901 aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac
    12961 ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat
    13021 ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc
    13081 tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga
    13141 taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat
    13201 aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca
    13261 gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct
    13321 tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc
    13381 tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca
    13441 ataaggacat caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg
    13501 cttgctgatc ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt
    13561 gcaccaggca aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt
    13621 ttttgctcag caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat
    13681 gacagcaatc gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc
    13741 tcagtgaatg gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga
    13801 gctgtgtcta caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac
    13861 ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat
    13921 tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa
    13981 cggccgaaat gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag
    14041 ctatcacagg aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta
    14101 gactttgtcc atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt
    14161 tatcgaactc cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa
    14221 atgctaacag tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt
    14281 gaggggcaaa tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt
    14341 ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat
    14401 tagtaacatc agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt
    14461 tatcaactgt agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt
    14521 gatcgtaaaa agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac
    14581 ttccgccaac gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt
    14641 ctcctactcc tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca
    14701 atccgacgat ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt
    14761 ctttgttttt gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt
    14821 ctgctaatga tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag
    14881 cctgaacaaa aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca
    14941 gactttcttt gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa
    15001 gatcagttct ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt
    15061 cgtctctttt tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt
    15121 ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag
    15181 agttccattc gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc
    15241 taatgaagca gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag
    15301 gcacgcttct tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat
    15361 ggcgtgaatc gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa
    15421 attcttagaa acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag
    15481 atctgcttgc ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat
    15541 agactggctg ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata
    15601 cgtgtcatct tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa
    15661 aaaattttga cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc
    15721 cacattataa tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc
    15781 atctggacca atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag
    15841 ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc
    15901 ctctgttgct tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc
    15961 tgcggcagct gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt
    16021 gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg
    16081 ataatctctt gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt
    16141 ctttgctact tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca
    16201 gaaaacatgc cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg
    16261 atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc
    16321 gaaagaagaa tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga
    16381 catcgactgc tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt
    16441 cttaaacggg tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa
    16501 ggacgtattt tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg
    16561 gaatacacct gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt
    16621 ctggtaggat gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg
    16681 ctgtgtacca aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc
    16741 ttcggtcaac ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc
    16801 aacacgacaa gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg
    16861 tttaagctct cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc
    16921 aagagaaaaa tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac
    16981 taactcggct gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct
    17041 tacttgattc cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat
    17101 ggtcatctga ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt
    17161 cataaggtaa taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg
    17221 atgacatatc cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc
    17281 acaaaatgag ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa
    17341 accagctttc catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga
    17401 tcgccacaaa aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc
    17461 agttggatga tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg
    17521 aatttgcttg gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg
    17581 aagctgtacg cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca
    17641 agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac
    17701 gttccccagc ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc
    17761 ccgagaacat caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat
    17821 ccataagcca aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca
    17881 gctgtcatag ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat
    17941 acatgttcga agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca
    18001 ttcagcattt gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct
    18061 tctgctcgct ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt
    18121 tgataataag cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc
    18181 ttcatttatt tctccctcct ttgcttattt acttattgtt attctttctc tattctttct
    18241 ctaaatcatt taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct
    18301 gataaatttt cacttcattt tctcctcgca aacgacattc atataccata taaccttcat
    18361 actcattttg aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt
    18421 ggtagcgatg attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg
    18481 cttttgaaat atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat
    18541 gtttcaattg attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt
    18601 cttggtatcg gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat
    18661 ctaaataacg aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa
    18721 ccatcggcgg taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc
    18781 ctccaacctc gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc
    18841 gttcttcgat aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga
    18901 tccctgtttc tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt
    18961 cgatttcgta ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc
    19021 ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac
    19081 gcccattttg atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct
    19141 ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa
    19201 taattcccaa catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg
    19261 ttcactagat gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag
    19321 attaaaccca gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc
    19381 gatccctctt aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc
    19441 tactccacgt ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc
    19501 tagcgtgtaa tcggcaccgt atttttctgc taattctcta cgctgcgcta tgggatcgat
    19561 cccaacgatg gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc
    19621 gcctaaaccg aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc
    19681 taaagcgaat tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag
    19741 tttacgtagt ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata
    19801 agtgcagact aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt
    19861 accgacaatc atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc
    19921 ttctcttggc aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa
    19981 tcctctaaag tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg
    20041 tacttcgttc ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc
    20101 taatgcaact aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc
    20161 cgccttcgac taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg
    20221 gtaaagcggt caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga
    20281 ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag
    20341 caaataaata atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag
    20401 aaatagccaa tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca
    20461 ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg
    20521 cagtaggaag tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac
    20581 ctagaccgga aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa
    20641 cggtgtaaaa gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt
    20701 atgccccgct gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac
    20761 tatttttgaa atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt
    20821 caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct
    20881 tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa
    20941 tcgtcgcgta aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt
    21001 tcgtgagagc aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc
    21061 ttttccaaga tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac
    21121 tttattcgtc agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac
    21181 aatcacagtt gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca
    21241 agcatttgca ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat
    21301 agccaaaaat gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat
    21361 aaagaagcta gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct
    21421 atctactaac agatcattga cgattccata actattattg aacatatatt tccaagcaaa
    21481 aatcaaagaa atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct
    21541 aaatctgaac ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc
    21601 tacaactaag aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact
    21661 taaaatttca agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg
    21721 catatcgaaa aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa
    21781 taaaagcatg cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg
    21841 acgattcatt gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac
    21901 cattggcaga acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac
    21961 atcttcgttt ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat
    22021 atctcccatt ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa
    22081 agcactagca atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta
    22141 accatccagt tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac
    22201 ccacttttcc atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt
    22261 ttgtttctcg tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg
    22321 atctttttgt gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga
    22381 aggcaaaatg taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt
    22441 gatttctgtc acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga
    22501 caatgcttct ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt
    22561 tgctccattt gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt
    22621 aggcatagca attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa
    22681 aatatcatcc caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata
    22741 ccaaatacct tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga
    22801 acgaacaagg tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt
    22861 ttggactgcc gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact
    22921 gacttccatt acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa
    22981 cgctgattct tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc
    23041 agcaaccagt tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg
    23101 aatcgttgtc tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa
    23161 tccagtagct agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt
    23221 ttattatttt cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc
    23281 aatggataat ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg
    23341 ctgattgatt ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa
    23401 aactgataaa agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt
    23461 tttctttttt tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg
    23521 gatctgtctg caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc
    23581 gattctctcc tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa
    23641 tataaattgc cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt
    23701 tgcttctcca ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta
    23761 tctcatatgg actggtcgtt ccatctgggt ttttcttgta gtttactcgg gtaccctctt
    23821 tcttcagctg ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat
    23881 tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga
    23941 aatcgatggt ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa
    24001 gggtcaaagg tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa
    24061 aataactgat attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag
    24121 ctgcttcatc agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta
    24181 tacaagaagt tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag
    24241 cgcctttttc taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt
    24301 taaaattcaa gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt
    24361 ttttgtcaat gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat
    24421 ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt
    24481 cctgaaacca agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg
    24541 tttcgcttct catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat
    24601 aatttacaac ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa
    24661 ctctaaatgt atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt
    24721 gctgctcttc aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat
    24781 gcagtttgcc tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag
    24841 aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca
    24901 tcggattccc ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg
    24961 cttactgtca atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa
    25021 gcttatgtat caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag
    25081 ctattcctaa attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat
    25141 tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg
    25201 cgaaaaaagc taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata
    25261 tctctgataa agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga
    25321 gcaaaattgc aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc
    25381 gtacttctca tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg
    25441 ccgtttctga acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag
    25501 agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa
    25561 gctctacgat ggatgaaaca cttctttttg aattggatca atacaatatt cctattgtgg
    25621 taatcggaaa agtcggaaac gactacacga acatctactc agtggacacg gataatttcc
    25681 aggatagtta cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc
    25741 tccatgctcc gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct
    25801 tatttgatca ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag
    25861 aagatagcat ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt
    25921 tttctaccga tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc
    25981 agatacctga tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt
    26041 cgccacagtt gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac
    26101 ttttattcca actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg
    26161 agattactct cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac
    26221 agaaacactg aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac
    26281 cccctcccgg tattttagcg aatttttctt attttccatc tgttttagat agctgtggat
    26341 aactataatg tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca
    26401 cgttgtgtat aagtttttat agttcgtttt tttaaacaca tttaccctta tttcaatcca
    26461 tgttatttgg ggatacttat ctctaaaaca taaatagaat aacagacatg tctactggtt
    26521 ttgttttatt cgaggaatta ttcaataata gctagcaaaa tattaaaaat aaccgctcta
    26581 tcttcccatc aatttatttt gcattcaaaa aatgatctat ctttactttt gttgattgac
    26641 ttctcttgga agatgatcgt tccgatacct taattggata cgaaccgatg ttgttaagat
    26701 cctttcccac ttttcttcta gttagacaaa ttattttaga c
//
LOCUS       DAYYCQ010000036        26369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000036 DAYYCQ010000000
VERSION     DAYYCQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.036"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..724)
                     /locus_tag="ACHAYF_001464"
     CDS             complement(<1..724)
                     /locus_tag="ACHAYF_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8578906.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEA"
     gene            complement(728..1117)
                     /locus_tag="ACHAYF_001465"
     CDS             complement(728..1117)
                     /locus_tag="ACHAYF_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578907.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1252..1794
                     /locus_tag="ACHAYF_001466"
     CDS             1252..1794
                     /locus_tag="ACHAYF_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8578908.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1942..2574
                     /locus_tag="ACHAYF_001467"
     CDS             1942..2574
                     /locus_tag="ACHAYF_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578909.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(2708..2833)
                     /locus_tag="ACHAYF_001468"
     CDS             complement(2708..2833)
                     /locus_tag="ACHAYF_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8578910.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(2967..3860)
                     /locus_tag="ACHAYF_001469"
     CDS             complement(2967..3860)
                     /locus_tag="ACHAYF_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8578911.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(4042..4968)
                     /locus_tag="ACHAYF_001470"
     CDS             complement(4042..4968)
                     /locus_tag="ACHAYF_001470"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8578912.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(5053..5814)
                     /gene="pflA"
                     /locus_tag="ACHAYF_001471"
     CDS             complement(5053..5814)
                     /gene="pflA"
                     /locus_tag="ACHAYF_001471"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8578913.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(6058..8289)
                     /gene="pflB"
                     /locus_tag="ACHAYF_001472"
     CDS             complement(6058..8289)
                     /gene="pflB"
                     /locus_tag="ACHAYF_001472"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8578914.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(8566..11016)
                     /gene="parC"
                     /locus_tag="ACHAYF_001473"
     CDS             complement(8566..11016)
                     /gene="parC"
                     /locus_tag="ACHAYF_001473"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8578915.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(11028..13073)
                     /gene="parE"
                     /locus_tag="ACHAYF_001474"
     CDS             complement(11028..13073)
                     /gene="parE"
                     /locus_tag="ACHAYF_001474"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8578916.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(13239..13673)
                     /locus_tag="ACHAYF_001475"
     CDS             complement(13239..13673)
                     /locus_tag="ACHAYF_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8578917.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            13800..14438
                     /gene="plsY"
                     /locus_tag="ACHAYF_001476"
     CDS             13800..14438
                     /gene="plsY"
                     /locus_tag="ACHAYF_001476"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8578918.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(14570..15445)
                     /locus_tag="ACHAYF_001477"
     CDS             complement(14570..15445)
                     /locus_tag="ACHAYF_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8578919.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(15527..16318)
                     /gene="codY"
                     /locus_tag="ACHAYF_001478"
     CDS             complement(15527..16318)
                     /gene="codY"
                     /locus_tag="ACHAYF_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8578920.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(16336..17736)
                     /gene="hslU"
                     /locus_tag="ACHAYF_001479"
     CDS             complement(16336..17736)
                     /gene="hslU"
                     /locus_tag="ACHAYF_001479"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8578921.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(17750..18298)
                     /gene="hslV"
                     /locus_tag="ACHAYF_001480"
     CDS             complement(17750..18298)
                     /gene="hslV"
                     /locus_tag="ACHAYF_001480"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8578922.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(18335..19240)
                     /gene="xerC"
                     /locus_tag="ACHAYF_001481"
     CDS             complement(18335..19240)
                     /gene="xerC"
                     /locus_tag="ACHAYF_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8578923.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(19438..20814)
                     /gene="trmFO"
                     /locus_tag="ACHAYF_001482"
     CDS             complement(19438..20814)
                     /gene="trmFO"
                     /locus_tag="ACHAYF_001482"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8578924.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(20783..22861)
                     /gene="topA"
                     /locus_tag="ACHAYF_001483"
     CDS             complement(20783..22861)
                     /gene="topA"
                     /locus_tag="ACHAYF_001483"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8578925.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(22983..23840)
                     /gene="dprA"
                     /locus_tag="ACHAYF_001484"
     CDS             complement(22983..23840)
                     /gene="dprA"
                     /locus_tag="ACHAYF_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8578926.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(23896..24663)
                     /locus_tag="ACHAYF_001485"
     CDS             complement(23896..24663)
                     /locus_tag="ACHAYF_001485"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8578927.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(24656..25516)
                     /gene="ylqF"
                     /locus_tag="ACHAYF_001486"
     CDS             complement(24656..25516)
                     /gene="ylqF"
                     /locus_tag="ACHAYF_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8578928.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            25776..26174
                     /locus_tag="ACHAYF_001487"
     CDS             25776..26174
                     /locus_tag="ACHAYF_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8578929.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         7034 a         5589 c         4544 g         9202 t
ORIGIN      
        1 aagcttcttc cattagttgt atttgtacca tatgtcgctg tgtttttgag gaacgaaaaa
       61 caatttcttt tccgtaagac ttatgccgat ttttttctac aaatcgttcc gtttgtatat
      121 aagggatttt ttcgtcagct tgtccatgcc aaaagaacag aggacgtcca gctaactttt
      181 ccggctgcaa tgacaaatca tagctcttaa cccatgaaat caaatcaaaa tagtcttttg
      241 gtacgacata attatattcc gatgcgcgcc ttacgatttc ttttccatat tccaaaggtg
      301 caggggaacc catcaaacac acgcccgcta ctatatgagg atgtttagcc aacaaggcgc
      361 atgttgtcat tccccccatc gaaaccccgc caactgcaag ctgatcatgt agaagacctc
      421 ttttttctaa atgcttgata agtgtgtcaa attcaaacag atttccgtag atactatccc
      481 aaaatgtata ggatggaata gaagaaaccg gctgtttgcg ctccccatga ttcatcgcat
      541 ctggcaggat cacacgaatg ccctgctgcg caatttttct tccttgcgtc aaaactaact
      601 ccttacttgt ctgccatccg tgataataga tgacaacggg aatacgttcc tctcttaatt
      661 ctttcagaac aacttctaac actggaatat gatggacaga acgtttcaat ataattggat
      721 tcaattgtta ctcacctatt ttcattaaat ttcctgatcg ccatagcaac catgctccaa
      781 cagttgtctg gaacaggttc agtgtaccaa ataatataaa tgcaggactg aatggctcgg
      841 gaaataagca cagagaacaa agtcccacca ctcctaatgc tagcgaaagg agtggataaa
      901 tagaaaaatt caaaaaactg gcaacgctac cgaaaatatt accaataaac agtatcaaaa
      961 agaaaaaaac gcttaaaatt aaaccatctg tcagcgacat ctgaaaaaca atgttctcta
     1021 cctcaattgg ctgttgaatc gaatagacac ctaagtatat actgagaagg agacttagca
     1081 cgtggctggc acaaaacaca cgattgaccg ttttcatttt aatcacctaa cctatcttta
     1141 taattctatt ataatcattt tatcattttt ttgtatgtat aaagtttcaa tttttcttca
     1201 cttacgattt tttttgatat tgttgttata taaatgaaag gagttttaca aatgattgat
     1261 ccaaaaatgt tggggatgat cttcctcatt aattttgctt atatcactct gaacacgtta
     1321 cgtttcatgt tgacaatgaa aggctatcga ctgattgctc cacttgtcag tatggtcgag
     1381 atcacgattt atatcgtcgg cttatccctt gttttagatc gtttagataa tccactgaat
     1441 ttatttgtct atgcgttagg ttacgcagta ggtatcagtg tcggaatttt gattgaagac
     1501 aaattggcat taggatacat catggtcaca acgattttgc cagcaaatag cgcagaagat
     1561 aaaagtttgc cgggtatact gagagaacac ggttatggtg tgacacagac ttctgcaatg
     1621 gggctggaag gtgagcggct agttctcgaa atactttctc ctagaaaaag cgaacgtgaa
     1681 ctgtatcgcc tgatcaaaga aatcgaagag cgtgctttta tcatttctta tgagccaaaa
     1741 tatatttcag gtggtttctg gactaaaaaa gtgagaaaac gacaaggaaa ataactcaca
     1801 aacttaaaaa ctgtctaata tctaaattaa attttaaatg ttagacagtt tttatttatt
     1861 attttgatta aaaaacatcg acatatactc atcaatttgt tattatactt tatatactta
     1921 tcaatttaat ttggagatat tatggaaatt caacgtagaa ccactaattt tttacattta
     1981 ggctcttttt ttgcaacttt gacgatctta ttttttctat gttattcata ccttaagacc
     2041 tcacatgcgc cttctagtgc attaggctgg ttgcttttgg atcattctga tatcacagaa
     2101 gccgaagaaa atccgttttt tttgattttg tcttctcttt gcggcttact gttttctgtt
     2161 tatttcggtg cctatttttt tcatcgacat cctggtacgt taaacgaagc ttcaagcgta
     2221 ttgattgcat ttaaaacgtt ttctttttta gcaattcttt tttattcgtt taatcacaat
     2281 acgcttttct tctttatcat gaatggttta gtcgtgattt tatccatttt tacttatttt
     2341 attttttcac tcatttttag ccaattgaaa tgtccgctct tctttcgatt agttcctgtc
     2401 acctgtttta tttacagctt gttttccttt tttgtccttg ctttaatgcc actgaccaac
     2461 ccttcgacga tccaagtcgc aggaaatctg ttggatatgc tcttcatcgt cagtctaggt
     2521 gtatttatgt ggcaaagaaa aaacaaacaa aataaaaagc attttgaagc ctgactatta
     2581 cgacttcata aatgcttttt tagttttaaa cacttcaatc taaaagaata acccaagatt
     2641 ggataaatta ttaatgactc gccattcgtt tcttagtgat tctggattat caaatggact
     2701 tcctaatcta atcagctagt tcttcccata aaatgccata cttgactgaa tcataatatt
     2761 gattatttaa aaatctaact tttcttatca ctacttcctt tgtcattcca catttctcac
     2821 catttttttg cattcctttg tttccagacc atgtagtgaa tctaataggt ggtagatagg
     2881 caaaatactc aaataattct tttaaccata aaggaagaga aaggctcctc ctgcaagagt
     2941 cagtataatt tttgcttact gctctcttag gttttactca ctaaaactgt tccatattct
     3001 ttgatataag aaaatgcatg cggtataaat ttcttgctca gctcagaaaa gatttgtcgt
     3061 ttaagaaagg ccatttcctc cattgtcaat gaaggatcca atgttggtaa ttcaatctca
     3121 aaattgtttt tttcgacaat aggtaattct aatgtgaatg attcttcttc ctttatagta
     3181 ttcgtcacaa gtagagactg atggaaaata ctaggccggc gatttttata tttattctta
     3241 cgtatcctgt caacgataac ttctatttga tcaaaaaagt acgattcatt tacgtctcca
     3301 ataagttcgg ctaattcttt gaatgccttc gttacaggaa gctgattctt ctgtacaaaa
     3361 aagtaattag ccaaagtaag cgggaagtgt tctatagagg aaagtgtgat aaaacggtac
     3421 ttctcatttc ctgcagttgt cctcagcaat tctgtctctc ggctgtttct tactccataa
     3481 aaatacgctt ctttccgttc atcttcgaaa caatacgccc aaataacttc tcccatcgct
     3541 aatttttgtt catcaacaga gagtctcttc aattgttcag ctatggtttc agcagccgaa
     3601 gtggcttgtt gcaagtaatc tttaggatca aatattggaa agttcaaatg atatcttcgt
     3661 tcttccctct tgattaatcc tgcatcgatc aataaatcaa gaaaatggga aaatttattt
     3721 tgcgaaaatt cttttttcaa ttctcttaga atcgtttctt gttctcgatg ttcatataga
     3781 taatcggtca atgaagaaaa aagcggatca tcggtcagtt tttgataaaa gttttcctta
     3841 ttttttgaat aatagttcat atatgttctc ttttctatcc gttttatttt acttaaaagg
     3901 cttttacgta aaaaggagga agaacaagag tcataattga ctgttattct cctccatttc
     3961 ttcggccttt agtatgttct atgtttcttt ttatatcaat cgcttatcca ttcattttga
     4021 atgcttcata ctaccatttg attagctaaa tacagccgtc aattgaggta cgacttgttt
     4081 tttacgtgaa acgacacctt taagcaaagc acggttgttg gtcaaagttg tttggaaagc
     4141 ttcttctact ttttcttttc ctttaccagc aacaagtaat tctgaatcac tgtctaagat
     4201 gtttgtgaca actaatacga acagatcgta gccatttgct ttcatttcat cttccattgc
     4261 tgcttctagc tctgcttgtc gatccatcac ttcattaagg tccactgtat tgacttgagc
     4321 gatacgaacg ttggaactgc ccattgggaa actttttgca tccaaatcaa gaagaaccgc
     4381 agctgattta tcacttagat ttgttcctgc tttcaacatt tcaagtccat aagtgtttag
     4441 atcgacttca gcgattgctg caagttcttt tgcagctgct tcatcttcag gcgtacatgt
     4501 tggtgatttg aacaacaatg tatctgaaat gatagctgag agcatgattc ctgcgatttc
     4561 tttaggaatt gttacttggt tttctttata tagttttaaa ataatcgtac ttgtacatcc
     4621 aactggttct gcacgatagt acaatggatt agctgtttca aagtttgcaa tacgatggtg
     4681 gtctactaca gctaaaatat ttacttcgct gatatctgaa atactttgtt ggaattcatt
     4741 atgatccaca agcatgacat ctgcattttc tgctttagct gactctacta ctcgtggtgc
     4801 agatagctga aagtggtcta atgcgtattg tgtttcttca ttaggtgtac ctagagcaac
     4861 tgcttctgtt tctttaccta attcattttg caagtgtgaa aacgcaatgg ctgccccgat
     4921 tgcatctgta tctggatttt ggtgaccaaa aactaaaatt tttgacatgt cattcgctcc
     4981 tttgtgattt ctcctattta ataatagcaa aaaaacggaa agaagcaata cttatcttct
     5041 ttccgatgcg acctatcgcg ttaaatattt tgtatactct tctgtatgaa gcaatcgttt
     5101 agcattttcg acacgttctt ttgtaggtgg ttcgatccct tctaatggat atttcaaacc
     5161 aagtgtttcc cacttgtatt ttcccatcgt atgataagga agaatctcta ctctgtctac
     5221 atttgacaag gtttgaataa agttgtctag tcggatcaaa tattcatcat aatcacttcg
     5281 ctcaggaact agtacatggc gaatccaaac cggtttgttg atttttgaca aatattgagc
     5341 catttctaaa atattttcat tgtcccaatg cgttagtttt ttatgctctt cattatcgat
     5401 gtgtttgatg tcaaataaca gtaaatctgt atacttcatc aattcttgaa aacgtgaaaa
     5461 gaaaggttct tcatacgtaa aaggttttcc gcatgtatcc aatgtagtat gcatcttccg
     5521 ctcttttgct tttttaaata gttcgatcaa aaaatcgatc tgcaatagag gttctccacc
     5581 actaacagta ataccacctt tatctcccca gtaaggtctg aagcgttctg ctttatctaa
     5641 aagttcatct gctgtgtatt ctttccctcc gccaatattc caagtatctg gattgtgaca
     5701 aaattggcaa cgcatgcggc atccttgcat aaacacgaca aagcgcagtc caggtccgtc
     5761 tactgaaccg aaagtttcga tagaatgaac atagccgatt gttttttctt ccatttaagt
     5821 tcacatcttt tctttgattt cattttaagt acaagttagc tgtcgaagcg aagctttctg
     5881 aacaaaattt ttcgcaattg ccgcttcgtt atgtgtaaaa tttacaggga cggaacatac
     5941 aagtcctgcc cctgtaaatc gtttctttca aataagcata ctgattattc gattttgatg
     6001 cgatggctat tcaacgtcct ttcgacgcta agtcgaaaaa cttttttcac gaacttttta
     6061 cattctgtcg tgtgaagttc tcatcaagac atctaattgt tgttctcttg tcaagtcacg
     6121 gaatttaact gcataaccag atacccggat cgtcaagttc ggatatttca ttggattttc
     6181 cattgcatca cgcaataaat cattactgaa tacattgaca ttcaaatgat aagctccttt
     6241 tgagaagtat ccatctaaga cattacgcaa gttttcgatt cttgtatctt gttcttttcc
     6301 aagacctgaa ggattgattg tttgggtatt tgaaattcca tccaatgcac agccgtaatt
     6361 cattttagct gttgagttca ggctagctaa taacccactt tgttccgcat tgtaacttgg
     6421 atttgctcct ggcgcaagtg gttttccagc ttcacggcca tcaggagtat ttcctgtatt
     6481 tttaccgtaa acaacatttg aagtaatcgt caataatgaa gttgttggtt ttgagttgcg
     6541 gtaagtttta tgacgtttga tttgtcccat gaagtattcc aagatccatg aaactaattc
     6601 atctgcacgg tcatcatcat ttccgtattt agggaagtcg ccatccactt caaaatcaac
     6661 tgctattcct ttttcattac ggattgcttt tacttttgca tatttgatcg ctgacaagct
     6721 gtctgccgca tgagagatcc cagcaatacc tgtagcaaat gtgcgttgca agtcagaatc
     6781 catcaaagcc agctgagctg cttcataaga atatttatca tgcatataat gaatgatgtt
     6841 caacgtattg acatatagtt ctgctaacca atccatcata ttcatgaagt tatgtttcac
     6901 ttcttcaaag tctagtattt ctgatgtgat tggacgatat tctggtccaa cttgggcgcc
     6961 agtcacttca tcgactccgc cattgatggc atataatagt gttttagcca agttagcacg
     7021 agcgccaaag aattgcatgt cttttccgac aactgttcct gatacacagc aagcaatcgc
     7081 acaatcatca gaaccccatt gttcacgaag ctgattatca ttttcgaatt gaatcgaaga
     7141 actttcgata gcgattctgc tagcatatgt tctgaatcct tctggaaggt gttcagaata
     7201 caaaacagtc aagtttggtt ctggtgaagg tcccatattt gttaatgtat gaagcagacg
     7261 gaaatcattc tttgtaacta atgaacgacc atctaatccc atacctgcaa tggataaagt
     7321 agcccagata gggaatcctg agaataattg gttatattca ggtgtacgcg cgaatttgac
     7381 catacgtaat ttcattacta agtggtcgat caattcttgt gcttcttttt ctgtgattac
     7441 tccattatcc aaatcacgtt ggatatagat atcaaggaat gctgagatac gtccgattga
     7501 catcgcagca ccattttgtg atttgattgc tgctaaatat ccgaagtata gccattggac
     7561 agcttctttt gcatttgcag ctggtttaga aatatcatag ccatatttcg cagccatttc
     7621 tttcatttgc gctaatgcac gatattgttc agtgatttct tcacgtaaac gaatcacttc
     7681 ttcactcatt gtatgataac cagtcatatc atgatctttt ttcttttgtt ccatcaaata
     7741 gtcgattcca tacaatgcca cacgacgata gtcaccgatg atacgtccgc gaccgtaagc
     7801 gtctggaagc cctgtaatga ttttgttttt acgagcagca cgcatttcag cagtataagc
     7861 gtcaaataca ccttgattat gtgttttgcg ccagtcagtg aaaatatgtg tcaattcatc
     7921 gtttggtgta tagccattgc tttttagcgc attgttagcc atattgattc cgccgaacgg
     7981 catgaatgct tgtttcaaag gtttgtctgt ctgtagaccg acgatttttt ccaaatcttt
     8041 atccaaatag cctgcatcgt gtgaagtaat agttgatacg atttctgtgt ccatatcata
     8101 gacaccgtta cgctcgaatt ggatatcatt taacttttgc aattcatccc ataatttttc
     8161 tgttgcttct gttgggcctt ccaaaaagct gtcatcaccg cgatattcag tgtagttatt
     8221 ttgaataaag tctctagtgt ctacgccttc tttccatttg gtacctttaa atccattcca
     8281 ttgctccatt gtatggacct cctaaatttc tgttttatgt aacagttttt acgtgattag
     8341 tataacacag caccctatct ttgcaagggt tttcttgttt tttatttaac aatttttatg
     8401 ttttatttac atatatttaa atcaacgttg acataccaac atttaagcat tgtgaacact
     8461 ttcatctttt tttgcaaacg tataaggatt tattaaaaat gaatgaaaaa gagacgccac
     8521 ctgtattaat acagatggcg tctcttcatt aaaatactac aacagttacg actcgatgaa
     8581 aatctcagga gcatcgataa cttcgatgac ttctccacct tttttctcat ccatcgcaaa
     8641 tgatccattc gatgttcgat cacctatagg gaaatcagtt gggtcaatcg ttaactgctg
     8701 ccctttttga ttcatcaagg ttatttcttt tgatcttcct tccgacatat agataatacg
     8761 gtgcggattt tttttcagct cacgcatgac cgtcactccc cgcttcgcac gtgaagtttg
     8821 gctgatttct tgagcaagca tgcgtttaac agctcctcgc tgagtaacga ttacgatcgg
     8881 cgtatcacct tctgctaaca caagcagccc gttgataatc ctatctccat ctttcaaatt
     8941 catagacttc actccagctg cctttgctcc aacaacggga acatctgtta aaggatagcg
     9001 caaacccatt ccttgatgcg ttactaagaa aacatctaag cctggctgat cttctttcag
     9061 atacacagca atcagttcat cttcttccgc ctttagcttc atacagctga gcggacggct
     9121 cttataggtg cgccatggtt cgaactctgt cattcgtgtt tgcttgatca atccattctt
     9181 gctgatgaat acaaatgttt tctctgcttg aattttttga tatgggaaaa cagctaaaat
     9241 cgattcgtct acagacagat tggagattgt ttgagaaatg tgctcaccag catctttcca
     9301 tttcaaatcg ggtagttcat ggacagggcg gtagatcaca tttgctttat ttgtgatcag
     9361 aagaacatgg tccaatgtac tcaattcacc tgagtatagc aagtagtcgc cttctcgcat
     9421 cccgatttct tctggtttgg aagcactata agaacgaagg cttgttcgct tcacatagcc
     9481 ttctcgcgtc acactgacaa ttacgtcttc ttgtgccact aagacttgcg tatcaatttt
     9541 gatttcttcg atttcgtctt cgattgtcgt tagacgagcg gaagaataat tttttttcac
     9601 ttctcgcaat tcttttttca taacagagaa aagctctttg tcatttgaaa ggattttatt
     9661 caactctgtg atttgcgcaa tcaagctttc ggattccttt tgaagctctg tgatatccgt
     9721 gttcgtcaaa cggtataatt gcaatgtgac aattgcttct gcttgttctt ctgtaaattg
     9781 aaagacgtcg actaaattct ttttggcgtc ttttttgtct ttactttcac gaatcgttgc
     9841 gattacttca tccagaatcg ataaagcttt catcaatcct tctacgatat gctgtctttt
     9901 ttgagctttg gctaaatcga attgactacg ttttgttatg acttgctttc gatgctcgat
     9961 ataacttctt aaaatgtctt tcagtccaac ttgatgcgga gtcatatggt cgatagcgac
    10021 catattgaaa ttgtaattga tctgtagctc agtgtttttg aacaaataat tcaaaattcc
    10081 ttgagcattg gcgtcttttt tcaattcgac gacaatttgc agacctgttc tatcactttc
    10141 atctcgtact tcagctattc catcgatttt cttattcaaa cggatctcat ccattttttt
    10201 caccaaggtc gctttattta cttcatatgg gatttctgtg atcacgattt gctgtttatt
    10261 acccttcatt gcctctatct ttgttttcga acgaagaata acttttcctc gtcctgtttc
    10321 ataagctttt ttgatttcat cttttccttg caagatccct cctgttggaa agtcaggtcc
    10381 aggaatatat tccatcaatt tttcaaggct cgcttgtgga tgatcgatca tatagattgt
    10441 gccatcgatc acttcagcta agttatgagt cgggatttct gttgcatatc cagcagatat
    10501 acctgtcgat ccattgacga gaaggtttgg gtatcgagct ggaaggactg ttggttcttt
    10561 ttctgtatca tcaaaattcc acacgagatc gactgtttct ttctcgatat ctgctagcat
    10621 ctctccactt aacttggata atctggcttc agtataacgc atcgcagcag gtggatcacc
    10681 gtccatactc ccgttattcc cgtgcatttc gatcagtact tcccgcaact tccagtcttg
    10741 gctcatgcgg accatcgctt cataaatact gatgtcacca tgaggatgat aattccccat
    10801 gatatttccg acagattttg ctgatttacg gaatcctttg tcatacgtat tgccatcttt
    10861 gttcatggca aataatatcc ggcgttgcac tggcttcaat ccatcacgga tatcaggcag
    10921 tgcacgttct tggataatat atttggaata acgtccgaag cggtcaccca tcacttcttc
    10981 taaagttaat tcttgtactt cttgtcgatt ttccatcagg tgacctccta ttctacttca
    11041 aataactgat tgttttcatt tttatctgct cgttcttcat ctaataaatc attagaaact
    11101 gaaggagaga tttcagtatc ctctttttta tctaagatac tgccatcttc ttcgagcgta
    11161 aattgcacgt gattttcgat ccattttctt cgaggttcta ctttgtcacc catcagtgtg
    11221 gttactcgac gttcagcttg cgcagcatca tcgatacgga cgcggatcaa cgtacgggaa
    11281 gtcggatcca tcgttgtttc ccacaattgt tccgcattca tctctccaag tcctttatat
    11341 cgctgcagca tatatccttt gccgactttt ttaatcattg ctgccaattc gtcatctgtc
    11401 cacgcatatt caatgacttg cttctttcct tgtcctttgg atactttata taaaggcggt
    11461 aacgcgatat atactttccc agcttcaatc agcggtttca tataacgata aaagaaagta
    11521 agcagtaata cttgaatatg ggcgccgtct gtatccgcat cggtcatgat gattactttg
    11581 tcgtaattac agtcttcgat agaaaactca ggaccgactc ctgcaccaat cgtatagatc
    11641 atcgtattga tttcttcatt tttcaagata tcttgcattt tagctttttc ggtattgatg
    11701 acttttcctc gtaaaggtag aattgcttgg aactttctgt ctcttccttg tttagcagat
    11761 ccgccagcag aatccccttc gactaaatac aattcatttt tcttagggtt tcgggattgt
    11821 gcgggtgtca attttcctga aagaagagat tcgccttttt tacgcttctt cccatttcgg
    11881 ctttcttctc tggctttacg tgctgcttct ctcgcttctc tggctttgat tgctttacgg
    11941 accaacatct ggctcatttc gctattttcc tgcaaataaa agcccatttg ttcactgatg
    12001 acgttatcta ctacggtacg agcaactggc gtaccaagtt tttcttttgt ttgcccttca
    12061 aattgcagaa gattttcagg tacacggata gaaagtacag cagctaaccc ttctctgaaa
    12121 tcactacctt ccagattctt gtcccgttct ttcagcaaac caacttttct cgcatattca
    12181 ttataagatt tcgtcatcgc cgctttcata ccagcttcat gggttccccc atcttttgtc
    12241 cgaacgttat tgacaaaaga taaaacattt tctgaataac cgtcattgta ttgataagcg
    12301 acctctactt cgataccttc tttttctcct gaaaaataaa caactggtgt caatgtatct
    12361 ttttcttcat tcagataatc aacaaattct ttgattcctt cttcgtaatg aaaaatttcc
    12421 ttgactggtt cttctccacg aagatcagaa agagagattt taacgccttt tagaagaaag
    12481 gcagattctc ttaaccgttc ggctaaaatt tcatatgaaa atttcgttgt actgaatatt
    12541 gtatcatctg gtaaaaaatg gacagtcgtc ccgtttgctt tttttgtttt tcctatcttt
    12601 ttcaaagtac cgtcaggttt accgccgttt ttaaatttct gctgatactc tacgccatct
    12661 ctaacgatcg ttacaattag ccatttcgat aaggcattta ccacactggc tcctacaccg
    12721 tgcagtccac cagaagtttt gtaccctcct tgaccaaact ttccgccagc atgcagcaca
    12781 gtaaaaatca cttcaactgt tggtattccg gaagcatgca ttcctacagg catcccacgt
    12841 ccgctatctg caacagttat actattgtct tcatggatgg tcacatcaat ctcactgcca
    12901 taacctgata atgcttcgtc aactgcatta tcgacgattt catagactaa atgatgcaag
    12961 cctcggctgt cagtggatcc aatgtacatc cctggtcttt ttctaactgc ctctaatcct
    13021 tccaatactt ggatggacga gtcattgtat tcgttgttta ctttttttgc caaggcaaac
    13081 gctccttcaa taatactttg cggcaaaacc gcaccacgat tatcatacta gaaaaggtcg
    13141 tgaaaaaaaa gagggtagac gagaaatcaa ttttaattat ctcctaaccg ctttttcgac
    13201 attcccctct atcgatcatc tcttctttgt ggtcattttt atttttggtt ttctttcaac
    13261 ttaccaagtt caatcttaat acaacgatcc attacgatat ctgtacgtcc tgcttttctt
    13321 agcatctcag ctgcttcttt gttttcaatc cctaattgtg cccagaacac tttagcatca
    13381 gttttcaaaa aatcacctgc tatttcaggt aaaaattcac tccgtctaaa tacatctacg
    13441 atatcgattg gatcatcgat ttctgtcaaa ttcgcataga ctttctcgcc tagaatttct
    13501 tcaccagcca ataatggatt tactggtatg attttatatc cattgtcttg caatacctgt
    13561 gcaaccatgt aacttgttcg ttctggttta ttgctcaagc ctacgacggc aatatttttt
    13621 gcttcttcca aatagttgaa aatcgtttct tgtgttggat tttttacaga catttctgcc
    13681 accccttgct tttcttttta ttccatctat tatacttgta tctttcattt tttcaattgt
    13741 tcctgttacc tattcatgct aaaataaagg atagttttca acttgaaaga gggtaaaaaa
    13801 tgaaattgat cttattactt attttagcat atcttttagg ttctattcct tccggtgtct
    13861 ggattggcaa attgtttttc aaaaaagata tccgacaaca cgggagcggt aatactggaa
    13921 cgaccaatac atttcgtgta ttaggcaaaa cagccgggat tgttgtcctg cttatggata
    13981 ttttaaaagg tacattggct acttgtttgc cgatgatctt ccacctttct gggattaacc
    14041 ctctttggtt tggggtatgc gccattcttg gccatacatt tccgattttt gctgggttca
    14101 aaggaggaaa agctgttgcg acaagtgcag gaatgctttt aggatttaat cctgtatttt
    14161 ttgtttattc ttgtatcatc tttatcgtta gtttattttg tacaagtatg gtgagtttga
    14221 gcagtatgat cagtgcagta ttgatcactt tatctacgat cgtccttcct tatgtagctc
    14281 ctgtgatttt ggctaagccc aattggttac tgacgatcat tgccttttta gtttcttcct
    14341 ttatctttta tcggcataaa gacaatatca agcgtatcag aaaccatact gaaagtcgaa
    14401 tcccgtttgg tttgaatagc agcaagaaaa aatcctgata ctttttcatt ccaaagagat
    14461 aaaacgggag tgtgacaaat cgatgtcaca ctcccttatt acttacagga tagctttttt
    14521 acacaccttg gtaaacggtg ttccctgcta acttctcggt cacaacctct tacttcactg
    14581 tgatggaata ttttgtcttg aagatttcat gggcacctag ttgattgatc cctacttttt
    14641 ccatcaagtt tccactactg ttcaccgtgt cagcgattcc gcaccacggt tcgatacaaa
    14701 cgaaaggcgc ttcttttgga taaggagacc agatccctac ataaggcata tctttatagc
    14761 ttaaagttac gctatgggaa gatttttcac ttcgaatggc gtacgcattt agaccccttg
    14821 tttcaaaaac caaggcatct tgctcaaaaa gttcacgatt caaagccaga ttcgtatttg
    14881 tctgacctaa cgtcttttga tccatatcga tgaatgggcc tttcaatggt aggcgtaatc
    14941 ttgatttcaa tggagaaaat gccagataga aatcttcaaa tgacaatcct tcttcaagag
    15001 gtacattgaa agcaggatgt ccaccgatag aaaagaacat ttctttttca ccggtatttg
    15061 ccacttgata atgaaccgtg atgccgtctc ctcctaattc atagctgatc actaattcaa
    15121 aatcaaatgg atacttttct aatgtctccg gcgtactcct taagatgaaa ctagccattt
    15181 cttgtccatg ttcaatgact tcaaaatcca tatcccgagc aaatccgtgt tgccccattt
    15241 gatatgtttt cccttgataa acatactgat catcttttaa acgtccaaca aaagggaata
    15301 aaaccggtgc atgtctgccc caatacgctg gatcaccttg ccagatatac tctaaaccat
    15361 gttcttttga tttcagactg atcaattccg caccgtgttc agcgatttcg gcgatcagct
    15421 ggtcattttg aataataaca gtcattgcca ttcctccaac ttcgacttac tttttaaaat
    15481 gggaaggctg gaacaaacat ttctatctgc cccagtcccc ttacttctaa taactgtgtt
    15541 ttgctaactc gtctttaaaa cgggaattca atacttttaa ataagttccc ttcatgccta
    15601 acgaacgaga ttcaatgatt ccagctgatt ccaattttcg taaagcattg acgatcacag
    15661 aacgagtgat gccgatacta tcggcaatat tagaagcagt cagacgacct tcatctccat
    15721 ccaatgcttc aaagatagct tgtacagcct tcaattcgct ataagataat gtgttgattg
    15781 ccatttggac agccgttgca ctccgtacat ctgcttcgat gcttcgagac ttctgataaa
    15841 ggatctgcat accgacaacc gtagctccat attctgctaa gactagatcg tcatctgcaa
    15901 acgattgttc catacgagat aagataatcg tacccaaacg ttcgccagct ccaaagatcg
    15961 gaacgacagt cgtcagacca aatggatatt tctctcttga ctctaccgga aatgcagtaa
    16021 tatcactagt aattgggata ttagcttcag ttttcatcaa ctgtgccact gtatcagtat
    16081 aagattcagg aaattgtttc tcaacaaaca ttgttttgat tcgttcattg ttcacatcgt
    16141 gacgttcatt ataacctagt aatactccgt cacttcctat gatataggtg ttgctgtcaa
    16201 gaataccacc tagtgtgaca gccattttat cgtaaggcaa atctgcttgc atgtcaaaag
    16261 tattcttttg ctgcaaaagt gtgtttatct tgcgggtttt cgttagcaag tctgtcattg
    16321 tcgtcctccg attttttata aaatatagcg acttaaatct tcatttgccg caattgagcc
    16381 aagtttgtca tccacatatg cttctgtgat cgttatttct cccatttgca tatctggtgc
    16441 ttcaaataat agatcttcta gtagtttttc caaaatggta tgcagtctgc gtgcgccgat
    16501 attatctgtg tcacggttga catcgaaagc gatttgagcg atccgttcaa ttgcttcttt
    16561 tgtaaacgta actgacacat tttctgtacc gatcaacgcg acgtattgtt ttactaaagc
    16621 attatttggt tcggtcagaa tcttcacaaa atcttcagct gtcaaatcat ctaattctac
    16681 ccgtatcggg aagcgtcctt gaagttctgg aattagatca cttggtttag acaaatggaa
    16741 agctcctgaa gcaatgaata ggacgtgatc tgtttggatg atcccgtatt ttgtgtttac
    16801 ttgtgaacct tcgacaattg gcaatatgtc tctttggacg ccttctctgg atacttctcc
    16861 agattgctgt gatttagaag tgatcttatc gatttcatcg ataaaaataa ttccactttc
    16921 ttccgctaat ttgattgcct cactatggat atcagcatcc ttgactaatt ttgctgattc
    16981 ttccttgatc aataactcta aagcttcttt gactgttacc gtccgttcca cttttttctt
    17041 cggtgtcaaa gcacctagag tttcatttag gtcgatcccc atttgctcca atccattgtt
    17101 catcattggt gcaggtttct tttggtcttc aacttcaatg gttacttcac gactatccag
    17161 taaacccttt tgtagttgtt ccaagactgt acggcgattg attcgaatat cttctgtcac
    17221 ttcttcttgc ggttcagcgg tattttgctg actagcatta aaaagattca tcatttgttc
    17281 aaattgatta gtggattgtt tctgttcttt tttgatacca ggtaccaaga tttttaccaa
    17341 acgtttttct gcattttttt tagcacttgt gtatacacgg ctgtactgct ctttttctac
    17401 gatttggatc gcattttcta ctaaatcccg aaccattgat tctacatctc ttccgacata
    17461 accgacttca gtaaattttg ttgcttcgac tttgacaaat ggtgcattga cgatttttgc
    17521 caaacgtcgt gcgatttctg ttttaccgac ccctgtaggt ccgatcatca gcatattttt
    17581 tggtgtaata tcttgttgca ttttttcatc taactgcaaa cggcgatagc ggttacgcaa
    17641 agcaacggca acagattttt ttgctgcgtg ctgtcctacg atatattcat ctaattcact
    17701 tacgatttgt cttggtgttt gttttaattc atacatatct actcacctct tacagttctt
    17761 ctacgataat attatgatta gtaaagatac agatatctgc ggcaatattt aacgcatttt
    17821 ctgcaatttc tttcgcactc atgtcgtttt gagcatagtt tttcattgct cgggctgcag
    17881 ccaacgcaaa attccctcct gatccgatag ctaagatacc atcatctggt gtgataactt
    17941 ctcctgtacc tgaaacaaga agcatttctt tatcattcat tacgatcagc atcgcttcaa
    18001 gtttttgcat agattgctgt gtccgccatt cttgagctaa ttcgacagct gcgcgttgta
    18061 gattcccgtt atattcgttt agtttgcctt caaatttttc ttctaaagta aacgcatctg
    18121 caacacttcc ggcgaaacct acaacaactt ctccattgta gatacgtcgt actttctttg
    18181 cagtgccttt catgacaaca gattcaccca ttgttacttg gccatctcca gccatagcaa
    18241 atttcccgtc tttttcgacg gcacagatag tggttgaatg aaatggtgat tcgaccatgt
    18301 ttgtttcctc ctaaatttgt tcttttcctt atttttacgc tcttggatgg aaagaacgat
    18361 agtttttttg taaactttct tttgttacat gggcatagat ctgggtagtc gataaattgg
    18421 catgtcctag caattcttgg actgtccgca aatctgcccc attatttaat aaatgagtag
    18481 caaatgtatg gcgcagcatg tgaggatgga tatcactatc caaactgctt tttttgatca
    18541 tttgattcaa gacatattca attccagtag gcgtgattgg ttttccatga tgattcacaa
    18601 aaacgatatc atgctcttcg tgatattttg tcatcaatac ctggcggcct tccctgaaat
    18661 attcttgcag agcatcttga gcaaaagaac ctagaggagc atatctctct ttgttccctt
    18721 taccatggat caacatgacc tctgaatcaa aatcaatgtc tgataccgac aaactgctac
    18781 actcacttaa acggatgcct gacccgtaaa ggacttccag taaagcgcgg ttcctcaaat
    18841 ccaaaggact atctcctgct acgctgtcaa aaagtgcttg catttcattt tcataaaaaa
    18901 aacgtggcag tctcaaattt ttctttttga gatgaacgta agaaaatgga ttttctttta
    18961 tcacttcttc tttgagaaga tactgataaa acgaacgtag gctggctatc ttgcgactga
    19021 tagaatttct gctatagcta ggacgatgat cagctaatgc actcaaatag acacgaaccg
    19081 taaaatattc cgccttcagc agatcagcgt ctccagattc ctccaaaaaa tgtttgaaat
    19141 ggagaatgtc ttcttcatat gcttctttcg ttttttctga atatcctctt tcgacaatca
    19201 aataacgcaa aaatttttgt tcccacgtgg cttctgtcat tttctcacct tccttttgtc
    19261 ccagagaata atttagcaca gcgtttataa aaagacaaat aaattgtcag aatttaacca
    19321 atatttctaa gtttgttcac aattggcgca attcctcaca ttttgtttga gaaattctta
    19381 agaaaaacgc aagaaaaaga gcgagactat ccgtctcacc ctcttcatgg agtttcctta
    19441 ctcatttggg attgcagttt tttctaattc tgtttttatt gcctcattag cttttaaagc
    19501 tcgttgtgca attgcttcat atcttgcttt tttgtctcga atgcgctctg gcagttcagg
    19561 aaacagacca tagttggcat tcatcggttg gaaatgtttt ccttccgcat gagtaatgta
    19621 gtaagccata cttccaatag ctgtttcttg tggaaactcg actggttcca tttcttttgc
    19681 taagcgtgcg gcgttgattc cagcaagtag tccgctcgct gcactttcaa catagccttc
    19741 tacaccagtc atctgacctg caaagaacag atcttcgcgt ttcgttgatt ggtaagtcgg
    19801 tttcagcaat tctggcgaat tcataaagct gtttcggtgc atcacaccat agcgaacgaa
    19861 ttcagcattt tctaacccag gaatcatccg gaaaacacgt ttttgctcgc cccatttcaa
    19921 gtgagtctga aagcccacga tgttgtataa agaagctgca gcattgtctt gacgcagctg
    19981 gataacagca taaggacgtt tgccagtttt aggatcttcc aatccaactg gtttcatagg
    20041 tccgaaaagc atcgttttga atcctcgctg tgccattact tcaattggca tacatccttc
    20101 aaaatatttt tctttctcaa aagatttaag ttctgccact tcggcattga ttagtgcctc
    20161 atggaaagct ttgaattcct cttctgtcat cggacagttc aagtaagctg cttctccttt
    20221 gtcatagcgg gacttcaaat atactttatc catatcgatc gtagctttgt ctacgattgg
    20281 tgcagctgca tcatagaaat aaaaaccatc tgaaccattg aatgctttga tgctttctgc
    20341 taatggatga gaagtcaaag gtcctgttgc tactacggtg attccttccg gtatttcagt
    20401 aatttcttcg tttatcactg ttactaatgg atgctctttg acttttcggg taattgtttc
    20461 agaaaaagaa tcgcggtcaa cagctaaggc accaccagct ggcactgctg tttcatctgc
    20521 cgaagtgatt actaccgaat tcaatcggcg catttcttct ttcaataacc ctactgcatt
    20581 agccaaactg tttcctctta atgaattgga acatactagt tcagcaaaat tacttgtgtg
    20641 atgcgcctca gtcgatttga ctggacgcat ttcatataaa cggacaggga ctcctgcttg
    20701 tgcgatctgc caagcagctt cacttccggc aagccctgct ccaacaacat ttaccatttt
    20761 caatgaaaga acctcttttc tattattttt ggatgtcttc ttcgtaatct ccatttacac
    20821 agactacttg tttaccgcct ttgactttct tctcgaccag atatccgtca cattttggac
    20881 aatttctgcc cactggttta tcccaagaag tgaattcaca ttctgggaaa cgggaacaac
    20941 catagaatag tctgtttttc ttagattttc gttcaatgac ttgtccttca tgacatactg
    21001 ggcaagtcac accgatttct tttacgattg gtttcgtatt ccgacaatca ggaaaattac
    21061 tgcaagcata aaacttccca tatttcccta attttatcac catcggatgt ccacagacat
    21121 cacaatcgaa tcctgctggt tcatctttga tttgaatttt ttcgattttt tcttccgcat
    21181 tcgtcagttc tttttcaaac ggtttataga aacgatcaac cactttgacc cacgcttccg
    21241 tgccttcttc aattttatcc aaatctcctt ccatttctgc tgtgaaatgg atatcgacga
    21301 tttgagggaa gaattcacag attaatgaat tgacaatttc tcctagttca gtcggctcaa
    21361 atcttttttg tgtcaatttt acatagtagc gtctttggat cgtatcaagt gttggtgcat
    21421 aagtggaagg tcgccctacc ccgttttctt ctagtgcacg aatcaacgta gcctcactaa
    21481 aacgtgctgg tggttgagta aaatgttgtt ttggttcaat atcaacagat tggactacat
    21541 cgccttcttc aagttctgga agaatatttt ctttctcttc tttgccgtca tctctacctt
    21601 ccacatacac ttgcatgaat cctttgaatt tgatttttga accgttcgca atgaagatga
    21661 caccattttg ttctaatgtc actttcattg tatctaaaat agctggtgtc atctgactag
    21721 ccacaaaacg tgaccagatc aatgtataaa gtttcaattg atctttatct aaatattgtt
    21781 tgagttcatc tggtgtacga agcacactcg ttggacgcac agcttcatgg gcatcttgtg
    21841 cgccttgagc atttttgatc tttcggtgat catgtctaga aaattctttt ccatacgtat
    21901 cttcaataaa tgctgcggcc tctgcttttg ctgtatcggc aatccgggta gaatcagtac
    21961 gcatgtatgt aatcaagccg actgttcctt gtttacccaa agtgattcct tcatataatt
    22021 gctgtgctac catcatggtt tttcttgttc gaaagtttaa cttacgggca gcttcttgtt
    22081 gcaggctact tgtagtgaaa ggattcgcag ggtttctttt gcgctctttc ttttctactt
    22141 ttttaacttc gaagtctgtt ccatcaattc tagcagtgac ttcttttaca ctttgtgcat
    22201 ctggtaattt ctttttctta ccgtcgattc cccagaaatt tgctttgaat ttcttgcgag
    22261 cttttttaaa attaccgtcg atgctccaat attcttctgg aacaaattct cggatctctt
    22321 tttcacggtc aataataatt tttaaagcga cagattggac acgtcctgca cttaaccctt
    22381 tcttgacctt acgccagaga ataggactga tcgaataacc aacgattctg tctaaggtac
    22441 gacgagcttg ctgtgcatct actaaatcta catcgatcgt tcttggttcc ttaaaagctg
    22501 ctttgactgc gtcttttgtg atttcattga agaccacccg atttttttct ttcagatcca
    22561 acccaagtag gtacgctaaa tgccaagcaa tcgcttctcc ttctctatcc gggtcacttg
    22621 ccaagtaaac tttttgagct ttttttgctg cggctttcag gcttttgatg acatcgcctt
    22681 ttccgcgtat agaaatataa tggggttcat aattgttttc aaaatcgata cccattttac
    22741 ttttcggtaa atctcttata tggccaacac tggccattac tttatagttt cgtccaagat
    22801 atttctcaat tgtcttggct tttgcaggtg attctacaat cactaaatat ttatacgcca
    22861 ttactgaatg gactcctttc cattgtcgtg ttcctactat taaaagagga ttcatacaaa
    22921 tcgtagctaa tcatatccat tcagaaaacg tttgtcaaga aaagcaaatg aaaagtatgg
    22981 tgttattctt taaattctgc caagatatct tcacccgaaa ccgtgcaaac tgcaccgtct
    23041 tggatcaaat gatgacatcc ggtagaatgt tctgttaaaa tatttcctgg aacagaaaaa
    23101 actgttcttc caagatccaa tgcttgctgt gctgttatca gagagccgct ttttttcttg
    23161 gcttcaaaga cacatgttcc tttagacaaa gctgcgataa tccgatttct cattggaaaa
    23221 caatgctttt tcgggccgga ttgaggagga tattcactca aaagaagatg atcatttgca
    23281 atcacctctt gcaagtcacg attttcagca ggatagactt gctctatccc tgttccaatg
    23341 actgcgattg tccgtccacc atgagagatt gctgcttggt gaccgtatgt atctatccct
    23401 ttcgcgcagc cactgacgat cacatatcca gcttctatca ctggttttag taaataataa
    23461 actgtttttt tggctaaagc agaagtgtgt cttccaccaa caacgcttaa catgttacaa
    23521 gaaagtaaag atagatcacc attataaaac agtacaattg gcggataggt caagtgaagc
    23581 agctgagggg gatatttggg atcaaagcaa gtgataaacg actgtttcga ttggattttg
    23641 ccccagttag cgtgaatttc ctgccaactt ttgcgaaaca gctcccggtg tttggttatt
    23701 ttacctatct taatgatgtc ttcaaatccg atttctttat aaggatactg catcatccag
    23761 ttagcaagtg cccattttcc ttgactactg attccttttg tataagcaag tttcaataaa
    23821 ttttcttcta tttgatacat cataaaaccg cctttcgtct ctctttctaa taagatacgt
    23881 aaaagcggct gttttttatt gtaaatattt ttttacaggt gcaaaacttt tacgatggat
    23941 tggcgtaatt ccttgtcgtt caagtccttc taagtgttcc tttgttccat aacctgcatt
    24001 atgcgagaaa ccatatccag gataaagcac gtcatattct ttcatcaatt ggtctcgata
    24061 tactttagca atgatacttg cagcagcaat cgacacagat cgtgcatccc ctttgattat
    24121 tttttcctgc tgaataggca aatccaaggt catcgcatcg atcaacaaat agcttggtac
    24181 aatggacaat ttttctactg cttggatcat cgctaattta cttgcttcat aaatattcac
    24241 atcatcgatt gtctgctgat caacgatccc gatcccgatt gccagggctt tttcttgaac
    24301 ttgttgataa agaagttctc gctttttctc tgataattgt ttggagtcat tcagccctaa
    24361 tatgatctca tttttcggca agataactgc agctgccacg acaggtccag ctaatggtcc
    24421 tcttcctact tcatcaatgc cggcaatcca ttggtgacct tgtgcgtacc cttgcttttc
    24481 aaagcaaaac atttcttcat atttttcctt aattaatgct tcctttttca atcgtcgttc
    24541 aaaagaagcc agtgcttgct gcacgccttt gcgtggatca tttcttaatg attctagtgt
    24601 aggatcatct attctctgta tgcttttcaa tcgttctttg atactttgga tggattcagt
    24661 catcactttt ttgctccatt tcttcgcaac gatccagtgt gtatcgtcca agttttcctt
    24721 gacgaatatc atgcacgact aattcgctgc cacgatcata gtcttcttta tacccacgtt
    24781 ttgctgtaat atccatcagt aattccggtg ccaatttttc ttcttgctcg attgataaac
    24841 cataacgatc gctgattctt cctgaataat atcgggcaaa aaagtctaat ccgtaaatcg
    24901 ctaaatcatc taaatggaca agctggtctt tgatcgcgcc tgtcagcgct agttttttgc
    24961 cgatttcttc atcttcaaat ttgggccata aaatccctgg tgtgtctaac aattccaact
    25021 cattacctaa acgaagccac tgctgacctt ttgttacgcc aggcttattt cctgtctgtg
    25081 caattttctt gcctactagt cgattcatca acgtagattt cccaacattt ggaataccta
    25141 agaccattgc ccgaatagcg cgaggcttga tcccccgagc agcatctctt gcccgttttt
    25201 ctttcaatgc ttctttagcc gcaggtacta gacctttaat tcctttactt tcttgtgccg
    25261 tgatagctaa tgcaagatgg ccttgtttac gaaaatattc ttgccacagc tttgtctgtt
    25321 gtggatctgc cagatccgct ttattcaata aaacaaggcg aggtttttgc tgaatgatct
    25381 gatctagcat tggattacga gaagataaag gtaatcgtgc gtctaccaat tcaaatacga
    25441 tgtccacata tttcattttt tcagaaactt ctcgtcgggc ttttgccata tgtcccggaa
    25501 accattggat ggtcattgtt ctctccattc ctttcatacc ggaatattcc ccggctttac
    25561 ttgttttttt ctttttcaat ataaattcac ttttttatta taaagaaatt ccatctatta
    25621 gttaagtgaa agtgaaccag tcctcattct atcacatctt cacttttttg atgatgcaaa
    25681 tttttatttt ggtttctata tatgaatcta aatttttgac agcgtttcct aaataagaga
    25741 tgatagacga acaaaacaga aaggagacga aacaaatgaa tagtatggtt tttgttaatt
    25801 ttcctgttgc tgatgtcaat cgttccgttg ctttttatga aaagctgggt tttaaaaaaa
    25861 atgaagaatt ttctactgaa gaagctggtt caatggtatg ggatgacact ttttggataa
    25921 tgcttctctc tcatgatttt tataaaaaat ttataaaaga taaagaaata gctgataata
    25981 aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa tgcggatgaa gtaaaaaaat
    26041 ttgctcaaat tgcaaaagaa aatggtggta ctttccacga agtagatatg ggaatgcctg
    26101 aagaagaaat gttttcatta gaagtccaag atctagacgg aaatacatta gaacccgtat
    26161 ggatgaagat gtaagcagat aactaatctg acgtttttta taacaaaaaa acaaaggcgg
    26221 atgatacgat aagtattctc cgcctctatt ttttttattc gtacaatctc ttttttacag
    26281 tttataaaag cagtcttctc accctttttt taattgttag atgccagtgt atgtaaatca
    26341 ccaatgtgtc atgacacatt ggtgatttt
//
LOCUS       DAYYCQ010000037        26261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000037 DAYYCQ010000000
VERSION     DAYYCQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.037"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..336)
                     /locus_tag="ACHAYF_001488"
     CDS             complement(<1..336)
                     /locus_tag="ACHAYF_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFM8578930.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDINLLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIK"
     gene            complement(427..879)
                     /locus_tag="ACHAYF_001489"
     CDS             complement(427..879)
                     /locus_tag="ACHAYF_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8578931.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(892..1302)
                     /locus_tag="ACHAYF_001490"
     CDS             complement(892..1302)
                     /locus_tag="ACHAYF_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8578932.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1577..1789
                     /locus_tag="ACHAYF_001491"
     CDS             1577..1789
                     /locus_tag="ACHAYF_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8578933.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1792..2004
                     /locus_tag="ACHAYF_001492"
     CDS             1792..2004
                     /locus_tag="ACHAYF_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578934.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2144..2299
                     /locus_tag="ACHAYF_001493"
     CDS             2144..2299
                     /locus_tag="ACHAYF_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578935.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2296..2520)
                     /locus_tag="ACHAYF_001494"
     CDS             complement(2296..2520)
                     /locus_tag="ACHAYF_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8578936.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2598..2786
                     /locus_tag="ACHAYF_001495"
     CDS             2598..2786
                     /locus_tag="ACHAYF_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578937.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2925..3053
                     /locus_tag="ACHAYF_001496"
     CDS             2925..3053
                     /locus_tag="ACHAYF_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578938.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            3112..3447
                     /locus_tag="ACHAYF_001497"
     CDS             3112..3447
                     /locus_tag="ACHAYF_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578939.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3680..4621
                     /locus_tag="ACHAYF_001498"
     CDS             3680..4621
                     /locus_tag="ACHAYF_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8578940.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4623..5513
                     /locus_tag="ACHAYF_001499"
     CDS             4623..5513
                     /locus_tag="ACHAYF_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8578941.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5556..6356
                     /locus_tag="ACHAYF_001500"
     CDS             5556..6356
                     /locus_tag="ACHAYF_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8578942.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6371..7222
                     /locus_tag="ACHAYF_001501"
     CDS             6371..7222
                     /locus_tag="ACHAYF_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8578943.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7423..7884
                     /locus_tag="ACHAYF_001502"
     CDS             7423..7884
                     /locus_tag="ACHAYF_001502"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8578944.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7910..8362
                     /locus_tag="ACHAYF_001503"
     CDS             7910..8362
                     /locus_tag="ACHAYF_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578945.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8468..8662
                     /locus_tag="ACHAYF_001504"
     CDS             8468..8662
                     /locus_tag="ACHAYF_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578946.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8693..9079
                     /locus_tag="ACHAYF_001505"
     CDS             8693..9079
                     /locus_tag="ACHAYF_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578947.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            9076..9279
                     /locus_tag="ACHAYF_001506"
     CDS             9076..9279
                     /locus_tag="ACHAYF_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8578948.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9276..9572
                     /locus_tag="ACHAYF_001507"
     CDS             9276..9572
                     /locus_tag="ACHAYF_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578949.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9648..10064
                     /locus_tag="ACHAYF_001508"
     CDS             9648..10064
                     /locus_tag="ACHAYF_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8578950.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10325..10462
                     /locus_tag="ACHAYF_001509"
     CDS             10325..10462
                     /locus_tag="ACHAYF_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578951.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10872..11102
                     /locus_tag="ACHAYF_001510"
     CDS             10872..11102
                     /locus_tag="ACHAYF_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578952.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11261..12094
                     /gene="terS"
                     /locus_tag="ACHAYF_001511"
     CDS             11261..12094
                     /gene="terS"
                     /locus_tag="ACHAYF_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8578953.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            12087..13502
                     /locus_tag="ACHAYF_001512"
     CDS             12087..13502
                     /locus_tag="ACHAYF_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM8578954.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13514..15043
                     /locus_tag="ACHAYF_001513"
     CDS             13514..15043
                     /locus_tag="ACHAYF_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8578955.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14961..16013
                     /locus_tag="ACHAYF_001514"
     CDS             14961..16013
                     /locus_tag="ACHAYF_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8578956.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            16015..16332
                     /locus_tag="ACHAYF_001515"
     CDS             16015..16332
                     /locus_tag="ACHAYF_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578957.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16459..17082
                     /locus_tag="ACHAYF_001516"
     CDS             16459..17082
                     /locus_tag="ACHAYF_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8578958.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            17097..17987
                     /locus_tag="ACHAYF_001517"
     CDS             17097..17987
                     /locus_tag="ACHAYF_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8578959.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            18010..18225
                     /locus_tag="ACHAYF_001518"
     CDS             18010..18225
                     /locus_tag="ACHAYF_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578960.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18237..18578
                     /locus_tag="ACHAYF_001519"
     CDS             18237..18578
                     /locus_tag="ACHAYF_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578961.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18578..18949
                     /locus_tag="ACHAYF_001520"
     CDS             18578..18949
                     /locus_tag="ACHAYF_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578962.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18942..19337
                     /locus_tag="ACHAYF_001521"
     CDS             18942..19337
                     /locus_tag="ACHAYF_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8578963.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19339..19716
                     /locus_tag="ACHAYF_001522"
     CDS             19339..19716
                     /locus_tag="ACHAYF_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8578964.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19717..20325
                     /locus_tag="ACHAYF_001523"
     CDS             19717..20325
                     /locus_tag="ACHAYF_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8578965.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20325..20666
                     /locus_tag="ACHAYF_001524"
     CDS             20325..20666
                     /locus_tag="ACHAYF_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578966.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20908..23697
                     /locus_tag="ACHAYF_001525"
     CDS             20908..23697
                     /locus_tag="ACHAYF_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8578967.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23687..24427
                     /locus_tag="ACHAYF_001526"
     CDS             23687..24427
                     /locus_tag="ACHAYF_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8578968.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24424..>26261
                     /locus_tag="ACHAYF_001527"
     CDS             24424..>26261
                     /locus_tag="ACHAYF_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8578969.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9530 a         3730 c         5336 g         7665 t
ORIGIN      
        1 ttttatttca gaagatgaag tttcttctat atttgaaacg ataagtgaag aatagacatt
       61 ttttaatgtt tcatcatact tttgatttat ttcatcattt aaatcttctt tctctttttt
      121 taataaatta atatcatttt ttaacatagt aatttgttta tttaaatctg atagtgtttc
      181 ttccttactt aatataaatc tttctctatc atgtaattta tttagttttt catcattttt
      241 ttgttctaat tcataagctt ctttttttat tgtgttagct ttcgacagtt tagtgattcc
      301 tataattaat aaaataagtc caatcaataa taacattcaa aactcctcat ttctttgata
      361 taattattgt agagtttact cagaaatgag gaaagagtcc atgtgaaagc atgggctttt
      421 ttatttttaa tgttgtaata ctaaatgttc aagattatga ggaagcccta aatattcaat
      481 aatttggaac tgactcatat cagataaatc ttgttcatct attgttctag ttaacatttc
      541 aattgcaaac atattagctt cagcttctat accataaatc atacaactaa ggttgtttgc
      601 tctaaaagct gctgtgcaaa tacctttatg gagtctaacg tgggctattt catgccataa
      661 tacaaatctt ttcatagaag gtgataggtt ttgattaatt aatatcacat aacatctttt
      721 attgtgtatc gtttgtccta atgttttatt ccctaaatca gtttcaatta aatctatatc
      781 agaatatttg attattttat atggattaat cgcatcagat ccaaactcta caaataattt
      841 ttctatttct tctaatatcc agtatttgtt taaatgcata ccaaagccca cctattgacg
      901 atatttttta ggagtgaatt ttttctttgc tttaactttg gttaaggcaa gtgcaagttg
      961 aatggcatcg tataattttt ccttatcttc atcgtctaaa ggttcaccat aataatttgt
     1021 ttcagcatct gaattcaagc cttcaagtaa tttttcagct tgtaaagcaa tatcttgttt
     1081 atctttttct gttaaatcat aataatggcg tttatcagta cgtcctaaaa gataatcagt
     1141 tgatacatta aagtaatctg caactttttg tattttatct ccagatggag tacttttatt
     1201 ccatctagac atgcttccat ttccgaaatt taactttctt tcaagttctg ccaacgtcat
     1261 tgaacgttct tttaataaga tttttatacg gtctaatagc ataggatatg cattcctttc
     1321 aaaataccga caaaaaaagt atttttctgc aaattatcta tttacaaata gagaatttgc
     1381 agttatacta gttttgtaag caagatattt acggcataca aagactaaaa gcaataaact
     1441 aatagtctcc ccagacatca gtaagctcgc tttttgtgcg ctgtattttg tatgcttaca
     1501 taatagcata tttgcagttt ttgtcaaact tattagacaa aaatattaga aaatattcga
     1561 taagggggta tgaagcatga ctttaaaaca gcaagtatta attgctttag taaagaaagg
     1621 ttggagtcaa agagagttag ctcgacgaat gggtatatca attacttatt tacgagatat
     1681 cttcatcgaa aaaagaaaac ctaaggaacg tttaaaacaa atcgaagaac ttttggatat
     1741 caagttagaa gtagattcta atgatcagcc atcagaacag gaggcgtaaa aatggtacaa
     1801 cgtttgagca aagcggagtt taaagagaaa tataagtatt ctgaaagtac ttatcaaaga
     1861 agaatgaagg aatttaaaaa atcacgtttt agtgaggggt ataacgcagt tacttccaaa
     1921 gaaataacaa ttgatgtcga tttatatgaa cagtttagaa catggcgttc taaaaatcga
     1981 tttagagtta gagctgtttg ttaatagcta aaaaatctaa attcaccact tctgaaaaga
     2041 ggagttgctg ctctcctttc aagtttgcca aagtccacgt tttttcattt tggcaactcc
     2101 tcttcttgga ggtggtgacg gcgagagacg aaggagggat gacatgaagg aagaatcaaa
     2161 aatagggatt ttgaatgaga acaattacac aagattaatt attgaaacag aagaagggaa
     2221 aaaagtagca gaagttacat tgaccgatgc aactcctgct gacggttatc gtattcgatt
     2281 aactcctaaa tatgattaac cttttggtgg gtgtggatcg tttccgtgac tatcacggct
     2341 ttgaattttt ccatctctgc catgaataaa tagctcagat ccttgatttt tagaaatctc
     2401 tctagcgatt tttactgctt ctgattttgt attagtatgt gcagtcgctt tagaatttcc
     2461 agcacctttg acattccaac caccattgga attaggtaca acatgttgat tttttcccat
     2521 agttttcacc tccatttcta tagaaatatt ttatcaaata gaaatggaga aattcaataa
     2581 acgaaaggag aagtgatatg gcatatacct tgcaacaaga acatcaaatt ctcggtttga
     2641 ttaaacaacg caggaaacaa ttacaagatg accgtgcagc gcttagaaaa gccgatgagc
     2701 tatcagatag gcaagctgaa ctaattgctt ctgaacttga ggatttgaga atgctagaaa
     2761 taaaaaatag ggagattaga ttatgaagaa gacagacaca ctttttatag gattcatttt
     2821 ggggttatta gtgattgtag cgcaccaaag tattatcggg ggaagcttgt tcgcagcatt
     2881 gatggtttta atcaatctgc ttgattcaaa agaaaggagc aactatggca cgagaagaag
     2941 cgctaaaaat cggtaaagtg attgctgata atcggtgggg aaatagccgc cctattattt
     3001 taagcaagca acatatcgaa aagcaaaaag catggcaaca aataaaaaag tgactctgcc
     3061 agccagcaaa gagtcacata caaatttata aaactaggag aattatatca catgaataag
     3121 aaaattgaaa atcttatcga agaattgaaa cgtgaatgcc aaaagcaagg tgtttctatt
     3181 atttgtacag cccaaaaaga aggggaactt aaaagtctcg tttatggtga gacaacagaa
     3241 attttacttt gtcttgctat gcaagaagaa catttagatg aaaatctccc attgtcagca
     3301 catattatgc gtagaattgc tgtggatgct tacgaacaag cgaaaaatga agaagaaaat
     3361 caaccttcaa atcatacttt tgttattaat aacaaggaag atttagctga tgtgatgact
     3421 cgtattctca agggggaatt tcaatgatga atgaaaaaac aattgatcta ctcattattt
     3481 tatttttcac gttagtgatt gtagcgaaaa gttatgtaat ctttggcttg ttattcgcaa
     3541 ttaccatgct tgtgctttgc gtgttagcaa gcagaaaaga ggttaaacat gaacgagtta
     3601 gcaaatcttg acaactattt aactgatcct gattatgcta aaccaccata tgaagcacca
     3661 attgatgagg aggatgaaga tgagtaaatc taccttagaa atgagccatc aagaatggct
     3721 tgaagaccgt aagaaaggca tcggaggttc ggatgttgga acaattttag gattgaataa
     3781 atggaaatct ccttatcaac tatggctaga aaaaacagga caagtagtac ttgaagaatc
     3841 aggaagtgag cccgcttatt ggggtaatgt tttagaagaa gtggttgcta aagagtttca
     3901 ggaacgtaca ggcaaaaaag tacgcagaag aaaccaagta tttgaacatc cattacatcc
     3961 atttttaaga gcaaatattg atcgggaagt agtgggagaa aatgccattc tggaatgcaa
     4021 aacagctaat caatttctcg gtaaagaatg ggaaggcgaa gaggtaccgc ttagctatct
     4081 ctgtcaagtt caacattaca tgaacgttct aaacaaagac tattgttaca tcgctgtctt
     4141 aatcggtggt caaaaattta tctggaagcg gattgaacga gatcaagagc tgatcgatac
     4201 aatcactgaa caattagtag agttttggga aacaaacgtt cttggaggta tcgagccaat
     4261 tattgatggt agtcaagcga cagctgattt cttgaaagag aagtatgcag atgttgaaga
     4321 cgttcaaacg acgttaccaa ttcatttcga tgaactagtt gaacagaaaa acgaactcaa
     4381 acggactaag aaagaaattg aatcagctat ccgtcaagta gacaacgaga ttatcagcga
     4441 gctaggtaaa cgtgaagcca gtatcggtat cactcaaaaa aacatcatca gttggaaatt
     4501 tgtccgtacg agacgtatga actcgaagaa actagcagag aaatatccag atgtcgcaaa
     4561 tgatgaagag atttataacg ttactgaatc aagaaggcta accgaaaggg agatcaaata
     4621 atatggcaac aaatgaatcg ttaaaaaatc aattggcagc aaagccacag aaacaagttg
     4681 caccaggtca attaggactt aaagctttga tgaatacacc aacaatgaga aagaagttcg
     4741 aagaggtgct tcatgacaat gctaatgctt ttatgtcgaa tgttatgact cttgtatcta
     4801 atgacagtta tcttgcagat agtgaaccga tgtctatcat gagtggtgcg ttaactgctg
     4861 caacattaaa tcttgggcta gataagaatt taggttatgc atatttagtt ccattcaata
     4921 gtaaaaacaa gcaaacagga aaatgggaaa agaaagctca attcatgctt ggctataaag
     4981 gatatatcca attagcccaa cgatcaggta aatacaaagc attaaatgtg atcgaagttt
     5041 acgaaggaga actaaaaagc tggaaccgac tgacagaaga gtttgagttt gatccaaatg
     5101 gtagaacgtc tgatgaagtc attggatatg ttggctattt cgagttactg aatggattca
     5161 aaaaaactgt ctattggacc aaacaagaaa tcgaagctca tcggattgct aacaataaag
     5221 atcgcgataa gacaaagtta agtggtgtgt gggcatctga ttacaatgca atggcacgaa
     5281 aaactgtttt gagaaatctt ctttctaaat ggggaatctt gtccatcgaa atgcaagaag
     5341 ccaccacatc ggatgagaga gtccaaaggg ttcaagaaga cggcagcatt attgctgaaa
     5401 cagaagttga agaagatatt cctgaaagaa aagaagcaga ggttatatct gaagaaaatg
     5461 aagatgtaca aactggatta tttgatgcat ctaatccgcc gttaaacaaa taatgaggga
     5521 gctttctccc ttgcttttct agaggagaaa tacgaatgaa cacaggatat gtcaaattac
     5581 atcgaaaagt gatgaattca ttcgtttgga ccaatcctta tatgtataaa ttatggaact
     5641 tgtgtttgat gaaagcgagt catgagaatc gcaagatact ttttaatgga aaagagatac
     5701 aagtgaacag cggagaattc gtcacagggc gcgatgctat cacatctgag atgaacgaag
     5761 gcgtgaaacg tgaacatcaa gtgaacagcg gttctgtatg gagatggcta aaacaatttg
     5821 aaaaaaacgg aatgttgaac atcaaatcaa ctacgaaata cagcgttata tcaataaaaa
     5881 actggtcttt gtaccaaagc agtgaacaac aaatgaacat caaacgcaca acaagtgaac
     5941 aacaagtgca cacaaacaag aatgaaaaga attataagaa tgaaaagaat aataaaacat
     6001 catcgtcgca accacgcaaa aagcgtgttt acgacaccga ctcagtttac tacattctcg
     6061 cggaggagtt attcaaacag atttgtcaga atcaggaaat caaaaagccg aatctgcaaa
     6121 gttgggcaga taacattcgg aaaatgatcg agatcgacaa gcggaccgaa aaccaggtac
     6181 ggggaatgat tgaatggagt cagcacaatg tgttttgggc atcgaatatt ttatctgcta
     6241 aaaaattgcg agaaaaatac gacacaatgg cagcgcaagc caatcgtgat tataaaacaa
     6301 aacaaactaa aacgcttgaa tacgagaaat ttagcacaga tgagttgcct atttgagagg
     6361 aggcgtaggc atggaaactg ttggggaaat catggaaaag ctgatacaga aagtgcttgt
     6421 ccaacgtggc gaatgtcctg aatgcggaca gcctttgtat ggatggcgta cgaagaaccc
     6481 tgatggctca gaacgttgta agccaacctg tatgcaatgt gggtataaag cattgcgagt
     6541 tcaggaagac ttacaaacag aacgaattta caacgagagc ctgaaagcaa gagcaatcaa
     6601 ttttttcaaa ggtggttctg ttgtgcctaa tcaagcgttg tttgattgca cattgcagaa
     6661 ttatcaaatt gtcgatcaag aaacaagaca agcggttgaa gtaaccaaac gctttgttaa
     6721 ttcagtcttg ttaggaaatc caagtcacct tgttttaact ggaaaacaag gaacaggtaa
     6781 aagtcaccta gcgatggcag cggcttggga agtcttgaag cgatcaaact acgataaaaa
     6841 aatcttattt atcgggttac aggaaatgct ggatcaaatc aaattttcgt acaacaatcc
     6901 tgaactcaga aaaacgattg agggatcgtt gattgcagat atcaaaacag cggatttagt
     6961 catcattgat gatatcggtt cagaactagg aaaagatgca tcagatagtc gagcgtttgg
     7021 cataaacacg ctaaattcgt tcttggatgc acgacagaac ctagcaacga ttatcacaac
     7081 gaacttgctt ggtgaagaac tgaaaaaagc ttacggtacg agaacaatat caagaatgtt
     7141 tgtcaactct gatggattta cgatggtatt ttctcaaaca gcagacaagc gcataaaacc
     7201 agtcaaagga gcgatcgcat gaatttgact aaacaagaaa aaagaatctg tcgtcagata
     7261 atgttgcaaa ttataggtga tcgtgacatg gacgaattat ctcaacaaga gagtcgaatg
     7321 gttcggaaga taagtaagct aattatttat cgggatacag aaagatttct cactgtttta
     7381 ggtaacgtct acgagaatcc agagttattg gaggtagagt aattgccaaa aatattagat
     7441 gcatgttgcg gtagtagatt gttctggttt gataagaaca attctgacgt aacatttatg
     7501 gattgtcgtc agcagtatga agaattatca acaggtcatg tgatcaatgt tgatccagat
     7561 gtggtcgcag actttcgcga tatgcctttt gaagatgatg tgttcgatat ggttgtattt
     7621 gacccgccac acttaagaca tgtcggcgaa aactcatggc ttgccaaaaa atatggaaaa
     7681 ttagacgaac tttggccaga agacatcagg caaggttttg cagagtgtat gcgagtttta
     7741 cgaccttcag gaacattaat ttttaagtgg aacgaggaac agataccact ttctgatgtg
     7801 ttagaggcta ttggagaaca gccattgttt ggtaataaac gaagcaaaac acattggctt
     7861 gtgtttatga aaaaattcag ctaacggagg aaacaaagga ggaaaagtaa tgaagattgt
     7921 attaaataaa tgttttggtg gctttgggtt gagccatgaa gcaaaaatgg aaatttttaa
     7981 aagaaaaaat attgaagtat tcccatacat caataacttt agctatgact cagatgatga
     8041 atacacgaga tacacaggcc aaaagctagg ttcgatggat tttatctact atttcaaaaa
     8101 agacccgaaa atcgacaaag ttacaggaat ttatagcgaa attgaccgac tgtacggaat
     8161 tgctgatgat tccagttttt caagtgattc aaacagaggt gataaggacc tcgttgctgt
     8221 tgtcgaaaaa ctaggaagtg aggccagtgg accatatgca agtcttaaag tcgttgatat
     8281 acccgatggg gctgagtggg aaataagtga ttatgacggc gttgaaactg cacattatgg
     8341 ttttcaaaca ggtagtattt agtccactaa tgacaactaa agaggaggca gaaggatgag
     8401 tgaagatgaa tactttgaac agtagtctat taacgacaga gatttttaac tggaaagggg
     8461 tgtagtcatg gacgcatcgg aagtagtaaa taaaattaat agtctcaaag aggttttcgg
     8521 agacgtcgaa gtagtgataa acgcacaagg ctatgacaac ggatttttta agcagattac
     8581 cgacattagc attcagcaag gaatggaaga cgaagatgga aactttatcg atgaagtagc
     8641 cattttagtg acttgcgagt aagtaattga cagctaaaga ggaggcagaa ggatgaaaaa
     8701 attgcaccaa ctcatatctg agaaagaaag cgaactccaa aatttagaag atagtttggg
     8761 attggggttt ccaattgttg agcaagctaa aatgactcaa atcagtcatc tacgacttga
     8821 attggaagat ttaagacaaa tagaaaaatc aattcaactc aacgacaacc agaaaatcgt
     8881 gttggagtgg ttgaaaagcg agacaatttt aactagagaa gcaccaatat tatctgttaa
     8941 tgctttttct gataaaaatt tactaggaaa attacctgat aaagtacgca aagcttataa
     9001 actattggct tgtaaacaag aatatgaagt cctatcagca ttcgctcaat ggggattaga
     9061 acaggaggaa gcggaatgaa caacagacat cgcagaataa caaaactaag aaaacaggaa
     9121 ctgaatgtac taaagacaaa gtttgaaaaa gaatatggaa tttcagcaga agaaacatat
     9181 aaagtggcaa gtcagtttgt tgctgatgcg agtaatgcta ttcgtaagtt tgggatttcg
     9241 atattaaatg atgatcgtaa atgggaggaa atgagatgaa aataaaagac ggattttacg
     9301 ctagtagtca tggtatcggc ggtttaatgc tagacatgcc gataaagaac cctaaaacac
     9361 gtcagaaatc aaaattcaaa atcggtgaca tggtccgatg taaagcagaa gagttcatct
     9421 atccgtttcg tggatatgta gagcatgtct ataatcattc agcaatcatt cacattgaaa
     9481 acacgatgga atgcgataag tggttagcga aaagcaaaga taatttagca gtggctcgtt
     9541 tggtggatat ggaactaatc aatgacaaat aaaaaagccg gatcgctccg actgattcaa
     9601 taaatccaac acatttatta tatcacataa aggagcggtt tgacttgatg caattgttac
     9661 gagaggtaga tttcaaacag acaagatgta atgcgagaga tgtgctgaag aactttcggc
     9721 gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgccgatt attacggata
     9781 tgccgaaggc accgaagcac ggcaataagg cagaagacgc gatcattcag atgatggata
     9841 tagaagcgga gagagacgca atactagcgg ctttgatggc tcttagtctg attagccgtc
     9901 agatactcta ctacagcttc tgtgacgtaa acaagcactc taattatgaa atagggcaat
     9961 tgatacgagg atacggagag aagaatgtag agaagttgaa atccatcgca ttaatcgaat
    10021 ttgcagaagc atacaaaaaa ggcgtgttag ttcagtatcg ttgattttgt agggtttttg
    10081 tagggatagt gtagggtttt tgagcggttt aacgtgatat tatggtagtg tcgaaagatt
    10141 agtgataggt ctgagacaaa ataaaatgta agggaggaaa tctccctcat cgttttaact
    10201 taagcttcga tagacagcaa cggaaatatt aagaataagg atgtgaattt caactccttc
    10261 taaattgttc ttattatcta tcatccgttg ttgtctatta atttatgcat cggaggaaaa
    10321 acgaatggat aaagaaatca aagtaactgt caaattagat ttgactgaac taaaagaact
    10381 gctcaacaag gctagtgacc aagtcgaaca gttacaagaa actttagatg aaattgctaa
    10441 ttttaaaatc caagtttctt agcagtatat tttttagcag ctgcactcat aaaatcatac
    10501 caagtatcag aaaaatataa cttaactaaa taattaaaaa aggtgggtaa atagaaatga
    10561 cggcgttaat aatttcaatt tttgcgctct gtcttaatgt ctatatgatt ggatttaaaa
    10621 atggacaaaa taaaaaatag tagcagccga gaataatttt atagtgtcac tgtggcggaa
    10681 atagaagacg caaaggtgga ggtaggaagc gtatggcgca ctatcgggac cttgtacaaa
    10741 actagaaagg actagtacgt gtgtggtgcg agccctaggg aggaacagga taaccgcctg
    10801 tgtgtaggtt gctattacat attagatcac tctttgagtg gtctttttat ttacataaag
    10861 gaggctacat aatgagaaac tactggtata tatcgctaac taatgaatat cctcgaacca
    10921 ttgatgattg ttcagtgcgt gttgtgcgtt ctgtccaaat caaaaagaag tactccattg
    10981 ttgaaatgac cagagaagct acgccaaaag agattgataa atgcaaactt ctttattgcg
    11041 gtcatggttt ctatgatgaa ccaaacattc aaaataatat taacaagaat ttgagggatt
    11101 agaatgcaag aaatggctta aaaatctcca atcaggacaa atcaagttag acaaagtttc
    11161 cgatttaaag atattgattg aagctgattt aatgctaaaa gatattgaaa attagaaaac
    11221 aaaactcaac ctaagaagat tgcgaggtgg tgtgtattga atggcaagac aacgtgatcc
    11281 aagacgtgat gaagccaaaa gaatttggtt agaatccaac ggagaaaagc agttaaagga
    11341 aattgcatct gaattaaatg tttcagattc tcaggttaga aaatggaaat cgcaagacaa
    11401 atggagcgct gaattgaaaa gtaacgttac caatggcaaa agtaacgtta ctaatcaagg
    11461 tggcgctcct attggtaatc aaaatgctaa aggtaacaaa ggaaacagca gagcatcacc
    11521 gccaaagaga aataagaatg ctcttaaaac tggtgagtat gaaacaatat tttttgacac
    11581 gttaaccgat aatgagaagg acatctattc tagtttgaat gatgatcctt cttttgcttt
    11641 gtccgaagaa atacgacttc ttaaaataag acaacttcga atgatgaaaa gaatccaaca
    11701 agctgaagct ggactaaatg atgaagaagt cgaacgattg cagcagctaa gaaagattaa
    11761 aactccgatt gaaaaaaatg gtaaaaagct agaaatcaag cgtgaggtta tgcaagatgt
    11821 gcagattagc agaaaaaaac atcgcaaaat tgatgatatt ctttcaattg aagattcatt
    11881 gactcggatt agcaaccagt tagctaaagc catcaagcaa atgaatgaac tttatatgaa
    11941 tgaatacaga actgatttaa ttaaagctca gactgataag atccaagctg agacaaatga
    12001 aattggcgga aataattcag gtgaagaaat agaagaatgg aaacaggcag ttttaaatgc
    12061 cgcaaacaaa cgggcggtga aagaaaatga ataatgaatt tattcctttt gctgatattg
    12121 gttctgccat tgattattac tatgataaac cagtagcttt ttgccaagat attttgcatt
    12181 tgaatcctga tgaatggcaa gaaaatgttt taaatgattt agctgaattt tcaaaggttt
    12241 ctgttcgttc tggtcaagga gttggaaaaa cagcattaga agcaggagca atactttggt
    12301 tcttaacgtg tcgaccctac gctaaagtaa tagcaacagc tccgacaatg aagcaacttt
    12361 acgatgtact ttgggcagag gtagctaaat ggttaaatga tagcttgatc aaaaacttac
    12421 tgaagtggac aaagaccaaa atttatatgg ttggtgattc agagcgttgg tttgctacgg
    12481 ctagaacagc gactaaacca gaaaatatgc aaggttttca cgaggaccat atgttgattg
    12541 tggtagatga agcttctggt gtgtctgatc caattatgga agctattctt ggtacgctat
    12601 caggttttga taataagctg ttgatgtgtg gaaaccctaa taatattgaa ggtgtctttt
    12661 acgattccca caattcagac cgtgataaat acagagttca taaagtatca agctatgata
    12721 gtaaacgtac aaacaaagac aatatagaaa tgattcttaa aaaatatgga aaagaaagtg
    12781 atgttgctcg tgtccgtatt tttggagaat ttcccaaagg tgcgttggat tcatttatca
    12841 gtcttgaaac ggttgaattg gctacagaaa aacaaattag tgattcttta gtcaataaaa
    12901 caacggttgc tcatattggt gttgacgtag ctcgatatgg tgatgattct acgattctct
    12961 ttcctagaat tgctaccagg gcattggagt atgagaagta ttcaaaacgt agcactatgg
    13021 aaacaacagg atatgtcatc aacatggcca agaatctaat gagtcaatat ccgagtattg
    13081 ataaagtgat gattaaagtc gatgacactg gtgtcggagg tggtgtaacc gaccgcctag
    13141 aagaacttat agaagacaag cattatcctt ttgaggtgtt tggagtgaat aacggttcaa
    13201 catcagaaga cgatttttac gataatttag gtactcaact atggggaaac atcaaggaaa
    13261 tgttagaaga aaatatgaca gcaaatctta acggagaaca gcctgttatt gaattgcctt
    13321 ctgatagttc gttaatcaaa gaattaagta ctcgcaaatt caaaatgaca agtagaagtc
    13381 gtatacgttt agaaagtaaa gatgatatga aaaagcgaaa tattggtagt cccgatattg
    13441 ctgacgcact ggctttagcg ttttatgagc caccaagtca ctatcaattt attcaatttt
    13501 aggaggtgag tttttgatta gtacagtatt atctttagag agatataaga agctacgtgt
    13561 taaatatgca acacaaattg aagatggaat gtttgatccg aatggtttta tagaatatat
    13621 gaaaccattt tttgctgatc gcgaaagaaa atatctagct tatactagcg aaaagaatga
    13681 aatagacaat agaccaaagc ctaacactga tattgtaaaa gttaataata aacttcatgc
    13741 tggtatgtat tcaattgtcg tagaccaagc agtcaatcat tttactggta tacctatcaa
    13801 atgggattac gatgtatcgg aacaaaaaag aacactcatt caaagattaa aagataaatt
    13861 cttaaaaaat gatatagaac ttccaacagt tccagaagct tttaacaaat taacaagcaa
    13921 tcttgattcc atgagattcg caatgcttga ttctgaaact gcaacttttc aaggagcttg
    13981 tggagtagct ttccgattgt tagagcctgt ggaagaagat gatggttgga agttaagagc
    14041 aagtaatatt gaaccttgga gagcagaaag atatggaaat gccggaatct atatcaaaga
    14101 aaagtatgac tcttaccaga aaaaattttt tcaagaaatg aaagttatga caagaaataa
    14161 aattctgaca tatgcttgct atggtgattt gaattttgtt acaagcggaa cgttcaaaaa
    14221 aattgatgag accgataacc ctttggggac gattaccttg gcagaattta aaaataatac
    14281 gaatcgttat tgtgattttg aagtagctga ggaaattgga gatgcaattg atcgtgcttt
    14341 gtctgatcaa caaaacgaaa tcgaacaatt taagcttgcg tacatgcttg ttactggcac
    14401 aacaatgagc aaaggcactg ctaaagaaat gatgaatcag ctagggatta tcaacttaaa
    14461 agaccctact gcaaaagctg aatatgtcac aaaaaaatta gcaaaagatt tcaatgaata
    14521 tcatatggat ctattgaaaa aacagtttta caccatttgt aaagcaatcg attttaacga
    14581 tgaggtgttt aaatcaaaca gttctggaga agctcgcaag tggcaaatca ttagcctaga
    14641 agcgaaaaca aatactaaag agcaatattt tagagaagga ttgaaagaat gcgcagaaac
    14701 aattgctgct tttcttaagt ttcatgacaa agtagaaatt gagcctgaaa aaattatttt
    14761 tactttctct agatctttgc caactgattt aagttattta gctgaagctt tacctaaact
    14821 tgcaccatac gtatccaaac gaactattca gagccaaatt ccatttgtaa cagatgttga
    14881 ttatgaaaat gaaatgatgg aattggaaag tggaagtgct tatccagata gtgaatacaa
    14941 ttttggcgga ggtggcaata gtgacgatag aaacgaagta ttggaccaaa cgtcgagaac
    15001 tggaggatca agcaaggctc aaacaagaaa atcagacact taaaaaatta actagtgtat
    15061 ttcctgaagc actgaaagag atacaagcaa aactattatc acaagctgac ttacacaata
    15121 tcacttatcc agaaatgatg gagttttaca gtacaagtaa tcagaaaaaa tatcgtgaat
    15181 atgtggaaaa aaattataag tcattaaaaa tgtatgatgc aaaatacaaa gagtttatcg
    15241 atgaattttt tccaccattt gactatgcaa aagtcaatcg cttattacaa atacgatcag
    15301 atgtatttaa aattcttgca gaatatgcga tggatgcaga tgtgaatcaa tatttttctg
    15361 atcgcttaga ggaaattctt caaagaacat attcttctaa tgctaatgtt tttgttcaac
    15421 ttttaaacgt tgatatacca aattatttac cagaaaatga aattaaacat tatttgaact
    15481 atccatggtc aggaaagaca ttttcaagaa gactttgggg aaatatttca tcgcttgaac
    15541 agaaactctc taatgctatt gtaaaaagtg ttgctagtgg agaaggcgtt atacacgcat
    15601 taaacaccat gagattagac tcagaaattt gtgacatgtt taagttagaa gaatcaaagt
    15661 ataacaaagc gatagaaaat ctcgttcgaa cggagtatgc aaaatttgca caagatggta
    15721 ttgaaaaatc atatttagaa acaggtattg aggaatacaa cgtattgact gcaaaagatg
    15781 agagagtttg ccggatttgt ggaggaaagg caagtaagaa tccctataaa ctgaaagatg
    15841 ctgtcatagg tgaaaatcga gcacctttcc atagtcgttg tagatgtacg gatgttccta
    15901 atttaccaaa attaggaaag gatattgatg aagaatatga ccgtttattt ggcgatttat
    15961 tagatgagtt tgcacatgat tcttttggaa ttaatttgaa acggaggaag tagaatgaaa
    16021 gatttttttg aagcagtact aacaattaat gtaaatgctg atattgcaga agcctacaaa
    16081 acagctattg agtctgagaa ccatcctaat ggcttgagag accattggaa tggcaattat
    16141 gcctacgtgg ttattggcga tcaaactgtt aattatcaag ataatactcc agttgataag
    16201 aataccgtta atttaacgat tcaattatta tctcattcat taccaaattt aaaagaaaca
    16261 gttgattggt atgaaaagat gggatgtatt gttgttagaa ctgactacaa agaaggaaag
    16321 tctagtaatt aggctttttt attttgtccg aaatgacgtt aaactagcgc aatactgggc
    16381 ttggttgaat ggtggggcgc aactattaaa actcaaagca atgcggggcg tgaaaacgaa
    16441 tcgtggggcg aaaggagaat gagcatgaaa cacaaatcat taatgccaat gaatttgcaa
    16501 tactttgccg aaggtgacga tcaaaagttt tcttttgatg acttcaaatc ttttgtggaa
    16561 tccaatgaag aagctcaaaa atttattcag tcacaatctc aaagtgttgc cgacaaacaa
    16621 ttggaagctt ggaaacaaaa taacttagat aaaattaaac aggataccat caaggaatat
    16681 gaggaatcta agaaaaacaa gtcacctgag caaattcaac tggaaaaact acaagctgaa
    16741 tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata aagcatttgt tgcagaacaa
    16801 attgctggat tagaactaga tggagagtta aaagagtcta tttctcaatt tatgctaaat
    16861 aatcttgtta gttcggatac agatttcact aagaatgctg ttgaaggttt tacaagtgtt
    16921 ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag aactacaaat gaagtctgca
    16981 tttggtggaa ctcaacaatc gaataaccag gttcagcaga acaatgaaac attaacaaat
    17041 ccagaagaac aattaggaca aattcttcaa caatttaact aggagagtga aaaattatga
    17101 aaaaaacatc tttaaataat ttagagtatt tggatatttc accagcgatt aatgctatgc
    17161 aagtaccaaa tacacctttt ttaagctatt tacttggtgc tggaaaaaca gagccagcaa
    17221 actcgacaga aattaaatgg cgtgaatatg atatcaacaa cgatgattct tctgaaaaac
    17281 ttgagggcgg agaatatcca gatgctgaat caggtcgaac ttggtttaac aactatactg
    17341 aaatttttag aaaatcaacc tctgtatctg gtacattaga tgctattaat gtgaatggag
    17401 tcggaaatga attaactaat caagtagcac tacgtggtat ggaaatgaaa attgatttga
    17461 accgaaaatt gattactggt gtaaaagctg atgaaaatgg ttctaaaggt cgtcgaatga
    17521 atgggatttt gaacttgatc aattcagcaa ataaggcaga aacagccact gcgggtgcag
    17581 taacaagaaa agatatcgat actttattta aattgatgta tgaaaaaggt tatatgggag
    17641 aaaaactatg cttgatttct ccagatatgc aggagttaat gactgatgag ttagatggaa
    17701 aatcaacaaa aattgttcag ttcggggaaa gagtaacttt tggattgcaa attggaaata
    17761 tcgtgtctaa ttacggtaca ggtattgctc tactagaacc atcattgcca aaaggaacaa
    17821 ttgccgcaat cgatactaat tatgtgaaac tacgtccatt acgtgaatgg agagcagaag
    17881 aacttgcaaa aacaacggat tctagacgta tcggtcttgt aggtgaatac tctcttgaat
    17941 acaatgcttc aaactctggg gcaattttaa atttaaagtc tgaataaaag ggagttagta
    18001 ctcccttttt tgataggagg aattacagtg gtaaaaaaag atgaaactaa aaaagatgaa
    18061 gtcgtgaaat atagagtagg taaaactaaa aattttgttg ggtttgttca tcctaaaact
    18121 cgtagattta tcacagcaga ttcaaataac gaatttatca tttctataga tgataaagaa
    18181 gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa tttaggaagt gatctgatgg
    18241 atgaatcgct aaaaacggaa atcattgagt ctacaaaaga agattttcca gatttgagtg
    18301 aagaacgcat aactaattta ttagaaataa ttttgctaga aattgaatca tacaacactt
    18361 gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat tagtgaagtg ttgtataaaa
    18421 taataaaaaa tgaatcagaa aaaacagtat cttcagttag acgtggcgat acaacgatta
    18481 gttatgcttc aacaacaaat gatgttagtg agttacttct aggctatggt gatttgatac
    18541 gaagagttat tggttgtgga ggattggagt ttttttaatg aatgaagcag atattttaga
    18601 aactacttat gaagatagtt gtattattga aagactgacg gacattgaag attctaatac
    18661 aaatattact attcaagatt acaaaaaagt atatgataat cctatttctt gcgctctttc
    18721 acaaggtcaa atcgatggac tagcagtcat agaagatgga gagatggtaa atgtttcaac
    18781 tgacacatat aaattatttg ttcatcctaa gattaaacta aaaaaaggag atcgaataac
    18841 aataactcaa aaggccagtg gcttaatttt ttctctattt gctactaagc ctttttacta
    18901 tcctagtcat tgtgaagtga atttgatagg aagtgagaaa aatgggtaat ctcaaatatg
    18961 aatcaaatgc agaaaagatc attgagaatt ttaaaaatat gactgtaatt gctcaaaaag
    19021 aaggaatatc cttcgttaac gattcaatga ataaagtcgt tagtttagct aaaccattaa
    19081 ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt agtaaaagct agaaaactat
    19141 caagcggtcg aattgttgga gcagttataa acaatgaaca ttattttaaa tatgtagaag
    19201 aaggtcatag gactaaaaat ggtggctttg tcaaagggaa attcatgtta actcgtgcaa
    19261 cgaatcttgc aaatatgtct tatattcctc gaagatttaa acaaatggca ataaaaatcg
    19321 ttaagaaagg aaagtaacat gaaagatgaa atcattgctg caatcagtag caaattaaaa
    19381 gaaatctatc ctgatgggac aatatatctt gattcagtta tgcagtcaac taaagatttt
    19441 tatttcgttt tatcagtaat ggaatctgga actgaaaatg taggaattga tattcaaaat
    19501 gtttctttct taattgatat tgcattgatt gataataaac ctaatagaaa tttaattaat
    19561 gaattagtct cacgttgtgg gacttttttt aatacgatta caatcgatga gcaaatatta
    19621 tttccaaaag cctatttacc tgatgaagca gatggtgttc aacatattcg ttttacatta
    19681 gaatttccac aatatattga atggagtgaa agataaatgg gagaaaaaag aagtaaaacc
    19741 ggaattattt ctgtcgaaaa acctacttgg tttcctttgg aagatgagac agggaatttt
    19801 ccggtatatg gtactgcgtt gccaatggga acagcagtaa gtatcaaacc aacagctaat
    19861 tatgaaacaa cacaagatta tggggattca gtggttcaag atcaatttac agcttttggt
    19921 ggtgcagaag ttgaactgga agcaaatgga tatactcata aagttttatc tacaattact
    19981 ggagggaaaa ttgttaaagg tggtgccttg cgttctggag aagatattgc gcaagatggt
    20041 gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc gttatacggt cttttataaa
    20101 ggacagtttg ctctagattc agatgaaaca tcaacgattg aaggaagtaa agtaagtttt
    20161 actcatccaa cttggaaggg ttcatttgta gatgtaccag gacttggata tatgtattca
    20221 gttgatgaag atgatgaagg cgtggataaa gcaatgatcg gaaattggtt tacaaaagta
    20281 gcaattccaa ttgaagaagc agaactttca ggaggtacag aataatgtca aaatatcaaa
    20341 caacgattaa attaacgaaa aaagatgaag aaggtaaata tgagcaagta caatttaaat
    20401 ctgctgaatt tctaccaggc ccagtcgtag aagaagcagc agaagtaatg gagggaatgc
    20461 aaaatgctgt agatggaaaa tcagtatcag aagcattaag tcgagcttat tcatttattg
    20521 cagatatttt gtttgaagga cagtttactg gtgaagatta tcgtcaaggg atcgatgctc
    20581 gagaaattgc ttcgttgaca ggtaagttat taaaatctgt tactgcaggc tttgatgaaa
    20641 cttatacaga aacgaaaaaa aagtaagcga agctctcaaa tatccttttt ttaaacattc
    20701 gattacttat cgagaattag atttaaagac gcaattactc gaagcaggtt ggacgttacc
    20761 agaaattgaa cgtactgact ttgacgagtt aatgcgtctt tttgcttttc gagatgcagt
    20821 aaaagagcat gaggatgttg agtactacga taacttcacc caattttagg aggtgatact
    20881 ttgaataacg atgacctaat tctgaagatg atactagatg aatctggttt tactgccggt
    20941 atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa caatcgaaag aaaaagtaga
    21001 aatagtggca gttcattagg tagtatttgg aaaatatttg ctggaagttt tctagctagc
    21061 ggagtaacta gaatcgtagg agctggcttt gatctaatca agggttccat aagtggagcg
    21121 gttgatcgag tagatacgat gaataacgct ctacgaaatt tccaaaatat gggatttagt
    21181 aattcagaaa ttatgaagaa tatcggaaag aatgggcttt tatctcaagg tattcaagga
    21241 cttcctactg ctttgaatga tgcgataagt catgttcaac ttcttgcttc ttctacaggt
    21301 gatttaactc gttcaactca aatatttaaa gctttaaatg atggaattct tggtttcggt
    21361 ggttcaactg atcaagttaa tgaagctgtt attcaattat ctcaaagttt ctcaaacgga
    21421 aaagtagacg cacaaacttg gaactcaatg atcaacgctc aacttggacc tactctttct
    21481 gctattgcta aaaagatggg aattacaatg ggagatctga aagaaggttt atctcaaggt
    21541 aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa tggataccaa aggtggcgga
    21601 ggacttaaat cattaagtca aatcgctaaa gattctacta agggaattaa aacctctata
    21661 caaaatgcaa aaacagctgt tacacgtggt gttggtgagg taatagaagg attaaataaa
    21721 gcattagtag attcagattt aggtggattt aagggaatta ttgataaagt gggcagttca
    21781 atggaatcgt tcttaaaagt aattgctgca aatattccta cagcagtatc atttttaagt
    21841 aatttattta acgaaattca aaaatttggt tctgctttga aattcatgat gccctttctt
    21901 gttcctgcag ctaccgcttt tggagcgttc atgtttcaac ttaaaggtat accagcaata
    21961 ataaaaagct tcaataattt taagaatgcc ataattggcg ttggaaattc attaaagatt
    22021 atgggggcaa tagctgctgc taattcattt gttttaatta ttggtgcagt cgttggtgca
    22081 atagctgttt tcggttactt tatggcaacc aatgaagagc ttagaaacaa ggttatatct
    22141 atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa aaaatataaa ggactgggga
    22201 attgatactt ggaattctgc taaagaaatg gcatcaaatg cagttgaggg tgtcaaagat
    22261 gcttggtcag gaacaaagga atggttttct aacacctgga aaggtattaa ggatggggcc
    22321 acaggtttat ttgataaaac agtagagact tcaagaaatg cagtcgatag tgtaaaaaat
    22381 gcatggtcta atatgaagaa atggttttct gatacttggc aaagtataaa agattcagcc
    22441 agagaaaaat ggaatggaat caaaggttct attatgtcag ttgctggtcc attgattaca
    22501 ggtataaaga atgcattttt acatgttact ttttatctag agaccttatg gaataattta
    22561 gttgagattg gtaaaaacgt ctttgagatt ttaaaaaatg ttatacttgc gccggttttg
    22621 tttattacct ccatgatttc tggtggatgg gaagaaacaa aaaataatat gattgctgtt
    22681 tggaataata ttaaagaaag tgccataaat atttgggaat ctataaagaa tatatttgta
    22741 agttatttta caaatattta ttttgctgca ctcaatattt ggacagggtt caagcttaca
    22801 ttgataaata tttggaatga agtggtaaat caagctaaat cgatttggtt aaacataaag
    22861 tattttttta ttaatctttg gattgatata aaatattttg caattcagaa atggattgaa
    22921 ttgaagtttt ctattattca aacttggatc gatttaaaat ataatgccat tactacttgg
    22981 aataatatta aacagttttt caaagatact tggaaaaata ttaaagatac ggcatacaat
    23041 acgtggattt ctataaaaaa taccatgatt aatacatgga ataacattaa ggactctttc
    23101 tggaatattg ttactggaat tgttaattct gctgaaaatg catggacaaa tctaaaaaat
    23161 ggtgtttcaa atgctattaa tcgggtaaaa gaaatcttta attcgttaag ggaagtcaat
    23221 ttatttgaaa ttggcaagaa catcattgat ggacttataa atggtgtaaa agaaaagtgg
    23281 aatgctttga aagatactat caaaggtatt gcaggtaata tcaaaaattg gattaaagga
    23341 gctttagata ttcattctcc atcaagatgg atgcgtgata tggttggtaa aaatattgtt
    23401 caaggtatta tcgttggtat tgataaagag caaagtaagt tagatcaaac catgacaaat
    23461 ttagtaaaaa caccgtctgt tcaacctgta gtaaatggaa taagtcacct accaattaca
    23521 caaacaaagc aaaatatatc tgctagtgaa acaaaagaaa ttcatttaca tttaaatgtt
    23581 tatggagatt tacctgattc aatggttcga aagattacta caaaaattaa aacagaattg
    23641 acaagacaaa tgaaacgaga tgctggtgca gtaggaggaa cattatatgc aacttaagag
    23701 aggacaattt tttattaatc aacattattc ttctgaattt aatgtgtata ttcaaaacag
    23761 gcctgcttct gtttcagcta gtcgagtaat tgaattgaga gagcgagaag gaaatgattc
    23821 tatcattatt gataaaggct attacaaaaa tgtgactaga aagattgagt gttactataa
    23881 agcaccttca attgatatgg tgcaggagtg ggaagatcga atcactgaat ggttagatat
    23941 gtcgtcttat agtgatttta ttctttacta tgatgaacaa tatatttatc aagctgtagt
    24001 aatagaagga cctgaattta aaggaacaag aaaaacagga aatattgtcc cttttgaatt
    24061 tacagtgagt attcgaccat ttaaggaaaa ttacaatggt agatttacta ttcgacaaac
    24121 agaaactttt gaaatttata acccagagaa gtattcttca aaaccgctta ttaaattgag
    24181 tggttctgga gatgcttctt tttatattaa taaggataaa tatgatttga aatcgttgga
    24241 cagagaatta tttatagatt ctaaattaga agaggcttat cgaaaattag atggtaattt
    24301 agaacatcag gatcaggtca ccttattttt agattttcca tttttatatc ctggaaaaaa
    24361 tgaaattaaa tggacgaaaa atattcattc atttgaaata atgcctaggt ggtggagaaa
    24421 agtatgaaac caagaatata tagtcctact gaaacagatt tctcaacgaa tggcttagga
    24481 attttaaagg attgtacaag atgtgaaata tatgaagtag ctaatggaaa atatgaatta
    24541 gagttggatt atcctttagg aactagattt gatgaatatt ttgaaaatga ctatcaaata
    24601 aaagcaaagc caaacgatca agaagagtat catatctttt ttattgatga taaagatata
    24661 gatacttttt taaatacagt aactatttat gctcagagtc gtacaaatcg acttggaaga
    24721 cgggtagtca ctcttgtgga agtggactct aaaactggtc aagaagcgat atcaattatt
    24781 gaaactaaaa tggataaaaa atctgacata cgactttatt ctgatattac ggctgtttct
    24841 agcacaatct ttgaagcgag aaatgtttta aattgtattg ctggtgaaca aggatcattg
    24901 cttcaatatt ggggcggaga aattaaacgt gaaccattta agctttcttt gttaaagcga
    24961 agaggtcgca ataatgttgg aacgattcgg tatggaaaag atatgtctgg tttgaaggtc
    25021 aaattagatt ggacaggtat aaaaacaaga attattccat atgctgatcc tcaaagtgaa
    25081 gtaggtacga ctaatcgaat ttatggttcg ccagtagata gtgcctatat taataactat
    25141 cctgatgtct atacagaaca tgttcagttt acagaagaac aaggagtaaa agacattaag
    25201 agcttaaata aaatagctaa aaattacttt aaaacgatca atcctggctg tgataagcct
    25261 aaagtttcta ttacggttga atttgataag ttgactgata gtgaagaagc gaaagaattt
    25321 gcgaagatta gaaattatgg tctgtttgat acatttaaaa tatatcataa aaaatataat
    25381 atttatcttg aatcaaaggt tagtggacta caatacgatt ctttatctga aaaaactttg
    25441 aaattagaag caggagacat tcaagttgct ttttatcagc aacaagctgt aactattcag
    25501 gataaattaa aagattatgc aacgaataat tatatgagtg attttaatga ttatgtttct
    25561 tcaatgatta caggtcaagg cagtgcagga ggatatgtag ttttatggcc aaaagaaaaa
    25621 ccttccaaca tctttattat ggataatcca gatttagaaa aggcaagaga agtactaaga
    25681 atgaataaaa atgggattgc tttttctaag aaaggatggc gggggccttt taattcagct
    25741 tggacattag atagtatatt taatgccaat tttatacaaa ctggattaat caaagcagat
    25801 atctttcaaa attcttttaa caagactgga gatgtattga aattagttaa tggactactc
    25861 caaatttgga acaacaaaaa gaaaataatg gagttaacta aaaaaggaat ggaattttgg
    25921 aattctaagg gttcaattgg aacgattgga acaactgatt ctgttggtaa tccttttcct
    25981 ggggcttcta ctccaacccc tattgaagat aattctttag ttattcgtac aaatggagac
    26041 ggcaaatata ttttgatttc tcctaaagta ggaaaaggat tagttttatt aggaaatggt
    26101 aaagcaattt attttggaga cttagatgta caaggaaaac ttacagttaa aggaaaagaa
    26161 ataattcctg gacaaggtgg tggtccatcg ggaggtggag aatctactgg tgggtatcct
    26221 agtgaattaa aaacagatgc agaaaagaga gcttggagaa t
//
LOCUS       DAYYCQ010000038        24261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000038 DAYYCQ010000000
VERSION     DAYYCQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.038"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHAYF_001528"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHAYF_001528"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8578970.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHAYF_001529"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHAYF_001529"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8578971.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHAYF_001530"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHAYF_001530"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8578972.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHAYF_001531"
     CDS             complement(3373..4680)
                     /locus_tag="ACHAYF_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8578973.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHAYF_001532"
     CDS             complement(4683..5264)
                     /locus_tag="ACHAYF_001532"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8578974.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHAYF_001533"
     CDS             complement(5836..7128)
                     /locus_tag="ACHAYF_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8578975.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHAYF_001534"
     CDS             complement(7125..7748)
                     /locus_tag="ACHAYF_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578976.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHAYF_001535"
     CDS             complement(7751..8587)
                     /locus_tag="ACHAYF_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578977.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHAYF_001536"
     CDS             complement(8604..9491)
                     /locus_tag="ACHAYF_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8578978.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHAYF_001537"
     CDS             complement(9507..10790)
                     /locus_tag="ACHAYF_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8578979.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHAYF_001538"
     CDS             complement(11473..12156)
                     /locus_tag="ACHAYF_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8578980.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHAYF_001539"
     CDS             complement(12174..12791)
                     /locus_tag="ACHAYF_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8578981.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHAYF_001540"
     CDS             complement(12801..13625)
                     /locus_tag="ACHAYF_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8578982.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHAYF_001541"
     CDS             complement(13618..14178)
                     /locus_tag="ACHAYF_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8578983.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHAYF_001542"
     CDS             14352..15308
                     /locus_tag="ACHAYF_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8578984.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHAYF_001543"
     CDS             complement(15613..16569)
                     /locus_tag="ACHAYF_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8578985.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHAYF_001544"
     CDS             16673..17275
                     /locus_tag="ACHAYF_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8578986.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHAYF_001545"
     CDS             complement(17363..18862)
                     /locus_tag="ACHAYF_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8578987.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHAYF_001546"
     CDS             complement(18878..19402)
                     /locus_tag="ACHAYF_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8578988.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHAYF_001547"
     CDS             19700..20449
                     /locus_tag="ACHAYF_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8578989.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHAYF_001548"
     CDS             20440..21225
                     /locus_tag="ACHAYF_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8578990.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHAYF_001549"
     CDS             21194..21910
                     /locus_tag="ACHAYF_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8578991.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHAYF_001550"
     CDS             complement(22012..23031)
                     /locus_tag="ACHAYF_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8578992.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHAYF_001551"
     CDS             complement(23050..24051)
                     /locus_tag="ACHAYF_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8578993.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7189 a         5222 c         4225 g         7625 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt a
//
LOCUS       DAYYCQ010000039        23827 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000039 DAYYCQ010000000
VERSION     DAYYCQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.039"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHAYF_001552"
     CDS             complement(53..787)
                     /locus_tag="ACHAYF_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8578994.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHAYF_001553"
     CDS             1114..1524
                     /locus_tag="ACHAYF_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8578995.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHAYF_001554"
     CDS             1517..2218
                     /locus_tag="ACHAYF_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8578996.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHAYF_001555"
     CDS             2218..3804
                     /locus_tag="ACHAYF_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8578997.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHAYF_001556"
     CDS             3823..4044
                     /locus_tag="ACHAYF_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8578998.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHAYF_001557"
     CDS             4041..4802
                     /locus_tag="ACHAYF_001557"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8578999.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHAYF_001558"
     CDS             5182..5691
                     /locus_tag="ACHAYF_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8579000.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHAYF_001559"
     CDS             complement(5766..6305)
                     /locus_tag="ACHAYF_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8579001.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHAYF_001560"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHAYF_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8579002.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHAYF_001561"
     CDS             7318..8169
                     /locus_tag="ACHAYF_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8579003.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHAYF_001562"
     CDS             8197..8832
                     /locus_tag="ACHAYF_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8579004.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHAYF_001563"
     CDS             8852..9625
                     /locus_tag="ACHAYF_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8579005.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHAYF_001564"
     CDS             10033..10830
                     /locus_tag="ACHAYF_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8579006.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHAYF_001565"
     CDS             10808..11221
                     /locus_tag="ACHAYF_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8579007.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHAYF_001566"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHAYF_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8579008.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHAYF_001567"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHAYF_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8579009.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHAYF_001568"
     CDS             15998..16558
                     /locus_tag="ACHAYF_001568"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8579010.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHAYF_001569"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHAYF_001569"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8579011.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHAYF_001570"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHAYF_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8579012.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHAYF_001571"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHAYF_001571"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8579013.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHAYF_001572"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHAYF_001572"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8579014.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHAYF_001573"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHAYF_001573"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8579015.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHAYF_001574"
     CDS             21905..22483
                     /locus_tag="ACHAYF_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8579016.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHAYF_001575"
     CDS             22535..23017
                     /locus_tag="ACHAYF_001575"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8579017.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHAYF_001576"
     CDS             23134..23508
                     /locus_tag="ACHAYF_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579018.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23621..>23827)
                     /locus_tag="ACHAYF_001577"
     CDS             complement(23621..>23827)
                     /locus_tag="ACHAYF_001577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579019.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7781 a         4286 c         5115 g         6645 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DAYYCQ010000040        23666 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000040 DAYYCQ010000000
VERSION     DAYYCQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.040"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..120)
                     /locus_tag="ACHAYF_001578"
     CDS             complement(1..120)
                     /locus_tag="ACHAYF_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8579020.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            299..1960
                     /locus_tag="ACHAYF_001579"
     CDS             299..1960
                     /locus_tag="ACHAYF_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8579021.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2392)
                     /locus_tag="ACHAYF_001580"
     CDS             complement(2030..2392)
                     /locus_tag="ACHAYF_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8579022.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2460..3401
                     /gene="opp3b"
                     /locus_tag="ACHAYF_001581"
     CDS             2460..3401
                     /gene="opp3b"
                     /locus_tag="ACHAYF_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8579023.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3402..4445
                     /locus_tag="ACHAYF_001582"
     CDS             3402..4445
                     /locus_tag="ACHAYF_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8579024.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4451..5521
                     /locus_tag="ACHAYF_001583"
     CDS             4451..5521
                     /locus_tag="ACHAYF_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579025.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5511..6464
                     /locus_tag="ACHAYF_001584"
     CDS             5511..6464
                     /locus_tag="ACHAYF_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579026.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6476..7486
                     /locus_tag="ACHAYF_001585"
     CDS             6476..7486
                     /locus_tag="ACHAYF_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8579027.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7771..8592)
                     /locus_tag="ACHAYF_001586"
     CDS             complement(7771..8592)
                     /locus_tag="ACHAYF_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8579028.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8689..8967)
                     /locus_tag="ACHAYF_001587"
     CDS             complement(8689..8967)
                     /locus_tag="ACHAYF_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579029.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9235..11244
                     /gene="metG"
                     /locus_tag="ACHAYF_001588"
     CDS             9235..11244
                     /gene="metG"
                     /locus_tag="ACHAYF_001588"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8579030.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11723..12310)
                     /locus_tag="ACHAYF_001589"
     CDS             complement(11723..12310)
                     /locus_tag="ACHAYF_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579031.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12485..13807
                     /locus_tag="ACHAYF_001590"
     CDS             12485..13807
                     /locus_tag="ACHAYF_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8579032.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13962..14738
                     /locus_tag="ACHAYF_001591"
     CDS             13962..14738
                     /locus_tag="ACHAYF_001591"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8579033.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14731..15300
                     /gene="rnmV"
                     /locus_tag="ACHAYF_001592"
     CDS             14731..15300
                     /gene="rnmV"
                     /locus_tag="ACHAYF_001592"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8579034.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15293..16177
                     /gene="rsmA"
                     /locus_tag="ACHAYF_001593"
     CDS             15293..16177
                     /gene="rsmA"
                     /locus_tag="ACHAYF_001593"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8579035.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16628..17257
                     /locus_tag="ACHAYF_001594"
     CDS             16628..17257
                     /locus_tag="ACHAYF_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579036.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /locus_tag="ACHAYF_001595"
     CDS             complement(17381..18490)
                     /locus_tag="ACHAYF_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579037.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18699..19121
                     /gene="mgsA"
                     /locus_tag="ACHAYF_001596"
     CDS             18699..19121
                     /gene="mgsA"
                     /locus_tag="ACHAYF_001596"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8579038.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19308..19406
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19444..19980
                     /locus_tag="ACHAYF_001597"
     CDS             19444..19980
                     /locus_tag="ACHAYF_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8579039.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20020..21783)
                     /locus_tag="ACHAYF_001598"
     CDS             complement(20020..21783)
                     /locus_tag="ACHAYF_001598"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579040.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21786..23501)
                     /locus_tag="ACHAYF_001599"
     CDS             complement(21786..23501)
                     /locus_tag="ACHAYF_001599"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579041.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7633 a         4322 c         4801 g         6910 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt tttcatttga ttgctccttt atttttggtt
     2401 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2461 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2521 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2581 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2641 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2701 ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2761 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2821 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2881 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2941 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     3001 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     3061 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     3121 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     3181 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3241 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3301 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3361 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3421 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3481 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3541 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3601 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3661 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3721 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3781 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3841 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3901 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3961 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     4021 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     4081 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     4141 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     4201 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4261 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4321 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4381 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4441 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4501 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4561 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4621 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4681 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4741 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4801 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4861 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4921 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4981 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     5041 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     5101 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     5161 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     5221 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5281 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5341 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5401 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5461 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5521 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5581 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5641 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5701 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5761 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5821 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5881 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5941 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     6001 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     6061 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     6121 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     6181 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6241 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6301 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6361 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6421 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6481 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6541 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6601 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6661 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6721 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6781 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6841 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6901 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6961 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     7021 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     7081 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     7141 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     7201 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7261 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7321 ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa
     7381 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7441 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7501 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7561 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7621 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7681 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7741 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7801 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7861 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7921 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7981 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     8041 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     8101 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     8161 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     8221 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8281 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8341 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8401 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8461 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8521 aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc
     8581 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8641 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8701 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8761 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8821 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8881 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8941 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     9001 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     9061 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     9121 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     9181 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9241 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9301 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9361 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9421 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9481 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9541 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9601 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9661 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9721 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9781 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9841 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9901 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9961 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
    10021 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
    10081 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
    10141 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
    10201 cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt
    10261 gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca
    10321 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10381 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10441 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10501 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10561 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10621 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10681 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10741 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10801 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10861 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10921 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10981 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    11041 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    11101 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    11161 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    11221 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11281 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11341 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11401 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11461 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11521 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11581 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11641 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11701 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11761 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11821 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11881 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11941 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    12001 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    12061 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    12121 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    12181 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12241 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12301 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12361 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12421 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12481 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12541 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12601 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12661 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12721 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12781 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12841 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12901 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
    12961 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    13021 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    13081 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    13141 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    13201 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13261 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13321 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13381 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13441 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13501 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13561 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13621 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13681 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13741 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13801 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13861 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13921 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13981 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    14041 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    14101 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    14161 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    14221 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14281 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14341 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14401 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14461 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14521 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14581 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14641 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14701 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14761 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14821 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14881 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14941 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    15001 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    15061 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    15121 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    15181 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15241 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15301 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15361 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15421 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15481 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15541 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15601 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15661 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15721 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15781 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15841 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15901 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15961 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    16021 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    16081 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    16141 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    16201 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16261 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16321 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16381 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16441 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16501 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16561 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16621 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16681 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16741 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16801 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16861 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16921 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16981 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    17041 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    17101 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    17161 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    17221 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18541 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18601 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18661 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18721 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18781 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18841 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18901 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18961 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    19021 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    19081 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    19141 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    19201 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19261 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19321 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19381 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19441 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19501 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19561 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19621 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19681 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19741 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19801 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19861 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19921 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
    19981 aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc
    20041 ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc
    20101 gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg
    20161 atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt
    20221 tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt
    20281 actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc
    20341 ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata
    20401 agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag
    20461 attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt
    20521 acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt
    20581 tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt
    20641 tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg
    20701 ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt
    20761 gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc
    20821 aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc
    20881 gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt
    20941 gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc
    21001 aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg
    21061 tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg
    21121 ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc
    21181 cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact
    21241 cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg
    21301 catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc
    21361 cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc
    21421 gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt
    21481 tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg
    21541 actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt
    21601 gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat
    21661 cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc
    21721 ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt
    21781 cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt
    21841 aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt
    21901 gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc
    21961 tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct
    22021 aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt
    22081 ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga
    22141 cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg
    22201 tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg
    22261 ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg
    22321 tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc
    22381 gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat
    22441 gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa
    22501 gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa
    22561 ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc
    22621 acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa
    22681 gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc
    22741 aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata
    22801 ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt
    22861 tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg
    22921 tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg
    22981 atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact
    23041 tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc
    23101 aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg
    23161 gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt
    23221 ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat
    23281 tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt
    23341 tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt
    23401 aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt
    23461 tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt
    23521 atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga
    23581 aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt agaaattgaa
    23641 tagactattt tttttaaaaa aaagaa
//
LOCUS       DAYYCQ010000041        23215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000041 DAYYCQ010000000
VERSION     DAYYCQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.041"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..68)
                     /locus_tag="ACHAYF_001600"
     CDS             complement(<1..68)
                     /locus_tag="ACHAYF_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8579042.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWL"
     gene            341..2812
                     /locus_tag="ACHAYF_001601"
     CDS             341..2812
                     /locus_tag="ACHAYF_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8579043.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2915..4651)
                     /locus_tag="ACHAYF_001602"
     CDS             complement(2915..4651)
                     /locus_tag="ACHAYF_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8579044.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4648..5352)
                     /locus_tag="ACHAYF_001603"
     CDS             complement(4648..5352)
                     /locus_tag="ACHAYF_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8579045.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5483..5986
                     /locus_tag="ACHAYF_001604"
     CDS             5483..5986
                     /locus_tag="ACHAYF_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8579046.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5946..6284
                     /locus_tag="ACHAYF_001605"
     CDS             5946..6284
                     /locus_tag="ACHAYF_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8579047.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6305..7723
                     /gene="ffh"
                     /locus_tag="ACHAYF_001606"
     CDS             6305..7723
                     /gene="ffh"
                     /locus_tag="ACHAYF_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8579048.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7835..8413
                     /locus_tag="ACHAYF_001607"
     CDS             7835..8413
                     /locus_tag="ACHAYF_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8579049.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8417..8938
                     /locus_tag="ACHAYF_001608"
     CDS             8417..8938
                     /locus_tag="ACHAYF_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8579050.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9017..9571)
                     /locus_tag="ACHAYF_001609"
     CDS             complement(9017..9571)
                     /locus_tag="ACHAYF_001609"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8579051.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9824..10099
                     /gene="rpsP"
                     /locus_tag="ACHAYF_001610"
     CDS             9824..10099
                     /gene="rpsP"
                     /locus_tag="ACHAYF_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8579052.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10111..10362
                     /locus_tag="ACHAYF_001611"
     CDS             10111..10362
                     /locus_tag="ACHAYF_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8579053.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10487..11278
                     /locus_tag="ACHAYF_001612"
     CDS             10487..11278
                     /locus_tag="ACHAYF_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579054.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11448..11969
                     /gene="rimM"
                     /locus_tag="ACHAYF_001613"
     CDS             11448..11969
                     /gene="rimM"
                     /locus_tag="ACHAYF_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8579055.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11959..12720
                     /gene="trmD"
                     /locus_tag="ACHAYF_001614"
     CDS             11959..12720
                     /gene="trmD"
                     /locus_tag="ACHAYF_001614"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8579056.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12856..13203
                     /gene="rplS"
                     /locus_tag="ACHAYF_001615"
     CDS             12856..13203
                     /gene="rplS"
                     /locus_tag="ACHAYF_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8579057.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13576..14238
                     /locus_tag="ACHAYF_001616"
     CDS             13576..14238
                     /locus_tag="ACHAYF_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579058.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14317..17544
                     /gene="fss3"
                     /locus_tag="ACHAYF_001617"
     CDS             14317..17544
                     /gene="fss3"
                     /locus_tag="ACHAYF_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8579059.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17658..17972
                     /locus_tag="ACHAYF_001618"
     CDS             17658..17972
                     /locus_tag="ACHAYF_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8579060.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17985..18359
                     /locus_tag="ACHAYF_001619"
     CDS             17985..18359
                     /locus_tag="ACHAYF_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8579061.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18369..18713
                     /locus_tag="ACHAYF_001620"
     CDS             18369..18713
                     /locus_tag="ACHAYF_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8579062.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18784..20136
                     /locus_tag="ACHAYF_001621"
     CDS             18784..20136
                     /locus_tag="ACHAYF_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8579063.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20196..21338
                     /locus_tag="ACHAYF_001622"
     CDS             20196..21338
                     /locus_tag="ACHAYF_001622"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM8579064.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21363..21617
                     /locus_tag="ACHAYF_001623"
     CDS             21363..21617
                     /locus_tag="ACHAYF_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579065.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21873..23057
                     /gene="mobT"
                     /locus_tag="ACHAYF_001624"
     CDS             21873..23057
                     /gene="mobT"
                     /locus_tag="ACHAYF_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8579066.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23054..23191
                     /locus_tag="ACHAYF_001625"
     CDS             23054..23191
                     /locus_tag="ACHAYF_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8579067.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7714 a         4123 c         4989 g         6389 t
ORIGIN      
        1 agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt tctagtatga
       61 ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat tttaccaaaa
      121 gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt
      181 tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg aatgaaaggt
      241 gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt
      301 ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa
      361 tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc
      421 atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc
      481 aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt
      541 tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta
      601 taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga
      661 aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt
      721 catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt
      781 tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag ggaaactgca
      841 agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa taggtgatcc
      901 attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg
      961 agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca
     1021 gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc
     1081 tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag
     1141 tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac
     1201 gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa
     1261 tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac
     1321 gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat
     1381 cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga
     1441 caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca
     1501 ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt
     1561 gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga
     1621 gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat
     1681 tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa
     1741 tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat
     1801 caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat
     1861 ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc
     1921 tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca
     1981 attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg atattctagc
     2041 catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg ccatgcaggc
     2101 ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg ttgcagaagg
     2161 acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga agaatatttt
     2221 ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt tagaaccatc
     2281 acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc ttgctctaga
     2341 agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa aagctgtgcc
     2401 aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa
     2461 tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag tagggttcct
     2521 cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca ttcttttctg
     2581 tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa tcaccggtat
     2641 ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt cgatttttag
     2701 gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc
     2761 gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt gacgaaaagt
     2821 atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca ggatgagtca
     2881 tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg gtaacggtga
     2941 aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg agttgcacgt
     3001 agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt cgcgccttgt
     3061 ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata cctataccaa
     3121 agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata atctcgctgt
     3181 cgctttttga atattggaca gcattttcaa ttagattttt gataattgga taaaggatat
     3241 ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac cgtttctttt
     3301 cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta taaattgtct
     3361 ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga atcagtttat
     3421 ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt tgcgggtcat
     3481 cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca ggagttttta
     3541 attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt cgcactttcg
     3601 ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta aagtagcgca
     3661 aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg atcagcggat
     3721 gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg acttctgtgt
     3781 accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt ggattaagca
     3841 gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt tcgttcagta
     3901 acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc atttgttcgc
     3961 tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc atgattactt
     4021 ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt ttcaatggct
     4081 ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag aaagacagaa
     4141 agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaccg cttgtcggtt
     4201 ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat gcgacatata
     4261 aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc ccaccattca
     4321 atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca ttgtttgtgt
     4381 caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac tgatggacga
     4441 attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct aattgccgga
     4501 tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa
     4561 aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga ataagaaaga
     4621 agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact tatagccaaa
     4681 tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt tttctcgcaa
     4741 atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg
     4801 gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa
     4861 ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt gataattggt
     4921 caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat cttttgattg
     4981 cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt cgcgaggact
     5041 aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc gatccacttg
     5101 gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg
     5161 agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga aatcaaatga
     5221 ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta ccttgtagcc
     5281 ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat caacaactag
     5341 tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt ttagcataga
     5401 atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag gcactttgct
     5461 gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg gtctggcctg
     5521 tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg aaactggaga
     5581 agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag atcccgctga
     5641 aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag
     5701 cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca
     5761 ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc
     5821 gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg ctactgctta
     5881 ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga
     5941 gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt tattcgacct
     6001 tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat ttttcattag
     6061 gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat atcaagcgga
     6121 caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat tatattgttc
     6181 gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg gaagataaaa
     6241 aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa ggaatgaaga
     6301 aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa aaataagaaa
     6361 aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt
     6421 actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg tacgcgaacg
     6481 tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg taaaaatcgt
     6541 tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata aatcaccaaa
     6601 aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga cctttactgg
     6661 taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga
     6721 cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc
     6781 tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag ggatggagtt
     6841 agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc tgcatatcga
     6901 tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa atgagatttt
     6961 gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata gcttcaacca
     7021 gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc gaggcggggc
     7081 agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa
     7141 attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc ttggtatggg
     7201 ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa aagcggaaga
     7261 acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg aacagcttga
     7321 tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag gaatgaatca
     7381 agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat
     7441 ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc caagccgtcg
     7501 tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca
     7561 attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga acatcccagg
     7621 tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg
     7681 aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt
     7741 cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt tattctttac
     7801 taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga gtgaacaaac
     7861 agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg atgaccaaaa
     7921 gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc agacgatttt
     7981 aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa
     8041 ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag
     8101 aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg
     8161 cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt cttcttatca
     8221 ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa aagatctaaa
     8281 gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag aagctgtcgg
     8341 gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag
     8401 aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt tagatggcta
     8461 tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag gtaagcaaac
     8521 tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa aaacttatca
     8581 ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga atttcgtttt
     8641 ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag atcctgttgc
     8701 ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg
     8761 attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc agattacccc
     8821 tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag cgcgttttga
     8881 aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac gaaagtagaa
     8941 gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt tgcctttttt
     9001 attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac gcatctaaaa
     9061 tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca attagattcg
     9121 caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa aagacaagtg
     9181 attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat gcagtaacga
     9241 ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct acatctaaag
     9301 catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa acgacaccgt
     9361 ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt tctggagttt
     9421 cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt tcgtaccctt
     9481 caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct tttcttaaac
     9541 taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa actatctata
     9601 ataatcttac aaaagataag taactggagc aaataaaaac acttactgac aaaagctaca
     9661 tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa
     9721 aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt
     9781 acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag ttaaaatccg
     9841 tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg
     9901 ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc
     9961 tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag gtgcacaacc
    10021 ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc
    10081 taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt agtcttaaca
    10141 atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa
    10201 gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat cattggaaaa
    10261 caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat caatgcaccg
    10321 aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt gctgtactcg
    10381 aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt atttggcaaa
    10441 acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga gaaagaaaat
    10501 gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc aagaatcaga
    10561 caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa aaagtagtac
    10621 caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga aaaagactgc
    10681 tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg taccagtctc
    10741 ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta ttttatatta
    10801 cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc
    10861 aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc
    10921 atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca cagggtatca
    10981 gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc gtattcgaat
    11041 ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa tcgtagctta
    11101 tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg ttgatatggg
    11161 tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat
    11221 cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa
    11281 tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa tcaaaaatag
    11341 cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag aattgcctct
    11401 tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg actgaatact
    11461 tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt gttatctcaa
    11521 caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta ttccaagacg
    11581 gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat tttgacttat
    11641 tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat ggcatattaa
    11701 aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac catgagatca
    11761 tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa atcttgtcac
    11821 caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata ttgcttccat
    11881 atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt gtagaaattc
    11941 cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag aatgtttgaa
    12001 ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt agatatcaat
    12061 atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga ttatccatac
    12121 ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat ccaagccatt
    12181 gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc tggtaaacga
    12241 ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt catttgtggt
    12301 cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt ttccttagga
    12361 gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc aacagttcgg
    12421 cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta ttcaaccggt
    12481 ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa agtaccagaa
    12541 gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga atcactaaga
    12601 aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc tcaaatgacg
    12661 aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga agccaagtga
    12721 ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta tggtgagtgc
    12781 aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa tatttggaat
    12841 aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca attacgttct
    12901 gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt
    12961 actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg tgctggaatc
    13021 agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg tacattccca
    13081 ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca
    13141 aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga aatcagaaga
    13201 taaacacgga aaacttcctt ggaaatctga aatttgtgaa aagtccttgt atatcaaggg
    13261 ctttttcttt tttctctatc acagttttaa gccatttttg gctgttttgg accatttttc
    13321 aatcatattc ccttaaaaat tgtcctagat tgtccgacat tgactatcag tgtcgtcaaa
    13381 atagggcaaa atttagtagt cagcttgacg acaaatataa tacgaaaaat accaaaacat
    13441 ttaccgaatt tagcaacttt tgaacccaag tttctcaaag ggtcagctat tagcaaaaaa
    13501 gacaaaacat gtacttttct actaaaaagt tgatgtgata ggcttctatc taaataacta
    13561 ttaggagttt ttatcgtgaa gcaaaatgat ggacgtatca tttggaaaaa tcaaagtgag
    13621 ttaaaactga ttttaacaat taatgaattc atcgaaaaac acggaattac atctagccgt
    13681 caataccaga aaaagctatc agaaaatcca aattctgcac caagtatgtg gttcattaac
    13741 aaaaaatatg gatcatggga gaatttattg attagtatag gacgagaaaa tactgggtat
    13801 ggaaaatggg ctagaatgtc agaacaagaa ttattggaaa tagtagaagc ctttattaag
    13861 tgcgagaaga taacctctca acgaatgtac gaacaaaaat cagtagggaa gaatattcct
    13921 tcacttagta ccattaaaaa gatgttagga gatatacgtc cactttttaa agaaaaaaat
    13981 gatggatctc gatttactga tttcgaactg ctgttagaac taaaaaatga aataatccga
    14041 ttaaaactac aggatgattt atcaatgaca aagtttcgaa aactagttca atccccaaaa
    14101 ctaccatcag ttgatacaat tatgaaaaga acgaacaaaa attgggagga attgatggca
    14161 gagattggat ttgattatcg aagaatcaaa atttataagc agagaaacaa cctatccaag
    14221 acaaagaaaa ctaaatagta tcactctaaa tcagacgttc aaaattaaaa tttggacgtc
    14281 ttttttatta cccaaaaacg aaaggagatt tattttatga aaaagccaag atttaagaat
    14341 tggcgtttgc tcgcaacact gagtctatta tgccaaacaa ttggtggatc gcttggccca
    14401 acgattgcct ttgctgatga aattactcat ccacaaacgg taactgtgga attggatttg
    14461 gctcaccagt atgctgtcga aggtacattt agcgatggtc gtcccatgtc agaagttacc
    14521 gttccgcact atgcagttta caatggtgtg aagcaagata ttttctgtat tgagccaggg
    14581 gtgccgattt acaatgagtt cactcctggt tatgagaaaa atccattgcc tgatatgtcg
    14641 gagaaagcga aattagtttc cgttttatgg aaggaagctg gtacggatgt ggatacacat
    14701 attgtggccc agaagatgat ttggcaagaa gttaatggct acacgcttca ctcaattaaa
    14761 cgttctgatg gtagtgcagt aaatatcgca gccattgagt caaaaatcaa tcaagccatt
    14821 gcggattatc aaaagaaacc aagcttccat aactccacag tgaaaaccgt gttaggtcaa
    14881 tcgactactg tgacagatac gaacggcttg aatttatcgg agtttgatga agttgtcgag
    14941 aatatcgcaa atattgatta tcgtgtgaac ggcaatcaac tagtgattac tccaaatgcc
    15001 aattcaaaag agagtggtga attaacccta aagaagtcag ctaatacagg aacaccggta
    15061 gcttataaaa tggctggtca acaaactttg atggcggggg caattgataa gcccaatacc
    15121 tacacaatca aaattgatgt ggaaactgag ggttctttga agattaaaaa agtcgataaa
    15181 gaaacaggaa atgttgtacc gggaaccgtt ttccatttag actttggaaa aacattacct
    15241 gcaaaagacg tgaccaccga taaagaaggc attgcgacat tggatgagat tcctcatggc
    15301 gcgaaagtaa ccattactga aaaatcagtg ccagctcctt atacgattga cactacacca
    15361 atcaccgcaa ccattaaatc tggtgagact atttctgtga cttcaaaaaa tactcgcgaa
    15421 aaaggtcaaa tcattttgaa taaaatgggg gtggaaacag gaactgatct ttggaacgac
    15481 aaatactcat tagccggaaa tacgtttgcc attcgcaaag acagtccaac cggtgaaatt
    15541 gtccaagaaa tgaccacgga tgaaaaaggt catgcggaaa caccaaaaga gattgccaat
    15601 gcgttggaat tgggaaccta ttatgtgacc gaaaccaaag ctagtaatgg ctttgtgaat
    15661 acctttaaac cagtaaaagt cgaattgaac tatgtcaatc aaaccgtggc tcttgttacc
    15721 agtaacgtaa aagggcaaaa ccaagaaatc actggtgaaa ccactttgac aaaagaagac
    15781 aaagataccg gtaatgaaac tcaaggtaaa gctgagttta aaggagctga atatactctc
    15841 tttactgcaa aagatggtca agcggtcaaa tggagtgagg cttttaaacc agagttagtg
    15901 aagggaacga aagcttctga tgaaacggtg actttgactt tagatgaaaa gaatcaagtt
    15961 gctgttaaac acttagcgat taatgagtat tactggcaag aaaccaaagc acctgaagga
    16021 tataccttgg atgaaacaaa gtaccctgtt tccatcaaaa aggtggatga taacgaaaaa
    16081 aatgccgtga ttacccgaga tgttacggca aaagaacaag tcattcgctt tggctttgat
    16141 ttctttaaat ttgctggatc agctgctggg actgccgaaa ctggcttcaa tgatctgacc
    16201 tttaaagtat cgccattgga aggaaccagt gagattacag gtgccgaaga tgaagcaatt
    16261 acagcttata acgagcaact aggttttgat ggctatggca agtttgaaaa tcttccttat
    16321 ggggattatt tacttgaaga agtagaggct ccagaaggat ttcaaaaaat tacgccatta
    16381 gaaatccgtt ctacttttaa agaaaataaa gcggattaca ctaagagtga atacattttt
    16441 acgattaccg aaaaaggaca aaaacaacca attaaaacgg tgaccgttcc ttacgagaaa
    16501 ctgacgaata aagcattttc tgttagcttg aatcgtttga tgctctatga tttacctgaa
    16561 gaagaagata gcttaacttc tcttgggact tggaaagacg gaggcaaaga attgacagac
    16621 cttgattcta ccgagctagt tgataaattg agctataact tgcatgaaat caaagaagac
    16681 tggtatgtgg tagcgcaagc cattgatgtg gaagcgacaa aagctgccca agaaaaagat
    16741 gaaaaagcca aaccagtggt aattgccgaa acaaccgcaa cgttggcgaa taaagagaaa
    16801 acaggaactt ggaaaatttt gcataaatta accgcagaac aagttttgga taaaaccatc
    16861 gtcttattca actatgtgta tgaaaacaag aaaccctatg aagcaggtga tgatccagta
    16921 gcgaaagatg ctagcttgaa caatcaagcc caaactgtca attgtacggt ggaacgccat
    16981 gtttccatcc aaacaaaagc ccacctagaa gatggttcgc aaacctttac tcatggggat
    17041 gtggtggata tgttcgatga tgtgtcgatt acccatgatg tactggatgg ctcaaaagaa
    17101 gcttttgaaa caattctgta tgcattactt ccggataata cgaccaaaga aatttggaaa
    17161 tctggcaaaa ttgattatga agtgaatgac aaagaattca ctaaaaccgt acttgcggaa
    17221 aaagtggaca caagtaaata tccagaaggt acaagtttta ccttcaaaga aattaactat
    17281 ggcaaagacg gaaacatcaa tgggaaacac aatgaggatc taaaagaaaa aactcaaact
    17341 ttaacaccta aaaaagttcc gacaacacca agtgcaccag aacaaccgga aataccaact
    17401 gttccaagtg actctcaaga atctagtcct acagtaaaga cattcccaca aacaggggag
    17461 aaaaattcta atgttctgct gtttattggg tttaccttga tctttgcgac tgctgggtat
    17521 tatttctgga atcgccgaaa ctaaggtgat gtgatgaaac aactaaatag gaaaaaccaa
    17581 cgtgcgcgac cgcctcctaa aaaaagtggt cgcgctattt ttgtatccaa aactaaaaat
    17641 gaaaagagga aaagaagatg gaattgaaat ttgttgtgcc agatatggcc gaaacctttg
    17701 gaaagataag gtatgccggc gaaggcgaag ttttaactga aggatatggg cggaatacta
    17761 cagtaattgg tcgtagttat catttgtatt ccagtaagca acgagccgat gatattgaag
    17821 tggtggtagc tgcggaagcc ggcgaaaagg attttgatca agatcaacca ctaaaagccg
    17881 tgaatcccca tttggttgcc aaaggctatg agattgagaa tcgtgggttt accgactacg
    17941 tgttgtatgt cgatgattta gtgaaagcat aggaggaaaa gaagatgaga ttaacagaag
    18001 gtattgttgt agattcagga ctaacgtttg ggaaattacg attttccgca ttacgccgag
    18061 aagtacggaa gcaaaatgag gatggaacgg ttagtaacga ggtaaaagaa cggacttata
    18121 atttgaaatc ctctgcgcaa ggtcgaatga tccaagttag tatcccagca aatgtaccct
    18181 tacgcgagtt tgcctatgac gcagaggtag agttggtcaa tccaatcatg gatacggtcg
    18241 ccaattatgt tttccgagaa ggaactactg tcaattggtt cattaaggcc gatgatttag
    18301 tcttgaaacg acaaccagat cagggaaatt ctacaaacca aaatgaagga aagaaatagg
    18361 tgaagcgtat ggaaattaat gttgaacata ttttagattg tctcgatcaa tatggaaaag
    18421 gtgagctaac ggaggaacaa ctcactaaag cattaaccta tgatgaaaaa atgtttctga
    18481 ttatgcatca agggttactg gaagtaggta acgatgtcga agaagatttt gatgtactga
    18541 attggttagg ggaagaagag tccttcttta tggttatcga agtaaatgaa agtctatgtc
    18601 gagaagccga aaccgtattg gaagaaattg gtgtggaaat gccggatgcc attgaagttt
    18661 ttttaaagca actagtggaa acgaaacaac tccctatagc agtaaatgac tagcataact
    18721 gcttgaaatt ggatcaatga aatcctatga agcttttttc gattggaggt ggaaaccata
    18781 aaaatgtaca aagggcatcg tatacgggca ggagatcagc acttggttta tcactttgtt
    18841 cttggctggc tactcgctct gttcatcggt tggatgagcg tcttttattt tcaagagctt
    18901 agacaatttg atatttctaa gctatcactt tctactatag aaatcgttcg ttctataaaa
    18961 gatttaatct atttgctagg aagtctcgtt ctttcaggaa gcacgatgtt gctttatata
    19021 cacttttttc aggatcattg gcgcagtttg tggcatcgac aaaagctagc ccggatgatt
    19081 ctagaaaatc attggtacga agtaaaacaa actcagagtg aaggattttt caaagacctc
    19141 aatagtagtc gaaccaaaga gacaatcagt tacttcccca aaatctatta ccgtatgaag
    19201 gatggtttac tttctattcg cgttcaaatt tcactgggaa aatatcaaga tcagctttta
    19261 aagctggaaa agaagttaga aagtggtcta tattgtgaac tagtagaaaa ggaactcaaa
    19321 gactcctatg tggagtacac tttactgtat gatataattg ccaatcgaat tgggatagac
    19381 gaagtagtgg cagaaagtgg ggctttgcga ttgatgaaaa atcaagtatg ggcttatgat
    19441 tctttacctc atatgttaat cgctggcggc acaggtggtg ggaagactta ctttttactc
    19501 actatcatcg aggctttatt aaaatctgat gccgagcttt ttgttcttga tcccaaaaat
    19561 gcggacttag ccgatttagg tacggtaatg cctcatgtct attcccaaaa ggaggaaatt
    19621 tctgcttgtg tggaagattt ctatgagcgc atgatgactc gtagcaaggc aatgaaagaa
    19681 atgcccaact acaaaacagg agagaattat gcgtttcttg gacttccacc aaacttttta
    19741 atctttgatg aatacgtggt ttatatggaa atgttaacga cgaaggaaag tgcggtgatt
    19801 ttgaataagc tcaaacaaat cgtgatgtta ggtcgtcaat ctggtttctt tctgattcta
    19861 gcttgtcaaa gaccggatgc gaaatattta ggagacggga ttcgcgatca atttaatttc
    19921 cgggtggctt tgggtcgtat gagtgaactg ggatattcca tgatgtttgg cgaggttgat
    19981 aaaaaattct ttatgaagcg tatcaaaggt cgaggctatg tagatacggg agggagtgtg
    20041 attagtgagt tttatacgcc acttgtacca aaaggatatg atttcttaag ggaaattagc
    20101 gacattggtg gattaaacgt taatacggaa ggataaaata atagtatgta gcctaggagt
    20161 gtcaagaatc ctgggctttt tttacgaggt gatttatgac atttttagat ttattcgctg
    20221 ggattggtgg cttttgctta ggtatggaac aagcgggtca ccagtgtatc ggcttttgtg
    20281 aaattgatga ttttgctcgc cagagttata aggcaatcca tgatacaagt aaggaggtgg
    20341 aaatgcatga catcacaagt gtatcagacg agtttattca atctctcgga ccagtggata
    20401 tcctttgcgg tggatttccg tgccaagctt tttcaattgc gggaaagcgg caaggatttt
    20461 ccgatactcg aggtacttta ttctttgaaa tcgctcggtt cgccgctatt ctccaaccta
    20521 agtttttatt ccttgagaac gtccggggat tactcaatca cgaaggaggg gctacgttcg
    20581 agacgatcct ccgaacgctg gatggattgg ggtatgatgt ggaatggcaa gtgcttaact
    20641 caaaggccta cgttccccaa aaccgtgagc ggatcttcct tatcggacat tctcgagacg
    20701 catgtaccga acaagtattt cctatcattg gatcgtctcc aacatctgat caaaacatca
    20761 gaaacttatt aaatatcaac ccttccaatc gaggaatggg gggacaagtc tatgggtcag
    20821 atgacgtagc gcctaccttg accggtgatg aaggaatcaa aatcgcattg ccggtagatg
    20881 atggaatttc tgtagcgggt atgttactgg gaaattttga acaaggcaat cgggtttatg
    20941 agataactgg aacagcgcct actttgtcaa cgaaacaagg cggaacgaag atcatgcttc
    21001 ggaaaaacga aacagaatat caagaggtga agccagggaa cagtgtgaac ctggcttttc
    21061 ctaattccac aagtcgtcga ggccgtctgg gaaaacaaag tgtgcatacc ttattaacag
    21121 gagaccaaca agcagtcgtc acggatcagt atcaaattcg aaagttaaca cctagggagt
    21181 gctggcggct tcagggcttt ccggactggg cttttgatcg agcttctcaa gtcaattcag
    21241 atagtcagtt gtataagcag gctggaaatt ctgtaacagt tccggtcatt tttgatattg
    21301 ctagaagatt aaaggaggta aaaacagatg atttatgatg agttgattgg ggaatctact
    21361 gggtgattgg caagattcag tcagatccgg aattggaaga agaactgtac cggctcaatt
    21421 ttgatattcg caaaaacggg gtgaaagtcc ctgatgatcc gtatgtaatt gatgaggaaa
    21481 cggatgctcg aatggagctg aatcaagtca tcagtgaatt ggaacgaatt gcggactttg
    21541 ctaaagaacc tgatatccgc caatacttgt ttgagataaa agcagagttg gaaattgagg
    21601 gtattaccgc ggagtaacgg ccaaataaac cactgatagg cactgaccgc gggggatttg
    21661 cgaagcagaa atcctccgtg gtgctgtgcc tgtttatgga cggcgttagc cggccatgat
    21721 gtctcacccc ccgtatctaa caggggggta caaaaacaca aaaaataagc aagcaacgat
    21781 aaggaatggc tgtcctatca aggataataa gcaatttgtg tagatgttaa ctgatttgaa
    21841 aaagttaaca ccttaaattt ggaggtgaga acttggcaca aaggaattta gattatcggc
    21901 tattaaaaga ccggcgtaat gaatatggcg tttcacaaaa taaattagct acgacttgtg
    21961 gacttagtcg cccgtactta aatcagatag aaaatggtgg cgtaaccgca tcgacaaaaa
    22021 ccatgaggaa aatttttgac caactggaaa gtttcaatcc cgagttacct ttaacgttac
    22081 tgtttgacta tgtaaggatt cgctttccca caacggatgc gaggaaaatc attcaagaga
    22141 ttctccattt gaaatttgat tatatgctcc acgaagatta cgccttttac tcctatcaag
    22201 aacaatatgt tatgggagat attgtggtga tgttgtcaca tgaagaagat aaaggcgtcc
    22261 ttttagaatt aaagggtcgt ggttgtcggc agtttgaaac ttttttactc gcccaaaagc
    22321 gtagctggta tgactttttc gaagattgtc taaaagccgg tggcgtgatg aaacgcttag
    22381 acctggcaat caatgatcga gttggcctgt tagatattcc tgatttaacg aaaaaatgtc
    22441 agaaggaaga atgtatctcc ttgtttcgta ccttcaaaag ctatcgttcc ggggaactgt
    22501 tgaaacctga tgataaagat ggtatgggca atactttgta tattggcagt cttaagagtg
    22561 aggtctattt ctgtttgtac gaaaaagact atgagcaata tatcaagtta gggattcctt
    22621 tagaccagac cgaaacgaaa aatcgttttg aaatccgact gaaaaatgat cgagcttatc
    22681 atgcaattca agatttgtta aaaggtcgca gtattgaaag taccacattt tccattatta
    22741 accggtactt gcgatttgcg gataaggtgg aaggcaaaag gcgaactaac tggcccttga
    22801 atgaacaatg ggggcgcttc attggacgga atcagaagga aattcaatta acttctgaac
    22861 cgaagcctta taccattgaa cgaacgttga actggcttgg acgacaagtc gcccctacgt
    22921 ggaagatggc caaggagttg gatcggttga atcagacaac gtacattcag gatatggtaa
    22981 ataatactcg actatcagac cggcacaaaa agattttgga gcaacaaagt atggcaattg
    23041 aaaatttgat tatatgaaaa gaggaatcgt gatggaaatt gtgaaagata ttttattggt
    23101 gtttggtggc agtatgcttg gtgttactgt gatgtgcttg atgcatgcaa gtgcagcggc
    23161 ggaccgagca atggaaaaga aaggaaagtg atgaaatgaa tttcggtcaa aattt
//
LOCUS       DAYYCQ010000042        23147 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000042 DAYYCQ010000000
VERSION     DAYYCQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.042"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..229)
                     /locus_tag="ACHAYF_001626"
     CDS             complement(<1..229)
                     /locus_tag="ACHAYF_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579068.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNE"
     gene            complement(236..784)
                     /locus_tag="ACHAYF_001627"
     CDS             complement(236..784)
                     /locus_tag="ACHAYF_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8579069.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(829..1161)
                     /locus_tag="ACHAYF_001628"
     CDS             complement(829..1161)
                     /locus_tag="ACHAYF_001628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579070.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1175..1432)
                     /locus_tag="ACHAYF_001629"
     CDS             complement(1175..1432)
                     /locus_tag="ACHAYF_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579071.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1627..2169)
                     /locus_tag="ACHAYF_001630"
     CDS             complement(1627..2169)
                     /locus_tag="ACHAYF_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8579072.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(2190..3281)
                     /locus_tag="ACHAYF_001631"
     CDS             complement(2190..3281)
                     /locus_tag="ACHAYF_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8579073.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3289..5499)
                     /locus_tag="ACHAYF_001632"
     CDS             complement(3289..5499)
                     /locus_tag="ACHAYF_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8579074.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5515..8043)
                     /locus_tag="ACHAYF_001633"
     CDS             complement(5515..8043)
                     /locus_tag="ACHAYF_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8579075.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNYQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8095..8505)
                     /locus_tag="ACHAYF_001634"
     CDS             complement(8095..8505)
                     /locus_tag="ACHAYF_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8579076.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8518..8736)
                     /locus_tag="ACHAYF_001635"
     CDS             complement(8518..8736)
                     /locus_tag="ACHAYF_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8579077.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8733..9725)
                     /locus_tag="ACHAYF_001636"
     CDS             complement(8733..9725)
                     /locus_tag="ACHAYF_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8579078.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9804..10715)
                     /locus_tag="ACHAYF_001637"
     CDS             complement(9804..10715)
                     /locus_tag="ACHAYF_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8579079.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10734..11078)
                     /locus_tag="ACHAYF_001638"
     CDS             complement(10734..11078)
                     /locus_tag="ACHAYF_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579080.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11098..11376)
                     /locus_tag="ACHAYF_001639"
     CDS             complement(11098..11376)
                     /locus_tag="ACHAYF_001639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579081.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11735..12235)
                     /locus_tag="ACHAYF_001640"
     CDS             complement(11735..12235)
                     /locus_tag="ACHAYF_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579082.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12236..12781)
                     /locus_tag="ACHAYF_001641"
     CDS             complement(12236..12781)
                     /locus_tag="ACHAYF_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579083.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12778..14034)
                     /locus_tag="ACHAYF_001642"
     CDS             complement(12778..14034)
                     /locus_tag="ACHAYF_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8579084.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14394..14705)
                     /locus_tag="ACHAYF_001643"
     CDS             complement(14394..14705)
                     /locus_tag="ACHAYF_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579085.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14728..16203)
                     /locus_tag="ACHAYF_001644"
     CDS             complement(14728..16203)
                     /locus_tag="ACHAYF_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8579086.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16652..17104)
                     /locus_tag="ACHAYF_001645"
     CDS             complement(16652..17104)
                     /locus_tag="ACHAYF_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579087.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17106..17471)
                     /locus_tag="ACHAYF_001646"
     CDS             complement(17106..17471)
                     /locus_tag="ACHAYF_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579088.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17570..21853)
                     /locus_tag="ACHAYF_001647"
     CDS             complement(17570..21853)
                     /locus_tag="ACHAYF_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8579089.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22000..22239)
                     /locus_tag="ACHAYF_001648"
     CDS             complement(22000..22239)
                     /locus_tag="ACHAYF_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579090.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22329..22571)
                     /locus_tag="ACHAYF_001649"
                     /pseudo
     CDS             complement(22329..22571)
                     /locus_tag="ACHAYF_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22684..22920)
                     /locus_tag="ACHAYF_001650"
     CDS             complement(22684..22920)
                     /locus_tag="ACHAYF_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579091.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6806 a         5009 c         3398 g         7934 t
ORIGIN      
        1 cttcattttt acttgttatt ttttcattga ctataactag ttcatctggt aaaaaaatta
       61 accaacaaac atcgacaaac agtaaatgat ataatccatt tccacctcca agcatattca
      121 atactggtga acatgcctta gaaacacgaa tatagttatg gtttaaatag atattttctt
      181 taaacattgc ttgtttaaaa gccatatgct cttgttctaa acttttcata aacttttact
      241 ctaatgcaca gctagcagca gctgctgcaa ttcctgctac aatatttacc ttagctaact
      301 ctttagctgc agcttttcct aattgttttt ctgcaatttt taataataaa tccgcagctt
      361 tttcaaaatt aaaagctttt aatgcttcaa taaaatctcc ctgcgtacct atagcccaag
      421 ccactcctcc tccaggaaga gaattaatgg ctacacatcc agcatatcgt ttccaatcaa
      481 ctttctccat tctaggaaca ataatatgtt cataaaagac tcttgctgct cttccttgtt
      541 cgttatcttg actgattcgt tcacgaatag caccaaaatc ctttatatgg tatacaccat
      601 gttcgtctaa aaaaccaact gtatcaaaat aaaaagttaa ttcatctact aatttattct
      661 ttatttctaa atcaactgta ttcacttttt ctgtggcata cacacttgtt ccagtactaa
      721 caaaagtact taaacctaat aagaaaacac ctaaaacacc aaatatttta attattcttt
      781 tcataatttc tcctccataa aaaataagtt actttttaaa tatcttgttt atgaattcat
      841 tccccaaagg ccttttaaga ttgatacagc agtccgttca cttcttttag acttgtcata
      901 caagtctttt aaatgatccc atatcagact agtttgtcta tctagcttca atccattaaa
      961 cgctaatgaa cggttcattt tttctattac tttagataaa gctacagaaa tttttatacc
     1021 ttcacaacgc gcatcttgga tttcaataat agtatcatca attagttttt gaatttcttg
     1081 cctatcaaca tattcaatct tagaaagaga atcttttaaa gctactaatg attcaacgat
     1141 ttcttttttc tcttcagaca ttttcttcct ccttctagtg acccgtaaag ccatgtagag
     1201 cagagtccat tgaaccagta attacactat ctacaacgcc attccaattt actctacatt
     1261 ttccattttt cagatttgtc ggtccatacg gatagccttt ttgacattca atgttcggat
     1321 tataccagat aggattatac cattcagccg cagaagcaga ttcagtcgat acaaacaatc
     1381 ccacaccaac aaatccaata cacatcacac tatagccaat tagttttttc atctcgtatc
     1441 catctcctaa aaaatagaat agttcttata gttattcata agacatattt tttccgtttt
     1501 tgttttatcc tttctgtcct ataacaactt ttttcaagta aagaatgaca tagtttttct
     1561 taaaaaaaca gtctgttttt ttctatttct tcggtgtata ataaatatga gtgttcattt
     1621 tttattttaa tacgcactcc gtacataata aatcttgatc gcatcaatct tttgagcttc
     1681 ttgacctact ttcaattgaa gtaatacaaa ttgagtaacc ttattcattg tttcttctcc
     1741 aaataacaaa tattgatccg gattctctat atcttgtgtc acctggtaaa gtttcccagt
     1801 tgttttcatt tgtacatgcg ggtctacatc aatactattg tctttaatac aacgatctgt
     1861 catatagtcc ctcacagctt gattgcgttt ataaacatct gtgaaattac tccattttct
     1921 agcaaaatct tttaagaaat tttttgtcat ttcatctatt tgcatttgtt gcgctgtctc
     1981 gtcttctaat gaaccaattt gactgttact ttcagtactt tgaagagttc cacttgtttc
     2041 ttttactgct tcgattgttt cttctgtcgt tctcaattgt gttttttgtt tttcagaaga
     2101 aaaccaatga caaccagtaa gacctatcaa acataaccaa ctaaatacta aaattatccc
     2161 ttttttcacg tttctagtca ctgcctttct tatttcacct tacgaaccag actacgaatt
     2221 tttgtttgat tgtacgtgcg ttgataccga gccataatcc gtccttgctc ggcattctgt
     2281 tccacactag agaaatgttg tccgccatat gaaacatctg ttataattcc ggtgtgacca
     2341 tagattccag gagacaatgc accacctgct tgccaattca ccacatctcc tgcttgtaaa
     2401 tcacttggtt ttgggttcat gattacttgc caaccatagg ttgtccagtt ataatcttcc
     2461 cctattaaca tcgcaaacat gtgtccagaa cccattaact gtgggccacc taattgagat
     2521 acataccatg ccgtaccacc ataacattcc ccattattta cggatcgtcc aatgatccga
     2581 tccaaaacct tcgcattgcc tttacttttt cctgcaataa gcgaagtttt ttttgttggt
     2641 aggctattcc aatgcttgat cgcaaaagta tcatattccg ttaagttata ggacgttatg
     2701 atactcattg ctcctcgata atacggactc ccttcggtaa tacctgctgc cgcatagcct
     2761 ccacgaccta gaatcaataa acaacttcca ggatctttat tgcccacaca ttgattaaag
     2821 ttgctttggc tcgccaccat aaagccataa tagtcataac aagcttttaa gttcggaaac
     2881 ttcatataga aaccaccttc agaaccacca attccacgtg cagaaccttg tgggtaggga
     2941 caaccaggaa accaggtaat cccaaaaaag ttcgtatcgt ttcttccaga ttgtgactgt
     3001 ccataagctg attcatagcc taattgagaa agaatcacac tcggtaataa ctgatacttt
     3061 acagctgcac caactgctgc ttgcgcatgt tcatctgtga ttggaccacg tcctttaatc
     3121 tctgaaaaaa tcggtaatcc ttcggtatat tccccattaa aagcgacagc tagacctgta
     3181 gcttctcctc catgctcaga aagttctcct gaaaaaaaga atggcaatag aaaaaaggga
     3241 agaataaaga aaagaagaca acctagacac cctttcttca tttcttcctc atcctttctt
     3301 cgataatggt ttttggactg ttcgtccttt tggtaaagta aaatcagttc ctggttttgc
     3361 gtccaaaatc ggcagtgttt taggatcctt ttctattttt tccgattgct ctgttggctt
     3421 atctaaagaa gaaaatgcag gttgtaacga ttttggtgcg gaaatcgacg gtttactttc
     3481 agacgaacgt tgccccggtt gttgcacaga acgatttcct gatagagtgg ctaccgacga
     3541 attaggaaca gagtattggt tcaataactg ctgcattgct ggataactcc catttttcgc
     3601 ctgatttttc acttggcgaa tctgttgtga aagagaacca gcagctgtta attgtggcac
     3661 gggtactttt ccagaaggca acggaatcga ttgaccaaca aaagcgatac ctttatttaa
     3721 caatggtttt ctttgattat tcccttgttg cttactcaat tgtgccaatg attgtacctg
     3781 ttgttttggt cttactgaat tcatggacga atttggaatc aaaccatttg aaagaccaga
     3841 attccactct gttttcgatt tggtagaaga catttcaggc tctaccccat gataacgaac
     3901 atgacgacca ctataaggca aggcttgatt tgaacgccca cctagttcaa agggattaac
     3961 cacttgcctc ccttttaccg attcttgtgg tgtacgtttt tgttgaaact tttgaaatcc
     4021 atgtatcgct gcagaagcga gtggattcgt tgctcgatac acattccctg ctaatcctgc
     4081 acctttgatt gccaaatttt gtccagcagt tgatagttga ccaggcagtt gtttcatttg
     4141 agacaaagaa tgacttgcta tgtttttcgt gttgtttata gtttgatctg cagctcctaa
     4201 ccctagtttc tttaataaat ctttccgttt tacaatgagt aaaattgata ggagtgcttt
     4261 gacaaacata tttcctaaaa aaccaaaaac agtattcatt ggaattaaat cgtctactat
     4321 gttatagatc aaaaatgcca ccaaaataat cacactgtaa aaagattttt gaaacatcaa
     4381 gcccaaaaag gactttaagg tattcatgag catataatca aatactggaa caaaagaagc
     4441 gagtaatgaa aaaggaatca caagtaataa acacaacacc aacatttgaa agataaaacg
     4501 aatcatgcca atcactacaa gtggtaatcc aatacttaga ttaagcaatg gcaccataga
     4561 accaacgact gctttataaa cagctttagt tggttgaata tagacacgat aagccttctt
     4621 tttccctcta tcttctcctg cagcagtggt cacttttgtc tcaatatcag ataattcttc
     4681 acttgacgct tccttggcta gaaattcttg tggttcaatc tctgcttgat acaattcttc
     4741 taaatttgtt gtcccataat ttaacaatat atacggatca acaacagccg ctttataata
     4801 aagttggcgc actttttgaa tggcttcctt ttgtgtcgtt ggcactaaaa caccattgac
     4861 atcttttgtc gccaattcat ctagactatt tgtagcagaa aaaataaagc cttctacctc
     4921 cacggacaaa tcatttaacc ctttggtaaa agcttctcct ttactaggta cgttactacc
     4981 atagccaaac caaaccattg aaaaaatgac aacgaagaga aatcgaataa acaccatttt
     5041 tgcgtaatgt ttccctctaa atatacgaag agtaaataga tataacacat acaagacgac
     5101 aatccccgca ccaataggat cgtatatttt tgaataaata gtatccgaaa aagtgaaaaa
     5161 ctggtgaatt aactcgttca ttgcagctcc ttcatagagc ttctcaatga caaaatcaag
     5221 gccttgccaa atcaatttac ttaaactaaa gaacatattc gtcatcattt ttagaaaatc
     5281 tgtgccattt gccccaaata acattttgcc taatgaaaat tcactatcag ataaataaga
     5341 agaaaaggta tcaatatgaa atttcccaat ctcatagatc gattgatcca cctgcttttc
     5401 tgccgcatac accaatccac caacaaaaag cagtccgaat ccaatcgtta aaaaaaacaa
     5461 aacgcaccat ttcttttcct tatgctttat ataagccatt gtttcacctc atttctattg
     5521 catgattaac gtatcctgtt cttctacttg agagtgactt gttttttccg ttgtttgtaa
     5581 tgcagcggtc atttcttcaa acaaactgtg tacattgagt tttcctacac gtccataggg
     5641 atctaacatc caacactgtc ctttaatcat gtggctcatt tcagctaagt tcgcttcatt
     5701 tacttccaaa cgtaaatgat tcaaaatctt ttctcgctca ctgtcttcat caaaagcaaa
     5761 gacgacacca aaattccctt tttctttatc tgaacctaag tcaccaataa attgtgtatc
     5821 taacactaat tgattcatct gggaacgccc cacacgtgcc attgctgcaa taatactacg
     5881 tcctgcgttc gaattattaa atacccatgc ttctgtaaaa taaatcgctg ttttcttact
     5941 actatctcgt tttccaaaca tttcacaaaa acgacctaaa ctaatcatca aacacaaaga
     6001 ctttcgatcc gcatctgtat atagttctgg tcggacattg tcatttggta atttcaaatc
     6061 ttggatctct aaaatcgttg ttttggcatt aaagtctaat cccgggtttt ctccataaga
     6121 aaatcctaaa cgtaaaatcg aatcttctac cgtcaagcgt aaaaactcac catattctcg
     6181 aacctctttt tgttccattt gttctaaata gtcgaccaca tgcatcaagc caacttgttg
     6241 tccggcttct ttttgaacta aaacttcttg gattcccttt aataccgccc cttttaaaag
     6301 gtgattggta cctaacggac ttaactcatc aatcatatcc tgggctactt gtttcgcatc
     6361 tactcgggat aaacaaacaa ttggatctaa tacaccataa ttacggggat ttgtagcatc
     6421 taacgtgaca taactcattt tttgcaaatg ttctacaaac aaagggtaat gtttctgata
     6481 atacgggtct tgaatgagtt tcgtaaacca tttgcgcttt tctcctttgg gatccacaaa
     6541 taaactttgt acattcataa acgaactata caaaaagagc aagccaacta aaaaggtctt
     6601 ccctttaccg gtttttccag ttactgctac atgaggcgca tcaaacaacg cacctttgat
     6661 tccttccgca atggctagaa gattcaaaaa gacaaaacga tttgacgcat aaattaattc
     6721 ttctaatggt gttttttctc ctcgagaacc accatcccct aatgtatctt gccgaccaat
     6781 ataccagccg gattccatac ctaattctga tactgctgaa aacaaccatt cgccaactgc
     6841 ttctacagtt gttcggtgct tccaatgtaa ctgccgtccc aatggttgcc ctaataaaca
     6901 tttatagaac aattctcgtt gataattttt tgcacggtgg atatggataa tcgtatgaag
     6961 ttgttgaata accgttgcac tattttgacg aacttcttca accgtttgac cgtatacaat
     7021 aaaacatgcg atccaatcga cgacaggttg cccattatct actcgttgct ttaaatgttt
     7081 tactaatcct cgaacagtta ataagcgttc actaggttct tcatcatttt gaaccaattc
     7141 cttatgctct gttttgaacc gttccgatag ccaattcagt tttaaaccaa tacctccctg
     7201 tcctttcaag cgtggatagt gaattttcat tcgcaattct gctgggaaag aaagatcctg
     7261 agcaaattga aatagatcac aatttctcat attttcaggt gtttccccaa taggcaacaa
     7321 tgctaaataa ctactcccta actcatcttc taattgaaga aggcctttat gttccttcgg
     7381 tatacgtaca tacgttcctt gattgtcttt cagtacttcc caattatttt gttcttctac
     7441 tttgtgagcc attcctcgaa tatattgcag acgattgaga tacaacagct ctttttttac
     7501 taagggacga atagaaaagc tataaagtaa ttgtgtcact tcttttacat aaggatcact
     7561 ttcagaaaac aaagtttcca aatcatctac ttctaatcca aataagcgtg ctaaacttcc
     7621 tgctaaatct aaccagatta gatttattac atcatgaaaa ctctccaccg ttgctttttc
     7681 tttgactttg attcctaaaa taaatgaagg acgatagact tgaccttctc gttccaacaa
     7741 tgtcactgtt tcttttgcat aatacgcacc aattgcttga ctatcgggag aaaatccctt
     7801 tcctaatgtg tcaaacttcg tccgtaaatt cattgttcgt ggatacatca ccaactccac
     7861 atcttcataa tctgctaaaa gcaacagtaa acgagccatc atttctttta tttcctgtgt
     7921 cttttttggt gtgttttttg aaattgttcg tggaaaaaca tgatagtagg cccagataca
     7981 atcctctcca tttaacaaac aatttttctt gaatgctttt actggttctt ttacaagcac
     8041 caatgaattt cacctcccaa taaaaaagga acaaccagtt gattgtccct ttttctaatc
     8101 ctctaaataa acatacttat tcacaaaaag taattcttcc aatgcattgt ttgatacctt
     8161 gtcccagtta taatacacac aatcaccatt catcatacga atcatataac aaaaccaatc
     8221 ccataaataa aaatgcactt tttttccatc aggttttagt tttgccagtg ctaacacaca
     8281 taaatagggg agtcctatat aaaaaacaac tcctgctggt ggaaaaaaag aaatgaccca
     8341 accaataaat gaacgtaaaa ataaatattc aaagattacg accaacaaaa aagttgctgc
     8401 attgactaat gcaactggaa atggaagacg ccaattgctt gtaatctgtt gtacgcgata
     8461 aggttccgca taaacaatcg aataatcata aggttcccaa tccacttcac tatcctccta
     8521 atccataaat aatttttcaa tgattttacc aaacgcatca aatacacgtg ttggatcatt
     8581 taagaagaaa taaactgcgg cacctgctgc gatagatatt aatgcatttc ctacttttga
     8641 tttcataaaa tttcgcaatc ccagataaac aaccaccatt attaaaaaat agccaccttg
     8701 cgttaatata aaatctacaa ttcccttcag tgtcatttcc gttcttctcc tttcctatcc
     8761 aaccaattat gtgttaattt ttcaacataa tactgatttg tttttttcgt aatgacaatc
     8821 gtcatttgtt ctttttgtaa aagtgacgta tcaccatctc gaaagacgac cgttgctttt
     8881 acaataaaag gttttttcgt ttctttataa cgcaatactt tcatctctac ttgatcaact
     8941 ttagctgttt gattgagtcc ttcaggttct ttcatcatgt atgacatatc ttttgcattg
     9001 tttttcgcat acttatctaa gaaatcttcc acaaatcctt caacatcttt cgcttccgac
     9061 gcatcaaccg tttcgtataa tgtgtcatca taagtaatca ttcgttgttt ttctgtattc
     9121 tttggtagtg ccgtaaaata aggataagca ataatacgac agcccttgtt tgaaatagaa
     9181 aagggaagat tgagtacttg tgtgattgtc acacttgttt ctttcttact ctctggattc
     9241 atcgagcgaa tatcataagt tactttataa ctagcagttg gaatggtttc aatttctttt
     9301 aatccaataa acgaagcagc agttaatttt cgaacacctg aaacacttcc tggttcttga
     9361 ataaagtttt gtgcataaaa ctcttttttt aatgtttctt gacgttgttg ttcttgttca
     9421 tgattatcga atgtattcat atacaaagca acaaaacgat tcataaagga ttcaatttca
     9481 ggtgtgtacg ttaattctga ccgactttgt acttctttct tttgtaattg ttggatttca
     9541 tctttcaaaa acgacacttg aaccttcgtt ttcatggatt gaaaaaatgc caagccccct
     9601 gaaaaacaaa gaaataaaac caaacaccaa atccatttca ctgctttttt tgaatggatt
     9661 ttcttttgct tcggtacttt ctctgtataa actttttctt tccgccgtag ttgtcgtttt
     9721 ttcataagct actccttcta gtatgttttt actagtacgt acgattgaca acgataagct
     9781 tgtttttctg aaaaatagtc cattcaatta ccctcatttt ctattccttt tctctttctt
     9841 ttatatccaa caaatccagc aatacttgtc aacactcctc ctagaacaga aagtgcaaca
     9901 tgtatttctt caccagtctt tggtaagact tttgtttttc catctgctgt tttgatttca
     9961 atattgccac ttggaagttt ctttacagga atcgacaaat cctttaccaa attcccttca
    10021 ctatcttgga ttaatggaat accatctttt acaccaacaa ttgtttgatc acctgctact
    10081 tttaatgggg tggttggtgt tggaatatgg gtcaattcct ctacattact agtgacaatc
    10141 ggtacactca ttccttgaga agtattcgtc gtaatttcac cgcttggggt taccgatact
    10201 gtgacttctt ttgctggaac aataggttta ttttcttttg gaattgcttc tgatttttca
    10261 acaggttgtt tcggttcttc tttttgttct tcactggtag acgacatcgt tggctgttcc
    10321 gtacttgacg aatcagtatt cttttcagaa gaactggttg ttcctgattg ttccggagtt
    10381 gtgactatac ttgaatccgt tgttgttgaa ctatgcacac cttcttcttc gatcgaatca
    10441 gtcggtaaag ttgattcagg aaactctggt tctggtgtca ctggctcttt ttcaggtttc
    10501 tctgactgca attgttcagg ttctgctggt ttttccgttg ttcctccatt tagctctgtc
    10561 tcaacatctg gtaatccttc ccctggcaat gtttctacaa tttctgactt aggtgtttct
    10621 acagtttcat ctgcataaac caatgaactc cctaattgaa acgaaaataa tgaaattcct
    10681 agtgttgtta cgactgctaa tgttgttttt ttcatgatta ttcctccaat tttttatgca
    10741 attcgtgttt tgttcataga cgccgcacta atattatctg aacgataaaa gaactcccgt
    10801 ccgttttctt ctaacccaac atatggatta tttttgaata attgaatcaa tagttcactg
    10861 gtgttgtatt ttggatagtt tgcaaaaaaa taatactgtg cttgtcgccc atccgaaaat
    10921 tcaacaagta attcataacg ataccgcttc aatccttttc gttgttttaa aggatctcta
    10981 taccaacttg tccatcgttc cattcgtgga tagagataga cacgatacac atagaacaat
    11041 gccataccac ataccaccaa aaataataat cgccacataa aaattccccc ttttcttcta
    11101 cctttcttga atcatcgaac attcataccc tctatagaga tttgaaaaat ccatcatcaa
    11161 ttgttcatat aaacgttgtt gcaaatagcg attgtttcga atacataatt cttcatagcc
    11221 atcaatcact tgatcttctt tttcatagca tcttgtagct ctatgacgct cagcttgcaa
    11281 gacataacca aactctgcac catatcgttc tactgttaat aactgttgac tacgcaataa
    11341 tgcaatataa caatcacaaa aatgttcaac cgacatttga tttttctcac gtttgtgaaa
    11401 taggtattct ttagaaagca cattattcgt taaacaatct gataaatagc gataatctgg
    11461 cggtcggtta ttttcatctg tgaatttttc ccataataaa ctgattttta ttcgttcagc
    11521 ggctagatag tcttgttcgc gtggataaaa ttcatcaatc gcacaaagtt ttttatcacc
    11581 tgaataaaca aatccatata atgcgaaacg aactcttcct tttcaattgg ctctcctaaa
    11641 aattccagtg acataccatc ttctccttcc taaaaaaaat aaaaaagcag aaaccgattt
    11701 ggcttctact tttctttcct tttatagctc ttttttacgg aagaatccgc aaatgacatt
    11761 ttttcaaaaa atcaatcggc acaagttcac gaaaacttgg agcaattttg gatagaatca
    11821 cttccccatc ttgtaaaata caatcataaa aatcatcagt tacttgatac ttacgcttct
    11881 tacaataatt gataaaatag tagcgatcat tttctatttc cattcgttgt tctactaagc
    11941 gcatacgaac atcatcatag aaataagcca caaatcgccc ttcaagttca tgtttttcaa
    12001 tgggatactc ttctggtaac aatctatttt ttcgtcgttt atacattgct ggatacctta
    12061 gaagaagttc atctaaacgt ctgtcagtat atggtaactg acgaatcaac gattctaatc
    12121 gttctttata cagatagaat aattggcgca agcaatagtg taccgcctct ggattttcta
    12181 atactgttgg atcacgtgac caatacgcac caacataaat cataattttt ttcatttaga
    12241 caatcacacc agcaatacta atgaccgatc cacaataggt agaacaagtg gcatagcgac
    12301 caatttcctc taattctatc tcaatctctt ctaaagaaag atatgtctgt tcttctgttg
    12361 gctggcgact catttcccaa gtgacagtca ccaatgaaat ttcttccata agaggttttt
    12421 cgctgctaag tgctgccaca cagttttcat atatcttcat tttatcgcag ccttttttaa
    12481 ccgctaaaat cgcaggtatt ggcaatgaaa atcgataaaa gcgaatgaat tctagccttc
    12541 ctttccttcg gctaaattcc aactcttcga gttcttgttc gtatccctta cgaacttcta
    12601 ataagcgtgc aatttcttgc cagttctctt tttcctgata acgtccagct tttaccgctt
    12661 gttcaatata aggaactcga aaacgaaaat aagacaaatc aaccatcttt tcatttaatg
    12721 cttcgattgc agcctttaaa tccaacaggc gcagccattc tgttgtcgta tatgcattca
    12781 tgcaattttc cctcctgacg ttcccatcca ttctagatag ttttgttgct cgtcatttag
    12841 ttcggcatta tcaatccctt cttgtacaaa gtctcttcct aaaatacgat ctgcttcttg
    12901 taacaacttt aatgtcggag ctacttgttt caaaaaccag cgttctgaac gatctaatga
    12961 gaatttttct ggctttactg taaaatccaa ttcttccatc gaacgaaata attttgccca
    13021 tcgtttatca acaatccgtg catcactttc cagtctatcc caaacaataa gttttgcatt
    13081 aattaaacca acgccaatct ctcctaaacg tttcggtgaa tcacctaaaa taaatgcttc
    13141 tactgcttgt gttgctttct cattggctaa ccgtaactca tatcgattat aaataccata
    13201 tttcgttaat gcttctaatt ctgaaatttt ttctcggttc gcaatttcaa aacgtttctc
    13261 ataaaaatta aaaaagagag gactctttcg cgatccaaaa tacaaagata atccttcact
    13321 tcgaaaatag ccatcatcta aaaaacaacc gccaatattc tggaatctct taaagtccat
    13381 ttctaataaa cctgcttttt gtttttctag taattcaaag agatcgaaat gttcttccgt
    13441 ttccaatccc caaagttcat ctaacgcaat atcgaatcgg ctacattgca cacgtgtata
    13501 accagcttcc aactttcctt gatttttcca aatggaatat agccatgttc gccaatctgt
    13561 ttgttgttca cgcatgactt cctcatattc ccggcaacct tgtcctgaaa attgcacaag
    13621 tacattttct gttacagttc gttttgaatg ccagaacacc cagatattcc cacgaacaga
    13681 aagacagtca aaaagataca agcccgttgg acgtgtttca aattctgtca gatccattcg
    13741 taatacacgt tgaatcactg ttttgtagtc tgtcccaaaa aaggaaagac gcaaataatc
    13801 aatcattgct cgaagatcta ttccgctttt catatacaat cgaaagcgtt ccttgattcg
    13861 ttgttcaaaa tcctctggga tttttctttt cccttgttcc atctttctaa tgtaatcttt
    13921 tgagcagccc atcaattgcg caaatcttgc ttgtgatagc ggctttttat ctccacttat
    13981 attatgaatt gcacttaact tctttctgta gctttttagc tcaatcggtg tcattttctc
    14041 gctcctttta taaaataaca aaatcgattt ttttaaccct ttagaaacac cgatatatca
    14101 actagtcccc ctcgtttttt gggaaaagta gtacaactgc tgaccttaga taatcacttg
    14161 atacaaaagg gtttataaaa cttttttcta tgtgttatgt tatttctgcc ccccatatta
    14221 tatatcgggg ggctttacac aaagaggccg tgcgcaagcg cacgacctct ttgtgagctg
    14281 cacacgctgc tagcgagtgc gcattatagg cccatgcttc gcagccttaa tgcgcaccgc
    14341 caggcagagt gcggctacca gactacgtct agagccctcc cgatagataa taactacaaa
    14401 attaaatgat ctaattttgt tatttcacta tatttttcag gagaaaaaac gcgataaatt
    14461 gacaaagtta aatcctcttc taaatctact cgataatgat acaatcgagt caatcgttct
    14521 atatcttttt tcgttagcaa tacttgatta ataacttttc ctgtaaaaac acgttctttg
    14581 cgcaaaaagc ggtaatcagt tacttgttca atacgattca accaatcata aaacgttctg
    14641 gaagaaagag aaggattaat cattcgtatt gctgtagcta ctctataata acaactctct
    14701 gccatttaac ttcacctcct tagttgatta caaaatatgc gcatcagaaa aacttgttcc
    14761 ttttggatct tttgctaaat acatttggcg ctctagttgt tcttgtaaca actgattatc
    14821 atctcccatc gtttgatctt gactatcctc ttttactgta aaatcataga caatcatttc
    14881 agtaaatttc tcaaattctt tgataaagtg ataatctttg taaacaaatg gcgcaaaaaa
    14941 ctctacaggt acatcaaatc ctggaaaagc atatccacga ccaggttgat ctttgttgta
    15001 aaatggctta tttttaaaat cttctccaaa agtcattaaa taaccaatgg gagacaatcg
    15061 accaacagaa atacggaatg ttaaattatc ccgaacacca cttggaaaat attctgcatc
    15121 tggtcgttgc attgctaaaa taatgtacat ccctgcttga cgacccttta atactaatgg
    15181 ctgaatcaat ttatcgactc tatttctcgc ttccatatcc aacgaaccat aaaaagctgc
    15241 ccattcatca aaaaagataa aatgcggtgg taacccataa taagcataat ttttccctgg
    15301 ttcatattta ggatctttca tggttttcat ataggcatac cgttgattca tttcttttaa
    15361 tgcatttcgt aaacaaataa tcatttcttc atttattttg taaaacacat gtcctttgaa
    15421 tacatcgacg tcacctaatg ctgctaaatc tgccgctttt ccatcgcaaa tatctactgt
    15481 cccaacactt aaacaagctt gaattaaaga atacaataaa taggttttcc caccaccgat
    15541 tgcaccagca atcaacatat ttggtgattg atcatatacc caatccaaag ttttcattaa
    15601 atgaatacgt ccattttctg ctatacattg atcaatagga atccgaccag aatctgaaag
    15661 gaatacataa ttgattcggg ctttttctcg ttcttcacga attaaatcac gcaaaaatac
    15721 ttcagataac aaatgaccca tttttaaaaa tcgttcgtga aaacgcccac catctaaagg
    15781 aaaagaaacc actaattctt gtttcttttg ataaaaataa acttttggta atctcatttt
    15841 ttcccttgtt tgtttctcac taacttgttt ctttgtataa acaaaccgat tgtcaattaa
    15901 cattcgaaca agtaactgac gccaataaac agaggcaaaa tatccttctt ttagtcgcga
    15961 ccaatgcatc aaacctaaaa ttaacccact aaccagcaac aaatacaaca caatacttat
    16021 tccaggaata agatacattt gccaattcga aaacgtactt aaacgtggat aaaatacttt
    16081 ccaactaaac caacctaaca gaatcaaaaa tggaaacaaa aagaaaccat aatagcaaaa
    16141 ccataaataa cgattgaaat agcgaattcg ccgaccacga tacttgaaaa aatgagcttt
    16201 caattgtcac attcctcctt tctgttttta cttttcctgt gctaaaaact tttgacaaaa
    16261 agcaactaat acaggaatat cacatttagt aaaaaaacat aatcgaaaaa gatttaatcg
    16321 atttttataa gggtgtgcac acatattaca tattcgtact atacgaatcc aaatcacttc
    16381 ctgatgatct tgtttccact ggtacggtaa tgaaaaacga acaagatcac gtaaaaacat
    16441 acactgccca ataaccgcac aaatatatgc atttgtttgt acaaaaatcg ttggattgtc
    16501 tccaaaatac tcaatcaatt gttggatatt ttgataacaa gcacaatctc tcaaagtatt
    16561 tgattggttt tatctgaatt caaaatctcc ccttccttct atcttagata aaaagaatag
    16621 gaagactcct tcctattctt tcaaaaaaac cttatttttt ctctttttcc ttagaatcaa
    16681 cacttggttg tttttgatct aaaccttttt cttgtgattg tttcatatta ggattatttg
    16741 ttggagcttc tactttctct aatcctttcg cataaatcac taaatctgaa tattcacgat
    16801 tgccatcttg tgctggaata tataccacat gatattctgg atcaattaat ttcacaggat
    16861 cacgtcgttt aaaaccagtc aaatctacat ctgctggaaa tttaactcgt cgtgcaatct
    16921 ttggttcatc tggatttcca aaaactaatt gtgctcgctg tgatcgaatt tctcccgtat
    16981 aatcgcctgt ttcattatca aatacacgat cagactcttc taatgcgcca aagcgtacgg
    17041 ctcctaaaca agtttcatct aatgcttctg taatgtcata attatttgga aattttaatc
    17101 ccatcttaca aaacctcccc aatttttaca ccagttaaaa agacaccaac atcttgatag
    17161 ccagttgttc gacgctctgt ttgtcccata tcatttgttt cacgactatt cactcgttct
    17221 ttacgaaaaa caaaataagc ttcttcaaat gaaatacgca cctctttttc tggtaatcgt
    17281 aatcttttat cctcttcagc tgtgagataa acaaaaaaag caggctgttc ttctcgcgaa
    17341 cactctgctt gtaattgaac atatcgccca gacacaccat taatttcctt tggtggatta
    17401 ggttcccgtg aacccaataa aaatacttga cctaaaaact ctttcttcat acgcaatgct
    17461 ttttctgcca taaagttttt cctcctctta aaaaccaaaa taaattctaa atatttctac
    17521 attacaatca aacaagcggt taaaaatcct aaccgcttgt tcctcatgac taatctttgg
    17581 ctttacgtac tttataaagt gttataccta aagcaatgac taatactact aatccaagaa
    17641 ccataagtat actacttgtt tgttcacctg tttttggcaa cattggtaca acagtagctg
    17701 gtttaacccg ttgttctggt ttcactggtt gcggtggttt tggcgcttcc acaatttgaa
    17761 ccgtttgatc tttgtttgtt agatctacat tgtgtttcgc cacttcttca aacgtttctt
    17821 tctcatcttc cgttttcgct tggagccatt ccgcaaaact caagcctttt cctgcaagat
    17881 tgcgaccgtc cactaagaat tcaaataaga attccatcat ttctttatcg gccgcaaaga
    17941 ctttttccgc atgagtgcct tcaaatggtg tgccgtctgg tgttaagtag ccttgaacaa
    18001 ttaagcgata gatttgacca atcgcaagac cgtctacttt gacccattca agcacagttt
    18061 gatttggcaa ggccaagatc tcttttccgc catttttatt cgtcgctttc gtcgtaatgg
    18121 ttggattact gattttgatg gtttggtcat tgtcggtaat atctgcgtga gaagccaata
    18181 atacgccttc tctaaacaga tcttcaaaga ccacgacttc tttgcctcgc aacgcacgtg
    18241 cgtctagaat aaagtctaga ttgactgtac catccgttgt ttctgccaca aacgttaagg
    18301 tacttgtgac ttcttttcca tctactagca ctggtttttc cgttactttg tccattagaa
    18361 caccgttgac tgtgtatgtt tttccaacga ccaaatcgtg gtatttcaca gcgtctgtaa
    18421 tggtgacttt cgttaatgga ttaacctctt tcaagccagt gggtttatgt gtagccgttg
    18481 tacgtacact tgggttagtt actcgtaccg tttgcccttt gtcgttgatg tctttatggt
    18541 ttgctagttc ttcatcattt ctaaaaagac tttcaaacac gacgatttca cgaccattta
    18601 tacctgtcaa atcaaaggta aatggtacga tcaccgtttc attttctgct ttggcaataa
    18661 agccaacact cgtttctacc gtatttccat gcacaagcaa gggttcaccg gttgctttat
    18721 ccattaagat ccctttgacc aagtatgctt ttcctggaag aatctcggta atttcaacgg
    18781 tatcgtttaa ggtaaacaat cctgttgggt ctgcgactgt caatccgtct tcaaagacag
    18841 ccgtggtatg aacggttgga ttattcactt tcacactttg ggcttcatcg ttgatatccg
    18901 catgtgctgc aacttcaatg ccatcacgga aaagtttttc aaaaacaacg atcgtttgac
    18961 ctttcagaac actagcatta accacaaatt caaggtcttt tgtgccattg actttgtctg
    19021 caataaaggc caaggtactt gtgatttctt tgccatcttg taaaattggc gcattcgttg
    19081 ccttgttcat caatactcct gaaatggtgt attcttttcc gacaaacaag ttggtataag
    19141 aaacactgtc tttaatgacg atcgtttcta atggatcaac cactttttca ccatcggctt
    19201 tatttgacgc ttgtgtttct agttttggtg gaatcaagta attacgtaca gaaaccacta
    19261 acgtttcccc atcatctggc gcattttgtt tgaatgcaac cgtgaatgct tgtttatctg
    19321 tatttggttg gaaactttct aatggggcat tttccaagaa gtaatagtca tcggcaacta
    19381 gacctttcac taaaatttcc ccgttttgat cagtggtata ttcaccaatt gcttgatttt
    19441 taccgttata caaggtaaag ttcacacctg cgagcggcgt tttcttcttg ttccattctt
    19501 catacttgaa taattttaca cttttccgtg cttgacggtt cgcaaaatgg atcactttga
    19561 tcggttctgt atcattggta atcgagaaca ctaatgggtt ttgattcaaa acgtaattta
    19621 cgggggcttt gatttcttcc aaacgatagc gatcttttcc ataagtaagt gtaccgttag
    19681 ttaggaaaaa gcctttttca ttggttttaa agatatccgt tgtttctgta tcatttggaa
    19741 tctccattgt gacataacga ttggctaaag tatcaaaaat cttgaattct gtattagcaa
    19801 gtggaatcac ttttcctgtt tcttgatcca ctttcaccac tttaagacgt tgttcaatta
    19861 ctttatcttc taaaacataa tggaaagttt gcccattttc agaaattgtg acttcaagtg
    19921 gttcaaatag tttataccct tcttttcctt tggtttcaga aagaatgtac gtatcaaaga
    19981 caacggtatt tttaccaaaa gtagcataac catcattgtc tgtcacaacg gttttcacca
    20041 ctttgccagt tgtttttgac gtcaaagtga tttcaatgcc agctaatttt ggtttgttat
    20101 ctggattcgt tgaatcgact aaatctttat tagcaacttt cactaaatca atggttcctt
    20161 tgatcacttg atcttttgcg gtcgcattga cagtttttcc aggttcaaca acaatttcaa
    20221 ttgcttcatc agaaagcaca taaccttttg gcgctttgat ttcttttaaa acatatttgc
    20281 caggatcaag atctttaatt tctgcctgta ctttgccgtt aatttttttc aaggtaattt
    20341 ctttaatctt tttcccatct gcttgataca aaccatagac cgcattttct aaagaagctg
    20401 caccttgtgc tttcacacca gtttccgcat cttctttaaa cacacgaatg tttccttgtt
    20461 ttagaatttg taaattaata acagacgcat taggttctgt tggatgtaac acaccaattg
    20521 tttgagctcc tggacgacga taggctaatg gcgcaccttg aactttaatt ttattcaatt
    20581 gaatttttgc tttatccggt gcctcttttg tgactgtaat ccatacttca ttgccgtgaa
    20641 tagatacatt gacacctttt ggtttttgaa tgtctttcac atatttcaaa aaatcatttt
    20701 tattctttac tttcaacgtt tcgccaattt ttaccgtatg tgtttgatta ttaaaagttg
    20761 ctttttcacc aaagttatta atcaaacgtt gaatttctgc ttttcgacga tcataatcaa
    20821 cacggatatt tttgattggg atttttgtgc ccaaagcttc ccaaatcata aattgtgccg
    20881 caatgtattg ttcatcggat ttgtctttat ttgtgacata tcctaaataa gagatcaagc
    20941 ttgttttttt ccgtacattt gaatttttca aatagttttt caaagcagta gctgtataaa
    21001 cttgaccttc tgcggctgag acaccaggct caatacaaaa cgccacttgt ccattaatct
    21061 caaaacgaac catataattt aaagtgaagt ttagattgtt cggactagaa gtaacaccaa
    21121 tattttcgcc ccaatcaatt ttccgaccac ctaaaacagc tgcattggtt gcacggcgaa
    21181 cacgaaccga acgattggtt ggtactgtta ctaacagtgt aggtgtacct gtttttggaa
    21241 tgttctgctc ttttttcttt tgattttcaa tactagccgt agattctttg tttggtttcg
    21301 ttggttgatt ttgactagct gcggttgaag agattgtttt ttcaacgttt tcctcatttt
    21361 ttccttcttt gtcagcagta gccgctggtt gttccactgt ttcttctatt cgtttctttt
    21421 cttccgcttg tttctcagaa gactctggct gcacagaagg cgttgcttcg gcagcttttt
    21481 ctccttctag agtggacgtt tctgaagcag gggttgtaat tgaggcagtt tcttcaactg
    21541 gttgtgaagt accaggacta ttccctgggt gttgtgtttc cccaatcgtc tgatcgagag
    21601 tcgttttgga attgaccgtt ggcgcttctg tcacaggggc ctgttcaaca gtggcttcac
    21661 cagctgggac cccggcttct gctggttgga cttctgtcac ttcagaagcg acttgggcaa
    21721 tggcttccat cgtaggttgt atagccgtcg tttcttctgc atgtgcttga gcagttccca
    21781 ccaagaaaca gaattgagaa aaagccacca atgcgagtgc tttcaaccaa ttcattttcg
    21841 tttttgcata cacaagaatt cctccttttc ctgagaattt tatataataa aacaaaacgc
    21901 cgttaccaaa agtgataacg acgtgaactg tctcttctat ttttatctgg cgctcttatc
    21961 tgaacacatt tcatcaatga ttttaccctc ccactataat tacagaacaa aaaaagaacc
    22021 tgcttcgtgg aaatcaactg tccatttccc aattggaata ccagcttttt tggcaacttc
    22081 accatcacca aatctcatgc cataccaatt actaccttca tccttaattt ctttccaacc
    22141 atatgacata gcttcatcta atgaatcaaa aatcatgaca ctattgccaa ttgttacagg
    22201 atctggctta agatcttctg gaagagaaat atcaatcata ccataatgat ccgattcctc
    22261 agtattattg tcattactag ctactggttt ggttgttcta ctggcttatt ttgatacaac
    22321 aagtactctt attcttcttt tacttctccg ttttcaattt cccattcaaa ccactccgca
    22381 aagtcttgct taaaaccatc tactcgatac ctattttgta aataggcatc ttctgacgtc
    22441 gtaggatcta aataggtagg tttattctct ggacgagcat agacacctac ccatgtttcg
    22501 taatctcctt caaaataact atctaccaaa tagccttttt ttctaagaat ctcccgaact
    22561 tgatttttaa ttattgatac ttgttcaaaa ccatcactca ttctatcacc tcaaataact
    22621 ctagattctc ataaatattc ccgacaattt caatcctatc aatgatctct aacaaatcgc
    22681 tacttatgct tccccattcg tataagaatt tcccttccgc aaaataaatt ttccataaac
    22741 ttcttcatga tcgtcccaac caatatcact ctcgaaaaat tccacgttgt tcttatcttt
    22801 tagctcagta aatgccataa gttcaacatg ttttccaacc tcgaaacaat cacaccaccc
    22861 atcaaccgtt acacgtgtgc cttcaatacc taagaaactt tgtcctcgtg tattaaacac
    22921 taaaactttc cgcatttttt gctcattaat taatcacgcg cgaaatctag gaatcacaac
    22981 ttattcgctt cttctttata aataaaaaaa ctccaataat cggaaattac tatcttttcc
    23041 tcattccttt tttaaatctt catacttctt cttatctttg tcctaaccta ataacacaaa
    23101 ttttgaagaa aaactcagta aaacctgatt ctttttttat cttcaca
//
LOCUS       DAYYCQ010000043        22841 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000043 DAYYCQ010000000
VERSION     DAYYCQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.043"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1137)
                     /locus_tag="ACHAYF_001651"
     CDS             complement(94..1137)
                     /locus_tag="ACHAYF_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8579092.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1514..2479)
                     /locus_tag="ACHAYF_001652"
     CDS             complement(1514..2479)
                     /locus_tag="ACHAYF_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8579093.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2713..3558
                     /locus_tag="ACHAYF_001653"
     CDS             2713..3558
                     /locus_tag="ACHAYF_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8579094.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3749..4936
                     /locus_tag="ACHAYF_001654"
     CDS             3749..4936
                     /locus_tag="ACHAYF_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8579095.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4942..5298
                     /locus_tag="ACHAYF_001655"
     CDS             4942..5298
                     /locus_tag="ACHAYF_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8579096.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5300..5638
                     /locus_tag="ACHAYF_001656"
     CDS             5300..5638
                     /locus_tag="ACHAYF_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8579097.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5714..5970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6033..7067
                     /locus_tag="ACHAYF_001657"
     CDS             6033..7067
                     /locus_tag="ACHAYF_001657"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8579098.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7060..7746
                     /locus_tag="ACHAYF_001658"
     CDS             7060..7746
                     /locus_tag="ACHAYF_001658"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8579099.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7770..8618
                     /locus_tag="ACHAYF_001659"
     CDS             7770..8618
                     /locus_tag="ACHAYF_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8579100.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8815..9588
                     /gene="sufC"
                     /locus_tag="ACHAYF_001660"
     CDS             8815..9588
                     /gene="sufC"
                     /locus_tag="ACHAYF_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8579101.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9601..10887
                     /gene="sufD"
                     /locus_tag="ACHAYF_001661"
     CDS             9601..10887
                     /gene="sufD"
                     /locus_tag="ACHAYF_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8579102.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10884..12119
                     /locus_tag="ACHAYF_001662"
     CDS             10884..12119
                     /locus_tag="ACHAYF_001662"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8579103.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12106..12576
                     /gene="sufU"
                     /locus_tag="ACHAYF_001663"
     CDS             12106..12576
                     /gene="sufU"
                     /locus_tag="ACHAYF_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8579104.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12581..13972
                     /gene="sufB"
                     /locus_tag="ACHAYF_001664"
     CDS             12581..13972
                     /gene="sufB"
                     /locus_tag="ACHAYF_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8579105.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14070..15212)
                     /locus_tag="ACHAYF_001665"
     CDS             complement(14070..15212)
                     /locus_tag="ACHAYF_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8579106.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(15263..16060)
                     /locus_tag="ACHAYF_001666"
     CDS             complement(15263..16060)
                     /locus_tag="ACHAYF_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8579107.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            16262..16561
                     /locus_tag="ACHAYF_001667"
     CDS             16262..16561
                     /locus_tag="ACHAYF_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579108.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            16826..17701
                     /locus_tag="ACHAYF_001668"
     CDS             16826..17701
                     /locus_tag="ACHAYF_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579109.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            17698..17916
                     /locus_tag="ACHAYF_001669"
     CDS             17698..17916
                     /locus_tag="ACHAYF_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579110.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            17903..20530
                     /locus_tag="ACHAYF_001670"
     CDS             17903..20530
                     /locus_tag="ACHAYF_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8579111.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            20545..20763
                     /locus_tag="ACHAYF_001671"
     CDS             20545..20763
                     /locus_tag="ACHAYF_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579112.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            22282..22596
                     /locus_tag="ACHAYF_001672"
     CDS             22282..22596
                     /locus_tag="ACHAYF_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579113.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(22649..>22841)
                     /locus_tag="ACHAYF_001673"
     CDS             complement(22649..>22841)
                     /locus_tag="ACHAYF_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579114.1"
                     /translation="VDYEYTADYLETPLQHDNNDINFRFIYNSKSKTWLVDQLSTINT
                     GFHELPKPDNETALVIKTF"
BASE COUNT         7892 a         3796 c         4657 g         6496 t
ORIGIN      
        1 tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca
       61 tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag
      121 taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc
      181 ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc
      241 aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct
      301 gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt
      361 tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc
      421 aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa
      481 ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag
      541 tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag
      601 aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc
      661 tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca
      721 aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc
      781 aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt
      841 tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa
      901 ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa
      961 agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag
     1021 ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa
     1081 gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg
     1141 atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag
     1201 tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat
     1261 aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa aaatttgaga
     1321 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt acgacctctt
     1381 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta caaaaacatg
     1441 aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt ttcacaacct
     1501 attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg ctatgttctt
     1561 taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt gcaaacagac
     1621 attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg ttgaagtaat
     1681 gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag gctgcatagc
     1741 ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg aaatacaata
     1801 cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca aagtttttaa
     1861 gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc ggacgtgtcg
     1921 gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat aagaacgcat
     1981 agcagatcaa tgaaatccaa aagaaaattt ttccaataag tgggaagaag ttcccggcag
     2041 ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa attgcttcaa
     2101 ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg atcacttgga
     2161 agataacagc gcacagccaa atgaatttga caattggtgc aatactttca tatgaaacaa
     2221 aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag gcgatgattg
     2281 gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca aaaatgaata
     2341 tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg tgatagtctt
     2401 taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg atcggaataa
     2461 ttttcaaaaa gttcttcata tataatattc tcctttttat ttaatagatt ttagtaccaa
     2521 gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag cagacagctg
     2581 attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca tttctattat
     2641 agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat aaaaagttat
     2701 ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga aacaagaaat
     2761 ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa tcaaatcaaa
     2821 cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa agagatgctt
     2881 ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga agaatgggaa
     2941 gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg gatcgttgct
     3001 tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat caaaaggttt
     3061 cctaaagtgc attttgagat cgaaatcacg aattctcatg aatcattaga acaagtagcg
     3121 aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg cgtacaaaga
     3181 tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa attgtggctt
     3241 gtaagagaag caaactctgg tgtttttcat tacacgcagc gttattttga agagatgaat
     3301 atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt gctgaaggaa
     3361 ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc aatgactccg
     3421 ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc tgatcagctg
     3481 ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc tcacagcgag
     3541 gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat acactaaaga
     3601 taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga aagttaattt
     3661 ttcccaaatg tctatggaaa gactttccat tatctaactg gaccgctata ataatcagca
     3721 gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt cgaacgataa
     3781 tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg gtctgctctc
     3841 attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag gcgtagggat
     3901 gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc atttgaattc
     3961 caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg taatgggact
     4021 cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa actggtttcg
     4081 attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca tcttgatgat
     4141 ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga aatcggactt
     4201 ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga tcatggccca
     4261 agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt tcctgatgtc
     4321 tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg ttggagcagg
     4381 aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttataaag ttggattcaa
     4441 atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata cgtcaggaat
     4501 gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt ttggtaaagg
     4561 attcaacgta agcaatatct atgtgcctgt tcgtgagtcc gatatgattt ttaccgttat
     4621 cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt ttattttaat
     4681 ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct atatcgcaag
     4741 cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa atatcggatt
     4801 acttccttta acagggatcc cattgccttt cattagtcag ggaggatcct cgattttagg
     4861 gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag cacctgcaag
     4921 atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg ttcaacatgc
     4981 aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt tgatatgatc
     5041 aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca agtaccaatc
     5101 cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa agatcaagta
     5161 ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat gctgatcaag
     5221 cgaccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga aaaaatttat
     5281 gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca acctttggat
     5341 cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag aagaacttgg
     5401 agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag atgaacctct
     5461 gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa cgggtacagt
     5521 ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt cagatgaaat
     5581 gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg aattataaaa
     5641 tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa tcataagcaa
     5701 tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg tttgaagaga
     5761 gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta gagcagagta
     5821 agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt atacggaaag
     5881 aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa tcaaggtggt
     5941 aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag gcttttttgt
     6001 ttgaaaaatt ataagagaaa gatgtgaatg gcatgtcatt aatccaacta acgaatgtca
     6061 aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt tcactaaacg
     6121 ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa agtacattag
     6181 tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta aacgatcaag
     6241 atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc ggtatgatct
     6301 tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag ctgccgctgg
     6361 aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg ttgcgtttag
     6421 tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga cagaaacaac
     6481 gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt gatgaggcta
     6541 ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta gcgatcaata
     6601 agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc cgcaaaatct
     6661 gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac gtattgactg
     6721 tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac atcgaaccag
     6781 aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc ggccgtttgg
     6841 caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa gcgattcgac
     6901 gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa gaaggttcct
     6961 ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa gcagaagtgt
     7021 tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag cttttcagaa
     7081 acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat ccttgataca
     7141 ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt tttgggattg
     7201 atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta tggaatcgta
     7261 tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt tttgatcatt
     7321 ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat tccagctctt
     7381 gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg tgaagtcagc
     7441 cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat catctggaag
     7501 gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac aacgatctcg
     7561 atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg aggtcttgct
     7621 tggcaggaag gctaccagcg tggtaatcta acagtgacat tcgttgcgac tttaattatt
     7681 ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa aacagacaaa
     7741 agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga tttgcagcag
     7801 cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc aaagattcag
     7861 caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg cacgcagaga
     7921 ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa atcgtcaaat
     7981 tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat gccaattact
     8041 tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac tttgtcaatg
     8101 ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa gatgtttccg
     8161 aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg ggacgcatca
     8221 tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat ctggaaacag
     8281 caacattcga tgatattgaa gaaaacccta agaacttgaa gttcaaccat acgatcgacc
     8341 catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca atcaacgcaa
     8401 acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt gaaaaggaca
     8461 actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt gacaaaatca
     8521 aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca gacaaatggg
     8581 gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa aaaagatgag
     8641 aaataagtct gtaatggctt atttctcatc ttttttatac ctattctaat ttagaaatca
     8701 aaattgttta ataataaata aaagatattt gattttagta aggaaggtca ttgcgcttca
     8761 ttctcaatta gtctaaaatg agtgagaacg aaagagaatt tgggggtata atgcatgtct
     8821 gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa aagaaatcct taaaggcgta
     8881 aacctaacaa tgaaaaccgg agaaatccat gccatcatgg gacctaacgg cacaggaaaa
     8941 tctaccttat ctgccgctat catgggcaat ccaaactatg aagtaacaga aggcgaaatt
     9001 ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg aacgtgcgcg acttggctta
     9061 tttttagcta tgcaatatcc aagtgagata ccaggtatca cgaatgcgga attcatgcgt
     9121 gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt ctgttatggc atttttgaaa
     9181 aagttagatg aaaaaatggc tttactaaat atgccagaag aaatggctga acgttatctg
     9241 aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga tcctgcaatt gttgatgtta
     9301 gaacctacat ttgcaatttt agacgaaatc gattcaggtc tggatatcga tgctttgaaa
     9361 gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt tcggtgcatt gatcatcacc
     9421 cactatcaac gattattgaa ctatatcaca cctgatgtcg ttcacatcat gatggaaggc
     9481 cgagtagtca tgacagggaa tgctgaccta gctaaacgtc tggaagcaga aggctacgct
     9541 ggtatcagta aagaattagg aatcgactat aaagaagaag aagcgtaagg gggagcaaga
     9601 atgaaagaaa aaaactggct cgattatctt gacgcggtaa atgatttttc tttaagtaaa
     9661 ggcgaacctg attggatgag aaccttccgt caagacgcac tagcgaaagc agatgaactg
     9721 ccattgccgc atatcgatcg ggtgaaattc catcgctggt cgttatttga tgtgaaagaa
     9781 acacaaacca tcagtgaaac aggcacgatt ccggcatttg atgcaatgaa agacaatcct
     9841 gtgcttgtcc aacaaggttc atggacgatt tttgaacaac tgcctgtgga attagcagaa
     9901 aaaggagtca tttttaccga cttgttcact gcaatgatcg aatatccaga actagtccaa
     9961 gaatattata tgaaaaaagc agtaaacatg aacgaagacc aattgactgc actccatgta
    10021 gcttttatga acagcggtat tttcctgtat gtgcctaaaa atgtcgtgat cgatgaacca
    10081 ctcgagtcct tattcattca agatggcgca tccgatgagc acttcttcaa gcatgtgttg
    10141 atcgtggcag atgaacatag tgaattttct tatctggaac gtttccagac aaccaaagaa
    10201 caagtagcaa aatcatcagg taatatcatc gtggaagtca tcgctaaagc tggttcaaaa
    10261 atcaaatatt cagccgtaga tcaattagga gaaaatatta cttcttatat gaatcgccgt
    10321 gggcacattt tacgagatgc ttctgtcgac tgggcaattg gtgtcatgaa tgacggtcat
    10381 gtgatcgctg actttgactc agatttagca ggagaaggtg cacacgcaga agtcaaaatc
    10441 gtggcaatca gctcgggcag acaaatccaa ggaatcgata cacgagtaac caataaagcc
    10501 cctcatacga tcgggcatat tttgcaacac ggagtgatcc gagaaaaagg aacattgacc
    10561 tttaatggta tcggccacat cttgaaaggg gcaaaaggtg cagatgccca acaagaaagt
    10621 cgtgtcttga tgctttctga taaagcacgt ggtgatgcga atcctattct tttgatcgat
    10681 gaaaacgaag taactgctgg acatgctgcc agtgtaggac gagttgatcc agaagaaatg
    10741 tactatctga tgagtagagg attgcataaa gaagaagcag agcgcttagt gattcgagga
    10801 ttcctcggtt ctgtcttaac agcaatccct gtagaacaag ttcggaaaga attagtcgaa
    10861 gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa gctacgcaat gattttccta
    10921 tcttatttca agacgtcaat gatgaacctt tagtttatct ggataatgca gcgacgaccc
    10981 aaaagccaaa acaagtactc caggcattag cgtactatta cgaacacgat aacgccaatg
    11041 ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca tgattatgaa caagcaagag
    11101 aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga agtgctgttt actcgtggta
    11161 cgacgacaag cttgaattgg gtagccagaa ccttcggcga ccgctatatc aaagcaggag
    11221 acgagatagt gctctcttac atggaacatc attctaatgt tatcccgtgg cagcagttgg
    11281 caaaacgaac aggtgctgtc ttgaaatata tcgaactaac agaagaaggc ttcctggata
    11341 tggaggatgc agcgaaaaag attacggaca aaaccaaaat cgtctccatt gctcatgtat
    11401 ccaatgtctt aggtgtcttc aatcctgttc aagaattagc tgaacttgtc catgaaaacg
    11461 gcggggtcat cgtagtagat ggtgctcaat ctgccccgca catggccatt gatgtccaag
    11521 cactagactg tgatttcttt gctttcagcg gacacaaaat gtgcggacca acaggtattg
    11581 gtgttttata tggtaaacga aaatggttag aagaaatgga acctgttgaa tttggtggcg
    11641 aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa agaactacct tggaaattcg
    11701 aagcagggac accaaatatc gccggcgcta tcgcattagg ccatgcaatc gattatttgc
    11761 aaaaactcgg tatggacaat atccatcgat atgaagaaga actagccgct tatgtgttgc
    11821 caaaattaca agcaattgat ggattgactg tttatggtcc acaagacccg aattttcgga
    11881 caggagtcgt tgcattcaat ttggatgggt tacatcccca tgatgtcgcg acagctttag
    11941 acatggaagg ggtagccgtc agagcaggtc atcattgtgc gcaaccgcta ttgaagtacc
    12001 ttgaagtatc agcgactgct cgcgcgagct tttatttcta caacacaacg caagacgccg
    12061 accgtttagt tgaagcaatc atggcaacaa aggagttttt ccaacatggg actatctaaa
    12121 ttagacaatt tataccgtca agtgatcttg gatcattctt cccatccgca tcatcgtgga
    12181 agcttgactg actcgacaag caagatcgaa ttgaacaacc ccacatgtgg agatgtgatc
    12241 gaattgcaag tcgatgtaaa agacggaatc atcaaaaata tcgcctttga cggcagtggc
    12301 tgctccatca gtacggcaag tgcctcgatg atgacagacg ccgtcatcgg aaaatcaata
    12361 gaagaagcag aacatttaac tgtagacttt ctacagcttg tccaaggaaa agaagtcaaa
    12421 gatcccgacc aattaggcga tgctgctatg cttggtggtg tagctaaatt tcccgctcgg
    12481 atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag caatggaaaa cgatggtacc
    12541 gcagctttcc atcattacca caaggaagag gagtgagaac atgggcgtac cagaattaga
    12601 agaatataaa ttcgggtttc acgatgatgt cgaaccggtt ttcagtacag gagaaggctt
    12661 gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc gaaccagaat ggatgcttga
    12721 attccgtctg aaatctctag aaacatttaa caaaatgcca atgcaaacat ggggccccga
    12781 cctttcagac atcgatttcg atgccatcaa atattaccaa aaaccaagtg acaaacccgc
    12841 tcgtgactgg gaagacgtac cagaaaaaat caaagataca tttgaaaaga ttggtatacc
    12901 agaagctgaa cgagcttatc tagccggtgc ttccgcacaa tatgaatcag aagtcgtgta
    12961 ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc tttactgaca ctgactctgc
    13021 tttgaaagaa taccctgaat tattcaaaga gtacttttca aaacttgtac cgccaaccga
    13081 caacaaatta gcagcgctga attcagccgt atggtcaggt gggaccttta tttacgtgcc
    13141 aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc cggatcaatg cggaaaacac
    13201 ggggcaattc gaacgtacgc tgatcattgt tgacgaagga gcaagcatcc actatgtcga
    13261 aggttgtacg gcacctactt attcaagtaa cagcctgcat gccgccatcg tagaaatctt
    13321 tacaagaaaa gatgcgtatt gccgttacac aacgatccaa aactggtcag acaatgtgta
    13381 caacttagta accaaacgag caaaagcata cgaaggcgca accgttgaat ggatcgacgg
    13441 aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt tacttagatg gaaaaggtgc
    13501 acgaggcacc atgctctcca tcgcctttgc aggagcaaac caaatccaag atacgggtgc
    13561 aaaaatgatc cacaatgcac caaatacatc tagctctatc gtctctaaat caatttcaaa
    13621 agacggcgga gaagtaaact atcgaggaca agtaaccttt gccaaaaaca gtgcgggttc
    13681 gatctctcat atcgaatgtg acacgatcat catggacgaa tggtcgaaat ccgatacgat
    13741 cccattcaat gagatccaca acagccaagt ctctttagaa cacgaagcca aagtctcaaa
    13801 aatctcagaa gaacaactct actatctgat gagccgaggc ctgaccgaat ctgaagcaac
    13861 tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa gaacttccaa tggaatatgc
    13921 agtcgaactc aatcgattga tcagttatga gatggaaggg tctgtaggtt aataactatt
    13981 tcattgctcg aaacgttgat attaaagcgt ttcgagcgtt tttttatttg tattttaatg
    14041 gttggctacg tttttggcta cgtttggaat tattcgccca taaattttgc aaatcggtca
    14101 ccagttttct taacagattg tggtgtgaca tgagcataga tattcatggt agttttaata
    14161 tctttatgac ctaatcgttc ctgtacttct tttatcgacg caccactttc aaacaataag
    14221 ctacagtggg tatgcctaaa tccatgtgga gtaatttgag gctccatcgg tgtctttttg
    14281 taaatccagt ctaaccaatc gtttactact tgggggtagt acaacttgtt tgtgtttgta
    14341 gtgaaaagaa actgttttgg tgaattcgtg ttatagccat ataagatcat tattttcatt
    14401 tgttcttgtt gccaaatacg cagttgttta agtgtaaaat catcaagctg aatcgttctt
    14461 gatgaatttt tagttttagg tacttgtaga actacttgat taaactcgtc ttgagcgata
    14521 gttttaccaa ttgttattgt tttattgaaa tagtctatat cttcccattg aagcgccaat
    14581 gcttcgcttt tacgcattcc tgtataagct aataaacgga aaaaagtgta cagtttaact
    14641 gaattatttt ctttagtatt ctgtaaaaac agttgtaatt gatctttcgt gtaaaacttc
    14701 aagggttgtt cttcttcaat ttttcttgga agcaatgttt tagacatagg attatcctta
    14761 cagacttcca ttgctactcc aaatttaaat atttgagcag ttgtttttct caagaaacca
    14821 tattgtttta atgttttgtg ccattcattc acctgttttt ggcaatagct aacacttatt
    14881 ttatccaatc gtaattcccc aaaagcaggt aatatatggt ttttgcaaaa acgttgagca
    14941 acggcaacag aagacggttt tactcttgtt ctgtagttat caatccataa ttcatacaac
    15001 tcttgaaaag ttgtaacatt gattttaaac tcttgttttt ctttaaagtc tacaagtagt
    15061 ctattaagtg ctgtttgcgc ctcagctttt gatttgaatc ttctacgctt tgtacgtata
    15121 tcttttccag tcttgggatc tttccctaaa taagtttcaa atccccaata tttttcgcca
    15181 ttttgtaatt tataactaaa aaatgttgcc atttgattct ctccttactt cattattgca
    15241 ggtagagaag ttatttgaag aatcaattat ttttaaattt atcgtgatta tcagttaaaa
    15301 attttgcgat tatactattt ttctcatatg ggaaattgta ttttttacta agatctgaaa
    15361 taaatttttc tgtttcaaat tggtttttag taattttata aaagaaatcc tcgacagatg
    15421 aatactttga aatatcttta taggctaagt ccatttcgtc taatagctgt tttaaagaat
    15481 caatcattct ttctttttca ccgtatttga aatcaccatt tcttatcttg tcttgaagtt
    15541 tactgttggc tcttttgaaa aatagctctt tagagataga gtgtctttgg ttagcgtact
    15601 cttcaatcag aggattgata ttattagcaa gtatctcatt tagataaatt tcaaaatctt
    15661 ttgagtaata ttttgtaaat atatttttta ttataggatt caataactca tagtctttat
    15721 ctagcgaaaa cgtatcacta tatctttctt gaatgtcttt aaagactttg tgaggtattt
    15781 ctgggaagtc cttaatatat agctcatacc ctatatcgat aaggtatgaa gttatatatt
    15841 cttctaaagt tccccattta atccagtcaa ttgttgaatt agtgatagtt gataataatt
    15901 tcagcttttg tggatttggt aaattggtcc cacgttccca gttggatatg gtcgcacgtg
    15961 gtgaattatc tattttcctt ccaagttctt ccatagtaaa accacatgaa aggcgtaatt
    16021 gtttgattcg ttttcctgct tctgaaggat ttggcttcat cttttttctc ctttttattt
    16081 ttaaagatag tatatcatat ttataaaata aaagtattta aaagtattta ctttttatct
    16141 ggagtatgat actttattct tataaataca aataaataca tttttttacg caatttgtat
    16201 ttatttgtac ttataaaggt agaggagtta tagaataatt aattaaaaag gagaggtttt
    16261 tgtggaagca ataaaattaa tgatttcaga agaacaggag gaaatgtttc taaagcagtt
    16321 ttatcaaatt gctacaaaag ctattgaaaa ggctcaagaa aatgcgggga tgacaaggga
    16381 atatctcaat caaaaagaaa tggcggaata cttaaatgtg agtgaaaact ttttaaaaaa
    16441 tgctgtgaga aatgagggat tgcccgtagt tgaattaggg gcaagaaaat tttattcgaa
    16501 aaaggcagtt aatgagttta tgtttgctcg aatgaaaaga ggggaaaata ttggaagatg
    16561 atgaagtaga aattataaaa aaatgcttta aaaaaagaaa aaaagtgttc ccaaaagttt
    16621 gcagactggg ggaacactca ataataaagt gctgagaaaa ctttatttat agctattata
    16681 tcaaaaaaga aggtgaaaag gtgttttctt ctggaaatgt tgtagttgac aaacttagag
    16741 aaataaattt tgacggaaat gtaatccaca cacatggtac gatcaattaa gaaaaaaaac
    16801 gaaaaaaggt gttgagaagc cctatttgaa tgcaatagtt atcttagcag aaattaccta
    16861 ttggtataga cctaaaaaaa tttttaacga tgatggtcag ctacttggtt atcaaaaaaa
    16921 gtttcttgaa gatattttgc aaaaatcata taagcaatta agcaaaaaaa caggattatc
    16981 agaacgagct ataaaagatg caattgtgta cttagagaaa aaggggatta tcaaaagggt
    17041 ttttagaaat atttattatg agaaaagagc agttacatta tcaaatgtta tgtacattgt
    17101 tttaaatgta cataaattga tagaaattac ttttcctact ttggattgcg aaaaaaagga
    17161 gtatatgccg aaaattcgtc agaggggtat cgaaaaaaag agatatgtct ctttaaaaaa
    17221 aggtagaggt atgtacaaat ttgtatatac gtatacaaag actacttcac agaacatatc
    17281 aaagactact ctcgttatgg aagaagagga tattaatata aagaggattt acagtaagat
    17341 tagtgttctt tatgacgcaa ataacgaaga tggtcgaaaa ttatttcaag tgtatcacta
    17401 tttgagaaac gctaatgtag atacagaaga tatcagaaaa atcattgaat atttatctga
    17461 aaatactcag tatcttattg ctgagtgtat tataaaacaa catgaagcat gcttttttca
    17521 aagaaaaact gaagaagggt tatacgatta tgtaacatat tttttgaatg gacttcagaa
    17581 aagatataaa agtcaatttt tagaaaatgt agagcctata gatcagttca aaaaagcttt
    17641 cgaaatcaaa gagttacctg aaattactat gcataattgg ttgaaaggag aagaatcatg
    17701 attaaaaatc gcttaataga tcaaatgtgg tgtcttgatc aaaatacaga tactctcgaa
    17761 ttaatcgaga gtatctgctt caacacagta gttctcgatt taagagctga aaatgacaat
    17821 tgtgtaactc atatcataat taatcaaaaa atggctcagc agctctctga gagcctaaga
    17881 aaatgggctg aaggagaaaa ctatgaaagc aattaaagaa aaaatcgtta gtagaaaaat
    17941 aaactggtta gcactcagtg tacttgtttt aatgattagc gttgttttta caagtaatat
    18001 tgttgaagct agcattttca attggtttga tgacacaaaa agtcaaacag agtttttgaa
    18061 taattctctt tatagacctt acctaaaaaa atacaatgga gatttcctta gtcagttcgg
    18121 gatttggctt ggatgggctg tggtaaaagg catgtttact gtgactgatt cgattcaaaa
    18181 tatgattcca gatgtgttgg atttgttcaa tttcattgaa agtacagggc taaataatgt
    18241 ttatcaatca gtcataaata caattgtcgt tggattaatg atcctttcat tgatgtttgt
    18301 tggctacaag atgattactg gaaaaggaac aatagattta agatctgttg gaatgaatat
    18361 agtgatgtct gtagcgttaa ttcttttaat gccaacaatg atttctagcg gtattcaatt
    18421 tgccaaaata ttttacaatg atgctactac gattacaaat tcagatgacg gagtcgcttg
    18481 gtcattgata aaacaaggag taacagatct ggcatatatt aataaaacag atcaatactc
    18541 aagcattgat aaaacggagg atcgaaataa gttaacacga aaaaattttc aacagacaga
    18601 tttaacacaa gtgttgacgg ataaagtgat tgataaattg gaaaaagaaa atccagctgc
    18661 ggataatctt cgctatgaat tagttgaaaa ttctaataat gaatttgttg ccacaaagtt
    18721 ttctgataac ttcttatcaa cattttctga tagcttgaaa tcaggttatt atcgatatca
    18781 agcaaatctt tgggggattt cgattgggtt gacagcgttg gcaatcgcct atgtgttcag
    18841 cgcgtttgtg atcattacag caatattaga acttgcattt aaacgtgtgt taggtgttct
    18901 tgtttttgcg acagatattg aaacaggaca acgttcaaaa gttgttttat ccgatatttt
    18961 acaatgttac ttaacagttg gatttcaagg gtttggacta tctatgtttg ctatgtttat
    19021 caatttttta aatgctggac aaggtatctc cacaaatatt tttataaaaa caattgctta
    19081 tatctgtgct gtgtttgtgc tgataaaagg cagtggaaca gtcatgagat actttggtgt
    19141 tgatattgga ctcaaagaag gctatgggca acttgcttct gcttttggta tgggcgcaat
    19201 gctgtttaga aaaggatcaa ctggatttaa tcgtgctaaa ggaagcggaa atggaaatgg
    19261 atcagacagt ggagaaggag aagatcgaaa accagaaaag aatttcggcg aaactctatc
    19321 aaaaaaagct ggcaaaactg gaagagcttt gggatatgcc catgaacgtg gattatcagg
    19381 tttagcttca gatggagcta caatggctag tgaaagagca ataaaaccat ttaagagtat
    19441 gcgtgatatg gctaatgata ccaagaataa gttcaaagaa ggtttagatg atggtacagt
    19501 gtccgctatt aataaaaata gcaaacctat gttagctaaa aataaagaag atgaaactgg
    19561 aaaatatgct gattccatgc caacacgtct ttcggataga aaagatggtg ctaaggttga
    19621 aaatgccgat agaattatgt ctagttctga acgtatgcgt gaagcaatga aaaataatgc
    19681 tgaatcgaat aataatcctg taagtgcaat tcagcaaaaa gttcaacaag atattgaaga
    19741 acgtaaaaat gctatgcacg gtcaagctaa gtctgctgaa gaattaatta atcagaagcg
    19801 acaagaagct aaatacacgc ctgaagcaat gaatcgtgag gaaatgttaa ggaaacgtgt
    19861 agaagggcgt acaggagcta atacggacga aaaagaagcg ttgaccaaag aaagaatcca
    19921 agaagctaaa aactcaaata ccggattaga aaaattggtc aaagaaaatc tacaaaattc
    19981 tacaagtagt caaggtggcc gatcagtaga tgtccgagaa aatattcaag gttcattgaa
    20041 tggtcatgtc ggacggaatg ttgatgtaag agagaattta caaagctctt caaatggtca
    20101 aggtactaag acaattgatg ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt
    20161 taaatcaatg gatgtaagag agaatgtaca aaattctaca agtagtcaag ggtttaaatc
    20221 aatggatgta agagagaatg tacaaagttc tacaagtagt caaggattca aaacagtaga
    20281 tgttcgggag aacgttcaaa aagatagtgg agaaatgaaa gaaaaaacac aaaaaatcaa
    20341 tatcgttgaa gacagaaagt cagcgaaaaa atttgattca aatcatgaaa cagtgattat
    20401 tgatagtgaa atccgtgaaa atgataaggg atcaaaacca agacgaagat ttacctatga
    20461 agacaacgag ttattcagag atacacttaa tgatcctaat ccgcttttcg ataatctatt
    20521 aaaaaaataa aagggggaat tgtcatgaaa ttaattctaa agattagtac gcatgatttc
    20581 ttagaacttg acgaattatg tgaatcagta aagaacgcaa caaatgaaaa aatcacggtc
    20641 actaattata ttgaactgat gattcaagaa gaattaaaga tgaaacatga gataaaaaac
    20701 aacttgaaat attgttatct agaaaaaagt gaagagataa tccatgaatt aaatggcttt
    20761 taaaaattgt atttgacatg aatttacgac aaggaattag tggggattaa gcgattattc
    20821 tcttccactt tagagaaata atgggttaaa aagccagaat acgtagagca atattgacaa
    20881 cctataaagg agctattgtt gaaaattgaa gtaacaaaaa gataaagaga ggtttacatc
    20941 tatcagatta aaagtgatag tacacccatg aaacctgcaa aagagttgaa cctaatgtat
    21001 atcggtttaa ctctttttat ttgcaaatta aaaaaatatc accgaagtga cgaatgaaac
    21061 tatgaccaaa ttaagcaaga aaatatatct tgcaaataaa gtagtataaa aagatattga
    21121 taaagccaat caaaactttg aatttcaaac gttagataat caaacagata caaaaaatga
    21181 aatcagttct acgcgtacga ttattaaaaa taataaattt tcactagaag aaaatggaaa
    21241 taaaatcaaa taattaggaa gaaaaaacaa agcaattaga aaagcctgca agcaaatttg
    21301 agtaatcgag atccaatttt tttaaggaag tttaacaaaa atatgtaatt ctattatcac
    21361 tattaaagag aaagttggag cggaatataa agaaaagaga aaagtaatta aacgcaaaaa
    21421 aatgtagaac ttaaaaaata taaagaggaa caaccgttaa aattaggaat gatgttgtgt
    21481 aaacattata attgctatga tataaatgaa gtgttaaaga aagcaaattt aaaccaattt
    21541 tgtgaacaga aaagccttag tcactatgac caaaactacg gacgaaacta tgaccaaaag
    21601 taggtactaa attatgaccg aaaataggtc caaaatggat ttttttatga tatcaaaaat
    21661 tatgaaatga ttttgaaaga aaaccagttg gcacagccaa cttagccacc ttcctgtagg
    21721 aaggtgctat cagttttccc ttatgctctt ttctttcttt tttgtttcat atgtcaaatg
    21781 ccattttgaa atgtaccatc ttaagaaatt tatccttaaa aacaattgcg ttattggata
    21841 aaaagatgct acattatctt aaaaaaggaa tcaaatatga tgaatttagt ggctagcata
    21901 tcgtgtgatt aagatacttt atagtgtaag ttgaaggaga aaaaatattt aaaatatact
    21961 tcccatttca gtgggaagta aaattaatct tgttgtttct actagtaatg tatctttttg
    22021 gaaactatgt aacgatgtaa acaagatttt tttcagaaga aaaaacgata aaagttgtga
    22081 taaacgattt aaaaaatagg agaaaatata tcaagcgcat ggtatgaaac gcacatttaa
    22141 aaagattcga ctaactaaaa aaataagttt acaaagcatt gttgtgtatt acaagtgatc
    22201 tatacactga aaaatgatag agaaaatctg atacgtccgc aacaatggac aattgaaaat
    22261 gaaagtgagg aaattggaag aatggctgat gaaaataaag ttgttccgct tgaaacacta
    22321 gtgataaata aggaagagtt tgagcaactt tttcttgatt tttttgttca atatgacagg
    22381 atttatgatt ttagttcgtc actagattta tttgaattag aggcagaaat aatagaaaac
    22441 agtcgagagg agaatggtaa aatcatcatt tcagtagaaa aattcaatga attattctca
    22501 gaaacatttg aaatagatac agattattat caaccacaag aagttttcca atcatttaga
    22561 aatcagatta ttcgctcagg taagaccgta aactgaaaaa caataaaaga aacgttgaaa
    22621 gaataattat tccttcaacg tttctttttt agaatgtttt aatgaccaaa gcagtttcat
    22681 tgtcaggctt aggtaattca tgaaatcccg tatttatagt gctcaattga tcaactaacc
    22741 atgttttact ttttgaattg tagataaatc taaaattaat atcgttatta tcgtgttgaa
    22801 ggggcgtctc caaataatct gctgtatatt catagtcaac g
//
LOCUS       DAYYCQ010000044        22662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000044 DAYYCQ010000000
VERSION     DAYYCQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.044"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..3321
                     /locus_tag="ACHAYF_001674"
     CDS             43..3321
                     /locus_tag="ACHAYF_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8579115.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3862..5769)
                     /locus_tag="ACHAYF_001675"
     CDS             complement(3862..5769)
                     /locus_tag="ACHAYF_001675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8579116.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5899..6774
                     /locus_tag="ACHAYF_001676"
     CDS             5899..6774
                     /locus_tag="ACHAYF_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8579117.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6854..7828
                     /locus_tag="ACHAYF_001677"
     CDS             6854..7828
                     /locus_tag="ACHAYF_001677"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8579118.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8008..9153
                     /locus_tag="ACHAYF_001678"
     CDS             8008..9153
                     /locus_tag="ACHAYF_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8579119.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9290..9634
                     /locus_tag="ACHAYF_001679"
     CDS             9290..9634
                     /locus_tag="ACHAYF_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8579120.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9905..11191
                     /gene="efmA"
                     /locus_tag="ACHAYF_001680"
     CDS             9905..11191
                     /gene="efmA"
                     /locus_tag="ACHAYF_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8579121.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11329..11928)
                     /locus_tag="ACHAYF_001681"
     CDS             complement(11329..11928)
                     /locus_tag="ACHAYF_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8579122.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12332..13453
                     /gene="mnmA"
                     /locus_tag="ACHAYF_001682"
     CDS             12332..13453
                     /gene="mnmA"
                     /locus_tag="ACHAYF_001682"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8579123.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13699..15120
                     /gene="arcD"
                     /locus_tag="ACHAYF_001683"
     CDS             13699..15120
                     /gene="arcD"
                     /locus_tag="ACHAYF_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8579124.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15134..16252
                     /locus_tag="ACHAYF_001684"
     CDS             15134..16252
                     /locus_tag="ACHAYF_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8579125.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16312..17604)
                     /locus_tag="ACHAYF_001685"
     CDS             complement(16312..17604)
                     /locus_tag="ACHAYF_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8579126.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17754..18314
                     /locus_tag="ACHAYF_001686"
     CDS             17754..18314
                     /locus_tag="ACHAYF_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8579127.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18443..18820
                     /locus_tag="ACHAYF_001687"
     CDS             18443..18820
                     /locus_tag="ACHAYF_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8579128.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18753..20825
                     /locus_tag="ACHAYF_001688"
     CDS             18753..20825
                     /locus_tag="ACHAYF_001688"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8579129.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21036..21983)
                     /locus_tag="ACHAYF_001689"
     CDS             complement(21036..21983)
                     /locus_tag="ACHAYF_001689"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8579130.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22229..>22662
                     /locus_tag="ACHAYF_001690"
     CDS             22229..>22662
                     /locus_tag="ACHAYF_001690"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8579131.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7334 a         4142 c         4810 g         6376 t
ORIGIN      
        1 ctttaagtaa acgctttcta atttgttacg ggaggaatac acttgttaaa aaaacgtata
       61 aaatacagtg cgctaatgat gaatctcctt atgctcagta ccccggtttt aatttctgtc
      121 gttgaagttg ctgctaacga acaacagtct gtgaacgaag aaaatgcaca cgtgaacatt
      181 gaagggatgt caacaatgga ggaaatacca agaactagtg aagatatgga tacggaagaa
      241 ggaaatgatc ctatgatgaa agaatcagaa aatgaaaaga caaaaaagca atctgaagaa
      301 gcaaatgaag agtggaaagg ggttgttttc ggagaaagca caagtgctag taggtatgcc
      361 attgaaccat tagaagacgg cgtgcggctc tcttccacga ataacggcgg aaaattccag
      421 tcaagcggat cagatggaat gacctattat tatcaagcaa tccccaaaga catcaacttt
      481 aggatgcggg cgacgatcga aatagaatct tggacctata cgaatgccca agaaggattt
      541 gctttaatgg taagagacgc agtaccaaaa aatcatttgt ttgggcaggc attttattca
      601 aattcatttg ccatactagg cagtcggatc gaatatgtct gggactcgga tagacaagaa
      661 gtggtcaaca cgagcggcag caaatatacg atgcgacttg gattggggac acgggcaatc
      721 acaggaatat catctgaaga tccgcaagct gcgccagctc ctgggagtgt ctcagtggaa
      781 acaacacctt tagagacttc agcagggttc ttggaaaaag aaacaggttc ttataatata
      841 ttcggaaacg gtcatgggaa tctgtttccg gccacgaact cgattactaa actggacgta
      901 gagatgaaaa aaacgaatac tggtttagag gcatattatt acgatcctga aacaggagaa
      961 gaaatggctt ctgacatcat gtatgactgg gaaaaattat acgcttcaga tgaatcggtt
     1021 atttatgcag gatttgctgc agcaaggaat atgacgatca aggtagaagg aatcgaagtt
     1081 gcttactcag atcctgcgac agatcctcct gggcaagaaa gacctactcg cttgattgat
     1141 ccgatttaca ctgtgacctc tagtaatcat tcaggaaacc aagatcatac tatgtccttt
     1201 agagcaaatg ctgacggatt attgacaatt aaaaataaag caacaggaga ggtgcttaag
     1261 aacgcaaaaa acttggctat cactgcgaat cgggaatttg attatcagac aaaactgatg
     1321 gaaggaacga acgaatttac tttcagtttt acgccagata aaaactatcc gccagaagaa
     1381 tacgtagaga tgactagtta tgaaacaaaa gagattcttc atacagtgga ctaccgtaaa
     1441 cctaaatatt cgacagttta tgtaacacca gaagggtcag atgcaggaaa cggaagcaga
     1501 caaaatccat tatctctgac acaagcattg agatacgcat atgctgggca gacaatcgtc
     1561 atggcgcctg gaacttattc cttcgaaaga ggattgacga ttccaaaagg cgttaacggg
     1621 acaagagaaa acccgattca gttgattgca gagaaaaatc cagaaaacaa gcgtcctgta
     1681 cttgatttta aaggaactgg aaatgggatg acactgttca gtcactactg gggtttacga
     1741 gggtttgaca ttacaaatag tgcagctatg caaaaaggat tacagatttc tggaaatcat
     1801 aatttgatcg aagagattca tgcttatcgg aatggaaata caggaatcca aatcagcggt
     1861 agctcaaatg atcctttcga agcatggccc gcgcataact tagtcaagaa ctgtacatct
     1921 tttgaaaatg ccgatccagg atttgaagat gccgatggat ttgcggccaa actgacaata
     1981 ggagaaggaa atgtatttga cggttgtatc gcgtatcata atgcagatga cggttgggac
     2041 ctttttgcta aaaatgaaac aggttcgatc ggaaaagtaa tcatcaaaaa ctcagttgct
     2101 tatcgaaacg gctgggtacc aggaattgag ggagaaggta acggtaatgg attcaaaatg
     2161 ggcggttctt ctttaacggg ccctcatgaa ttgatcaata gtttgtcttt cgaaaatctt
     2221 gcgaaaggaa tcgattccaa tagcggaaca gatattatcg tcaatagcag tacaagtttt
     2281 aataacggtt catataatgt ggctctttat acctcttcag caggaaacac agactaccat
     2341 gcttctggta tcttatcttt ccgaacagag aatcttgaga tgagagagca gatccgccca
     2401 ttgaatcaaa acgaagatca aatctaccac tcgttgaact actattggaa tgaacgagag
     2461 aaacaatcag aaaatactct tggacatacc atatcaaaag attggatcga gtctctaaat
     2521 acatgttcga aagaaggtca gcagcctgtt acgcgctatg aaaatggaag tatccatgtt
     2581 catggactga tgcagattaa accggggaat cgtcaaacag aaaaggaaag agtggatggt
     2641 cttccattat ctgtaggtgc tatttttgac ggagaaacct caccgtcttc ggaatatcca
     2701 gagtatgcaa tcgtacaaga accaaatcag acaaaaccaa atgaacaaaa taatccgatt
     2761 attatacaag aagcaaatgg tgtggcggag aatgcagaag gaaatatcat tgaactgaag
     2821 ccttttacag tttttgtgtc aactttcaca ccaaatcaag gaacgcctgt ctttgaagat
     2881 atgcgttatt tcccagaaaa gatccaaatc gttgaaacac agtcaagtgc tcaattttct
     2941 aatcataccg ctattttttc agcaccttca aaaggagaat atacgctaga agtcacttat
     3001 cgttcggaaa tatttgatgg ggaaaaatgg tctgctcata cagaatctgt tacccagcaa
     3061 aaagcgatca ctgtgcgaga aactgccgat gtgcccccgg gaaatgacaa accagaaaga
     3121 gaaaatccag gaaatgaatc accaaatgga aaaccaaatg gaaaaccttc tcatccggat
     3181 aggacagcaa aagaaagtga aaaaaatggc actcaaggac tcccaaaaac gggagaattt
     3241 aataacccgt tgctagcatt agctggagga attttgttaa tcggtgtagt tgtttatgtc
     3301 atgaaacagc gaaaaaaata agtggaattt ctccttttat gatgtaaaac aattgactga
     3361 ggaaagtaaa aatagaagga aatgagacca atagatgtct cacttccttc tatcttttta
     3421 tagatgaggt tctccttgct gcttttgatt ggctgttctt attacttgat gcaggacagc
     3481 tttttcacaa tctctccact aaacggtgtt caatcagctg accctcggta ttagctcaac
     3541 aaacggcaaa atatgaggaa ctatttttcc gtttttttct ctaatactca ggtcaggacg
     3601 ctgatttcac aacctctcga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
     3661 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttactact tgaaactaag
     3721 cagctttttc acaacctcta atacatggta ttccagaagt aacttcttcg aaaaataagc
     3781 cgaaatgtca caaaaatttg agaagcgatt ttcccaaatt ccttcttatt tcttgaagct
     3841 gaccacttct ggcacaacct cttaccagat tcgcactctt ttttctggcg ctagatacat
     3901 agcgtcttgt tccgtgatac caaatgtttc atagaattcg tcaaggtttt ttggttgcac
     3961 gtttgctcgg agtttagcag gggcatggac atcgatttgc agtaaaagtt gttggtattc
     4021 tttttttgct tttgtacgcc aaatcgttgc ccaattgaca aagaattcct cgatagaaag
     4081 atctgattcc gtttttgctg cttccaatgc acagcttaga ccgcctgcat cagcaatatt
     4141 ttccgaaacg gttaattttc cgtttacttt tccgtcagca aaaggcagac catcgaattc
     4201 ttcgatcatt gcttgtgctt tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt
     4261 gttcaagttg ccatattcgt caaataaagc gccgttatta tcaaatgcat gggaaatctc
     4321 atgagcgata acagctccga ttccaccata attagcagag cttgattgtt ctagactgta
     4381 aaatggtgct tgaaggatcg ctgccggaaa gacgatcaca ttccgaaatg gatgataata
     4441 agcattgacc gtgtcagcac tcatttccca ttctgaacga tccactggtt tgttccattt
     4501 gctgaaacga tcttttaatg tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc
     4561 accctgatct gcaggtactg ttttgaactg gtcgaataat gctgggatct tatcggggta
     4621 gcctacttgg atacctaagt tatttaattt tatgacggct ttttcttttg ttgcatcccc
     4681 aagccagtca ttttcttcta gacgtttttt ataaacggcg atcatttttt tcaccatttg
     4741 ttctacgtct tttttagcag cttcgccaaa atattttttt ccgtagtaat cgccaactac
     4801 ttgatcgaat tgtccagagg caaggtaata cgctgctttt ttctgaggca ttgcttcgtc
     4861 tgttccagac aatacgcgag aatagattcc gctgacttgg cggaattctt ctgatagata
     4921 accactaaga gaacgtactg ttttaatgat catccagctt ttgaataacg gaaaatgagc
     4981 ttctgtcagg atgtcattca aatgatcgaa gtacactgga tcagttacga tcactttatc
     5041 tggagttgtc ccaatcaaac ccgtgacaag ttttttcaag tccagtttat ctgtgtattt
     5101 aataaatgta tcaaaagatt gcggattata cattttgctg taatctgcgt tttcttctgc
     5161 actttttaca tgaggagcaa tcaatttatc aaattgcact gcttgttccg tgattgcttc
     5221 tgcctgactt ttttctttgc cggcaagctc taataactga acagccatat caaagaaaac
     5281 gcctattagt tgtgcaccgt tgggatggtc tgatgcataa tacgtcttat ctggaaggaa
     5341 taaagaaggc ggataagcaa agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac
     5401 atccagatta aatggcaatg gtaatgtatc caacagccat tctggaaatt gcttattcaa
     5461 ttcgtcgaaa gactgcaatg attcaacttt ttcgattata ggttgaagcg gttgagcatg
     5521 caactcattt cttcggtcat aatcgtttgc cagttgataa tatgcaagga aatgtttcat
     5581 tttttctgaa gacacttttt ctggatgggc aagcagatcg tctgtttctc ccatcaaaag
     5641 ttggtcgatc ccgtcaacta aatcttggaa accgcctgta gctggtttat ctgcagggat
     5701 ttttgctgtt ttttgccatt cttcattgac tgcttcatag aaatcttgtt tcaatcgttc
     5761 tttgttcatt catcgatcgc tccttttttg tttcgtgtgc ccattatctt acaaaatgaa
     5821 ggaattaaca aatagaacat aaaaaattga aaattcgaac aatcaaactg atttgttttt
     5881 ttcaatcctt cacttataat gaaaaaaagg aggcgatccc aactgaatca agtagtggat
     5941 aaacctttat atttcaaagt agataaaaaa attaaaaaat tagccagcac tcagcagctg
     6001 cagtcaagaa aaagtaaatt gcttttttta gctctggttt ttgaagatca aagttatgtg
     6061 attatcgatc aatcaggaca tccggtagaa tattctccag caaaatatac ttatcaagag
     6121 ggtctttctt gtaaaaaatg gaagctgatc aatgaagagc caatcgagtt atctagatgg
     6181 atcaacagaa aagaagatat tcccgttttg atcgaagaaa aaaggtcagg aaaggaactc
     6241 gcaaattgct gggtgggatt gccagaagaa cgttttttga ggtataagaa atgggctacc
     6301 ccctcaggct atctttgtgg tacttacgcg gcagcagttc tccttgccta ttatcaagac
     6361 tatcgtaagg ggtggatgct tccaaatgag attcgcaaga aaaatacggc tgattcgttg
     6421 gttttgacga aggcattgcg tagccagatc caaccactag gacttcctac gattcctttt
     6481 caggttagta cagggatttc tagtttttta aagaagaatg gcaatcatga gcgtgcacga
     6541 gcaactcttc taggttcatg gcagcgagct acaaaacgta tacgtgaagg caaacctgtg
     6601 atgattggta ttttgaaagt acttggcagt acatatggca atcattgggt gacagcgtac
     6661 gcttattttg aaactgaaac aggagaacgt tattacaaag tacacgacaa ctggggcgat
     6721 tatcataaag tcattcctgc cagttggagt aacggaacag tttcacttcc ttaagaattt
     6781 gctgaaatta acagaaaatc ttggaaaaat atgttaaact atcgaagaat agagaagttt
     6841 tggggaggac tacatgagta ataagtatca ggatgacacg ttgaagattt ttagtttgaa
     6901 tggcaaccgt ccattagctg aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag
     6961 cgtcgtgaag caatttagtg atggcgagat ttccatcaat atcgaagaaa gtattcgagg
     7021 ggatcatgtg tatatcgttc aatcaacgaa tgcacctgtc aatgattact atatggaact
     7081 actgatcatg atcgatgcaa tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc
     7141 ttactatggg tatgctaggc aagaccgcac ggcgaaacct catgaaccga tcactgcgaa
     7201 attgatcgct aacttgatcg aagaagcagg ggcaactcga gtactgactt tagatctgca
     7261 cacggttcaa gtacaaggat tctttgatat tcctgtagat aatttattta cgatgccgtt
     7321 gtttgctcat tattaccgtc aattaggtct gacgggagat gacatcgtag ttgtttcgcc
     7381 aaaaaacagc ggtgttcagc gtgcacgtag tctttcagaa tatttgaaca gcacattagc
     7441 catcgtagat catgcagatg atgcttcaac tgagggtggt tatgtgatcg gtgatgttca
     7501 aggaaagaca tgtatcatgg tagatgatat cttgaacaca ggagcaacgc ttgcacgagc
     7561 agcaaatgta ttaaaagaaa acggcgcaaa agaagtatat gcctgtgcgt cacatggatt
     7621 attgtccgat catgcgaaaa gtattttaga caatgcacca atcaaagata tttgtatcac
     7681 tgattctgtt ttcacagaag aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc
     7741 agaattaatg ggtgaagcgg tcaaacgcat ccatgaaaat acaccgatga gtccgttatt
     7801 ccgtctggaa gaaaaagaat tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga
     7861 agtgagcagc ttcaaggagc tgcgacaaga cttttgtcac agctcctttt ttctatgccc
     7921 atgaaacgtc ctctagacaa aaatgagctc ttctttggtt tgttcgctat tttatgctag
     7981 aatgtgccta gcaataattt ggaagggatg aatgaagtga aaactattta tctggatcat
     8041 gcggcaacta cccccatgca tccacaagta attgaagcga tgacggcaat catgcaagac
     8101 acgtttggaa atccttcaag catacatgga tttggtcgcc acgcgcatga aaaattggaa
     8161 acagcaagac aaatcattgc agacagtctg caagcaaaac ctcacgagat catctttaac
     8221 agtgggggaa cagaagggga taatacagct atcttagaaa cagcgttctc gcgtcaaaaa
     8281 gaaggcagac atctaatcac tacagctatc gaacatcctg ctgttcttaa taccatgaaa
     8341 tatttagaga caaaggggtt tgacgttaca tatctgcctg tagatgaaaa agggaatctg
     8401 tcgatcgaag aggtcaaaaa agcgttacga gaagatacga tcttggtctc tgtgatgtac
     8461 gggaacaatg aaatcggaaa tttgatgcca atcaaagaaa tcggtgaact tttagcggat
     8521 catccagctt atttccatac agatgcggta caagcgtacg gaaaccaaac gatccatcca
     8581 gaagaattag gaatcgattt attaagtatc tctgctcata aaatcaatgg gccaaaaggc
     8641 gtcggtttct tatataaacg agacggagta aatatccctg cattgctttt aggcggggag
     8701 caggaagaaa agcggcgtgc aggtacagaa aatttagcag ggatctgggg tatggcaaaa
     8761 gcagttgaac tgctgtcacc tgaagaacgt gaagcacgta acaacaaata taaaggattc
     8821 cagcaattga ttcttgatac attagaagca aacgaaatcc cttatcaagt aaatggtgat
     8881 ttaacgaata aactccctca tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg
     8941 ctactgatga agcttgatct taatggggtc gctatttcta ctggttcagc ttgtacagca
     9001 ggaaatatcg agccttcaca cgtattagaa gcaatgtatg gagaagatac accgatacta
     9061 aaagaatcca tccgtatcag ctttggtttg ggaaataccg aagacgacat ccagtatttc
     9121 cttgatcaat tagttcgtgc tgtcaaaaaa taactagtga agtcattctt atatgaacag
     9181 atgtctgaga gaacgccttc attttttagg gaacttaact gaaaaaccta gtaaaaatca
     9241 ggataaactt ttataataaa gagaaactaa ttttatcgag gtgaaaatca tggcatttga
     9301 aacaacagca acggtactgg gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag
     9361 atatacattg cgcgataatg gatttacaga aacaaacgga ggcaattatc aattgatccg
     9421 accattagat gccacgccgc aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa
     9481 agatattcaa acattgaaaa tgtcgatcac tacagcaaat ggattgaaag cagtcaatat
     9541 tttcaaagat ccaaaacaga aaatgagcca agataaattt tattttctga tggatgggat
     9601 gattagccgt gggttattcg aaaaagtcga atgatctgtg gatataaaaa gacaactagt
     9661 tttgaaacgg cctattgtta gatgactaac tttgtaagga cgacgcaaat tagttgtctt
     9721 ttttattttt aaatttaaca catgttgtta tttttaatat tttgctgatt tattgtttct
     9781 aagtaaagaa atttaaattg aaacgattgt tatttaggga aaaaggtatt ttttgaaaaa
     9841 agaatcccgt ataatgattg ttgtgcaaac gctatacaaa atgaaaaaga aaagagggat
     9901 gtttatggaa aatgaacagt cggtcgtttt aacgaattgg aagagaaatt atttgttctt
     9961 tttatcggga cagtttttgt caggaatcac tagtatggtc gtccaatatg cgatcatctg
    10021 gtatttgaca agagaaaccg gttcggcgac aattttgagt tttgctactt tactaggaat
    10081 gattcccatg gttttactaa gcccatttgt aggaccgcta gtggatcgct gggataaaaa
    10141 agctcttttg attgtcacgg atattattgt agcgattttc gcgcttattt tagcagtagt
    10201 cggaacgatt tcagaatcct tcccgatttg gctagttttt gtgtctttgt ttatgcgttc
    10261 ggtcgcacaa acattccaaa tgccaacgat ccagtcgatt atgccgacaa tcgttccatc
    10321 ttctcacata acaaggacga atggacagtt aggaatggtc caatcagcga atttcatcat
    10381 tgcgccagct ctaggtgcgg ctttgttttc agtcgttcct gtcaattacc ttattttatt
    10441 agatgtattg ggggcagttt ttggtgtcgg cttattgatt tttgtgaaga tcccaaaagt
    10501 ttctcctgaa atattggaag ttccgcttac tatttttaaa gatgcaaaat ttgggctgca
    10561 gcaattgatg gataataaag ggttatggta cataacgatc aatggtgcat ttgtgatgct
    10621 gttgttcatg ccagcaatca gtctgtatcc attgatgacg ctagattact ttggtggttc
    10681 cgttggacag gcgggagcag tggaagtagt ctacgcagtc ggaatgctct taggcggtgc
    10741 actgatcagt tttataggaa catggaaaga ccgaatgaaa cctattatta tagcctacat
    10801 cattatgggc ctgacgatcg gtgcaagcgg attggttcca aacgatagtc aagggttctt
    10861 gtactttctt atcttgaatg caggagcagg ttgtgcaacg ccatatttca atacactgct
    10921 gatggcaatg atccagcaga gttatgaatc gaatgtttta ggtcgtgtct tagggaactt
    10981 caactctttg atgaacctgg caggtccaat tggattatta tttgcaggac cattagctga
    11041 tcgtttaggg gtagaaaaaa tgttcttgtt ttccggaatc gggattttat tatgcggaat
    11101 cgttttattt ttaacgtcag ctgcccgaaa ttatgataaa gaattacaga aaaaactggt
    11161 aaaagaacac catgagcaaa aagacgaata gtatggaatg gagttgtgac agaagtcttg
    11221 ttacaactcc atttctgatc ctaatgttcc tgtggcaata agcctactca aatgaaaaat
    11281 atgagtaatt atctttattt gtttaatact tgatataggg cagttttttc acgacctatc
    11341 gtacttcctc tttgcttcat agatttcttt gatttgttgc tcagataacg gagtgccaaa
    11401 tggtagtttt tcctttaaga gttctctttc cggtgcgtct acgcctacat tgacatcgtt
    11461 ctcgataggt acgctgtaat gatggatatg tccatgaaga ttgcggatag tgtcgttagg
    11521 cccgagcaac atcggataat gagtcaagta atattgttga tggtcgaatt taacgatggc
    11581 acctacatca taaaaataaa atttgggact tccgtcagta agcctaggat catttttttc
    11641 taaataacgg aaaaaagcgc gactgtcatg gttccctttg atcaagtgga ttgttccttg
    11701 cagttgagtg accagttcca aaatttctgc atttgctttt tcatattgcg gatgcatagc
    11761 aaggtcgcct agatgataaa catggtcggt ttcttttatt actgcattcc agctagtgat
    11821 caactgttgg ttcatttcat ccacatttgc aaagagccgc ggagcaaagt cgttttttcc
    11881 taataagcgt tcatggaaaa agtgcatatc actgataaag taattcatgt atcttttgcc
    11941 tccctttttt cttagagtaa acaaaatcac ttatctgagc aactcataac atctaaaaag
    12001 aaaaggttgt aaatgagacg tgcagcttta agcgataaga agcaattttg aaaaatagtt
    12061 tttcacaatt tataaaatta ttccttattg ccgaaaggct ggcccttttt atgagtatgc
    12121 caatttttta tatcgaatgc tcaaaagatg agtgattttt tggttgacta tcatgtttgt
    12181 gtactgaata ggtccagtag aacagtattt ttacgacttg ataattaagg gatgaaattt
    12241 tcagaaatat gacgtataat gtaaaatact gaaaagttgc gaccttcaac tcgtagatgt
    12301 ttcagaatct aattgaaatg atggtgatat catgacagac aacagcaaaa cacgcgttgt
    12361 cgtagggatg agcggcggtg tcgactcatc tgtaacagca cttttgttaa aggaacaagg
    12421 atacgacgtg atcggaatat tcatgaaaaa ttgggatgac acggacgaaa acggtgtatg
    12481 cacggccacg gaagattata aggatgtagc gaaagtagct gctcaaatcg ggattcctta
    12541 ttactctgtg aattttgaaa aagaatattg ggatcgtgta tttgaatatt tcctagccga
    12601 ataccgcgca ggacgtacac ctaacccaga cgtgatgtgc aacaaagaaa tcaaattcaa
    12661 agcattcttg gattatgcaa tggatcttgg cgccgattat gtagctactg gacattatgc
    12721 gcaagtcact cgcgatgaaa acggcgtggt ccacatgctt cgtggagtag acaataataa
    12781 agatcaaact tacttcctta gccaattatc acaagaacag ctttcaaaaa cgatgttccc
    12841 tctaggcggc atggaaaaat cagaagtacg ggcaattgct gaacgcgcag gccttgcgac
    12901 tgctaagaaa aaagattcaa cgggaatctg ctttattggt gaaaagaact tcaaacaatt
    12961 tttgagcaac tatctgccag caaaaaaagg aaatatggtc actcttgatg gtgaagtcaa
    13021 aggacaacat gctggcttga tgtattatac gattggtcaa cgtcaaggac tagggatcgg
    13081 tggcggagga gattcgcaag aaccctggtt tgttgtagga aaagatctag cgacaaacac
    13141 attatatgtg gggcaagggt tccatcatcc tgcattgtat gccacaagct tggatgcaag
    13201 tgaaatccac tttacgacga atgaaccaat gccaaaagaa ttcaagtgta cagcgaaatt
    13261 ccgttatcgt cagcaagacg ttcctgtgac agttcgctta ttggatgata atcgtgcaga
    13321 agtcgtattc gacgaacctg tacgtgcaat cactcctgga caagcagttg tattttatga
    13381 tggaatggaa tgtctgggtg gtggtttgat cgaccacgca tatcaagaaa caaaagtttt
    13441 acaatacgtt taagcgatat taaatgaatc aaaacagcta tggctaacaa aagaaacacc
    13501 ttttcttttg gatagcatag ctgttttttt gcaataaaat taatttttat tcattttata
    13561 cttgaaatac ttgtgtaaaa agttcataat atccaataat atgaataaag ttgctttttg
    13621 ttaagcaaga gcagttgcaa aatagaaggg aatcttttgc aagtgatttg ttcaatttta
    13681 taagtgaggg tgatggaaat ggaaaagaaa tcgacgggta ttagcaaatg gggactggtt
    13741 gctctggttg tcagttcgtc aatcggaagc ggtgtgtttg ggatcacaag tgatctggct
    13801 agttctgcag ctccagggcc agcgatcctt tcatggataa tcgttgggat tggtatttta
    13861 gctttggtat tatcgctgaa tcatttagga gaaaagcgtc cagatctaga tggtggtatt
    13921 ttcggttacg cggaagcttc atttggtaaa ttaggtggct tcatcagtgg ttggggttac
    13981 tggttgtctg cgtggctggg aaatgttgct tttgcaacga tgctgatgag cgccatggga
    14041 gaatttttcc cgatattcaa aggtggacaa aatgttcctt ctattttatt tgcaagtatt
    14101 tttatttggg gtctgacttt attagtaaac aatggagttg aaggagcgag cgtcatcaat
    14161 acaatcgtga cgatctgtaa attagtgcca ttattcctat ttttaatatt tgtatttatc
    14221 gcatttaaag tgaatatatt cactgctgat ttttggggta acgtatctga taatctagtg
    14281 aatggaacag ggcagcaagg aacgatctgg ttgcagatta aaggttgttt gatggttatg
    14341 atgtgggtct ttgtagggat cgaaggcgca agtgttttag ccaaccgcgc gaagaaacgt
    14401 tcagaagccc agcaagcatc gatcattggt ctgctttgtt tattgtttat ctatatcctt
    14461 gcttcgatct tgccatacgg cgtcttatct caagaagagt tagcaactat ttcacaacct
    14521 gctatggcaa atattttaaa aggaatcgtg ggaaattggg gagcagcgct gatcaatatc
    14581 ggcttgatta tttcgatcat tggttcttgg ttgtcttgga cgatgctccc agcagaaaca
    14641 acgatgttga tggctcgaga cggtctgttg ccgaaaggtt ggggaaaaac gaataaaaaa
    14701 aatgccccaa cttattcgtt agttgttact gcaatcttga ctaatctatt cttattgact
    14761 ttcttagtta ctgattatgc ctatcaattt gcctattctt tatgtacagc ggcaattttg
    14821 atttgttatc tacttgtcgg gatctatcaa gtccagtttt ctttaaaaga aggaatcaaa
    14881 aaacaagtag tgatcggtgg gattgctgta gtttttgaat tgatgggcat cattttagca
    14941 ggattttctt atgtcctttt atgtagtatt gcctatcttc ctggtttttg cttctactgg
    15001 atcgcttgtc gtgaaaccaa acatcaaatt acgggaaaag aaaaagtaat gattgggctg
    15061 atttgtgtgg ccgcactgct ttcaatcggg ttgcttgctg taggctggat caagatataa
    15121 gggggaaaga aacatgaaaa tcgcaaattt cggtgtagaa gaatggctaa ataaatggga
    15181 aaagaaagcg gtatatgata ttgcacaaag ctcgatcgaa gcattgacat tagacgaact
    15241 cgttggctta gatgggacta gcgtaagtaa atttttcgaa aaacgcaaaa acgaaccatt
    15301 ggactatggc tggatcgaag gatcagatgt tttcaaacaa gaagtcgctt ctctttatca
    15361 aaccgtcgat ccggaaaaca tcttacaaac gaatggagcg actggtgcta atcttttagc
    15421 tctatacgcg ttagtggaag caggagacca tgttgtttcg atgcttccta cttatcagca
    15481 attatatgac atccccaaat ctttaggtgc cgctgttgac tttgttcatt tgaaggaaga
    15541 agaagattgg cagtttgatt tagaacagct agaagcctta gtcaaacctg aaacaaaaat
    15601 catctgtttg aatagtgcaa acaatcctac aggcacattg ctagatcgga aaactttaga
    15661 aaaaatcgca gaaattgctc gtacagtaga tgcatatgta ttgatagatg aagtatatgc
    15721 gcctttaacg gataaaggtg aattcttgtc gatcgtagat gtctatgaaa aaggtatagc
    15781 aaccaattcc ctatccaaaa cctactccat tccaggtatt cgtattggct ggacagctac
    15841 tggacctgag ttggcagaag tttttcgaaa gtatcgagac tacacgatga tttgcggcgg
    15901 agtcctgtca gatgatcttg ctgtacatgc attgaagaat aaagaaaaaa tattggcgcg
    15961 aaacaaaaag atcattacag agaaccttgt gattttaaaa caatgggtag ccaatgaacc
    16021 gaaagttgaa cttgtcgctc ccaattacgt ctctacttct ttcattaaat tagccatcga
    16081 agaagatgac cagacatttt gtatcaattt attagaagaa acaggagtac tattagtacc
    16141 aggaagtgcc tttgatttac cgaagcatgc ccgactaggt tactgttgta aaaaagaaac
    16201 cttggaaaag ggattattcc tcttatctga gttcttgaaa aaacaggcct aaacaaaaac
    16261 actgcaagga cagtctgtga caactgttca agcagtgttt ttcatttacg cttattgtct
    16321 atgagaagga gagtgccctg aagctatgga gactcctgaa gctttttctt ctgcagtttc
    16381 ttgctgatgg atctcttgtg tattgtgtcg tacaagcaag aagttgacca aaacaagaag
    16441 agaagtcgaa agaaagacgc cgcgataacc aaatgcactg gaaacactgg agccgatcat
    16501 aggccctact acgttaccag ttgcttggaa agattggtta taactgaaga tccgtccagc
    16561 agcttgatga ggcgaatatt ttgtgatcaa tgcttggaca gcagggagca gacaagcatc
    16621 agagatacca atcaaaaaac gtaaggcagc taattgccaa acattcgtga cgaatgccat
    16681 tgggatataa accagaatcg caaaacccaa accaatcgca agtattcttt cactcccaat
    16741 cttgtcacct aagcgaccaa atcttggagc ggctatcaaa gtggcaatcc caggtatcga
    16801 agcaatgatt ccgctgatca acgtaacatt cccatgtcca tgcagcaatt ggcgaatgta
    16861 cagactgatg attggactaa tggaattgtt ggatgcttgg atgatcatcg ttgtgacgaa
    16921 cataccgatg acgacgtgtg gatatttcaa gtctttgaaa atctgtttgg cagaaaccat
    16981 gttttctttt ttcactggga caaatttttc atggacgaaa aacagactca acaagaagac
    17041 gaaaaataag atgatccctg taatgaaaaa ggtagggcga tagccaaacg ctgaagcaga
    17101 gatcccgcca agaagcggtc caagtaatgt tcctgtaaca gaaccagttg ctaaggttcc
    17161 aagaacttga ccactttttt cttttggcgt cccagttgcg acaagggcag tggcattact
    17221 gatataacca gaaaaaatcc cttgaagcag acgcaatgcg actaattgat acacactagt
    17281 cacgcaaccc atcaaagaaa tgacgattgc cattcctaat gaagcacgca acagcatcaa
    17341 ttttcttcct ttttggtcgg ctaattttcc ccaccaagga gagataagcg tagtcaccaa
    17401 gaaagtagat gaaaaagtca atccgctcca aaagttcaat tgtgacgtac tataattacc
    17461 taacgtatct atgtaaagtg acataaaagg catgactaaa ctaaatccga taccagccat
    17521 aaatgttcca aaccataaga caaacaagtt ttgttcccaa gtttctcttt tcctaaataa
    17581 tttttccttt attcttgcag ccaattagac tccttctttc tgaaaatttt catctacagc
    17641 tctttattat aaactacaat atgtagttta tcaatgaaag tctctttttt ctctttcttt
    17701 ttcgtggtaa actgaaagag tgaaggattt attttatgag aggaaacatg gaaatgaaaa
    17761 aaagaaatct gtctcaagag aaaatcatcg actgctttcg agaactagca gaagagatgg
    17821 atgttcagca aataaccttt cagcatttag ccaaacgctt ggatatcaag gctccctcgc
    17881 tgtataacca tttcaaaaat attcgtgatg tcaaaacagc gctaacagcg aaacttctgc
    17941 aggaacttaa tgaccagctg cgacgggctc ttgtgggaaa aagtggtcag gaagcactgc
    18001 agatctatgc agaaacttat cgatcctttg ctttttcaaa tcaagcagtg tatgagttac
    18061 tgatcagtgt accgcatacc aatgaagctg ttttattaga tggaatccat gaaacgaatc
    18121 agatcatttt gcagatattt gaggcttttc aattaacgaa agaagaaaaa gttcatcgaa
    18181 gcagggaact tcgtagtatg atccacggtt atctttcctt acgtttttta ggatacttta
    18241 caaaagaacc aacaatcgat ccagaagaca gctatttttg gatgatcaat aactttatcg
    18301 ctactttgcc ttaaacaatc agattcattt gtaacaattt taagatgttc attaaatgac
    18361 ccttttataa gagaaaattc ttgcaacttt cttgaaaagt gactaagatg agtctagaaa
    18421 agctgaaaga aggagcacga gtatgtatca agtaattacc atgttcgggg acaatgagcc
    18481 ttggtggttt tttgaagact gggaagaaga tattgaagaa gaagaaacat tcgagtcttt
    18541 cgaagaagca cgacaagcgt acgaaaaaca atggaacgaa atccatcaaa attatgaata
    18601 tatcaatgca aaaagtaact ttctcagcgc cttttggaat gacggagatg agcgctggtg
    18661 tgaagagtgt gacgatgatt tacagcaata caagggactt gcgttgttga aaaatcatca
    18721 gcccatcaca gtagaaagca gaaaggaatt ttatgaaaca actaattcta gcggaaaaac
    18781 caagcgttgc gaaagaccta agcaaggtgc ttggtgctaa tcaaaagcac aaaaattatt
    18841 atgaaggacc caaggtgatc gttacttggg cccttggtca tttattagga ttgaaaatgc
    18901 cggaagatct gaataaggaa tggcagagct ggcaaatgga cacgttgcca atgatcccta
    18961 aaaaactagg aatcaaacca cttcctaaaa caggacatca attaaaagca atcaagcagc
    19021 tggctcagcg aaaagatgtc tcagaagcag tcattgcgac agatgccgga agagaagggg
    19081 aacttgttgc tcgctggatc ttagagtatg tgcatttcaa taaaccagtg aagcggttat
    19141 ggatctcttc tcaaacagat aaagcaatca aaactggatt caagcaacta agacctgcga
    19201 aagaatatga tgcattgtat gaatctgctc ttgcccgggc aaaagcggac tggctggtag
    19261 gattgaatgt gaccagagcc ttaactgtga agtacgcgga taatttatca gcaggccgtg
    19321 tgcagacacc gacactagca ttagtgagag accaagaaaa gaaaatcgag cagttccgtc
    19381 cacaaacgta tttcacgatc acgttgcaag ctggaaaaga acaagccaaa atgcttcaaa
    19441 aaaatcaatt tgctttaaaa acacaagaag aagcacaaaa acttgttcaa ttgatcagta
    19501 caagcaaagg aactgtcaca gaagtccaag aaaaattaaa aacggaatct gcgccattgc
    19561 cttatgattt aacagagatc caaagagaag ccaatcaacg ctatggctat tctgcaaaga
    19621 aaacattaag tcttgtacaa agtttgtatg agacccataa gatcgttact tatccgcgta
    19681 cagacagcaa atatctgaca aatgacatga aaagtacgat gaaagaacga ttgcaagcag
    19741 tcagcgactt tgctcctgag gtcaagcagt atttgaaaaa cggagccatc gtccaacaga
    19801 caaaagtatt ccaagacagt aaagtgaccg atcaccatgc acttttgcca actgaaaaca
    19861 gagcacgtta tgaaaaatta agtaatgaag agcgaaaaat ctatcaaatg attgtctctc
    19921 gttttttagg actgtttgct caaccccata aagtcagcca aacaaaagtt acggttgaat
    19981 ttggtaaaga acgattcatt tttcggcaaa atcgtgtcgt ccaagcagga tggaaaggcg
    20041 aaactgaaac tgagacggaa acagtcaaat gggaaaaagg aatgagaatc aatcccgatt
    20101 tcgccatcaa aaaagaacta tctgcaccgc ctaaaccatt gtcagaagca agcttgcttg
    20161 gtcaaatgga aaaatacagt ctgggaaccc cagctacgcg tgcagagatt atcgagaaac
    20221 taatcaaatc tgaactgatg gaacgtaccc ctcaaggact gcaagtatct cctaaaggta
    20281 agcaattatt agtattggta aatccctctt tagtaacacc agatttgaca gaaagctggg
    20341 aaaaagacct tgaagctatt gctgcaggaa agaaacaagc aggaaccttc ttgaatggaa
    20401 tcgaaaaaga aacaaaacgc ttggtgaatg agatcaaatc aagcaaacaa gaatatcaag
    20461 atttctcgat tacccaaaag aaatgcccag aatgtggggc caatctgcgc gagaaaaaca
    20521 cacgtgacgg caaaatctat gtctgcacca attcagattg caactaccgc agaagaaaag
    20581 atccaaaagt ctctaatcat cgatgcccgc aatgtcaccg gaaaatggaa atcatcgaag
    20641 gtaaaaacgg tgcttatttc cgttgtaaat acgatggaac gacagaaaaa ttgcttgata
    20701 aaaaagagca gaagaagaaa atgaccaaac atgaagaacg acgcttgctg aaaaaatatg
    20761 cacaagcaga agaaccagaa gaaagcccat tagcagcagc actaaaagca gcaatgaaga
    20821 agtaataaga tatgaaagag gttgtgatag aagtggtcag cttcaagaaa taagaaggaa
    20881 tttacgaaaa ttgcttctca aatttttgtg aaatttcggc ttatttccga agaagctact
    20941 tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca cagtcctttt
    21001 tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt caattcattt
    21061 ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt ttctaagaag
    21121 attttcccaa tatatttccc gatgattcca agacataaca attggattcc accaatgaac
    21181 aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa caatgcccgc
    21241 aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc gataaatgaa
    21301 gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta gttgaataat
    21361 ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata agaaatatat
    21421 tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta ttctgttaat
    21481 tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc gccatcgacc
    21541 atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa gaaactgcgt
    21601 atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat atcagaagta
    21661 gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc aacatccatc
    21721 acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc ttctttccca
    21781 aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag cttcttgatt
    21841 gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa ctcatgggca
    21901 cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc attaaaacat
    21961 gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt atatatggtt
    22021 tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt acgtaaaaga
    22081 acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg gtttatcaaa
    22141 cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa aggaggaagt
    22201 tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg gctttttatg
    22261 gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt cagaaagcga
    22321 atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat attttgcggg
    22381 aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt ttcagcaaaa
    22441 tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata aagaagtagc
    22501 cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc gaaccaatcc
    22561 attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac tgacaggata
    22621 tatgggaaac gcaatagatg gggttgaaat caaacgccaa tt
//
LOCUS       DAYYCQ010000045        21814 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000045 DAYYCQ010000000
VERSION     DAYYCQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.045"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(531..2027)
                     /gene="lysS"
                     /locus_tag="ACHAYF_001691"
     CDS             complement(531..2027)
                     /gene="lysS"
                     /locus_tag="ACHAYF_001691"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8579132.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="ACHAYF_001692"
     CDS             complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="ACHAYF_001692"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8579133.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="ACHAYF_001693"
     CDS             complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="ACHAYF_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8579134.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4247..6358)
                     /gene="ftsH"
                     /locus_tag="ACHAYF_001694"
     CDS             complement(4247..6358)
                     /gene="ftsH"
                     /locus_tag="ACHAYF_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8579135.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="ACHAYF_001695"
     CDS             complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="ACHAYF_001695"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8579136.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="ACHAYF_001696"
     CDS             complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="ACHAYF_001696"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8579137.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8558..9028)
                     /locus_tag="ACHAYF_001697"
     CDS             complement(8558..9028)
                     /locus_tag="ACHAYF_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8579138.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9074..9499)
                     /locus_tag="ACHAYF_001698"
     CDS             complement(9074..9499)
                     /locus_tag="ACHAYF_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8579139.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9566..9835)
                     /locus_tag="ACHAYF_001699"
     CDS             complement(9566..9835)
                     /locus_tag="ACHAYF_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8579140.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9846..11441)
                     /locus_tag="ACHAYF_001700"
     CDS             complement(9846..11441)
                     /locus_tag="ACHAYF_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8579141.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="ACHAYF_001701"
     CDS             complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="ACHAYF_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8579142.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14994..15554)
                     /gene="pth"
                     /locus_tag="ACHAYF_001702"
     CDS             complement(14994..15554)
                     /gene="pth"
                     /locus_tag="ACHAYF_001702"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8579143.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15907..16881
                     /locus_tag="ACHAYF_001703"
     CDS             15907..16881
                     /locus_tag="ACHAYF_001703"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8579144.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17051..18451
                     /locus_tag="ACHAYF_001704"
     CDS             17051..18451
                     /locus_tag="ACHAYF_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8579145.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18529..19251)
                     /locus_tag="ACHAYF_001705"
     CDS             complement(18529..19251)
                     /locus_tag="ACHAYF_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8579146.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19330..19836)
                     /locus_tag="ACHAYF_001706"
     CDS             complement(19330..19836)
                     /locus_tag="ACHAYF_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8579147.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19959..21758
                     /locus_tag="ACHAYF_001707"
     CDS             19959..21758
                     /locus_tag="ACHAYF_001707"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8579148.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6054 a         4752 c         3911 g         7097 t
ORIGIN      
        1 agagtgtttc ccgattcatt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcgg
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg
      241 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
      301 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      361 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      421 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      481 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      541 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      601 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      661 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      721 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      781 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      841 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      901 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      961 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
     1021 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
     1081 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
     1141 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
     1201 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1261 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1321 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1381 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1441 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1501 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1561 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1621 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1681 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1741 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1801 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1861 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1921 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1981 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     2041 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     2101 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     2161 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     2221 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2281 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2341 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttcccctg
     2401 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2461 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2521 catccccgtt ccccataaat ggaatcgtca aatgtttttt cacttctttc aatacttccc
     2581 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2641 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2701 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2761 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2821 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2881 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2941 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     3001 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     3061 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     3121 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     3181 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3241 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3301 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3361 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3421 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3481 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3541 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3601 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3661 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3721 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3781 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3841 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3901 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3961 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     4021 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     4081 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     4141 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     4201 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4261 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4321 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4381 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4441 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4501 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4561 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4621 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4681 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4741 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4801 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4861 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4921 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4981 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     5041 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     5101 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     5161 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     5221 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5281 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5341 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5401 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5461 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5521 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5581 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5641 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5701 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5761 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5821 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5881 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5941 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     6001 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     6061 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     6121 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     6181 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6241 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6301 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6361 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6421 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6481 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6541 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6601 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6661 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6721 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6781 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6841 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6901 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6961 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     7021 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     7081 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     7141 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     7201 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7261 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7321 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7381 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7441 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7501 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7561 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7621 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7681 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7741 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7801 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7861 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7921 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7981 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     8041 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     8101 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     8161 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     8221 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8281 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8341 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8401 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8461 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8521 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8581 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8641 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8701 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8761 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8821 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8881 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8941 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     9001 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     9061 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     9121 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     9181 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9241 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9301 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9361 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9421 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9481 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9541 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9601 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9661 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9721 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9781 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9841 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9901 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9961 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
    10021 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
    10081 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
    10141 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
    10201 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10261 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10321 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10381 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10441 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10501 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10561 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10621 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10681 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10741 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10801 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10861 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10921 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10981 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    11041 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    11101 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    11161 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    11221 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11281 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11341 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11401 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11461 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11521 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11581 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11641 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11701 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11761 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11821 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11881 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11941 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    12001 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    12061 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    12121 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    12181 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12241 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12301 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12361 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12421 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12481 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12541 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12601 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12661 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12721 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12781 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12841 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12901 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12961 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    13021 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    13081 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    13141 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    13201 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13261 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13321 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13381 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13441 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13501 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13561 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13621 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13681 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13741 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13801 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13861 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13921 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13981 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    14041 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    14101 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    14161 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    14221 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14281 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14341 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14401 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14461 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14521 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14581 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14641 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14701 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14761 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14821 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14881 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14941 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    15001 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    15061 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    15121 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    15181 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15241 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15301 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15361 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15421 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15481 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15541 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15601 gctaattagc ggtccagtcc atcgagcttt tttctttttt acacttgtca atgcatagta
    15661 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa
    15721 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15781 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15841 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15901 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15961 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    16021 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    16081 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    16141 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    16201 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16261 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16321 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16381 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16441 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16501 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16561 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16621 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16681 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16741 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16801 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16861 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16921 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16981 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    17041 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    17101 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    17161 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    17221 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17281 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17341 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17401 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17461 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17521 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17581 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17641 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17701 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17761 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17821 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17881 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17941 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    18001 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    18061 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    18121 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    18181 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18241 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18301 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18361 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18421 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18481 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18541 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18601 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18661 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18721 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18781 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18841 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18901 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18961 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    19021 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    19081 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    19141 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    19201 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19261 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19321 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19381 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19441 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19501 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19561 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19621 cagatggttg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19681 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19741 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19801 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19861 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19921 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19981 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    20041 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    20101 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    20161 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    20221 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20281 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20341 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20401 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20461 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20521 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20581 gagaaacact tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg
    20641 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20701 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20761 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20821 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20881 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20941 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    21001 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    21061 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    21121 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    21181 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga attgatcgta gccgatgcta
    21241 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21301 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21361 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21421 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21481 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21541 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21601 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21661 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21721 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21781 aaagagttag tccagaatgg gctaactctt tttg
//
LOCUS       DAYYCQ010000046        21711 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000046 DAYYCQ010000000
VERSION     DAYYCQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.046"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..542
                     /locus_tag="ACHAYF_001708"
     CDS             21..542
                     /locus_tag="ACHAYF_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8579149.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            639..860
                     /locus_tag="ACHAYF_001709"
     CDS             639..860
                     /locus_tag="ACHAYF_001709"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8579150.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1017..1181)
                     /locus_tag="ACHAYF_001710"
     CDS             complement(1017..1181)
                     /locus_tag="ACHAYF_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM8579151.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1284..1805
                     /locus_tag="ACHAYF_001711"
     CDS             1284..1805
                     /locus_tag="ACHAYF_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8579152.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1792..2232
                     /locus_tag="ACHAYF_001712"
                     /pseudo
     CDS             1792..2232
                     /locus_tag="ACHAYF_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2272..2430)
                     /locus_tag="ACHAYF_001713"
     CDS             complement(2272..2430)
                     /locus_tag="ACHAYF_001713"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM8579153.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2523..3284
                     /locus_tag="ACHAYF_001714"
     CDS             2523..3284
                     /locus_tag="ACHAYF_001714"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8579154.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3471..4823
                     /locus_tag="ACHAYF_001715"
                     /pseudo
     CDS             3471..4823
                     /locus_tag="ACHAYF_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4839..6059
                     /locus_tag="ACHAYF_001716"
     CDS             4839..6059
                     /locus_tag="ACHAYF_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8579155.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6094..6771
                     /locus_tag="ACHAYF_001717"
     CDS             6094..6771
                     /locus_tag="ACHAYF_001717"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8579156.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6768..7964
                     /locus_tag="ACHAYF_001718"
     CDS             6768..7964
                     /locus_tag="ACHAYF_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8579157.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7979..8302
                     /locus_tag="ACHAYF_001719"
     CDS             7979..8302
                     /locus_tag="ACHAYF_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8579158.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8306..8641
                     /locus_tag="ACHAYF_001720"
     CDS             8306..8641
                     /locus_tag="ACHAYF_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8579159.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8638..9033
                     /locus_tag="ACHAYF_001721"
     CDS             8638..9033
                     /locus_tag="ACHAYF_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8579160.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9030..9401
                     /locus_tag="ACHAYF_001722"
     CDS             9030..9401
                     /locus_tag="ACHAYF_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579161.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9417..9977
                     /locus_tag="ACHAYF_001723"
     CDS             9417..9977
                     /locus_tag="ACHAYF_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8579162.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10104..10415
                     /locus_tag="ACHAYF_001724"
     CDS             10104..10415
                     /locus_tag="ACHAYF_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579163.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10439..10630
                     /locus_tag="ACHAYF_001725"
     CDS             10439..10630
                     /locus_tag="ACHAYF_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579164.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10630..14475
                     /locus_tag="ACHAYF_001726"
     CDS             10630..14475
                     /locus_tag="ACHAYF_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8579165.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14476..15348
                     /locus_tag="ACHAYF_001727"
     CDS             14476..15348
                     /locus_tag="ACHAYF_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8579166.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15345..16394
                     /locus_tag="ACHAYF_001728"
     CDS             15345..16394
                     /locus_tag="ACHAYF_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579167.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16398..17222
                     /locus_tag="ACHAYF_001729"
     CDS             16398..17222
                     /locus_tag="ACHAYF_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579168.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17240..19372
                     /locus_tag="ACHAYF_001730"
     CDS             17240..19372
                     /locus_tag="ACHAYF_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8579169.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19389..19538
                     /locus_tag="ACHAYF_001731"
     CDS             19389..19538
                     /locus_tag="ACHAYF_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579170.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19535..19981
                     /locus_tag="ACHAYF_001732"
     CDS             19535..19981
                     /locus_tag="ACHAYF_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579171.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19983..20120
                     /locus_tag="ACHAYF_001733"
     CDS             19983..20120
                     /locus_tag="ACHAYF_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8579172.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20157..20450
                     /locus_tag="ACHAYF_001734"
     CDS             20157..20450
                     /locus_tag="ACHAYF_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579173.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20473..20670
                     /locus_tag="ACHAYF_001735"
     CDS             20473..20670
                     /locus_tag="ACHAYF_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8579174.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20681..21700
                     /locus_tag="ACHAYF_001736"
     CDS             20681..21700
                     /locus_tag="ACHAYF_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8579175.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
BASE COUNT         7542 a         3734 c         4823 g         5612 t
ORIGIN      
        1 tggattaagg aggaagagta atgcatatag gagataagat acataaattg agacggctta
       61 ataacgaaac aatgaacgac ttagttaaac aaaccggact gtcaaaatca tgggtatctg
      121 atattgagtc tggaaaaaag caacgcgctg actttaataa gatatgtgca atagccaaac
      181 attataaagt tagcttagaa tactttcagg atgattattt ggatagtgat gaacacgagg
      241 atattattcc tttgacagac gaaacaagag ttaaatcagt gaaatcgctt gaacaaagcc
      301 taaagtatct gaataacaaa caagaaccta accttctaga gatcaaacta aaagatactg
      361 actcagtacc agaggtttat tacaaaggtc agaagttaga gggattgata gatgtaagct
      421 atcactacca tacaacaact gatgtggtag ctggttcaac aaacgacatc aatatggagt
      481 acctaggtga agtaactaag tatccagata taaaaacaat cggacacaag agagatgtgt
      541 aaatgaaaga agctagacct agagacgaga tagacaaact atacaagacc aaacgatggc
      601 gagacctaag gcaagtagta atagctaggg acttcggcat gtgccaagag tgcaagcgtc
      661 gagggcggaa cacaaggggc acgatcatcc atcacatagt cgaggcgagg gaagacctgt
      721 cactgttctg gtccgtagat aaccttgaat gtatctgtgt agcttgtcac aacagagagc
      781 atccagagag atcaggcggg aagaagaaac caaaacctaa atcacatatc gttaaaatgt
      841 attcaactcc tgaaagataa gtttgcagcg aaatgaaggt agccccccta ctctaaaaga
      901 ttaaagagta aggcttgaga agaacggtgc tgtccttcct tcgtaaaaag accgcttttc
      961 aagttttttg gagaaaaagg aaaaagccga ccaatttaag ccggctttgg acgaagctat
     1021 ttcttagtcc atttgtttcc tttttgagaa gtaggaggta atcggtcgcc tggatcaatt
     1081 tttacttctc gtccgccttg gacatttcca ccacgaggtc ccacttcttt ataggttcct
     1141 tttggtttat tgtcttcgcc gggtttatag agttctccca taggaatccc tccctaaaaa
     1201 aatttcggca cagcactgcc gataacttaa ttataaggat tgtgataacg atttcaatat
     1261 atcttttgaa agaaggtgat attatgccgc aaccagcgaa gagtgcaaaa ttacaattat
     1321 taaacggaaa cccaaataag aagaataccg aagaactccg caagcgagcg gccgcagaag
     1381 acaaattaaa aatggctact gacaaaatca aaccgccgtt atggctagat tcgctaggaa
     1441 aggatacctt tgagtttatc gctgatgaat tgctgtctgt ggatttaatc agtaatccgg
     1501 acgtccatac aatggctctc tactccaatt ggtattcgca atacgtttct ttagaaaaac
     1561 agcttcgaaa actacaacga gagtacaagt tgaactatgc gcttgcgaaa aaggaggcag
     1621 aggcgagagg tgagccgttt aatgaaccta atgaattaat tggtaatccg ctctctcggc
     1681 agatggatac agcgtcgcgg aatctccgtt cttttggcgc tgatttagga ctatcaccag
     1741 cagccagagc taagttagct attaagatgg ctgatgatgg tggtgatgac gatgacgact
     1801 tctaatattt tggatatgtc ctacacagaa cgtgtggact attggcaaag ctatcttgag
     1861 gagcaagctt cttggggtgg ctttttaaaa tgtccatatc cggaattgtt aactacttgg
     1921 tatgcggaac gattaatcga tggaagcata ccagccagca aagaaaatat tcaagctgct
     1981 aaacggcata tgcgtgattt gcagcgccaa ggaacagatg attttccttg gatctttgac
     2041 gaagaaaaag gtcaccggcc tattagatat atcgaaaaaa aatgtaaacc aactgaaggc
     2101 gactttggtt cgtttgtttt gcaaccttgg cagcatttca taattggatc catgtacgga
     2161 tgggtacatc gtgatacagg agagcgtcgc ttccgcgagg ctcttatttt tgttggacgt
     2221 aaaaacggta agttttgccg tttaaaaatc gggcaaaatc ggtgaaaacc tttatttttg
     2281 gctttctttt agtaataatt cgatagcttt atcaagcaat ttagacatag gaaccatagt
     2341 ttcttccgac atttttttta atccttcgta taaatcttta tcaattgcgt tagaaattct
     2401 aattctgttt tttagtccgt atttgtccat gtgtgacact cctttttatt ttactataac
     2461 atccgattcc accgcttgca actaccatca attgatggta gtatataaaa gaggtgagta
     2521 gattgaaaaa atacattatt tacaaacact taaataaaat aaatggcaaa atttatatcg
     2581 gtgtcacaaa tgatattggc agaaggtgga ggagcggtgg gattgaatat aaaccaccaa
     2641 agaacgaaac ccaacaccac agaagttttt ggaatgcaat acaaaaatac gggtgggaaa
     2701 attttgatca tttgattatt gaagaagact taacgatgaa agaagctttt gagaaagaaa
     2761 agttttatat tgaactttat gactcaacta ataagaaaaa aggttataac atcgcaaaag
     2821 gcggaaatgg cggaataata tataaagttc accccaaagg aatgctaggg aaaaaacaat
     2881 ctaaagaatt ttcttcaaat cacagtaaat gggcaaagaa ccataagaat aattgtatga
     2941 caaacggtga tgttgtatgg ggtgttactc acgaacaccc aaaaggcatg ttgggaaagc
     3001 accacagcaa agaatcaatt atgaaaaaga aagcatatag tggtgaaaat gctgtaacct
     3061 caaaaccaat tgtcgcaatt gaacgggatg gaactaaaag agaatttcac agcgctaagc
     3121 tttgcatggc ttactattcg attagtacat gtgtttttta ccgactttta aaagatggcg
     3181 ccccttatgt gataaatcct aaagcgaact acagaaataa agaaaaaata ttagctatcg
     3241 aaggaattat gttcaaacat aaagaagata ccgaggtaag ttagtagatt gcgaaaggtt
     3301 actaactacc gtaacgcgta ggagttgaat aaatataatg ctcccaagag tgtccgacag
     3361 cagataattt gctgaaaatg tacgctaaac tgggccagaa ctgactgacc gatgaaaatg
     3421 agggtgacct ccagagtgcg agataaaaag ctcgcagata ataacaatcg aaaacatcac
     3481 taatttcagg cctttccaca tacatggtcg cttatgatga tgaacaaggc gccaacgttt
     3541 acgtattggc aaatgctcgt gatcaagcaa gcttgttttt tgataaggcc gcagaaatgg
     3601 tcaaacaatc gccggcgctc tttaagaaat ttggtaagcc taaacgatca agtattaatt
     3661 atgctcccgc cttttctaaa atggaaccac gcgcctcaga tagccggaaa ttggatgggc
     3721 taaacactca ctttggtatt tttgacgaga tccacgagtt tacgaattac aatctgatca
     3781 acgttatcaa gaaatcaaga ggaaccagaa aacagcctct gatagtttat atcacaactg
     3841 ctggatatgt attagatggt ccgttgatgt cttattttga gcaaggtgtg gactgtttgg
     3901 aacatttgga agatgacatc gatgaacgga ctttctatta tctggcaaaa cttgacagtg
     3961 cggaagaggc tgatgaccca agattatgga tcaaagccaa tccgaatatt tgtctaatga
     4021 attttgttgg catgctagat gactatgtta aggataaaaa agatccaaaa gaatatgctg
     4081 actggattac caagcaattt aacttgtttt ccgatatcga tgagctgtca tttgtcgata
     4141 tgcctaccat taaacgaaac aataaaacca tcgatattga aacgctcaaa ggtaagaagt
     4201 gtgtcggtgg ttttgacttg tccgaaacgg aagactttac cgcagccgtt ttagaatttc
     4261 cgcttgaaac aggcgaggta ttcattttgc aacacacatg gatcccacaa gctagatttg
     4321 atcgagataa caatcaagag cgtatcaaag cgtgggagaa ggtgggagat ctaacgatta
     4381 ttcctggtga ttacgtcaat tatgaatacg tcttaaattg gtttgtagaa aattcgaaaa
     4441 tctatgacat tgtaaaaatc aattatgaca aggccaaggc gctacgatta aataaagaac
     4501 tagaaaatgc aggatttgaa accaacgaga ttcggcaagg gtttctatca ttaggtgggc
     4561 caatgcaaaa cttcaaggaa atgctattgg acggtaaggt gattttcaac aattccaagc
     4621 tttaccgatg gtatctatcc aacgtcaagc tggtgatgga tcgcaactca aactggatgc
     4681 cgtctaagca gtccaagagt agaaaaatag atggttttgc agcaagtttg aacagccacg
     4741 ccgaagtgtt gaatatgttg gttaatcctg tcggaaccgg gaaagtaacc tattactcga
     4801 tttccgattt aatgaatatg taagaaaggt gtggaggaat gagtatttta gatcgtttgc
     4861 gttcttttgg ccgagcgaag ccgaaagcga gcaaacaaga gtattttttg aatgacccgg
     4921 gattgatacc gtatttagtc ggaaaagatg aaatatcaga agggattttt tccgtaatta
     4981 gccgtgtatc gaacgttttt gcgtctctcc ctctcaaaat gatagatgcg gagtttggcc
     5041 aaccggacga ctgtcctgca tacaacttgt tgagcgaagg ccctcgatat tttacaaagt
     5101 ttgatttttt ccgggacgtg gaagttttga gaaactacca agggaatgcg tatgtgcaga
     5161 ttttccgaaa tatcaatgga gaagtagcag atatggcgtt aataaaacct ggtgcttgcc
     5221 atccagtgat tgatatggat agcggggagc tttactacca agtaactgcg actgacaaag
     5281 gcagttataa gcaagttatt tatgtacatt acatggaaat gctccacttt aaacaaccga
     5341 ggtttggtgg cttggaaggt acagacccca caaaagtatt aacgaatacc ctcggatatg
     5401 atcgagaagt ccgaaaaatc tctttaagtc agcttaaagg aggtaatgaa gggctaaaag
     5461 ttaagtttgc tagcaatgtg gatgaagaag ctaaaaaagc tacagttaaa aacattgctg
     5521 atttttatcg acaaaacggt ggactacttg tggaagaaaa cggtgtagaa atcgaacgtt
     5581 tacaacgaga gctggtagac agcaagcttt tagatactga taaagtatct cgctccagaa
     5641 tcgcgatggt ctacaacgtg ccggaacatt tcattgggag taaccagtcg agttactctt
     5701 cacaggaaca gctcaatatg gagtttttga catacaatct agtaccgacc gttaatcaat
     5761 atgaagcgga actaaataag aaaacactat cgagaaccga aaaagcaaaa ggttatcgat
     5821 ataagtttaa tgtcgcaagt ttgctaagag ctgacactca ggcaaggggg caattctatc
     5881 aaatcatgcg acgaggcgga gcatactcag cgaatgatgt ccgaagattt gaggacttgc
     5941 aaccgataaa taaaaccggt atggatgatt atcatatttc cggagaccta tatccaatcg
     6001 atatggatcc aacattaaga aaaacaaact cgtctaaaag cgtagccgaa aacggttagg
     6061 cttttttagt ttgcaccgaa gggaggtgaa aggatgaaaa aagtgacgtt aagcggcgat
     6121 gtcgtggata acgataccgc gtggttatat gactggtttg ggattgattg tatctcacca
     6181 gggaaaattt ctgccgctct tacagaagca gcgggggatg aagtagaact tgatatctca
     6241 tctaacggtg gggatgtcct agcggcaagc gaaatatata ccgctatccg tgcctatccg
     6301 ggaaaggtat ccggaaatgt tgtgagtatt gcagcaagcg ctgcaagtgt aatcgcttgt
     6361 gcttgcgaac cgcttagaat ctcgccaaca gcacacatca tgattcacaa cgcatgggtg
     6421 accactagcg gcaacgccga ggaattaaaa gccaatgcgg aaatgttaag cagtgtggat
     6481 gagtctatcg ttaatgctta cgagatcaag acaggactcg accggaaaaa gcttgctgat
     6541 ttaatggcaa aagatacttg gttaaacgca caaacagcag tggcggaagg ttttgcggat
     6601 gaaattatgt ttgcagaagc accagtaacg gtactcaatg cctctcaacc ggttattcca
     6661 aaaaacgcgg taactaagtt gaaaaatcta atactcaaag cggaaacacc gcaagaagaa
     6721 acgctcttgc agaaaaaact aaaagcctta aatggaggga aaaacgaatg aatttagaac
     6781 aattaaaaaa tgcgtgggtc gaggcgggaa gtaaagtctc tgatttaaac gcgcaactca
     6841 atgcagcatt ggttgacgat gaaaaaacag aagaagatgt agtaagtttg caagcacaag
     6901 taaaagcagc acgggctaaa cgggacggat tgaaagagca agtggcaaat atggaagccg
     6961 aacaagtctt aaacgtcaaa aaagaaccat tagataaaaa agatgaaaac ttgaaaaaca
     7021 agtttatcaa agactttaaa gcgatggtca atggtgatcc tgctattatg gctactttga
     7081 catctaatac ggatgaatct ggtaatgcta tcggattgac tattcctgta gatgtgcaaa
     7141 cgactattca tactttggtt cgtcagtttg actctttgca agaatacgta aacgttgaaa
     7201 aagtgaccac tactagcggt tctcgggttt atgaaaaatg gtctgatatt aacccactga
     7261 ccgctttgga tactgaagac ggtgaaatcc cagcaaatga tgatcctgca ctttacttga
     7321 tcaaatactt gatcaaacgc tacgcaggta tttctacagt aactaacagc ttgctaaaag
     7381 atactgccga aaacattttg gcatggttgt ctaactggat cgcgaaaaaa gtagttgtta
     7441 ctcgcaatac aaaaatcttg gcagctattg atggaatcaa agcagcgcaa aagaaagatg
     7501 ttacagatgt tgatgggatt aaagatatca taaacgtcca acttgatcca gctatcgaag
     7561 ctacatctat gtttattaca aaccaagatg gcttcaatgt tttagataaa gtgaaacgtg
     7621 ctgatggatc ttacttgtta caaaaagacg tagcttctgc aactggatat accttcttag
     7681 gtaaaccaat caagaaaatt gcttctcgtt tcttgccaaa caaaggtacg caagctactc
     7741 ctaaatatcc actgtatatt ggtgatctga aagaagctgt tacattgtat gatcgcgaaa
     7801 acatgagctt gctgacaacg aatattggcg ggggggcttt tgaaacagac accaccaaag
     7861 tacgggttat cgatcgattt gatgtgcagt tagtcgatga tgaagcagtt gttttagcta
     7921 catttacatc tatcgcaaac gaagtgccag taacaaacgg ttaaggagct gatttcttat
     7981 gattcttgat cctaaaatgg atttagacga aatcaaaaac gcactaaaga ttgataccga
     8041 tgatgacgat gtggaagtaa gacgtgcggt gcaagctgca attgcataca ttagaggggc
     8101 tatcgggaat gataagccct ctttttataa gcaagaaaat gacacggttg atctgattaa
     8161 tttagctatt ctgcaattag cagatcatta ttacaacgct cgatcagcaa ccgtgagtgg
     8221 gaacttgcga gagtacgatt taggttttac aagcctaatc ttgcaactca aagcaagata
     8281 tttgcttttt gtggaggagt agcgtatgcc ccttattcaa acaggaaatt taaatcaacg
     8341 catcaagttt gtccgagata cgacggtcaa ggacgaggac gggcaagttg tccggacttc
     8401 tacaaccatt cttacttgct gggcaagtgt gcagacacaa cgcctgaacg atattaagac
     8461 gtccattggt acggctttgg aaggaacact gacgttcatt atccgctatc aacaaaaatc
     8521 agagctaacc aatgatatga aagtgcgttg gaatggaaaa acgtttgaaa tcattacgat
     8581 tacgaaaggc gagtttgcga aggacttcac gacaatcatt gcaaaagagg tttcaaaatg
     8641 agtgtagaag tcgatgcaac cgaagtgtac aaagcgctta gggaagtaaa agcaaacgtt
     8701 caacgagtgg aaagcccagc acttagaaaa gctggggagt acgctcaaga aaagttacaa
     8761 caaaacacac cttactggga tggaacgaag tcaaacggta aacgtggttc gtatatgcaa
     8821 gaacatgcta aggaccatgt ggttacaagc tcggtaaaaa acggattgat agaagtcggc
     8881 tatgataaag atgtttcttg gcggatgcac tttatcgagt tcggaacaat caaacaacgt
     8941 ccaaaaggtt tcgtacaaaa aacacaaaag caaatcgaaa aacaagtaac acaaatcatt
     9001 gctgacgaag taaaaaggag gctaggactt tgaaaacggc agtatcacaa gtctattcaa
     9061 ttctgaatag caatgaaaaa acaaagaaca ttgattttta caccaatagt gttccggaat
     9121 cggctcaaac agtacctagc cttccagttg gcagaattac agagatattc ggcaactatg
     9181 aagatttcgc aagcaacaat cctttgacca ttcaatttaa cgtacaggta gatgtatggg
     9241 tatcgacctt aaaagaggtt gatgcctttt attttgccct tgatgagatt atgaggggga
     9301 atggttggca atgcgcatac acggaacaaa cagatgatga ggatttagaa ggtgcaaagc
     9361 ggattatcaa acgatatgta gcaaatattt cactaaatta aaaggagaga aaatagatgg
     9421 caacagtagg atttgagagc gtcatttttg gcgtaaaaac aggtgcaggc ggcactctaa
     9481 aagaattagt agcagataag tcgaaaggcg gagcgatcga agctaaaatt actggattag
     9541 gcgcaacttc taacacaaca tacgcttcaa acgtaccatt cttcattgca agtaaagggg
     9601 tttcgtcgcc aaaagttacg cttgacgtgg cagacttaat ggataacggc atttacagcg
     9661 aaatcattgg cgctaaaacc gtggatggtg taaatgtaat tggttcagaa actgaagcgc
     9721 cttacgtgtc ggtagtcatg gttacagcga acaaagaagg aaaacgctta ttcatgggat
     9781 tggcaaaagg aaaattcagt catccagata tcgacatgaa aacagctgaa gacaaaggag
     9841 ttgaattgca aaccgattct atcgaagggg aattcatttc tgatgaacgt ggctatgtat
     9901 acttaacagc cgtagaatca gaagaaatga ccttacaaaa attcaaggac ttggtaaata
     9961 acaaagcggg ggagtagtta accctgcatc tacaccaacg acagatacag ggacaccaaa
    10021 agaaccagaa ccaaaaattg atacacaagg ttagccattt tttggctagc cttatttttt
    10081 tgtaaaaaca aggaggaaaa caaatgattg aattgcaatt gaaacttgac ggaaagaaaa
    10141 aaacattcaa acaacaagat atttccgcac gtgcaatgcg tgagtgtatc aaattttacg
    10201 agaaagcgga aaaagcagac ctaactgatt tagaagcaat tgattcaatg attgcaatta
    10261 cagcagatat tttccaagat ccagcagtta catttgatgc tattttagac ggtttgactg
    10321 cgagcgagtt agtaccggca ttagaaagtg tttttgaaca aatcaatgaa ctgggaaaca
    10381 atgaaaaaaa gcagacggcg agcaaaaaga gataagtttt tctgaagcta ggaaagcaat
    10441 ggatcaaatc tacaaagatt taatcgaatc aggttggacg atgagagatg tggacgaagc
    10501 cgactatcat tatttgttac acctttttgg agaagtggag agtggcgaag aatatgtaga
    10561 tggtgctgat ttcatcaaac aatttttatc ggctgaagac ttggtaaaac ttgaggaagg
    10621 aggtaaataa tggcaggaaa aggacaaccg gcaggaaata tcaagctagg gattagttta
    10681 gatagcacta attttggtaa cacgctggac gaaatcaatg cgaaagtcaa acaagctgag
    10741 tcgaatatgc gtgccaatct aaaggcttat gattcagcag gacgttcata cgaagcactt
    10801 agtcaaaaga cgaaagactt gtctacggtt atggaagggc aaaacgccaa agtaagagaa
    10861 ttaacaaagc gccgtgatga agcgattagc aagtatggcg aggaatcgaa acaagttgct
    10921 aaccttaaca cacagataaa caatgctacc gcaaaatata atgcttacag tcgccagttg
    10981 aatgacacaa aaaaagaatt ggtgtattcc aaaacagccg tcaatgattt atctaacgaa
    11041 atcaaagaaa atgaacgaca aatgaacgcc gaagtaaaag cgttgaaagc cgctggtgat
    11101 gaatctggtg cgtttgaagc aaaacaaaaa gggctagcca aacaaacgga attatccgag
    11161 aaagctatcg aagaacagcg caaagttgtg aaactgatgg ctgatgagtt tggcgattca
    11221 gcaaatgaaa ccgaagatgc aaaaagggca ttagaaaagt tagaacgaca aagccaaata
    11281 tctagcaggc aattagaagc actcaaaagc tccagcgatc aatcaggaaa aaaaatagaa
    11341 gattttggcg acaagtccac aaggtcagct aggaaactgg atgggctaaa agacaaatta
    11401 ggctcgctaa aaagcgcatt ttcgtttggt gcagttgctg gattagcgca taacgctatt
    11461 agcagtgtag taagtggcgt gcaaggcttg gttggcgaag cagtaaacgc atcggattca
    11521 ttgatgaagt tttccaaaac catggagttt gctaactttg ggaagtcaca gatagaaagc
    11581 tcgaaaaaag aaatgaaaga ctacgccgat aagacggttt atggtttaga agaaattctg
    11641 aacacaaccg cacaattggc atctaacggg attcctaact atacagaact aaccaaggcg
    11701 gcaggtaact tgaatgccgt tgcaggcggt tctagtgata cattcaaatc cgttgccatg
    11761 atgctaacgc agacggcagg agctgggaaa ctaacaactg aaaactggaa tcaattagca
    11821 gatgcgatac cgggcgcttc agggctgtta caagacgcta tgttgaaaaa cggagcttat
    11881 acaggaaact tccgtgatgc aatggcgcaa ggtcaaatca cttctgatga gtttaaccaa
    11941 gcgattgtac agttaggtat gaatgacgga gcagttaaag cagctacttc cacagataca
    12001 ttgagtggtt cttgggaaca gatgaaatct actgtaataa atgggttaca aagtatcata
    12061 gacaaagtag gcgttgaaaa tatcactggt ttcatcaaca gagtaacaaa agggattgaa
    12121 aattctattc ctaaaattac tcaatttata ggttggttga gagatattgg aacgtggatc
    12181 gttgaaaata gagagccact aacatggatt gtcggaatca taggcggaat tacattagca
    12241 gtaaaagcat tgaacgtagc aagtatgttg ctggcaatta ctggcggaac attggcagcc
    12301 ccttttgtgg cgattggtgt agcattaggc gcactagcag gtgctttggt gatagcttat
    12361 acaaaatctg aaacatttag aaatatagtc aatgcggctt ttacagctgt gaaaaacgta
    12421 gttatgagcg ttatcaataa tttggtggaa tactacaaaa tgttgtggag cgtgttgcag
    12481 tggctttggg aaaaaataaa agaatgggct tcatggattg gcaataaatt cattgaaatg
    12541 aagaacagcg ttgtgaatac ggttcagaac ctatggaatg gtgtgaaaaa cttcttcagt
    12601 aatggcgttg gagacacttg gaataaggta gttggctggg taaaaaacat tttcaacaaa
    12661 gcaactgaat tgaagaacaa agtttctgat gcaattggta acctgtggaa cggtatcaaa
    12721 gacacattcc gtagaggcat tgatacggta ttcaattggt tttcagaact accgacgaag
    12781 atgaagaatg ccattattgg cggtaaaaat gccattgttg atgcgttcaa aagtattttc
    12841 aacgcagcac ttaaagcgat aggtaaacca gttaacgcaa tcatccatgg agcttcatgg
    12901 gtactagaaa aactgggtgc tgacaaactc gaagaatgga aagtgccaca atacgcaaaa
    12961 ggaacaccaa acggaggtca tccgggcggg cctatgatgg taaatgacgg tagaggtgct
    13021 gaagcggtaa tcacacctaa cggacaagca tttatcccac gagggcgaaa tgtagtgttg
    13081 aatgcaccaa aaggcacaca cgttctaaca gctgaagaaa cagcttatat gactggaaac
    13141 aaagcaccaa gatatagata cgccaaaggt acaggctttt tcggaaatct atggaacaac
    13201 gtcaaaggat ttgctggaga tgttggaaac aagctgaaag atgtagtcgg cgatgtatgg
    13261 gattttgtaa cagacccggg agcgttggct aggaaagtgt taaatggtct tggcgtactg
    13321 gaagggcttg tcaaatatcc tttagatgtt ggtaaaggta ttctaagcaa ggctaccgaa
    13381 gcattgacga acaaaatcac agaactattc agcagtggca gtttagacac ttcaatgggc
    13441 atgcaaggcg tttacaaata cttggcggac gttgcagttg cagtaatgaa gaagtttcca
    13501 ggctttgtgg caactagtgg gtatagacca ggtgacccct attcgcatgg taaacgtaat
    13561 gccattgata ttgcactacc tggtgtcaca ggaggctcgc cacgctacac agaagcagcc
    13621 aattacgcat ttgagaagtt tgcaaacaaa atcggctatg ttatcacaaa tggcaaggtt
    13681 cgtgaccgtt caggacaatc aggtacaggt attcacaatg attggagacc atggcccgat
    13741 ggagatcact atgatcatgt gcatttaaac ggtgtgaaag acccacaaaa cactcaaatt
    13801 tcaggagata gcgtgggagg cagtggggta gaaagatggc gcaatgtagc aattagagcg
    13861 ttgaaaatga ccggtcaata cagtactgca aacttaaatg cattactaaa tcaaatgcgt
    13921 acagagtcaa atggtaatcc taatgcagtt aacaattggg atattaacgc caaaaatgga
    13981 acaccatcaa aagggttgct ccaagtgatt gacccaacat tcagacagta tgcaatgcca
    14041 ggattcaaca gcaatatttt tgacccacta tctaacatct tagcttcaat cagatacgca
    14101 ctatcaagat atggctcact aacaaatgcc tatcgtggag ttggttacgc aaacggtgga
    14161 attgtaaacc aacatcaaat tgcggaaatc gcagaaggaa acaagccaga aattattatt
    14221 cctttagata aggctaaacg atcaagagcg atgcaattat tgtctatcgc aatggacaaa
    14281 ctaggagttt ctcctaaaac ttatggaaat gcgacaactg tttccagtga ttacgaaatc
    14341 ctagaagcca ttgaaaaaca ggcggcaaca acgaatcagc tgttatcgtt attgctggca
    14401 tttttcaaag gaaatagccg aactgataga gatttagctt tagatattca gaagatcttg
    14461 gttaggagga tgtagatgcg aactgtatta ctgagaaata gaataaacga agaaattgat
    14521 ttgtctacgg aggattattt tgctactggg ttgagtaata tggggtttga agtaaaaaaa
    14581 gaacatgtgg gacagtgggg gaatttcaga gaaagcagtg agaacgttga aatatctgaa
    14641 tttcagtcat ctgtaatcat ttctgtgcat gggtttcgag aaaaagaact gtacaattca
    14701 cttgtgcagt tcctatcgga aggtccgttt gaactggaat ttgcttttga cggcgaaaca
    14761 atggtaagaa gatgcagtct aaaatcttta agtaagactg aaatcgatcc gaaaacatcg
    14821 ttactaacag acactttaga actatatttc acatcaaact ggtattccgt aaaacgcgaa
    14881 aaattaattc agcgaccgaa tgtagtgaaa acacgtggta aggtttttcc gtataaaaga
    14941 tcttatatct acactcaaaa cttgtgggag aaaaaaggtg tattcaaatt caataataat
    15001 tctgtatacc taacgaatag caaggaacgg atgtccccgc taaaaattcg tgtgattggg
    15061 aagtgttcaa atccgtattg ggaagtaatc caaaactcac aaatcatcgc aacggatgga
    15121 tacttcatag atatgactga aacacagact ctagaagtat caagcctttt tgaagataca
    15181 acggcgattt taaaagatat tgcaggggta gaatcttctg tctaccaaca acaggattat
    15241 acaaaaacta attttgttca ggccccgact ggagaattca gcattgtgtt tcatgttggg
    15301 ggggcagacg tagagattga gttatacgag gagcgtgatc tgttttgatt ttagcagtca
    15361 cgctttttca tcgtgattta atgctttata acgaacattt attctctaac agttttgaat
    15421 ttggtgcgga cgagatcaac gaagaggcta gtagctttac gatggataag tacgttcctg
    15481 taaaaacagg cgattttcta ctagcaaaat atattcctag tggaaaattt gcatattttg
    15541 gtgtaattac gtcgcaggaa gacgaaaaaa tcagttgtaa aagcttactt agtttagctg
    15601 atagtgagat accgactgca cgtgtgtcag gagataacta cgaagagcat atccggcggt
    15661 tgattgaata ctatttgctg aatgatccaa cgaaacaact aaaagatatt ttagacgtca
    15721 aagcagaaag tgcgacctct cattcgtatc aagctaccga tacgaataaa cacaagttaa
    15781 gtgcgtatat tctcaatggt ttcaaaaaat acaacgtaaa atggtatttc aagggaattc
    15841 aaaacagaaa aatctataca ggcatacgtg ctgtaaatga atcaatttac attaaagaca
    15901 attcttctga atttagcgat tgggatgtgt ttgttcaggc gccgggcgct ggaaacgaga
    15961 acaagctatt aatcgttgat aaagcaatga aagatataga gaaaccgata atactgtcaa
    16021 catggtattt ggacgaagaa aacaatttga cacaagatgg atcgaaagaa aatatcacga
    16081 aaccaactgt caatttggtt aatatctacg atcaaaccgc agaagataaa gcatcctacg
    16141 aagatgtggc aaaatcagag ttgaaaggca atacgtattc gcatgaaatc aaagtgaacg
    16201 ttgtaagaaa tgcaaaaaat ttgaatgtcg aaacgattga aacagggatg tttgccacga
    16261 tttcttataa aggaaagata tacaagtcgg ttttgacagc ttggcgaata tcaagtgata
    16321 aggagtttgt ggaattgact ttcggaaaca taagaagtcg ttttatggat tattttgaag
    16381 ataatggggg ataaaaaatg gttagcaatg tggatggata tcaatttgaa aacgtgaaag
    16441 taagcgcaga aaatgatgct agactttatc acgttttata taatcggaaa aatcaggtta
    16501 ttgatggtta cgatcagtct atgaatttat cttcaagcgg attaacagta aaagttgctg
    16561 caggagcagc gattattcaa ggtcgtatgg tcgttgttcg acaagaagaa agtataacag
    16621 ttccagcaaa ctcaagtggt tatatagcat taacagtgga tttgacacaa gaagttatac
    16681 ctggatctat tcttccagaa tcggaagaat atgaatggac taataatcaa gtcaagctag
    16741 aatttataac aaaagttata aaaggtaatt taaacaatgg tgataaggtc tataatttac
    16801 cactatgctc agttacttct actggatcaa ctgtttcaat ctcaaagata tcggatagtt
    16861 acgagctgac tctctctaaa ggagaaattt tgtggagtgg gactgcgtta atgcatgata
    16921 ctcaaactgt ccaaccttca aaaaaaatgt ggcagacagt ttcaggtttt ttattagtat
    16981 ggctcccata cgaaaacggg caagcaattg gggatagata tgtaactacg cctttttata
    17041 aagagcgtgt aacttttact aatgttttag gagaaattgt ttcaggattt gatgactatc
    17101 acaaaaagtg gtttagtaaa cgaataaact ataattcgaa tactaatata tttacaggtg
    17161 cagcaagcaa tgcaagtgga gataacgcaa atatggtgct taggtatata gtttcttttt
    17221 agttaggagg tatggaagag tggcaaattt agaaattaaa ttatctgcaa ataagagaca
    17281 gccttatcta cgtcaccgtg ttgttggtag agttggtgat ggggggctca caacaatcaa
    17341 tgtacaactt cttgaggaag atgaaattac accttttgta attaatctaa acggtacttt
    17401 gaaatttgtg ggcgaagttt caaacggtaa ctataccgag ggagaaccag aaataatcga
    17461 ttcgactaat gggttaatta gttacacgtt cactaagtca aatttcagca cgagtcatca
    17521 attcaaacaa gcatattttg aatatgtaga tcctaacggc aaaaaagtaa cttttcagaa
    17581 cttcatcata gacgtgttag aacgagtaga tattaattcg gagcaagcga aatactatat
    17641 ttcttcattg gaaaaattac agagtgaaat gcaaaccact ttcaatcagt tcattagtga
    17701 taaacaggtc caatacgatc aaatctactc gaaatataac gaattagtaa gattgataaa
    17761 tgaatcagat aagcaagtaa atgatcgtat tgataagaca aatgatcgta ttgatcaaac
    17821 caatcagcaa atcggcgatc tcggcaagct gaaaaagatg tacagtaaca gcatcgactt
    17881 cggggactat gattatagtg ggaatccgaa ccttagcgct aaacttaatg cttcaagttt
    17941 ttctttaggt actggcgcaa cagtcgcgga tgataatgat gagattgtgt ttaccttaga
    18001 tggcacaaat caactatcga agtacgctag caaagcgcaa gtgccggtag cagaagggaa
    18061 acaatatacg attagttgtg aaattatgtt agaagatggg ttcactggcg acccttcagg
    18121 aatacgtctg caacacgcat atttgccagg cggggttgtt gttttacaga cagagacagt
    18181 acctaaaaat gaattgaata cgtggcagac gcttatcgga acgcgaacgg taaaatacac
    18241 ctctgacgta ccaaatgaat ggtacccttt gtttagagac atccgaacac ttaaaccgac
    18301 cggtaaagtt agactaagaa aaattaagat tgaagaaggc tcaacagcca ccccatatca
    18361 gccaaactta cttgatgacc cttactggct aggtaaagcg cctttgggtg agaatattgc
    18421 taataagtct gttacgtttc caatcaaatc tagcgcctac agcctatacc aagctaacat
    18481 ggaagaagaa tttgttttag gtcaaaccta tacaattacc atgaaggcaa ccaaaccacc
    18541 tatccaaacg ttcattgttt acaacgagga tagtggagat tatagatatg gtaacttaga
    18601 gccagtagaa gggttagttg atacatgggg tctgactttt acaccgcaga aggttggagt
    18661 taactacccc aaacggttaa ctattatcca gtatccccaa tcaacaacag gtgcatgtca
    18721 aattgactgg ctcaagatcg aaaaaggcaa tactcgaacc ccgaatatta gtgagtataa
    18781 atatcgtggt actggtatgc gtgattcaaa caatccaaaa gattatgttt gggatctagc
    18841 accagaatat gtcgaagata atttggccac agatattaaa atttctgaaa ttactggtaa
    18901 agcaaacaat tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat
    18961 taatgaagta gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc
    19021 tatagatgaa ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt
    19081 aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc
    19141 tatatatttt gtggctttaa acggaaaaag cagtggtgga aatgattcaa cgatactaac
    19201 aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg
    19261 ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag
    19321 aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt
    19381 gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg
    19441 tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg attctaaatt
    19501 ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg
    19561 tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac
    19621 agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca
    19681 aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga
    19741 tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca
    19801 gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa
    19861 agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa
    19921 tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta
    19981 gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttt actgacgatc
    20041 aagttcgact attcgttcca ctatgcatta cagacgaaga agcagataaa atcattagca
    20101 aagaagaaag cgcatcttaa gtgatgcgtt tttatttaag gtaaaggagt tgtcacatga
    20161 tcaatttagg ggaatgggga acaatcgcag gatcaattac tgcgattgtt tctttgattt
    20221 tattagtaat aagaccagtc actgcatctt tctcgaagat tactgaaact ctttcaaaag
    20281 taagtcacaa tttagatttg ctgactaaag atttagaatc gagcaaatca gatcgattga
    20341 tgattcatga agaactaaag aaacacgatg aaagattaga tacacatgca gaaaaattgg
    20401 tagaacacac acaacaaatt aaaactttat ttagagaaag gtctcggtaa aaaagaaagg
    20461 agttaagaag aaatgatttt acccgataag tattatcaag tcattaaatg gacagtttta
    20521 acagtattgc cagctttatc tgtattagta gccacattag gcaaagcata tggatggaat
    20581 ggaacagata tggcagttct gactatcaat gcagcagcaa catttttagg tgttatcact
    20641 ggtgtgtcgg cttataattt gaaaaaatag gaggaaacaa atgaaaaaga aaattactat
    20701 tactgcgatg agcctattaa cggctctttt tttattgccg attaatgggt ttgcctatac
    20761 gattaacaat gaatttaatt taggtgtaaa cgaaggtagt tctcaagtag caaataatca
    20821 gtacatttta ctgcatgaaa cggctaatga aacagcaaca ggacgcaatg aagcgcagta
    20881 tatgcaacgt tcatggacta gcgcttatac tgcttatatt gtgggagacg gcggaattgt
    20941 ttatcaagtc ggtcaacctg gttatgtaca gtacggtgct ggttcgtatg ctaatgcaaa
    21001 tagtcctgtg cagattgagt tacaacacac acatgataaa gcaacgtttg agaaaaacta
    21061 caaggcatac gttgaattag ctagagattc agcaatgaaa tatggtattc cacttacact
    21121 agacacaccg tataatcaac cagggatcaa atcgcattta tgggtaacac aaaatatttg
    21181 gggcgatcat acagatcctt acggttatct ttctgaaatg ggcgtaagta aagaaaaatt
    21241 agcctatgat ttggctcatg gatttaccga tgaaaatccg acaacttcag atgataaacc
    21301 agtcattgat ccaactcgag caggtgctgc aaatcctacc ctgacagatg gaacaaatta
    21361 cgcccacatt gatcagttcg gagaaatcga aaacgcaaac ttacatgtgg ctggttggca
    21421 catcgctaac tataaatacg agtatatctt cattatggat tacaatactg ggaaagaatt
    21481 agctcgagta aaagctgatg gaatatatag accagatgta aatcaagctt ataatacttt
    21541 aggaaatgtt ggctatcatg tatcttttaa catgcgcaac ttccctaata agaaagtcta
    21601 tgtaatgatg cgggcaacga atgatccaga agggaacact aaaggcggag cacaagattt
    21661 tcatgataaa cgctggtatt taaatattcc gcaacgataa aaatagcccc t
//
LOCUS       DAYYCQ010000047        21375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000047 DAYYCQ010000000
VERSION     DAYYCQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.047"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..758
                     /locus_tag="ACHAYF_001737"
     CDS             57..758
                     /locus_tag="ACHAYF_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8579176.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1367..2383)
                     /gene="tsaD"
                     /locus_tag="ACHAYF_001738"
     CDS             complement(1367..2383)
                     /gene="tsaD"
                     /locus_tag="ACHAYF_001738"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8579177.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2380..2844)
                     /gene="rimI"
                     /locus_tag="ACHAYF_001739"
     CDS             complement(2380..2844)
                     /gene="rimI"
                     /locus_tag="ACHAYF_001739"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8579178.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2851..3393)
                     /gene="rimI"
                     /locus_tag="ACHAYF_001740"
     CDS             complement(2851..3393)
                     /gene="rimI"
                     /locus_tag="ACHAYF_001740"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8579179.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3377..4099)
                     /gene="tsaB"
                     /locus_tag="ACHAYF_001741"
     CDS             complement(3377..4099)
                     /gene="tsaB"
                     /locus_tag="ACHAYF_001741"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8579180.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4290..5270)
                     /locus_tag="ACHAYF_001742"
     CDS             complement(4290..5270)
                     /locus_tag="ACHAYF_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8579181.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5294..6778)
                     /gene="galT"
                     /locus_tag="ACHAYF_001743"
     CDS             complement(5294..6778)
                     /gene="galT"
                     /locus_tag="ACHAYF_001743"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8579182.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6790..7779)
                     /gene="galE"
                     /locus_tag="ACHAYF_001744"
     CDS             complement(6790..7779)
                     /gene="galE"
                     /locus_tag="ACHAYF_001744"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8579183.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8027..8194
                     /locus_tag="ACHAYF_001745"
     CDS             8027..8194
                     /locus_tag="ACHAYF_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8579184.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8256..10067)
                     /locus_tag="ACHAYF_001746"
     CDS             complement(8256..10067)
                     /locus_tag="ACHAYF_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8579185.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10064..10429)
                     /locus_tag="ACHAYF_001747"
     CDS             complement(10064..10429)
                     /locus_tag="ACHAYF_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8579186.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10592..10987)
                     /locus_tag="ACHAYF_001748"
     CDS             complement(10592..10987)
                     /locus_tag="ACHAYF_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8579187.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11005..11967)
                     /locus_tag="ACHAYF_001749"
     CDS             complement(11005..11967)
                     /locus_tag="ACHAYF_001749"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8579188.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11967..12179)
                     /locus_tag="ACHAYF_001750"
     CDS             complement(11967..12179)
                     /locus_tag="ACHAYF_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8579189.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12200..12898)
                     /locus_tag="ACHAYF_001751"
     CDS             complement(12200..12898)
                     /locus_tag="ACHAYF_001751"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8579190.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12918..13460)
                     /locus_tag="ACHAYF_001752"
     CDS             complement(12918..13460)
                     /locus_tag="ACHAYF_001752"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8579191.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13592..14596)
                     /locus_tag="ACHAYF_001753"
     CDS             complement(13592..14596)
                     /locus_tag="ACHAYF_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8579192.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14593..15582)
                     /locus_tag="ACHAYF_001754"
     CDS             complement(14593..15582)
                     /locus_tag="ACHAYF_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8579193.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15579..16385)
                     /locus_tag="ACHAYF_001755"
     CDS             complement(15579..16385)
                     /locus_tag="ACHAYF_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579194.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16551..17507
                     /locus_tag="ACHAYF_001756"
     CDS             16551..17507
                     /locus_tag="ACHAYF_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8579195.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17584..18102)
                     /locus_tag="ACHAYF_001757"
     CDS             complement(17584..18102)
                     /locus_tag="ACHAYF_001757"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8579196.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18190..18339)
                     /gene="rpmG"
                     /locus_tag="ACHAYF_001758"
     CDS             complement(18190..18339)
                     /gene="rpmG"
                     /locus_tag="ACHAYF_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8579197.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18567..19013
                     /locus_tag="ACHAYF_001759"
     CDS             18567..19013
                     /locus_tag="ACHAYF_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8579198.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19208..21103)
                     /gene="asnB"
                     /locus_tag="ACHAYF_001760"
     CDS             complement(19208..21103)
                     /gene="asnB"
                     /locus_tag="ACHAYF_001760"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8579199.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6426 a         4544 c         3692 g         6713 t
ORIGIN      
        1 aattctttaa aaaaagtgac aataaatata gtgaacaata aggagaggac tttttaatgc
       61 gtgttaataa aatcgatttt cacttttcaa aactcagata tcaacctgat tttgaacatt
      121 tctcggatga agagttccaa cagatcaagg aacatactgt tcttcgttct tataaaaaag
      181 gacagatcct tttcgacgat ggtgatccca gacaacggtt gtatatcttg accaaaggac
      241 tcgttcgttt agaacgttat gatgaaagtg caacttatta ctactatgac tatgtgaata
      301 caaataacct ttttcctatg tcaggaatgt ttggagaaga gacctacact tatacagctc
      361 aagcgatgac tgatattgaa acattctacc ttcctgtttc ctactatgaa aaattagcac
      421 gaaaaaatcc cgaacagttg atctatatga tacaaaaaat ttctaaagta gttgaaatac
      481 atgaattgcg tatccaaatc ggattgacct ctagcgcctt tgaccgggta aaacaaaatt
      541 tattcatttt gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc accattcctt
      601 atccgatcac cttaaaagaa ttagcagtga atagcggcac gacacgtgaa actgctggac
      661 aagtaatcaa acaactacgt gattccgggc tgttagatta tgacaaaaag cgttttactt
      721 tctgtaagac agctactact ccagctatga gagaataacg cgaacaaaaa taagcagtga
      781 ccctgataaa tatcagggtc actgcttatt ttcctcccta aaacttctga aagatccggc
      841 agttttagat cactggacaa gactcaaaga ttttctatgt gtgtttcagt tatgtaatat
      901 tcactgtttg caaaaaatac tgttagatta actagatgaa gcgtcttgtc ctcatccgat
      961 ctagattatt cgatattcct cgacaataaa cgattactca gtgagggcaa tcagaggaac
     1021 ggataatgat aaataagctg taaaaaatag caaaataaca ataatgatta aaagtatgta
     1081 aaaaataggg ctttgacaaa aacgtgtcac agccctattt cgattaaacg gtgtgtacct
     1141 gtcaactctt cggtcacaag tcataatgtt cataaacatg aggagctgtt ttttgaaatt
     1201 gttccttgtg aatcagagtt aaacgacagg cttacgaact cttgattaaa cggcgttcaa
     1261 aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa catgagaagc cgtttttcct
     1321 gggctgttct tattacttga tgcagggcag ctttttcaca acctctttat tcagataaaa
     1381 ccaagcttgg atcagcattc aactgatagc tagcaaaatt tcttttttgc atttccacat
     1441 gtgctgctgc tccaatcatt gcagcattat ctccacaaag tcgaagaggg ggaatcacta
     1501 gttctacctc agggagttta gctgccaaag catcctgcaa tccttcacgc aacccttgat
     1561 tggccgctac acctccagct ataatcagtt gcttgactgg ataattttga caagctcgaa
     1621 gtgttttagt aatcaataca tcgatcacac ttgcttgaaa gctagctgct aaatcatttt
     1681 tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa attgataaat gcacttttta
     1741 atccgctaaa actgaaatca taattatctt catggatcat cgctcgcgga aaatggtaat
     1801 tatcttttcc ctgatgagct agctgatcga tttcttttcc gcttgggtaa gaaagaccta
     1861 atactcttcc aactttgtca taagcttctc ctgctgcgtc atctcttgtc tccccaatga
     1921 tttcatatga tccatcttct tgcatataga ctaattctgt atgtcctccg ctaacaagaa
     1981 gtgccatcaa tggaaactgg aatggtttaa ccagacgggc tgcatagata tgccctgcca
     2041 tatggtttac aggaataagc ggcaattggt gtgcccaagc aaaagcctta gctgcagaaa
     2101 taccaatcag caacgagccg acaagtcctg gtccataagt gacagcgact gccgataaat
     2161 cttctgccga cacaccagct tctaccagtg catcttccaa gcataaggtg atttgttcta
     2221 cgtgatggcg gctagctact tcaggtacta cgccaccaaa acgtttatga ctgtttattt
     2281 gagaagcaac gatgttagat aagatctctg ttccatttct aacgaccgct acactcgttt
     2341 catcacaact tgattcaatc gccagaatca attcttcatt catttcatcg tctccgtttt
     2401 taactttctg ctcataatga tcgcatcttc cgtcggattg cgataatatg atttccgttt
     2461 acctaatata cgaaactttt ctgattcata aagttttctt gctgcttcat tagatagacg
     2521 tacttctaaa aaaacatgca gcgaatcttt tatttgttcc gtaatcagta attgtaacaa
     2581 atgccgtgca tgcccttttc cttgttctga aactgcaaca gcaatatttg tgatctcgac
     2641 ttcatctatt actttcgagt atccaataaa gccacagatc tggttatctt cagctaaaag
     2701 cagataatca gagtgaggct gttggatgtc tgccaaaaac tgcattttcg tccaaggtga
     2761 accatgtgga taagcatttt cgctaatcat ccatacgcat tctgccaagt acgcttcatc
     2821 aaaatctttt tttctgcaaa tcaactaatc tcactcattt tctaaaagtt ttatcatatc
     2881 caacgcatct tcttttgttt cagtgtaata tctttttttg atcgttgttg cttgaaaccc
     2941 caatttccga taaagtcttt gtgcatcttg attactcata cgcacttcta atgacatcgt
     3001 ctcacagcga ttcattatag caaagttctc gatttcgtca ataagcagcg aaccgattcg
     3061 tttacgctga tatgccggtc gaacggcaat attcgtgata tggcaatcta aaccgaacgc
     3121 tcttgaaccg ataaaaccaa tcattcttcc attatcaaag atacctagat atagatgagg
     3181 aagcggtgac ttcaattctg atataaaggc actttttgtc catggcaatt cgccattata
     3241 gacttctcgt tcgacatcca gcagctcttt gatatcttct gtagtcaatt ctcttagtac
     3301 gtaacgacga tcgatttgtt cgactgtttt ttctgtataa atcattttat cttgaaaaaa
     3361 gcttctggca tacgctctaa attttttcaa cataactttc gtctccaggt gtgtgcgtct
     3421 ccaaccattt ttcttctgct tctactcgtt ttaaatagcg aggaagaaaa gcttgtttct
     3481 ctgcaactgg ttcaactaaa gcacctaatt gagccacagt tacaccactt ggaatatccc
     3541 atgctggaac tgtattaatc atcgcactag gcaatatttc ctttatttca gagaagaatt
     3601 tgtgacaatc actcccgacg aaataaatag cttcattcag cttttgtagt tgtgtgaaca
     3661 aatcagaagc agaaatatgt acatcttcaa gtacagtttc taatctattt tctttccagc
     3721 ggtatgcccc agcgtataca ttttttcttc gagcatcaaa catgggcaca ataatattat
     3781 ccacaccgat gcaattagcc gcgatggttt tcagactaga tactcctact agctctttat
     3841 ctaatgtata agcaatcgtt tttgctgtcg ttacacctat acgaagccct gtataagaac
     3901 cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg acttatttga agatctgcaa
     3961 acaattggtc gattgcaggc atcagtgtta cactgtggtt gcgtttgata tttgtttgta
     4021 tttgtccaag aatttgttcg tcttctacca caccgatcgc caaagtttgg tttgcagtat
     4081 ctattcctaa tgtaatcata tatttgatcc tttctgctat gacattctcg ccttccattg
     4141 tagcatactt ttttattctg aggatgaaaa taccaaaggc aaaactagta caataataca
     4201 tgattatctg ctggcgggca aattttgccc cttcctgaaa cacaaaaaag ctgcggtatc
     4261 actccatttt cccacagctt ttccatcttt tatttcgttg attctcgaat aatcaactct
     4321 gtttccacaa taatctttct tgaaaccggt gctttggaat tacttaattg aagaatcgtc
     4381 tccactgcta gctctcccat ccattcggta taaactttta cagtagaaag cgagggcgtt
     4441 acatatttcg ctacactgac atcattgaag cccatcacag aaatatcttc tggtaccact
     4501 aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta atgcatcatt agctgcaaaa
     4561 agcgcatcag gaacatgcgg attcgttttt aaatattgct tcactgcttg atatcctgac
     4621 tcgacagaaa aatctgcttc gataattgga ccttcgtcgt agcctttttc tgccaaaact
     4681 tgttgaaatg ccataattct gggatctttt agccgctgat ggtttttctt cgtatattct
     4741 gttccagcaa gcatagcgat atgttgcgct ttatgggaga caaagtgttc aatgactttt
     4801 tgaacacttg acttaaagtc gacaactaga gagtcgattc cttgagaagt gccgtcagaa
     4861 tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta ataactgagc ttcgtcgaat
     4921 tttcctagtg cgatcgttcc atctgccgga agatcactaa tggaatccca cgtttctttc
     4981 aataaagtaa cgtttgcttc ttctgccttt ttttcgattc ctaggcgaat agataaataa
     5041 taaagatctt caagctcttc cgtatcgtcg taccactgga tcaggcgaag cgtttgctta
     5101 gtatttgctg catttttacg atgtttcgta taatttaagc gttctgctgc ttcaaaaatt
     5161 ttttgctttg tggctaagcc aacggaaaga tcaggatcat agttgagaac tcttgaaaca
     5221 gtagcaggag aaactcctgc caactgcgca atgtctttaa ttgttgccat ttgtctctcc
     5281 tcctttttta cgtttataaa atcgcagtaa atcgttcaaa cgctgcctgt cctttttcat
     5341 ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa aacctgacct acagattttt
     5401 gtacgacatc ggtgaccgta tctttcgtca aatcaggatg ttcttttttt aattgttctg
     5461 cccacggacg atgatattca gccatctggt tttcttctcc taataagaaa tgctctactt
     5521 ctactagttc tggtttcaaa cgaggtggta aaacagcaag ccccattact tcgatcaacc
     5581 caatgttctc ctttttgata tgttggacat ctttatgtgg atgaaaaatc ccatcaggat
     5641 gttcttctga aacattgttg tctcgaagga caaggtccaa ctcaaagtaa ttgtcccttc
     5701 ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc tgctgaaaaa gctctgatag
     5761 atactgtttc atccgaatat gctttccatt tttccaatac tgagttagct gcttcaataa
     5821 gcaagttttt ctctgttgat tgcaaacgga tcacagacat cggccatttc acgactcctg
     5881 cgatgacttg cggataatct tctagtttga taagtttttc tatcggtgct tttgccatag
     5941 gaaattcatg acgtcctgcc tgataatgat catgagaaag aatcgatcct ccaacaattg
     6001 gtaagtcagc attcgaacca acaaagtaat gaggcaagac ttcagtaatc cgaagcagtc
     6061 tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg ttctgataaa atgatcgaat
     6121 gttcattgta ataagcataa ggggaatatt ggaatcccca gctttcaccg tctagattca
     6181 ttcgaataat ccggtgattg gtgcgggctg gatagttcaa gcgacctcta tatccttcgt
     6241 tttccatgca aagcatgcag gtaggatagt ctacttttac cgcctcgcgt tctgctgcga
     6301 tttgttttgg atctttttct ggcttagata aattgattgt gatttccagt tcaccatact
     6361 ccgactctgc cggaaatttg atgttttttg cgatggctct tgttttgatg tagtcatttt
     6421 tcttacttaa attaaagaaa tactcagttg cttcctctgg gcttttcgaa tagtgctgtg
     6481 caaagaatgc attgacgact gaaggtggcg gtgtgagaaa atccattaat tgcgcttcaa
     6541 aaatctcttg ttctgctggc aattctgaaa gaacaccatt ttctcttgcc ttagcgacaa
     6601 gctgatccaa taagtctaca gattcttcct taactggtct tacttcaatg ctttctagag
     6661 catcttctcc taccatccct aaaagacgat tctgtaaata taagcggtcc atttccatcc
     6721 atccgccagc ttcgatcgca agtgtggcaa aatcacaaat cgtttgactg attgacatat
     6781 ctcattcccc tattcttcat aaccatttgg atggctgaca tgccaatccc atgctgtttt
     6841 gataatcgct ttgatatctg tgtattcagg tttccagccc agaatctctc tcgctttttc
     6901 acttgaagcg actaatcggc taggatctcc agctcttctt ggtgctattt ctgctggaat
     6961 ttcttttcct gtcacttctc tagctgcttc tagcatttcc ttgactgagt atcctttgct
     7021 gctgcctaaa ttgaagaagt tgctttcatt tccttctttc aagtattcca aagctaaaat
     7081 atgtgcagca atcaaatctt ctacttgaac ataatctctg atacatgttc catctggtgt
     7141 atcataatcg tctccataaa cagccaaggc ttttctttga ccaagagcta cttgtaaaat
     7201 gattgggaca agatgcgttt ctggtgtatg atcttctccg atagatgcat ctgcttttgc
     7261 tccggctaca ttgaagtagc gcaaagcgac atagcgcatg ccatatgctt ggtcacacca
     7321 cttcatcatt ttttccatca tcaatttact ttcaccatac ggatttttag gattagctgg
     7381 tgtatcttct gtgattggag actctttcgg ctctccataa gttgctgcag tagaagagaa
     7441 tacgatttta ttcacgttga actcgtgcat gacttcaagt aagatttgca taccgtatac
     7501 gttgttgttg aaatacatca atggtttttc gacagattct cctactaaag agcttgcagc
     7561 aaaatggatt actccttcaa tcggttcctt ttcaaatacg gaacgtagaa attctttatc
     7621 ccggatgtct ccttcataaa aatgagcatc tggatgtact gcttgtttgt gacctgtgag
     7681 caaattatct accacagcta cttgataccc cttttgaact agctggtcta cagcgtgcga
     7741 accgatataa ccggctccgc ctagtactaa aattgtcata aaaaacactc ctttgattag
     7801 taaaattgtg tttgttgatt ctatataatt taaaaaacga agtttacttt tatggtgaaa
     7861 acaaacaaag taaaatttcg taaattcata tggtaaattt ataataaaac atttagtaaa
     7921 cattttcaag ggttttttgc aaaggtttac aaacaaacgt aaaactagcc tttttttctc
     7981 ttttgttcca taatagatat aacaaataat aaatgaggtg gattcaatga aaaaatttgt
     8041 atctggaata ttagtaggaa gtctggcaac agccgctgcc gttgctggtt tggttgcttc
     8101 tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa gcaatgatcg aagaaaaccg
     8161 taaaaaagca atgcgtaaac gcgtttctcg ttaataatta tttaaaaata cgcatgaaga
     8221 ctttctaaaa aacagataag tcatcatgcg tatttttatt tgtcagtcaa tataagttgt
     8281 gctattttat ctgcctgtct cagattccat ggatctgtat aaggtctagc tactgctgca
     8341 gttagccaat ccacaccttc aacaggtatt ccatagcaat aaaagtcaga ctggatttcc
     8401 cctgaagcat caatcacgcg attcgtacct cgctcaacat ctatagcttc tgaatgataa
     8461 cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt caaatttttt
     8521 atgacaggat tacttgaacg agaaaagctt gtttgtggca atcgagcttc aatcaaatag
     8581 cgactgctga tttctttatc ttgtagagaa ataacatagc ggttgtgctt ttgctgaata
     8641 tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc taattctttc
     8701 gtccgaatga ttggcggacc gatcgttaaa aagctattta gatgagtaaa ccatccccat
     8761 agtatttctt tgtattcctt agcagaaaaa acgtgccatt caatagcctg ccggattgga
     8821 tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca attgcctttt
     8881 tccgcttctt ttatctgccc ttcaataaat attcggctag cttctgaaaa gggctcattc
     8941 tgataggcaa gaggagtttc taaatacgac caatcccatc tataagggat tagtgctgga
     9001 tgctttccga gacactcctc tgaagagagg gagagaaaat catgctcaaa atttttcttt
     9061 ggaatcggca agtgatattc ttcgatgatt ttcttgtaat aaaacaactc aacgtctttt
     9121 ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca ttgctccagg
     9181 ttttcttttg ttagtaaccg tggccatgcc attgctccgc cttgcttttg atttcttcct
     9241 cgagggtaat aagggagccc ccttcttgat ccactgtata caatcggttc cttgcctgat
     9301 ggatgataga ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga tactgtaaac
     9361 aatccgacat aatcaaaaaa acttaagcct agacctctta aaaagacatt ttctttaggc
     9421 ggtatcttcg aaagatctac atctgcagga ttagaaggaa gctgatacag taaaacattt
     9481 ttctttgcgt agtctaccca cactctttct tcttctgtct catcattttc ccagtgaccg
     9541 cttgctaaaa caagctgatc gatagaatag ctttgctgat ctgtctgtaa aagataacca
     9601 tcttttcttt tgcttacttt ttttactaaa gaataaacaa actgaataga agctggaaat
     9661 tcttcttgca actttgagaa aaaccagcgt tgatagagtc cgtataaaca tcgagtagat
     9721 tgctgatctt tttccagacg attgatttct tctataatta gttgcttatt tttggttacc
     9781 gtctgttcga tataatctgt agcaggtcct ttactccatt gaaacaagtt gggtccctgt
     9841 gttaccaatc ctccactgga caatgtttca tctgtaaaaa gcgtcacttg ttgagaaaca
     9901 gaattcatta ataactccgg tggctgattc aaccgccaaa ctcgacctcc aggaccattg
     9961 ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat tattatggag aatcctttct
    10021 gctacactta atcctctagg tccggcacca atgatcccta ttttcatttg catgcctcca
    10081 tttcgataaa ttttttcaat aaaacagcat ggttctgatg ttcatttttt gcaccataaa
    10141 caaaagtaat gactggatgt tctttcataa gttgactgag tttaaaaata gcttgcttct
    10201 tttcttcttc gaaaatcaat tcttgctggt atttctttgc aaatgaagca aatcgttctg
    10261 gtacatggtc aaaagttttg cgtgcttcat tactaggtgc aatttctttc aaccacagat
    10321 ctaactgcgc tttttctttt gaaattcctc gtggccagaa tctatccacg agtattctat
    10381 agccatcctc tttttcagca tcaaggtaaa cacgttttaa tttgatcata gttgtttcct
    10441 tctttctacc ttttcgctta gcttatatat gtagataata gcacaaaata aaaaaaatga
    10501 acggcacaac tatccattgc gccgttcatg tgatcttggt caaaatctaa tgattgattc
    10561 ggtttttaaa gctgtcatac aatcatctgt tttatacttt tttacttttg atttttccag
    10621 accatccgtc atatccaccc ttaagtaaat aaatctcttg ataacctgct ttacgtaatt
    10681 tatttgctgt acggatgctc aaacttttct tttgatcgta aatatagaca ggctggtctt
    10741 ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt gcacctaaaa
    10801 tatgcccagc atcaaacaca tctttttcgc gaacatcgat tacttgtgct tttctcattc
    10861 cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt actcgtagat
    10921 agacttccca aaggatgatt gcaagcacaa tcaatagcaa aatgatatta attacccata
    10981 aaaccatgta atgcctccgt ttttttaagc aaatttcaag gctaatgagg cagcaccaat
    11041 gacaccagct tcattaccaa gctctgctaa tttgattttt gttgattctc ttacttgcgg
    11101 gaaagtaaat tctttaaaat atttctctac acggctacgt aaaaattctc ctgctgccga
    11161 tactcctcca cctaacacga tacttgacgg atttaatgta ttccccaagt ttccgcaagc
    11221 taaacctaag tagaagcata cgcgatcaac aaccatcaat gcaaacggat cgtcttcttg
    11281 agccatttca aagatatctt tacttgttac ttcttctccg ttgtctagca ttgctttcag
    11341 ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct aggcgtacca cacctgtagc
    11401 gctagataca gtttctaaac atccgcgttt accgcaagta cattcgaacc cttcaggatc
    11461 cacagtgata tgtccgattt ctcctccagc tccgctcacg ccgtgaatca aatttccgcc
    11521 agcaacgata cctccgccaa cacctgttcc tagagtaacg aatacaacgt ctggatcgtt
    11581 ttctccagca cctttccatc gttcacctaa agcagcaaca ttcgcgtcat tatcgatagc
    11641 gaatttgatt cctgttcctg cctcgatcag ttttttcaca aattgaactt ctgtccagtt
    11701 taagttatac gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg gggtacccat
    11761 gccgattccg ataaagtctt cggctttcat attatataaa ttcaaacgat gattgattga
    11821 ttcaatgatt tcaggaacga tatgcttacc atcatccaaa atattggtat cgatgctcca
    11881 tttttgctga atctctcctt ctggtgttaa aattgcaaat tttgctgttg ttccacctaa
    11941 atcaatccca ataatcttct tgtccattac aaatttcctt tctttcggct ttcttcaatt
    12001 cggtgctctc tttttaatat gcgccacgct gtcatgtatg tatcatgatc aatcaaacgg
    12061 ctatcataca gattacgcaa ttcgatagtc attaattcaa tgtcatagat gcgtgcaccc
    12121 acatagataa agatgccaaa ttttttgaac agctgctgta catcatataa gctctccatc
    12181 tttaagcact tccttcatat tatacaagta aaccatattt ttttaaaccc agtaaaatac
    12241 atattacaaa aagaaataca aaaactagtg tagaaagaat cctttggtgt atcgaatagc
    12301 ttgtctgttg tttcggtaga cccaaaacat tccctaacac taatccgcca attagcccac
    12361 cgacatgtcc ccaaacatct acagaggtat ccagaagacc aaaaacaaat gtcaacacaa
    12421 taaataaaag aaaatgtctg actagctgct ggatcgctgg gttatgttta aaatgaaacc
    12481 ctaagataaa caacgcaccg aacaaaccga atatcgaagt acttgcacca ccagacagaa
    12541 cattcgcttc gttaaaagca aagcttgctg tatttcccat tatcccgctg aacatataaa
    12601 tcaagaaaaa acgccaatgg ccatagattg cctcgatttg ctgccccata aaatacagta
    12661 tcagtgagtt cattacgaaa tgcatcagtc caaaatggat gaagattggt gtcactaaac
    12721 gccaccattc attgaagtga acaagataag gaccatacat tccggcataa taagggatct
    12781 caagtcgcgg aaataattcc atcatgagga agaccgctgt ctgtatccct aaaaaagcat
    12841 aggtccataa cggtcgtctt aaccatgcac gcaattttat ttgctgttga taattcatga
    12901 aaacctccca tttcatttta gtcaataaac aattgttgga tcttctgatc aaagctctcc
    12961 ggttgccatt gttcattcaa ttgttcacga aaaacaaggc tacatgtttt tttatgaaaa
    13021 ttatttaaat accggtcgta ataaccaccg ccaaaaccga ttcgataacc ggctctattg
    13081 tagataatac ctggtacaat gaggagatcg atcatctccg gcaaatagag cttctctgat
    13141 tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg actgttcatc catttcataa
    13201 aacgccatct gacgtttcgg caaagtttta ggtacaacta ttttcttttg ttctttttct
    13261 gctcgttgga taaccggctg tgtatccagt tcaaattcat tcgataaagt cgtccctatc
    13321 acttttgctg acttccagta ctcgctggcg aatagctgtt tatagattcg ctgttctttt
    13381 tcctttttct tgccactttt ggccagattt ttcagctttt cgattccttt ccggcgtaat
    13441 cttactttca attggtccaa aaactcaccc ttccttctct cataaaatgg tataggaaaa
    13501 aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa tagaataaaa agaagaccta
    13561 gaaatgattg gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt ttttgtctga
    13621 taaaaagtgc atacagaaaa tatggcgcac caaaaagggc aatcaaaatt cctgtaggta
    13681 cttccccact tggtagaatc actctagcaa ccatatctgc actaagcaga aaaatggaac
    13741 cgaacaaacc gctcagaaca aaggtccagt tattttcttt acgtacgact agcttcgcag
    13801 catgaggagc tagtagtcct acaaaagata aactacctgt caccgctaca ctaccgcttg
    13861 ccaaagcaac agctaacaac aaaaggattt tttgactttt agccacgtta aggcctaatc
    13921 cttgcgcgcg ttcttgacca aatgatagca atacaatcct ctgtgcataa agtcctccta
    13981 gcggaattaa gataaccgcc catggaagca atgcccaaac ataaggccag ctcgcacccc
    14041 agattgatcc cgctaaccat gcattcacaa atccataatt ttccttagat aattggatcg
    14101 tgcatagcaa tgttaatgca gaaaggccgg cattaacggc aataccggac aataagattc
    14161 gattcatacc caaaaagcca tttcgatcat aggccgcaag atagacacag caagcagcta
    14221 aaacaccacc tagaaaggct acaactagca atagaaacgg cagcgaatag tgcgaaaaga
    14281 aacctaaata aagcatcact aaaaatcctg ctccggcatt aatgcccaat atactagaat
    14341 ctgccaattc atttcttgtt actccttgca agatataacc agaaacgcca agacaagctc
    14401 ctgctaataa actgatcagt aatcgaggaa ctcgaaattc tctaacaatc agcgccaaag
    14461 aagatgattc tttccctatg aacgtggcaa aaagctgatc caggtaaata aaaggcgtac
    14521 caatcattaa actacctata gacaggatga caagtacaaa aagcaagaaa tagaacgtcc
    14581 aatatatttt tttcaccgtt gttccctcct tacttgtatc agtaaaaacg gaacgccaat
    14641 caatgcaatg actaatccaa aaggtgtttc aaaaggggga ttgatcatcc tcgctgtaaa
    14701 atccgccaac ataaccaatc caccgccaac taaaaaagag acaggtagaa cataacgata
    14761 gtccgttcca accattccgc gaacaactgg cggaaccaac agcccaataa atgaaacagg
    14821 tccgactaac gaaacggcaa tggctgctaa cactgcaact agacagaaaa caatcaaccg
    14881 cgttcgttta ggatgacttc caagagctaa tgcatgtgtt tcactcaaat gaatcaaaga
    14941 tattgctttt cctaatgaaa aagaaaccaa aacagcaagt atatataaag gaaacaatac
    15001 tgctaattgt ttccatgtga tattggcagc tccgcctaca aaccaaaaag caagatcctg
    15061 ctgcagatta aagaattggg taagtgcttg gctgatagca gtaaaaaaag aactaatagc
    15121 tacgccagct aaaatcagct gtaaaggatt catgcccatt ttcgaaaact gcaaaaaacc
    15181 aaatacgagc aaagttacag caaaagaacc tgccaatgac attagaaaca cagaaaaatc
    15241 agaagcttta ggccaaaaag caaaactaag ggccattgcc aacgaagcac ccgcgttgat
    15301 tcctagtaag cctgaatctg ctaaaggatt tcttgtcact ccttgcatca atgcaccgct
    15361 caatgcaaaa caactcccta caagaaatgc tcccgccaca cgaggcaaac gaatatttcg
    15421 tatgatttgg tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc aaaaatctgc
    15481 tttttccaca gtcgctgcgc catttgaaag agcaacaaaa aaaataatca aaagaacaaa
    15541 acagaccaaa agcaggagtc tatttttctt catagtcctc ataattgatc ctcctgctct
    15601 tttggcaaca aatcataggt tagaaaaact ggtttatttg ttcttggaag cgtgacaatc
    15661 gttgcatcaa tctcaaatat ttttttcata cactcttttg tgatcacttc ttcaggtgta
    15721 ccttctgtga tcaacttacc gtttttcatt gctaataaat aatctgaaaa acgtgaagca
    15781 tgattaatgt catgaatcga catcaagatc gttgtttgtt tcatttgatt gatttcagtc
    15841 aataactgta ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg ttcatccaag
    15901 atcaagatat ctgtttcctg agcaagcgcc atagcgatcc agacacgctg ggtttgtcca
    15961 ccagacagcg ttgcgatatc ccgcatacga aatccagcta gacctgtagc atccaacgcc
    16021 cactcaattg cttcatgatc ttgcgtgtcc atgccactga acagtttttg ataagggtat
    16081 cgtccgtaag atactacttc ttctactgtc atacctgtag ggtgttcact agtttgtgct
    16141 aataatgcaa ttttttttgc aagttctttt gaactgtatg tagagatatc agagttattg
    16201 acaatgatct ttcctgattt cacaggtagt atacgggtaa aacttttcaa taaagtagat
    16261 tttccacttc catttggccc aatcaaacta gtgattttcc cttcaggaat cgtaacagac
    16321 atgttttcaa tgattttttt gttttcgtag ccggctgcaa tgtcagctgc tcttaatctc
    16381 gtcatttcac ccatcctttc gctgaaaatc attctcaatt atgacgctgt ttcaacattt
    16441 cgtcaagcgc tactttcata tctatagcca aatgaagcgg ttgacagcgt tcgaaatatc
    16501 aggaataata accaatgaga aagattctca attatgttag gaggaaagaa atgaaattaa
    16561 aaacaaaagt attcacatta tctagcatat tgctgctggc tggagtactg agcgcttgct
    16621 ccccctcttc agataaagaa acagctacaa ctgccaattc gacgacttca tccaatagta
    16681 ctcatacagt cacagataca cttggacata aagtagagat tccaaacgcg cccaaacgga
    16741 tcatcggcag ttacttagaa gactatctga ttgcactagg ggaaaaacct atcgcccagt
    16801 ggactgttgg cagtggttcc attcaagatt atctgcaaga tgatttaaag gatgtcccaa
    16861 cgatttctta tgaccttcct tatgagaaag tgttaagctt tgagcctgat ttgctgttga
    16921 tttcttcttc cgctactgtc gaaggcggga aatacgaaca atatagcaaa atcgcgccaa
    16981 cttatgttgt aaaaaacgga acagatgtca catgggaaga acaactaaaa gatgtcggca
    17041 aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa aaatccatca
    17101 agaaaactcg agaagaacta gcagataaaa tcgagaacaa gacagctgca gtgttgtggg
    17161 tgaccaacaa ctcagccttt atggtcagca aaaatcggtc aagtggaaga atcgtatacg
    17221 acgatctgga atttggtgtt cctgatctag tggaagaggt ctccaaaaaa gccacttctg
    17281 actggtctgc tgtttcctct gaaaagctag ctgaattaga tgctgactac atcatccttg
    17341 tgaacagtga caaagatgct gccatgttca atgaaccaag ctggaagaat ctaaaagcag
    17401 tgacagacaa tcatgtactg caattcggac ctgaatctag ttggctatat aacggaccta
    17461 ttgccagtca ggaaatggtc aatgatatca aagaaaaact aaattgattt taactaaaaa
    17521 aagcatcttc acgattagtc gtgagatgct ttttttctta ttaacaaact agacgcatag
    17581 attttattct tcttttgaag caaccctatt ttttactctt tcccattttt gaaaacgatg
    17641 ggcatataga ttttcttgat ccgttatccc ctcatactcc tctataaggg cgaagtctga
    17701 aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca atgttcgcca
    17761 gatccgacca gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg aaacatagat
    17821 atcttctgag atcgtttgac tatacgacaa aagttcatcg atcgaatgca ttacttccgc
    17881 attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc gataaggaag
    17941 tgaaagatcc cccattcctt cataggtctt tcttcccatc actaaaattc gatccattgt
    18001 atgttcgcga aagaaacgca tatcatttgg caatcgccat ggtaacaaac catttttgcc
    18061 gatcagacca tttttatctt gtgcccacat ggaaataaac atattatccg ctccttatga
    18121 ttttagatgt tttgagataa aaaaagtctc aattctttga ccagcaagga attgagaact
    18181 ttaaaaacat tattttgttt cacggtgcaa agtaactttg cgttcgcgtg ggcaatattt
    18241 ttgtttttcc aaacgatcag gattgttacg tttgtttttg cttgttagat agttacgttc
    18301 tttacaagaa gtacactcta aagtgatgtt tacgcgcatg tttttccctc ccatttttac
    18361 aagaatcctc taagatgact ctcagacttg attatcttac catgattttt tctagattgc
    18421 tagtgttttt cacgattttg ttaagtaaaa ctacttgacg ataaatacaa cggttttact
    18481 tctcaaacaa aatctttttc tttttgtata aataccctta cacttgttta tatagattat
    18541 gttttttaat aaagggggat actaatatgc catggaacat gaaggacttt cctgcatcaa
    18601 tgaaaaactt agataaattg acaagaaaaa aagcaatcga catcgcaaat gcattattag
    18661 atgaagggta ccctgataac cgtgcgatcc caattgccat tgatcaagca aaaaaatggt
    18721 atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat ccaagcaaga
    18781 cggatgaaca cgatacgaat cctcgtgctg gaaaactgct tgattcagat gtgatcgtag
    18841 aatatgagga agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca agcaaccatt
    18901 ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac aaagaatcaa
    18961 ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga aatcgattat tgaacatatg
    19021 taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca atggtgttcc
    19081 tctgccaact ccgaaccata agtcataata tctataaaac gggaagagct gttttttgaa
    19141 attgtttctt atcactcagt gttaaacggt aggagaaaac ctctttttta ttgatatttg
    19201 attttcatca aacagtcaca tagtcgattg cttctgcttg cggaatcgtt tcatctacaa
    19261 agtagacttt ataccatgtc aagaaactga agattgtcca gatttttctt tgctcttctt
    19321 tacgtccttc gtgatggtca tctagcaact tcatgatttt ttcttgatca aaaaattctt
    19381 ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta gccgtgttct tctttcaacc
    19441 aagctttgat tggcacaggg aatcctaatt tcacacgatt cgaccattct tccggaagat
    19501 gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat aaaaacttac
    19561 ttggtatttg ttgagccaat ttcatgactt cgatatccaa taaaggtact cggacttcca
    19621 aagaacttgc catagaaagt ttatctgcct ttaacaagat atcttttggc atccattgat
    19681 gaagatcgac atattgcatc ttattgactt catctttgat tccttctgtt ttctcgtaat
    19741 gaacagtcat gatttcatcc acagatggtg ctgtttgata ttttggtgtt aacacttctg
    19801 atgcttctga ttcttcgaac actcgagcat gaccgataaa atattctttt gcaggagcaa
    19861 gtgattcata taaatggatc cgtccatgga agtttttcat ttttccgatt ttacgtccta
    19921 aattatagcg tgttcctttt ggtaattttt tcaatccttg agcaatcaca cggatgaatt
    19981 ttgaattcgt atggaatcca tacgtttggt atcccgcaaa cagttcatcc gccccttctc
    20041 cagattggac tacccgcaca ctttgtgaag caagatttgc taaaaagtac aaaggtacgc
    20101 aagaaggatt tgaatcaggt tcatctaagt gatactggat caaagggaat gctttgaacg
    20161 acatgtctcc gtcgatcact gctgctgtat tatctaagtc caatagttcg gttagttttc
    20221 tggcttcaat cgcttcatta taggttttat catcaaaccc gattgagaaa gaatgatctg
    20281 gtttcaatac agaggttaca taactggaat caaccccgct ggataagaaa gaaccaactt
    20341 ctacatcact gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg attttttcga
    20401 tccattcttg tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa tattgctgaa
    20461 tgtctatttt tccatcttta tacgtataat aatggccttc aggcaaacga tagacatctt
    20521 tgaagaatgt ttcgccgttt aatggagaat attggaaagt catataaggt ttcaacgctt
    20581 ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa acttttgatt tctgaaccaa
    20641 acataaatgt tccgttcatt tctgcataat aatatggttt gatcccaaag tggtctcttg
    20701 caccgaataa ctcattcttt tcattatccc aaatagcaaa agcaaacatt ccgcggatct
    20761 tttgcaataa ttctgttccc cattcttcgt aaccatgcaa aaggacttct gtatctgcat
    20821 gtgtttgaaa aacgtggcca gctgcaatca attcttctct caaaggctgg taattataga
    20881 tttcgccatt aaaaataatg atctttgtcc gatcttcatt gtaaataggc tgagtaccgc
    20941 cctctaagtc aataatactt aatcttctga atcctaaagc aacatgttta tcgatatatt
    21001 ctcctgaact attaggtcca cggtgaacga ttcgatccat cattgtatta ataattgttt
    21061 ttttgttgtc cttgtcgtta acaaaaccta caataccgca catttaactt tcacatccta
    21121 atataaaatt actgtttatt tcattcatat gcatcttatc atagaataaa tagcggacga
    21181 atattttctc tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt ctgtttttac
    21241 agaaaataaa gtcccaatat ctaatttacc atgttttctt ttttgaggct accttattct
    21301 cttcgtgtta aaaaaatatt agtggatgct atttccaaaa aaaccagcaa aaaaagacac
    21361 gacctaaaga tcgtg
//
LOCUS       DAYYCQ010000048        21008 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000048 DAYYCQ010000000
VERSION     DAYYCQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.048"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHAYF_001761"
     CDS             591..1280
                     /locus_tag="ACHAYF_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8579200.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHAYF_001762"
     CDS             1267..2475
                     /locus_tag="ACHAYF_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8579201.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHAYF_001763"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHAYF_001763"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8579202.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHAYF_001764"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHAYF_001764"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8579203.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHAYF_001765"
     CDS             complement(6578..7273)
                     /locus_tag="ACHAYF_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8579204.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHAYF_001766"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHAYF_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8579205.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVQLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHAYF_001767"
     CDS             complement(8472..8663)
                     /locus_tag="ACHAYF_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8579206.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHAYF_001768"
     CDS             complement(8684..9574)
                     /locus_tag="ACHAYF_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8579207.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHAYF_001769"
     CDS             complement(9561..10328)
                     /locus_tag="ACHAYF_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8579208.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHAYF_001770"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHAYF_001770"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8579209.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHAYF_001771"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHAYF_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8579210.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHAYF_001772"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHAYF_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8579211.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHAYF_001773"
     CDS             14899..15282
                     /locus_tag="ACHAYF_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579212.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHAYF_001774"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHAYF_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8579213.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHAYF_001775"
     CDS             complement(16226..17044)
                     /locus_tag="ACHAYF_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8579214.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHAYF_001776"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHAYF_001776"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8579215.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHAYF_001777"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHAYF_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8579216.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHAYF_001778"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHAYF_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8579217.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHAYF_001779"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHAYF_001779"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8579218.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         6009 a         4266 c         3846 g         6887 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaattgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgc
//
LOCUS       DAYYCQ010000049        20400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000049 DAYYCQ010000000
VERSION     DAYYCQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.049"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACHAYF_001780"
     CDS             complement(387..1853)
                     /locus_tag="ACHAYF_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8579219.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACHAYF_001781"
     CDS             complement(2144..4228)
                     /locus_tag="ACHAYF_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8579220.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4370..4492)
                     /locus_tag="ACHAYF_001782"
     CDS             complement(4370..4492)
                     /locus_tag="ACHAYF_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579221.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(4753..5112)
                     /locus_tag="ACHAYF_001783"
     CDS             complement(4753..5112)
                     /locus_tag="ACHAYF_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8579222.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5142..5483)
                     /locus_tag="ACHAYF_001784"
     CDS             complement(5142..5483)
                     /locus_tag="ACHAYF_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579223.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5480..6154)
                     /locus_tag="ACHAYF_001785"
     CDS             complement(5480..6154)
                     /locus_tag="ACHAYF_001785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8579224.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="ACHAYF_001786"
     CDS             complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="ACHAYF_001786"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8579225.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8668..9858)
                     /locus_tag="ACHAYF_001787"
     CDS             complement(8668..9858)
                     /locus_tag="ACHAYF_001787"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8579226.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9879..10406)
                     /locus_tag="ACHAYF_001788"
     CDS             complement(9879..10406)
                     /locus_tag="ACHAYF_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8579227.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10453..13242)
                     /locus_tag="ACHAYF_001789"
     CDS             complement(10453..13242)
                     /locus_tag="ACHAYF_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8579228.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13389..13589)
                     /locus_tag="ACHAYF_001790"
     CDS             complement(13389..13589)
                     /locus_tag="ACHAYF_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8579229.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14042..14362)
                     /locus_tag="ACHAYF_001791"
     CDS             complement(14042..14362)
                     /locus_tag="ACHAYF_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579230.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14865..15983)
                     /locus_tag="ACHAYF_001792"
     CDS             complement(14865..15983)
                     /locus_tag="ACHAYF_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579231.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16216..16338
                     /locus_tag="ACHAYF_001793"
     CDS             16216..16338
                     /locus_tag="ACHAYF_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579232.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            16454..17761
                     /locus_tag="ACHAYF_001794"
     CDS             16454..17761
                     /locus_tag="ACHAYF_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8579233.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17882..19081
                     /locus_tag="ACHAYF_001795"
     CDS             17882..19081
                     /locus_tag="ACHAYF_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8579234.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19108..20376)
                     /locus_tag="ACHAYF_001796"
     CDS             complement(19108..20376)
                     /locus_tag="ACHAYF_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8579235.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5906 a         4344 c         3559 g         6591 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacatgact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaatttct tccatacaga tgttttggct tattctttct
     4381 ttttctcttc gatcattgcc cctgctctta tccaacccgc acttgccttc ttgatcttga
     4441 caggatagca actaagtttg aagcctgttg ctggcaattt gtctaaattc attaattttt
     4501 cgataggaca atatcttttt tcaatccctg caaaatgtcc ttcccagata ttcgaagctc
     4561 cactacgctt tcaaaaatat agaaagagtt ctttaatgaa aattatttca actttccata
     4621 aacagtaatc ccatataaat aaaagacgta aaagtcacat gggagaactt atacaggaaa
     4681 acacatagag tcaggtgaaa tacatgtatc ccacctgact ctatgcgttt tctaaccttt
     4741 attattctct ttttatacaa tttctgcacc gaatggcatc aacgccaatc gagcaagttt
     4801 gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga tgccgctgac
     4861 gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat taacgatcaa
     4921 tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag aaagggaggc
     4981 gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc aaagtatccc
     5041 cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga tgacatttcc
     5101 taaacaactc attgtatttc tccttctatt actgtttttg attaaaactg ggcaaaaatt
     5161 gattttattc ttcttttttt agcttccagc atcaagtatt ctgcaaattt tttttggatt
     5221 tcttccgtat tttcttttgt tactaatgtg tgttggatta gttttcctga tggtcgctga
     5281 tccacccgtt cttctgactg ttgcacgtcc gcttgcgcaa atccgataat ccgtttttct
     5341 tttgttacag caacgagcaa tagtttattc ttatcattta ggtcagctgc cgtttttgct
     5401 tttacgaatg attctggatg atcagtaatc agctgattga tttgaggtaa atcttctttt
     5461 actgcccacc gtatttccat catttctcac ttttctttcg ctcattttta tctccactaa
     5521 aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc aatgatctca tgtccgccaa
     5581 tcgttgggtg gaccccatct tctcttgtat atttcgaaaa accataagca atccctaaag
     5641 cattgaatat gccatctaaa ggaaccagca atgcgccata ttccttagca agtttacgaa
     5701 caatttggat tttagggtct aagtctttac gccagccatg acggtcgtcg gttataggaa
     5761 gtacaaacgg ctccatcaag atgatgttgc agatttccgc tgtttgaagt tcatttagta
     5821 attttcgata aacggattca aattgctgcg cagatttctc agttccgaat atagcctgat
     5881 cgctagtatt atgccaggta tcattgattc cgatcaaaat cgatacatag tcaggttgta
     5941 aagctaaaca atcttcttgc cagcgtactt gcagatctgc aattttgtct cctcctatcc
     6001 cacggttcat gcattcaaat ttcatcgttg gatattggga gattagtttt ccggcaatca
     6061 aaagtggata acctttacct agatcagttg cttctgctcg gtctcttcct gcgtctgtaa
     6121 tgctgtcacc gataaataat atacgttttt tcatttgtca aaaactcctt tttgaataaa
     6181 acgatgattt tcttccttat attatcccat aagccctatc aagacgaaag agaaaatata
     6241 tgtataaaaa atgacaccag cctagtccct tcaatcaatt gaatgaacgc tcatcaagtg
     6301 tctagttttt tatacttttt ttcttaacgt gccggattcc ccttgattcc ttcgttgttt
     6361 ggtccagaga aatcgatcac acctaagctt ccgtcaatca gaccactgat gacgccatca
     6421 gaacatatat ttataggctc atctaagact tccagtaaat gttcttttac taatatctct
     6481 tctagattgc ttgggataga ttggtcataa ctttatctgg tccgatgatt tcaaggatga
     6541 attaaactta catatggatc attgcatgct gaaaatacgg acctactcca tcttcgaatg
     6601 ttactattag tttgttttca ggtactagat gctgctttat ttcaacagca gaagcatcat
     6661 caaatcagat ttccatttga ataaccttct ttgtatatgt ttgattgatt gcgatttcgt
     6721 tatagaacct tgtgaatcaa atagcaattt cttttgcttt cttctcttgt ctgctggaaa
     6781 cttcctttat cccgtatttt aaaaaaagaa ctgcgagagt tcgcagttct tttttttaaa
     6841 taaacgttgc tctccggcca actcctcggt cacaagtcac aatagtcatc aaacatgaaa
     6901 aactgttttt tgaaattact ccttgtgact cggagctgaa cggcaggcgc acaacctctt
     6961 gatataaacg gtgttcaatc agctgaccct cgatattagc taaaaaaacg gtaaaatatg
     7021 agaagctatt tttccgtttt gtttctctaa tactcaggtc aaggcactga tttcacaacc
     7081 tcttccttaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     7141 tgtggaaagc catttccaca aaatttttct taatactcga aactaaacag ctttttcaca
     7201 acctctccac taaacggtat tcaaaagctt acatctgcgg taataagtcc cgccaattca
     7261 aaaacatgaa cagctgtttt tgcttggctg ttctaattac ttgatgcaga acagcttttt
     7321 catcacctct tatggataaa ttcggttcaa caaacgtggg aatggaatag cttcacgtac
     7381 atggtcgata ccagcaatcc aagtgactgt tcgttcaagg cctagaccaa aaccagagtg
     7441 cggaacagaa ccgtatttac gtagatcaag ataccatgaa tattcttttt catccaaacc
     7501 gtgattacgg atttgttcta gcaagaaatc atagtctgtt gcccgttcgc ttccgccaat
     7561 aatttcaccg taaccttctg gtgcgatcat atctgcacaa attaccacgt cttctcttgt
     7621 tggatgtggt ttcatgtaga atggcttgat ggcttttgga tagtttagga taaagacagg
     7681 ctgttcgaat gaattcgcaa tgaacgtttc atgaggtgaa ccaaagtctt cgccccattc
     7741 gatgtcatca aatccatttt tcttcagtaa ttcgattgca tcatcgtaag taatacgagg
     7801 gaatggcaat tttgtataag tttttaagac ttctttgtct cgtcctagta catctaatgc
     7861 atattcgcaa tgatctaaaa cattttgtac taagaatgct acatattgtt cctgtacttc
     7921 taaactttct tcttgatgag tgaaagccat ttctggttcg atcatccaga attcgatcaa
     7981 atgacgtcgt gtttttgatt tttcagcacg gaaagtcgga ccgaatgaaa agacttttcc
     8041 gaaagccata gcagcagctt ccatgtataa ttggccactt tgagataaat aagctttttg
     8101 atcaaagtaa tttgtttcga atagatccgt agtgccttct ggtgcagaac cagtcaagat
     8161 cggcggatcg attttgacaa aatgatgttg attaaagaat tcatacgttg cacgaatgat
     8221 ttcatttctg atctgcataa tcgcatgttg tcttgaagaa cgcagccata aatgacgatg
     8281 atccatcaag aaatctgttc cgtgttcttt tggtgtgatc ggataatcgt ggctctcgct
     8341 taccacttcc acatttgtaa ttccgatttc ataaccaaat tttgaacgac tgtcttcacg
     8401 gatttctcca gttactaaca cggatgtttc ttggtgtaga tttttagctg tttggaaaac
     8461 ttcttccgac acttcgcttt ttacaactac accttggaaa aaggctgttc cgtctcttaa
     8521 ttgtaaaaat gcgatttttc cgcttgatcg cttatttgcg acccaagcgc cgattgttac
     8581 tgtttctccc acatggtttt tcgaatcgat gatttgaatt tgttccacgt atgactctcc
     8641 ttttatattc cttgaatttc ttcaggatta attttgactt ttactctcaa taaattgctt
     8701 gattctgcgt accgcttctt ctaacgtagc gcgatcagtg gcataactca tgcggacatt
     8761 ttctggtgcc ccaaagcctt cgccagtcac aagcgctacc cctgtttctt ctaaaagatc
     8821 ttctacccat tgtgtgacat tttcatactt acacatgcgt agcgtttctt ttacattagg
     8881 aaaaagatag aaagctcctt gcggcttttc aagagaaaca cccggcagtt ctgccacaag
     8941 cggatataac gtattcaggc gttcttcaaa ggcgttccgc atttcttcta ctgtatcttg
     9001 ctcacctttc aatgcttcaa cagctgcata ttgactgact gctgtcggat tgctagttga
     9061 ttgggaagca acagcgatca ttccattgat gatcacagga tttcccaccg cataaccaat
     9121 gcgccagcct gtcatcgcat atgttttaga cacgccgttg atgatgatgg tctgattttt
     9181 gattgcttct gaaatcgttg caattggagt aaactcattg ccattataaa ctaaacggcc
     9241 ataaatatca tcagcaacta tcaaaatatc attttttact gcccattcac cgattgcctg
     9301 cagttcattc ttgctataaa tcattccagt cggattcgat ggagaattga tgattacggc
     9361 ttttgttttt tctgaacgtg cttgttctag ctgttcaaca gtaactttcc agctattgga
     9421 ttcttcccct ttcacaaaga caggaagtcc ttctgccagt tttacttgct caccgtaact
     9481 gacccagtaa ggcacaggaa tgatgacttc atcttgcggg tcaaggatcg tttggaaaag
     9541 ggtatacaaa gcaaatttcg ctccgtctgt tacgactgtt tgtgaaggtt catagacaag
     9601 tccatagttt ttcttcagat aatcaacgat tgcctgtcga agctctggga ttcctgctgt
     9661 tggtgtataa tagctagctt tcccattgcg gattgcttcg attgctgctt cttggatgtt
     9721 ttctggtgtc gcaaaatccg gttctcccac agtcaaactc agcacatctc tgccttttgc
     9781 ttttaatgcc ttggcctttg ccgcagctgc caaagtgaca gacggttcta atcgttttgt
     9841 tctttttgat aactccatcc cattcattcc tttacctttt aaattccctt gattgtcttg
     9901 acttcatctc cagattcaaa gccaatcagg taataattca gtttcccatt tgtttctttt
     9961 cctactactt cccacacagg ctgatcatca tacatcccta aagcagcttt ttcgatttgg
    10021 atttcaggat gggccgcagc aattgtctgt tttgcttcgt tttcagtcag tccctttgtt
    10081 tgatccaata cttttacttt ttcaccagat ttaggaatga taacggcgat ttcttttcct
    10141 ttatcatttg tacctgtcag tgaaaagtat gtcttctttc gagtaaacca ataaaaatga
    10201 tcggtttcct caagatttgc atatttcttt gcgatttcta ctgcttcttt cttcgcctgc
    10261 accgccggac gactggaacg caagtaaaaa atcgagctga tcacgatgac cgctaataaa
    10321 aatacgatca agccaagtaa cacgcgatta atcgtttttt cctttgtctg ttttttcttc
    10381 aagggtcgtc gctcctttat cttcattcct tttcattata gcagatttta attatgaaaa
    10441 aagacagctc ctctaacttc cttcttcatt tttctttaag aattcatgga tatcctgata
    10501 gatttcctcc atagctagct ctttgatcgg tacttttttc ggtaatgcat ggatgatccg
    10561 tgcgccatat tttgcagtga ctagtcttcg atccaaaata agcaatacgc cacgatctgt
    10621 ctccgtcctt atcaagcgac ctaatgcttg gcgtaacttc aaagctgctt taggtacgga
    10681 ttcctggtaa aaaggattga tcccctctga ttctaaataa gcgttcctag ctttgaccat
    10741 cgggcgaagc ggattatcga agggtaaacg tgtgactaca acgatctgca aagcatctcc
    10801 aggcaaatct acaccttccc aaaagctgtc agcgccaaac aatacgcttt tttctgacaa
    10861 aatgaagcgt ttcaaaagct tctcccggct tccaccaaac ccttgagcta gtatttctcg
    10921 tcctttttct agcatagaca gatgcatttt ctgatagacc ttctgcaaaa catcatgaga
    10981 agtgaataac acaagtattg gccgatcttc ttgttcgatc agacctgtaa gtgctgtctc
    11041 taaataatcg atatatgctt gattgctgac ttgatggacg gcaggtgcct cttttggcac
    11101 atagatcctt gcctgtttct cataatcata aggagaggca atcactttaa gaggtgctgc
    11161 ttcaatgcca agcctcttag agagatagtg tcgattaggg ccgatcttca gcgttcctcc
    11221 aatataaaga atacgatcat atcgaggata ccaaacagtc gacggcaaca tagccgcttg
    11281 aaaatcatgg agatgaaaaa tcatctgctg cgctgtgttc ccaggaacga accaatggat
    11341 caaatgatct cgccagtctt ccagccattg atccatcagc aagtattgct tgtctaattg
    11401 atcaaatagg ctatataaac ttgctaaatc gatccgcatc tggtttgtcc agctttcttg
    11461 gataatctca aaagtcagac gcatttgtga ctgtagctgc tggatctcct gataaaaaag
    11521 ctgcaggtga tcaatggctg tcccagattc cagtggcagc tgatccaaca tctcttttgt
    11581 catcatgact tcttctggat actgtccgtt aggttgcttg ccgctcacta aactcattaa
    11641 tccttctgcc atacaaatct gtgcttcgca tagcgcagaa agttcttctc gataaatcga
    11701 taaccagcgt aaggcttcat gttctttcgg taaatagctt tgtattcggt caaacagctg
    11761 atttggttct tctaattgct ggatttgttt atggaaatag ttaatactta atcgtttgtc
    11821 atttactttt tcagcgattt ccggcaaatg atgtacttca tcgatcaaca aaaatggact
    11881 agctggaagc aatggttctt ttcgctgtgt ttcttgtact aaaaacgcat gattgacaat
    11941 caaaaaatta ctttgcctga ttttcttttg caagaaacga acaaagtctt gttcgtaaaa
    12001 tgactgattt cttgctaaaa atccaatacc tctgtgcgcg atatctgcaa agaatgaatg
    12061 gttcaaattt gttaaatgta attcatccaa atcaccagtc ttcgtttgcg tcagccaaac
    12121 taagaccgcc atttgataca aagcatattg tttttgttct ggcggttcca caagtgtgcc
    12181 tttaaaacgc tgcaaatcaa tatagtggcg atagcttttg ataattacag gttgtatggg
    12241 ctgatctagc aattgattca gcaatggaat gtctttttct atgatctgct gctgcaaaac
    12301 tagcgaaacg gtactgatga cagcgggttt ttcaggtgta gccaaataac tcaatggcag
    12361 cagataaccg attgttttac ccattcctgt agctgcttcg atcatcaaat cttttgattc
    12421 atcttttgtg aaatggtcgt agaccgcatt catcagtctg ttctgttctt ttcgatacat
    12481 cagtgtttta ccaaacattt tttcttttgc ttgttttttt cttggatatg tccgctcgcc
    12541 ataatggacg gatgtgaata gttccacttc ttttttctgc aaagctagcc catcgacgat
    12601 ttctaatgag ggatcaagtg gctctggttt ttctttcatt tcttcaatca catgataaat
    12661 gaaacgactc gtatccatac ctgtgtgtct acttaatcgc gcaatctgct ctaacgtgat
    12721 gattggcaat tttctcatcc gttcttcgat cagcaaaaga agttgacctg tcacttcggc
    12781 atcactatct gcctgatgag gattctcatg aataaatcca aggctttctg acaaatcagc
    12841 taagcgaaag cttggttccg tcggcaagaa aatttgtgca agttctactg tatcgatgcc
    12901 tggtatcttc aaactaggaa gtccgcatcg catgagttca tggttcaaaa aattataatc
    12961 aaaataaata ttatgcgcga caaatatggt gtctgccaaa agattgtaaa tcgtttgggc
    13021 gacatcttcg aaataaggcg ctttttggac gcgttgattc gtgatccctg ttaaatgctg
    13081 gatttgtttt gaaatcttcc gccctggatg aatatcgatc gaaaaacgtg aagtgatccg
    13141 acctgattca atcatgacgc agccaaattg gatgatccga tcttcttttg gattcgtccc
    13201 agtcgtttcg atatcaacga ctgcataagt tcttggtttc aaaatatctt cctcttctcc
    13261 tgcctctaat gttcttttga aattatacta caaagtgcag aaaaatactc tcatcgcttt
    13321 ttaaagaaaa tagacaaaaa cgcccagaca cgcaaatgtc tgggcgttta ttttttagta
    13381 taggttaatt aagctttagt aacgtttgtt gcttgaggtc cgcgttggcc ttcttcaaca
    13441 tcgaaagtta ctgcttggcc ttcttctaaa gttttgaatc cgtcgccttg gatagctgag
    13501 aaatgaacga atacatcgtt tccgttttct gcagtgatga atccaaatcc tttttctgag
    13561 ttaaaccatt ttactgtacc tgtttccata ataaaaatcc tccttgtgta tcaacacata
    13621 ttttgcaatt acttgaatgg tgtatatctg gaagatgttt atttgaaaca agctaccaca
    13681 gattaccgaa caaaaactac tcctatagta tatcataccg tttacaaaaa agctagagtt
    13741 tttcgacttt ttttattaaa aaaagttttc tcagcgatta cataaagtga aaaaccgaac
    13801 ttttttttaa aataaacgac atattgttca gttggtgttt ttttgctctg atgttctttt
    13861 ttaaactaaa aaaattattc tttctgacta cttatcggtt ctgtttcaac ggtcgaggct
    13921 ggggaagatt ctttagtagg tctgatgttc tcatggtatt tctgatagcc tatgacagtt
    13981 accagaagaa caaggcaaac caatacgacc aacgagatta gtttttcttt tttgctcata
    14041 tctatacctc ataaataaca ggatgatgtc catcatattt ctgagtatga gtaaaagcaa
    14101 aagctgcagc tgtagaatgt aagaaatgcc agcaactttc ataatcacag gtcggacagt
    14161 accaaaagta accaaatctt ggctgcaaag accgttcttc tgtttcagga atcgcagaat
    14221 caagatctgg tgtctgtaca taaacatttt tgtacagacg tgtgcctgta ataggaaacc
    14281 gctgtgtttc ttcagcaaaa actggctgac ttgttaaact tagggataga gacagcaaac
    14341 accataacag tctttttttc ataaagacct ccttatcgat aagtttttcc caattaaaat
    14401 catatctcaa gaacgcccgt tattcacatt ttgttcataa atgatgtttt ttgtatcata
    14461 atcgtcaaaa agaggtcgtg accacgtcac aacctctttt ttaaacggtg ttccagaagt
    14521 agcttcttcg gaaataagcc gaaatttcac aaaaatttga gaaataattt ttgtcaatct
    14581 cttcttattt cttgaagctg accacttctg tcacaacctc ttccttaaac ggtgttcaaa
    14641 agcttgcatc tgcggtaata agcccaccaa acaaaaaata tgaagagcta tttttgcttg
    14701 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctcatacac ggtgtttaat
    14761 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gtgaaactat ttttccgttt
    14821 gtttctctaa tactcaggtc aaaacactga tttcacaacc tcttttaata acgaagaatc
    14881 gctgtcagac tcttttcatc tggcgcatct ttttgatcca aaataaacac ttttccgcca
    14941 ttaatgatca catcatctgc taatgcattc aacacttgac gccgatcata ttctgtttca
    15001 ggatcttcac cgaaaccatc agctaagttt gacgtagcga taaataaatg tgacactttt
    15061 ccttctttcg cagctggaac gatatcaact aactgatcga tgaatttctt atctaagagt
    15121 ttattgtatt cttttgattc ttgagctgcg agttcacttg acagcttctt cgccccttct
    15181 tcgatatctc taaaggatag ctgcgcagga gaagcagaaa tcgcaacgtc tgcacaataa
    15241 tagggatttt tagcaatttt tttgaacatc gtctgatttt ctggtagagc aaataaataa
    15301 atcggccatt gttctggatt ttcgaaagaa tccttcaagt acgtatcaac tgcttggtaa
    15361 tagttgaccc aatcgatctc tacttcttca tcttttggat ttacgccatg gaaagttact
    15421 ccatctcggg ctccgcctcc tccattagaa gagaagttca aaccgccacc tgtcagttca
    15481 gatcccaatg cagtaacgac atcaaccggt gccccttctg gtagagatac ttcttttact
    15541 tgtttgttgc tgacttcata caatttcatg gagtcgcggt tcaatcccaa taaataataa
    15601 gaatagttga actgcccatt tttgataata gccagtaaat aaggaagatc acttacatag
    15661 tattgatcgt ctaccgggat actcaaacga tggatatagg tttggtcagc agttataatg
    15721 accgctacac tcgtagttgc actacgccaa aacgaaggat ctcctaacaa attttcaatt
    15781 ttttcttgga acggggacca gtcatgttcg gaatattttt tctcaaaacg tttctttgct
    15841 tctttcgcaa aatttttgaa tttgatctga tctttttcca catcttgatg ggcgacatgc
    15901 gtattcagca tgatcgtgac gaatggtcct ttagcgcctt ctgaagtcaa ttgtgacaat
    15961 aattctttat ccttttttgc cataaacgta cccctcctaa aaaaatcatt cctttgtttc
    16021 tatctaaagt ctaccacaac actatttttc catacaataa aacgtatcta aaaaaataaa
    16081 acggtttcta cggttttaaa tttagctatt tttttaagtt atcttcataa tttcctcaat
    16141 tatttagccc cagtcttcaa ctattcttca tgaaatgctg ataaactaag aaaaagaata
    16201 aaagaggtgg atcttatgca tttgattatg attttcagtt tattaagtat tatcagttta
    16261 tttgctttca gcgcttcccg cttattagat gcggaaacac agcctattcc tgtaaaagta
    16321 cgtaaaaacg accgctaacc ttccataacg tgcgttctta tcctaaagtt ttttcagaaa
    16381 cattgaaact ctctcccctc tctgataaaa ttttaatttg tagcgaaaca aattaattac
    16441 aaggagatcg gatatgtcca taaaaaaaac acgtctagtc ctattattgg ctagtctcat
    16501 tctttgcctt gcttcttttc taccagctgt tgctgtgcaa gctgaagata ctttcaaagt
    16561 aaatgcaaaa gcagcctttg ctgtagatgc gcaaagcggt aagattcttt atgatcaaga
    16621 tggagaaaaa accatgggga tcgcttcaat cacaaaaatc atcggtcttt acatcgtcct
    16681 tgatcaagta aaagaaggga aactatcatg ggatgataaa gtttcaattt cagattatgc
    16741 agaaaatttg agtgtcaccc cagatttatc aaatgttcct ttgcataaag aaaacaccta
    16801 caccgttaaa gaattattcg actctgcaat catccagtca gccaacgctt caatggttgc
    16861 cttggcagaa aaaatctctg gcagcgaagc aaaattcact gaacgaatga aagaacaatt
    16921 gaaagattgg ggtatcaaag atgcaacgat cgttaatgca tcaggactga ataactctta
    16981 tcttggcgaa aaccgaccag aaggtactgg agaaaacgac gaaaatcaaa tgtctgctca
    17041 agatgtggct atcgttgccc gtcatttgat cttggatttt ccagaaatac tggatgtatc
    17101 tagcactacg acacaaatgt ttggcgaaaa cacgcaatca ccagtagaaa tggtcaactg
    17161 gaactggatg ctcccaggat tcgtgaatta taaagaaggc gtggacggat taaaaactgg
    17221 tacgactgaa cttgcaggag cctgtttcgt cggtacgatc actaaaaacg atcaacgaat
    17281 catcactgtc gtattgaatg cagacggcca tgcggaaaac ccaagtgccc gtttcaatga
    17341 aacagctcgc ttaatggatt attgctacga caattggtca gaaaaagagc ttggaaaagc
    17401 caatgcaagt atccctagtc taaaaaccat tgatgtaaaa gacggaaaag aatcaacagt
    17461 gcccgttgtg cttaaaagtc ctgtaaaagt atgggtacgg aacgatatgg atactggaaa
    17521 attaacaatc acaccaactc ttgacaaaaa acaagtcact gacaatgaga tccaagcacc
    17581 tatggataaa ggaacaaaaa tcggtaccgc aacgatcacc ttggcagaag ataaattagg
    17641 ttatcttgaa gacaacaacg cgccatccac agaaatcatc acggataaaa ccgtagaaaa
    17701 agcaaatatt ttcgttattg gctggcgacg cgtcgcagaa tttttcggta acttgtttta
    17761 attgaacaaa aagaaaaaag catctttcca tgtggaagat gtttttttgc tttttctagt
    17821 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    17881 tatgaatcta accagagatc tgattttttt ggttctctat cttattgggg tattctccat
    17941 cgtttattgg cgaaaacaga aaaaggatcc ttccttgttc ttttacagca gtacatggac
    18001 gtttgcggta atcttgactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    18061 tttgattccc ttagtgtttt tcagtatctt tgccttttcc tatttttcag aaaagcgaag
    18121 gctgttgaat ggtcttttat tcaatatttt cctcattagt tttggtatct acttgtttgt
    18181 cttgctatac gaaacacaaa atatttttct tggcggactg attgccttga tcacgatccc
    18241 gttactgctt gtctttctat tcggcattta tggattgatc gtttttcttt tttggaacgg
    18301 tgtcacagtt ttacgtagag agtcgcattc attagctaat cttttaacgt tgatactagc
    18361 catttttctt acattgtttc ttgtttttga ctttttcttg ttaaaatatt taccacaatg
    18421 gataaatgct ttgtttttct gcatcccatt gatcttgatc tatctattta tcgtattcta
    18481 caactttttg actgtctcat tcttgtatca gttcaaccgc cctcgataca accaagactt
    18541 catcgttgtg ttaggcgccg ggttgatcaa tggagaaaca gtttctcctc ttcttgccaa
    18601 gcgaatcaat aaggcgattg ctttttattg ggcacaaagc cgtgcgacat taaatccgcc
    18661 tatcctatta atgtcgggtg gacagggagc agacgaaaaa gtaccagaag caatggcgat
    18721 gaaacaatat gccatggaac aaggaattcc tgaaagagat attcttgtcg aaaccaattc
    18781 gacaaccact ttggagaata tgcgctattc aaaggaaatc atggatcagc aaatgaaagg
    18841 tccctatcgc gcgatcttca gttccaacaa ttaccacatt ttcagagctg gtctctatgc
    18901 acgacaagcg aaactgaagg caaatggcat tggggcaaaa acagctttct attacttgcc
    18961 taacgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    19021 catttgcgga ttgcttttcg caggttccgc attcttatca ctgatcactt tacttttcta
    19081 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac
    19141 tattggcaaa aagagtatcc cttttctgac cgctacttgc tgagcagaat aaataccgct
    19201 gttgcctgaa cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga
    19261 accgaacaat tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa
    19321 tactccgata aagcccaatc ctgcaagaaa aggaacagaa acatggagca aaggcgctag
    19381 tgctgcacca agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc
    19441 cgcgcctaaa gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg
    19501 tccattaaat gattcctgca gtaatggaag actcaatccg agataacgtt gtgtttgcaa
    19561 aatcaatgct gcggctacaa ctacagcccc acctatcgta tttcgccaaa caggatgagg
    19621 aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
    19681 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
    19741 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca taaaatttgc
    19801 aaacagtgca gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa
    19861 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
    19921 actgattcca ctgataatca agatttcccg ttctgattta tccaatcgga acaaacgtgc
    19981 tgtattatct gctaacgctc cccccatttg gactgctgtt ccttcacggc caacagatcc
    20041 tccaaataaa tgggtagtta tggttccaaa cagtgtaagt ggaataagtc gcaacgggat
    20101 tttttcttct ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagacc
    20161 gccgtgatac gcatagagat aagcaaaaag cgcaccacta actggtagta gataaaggag
    20221 ccaaggatgt gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga
    20281 taagctcccc atgaatatac cgagaatcgc tgtgatcacc agccacttca gcatgtacac
    20341 taaaatgcgt actggactcg gtaattgttt ttccataaaa aattcctcct caataaaaaa
//
LOCUS       DAYYCQ010000050        20287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000050 DAYYCQ010000000
VERSION     DAYYCQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.050"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1648)
                     /locus_tag="ACHAYF_001797"
     CDS             complement(89..1648)
                     /locus_tag="ACHAYF_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8579236.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1803..4037
                     /gene="pcrA"
                     /locus_tag="ACHAYF_001798"
     CDS             1803..4037
                     /gene="pcrA"
                     /locus_tag="ACHAYF_001798"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8579237.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4054..6090
                     /gene="ligA"
                     /locus_tag="ACHAYF_001799"
     CDS             4054..6090
                     /gene="ligA"
                     /locus_tag="ACHAYF_001799"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8579238.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6196..6501
                     /gene="gatC"
                     /locus_tag="ACHAYF_001800"
     CDS             6196..6501
                     /gene="gatC"
                     /locus_tag="ACHAYF_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8579239.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6501..7973
                     /gene="gatA"
                     /locus_tag="ACHAYF_001801"
     CDS             6501..7973
                     /gene="gatA"
                     /locus_tag="ACHAYF_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8579240.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7970..9400
                     /gene="gatB"
                     /locus_tag="ACHAYF_001802"
     CDS             7970..9400
                     /gene="gatB"
                     /locus_tag="ACHAYF_001802"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8579241.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9410..10465
                     /locus_tag="ACHAYF_001803"
     CDS             9410..10465
                     /locus_tag="ACHAYF_001803"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8579242.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10576..11949
                     /gene="rlmD"
                     /locus_tag="ACHAYF_001804"
     CDS             10576..11949
                     /gene="rlmD"
                     /locus_tag="ACHAYF_001804"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8579243.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11952..12080
                     /locus_tag="ACHAYF_001805"
     CDS             11952..12080
                     /locus_tag="ACHAYF_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8579244.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12520..12708
                     /locus_tag="ACHAYF_001806"
     CDS             12520..12708
                     /locus_tag="ACHAYF_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8579245.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12786..13799
                     /locus_tag="ACHAYF_001807"
     CDS             12786..13799
                     /locus_tag="ACHAYF_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8579246.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13866..14900)
                     /locus_tag="ACHAYF_001808"
     CDS             complement(13866..14900)
                     /locus_tag="ACHAYF_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8579247.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15015..17240
                     /locus_tag="ACHAYF_001809"
     CDS             15015..17240
                     /locus_tag="ACHAYF_001809"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8579248.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17258..19141
                     /locus_tag="ACHAYF_001810"
     CDS             17258..19141
                     /locus_tag="ACHAYF_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8579249.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19122..20096
                     /locus_tag="ACHAYF_001811"
     CDS             19122..20096
                     /locus_tag="ACHAYF_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8579250.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6838 a         3720 c         4343 g         5386 t
ORIGIN      
        1 ttttcccagc ctcttctttt tttatagaat catatagaat caagtagtgt agatgaatag
       61 ttgttgtaaa atagtttttc catgttcgtt aaaatgattg cttcaaattt gtgatagaag
      121 atttagcctc gatttcctgc ttgattcttg gcaagtttgc ttcatcagtc aagataacca
      181 aaatatctcc tacattcatg atcgtatcac cgtgagtcaa gatttcctga tttcctctgc
      241 gtatagagat tagcagcata tttttaggcc aattgaagtc tcgtaccatt aatccgtcta
      301 aagaactctc tgccgttact ggaaaatcaa aagttgtcct tgcacctttt agtgaggcgg
      361 tctgagtatt tccaagtaga cgttccaata atgattcgta aattggatta ccgccgagaa
      421 ggtcattgac gatataagca acaagagcag ccaatcctaa aggcatcaga tgattaagag
      481 agccaaccat ttctgtcact aaaataattg ctgtcagcgg tgcttttcca atcgctgtga
      541 aataaccagc catggcaata acgacaaaat ccttgatgta gattggatcc atcccgaaat
      601 caaccataat gtttccataa aagctaccga ttaaagcccc caatgtcaat atcggaagga
      661 aaatcccccc tggaagaccc ccaccgtaag aaatcatgga aaaaacgaat cgaagaacaa
      721 acaaaccgat aatcataatg attggcaagg aagcatgtcc aaaagctaaa acgatttgat
      781 tgcctccgcc tagagtacta ggccataaat aaccaattgg caaaatcagc aagaaaggaa
      841 cgaacccgtg aaaataagct ggtaaaggca atcggttgta aaattttggc aaagccagca
      901 agacattctg ataaataaaa cctaagatgc ctagaagtac tcctaagatc actaaggaac
      961 cataatattt caacggcaaa gaaggaaggt cacctagata taaaacaggt ctcaagccga
     1021 aaaagtaaag agagacgaag ttagcagtga ttgctgaagc aaatgatgtc agccaaacaa
     1081 gtggagaaaa ggagtgatgg atttcttcta aaacaaagag taatcctgcg ataggagcat
     1141 tgaatgctgc tgcaagtccg ctacttgcac cgctagaaat taaaatcttt tcttctgaag
     1201 atggactccg aaacaattgt ccagctcctt gtccaatgga tgcgccaagc tgtatagaag
     1261 gtccttctct acctagaaaa aggcccgcac caactgaaag tacaccgcca atgaactttt
     1321 tccataaaac agagaaccag ttcattgata attccccgcg aagctgtccc tctacttgtg
     1381 gaatcccgct tcccttgata tccggttcat ttttgaccag ataacccacg aaaaatgctg
     1441 caaggatgca gcaaatagct aggccaatta agtaaatcgg ctgttcccga ctcatttcat
     1501 agatacttcg aactaaatcg cttagctttt cgatagataa tcgaaacagg ctaacgacga
     1561 ttcctgtcag cagtccaaca aatattcctt ttagaataaa atatatctga gtcttatcta
     1621 atttcttgat ttttcctttt ttttccattc aaacgcctcg ctttctttta atttagcgcg
     1681 aaatcaacga attgacaagg aaaaatccat aaaaagaaac aaatgatttt gaactagcta
     1741 gtttaatgaa agccaagaaa atgctataat taacaagatt gataaaagaa aggatgttgt
     1801 taatgtcatc caaagctgaa ttattaaacg ggatgaaccc ccgtcagaaa gaagctgttc
     1861 ttcacactga cggaccatta ctgctaatgg caggtgcagg aagtggaaag acgagagtgc
     1921 tgacacaccg cattgcctat ttgattgagg aaaaagaagt caatccttgg aacatcctag
     1981 cgattacttt taccaataag gctgcaaaag aaatgaaaga acgtgtcaat gcgattctcg
     2041 catctggagg cgaagatgtc tgggtatcga cttttcactc gatgtgtgtg cgtattttaa
     2101 gaagagatgt tgattttatc gggtataacc ggaactttac aatcatcgat tcttctgaac
     2161 agctgacctt gatgaaacgg atactaaaag aactgaacat tgatccgaaa aaatatgatc
     2221 ctcgaagtat attaggaaca atctctcaag caaaaaacag cctgcagacc cctcaggatt
     2281 ttgcaaaaat gcaaggcagt tattacgaag agattgcggc aaaatgttat gctgcttacc
     2341 aaaaagagct tcaatataat caatgtatgg actttgacga cctgatcatg aatacgattc
     2401 gattatttga agaacatcct gattcactca cgtattatca aaacaaattc cattatattc
     2461 atgtagatga gtaccaagat acaaatcacg cacagtatac gctagtcaat ttattggcag
     2521 gtagatttag gaacctgtgc gtcgtgggag atgcagatca aagtatttac ggctggcgcg
     2581 gtgcagatat gcagaacatc ttggattttg aaaaagatta tccagatgct gcagtaattt
     2641 tgctagaaca aaactatcgc tccacgaaaa acatccttag cgcagcaaac caagtcattg
     2701 aaaacaatag caatcgaaaa ccaaaaaact tgtggacaga aaacaaagaa ggaaacaaga
     2761 tcacttacta ccgtgcagat aatgaacgag acgaaacccg ttttatcgta gaccggatgc
     2821 aagaagagat tcgaagcaat catcggaatt acggcgactt tgccatcttg taccggacga
     2881 acgcacaatc tcgtgtaatg gaagaaactc tgttgaaagc aaatattcct tataaaatgg
     2941 ttggagggca caagttctac gatcgtaaag aaatcaaaga tattttagcg tatttgaatg
     3001 tgctggtaaa tcctcaagat tcaatcagtt ttgagcgtat cgttaattcg cccaaacgag
     3061 gcatcggacc aggatctatc gaaaaactac gcagttttgc ttctcttcat gaatggccac
     3121 tgcttgaagc agcgcaaaat gtggatctgg caaatatcgg tggaaaagca ggacagcaat
     3181 taggtgcatt tggagagatg atccaagaag tcactcagat gatcccatac ttgacagtga
     3241 cagaattgac gaaagaggtt ttggatcgta gcggatatct agaagactta aaaatccaaa
     3301 atacattaga agcacaagca cgcatcgaaa acttggagga atttttaacg gtcacacaag
     3361 aattcgacaa acaattcgaa cagcaaaatg aagaagacgc tgatgcacca gaagaaaaac
     3421 taacggtctt cttaaatgat ctggcacttg tttccgacat tgacaattta gaagaagatg
     3481 cttcccaagt gacattgatg actttgcatg cagccaaagg attagaattt ccagttgtat
     3541 tcctgattgg tctagaagag ggggtttttc ctttatctcg agcattgatg gaagaaagcg
     3601 aactggagga agaacgccgt ctcgcatatg tggggatcac gcgagcagaa gaagcgttgt
     3661 atttaacaaa tgctttctcc agaacattat atggacggac gcaatataac cgaccttctc
     3721 gttttgtaga agaaatcgat caggagttac tggaaatcga aggcatgcgt cctacgccta
     3781 aaaaaacgcc tgtattcgct aaaaaaacag catatagcta taaacagcca gagacagcgg
     3841 ttgttccctc taaaagtgct actggagggg aaaataacag ctggaaacct ggagataaag
     3901 tcaaacacaa aaaatggggt ctaggaacag tcgtccgagt aagcgggacg tcgaaggatc
     3961 tagaactaga tgtggctttc ccaagtcaag gagtaaaacg tcttttggct gcctttgcgc
     4021 caattgaaaa agcttagtag tggagggtaa atgatggaac atgaaatgac gattgacgaa
     4081 gctgctaaac gagcagaaga acttcgtaca agactgaacc aatggtctcg cgaatactac
     4141 gtagaagata aaccaacagt ggaagactat gtttatgata aagaatacgc agaattagta
     4201 gcaatcgagg aacaatatcc tgatttgatc acgtctgatt ctcccactca acgagtaggc
     4261 ggaaaagtat tagaaggttt tgaaaaagtc actcatgata ttccgcttta cagtttgaac
     4321 gacgttttca gcaaagaaga gctgatagct tttgatcaac gtgtacaaaa agcagtggga
     4381 cgtgtagttg actattgctg tgaactaaaa atagatggac tttccgtttc tttgcgttat
     4441 gaagatggaa atttcgtgag aggtgcgact cgcggggatg gaactgtagg agaaaacatc
     4501 actgaaaacc taaaaactgt ccggtcggtc ccaatcaaat taaaagaacc aatgaacatc
     4561 gaagtaagag gcgaatgttt catgccaaaa cggtcatttg tccaattgaa ccaagacaga
     4621 gaagcagagg gaaaagatat ctttgcgaat cctcggaacg ccgcagctgg ttctttgcgt
     4681 cagttagatt ctaaaatcac agcgaaaaga aatctggaca cttttttata cacagtagct
     4741 gatttcggtc ctatgcaagc aaaaacccag tatgatgcgc tagaagagct agaaaaaatt
     4801 ggattccaca cgaatcggga aaaacgtcta tgtcattcga ttgatgaagt gtggtcatac
     4861 attgaagaat atcatgacaa acgtgtggat ttgccctatg aaatcgatgg aatcgtcatc
     4921 aaagtgaatg aattctcgct acaagatcaa cttggtttta ctatcaaagc accgagatgg
     4981 gcagcagctt acaagtttcc gccagaagaa gttgaaacgc taatcgaaaa catcgaatgg
     5041 acagtcggtc gaacaggggt agtaacgcca acagctatca tgacaccagt ccgagtagca
     5101 ggaacgacag taagccgtgc cagcctgcat aacggtgatt atattaagtt gaaggatatc
     5161 cgcttgaaag atacagttct gatttataaa gcgggagaca tcatcccaga agtttcgcaa
     5221 gtcgtgttag ataaacgacc taaagacagc gaagaatacc aattgcctac acattgtcca
     5281 gtttgtggaa gtgaacttgt ccatttggat gaagaagtag ccttacggtg catcaatcct
     5341 aaatgccctg ctcaaatgaa agaaggtttg aaccactttg tttcaagaaa tgcgatgaat
     5401 atcgatgggt tggggccgcg cgtactagaa cagatgtatg ataaaaaact agtagcagat
     5461 gttgctgatc tttacaaatt gacagaagaa gagttattga cgttggacaa aataaaagaa
     5521 aaatcagcaa ataatatttt gacagcaatc gacaacagca aagataattc ggtcgagcgg
     5581 ttgattttcg ggttaggaat ccgacatgtc ggagcgaaag cagcaaaaat tttagcagaa
     5641 catttcggtg atctagaaac attgagtaaa agtgactatg agtcgattat tgcgcttgat
     5701 acgatcggtg atatcattgc cgacagtgta gtcacttatt tcagtaacga agaagtccat
     5761 gaattgatga atgaattaaa acaagcaggc gtcaatttcg aatacaaagg gcttcgaaat
     5821 gcccaattgc aagaagtcga gtctcctttt aaagaaaaaa cagtagtcct tactggtaaa
     5881 ctaacacgat ttactcgtga agaagcaaaa gagacgatcg aaaatcttgg cgggaaagtg
     5941 actggtagcg tatcgaaaaa aacggatatc gtggtagcag gagaagatgc tggcagcaag
     6001 ctgaccaaag cacaagaatt aggaatcgaa gtttggacag aagatcaaat ggcagatgca
     6061 ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt ttttcagaca aatttcaata
     6121 aacaatttca gacacggcac tcttgtgtta aaatagagag caaagttttt aactcgttta
     6181 gaaagaaggg gtagtatggc gattagtgaa gaacaagtga aacacgttgc caaattagca
     6241 aaacttgcgt tcgcagatga agaacttgca agttttaccg accaattagg taaaatcatc
     6301 gatatggttg aaatgctgga agaagtagat acggaaggtg ttccatttac gtcgaatgtg
     6361 gttgaaacag taaacgtgat gcgacaagat cgggcgcaag ctggatggaa ccgcgatgaa
     6421 ttattgaaaa atgtgccgga aacagaagac ggctttatca aagtacccgc aatcatcgat
     6481 aatggggagg ctggcgcata atgacagaat tacacaatcg ttctctagaa gaactgcatc
     6541 atatgcttgt atcgaaagaa atcactgtac aagatttgac aaaaacaaca tttgaacgaa
     6601 tcaaagaaac agagcctgag gtcgaggcat tcattacatt gaacgaagaa aaagcattgg
     6661 aacaggcaaa agctttagat gaaaaaggga ttgatgaaag caacgtcctt gctggtattc
     6721 caattggtat caaggataat atcgtaacaa aagatatctt gacaaccgct gcatctaaga
     6781 tcctttacaa ttttgaacca atttatgatg caacggtcat ggataaaatc tatcaagcag
     6841 atatgatccc tgttggtaag ctaaacatgg atgaatttgc tatgggtgga agtacagaaa
     6901 cttcttattt caagaaaaca aaaaatgctt gggacccaac aaaagtacct ggtggctctt
     6961 ctggtggttc tgcagcagca gttgcagctg gacaaattcc agtttcttta ggtagtgata
     7021 ctgggggaag tatccgccaa ccagcagctt ttaacggcat cgtagggatg aaacctactt
     7081 atggccgtgt ctcacgtttt ggtttgattg cctttgcttc atctcttgac caaatcggac
     7141 caatgacccg tacggtgaaa gataatgcat tagcattgac agctatcagc ggttatgacg
     7201 aaaaagacgg tacttcttca ggcgtttcag ttcctgattt tgcagaggga ctgacaggcg
     7261 acatcaaagg aatgaagatt gctttaccta aagaatactt aggcgaaggt gtggcccctg
     7321 gcgtaaaaga agctgtattg aaagcagcag aaacattccg ttcattaggt gctgttgtgg
     7381 aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt ttattatatc attgcctcat
     7441 cagaagcaag ttccaatttg caacgattcg atggtatccg ctacggttat cgctctgaaa
     7501 atgtgaaatc attagatgat gtatatgtca actcacgttc agaaggattc ggtgaagaag
     7561 taaaacgccg tatcatgtta ggtacattct ccttaagcgc gggttattat gacgcttact
     7621 tcaaaaaagc aggacaagtc cgtacattga tcaaacgaga ttttgaaaaa gtctttgcag
     7681 attatgactt gatcatcggg ccatcttcac caacagttgc attcggaatc ggtgagaaca
     7741 ttaacgatcc aatcacaatg tacatgagtg acatcttaac gatccctgtc aacttggctg
     7801 gacttccagg tatgtctgta ccggcaggtt tatcagaagg attaccagtt ggattgcaga
     7861 tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc ggcatatgcg tttgaacaag
     7921 caacagaatt ccataaacaa caaccagcta ttttaggagg gaaagacgaa tgaattttga
     7981 aacagtcatt ggactagaag tccacgtcga gttaaaaacc aactcaaaaa ttttctctcc
     8041 agcgccagct cattttggtg cagaaccaaa cagcaataca aacgtgatcg actggggtta
     8101 tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa ttcgggatga aagcagcttt
     8161 ggcattaaac tgtgaaatct ctaaagatac tcattttgac cggaaaaatt acttttaccc
     8221 agataatcca aaagcatatc aaatttccca atttgatcaa ccaatcggtc atgacggatg
     8281 gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg atcgaacgtg tccatttaga
     8341 agaagatgct ggaaaaaaca tgcatggtat tggcggttat tcttacgttg atttgaaccg
     8401 acaaggcacg cctttgatcg aaatcgtttc tgaagctgat atgcgttcac cagaagaagc
     8461 atacgcttat ctagaagcat tgcgatcaat tatccaattt acagaagtca gcgatgtgaa
     8521 aatggaagaa ggctctatgc gctgtgatgc caatatttct cttcgccctt atggacaaga
     8581 agagttcggt acgaaagcag aattgaaaaa cttgaactct ttatctttcg tgaaaaaagg
     8641 tttagctttt gaagaaaaac gtcaagcaaa agttcttctt tcaggaggag aaatccaaca
     8701 agaaacacgc cgttttgacg aaacaacgaa taaaacagtt ttgatgcgtg tcaaagaagg
     8761 gtctagtgat tatcgttact tcccagagcc tgatatcccg aaattcgtga tcgatgacga
     8821 atggattcaa aaagtaaaag ctagtttgcc tgagatgcca gcttctcgtc gcgaacgcta
     8881 tatccgtgag tttggacttc cagagtatga cgcaatggta ttgacactta caaaagaaat
     8941 gtctgatttt tttgaagctg ttttggaaaa tggtgcagat gccaagcaag catctaactg
     9001 gttgatgggt gaagtttctg cttacttgaa cagtgagaaa ctggaattag ctgagacaaa
     9061 attgacacct gagaacttag caggaatgat tcgtttgatc gccgatggaa cgatcagctc
     9121 aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat ggcggagaag cccaagaagt
     9181 agttgaaaaa aatggttggg tgcaattatc tgatccgaac aaactactgc caatcattaa
     9241 tgaaattttg gataacaacc aacaatctgt cgatgatttc aaaaatggaa aagatcgtgc
     9301 ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa ggacaagcaa atcctggtgt
     9361 cgtgaataag ctacttaaag atgaattagc gaaacgatag aggctgaaaa tgaaaaaagc
     9421 acgcgtaatt tataacccca cctcgggaaa agaattattg aaaaaaaatt tggcggatat
     9481 cctacaggca ttagaagaag caggatttga agccagcgcc tatgcaacta caccagaacc
     9541 agattcagcc aagaatgaag catgtagagt cgcagaacta ggttttgatc tagtagtggc
     9601 agctggtggc gatggcacaa tcaacgaagt ggttaatgga atcgctccgc ttgaacaccg
     9661 tcctaaaatg gccatcatcc cagctggtac aacgaacgac tatgctcgag cattaaaaat
     9721 tcctagagat aatattaaag cagcagcaga agtgataaaa aaacaccaaa ccgtaaaaat
     9781 ggacatcggc ctttctgata acagttactt catcaatatc gctgcaggtg gctatttgac
     9841 agaattgact tatgaagttc catcagaact aaagagtatt tttggttatc ttgcttatct
     9901 tgcgaaagga gcagagatgc tccctcgagt aaagccaatc aaaatgcgct tgaagtatga
     9961 tgaaggggaa tatatcggaa atgcttcgat gtttttccta gggctgacga actctgttgg
    10021 aggattcgaa cagatcgcgc cagatgccaa attggatgat gggaagtttt ctttaatcat
    10081 tgtgaagaca gcaaatatct ttgagatctt gcatattgca gcactcatgc tgaatggcgg
    10141 tcgacatgtc gatgatccaa gaataatcta taccaaaaca agcagtttgt tcgttgaaac
    10201 agaagatgat atcaatcgac cagtaatgat caacttagat ggtgaatacg gcggggaagc
    10261 accgatgcat ttccaaaatc ttcatcaaca cattgagttc tttgccaata ccgatcagat
    10321 tcctgatgaa gcaatcacag gtacagatga agaagaatta gaggttgtca gcaaagaatt
    10381 cgtaaaagaa gtcgaacgtt taacggatga agatatcgac ggtgacggaa aaatagcaga
    10441 cgaaacacct aatcaaaaga attaatcaaa agccggtgct gaacgtagta aggtcagtgc
    10501 cggttttagt tgtgtaatta gctgatttat agtagagttc agtaggattg aaaaataggc
    10561 ttaaaggagc aaaatatgac agaacagcaa atagtaaaga aaaacgaacg atatacagtc
    10621 gaaatcgtcg acttgagtta tgaaggaatg ggggtagcaa aaatcgatgg ttatccatta
    10681 tttatcgaaa atgcactccc tggagaagaa gtagaaatac tagtagtaaa agcaggcaat
    10741 aagtttggat atggaaaagt cgaaacattt attacacaat cacctgatcg tcagcctgta
    10801 gaaaacaatg cattacttcg cataggtatc gcacctttag cccatttgaa gtatgaacaa
    10861 cagcttgtct tcaaacaaaa acaagtagag catgtaatgg cgaagattgc taagatgcca
    10921 gaagttcctg tacttccaac ggtcggaatg gcagaaccat tcggctaccg taataaagca
    10981 cagatccctg ttcgccgtat cgatggacag ttagccaccg gtttttacaa aaagaatagc
    11041 catgatctag tccctatcga agatttttat atccaagatc ctgtcatcga tcaagcaatc
    11101 aaagtagtca gagatatctt gcaacggttc caagtacgag gatataatga agaaaaaaat
    11161 gaaggacagc ttcgtcacat cattatccgt cgtggtcatt attcccatga aatgatggtc
    11221 gttttagtta ctcgtaaaga aaaattcttt aaatccaagg aaattgctga agctattcat
    11281 gaagaattgc cagaagtggt ttcggtcatc caaaatatca atgaagaaaa aacaaatgtg
    11341 attttaggag acaaagaaaa agttttatat ggcagagact atatcgaaga tcagctatta
    11401 ggaaaaacct atcgaatctc agcaaagtca ttttatcaag taaatactca acaagcagag
    11461 tatctgtacc aaacagccat cgagtttgcc gagttagata aaaaagacat tgtgatcgac
    11521 gcttattcag gaataggtac gattggcttg tctttagcag acagagtgga taaggtgtat
    11581 gggatggaag ttattccaga agcaattgaa gatgcacagt tcaatgcttt aaataataaa
    11641 atcgaaaatg ttcattatga agtcggaaaa gcagaaaaag ttatgaaaaa atggcaagaa
    11701 aaagggatca aaccatctgt gatcgtagtt gaccctccac gaaaaggatt agatcatcat
    11761 tttattgaat cagcaatcga aatgtcacca gaacgaatcg tctatatctc atgtaatccg
    11821 gcaacttttg caagagatgc taagttattt gcagaagcag gttatgccgt gaagaaagtt
    11881 cagcctgtag atcttttccc gcaaacccat catatcgaat tagtcgcttc attcgttaaa
    11941 ggagaataaa aatggaccaa aaagaaaaaa tgaaagaatt gatcaaaaag aaacaaggtg
    12001 gaggccgatc caaacaaatg gaaacaccta agaatgatcg taaaaacatg agaaaaggcc
    12061 ctaaaatctt taataaatag acgtgctaaa gaaagactaa gaagtgttaa cttttgttat
    12121 tgtttcttag tctttttagt tagtctacat tattttggcg atttgccaat aaggactgtt
    12181 gagttgtgca aaatcaaatc tgggtcagaa tgaacgtcat tctgtctcag atttttcgtt
    12241 atttcaattt gattaactga ttgatcaaaa agatttttat tttcatattc tcaaatgatt
    12301 caacactaaa tatacatgtt tgacgcggaa attattattc gattttggcg gcatcagggg
    12361 ggatgacgac gtagcagacc cattatttta ctcaagaaaa tgtccgtgcc tattatttag
    12421 aatacgatag tgaacgtgct gggagttttg ttcttttaga gaaagtctct gaagataaat
    12481 tagttgtctt agattttgac tgtaacgaaa aatggcgaaa tggaagagca ccagcaagtg
    12541 attgaccgga tctacgaagc gacacgatat ctgcctttgg atcgtttgtg cttaagtcct
    12601 caatgtggat ttgcttcaac agaagaagaa aatgttttgt ttgaagaaca atggaaaaaa
    12661 tttcttttgt caaaagcatt gcagaagaag tttggggaaa atcattaaat catctaggag
    12721 agcttgatag gaaataacac tatggtataa tcagaatgct taatactaac tactgaggga
    12781 gccttatgaa aaagaaaatt cttaaattta tcggatggtt cttcggaatc attcttggga
    12841 tcgcgtttgc aatctttctt gccttccagc tttcccccag acctggagca ttcttgatca
    12901 accatatgtt ttcaaatact gttcaaatca ctgataaaaa aacgttcgat caagcagaaa
    12961 aaaacgtggc gaaaaaaaca gatttgacgt atgagtccaa gtataaagag aatacttatg
    13021 acatctacta tccaaaaaac agcaaggggc ctgttcctgt tctattttgg gtacatggtg
    13081 gaggattcgt cggcggagat aaatcgggtg tcaaagaatt tgcgacaaaa ttagtttcag
    13141 acgccaacat cgcagtggtt gcgatgaatt atgaattagc gcctgattca gaatacccta
    13201 atcaagttct tcaagtgaat gaactgatca aagatttatt agctgaagaa aaagacgaca
    13261 aattgctaga catggaacaa ttattttttg gtggagatag tgcaggttca caaatcgcat
    13321 tgcaatatgc agctatacaa acgaatgatg cgtatgctaa acaaatgaaa atcaaacagc
    13381 tacttccaaa agatagtctg aaaggaacca tttcttattg cggacctgtt gatttgaagc
    13441 taacagcaca gcagcattcg gatgatcgat ttatgaagtt tttcgtcaaa accgttgcgt
    13501 ggtcgttgat tggaacaaaa gattggaaaa cgagtgagca attgaaagaa gctagtgtgg
    13561 ctgaccatgt ttctaaggaa tttccgccaa cgtatataac tgacggaaat gcatactcat
    13621 tccaggagca aggtcaagca ttagaaaatc ggttgacaga attaaaggta cccgtgcaat
    13681 ccttgtttta tacagataca aaaaaagaga ttacccatga ataccaattt gattacacgc
    13741 taaaagaatc gcaaaattgt taccagcaga cattggattt tgtgaataag tataagtaag
    13801 aggttgtgaa aaagccgttt cgttttgagt atgaagaaac gaagcggctt ttcacaacct
    13861 ctctttcaat acctttttgt tcttgggata aaactttccc taacaatcaa tcgtgcagga
    13921 taagtcaaaa tgattggttc gtcccgtata ccgtcaattc gctccaatgc cattcgaaca
    13981 gctgcttgcc caattgattt cgcattgatt tgaacactgg tcaagccagg ggagaggttt
    14041 gcgattgtct cgatattatc aaagcttgct acaacaatct cttctccaat ttggatattt
    14101 ttttgtttca atgcccgata gacaccaatt gccattggat cgctcccgac aagaatggca
    14161 gtaggaagag gggattcatt ttctaataga ttagtcgcaa gtacctcacc ttgtttttct
    14221 gtccagccac ctagatgata agacatatat ttttctaagt gatgatcgat cataaaatgc
    14281 tgataggcac gcatcctctt ttcatttata gaaatctgct tcccaccgaa ttcatccata
    14341 tcgactttat atcccccgat ataggctatt cttttatgac cattttctaa gaacaattcc
    14401 agtacggttt tagtcatccg ttcaagatct gcatacacca tatcgacctc ttgctggatg
    14461 tcagggttat ccacgacgac tatttgcatg ttttgtttta acaaggcggt aatcgattct
    14521 ttcgtaacag tcccaacaac gatcaccgca cctaactgat ctagatcttc ccattgttta
    14581 ggattatcag atagattata gattcgattc atccctaaat gcagacgtcg agcttcttct
    14641 tccatcccta ctcgcaaatg tttgtaatag atatcctgtt cttcgctcgc tttgccgatc
    14701 gtagagatca caccaatcga aggcattctc tttttgatat atttacgagt actgatgtca
    14761 taatccaacg cagcagctgc ctctactatc cgttgccgag tagtctgagc cacatggatc
    14821 gtctgatcgt tattcaacac acgggaaact gtagcaggag agacatttgc ctttcgtgcg
    14881 acatctctaa ttccaaccat ttttacacac tcctcaaaaa aataaaaaat ttttattgac
    14941 aaaattgttt agtaaacagt atacaggaat tataaaaaaa agtaaacggt ttccgttttg
    15001 ttgggagtga aatgatggat attacgcata gaaaaacaga taaaaatttc atcttatttg
    15061 atgaaaagag ccgagtcttc catttacgaa atacatttct ttcttatctg ataaagatag
    15121 aagaaagcaa tgtattggcg catatttatt ttggcaaacc agtcaagcag tacaaagaca
    15181 ataaaaatta tccccgaaga gatcgtggat tttcaggaaa tgttccctta aatcctgacc
    15241 ggtctctgtc gaaagataca ctaccgcaag aatactcttc tcatggcagc atggactttc
    15301 gaacgcccgc atcgatcatt cagcgcaaga atggatcaga cttactggat ctgagatatg
    15361 acagccatta tattacagat gggaagccag agatcgaagg cttgcctcaa acttatgtga
    15421 tggacaagtc agaggcacaa acgttggtga tttcacttaa ggatagagaa acagcgattt
    15481 atttcgattt gttctatacc atatttactg atagagcagt gatcacacga tctgtaaaga
    15541 taagaaatga aaccggagaa acaatcaaac tagaaaaagc ggctagtttc cagctagact
    15601 ttgcccatac taggcgattt gatgaagtca ttgctttgcc aggagcacat gtcaatgaaa
    15661 gacagatttc acggcaatct gttttatctg gaacaaaagt atttgaaagc cgtcgaggaa
    15721 cgagcagtca ccatatgaat aactttattg ctttagttca tcatcatacg acagaaaata
    15781 ctggagaagc gatcggactt caatttgttt attccggcaa ccattctttc gaacttgaaa
    15841 aagatcagat caatcaattg agagtcgtcg gtgggatcaa cagccatcgt ttttcgtggg
    15901 aattgaatgc aggccagtct tttcaaacac ctgaaatgat cctttcttac tcaagtcagg
    15961 gattgaacaa aatgagtcag atccaccatg agttgctacg tgaaagaatc gcaagaggtc
    16021 gtcatcaatt tgctgaacgg ccgattctgg tcaataactg ggaagccact tactttgact
    16081 tcaatagtga aaaaatcaag gcgatcattg atgaagcaaa ggagctaggc atcgaaatgt
    16141 tcgtattaga tgatggctgg tttggaaaaa gagatgcgga caattcttca ttaggagact
    16201 ggttcgagta tgaaggcaag ttgacaaatg gattacgtga aatagcagat tatgctcata
    16261 gcaaaggatt gaaatttggt ttatggtttg aacctgaaat gatctcggtt gattctgaac
    16321 tgtatcgaac acatcctgat tttcttatgc aggagccagg tagaatgcct tctgcttcca
    16381 gatcacaaca tgtactggat tttacaaggt tagatgttcg gcagacgatt gagaaacaaa
    16441 tgagaaagat tttagatacg attcctcttg actacatcaa atgggatatg aaccgttcgc
    16501 tttcagatgt ctattcgatt acccttgacc cacagagaca aggagaagta gcgcatcgtt
    16561 atatgctcgg gctttatgaa ctacttgaac atttgacaac tgattatccg gaaatcttat
    16621 gggaaggctg ttctggtgga ggtggacggt ttgatgccgg gtttatttat tatatgccgc
    16681 agtcatggac aagtgataac acggatgcag tggagcgtat gaaaatccaa tatggaacaa
    16741 gccttgctta tccgatttcg tccatcacag ctcatgtatc agctgtacca aatcatcaga
    16801 ccgacagatc tacttcgttg aaaacaagag gagaaacagc tatgagcgct gtttttggtt
    16861 atgaactgga tttgacaaag ctgtctcctg aagaaaaaaa gcaagtaaaa gaccaaatta
    16921 tatcctatca aacgattcgt ccggtgatcc agtatggcca ttattatcgt ttggcgagtc
    16981 cttttgaaga aaatattgcc gcatggatgt ttgtgtctcc taaacaagat gaagctattg
    17041 tgtttttagg gaggatacta gcttcagctc aaccagcgtt ccatgaagta tatctgatgg
    17101 ggttagatga tgaggcactt tatcaggaac agacctcgaa gcggatattt tcgggggccg
    17161 aattgatgac agttggactt tacttccccg attttcaagg tgatttccaa acagaactgc
    17221 ttcatttcaa aaagttatga gagagaagga aaaaagtatg aaagcaaata taaaatggtt
    17281 agatgatcca caagttttta gagtgaacca atcaaatgca catagcgatc atgtttgtta
    17341 tgcaacaagg gaagaagtag aaaccacatc ctcccttaaa cagtctttaa atggatcatg
    17401 gcgtttttgt tattcgccaa atccttcggt acgaccaaaa acattttata aaaaagattt
    17461 tgatgcctgt tcttttgatg aaatcactgt tcctagtcat attgaattag caaattatga
    17521 caagatccat tacatcaata cgatgtatcc gtgggaggga catgtatttc gccgtccccc
    17581 tttcacgaca agccctgaga aagaggcgga agggatcttt tcagctgcta aagacaatcc
    17641 ggttggttcg tatttgacga cttttcgatt agaagaaggt ctaaagggaa aacaaatctc
    17701 gattcatttt gctggtgtag aacaagcaat gtttatttgg ttgaatggtc attttatcgg
    17761 gtatgccgaa gattcgttca ctccgtctga atttgattta acaccttatt tggaagaaga
    17821 aaatattcta gctgtggagg tttacaaaag aagtacagct gcattcttgg aagatcagga
    17881 ctttttccga ttttttggga tcttcagaga tgtcttctta gttgctcgag gacaaaacca
    17941 tttagaagat ctttggatca aacctcgctt agatgacaaa tgtcaaaatg gcaaacttga
    18001 agtagatttg tcttttgagc aagtagccaa aaacagtcag ctgaatgtag cgatactaga
    18061 tcctgatggg aaggaaatct atcaacagca atatccgtta aaagagcaaa tgacgattcg
    18121 catcgatgga tttgaaaatg tggcactgtg ggggcatttt cagcccaatt tatataagct
    18181 tgaattggag acgatagacg aagaaaaaag agtaacggag tatattgact attccttcgg
    18241 ttttcgtaaa atcgaaatca aagataaaat catctacttc aatggaaaac ggatgattat
    18301 caacggggta aatcgacatg agtggcatcc gaaaaaagga cgcgccatca ctatagaaga
    18361 tatggaaaaa gatttggcta tcataaaaaa tgcaaagatc aacggcgtac ggacatctca
    18421 ttaccccaac cagatcactt ggtattattt atgcgaccaa gcaggaatcc atgtgatggc
    18481 ggaaacgaac ctcgaatccc atggctcatg gcaaaaaatg ggcgcatttg agccttctta
    18541 caacgtacca ggtgcagtac ctcagtggca ggcggctgtt cttgatcgag caaaaacgaa
    18601 tttcgaaaca ttaaaaaacc atccaagtat tcttttttgg tctcttggaa atgaatctta
    18661 tgcggaagaa aacattcgac aaatgaacga ttacttcaaa aaagtagacc ccgatcgact
    18721 tgtccattat gaagggaatt ttccaaatcc agcattcgaa gattcgattt cagatgtaaa
    18781 aagctgcatg tatgcgccac cagaagagat cattgactac cttgaaaata aagcggaaaa
    18841 accgttcatc ttatgtgaat atatgcatga catggggaat tcgttaggtg gaatggattc
    18901 ttacatgacg ttgcttgatc gttatgaaat gtaccaagga ggctatatat gggattttaa
    18961 agatcaggcg atccaagtga ctgatccaat cacaggcaga gaagtgatgc gctatggcgg
    19021 tgactttgac gataaaccaa gtgactatga attctcaggg aatgggatcg tttttgcaga
    19081 tggacaagaa aaacccgcca tgcaggaggt aagatattat tatgaaaaat acagtaaata
    19141 aaagtcatat ggatacggaa aaagttgcaa tcgtcttcgg cgactgtaca ttaggtgtca
    19201 aatcggggaa tacgcattat attttttctt atacaagagg cggactggaa tcgctcaata
    19261 aaaacggaaa agaatggcta taccgggaaa cacttccagt tttttggcga gcaacgaccg
    19321 ataatgacag aggtagcgga tttggattta aatccggtat gtggttagga gccggattgt
    19381 atccaaaagt caaagaaata gaagtactgg tggacaataa aaagatcgat ttgccgattg
    19441 ctccagtaaa taatcaatat tcaagtaatg aatacgctga aacggttgaa atcattttta
    19501 cactggaaat caacacagta ccaaaaaccc aagtgacaat ttcctaccaa atcgctggag
    19561 agggtaaaat gcttgtcaat atgcattatt atggacaaaa aggattaccg gaattacccg
    19621 cattgggttt aaggttcatt atgccaacga aagcaaaatt ttttgagtat gaaggcctat
    19681 caggggaaac atatccggat cggatggctg gtggtgtcga aggtatttat aaagtggcag
    19741 gactccccgt ttccccatac cttgttcccc aagaatgcgg catgcatatg gaaacgaaat
    19801 gggtagaact aacaagaaat caaacgttgg ataatacgga taaagatcaa gaagattttt
    19861 cgttgcgatt tgaacagcag gatacgccat ttgctttttc cgctttacca ttcaaagcag
    19921 aagaactgga acaggcaaca catatagaag aactaccgcc agaaagacga acggttctta
    19981 cgatttttgc aaaagtaaga ggagtcgggg gtatcaatag ctggggagcg gatgttgagt
    20041 caccttaccg aatcagcagc gaagaggacc atgaaatcgg ctttattatt cagtagcaat
    20101 ctttagatta ttatttgaaa aacggagttt acaagacaaa gctgctttat tctatatgtc
    20161 atgaacaaga gagtaaaaaa gttctcagat acttttgttc atgactattt tcttcaataa
    20221 aaccaattgt tatttttctg aaaaacaaag ttaactatca gaaaattaaa aaaagtttca
    20281 taaaaat
//
LOCUS       DAYYCQ010000051        19633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000051 DAYYCQ010000000
VERSION     DAYYCQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.051"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHAYF_001812"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHAYF_001812"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8579251.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHAYF_001813"
     CDS             complement(1172..1867)
                     /locus_tag="ACHAYF_001813"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8579252.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHAYF_001814"
     CDS             complement(1880..2596)
                     /locus_tag="ACHAYF_001814"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8579253.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHAYF_001815"
     CDS             2926..3534
                     /locus_tag="ACHAYF_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8579254.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHAYF_001816"
     CDS             3859..4182
                     /locus_tag="ACHAYF_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8579255.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHAYF_001817"
     CDS             4364..5734
                     /locus_tag="ACHAYF_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8579256.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHAYF_001818"
     CDS             complement(5864..7165)
                     /locus_tag="ACHAYF_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8579257.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHAYF_001819"
     CDS             complement(7519..9510)
                     /locus_tag="ACHAYF_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8579258.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHAYF_001820"
     CDS             complement(9654..9920)
                     /locus_tag="ACHAYF_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8579259.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHAYF_001821"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHAYF_001821"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8579260.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHAYF_001822"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHAYF_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8579261.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHAYF_001823"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHAYF_001823"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8579262.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHAYF_001824"
     CDS             complement(14080..14601)
                     /locus_tag="ACHAYF_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8579263.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHAYF_001825"
     CDS             complement(14604..15446)
                     /locus_tag="ACHAYF_001825"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8579264.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHAYF_001826"
     CDS             complement(15439..16164)
                     /locus_tag="ACHAYF_001826"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8579265.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHAYF_001827"
     CDS             16340..16636
                     /locus_tag="ACHAYF_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579266.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHAYF_001828"
     CDS             17204..18796
                     /locus_tag="ACHAYF_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8579267.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHAYF_001829"
     CDS             18809..19549
                     /locus_tag="ACHAYF_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8579268.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5620 a         4028 c         3724 g         6261 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaa
//
LOCUS       DAYYCQ010000052        19530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000052 DAYYCQ010000000
VERSION     DAYYCQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.052"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACHAYF_001830"
     CDS             127..3654
                     /locus_tag="ACHAYF_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8579269.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACHAYF_001831"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACHAYF_001831"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8579270.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACHAYF_001832"
     CDS             7442..7783
                     /locus_tag="ACHAYF_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8579271.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACHAYF_001833"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACHAYF_001833"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8579272.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACHAYF_001834"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACHAYF_001834"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8579273.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHAYF_001835"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHAYF_001835"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8579274.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACHAYF_001836"
     CDS             12994..13731
                     /locus_tag="ACHAYF_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8579275.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACHAYF_001837"
     CDS             13745..14560
                     /locus_tag="ACHAYF_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8579276.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACHAYF_001838"
     CDS             14573..15220
                     /locus_tag="ACHAYF_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8579277.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACHAYF_001839"
     CDS             15236..15892
                     /locus_tag="ACHAYF_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8579278.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACHAYF_001840"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACHAYF_001840"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8579279.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACHAYF_001841"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACHAYF_001841"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8579280.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACHAYF_001842"
     CDS             18234..18752
                     /locus_tag="ACHAYF_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8579281.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHAYF_001843"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHAYF_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8579282.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6569 a         3352 c         4190 g         5419 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga
//
LOCUS       DAYYCQ010000053        19451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000053 DAYYCQ010000000
VERSION     DAYYCQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.053"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..540
                     /locus_tag="ACHAYF_001844"
     CDS             91..540
                     /locus_tag="ACHAYF_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579283.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            540..1034
                     /locus_tag="ACHAYF_001845"
     CDS             540..1034
                     /locus_tag="ACHAYF_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579284.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            1048..1479
                     /locus_tag="ACHAYF_001846"
     CDS             1048..1479
                     /locus_tag="ACHAYF_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579285.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            1481..1618
                     /locus_tag="ACHAYF_001847"
     CDS             1481..1618
                     /locus_tag="ACHAYF_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8579286.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1655..1948
                     /locus_tag="ACHAYF_001848"
     CDS             1655..1948
                     /locus_tag="ACHAYF_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579287.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1945..2169
                     /locus_tag="ACHAYF_001849"
     CDS             1945..2169
                     /locus_tag="ACHAYF_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8579288.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2166..3185
                     /locus_tag="ACHAYF_001850"
     CDS             2166..3185
                     /locus_tag="ACHAYF_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8579289.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3374..3447
                     /locus_tag="ACHAYF_001851"
     tRNA            3374..3447
                     /locus_tag="ACHAYF_001851"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3408..3410,aa:Ile,seq:cat)
     gene            complement(3962..4261)
                     /locus_tag="ACHAYF_001852"
     CDS             complement(3962..4261)
                     /locus_tag="ACHAYF_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8579290.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(4267..4497)
                     /locus_tag="ACHAYF_001853"
     CDS             complement(4267..4497)
                     /locus_tag="ACHAYF_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579291.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(4933..5499)
                     /locus_tag="ACHAYF_001854"
     CDS             complement(4933..5499)
                     /locus_tag="ACHAYF_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8579292.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(5499..6455)
                     /locus_tag="ACHAYF_001855"
     CDS             complement(5499..6455)
                     /locus_tag="ACHAYF_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8579293.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(6455..7111)
                     /locus_tag="ACHAYF_001856"
     CDS             complement(6455..7111)
                     /locus_tag="ACHAYF_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8579294.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            7481..9499
                     /locus_tag="ACHAYF_001857"
     CDS             7481..9499
                     /locus_tag="ACHAYF_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8579295.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    9569..9775
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9926..12340
                     /gene="leuS"
                     /locus_tag="ACHAYF_001858"
     CDS             9926..12340
                     /gene="leuS"
                     /locus_tag="ACHAYF_001858"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8579296.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            12688..14241
                     /locus_tag="ACHAYF_001859"
     CDS             12688..14241
                     /locus_tag="ACHAYF_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8579297.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(14622..16406)
                     /locus_tag="ACHAYF_001860"
     CDS             complement(14622..16406)
                     /locus_tag="ACHAYF_001860"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579298.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(16421..18142)
                     /locus_tag="ACHAYF_001861"
     CDS             complement(16421..18142)
                     /locus_tag="ACHAYF_001861"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579299.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            18242..19003
                     /locus_tag="ACHAYF_001862"
     CDS             18242..19003
                     /locus_tag="ACHAYF_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8579300.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         6445 a         3472 c         3833 g         5701 t
ORIGIN      
        1 aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt
       61 acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc
      121 aatgtacagg tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg
      181 aagggagata aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt
      241 ggttcaaccg cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt
      301 ttaccaaagg gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca
      361 actacagcaa atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct
      421 ggagcacaga aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaacc
      481 gataaagtga aacttgatgg attagcgaat attacatttg agaaagtggg gacggtttaa
      541 tggctgatat tgttcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      601 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      661 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      721 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      781 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      841 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      901 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      961 acggagccat taaatggaga agaaattcaa gttatggatc agcttattca tttgttatca
     1021 cttattcaat ttaagggagg aaatctaatg aaagtagttt acaaatcaat caagccttac
     1081 ggattcgagc aaatcatttt gaacaatcaa gaaaatatcc ctgaaaactg tacagagatt
     1141 aaaccaccag ttcctaactg gagaccaaga tttgattttg ataaaaaaca gtgggttgag
     1201 ttagctactg aagaagagaa aaaagggaat gctgttgata gcgtagatga attggctaat
     1261 attaaggcat tatacgaaac gctgaaggca gaaaatgatg aattgaaaca gctaaattct
     1321 aaagcaatgc taaacaatgt agcaatcaag caagaaaatg tcttattgaa agaaaagtca
     1381 gaaagtttag cgcagttgaa ttcaaaaacg atgcttgctt ctgtacaaaa taccaaggaa
     1441 atcgaagaaa tcaaaaaaca attacaaggt gggaagtaac atgtattcat atgaagatat
     1501 caaactaatg tatgactggg gctgtttcac taacgaacaa gtaatggttt tcgttccatt
     1561 atgcattaca gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt
     1621 gatgcgtttt tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat
     1681 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc
     1741 tgcatctttc tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct
     1801 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
     1861 acacgatgaa agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa
     1921 aactttgttt agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc
     1981 gcactggtaa ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat
     2041 tggcagtaca cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt
     2101 gtgttgggtg tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga
     2161 aacaaatgaa aaagaaaatt actattactg cgatgagcct gttaacggct ctttttttat
     2221 tgccaattaa cggatttgcc tatactatca acaatgaatt taatttgggc gcaaatgaag
     2281 gtagctcaca agtagcaaat aatcagtata ttttactgca tgaaacggct aatgaaacag
     2341 caacaggacg caatgaagcg cagtatatgc aacgttcatg gactagcgct tatactgctt
     2401 atattgtggg agacggcgga attgtttatc aagtcggtca acctggttat gtacagtacg
     2461 gtgctggttc gtatgctaat gccaacagtc ctgtgcagat tgagttacaa cacacacatg
     2521 ataaagcaac gtttgagaaa aactacaagg catacgttga attggctaga gattcagcaa
     2581 tgaaatatgg tattccatta acgttggaca ctccttataa ccaaccggga atcaaatcgc
     2641 atttatgggt aacacaaaac atctggggcg atcatacaga tccttacggt tatctttctg
     2701 aaatgggcgt aagtaaagaa aaattagcat atgatttagc tcatggattt accgatgaaa
     2761 atccaacaac ttctgaaaac aagcctgtca ttgatccaac acgagctggt gcagctaatc
     2821 ctacactgac agatggaacg aattacgccc acattgatca gtttggagaa atcgaaaatg
     2881 caaatttgca tgtagctgga tggcacattg ctaactataa atacgagtat atcttcatta
     2941 tggattacaa tactgggaaa gaattagctc gagtaagagc tgatggaatt tatagagcag
     3001 atgtaaatca agcttataat acttctggaa atgttggcta tcatgtatct tttaacatgc
     3061 gtaacttccc taataagaaa gtctatgtca tgatgcgggc aacgaatgat ccagagggaa
     3121 acactaaagg cggtgcgcaa gatttccatg acaaacgttg gtatttaaat attcctaaac
     3181 gataaaaata gctcctcgtt gaggagcatt acataactat attgacaact ataaaaatca
     3241 ttcgataaaa tagtgatgtt atcgcatatc ttcactatca cccataatag tcacactcca
     3301 agctatgcga taacaggttt gttgccacac attctactgg ttgattgttt atggctttat
     3361 gtggcaacaa ccagtaccct tagctcagtt ggtcagagca gacggctcat aaccgtccgg
     3421 tcgtaggttc gagtcctaca gggtacatta acgtagccat ttgaatcgtt ctgtgttaga
     3481 attttttgaa gagtattata caagctaaag cttttcttca ttgccactca aatgagtggc
     3541 ttttttatgt atccttttat ggattaatga aaggatgttt cacatagtta tatttctgta
     3601 tatttgaaaa gttttacttt gatttttaaa tagaaagaca tttgggttat attgtgagat
     3661 aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag tgtgttacgc
     3721 tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga aaaaaagtga
     3781 gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga ttttatagca
     3841 ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa cttaaaaaat
     3901 atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta ggtagcctac
     3961 tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca taacgccgat
     4021 gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat acgtctttct
     4081 tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag tagagattaa
     4141 ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc ttataaaaat
     4201 ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca ttaaaaagca
     4261 taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat atcgagtttt
     4321 tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt tattttcgga
     4381 aaaagctaat atagctaaga ttgaaacatc catctggaat ttatcttttt tactgctttg
     4441 atcataaaag tctgcatatt catcactgat attttttcta ataaattctt ccatcataaa
     4501 aatcacctca aaacgattat acgaacttac gttcttgttg taaagcgata tttgaggggc
     4561 aaaaaagggg cataatttga ctgatattaa gttagctatt gatatatgaa tgacatacaa
     4621 aaatataaaa tcttttaaaa atgcttatat atcaatgttt ttgccattct tttatatatc
     4681 tgttatatgt caaaaaaatg tgttcaattt caatttgtat ttgaataaag aggttgtgaa
     4741 aaagctgtcc tgcatcaagt aataagaaca gccaatcaaa aacagcttct catgtttttg
     4801 atttggttgg acttattacc gcagatataa gcttttgagc accgtgaata aagaggttat
     4861 gaaatcaacg ctctaacctg agtattagag aaaaaaacgc aaaacatatc atttatgttt
     4921 tgcgtttttt atttatcttt ttttctcaat acaatataaa ataggcggct ggttcttttg
     4981 gttgataaac cgataattca gcacattata tgtttcctgt ggcagtttgg aacaaaaaga
     5041 atcaaccata tccagttctt tttctccacc ttcatgacca tagtagacga caaggatcaa
     5101 tcgtccgcga ggcacaagtc gtttcaatat ttcttccata gctgtgcgtg tagtctctgg
     5161 catagtgatg attgctttat cactttttgg gagataccct agattgaaaa tccctgcttt
     5221 gagattttgt tgttcagcta tcacttctcc taacgtttcg tggccttgta gaaacaatga
     5281 gacacgttct tctaactgac gctgttctag cctttcacga gtagcatcga ttgcttgttg
     5341 ctggatatca aaactataga catgaccagt ttttccgatg tgttctgcta gaaaaaccgt
     5401 atcgtggcca ttgcccatcg ttgcatctac gacgtgatcc cctggttgca agatttcttg
     5461 cagtaatgta tggctgaaat gcaaagcagt ttgcaacatc agacgctcac ctttttccgt
     5521 acatcatatt ttccttggaa gctatctctg cgaagaagtt cttcatcaat tgcattcaag
     5581 acttcccatt ttttcaagct ccacattggt ccaatcaagg aatcccacgg cgcatcccct
     5641 gtcaaacgat ggatgatgat ctcttttggt atcatttcca gctggtcgca aatgacattg
     5701 acatagtcag gacggctcat taattgaagg cgaccttcat gatagtcccg caacattctc
     5761 gttttgcgca tcagatgcat caaatggagc ttgattcctt ggatatctga atctaaaatg
     5821 gtgcgtctga cattttcttt catcatttct aaactttcgc ctggtaatcc gttgatcaaa
     5881 tgcgtacaaa cacggatatt atgtttgcgt aatttagcca cgccatctaa ataagtttga
     5941 taatcatgtg ctcggttgat cagttttgaa gtttcttcaa aagttgtttg caatccaagt
     6001 tctacccata aatacatccg ctcgttcagt tcagcaaggt aatcaactac ttcatctggc
     6061 agacaatccg gtcgtgtacc gactgataat cctacgacac ctggtaaatt gactacttgc
     6121 tcaaatcgtt ctttgattat ttcaactggc gcatgggtgt ttgtaaaatt ttggaaataa
     6181 acgatgtatt gttggacatg aggccatttt tgatgcatca tgtcgatttc tttctggaat
     6241 tggattggaa gagggtcttc aggtgcaacg atcatgtctc ccgaaccaga tacactgcaa
     6301 aaagtacaac cgccatgggc aacagtccca tctcgattcg gacagtcaaa tcctccatcg
     6361 atcggcacct tgaaaatttt cccgccaaac tcatttcgta aagcgtaatt ccaagaatga
     6421 tatcttttat ttccttctga ataaggaaaa gtcattaaaa gtccttcttt ctgtaagagt
     6481 tatctatttg ttttcttttt tctctataca ctggataagt caggcacagc caagaaagac
     6541 tgagtaagta accgccgatg atatcagtag gaaaatgaac gcccagataa atgcggctta
     6601 tcccgacact tactataata aacatcatca gcaattgaag caatgtgcgt aaggttcttt
     6661 tttgaacaaa agctggaagc agaaatagta aagaaccgta aagaatcata ctgcctgctg
     6721 catgtccact agggaaactg taacttgttt caactactaa gtgcgccaat gaaggacgct
     6781 ctcgcatgaa aaatagtttg atcaccgggt tgatgattcc agcaattaca ataaatccag
     6841 aaacgaacca aaatgtttct gccctttttt tcttatacca catccataaa gccgcacaag
     6901 caaataaaat gatgacggta agaggattac cgaatttagt cataaagagt tggtagctgt
     6961 tcagatcagg ataagaagct ctgacaatgc ctgtaaacca ctgatcgaaa ccagaaagcc
     7021 acgacagatg gaactttacg atccagccca aaatgaaaaa caggacaaga aagaagctac
     7081 cagccacctg aaatgatgtt ttatatttca tacaattcct cctttgaagc aatgacttat
     7141 tatagcattt ttggtccagc gaaaaaaacg gtaaagagat aaaaccaatg tatgattcct
     7201 ctttgaataa ccaatattct tataaaaaaa ataactgtaa tcggaaagac attttttggt
     7261 aatgcaaatg aaatgttagg gaaataaaat cagtgttttt cgaaatcctt ttaaacaaga
     7321 acgtagacgt aggaagaact tgaaaatgaa cagaagttgt tcattacggt aaattacaat
     7381 atgattacaa aaaacaacaa agctcttaaa taacttttaa taaatattaa gagaagttat
     7441 aattattaat aatagataaa ttgaagttgg aggatagaag atggaaaaca ttgcaagaaa
     7501 agaacggaga cgtttagaac agaccaaacg ttttcgaaaa gcaaaacgag gggcagcaat
     7561 cgtgggaact gcgatggtag gctgttctgt cgctgcccca ttagtacagc cggttcctgt
     7621 tcaagcggat gaaacacctg ttcagttcgg cgcaagaatc aatacagcgg catttatcac
     7681 agaaattgct acttatgctc aaccaatcgc tcaagccaac gatctatatg catcagtgat
     7741 gatcgcacaa gcagttgtag aaagcggttg gggcagtagt gcgttatctc aagcacctta
     7801 ttataatctt tttgggataa aaggcagcta tcaaggacaa accgtctaca tggatacgct
     7861 tgaattttta aataatcaat gggtaaataa aaaggagcct ttccgacaat atccttcttt
     7921 tgctgaatct ttcaatgaca atgcctatgt acttagaaat acttcatttg gcaacggtta
     7981 ttactatgcc ggtacttgga agagcaacac gaaatcctat acagatgcga cagcttgttt
     8041 gacagggcgt tatgcgactg atcctggtta cgcaggcaaa ttaaataata tcatcgcaac
     8101 ttacggattg acccaatatg atacaccagc tagcggaaac gcaggcggtg gagcgactac
     8161 aggtggcaat aatactggaa gtgcaggtag tactggcggt tcaagcaatt ctggtaatac
     8221 aaactcaggt tcagcaactt cgaccactta tactgtccaa tctggtgatt ctgtttgggg
     8281 aatctcaaat aaattcggta tcacaatgga tcaattgatc caatggaaca acatccaaaa
     8341 caatttcatt tatccaggac aaaaactgac aatcaaaggt agccaggaaa atggctcttc
     8401 gacgaataat tccggtaaca ataccaattc ttcaggaaat gctggtacat caaacggagg
     8461 acaaacaact ggtgcgaaat acactgttca atctggtgat tctgtttgga agatctcaaa
     8521 tgatcacgga attacgatgg ctcaattgat tgaatggaac aatatcaaga acaatttcgt
     8581 ttatccagga caacagctaa tcgtatcaaa tggcggttct tctaatgctg gagcaaatac
     8641 tggctcaaat actgggaata caagtggctc tagttcgtcc agctcaagtt cttctaatac
     8701 agcttcagga acgaaatata cagttaaagc cggagactct gtttggagcg tctcaaataa
     8761 atacggaatt acgatggctc agctaatcca atggaacaat atccaaaata actttatcta
     8821 cccaggacaa caactgactg tttcaaacag tggatcaaca aacagcacgt caggtaatac
     8881 aggttcgtca accaatacaa ataacagcag ttcagttaat actggcagca gttcgtctca
     8941 aggcacttat accgtgaaag caggtgaatc tgtttggagc gtggcaaata aaaacggcat
     9001 cacgatggat cagttaatcg aatggaataa tatcaaaaac aacttcattt atccaggaca
     9061 gcaattgatc gttaaaggtg gaacttcggc aaatacaaat acaggttcaa ctacttctgc
     9121 agcgaaacca aatacaccga atacgtctgc tacgacaagt acatcatcca ctggtaatac
     9181 gatgtatacc gtgaaagcgg gcgagtcagt ttggagcgta gcgaacaaac atcatatcac
     9241 gatggatcag ttaatcgaat ggaataatat caaaaataat ttcatctatc caggacagca
     9301 agtgattgtg aagaaaggtt catctcaatc aacaaaccaa caatcagtta ctggttcgaa
     9361 aacctatact gtgaaatctg gagaatctgt ttggagtgtt gctgacagtc atggaattac
     9421 aatggctcaa ctaatcgaat ggaacaatat taagaacaac tttatttatc caggtcaaac
     9481 gttgattgta gctaaataaa atagctgttc gaagaaataa cgattgaatt ccacaaaaat
     9541 atttgttact ataaggctac ttaaatatga caacacttga aaaggaagta gtaaaaagac
     9601 tgaagtttca gagagcgcat ggctggtgag aatgtgcacg gatgcttttg aactcgcctt
     9661 tgagttgccc ttgctgaata cccgagtaag caagcggcgg tgacggccgt tacaacgttc
     9721 gaggcctttt cactaaggta aaattaggtg gtaccgcgta gattacgtcc tataaaaaga
     9781 gcaatctttt tataggattt ttttatttct tttgtgaagt ttgagaaaac aaagcaaaag
     9841 gaaataaaaa taaacagatt ctgccggttt tttcgttata tttataatag aaacaagtgt
     9901 tttcaataga acaggaggac agcaaatgag ttacaatcat aaagaaattg agaaaaaatg
     9961 gcaaaaatac tgggcaaaaa cgaatacttt caacacccat gatgatcctg aaaagccaaa
    10021 attttacgca ctggatatgt ttccctatcc atccggtcaa gggctacatg tagggcatcc
    10081 tgaaggttat acggcaacgg atatcctttc acgtgtgaaa agaagtcaag gatacaacgt
    10141 gctgcatcca atggggtggg atgcattcgg tctgcctgct gaacaatatg cgctagatac
    10201 aggaaacgat ccagctgagt ttacccaaaa aaatatcgaa acattccgac gccaaatcaa
    10261 ttctttagga tttagttatg actggaatcg cgaagtgaat acaactgatc cggagtatta
    10321 taaatggaca caatggatct ttacgaagct ttatgaaaaa ggtctagctt atgaagcaga
    10381 agtagcggta aactgggtac ctgaattagg aacagttatt tcaaatgaag aagtgatcga
    10441 tgggaaaagt gaacgtggtg gctatgatgt agtcagaaaa ccaatgcgcc aatggatgct
    10501 gaaaatcact gcttatgctg atcgcttatt ggatgatcta gaattagttg attggccaga
    10561 aagcattaaa gaaatgcaaa gaaattggat cggtcgttca gtaggagcaa atattgaatt
    10621 taaagtggct ggtacggata aatcctatac cgtatttacg acacgaccag atactctatt
    10681 cggtgcaact tactcggttc ttgcacctga actagatctt gttagagaaa ttacgacccc
    10741 tgaacaaaaa gctgctgtag aagcttatat cgaagagaca gctaaaaaat cagatctaaa
    10801 gagaacggat cttgcaaaag aaaaaacagg agtatttact ggtgcttatg caatcaatcc
    10861 agttaatggc aaggaaatcc caatctggat cgccgattat gtattatctt catacggcac
    10921 aggagccatc atggctgttc cagcacatga tgaacgtgac tttgaattcg ctcaaacttt
    10981 cggactagaa attcttccag ttatcgaagg tggagatgtt gaaaaagctg cttacacaga
    11041 agatggtacg cacatcaact ctgaatttct agacggcatg aacaaacaag aagcaatcga
    11101 taaaatgaat gaatggctag aagaaaatgg cgtagggaaa aaagaagtca gctatcgctt
    11161 acgtgactgg ctattctccc gtcaacgtta ctggggtgaa ccaatcccaa tcatccattg
    11221 ggaggacgga acagtcacag ctgtcccaga agaagagcta ccattacggt tgcctaagac
    11281 aaaaaacatc aagccaagcg gtactggaga atctccatta gcaaatatcg aggagtgggt
    11341 caatgtcgtt gatcctgtaa caggtaaaaa aggccgtcgc gaaacgaata caatgccaca
    11401 atgggcagga agctcatggt attacctgcg ttatatcgac cctcataaca aaaaagaact
    11461 agcagattat gaaaagttga agcgttggct accagttgac atctacattg gtggagcaga
    11521 acatgcggta cttcatttac tatatgcacg attctggcat aaattcttat acgatatcgg
    11581 tgttgttcct acaaaagaac cattccaaaa actatacaat caaggaatga tcttaggcga
    11641 aaacaacgaa aaaatgtcaa aatctcgtgg taatgtagtc aatcctgatg atgtggtcga
    11701 aacttacggt gcagatacac tgcgcatgta cgaaatgttc atggggccat tagatgcttc
    11761 gattgcttgg agcgaaaatg gcttagaagg aagccgtaaa ttcttggatc gtgtatggcg
    11821 tttgatcgtt gatgagaaca ataaaatgcg tgaccgtatc actacattga atgatggcaa
    11881 gcttgataaa gtgtatcatc aaacagtgaa aaaagtgaca gaagactatg aaaatcttca
    11941 tttcaataca gcaatctccc aattgatggt ctttatcaat gaagcctata aagtggacgc
    12001 tctgccttat gaatatatcg aaggatttgt tcaactactc gcaccgattg ctcctcacat
    12061 gggtgaagaa ctatgggcaa tccttggaaa tgatggtggt atttcttacg caccatggcc
    12121 aacatacgat gaagcagcac ttgttgaaga cgaagtagaa gttgttttcc aagtaaacgg
    12181 taaagttcgt gcgaaaagca atgttccccg agacttagga aaagatgagc tagaaaaagc
    12241 agcattagcc aatgaaatag tgcaagaata tattgaaggc aaaacagtca gaaaagttat
    12301 tgcggtacca aacaagttag tcaatatcgt tgctaattaa ttggctcttt gtcaataagg
    12361 actgatgagt tgtgcaaaat caaatctggg ccagaatgaa cttcattctg tctcagattt
    12421 tttgttattt caaattgatt aattgattga tcaaaaaggt tttgattttc atgttctcaa
    12481 acacgttcaa tgtctttttc ttctgaatgg tcttgtaaga gatctctgta atcatcaaat
    12541 ttttgtctat taatcttagt attttttgat gtataaacta cctgaaattg agagtacata
    12601 ccaaattcat aaatttttta ttagtgggac tagtgattgt atggattttg acgcataatg
    12661 aatgaagtat gagaaaagga gagacaaatg gatcaagaac tatttattat cgaagtatta
    12721 tcagctaaac aaaaaattat cgagaaagcg aaaaaaagag cgaaaagaat aatgttcaag
    12781 cttttaagct tagtattttt ctctggtttg ctttttttcg gagctgtatc gttcctatat
    12841 cgccatatga gtgcagtagt ccgttcagct gaagggatgc aaaaattaaa taatttgtat
    12901 cacttttcgt ggggagttgg cggaataaca attgttgtgc ttattgtttt tcttctactt
    12961 atttggctcc gttaccatca attgtcgcgt catttcgatc aatggcttag tcaaagagca
    13021 caacagtttt tacataataa accaacttct caagatgatg actatcttta ttttgagaag
    13081 acttcgacga gtcaagcttt tcggatacaa aaaagtgatt gccagttatt gctgagatca
    13141 cttgatgatt tacctatcta cttaggcgaa agcaaatcaa aattcggaat gaacacattc
    13201 catatttttt tactagaaaa cgaacccaga tgggaaaaaa gccagtcttc gcctggtgga
    13261 tacctgacaa ctaagatgag tacattagtc actggttttt tcctcattct tttagctgga
    13321 acaatctatt atgggagata cactcagcca aaaatgtctt ctcatataaa tgctgcttct
    13381 cacatgacaa tgaactcaac taacgatacg catatacaaa agacaaatga agggttgtta
    13441 gctgatccta agcaaaaggt aaatttgaca acacaaaaga taaatgatct caaacttgat
    13501 acagagacgc actctctcta tatgacaaca gatagtggtg ctaattgggt atttgtgccg
    13561 attgattcgc aatggttgcg ttttggcgaa tacaccctga caacggggct gattcctgaa
    13621 ggttattgga tggacaacac atatgatatt tccgccgatt tctcatggtt tatctattct
    13681 tctggggatg atgtacgtct gctgacttca agagataatg gtaaaacatg gcaaacgtct
    13741 attatcaccc aatcagttca gccgatacgt tatcgaaaag cggtatttta tggcaacgat
    13801 ggtctattta tctgtacatc agccagtggg atgtctgcag aaactttgta catttacact
    13861 actatggatg gcggaatcac ttggataaaa agtggaagta caatgatcaa tcagccagta
    13921 caaaatgtca gcttcgttac aacttcttta ggttttgttt cgacacgtga aagtttgttt
    13981 tatacaaaca acggaggacg ctcattcaaa gaatcagttc tgtcgattcc ggaaaactat
    14041 cagacagggg ggatagatct ttttcaatca ccaaatgaag ttactcaaat aagcgcgaat
    14101 acgttagaga caaaatttta tttgttgaaa aaaggaccaa tcgatgcagg gaaaatgttt
    14161 gcctgcctct tccgttcaac tgataacggg gaaacatggc aattcgttca gcaactatcg
    14221 caagtagaac aaggagatta aagaagagag attgtgagcc tgacgttcag caccgagtca
    14281 caagaaacaa attcaaaaaa cagcttctca tatttgttga atagtgtaac tcgtgattcg
    14341 gagttgacaa gagcaacaag aagcgatttt gaagtatagc ttctcgtatt ttacaagatc
    14401 gtgtcttatt gccgataggt tgtctcttga acaccgttta ttcgtaaaaa gggattgtga
    14461 caagtctttt gtcactactt accgaacgaa actgaaggag ctatccaaaa agtatcatta
    14521 gtattattct ttgtgtttct agtggcacca ctagtacatt taaaaaagct atcgatcatg
    14581 gactaatcat acatgatcga tagcttttta gataatgttt gttacgctat ctgaaattga
    14641 ctattataaa gactggcata aagcgttggt gattggagta gttcagaatg tgttcctttt
    14701 tcgacgatat cgccattctt cattacaagg atcatgtctg cattttcaat cgttgacaaa
    14761 cggtgtgcaa tcacaaaact tgtcctatct tttgtcacag cttccattgc ttgctgaatc
    14821 aatgcttctg ttcgtgtatc tacgctagaa gttgcttcat ctaaaataac gacaggagga
    14881 ttcgctagaa tgactcgagc aattgtcaat aattgttgct gtccttgaga taacgtgccg
    14941 ttttcgctag aaatgatcgt atcatatccg tctggcagtg ttcgaataaa gtgatcacat
    15001 tgtgcgattt cagccgcttc gataatttgt tctcttgtgg catccatttt cccgtaggcg
    15061 atattatcag ctaccgttcc ttcaaacagc caggtatttt gtaaaaccat cccaaaagaa
    15121 cgacgtagtt ctccacgtga aagcttggta atatcaagac cgtcgaattc gatctttcct
    15181 ttattcagtt cataaaaacg catcagcaga ttgactaaag tcgtttttcc tgcaccagtt
    15241 ggaccaacga tcgctaccgt ttgttttggc tttactgaaa aatcgacttg attcatcaat
    15301 attttatctg atgtgtaacc aaactgtaca tttttgaaag ctatttttcc tttcggttgt
    15361 tctaaaagtc ttgcatctgg aaggtctgga atttcatctg tttcatctaa tatctcaaat
    15421 atcctatcga ttgctgccat tgcggattgg atcgagttga tcacataaga agcggtagaa
    15481 attggttcag aaatctgatt gatgtactga agataagctt gtaagaagcc gatcgtcaca
    15541 ccacctgata gaacaagcat agcaccgaat accgcactta tgatgaaggc gagttgatta
    15601 atgaaacgaa tggccggata gatagcacag ttcataaatt gagcacgttt aaaagcgttg
    15661 tattgctttt tatttacttc gtgtatagca gcttctgcat gagcttgctg attgaatgtt
    15721 ttgaccacta gattaccagc taagatttct tccattcgat tatttaaaac acccagttca
    15781 tactgattct gttcagaaat ctgtttgttt ttgttcgcaa tcttagttgt aacaattgaa
    15841 ctgccaacga tcagcaataa gacgagcaaa gtaagcttgc tttcgatata aaacagcatc
    15901 agaccagcag cagctattgt tacgacagaa gtaaaaaatt gattgatacc tgtcaataac
    15961 acttgagaaa gttgattgag accggtagtt gtccgactaa tgatgtcacc tacttgatga
    16021 ttgtcaaaaa acgacatcgg taatgttttg aatttttttg tgatttcttt tcgcaccctc
    16081 aatgtgatct tttcgcttaa cgctgccatt gtccgttcct gaataaatga agtgatgctg
    16141 cttaagactg aacagccgat cagcaataag acagggaaca acaatgcttg tttgatatca
    16201 ctgaaagaaa gctgtccaat acctgttttt cttatttctg ataagagatc atcgatcccg
    16261 attcccatga tcaagggcat ggctacgact aataggttcc cgaataaact gcatagaaac
    16321 aatccgtaaa aatatttatg ttcaggtttg atcattttcc aaaaacgtat caaagcacgc
    16381 gtgttcaacg tttttttctt ttccatcgta tcccttcttt ctatattcct tgtgattttg
    16441 caaactcctg ataagtttgg caattagaca gtagttcttc atgagtacct tgtccaacga
    16501 tttttccttc atctaaaaca atgatgttat ctgcttgttg gattgtactg aggcgttgag
    16561 caacaatcac gagagttttc tccgacatgt gtttatgaag ggctgctctt aatgctgcat
    16621 ctgttttata gtctaaagcg gagaaactat catcgaatac atagatctct gctggtttga
    16681 tcaacgctcg agcaatacat atccgctgtt tctggccccc tgaataatta gaaccgcctt
    16741 gagcaacaaa gctgtccaat ccgtcaggaa gggtgtccac tagttcttct aattgagcga
    16801 tcttgatcgc tttagacaac tcttctgttg aggcatattc atttcccatc tgcaagttgc
    16861 ttaaaattgt cccgctgaat aagaaagcct tctgagggac ataactgatg tgggagcgga
    16921 tctgttcttg agtcatttga gtaagaggaa taccaccaag ggttactttt cctatcgtag
    16981 ggtcactaag tctcaagaga agtttcgcca aactactttt tccagatccg gtcccgccaa
    17041 cgatcgctgt tgtttttcct ttaggtattg tgaaatcaat atcttctaga actggatcag
    17101 agatttcagt atatttaaat gtgacatgtt caaaagcagc tgcatttgtc tgatcaaagg
    17161 ttggttcagc tactgttttg atcgtctctt cctcaatttc gatctctgct tgcagtattt
    17221 cttctatccg tcgtaatgag gcaaacgatt tagggataat gaccaatacc atagctgcga
    17281 tcatcaagta agcaagtgtc agcatagagt attcgactac agcagtcacg acaccgattt
    17341 gtaatgctcc ttctgctaca agatttccgc caaaccacaa aatagaagca taaacgattc
    17401 ccatgagcaa aaaagcaatt ggagtgaccc aagcaaatat tcggttcact ttgatcattt
    17461 gattagcata gtcagcaaag ctcttattcg ttcgcttttc ttcatatgat tgattatcaa
    17521 aggcacggat catattaatc ccagtaaaga attctctcaa tgtgatggtg atccgatcca
    17581 tttttggctg gatactcaaa gataaaggtg cccctcgttt catcagtcga taaacaatcc
    17641 atacataaaa aacaacagag atcaatgtaa cccatgttag taatagagaa taacgaaaca
    17701 tcatgagaat ggtgaaaacg caaataaccg gtgctggtaa gatcatttgg caaaataaaa
    17761 caatcatttg ctggacatta tcgacatcat tcgtcattct tgtcaaaaga gatccagaac
    17821 taaaacgact gatatccttc attgacagct gttggaattt acggaaatat ttctcccgcg
    17881 tttggaatcc aaacctagcg gaagccaatg ctgaaaagta actagaggca actgcagcga
    17941 ttgttcctag aaacgctaca agcagcattt caactccaat tttttttata actgaggctt
    18001 cgccacttgc tatacctgtg tcgatcagct ttgctactaa ttttggtaca cctaatgtac
    18061 cggcgatttc taaacagatc atcacaaagg taaggagagc gagtcctttg tttttctgta
    18121 agaatgtttt taagatagac atattattcc tccttttttc ctgatacttt tcgattctat
    18181 accttcaagt aacttgaagg tcaatagaaa aaagaataaa ctgaaaaaga ggtgagaaaa
    18241 aatgaaaaaa atgtatacga ttggccaagt atcggaacta tttgatttgc caaaatcaac
    18301 gatccgctat tgggatgaac aaggattaat ccgttcaagt agacaagaag agaatgatta
    18361 tcgattgttt gatatcgatg atatttttat gctttatgat atcgtttttt atagaaaatt
    18421 agatataccg atcaaacaga tgaagaattt atatggaaaa acattgacgg agttatatga
    18481 gacacttgat gaaacagaaa gaagaatcca taaagaactg gtggtgatga aacaaaaaca
    18541 aaaagagata agggagcaaa aaaaacagtt gaaattgatg atcgatacaa acgaggaaga
    18601 atttcctgta gaagagatcc cttttgactg tatgatctca acggagtttg aagatattgt
    18661 ggaaatcaaa aaatttctac ctaactattc aagctttggg atgatgagta tgccagcttc
    18721 cagttcacag acgatgtatg gattttttat cgaccctagt gaagtacatt tatttactca
    18781 agacgtgatt tgggagaaaa aagatacggc tgtttatcgt cgattcttat tgaaatccga
    18841 gatgaaccat gcggaacgta ataatatttt agaaatcaga gaaagaatgt atgaaaaagg
    18901 atggaaaaca ggagaagtga tcggccagta tctattgacg aatacggatg aaaacaatat
    18961 cagaactgaa tattatcatg catggataga aatgaaaaaa taggaaatat gtgtttaaga
    19021 taactcgaaa atgatggttg ttttagttat aaagaaaaat tttctggttc gattacgggt
    19081 ttaatgtggg ggattattag tcgtggatag atagaatttt agttttttag aggaaatatg
    19141 tctttaaaaa tggaatggtt gcaattctta gcgatatata cgatgtaatt gtcctcttaa
    19201 aaaagtatgg aaaaataatt gtattgggtg ttatgtataa ttcatggtga actgagacta
    19261 atgagaagaa aaattcatat tgttatgagt gagattgtat cagatgagaa tgataaatta
    19321 aataggtgca taaaagcatt acacttaaaa tacattgttg cttacattat tacaatatgt
    19381 taataaggca aaaataaata gatggattga ataaacatat tttttaatca aatagtccct
    19441 actgtgactt t
//
LOCUS       DAYYCQ010000054        19252 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000054 DAYYCQ010000000
VERSION     DAYYCQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.054"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..1606
                     /locus_tag="ACHAYF_001863"
     CDS             179..1606
                     /locus_tag="ACHAYF_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579301.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1698..2063)
                     /locus_tag="ACHAYF_001864"
     CDS             complement(1698..2063)
                     /locus_tag="ACHAYF_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8579302.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2060..2425)
                     /locus_tag="ACHAYF_001865"
     CDS             complement(2060..2425)
                     /locus_tag="ACHAYF_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8579303.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2617..2865)
                     /locus_tag="ACHAYF_001866"
     CDS             complement(2617..2865)
                     /locus_tag="ACHAYF_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579304.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2864..2992
                     /locus_tag="ACHAYF_001867"
     CDS             2864..2992
                     /locus_tag="ACHAYF_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8579305.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3049..4743
                     /locus_tag="ACHAYF_001868"
     CDS             3049..4743
                     /locus_tag="ACHAYF_001868"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8579306.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4843..5016
                     /locus_tag="ACHAYF_001869"
     CDS             4843..5016
                     /locus_tag="ACHAYF_001869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579307.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5394..6527
                     /locus_tag="ACHAYF_001870"
     CDS             5394..6527
                     /locus_tag="ACHAYF_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8579308.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6558..6878
                     /locus_tag="ACHAYF_001871"
     CDS             6558..6878
                     /locus_tag="ACHAYF_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8579309.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6907..7371)
                     /locus_tag="ACHAYF_001872"
     CDS             complement(6907..7371)
                     /locus_tag="ACHAYF_001872"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8579310.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7503..8756)
                     /locus_tag="ACHAYF_001873"
     CDS             complement(7503..8756)
                     /locus_tag="ACHAYF_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8579311.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8785..9102)
                     /locus_tag="ACHAYF_001874"
     CDS             complement(8785..9102)
                     /locus_tag="ACHAYF_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8579312.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9128..9448)
                     /locus_tag="ACHAYF_001875"
     CDS             complement(9128..9448)
                     /locus_tag="ACHAYF_001875"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8579313.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9912..11327)
                     /locus_tag="ACHAYF_001876"
     CDS             complement(9912..11327)
                     /locus_tag="ACHAYF_001876"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8579314.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11344..12660)
                     /locus_tag="ACHAYF_001877"
     CDS             complement(11344..12660)
                     /locus_tag="ACHAYF_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8579315.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12810..13508)
                     /locus_tag="ACHAYF_001878"
     CDS             complement(12810..13508)
                     /locus_tag="ACHAYF_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8579316.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13797..14786)
                     /locus_tag="ACHAYF_001879"
     CDS             complement(13797..14786)
                     /locus_tag="ACHAYF_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8579317.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14866..15699)
                     /gene="msrA"
                     /locus_tag="ACHAYF_001880"
     CDS             complement(14866..15699)
                     /gene="msrA"
                     /locus_tag="ACHAYF_001880"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8579318.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15874..17508)
                     /locus_tag="ACHAYF_001881"
     CDS             complement(15874..17508)
                     /locus_tag="ACHAYF_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8579319.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17495..>19252)
                     /locus_tag="ACHAYF_001882"
     CDS             complement(17495..>19252)
                     /locus_tag="ACHAYF_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579320.1"
                     /translation="QNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYS
                     VNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPA
                     VVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTV
                     DIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSA
                     LIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPF
                     KTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVV
                     STWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLI
                     IGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIK
                     ERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKV
                     VNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNK
                     LKDSRYVTKKEINKKNENK"
BASE COUNT         6116 a         3760 c         3194 g         6182 t
ORIGIN      
        1 ttaaaaattc gcaatttatt ttttaataga attatctgtt ttccaaattt tataacataa
       61 aaaacctttt ttaacggcat ttatatgctt tttcagaaat taactgatga tttcaaaaag
      121 aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag gctctactat
      181 gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg ggcaattcta
      241 tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac
      301 tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg cttatacaca
      361 tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat gcaagatcat
      421 gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt tggaagaaga
      481 tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca aagcttattc
      541 attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg accgctactc
      601 ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg
      661 cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc gtttcattga
      721 atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa tcttatcgat
      781 tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag attcaaaatt
      841 tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat gggaaaacac
      901 tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac tattcaatct
      961 ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac agctacacca
     1021 agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg aatctcactt
     1081 caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc
     1141 ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa atgaggaaca
     1201 gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc agttacctta
     1261 tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga gcggaattct
     1321 ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg ttcattatct
     1381 gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg caccaacaat
     1441 ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc gggtattatg
     1501 tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc ctatttcgat
     1561 caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa
     1621 aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat ctcttgatac
     1681 acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc tttatttaaa
     1741 aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat atcctgcaag
     1801 gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa acttagaagt
     1861 atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc gctttgcact
     1921 tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac accccaaacc
     1981 aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata tggaagaaga
     2041 aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct ttgtcttcta
     2101 tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt gcaagcttca
     2161 aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg actagtactt
     2221 ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta cgagtattac
     2281 gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga acaagaacga
     2341 tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc cattctagaa
     2401 attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt tgttcctatt
     2461 cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct aatgataacg
     2521 gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca taaatcgtgg
     2581 aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca gcttttcaaa
     2641 gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac catacacctc
     2701 ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc
     2761 ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga
     2821 actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat
     2881 caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac
     2941 aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat
     3001 ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat gagaaaaagt
     3061 aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa
     3121 gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat
     3181 gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa
     3241 aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat
     3301 ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa
     3361 gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt
     3421 ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg
     3481 actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa
     3541 gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca
     3601 acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg
     3661 atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa
     3721 tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt
     3781 ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact
     3841 gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt tgtggtcgat
     3901 gaagatacag cagttatttt cactaatggg tccattacag attcagctac tttaggagat
     3961 tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa
     4021 gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca
     4081 agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc
     4141 cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc
     4201 acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca accgcatttt
     4261 acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt
     4321 ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg
     4381 attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa
     4441 attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc
     4501 aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac caatctggcc
     4561 tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta
     4621 cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa
     4681 acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa
     4741 taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac caggctaaaa
     4801 cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc ttttgaacac
     4861 cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc agccaagcta
     4921 aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca agcttttgaa
     4981 caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg
     5041 tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa ctgatccctt
     5101 ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat tttttcaacg
     5161 tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat gattaccatt
     5221 acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta tagaatgttt
     5281 catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa aatgaaatta
     5341 tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct ccaatgaaag
     5401 cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat
     5461 tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc tccgatctgc
     5521 atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga cacgaaccaa
     5581 tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg
     5641 ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac atggaaagcc
     5701 aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt ggcaagttga
     5761 acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca gactctactt
     5821 catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta tcagatattt
     5881 tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat acagttgtga
     5941 ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg gctggagcag
     6001 agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct aattacaatg
     6061 gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg tatgaactgg
     6121 ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta gagcctgtca
     6181 aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca ttacagatgg
     6241 catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat atgactccag
     6301 cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag catggacagg
     6361 caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga ctattcgatc
     6421 ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca tatgagatct
     6481 ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt cagtaaaaaa
     6541 tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc gaaataggaa
     6601 aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa aatcgttaca
     6661 cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt ggaagccttg
     6721 cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct ggtgctattt
     6781 tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac gacgaaaaaa
     6841 aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct
     6901 cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac tttcttcctt
     6961 gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat ctgtaattaa
     7021 gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta atatccgctt
     7081 ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat ttggtgtcag
     7141 atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga tggtttcttt
     7201 ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc cagtacgaat
     7261 actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat tcgcgctttg
     7321 ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca atgtttaaac
     7381 ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga
     7441 caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg ttttttgatt
     7501 aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt gacgaacggt
     7561 agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc tgcaatactt
     7621 tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat aacggtagct
     7681 gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac cattggtgtc
     7741 aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa catcgttggt
     7801 tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat atatggtttg
     7861 tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc tgttcctcct
     7921 aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct atgtccagct
     7981 gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat caaaggatcg
     8041 accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa tgcaagcagt
     8101 aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac tgtatagaaa
     8161 gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa agcaaacgca
     8221 gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca
     8281 ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa gatttctgtt
     8341 gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc taatgctccg
     8401 cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc ttttcggtag
     8461 ccgttagcta aatgataagc aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta
     8521 ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat tgagttcatc
     8581 aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc tcctaagatc
     8641 attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca
     8701 atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt atccataaat
     8761 cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat caatgatctc
     8821 ttttgctaaa tctgtaaaag ctatagatgt catcagatga tcttgactgt ggaccgtcaa
     8881 aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg
     8941 atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta aagtgaaatc
     9001 accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc cgccaaacat
     9061 gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta tcctccgata
     9121 atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca ccattcatca
     9181 tgccatagtc agccatattg attacatcta aaggaatgcc tttaggtgct agcttttcag
     9241 caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct acatttttag
     9301 attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct tccattcctt
     9361 ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt ccagctgaac
     9421 atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata cttacacttg
     9481 tatataagca agtagcgtgc caactttaaa agcgctttaa aaagttgata tcatcacatt
     9541 taaggtctat accaagttgc atacacacta aaaaagaaag cgcacactaa acacacaaat
     9601 taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt
     9661 cacaactttt ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat aagtccaacc
     9721 aagttaaaaa cataagatgt tgtttttact ttgctattct tattacttga tgcaggacag
     9781 ctttttcaca acctctcaat aaacggtgtt caatcagctg accctgggta ttagctcaaa
     9841 acgctggatt cacaacctct tttatttcaa ataaccagta aatacaatga acatctacaa
     9901 aaaacttcta tttatttata gatttcgtca ggcaaatcca attctctctt atcaatcaaa
     9961 gtctgatacc acgaagcaga ttttttcaat gttctgcttc gttcatcgtt caattcgatt
    10021 cgaactaatc catatcgatt cttaaatgca ttcattggtg acacacagtc agtgaatgcc
    10081 cataacatat agccttcgca atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa
    10141 tgctgtccga taaattcgat acggtaatca tcttgcacga ttcctttctc atccatatac
    10201 tgttcttcat attctcttcc cataccgttt tctgttatca gccaagggat attatgataa
    10261 ttttctttta gatacatggc tatatcatac atgattttcg gatagatttc ccatcccctg
    10321 gaattattca ttttcttccc aggtaaatca aagacttcat aatatcgttc aggatggaaa
    10381 ggtgtctctt cattccattg ataacgaggg gcgcgaacgc gtttaggata atattgattg
    10441 attccaagga aatctactgt atgatgctga atcgtttcta aatcctctgt tgcaggatcg
    10501 aaataaatcg attgtttttg acacaactcc aggaactctt ctgtgtagta tccttttacc
    10561 atcggatcaa aaaatactcg attataaaac aaatcataca tataagcagc tttttggtct
    10621 tctggagaat tggatcgagc atagaccatt tcaggattta aaatacagcc aatccttcca
    10681 ccataatttc cgctgcggta aatctctact gctttagccg ttgctaaaac tttatgataa
    10741 ttccacatca tccacttttt tgtgttttgt tcatgcggcc agcgaacagc atccaaatag
    10801 caacgcgttt ggggaacgat tggttcgttg aatgtaaacc agttttttac cctatcaccg
    10861 taacgatcaa aggcaatttg agcatactta gcataaaaat caactacttt tcttgaagac
    10921 cagccatcat atttttccaa aagaaaagct gggacttcat aatgttccaa acaaatcatc
    10981 ggttctacgt ctgcttcgat caaagagtcg atcatttcac ttatatgctg agcatattct
    11041 tcatctacta tacagttctc ataatcagta aaaaaacgag cccaattgat agaagtccga
    11101 taatgtgtca gatggatgga tttcatcaga tccacatcct gctggtattg ttccataaaa
    11161 ttagttgcta cggctgggcc atacccatta tgccaaacaa aatgttcatt tttataccag
    11221 ctatctataa acgaatcctg tccttctttt tttcctttcc aaccttccgt ttgccaagca
    11281 gaagatgcag caccaagaat aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt
    11341 tttttagatc ccatcatttt ccacacgatt tgctactttt acaaatggca gatagatcaa
    11401 aatagaaaca ataatacaaa caagttgcgt aataactgca ccgatatttc ctgctgttgc
    11461 cagataagca ttgacgatcg gcggcattgg ccaagggacc attacaactg ctttcgccgc
    11521 aaatcctaaa gcggttgcaa aataaccaat cgttccagtg ataagaggcg ttaagataaa
    11581 cggaatagct aaaattggat tcagcatgat cggcatacca aaaataactg gttcattgat
    11641 gttgaagatc ccaggagcaa gggataactt ggtgatttgt ttcatatctt cccgttttcc
    11701 aactaacaat actgcaatca gtaaaccgat cgtgactcca gacccgccca tactcatata
    11761 catatcccaa aatggcatag taataatgtt cggtacttct tttccagctt caaaagcttc
    11821 cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt atcggcttca ccatttgatt
    11881 tccatggata ccaatcaccc aaaagacttg cgcaataaac atcaatagca aaatacccgg
    11941 taacccttgt acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag
    12001 gtacattcct gtaagttctt tgatgataaa tcccgcagcc gcgatacccg atacagtcag
    12061 aatcgtcggg aataaagcag aaaaactagc agaaacattc gttggcacgg tatcaggcat
    12121 tctcagctgt agtttcttct gtttcctcaa ccagcaataa agttcaatag aaaggatgga
    12181 gaccaacatt cccaaaaata accctttcgt atctgtatat tgtttagcca atacgttttc
    12241 tactataaca gtctgatttt cattcactaa ttcataaaca gttggattaa cagataaata
    12301 agacatcact gcgatgatac cagggaatac accatccact ttattcagct tggctaattc
    12361 gatccctatc agaaaaacag catagatggt caagaaactt agtgtgacat agctgatggc
    12421 agatgcgata ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt
    12481 ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg
    12541 aatcaatgca gcaaatgcgt tttttatcac catgatatac cttaaattat tgattctcgt
    12601 tgccacgttc cctaaaacat tggctaattt atccataaaa ctcattccag atcccctcat
    12661 tcctgcttct tctccttgac gtcatctctt tttgtctatt agtcagcgct tacatttcga
    12721 tgaacagaaa ctatttactc ctctttttta ccaaatataa aacaaaaatt catctgatgt
    12781 atttatcata aaatacatca gatgaactgt caattagttt ctttttctcc tattattttc
    12841 tgtaattctc tccgatttat tgccatatgg acagtcattg catcaaacgc agcaagtgca
    12901 ttccgccttt cgatggcctg aacgatctct gtatgaatat cgatcgtctc gattttcaga
    12961 tgctggatat cataattttt attaaacaaa gcaattgatt cattaatgat tgggataata
    13021 tgcagcaagg ctacgtttcc gctagcttct gcaatcatcg tatgaaaatc gatgtctaat
    13081 tgaacatgtt cttgtgtgcc ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc
    13141 atttggacga tttgattctt cgttgcatat tccgctgcca aacctgccat cttaggctcc
    13201 aataaatatc tcagttcaaa taagtctcgt atcatccgat cctgatcttc aatcaaagaa
    13261 aaaccgagtg ggtcattggc aatccctcgc ttatcactga caaatgtccc cgctccttgc
    13321 cgaacttcaa ggatattcct ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc
    13381 cccacctcta aataatcgga caattcatat tcattcggca gtttatctcc tacttgcaac
    13441 tgcatttcct ttaaatatct aaggatcttg tcagtagtta cttctactag ggttttattt
    13501 tcttccaaac cacatacctc cttttcatct gatgtatttt ttcgtaatta ttataacacg
    13561 tttgaatcat ctgatgattt tttgtataca aaattgatta atccgttcat agacatacaa
    13621 acgaagaaga tatttaccat aaaaaagact aggataaaat tcactcctag ccttcgatca
    13681 tttttatttt gaacgacaaa aaatatgatg ttcaataaca gacgatcttc attcgattct
    13741 tgatgtcatc ttttgttttt tttaatatat ggataaaaag tttgattaca tcgtttctat
    13801 tctagaattt ggtcgattct caattcttct tctggtgaga aatctaaatt atccatcatt
    13861 cgaacacttt cttgtaactg acttaaccga cttgctccga ccagtacact tgtaacagat
    13921 ttttgtcgca aattccatgc aagggccatt tgcgctaatg attgtcctcg tgatacggcg
    13981 attgtttgca gtgctttgat tttttccaga gttgatccaa cttgttcttc tgaaaggaat
    14041 ggtatttcct tacgatgtgc tctagaatct tcaggaatac catgtagata acgatttgtc
    14101 agcaaccctt gagcaagcgg actgaacgtg attgccccta gcccttcttc ttctaatact
    14161 ggagatagat cgtcttctag tagtgcacga gaaaacatat tgtagcgcat ctgatgaata
    14221 atgaacggtg cttcctttct ttttaaaatt tcagtcattt tttttgtatc ttctccatta
    14281 tagttggata taccaatata taatgctttt ccttgtctta caagttgcat caaagcttct
    14341 gctgtttctt ccaaaggtgt atcagggtct gggcgatgat gatagaagat gtcaacataa
    14401 tctagcccca ttctttggag actttggtca caacttgaaa tgatggattt acgagagccc
    14461 cattccccat atggcccttc ccacatatga taacctgctt tgctagaaat aatcagttca
    14521 tcacggtagg cttgaaaatc ttctctaaaa atacgcccaa agtttttctc tgcagctcca
    14581 gcagggcctc cataattatt cgcaaggtcg aaatgggtaa ttcccatgtc gaatgcgcct
    14641 cttaaaatct ctctttgatt atggatagga tcataatcac caaaattttg ccataatccg
    14701 agagataaaa tcggtaattt caaacctgaa tgaccggatt tacgatattc cattttttca
    14761 taacgtgcac tatctgcaat atacaatttt tctcctcctc atcaaatctt tttaaaacgc
    14821 tttctttttt ctatttttag tttactacat tttaaagaaa aggagttaat caaaatgatt
    14881 ttttcgtata tttttttgcc atttgttttt tactcgttga tagatcagaa attgattacg
    14941 tgcttgctgg atttttttat aacgtttcgg ttgttttttg taaaattgct gatgatagtt
    15001 ttctgctggc caaaacgtac cggctggttc gatttttgtg acgattggtt ggtggaaagt
    15061 tcctgaactg tctagtttct gttttgattg ttccgctaat tctttttgtc gttcgacttg
    15121 ataaaaaatc actggacgat attgcttccc tctatcttga aattgtccaa atgtatctgt
    15181 gggatcagta atttgccaat agattgtcag caactcgcta taacttattt ctcttgtgtc
    15241 ataaatgatc tccacagctt ccacatgacc tgtgtaacca ccacttactt gatcataatt
    15301 tggtttttca acatgtccgc ccgtgtatcc tgacaaaaca gataggatcc cttttttcga
    15361 ttcaaacggc tcgaccatac accagaaaca tccccctgca aatactgcac gttcttgata
    15421 gtcagcatct tgcatggcat gcttggaaaa gtcatatttt tgctccttat caaatccttc
    15481 cgtaatcaat aaataaaagt ccattacatc aggcgtcaga ttcattcgga tagctaatgg
    15541 acgtagtgtt gcttctagtt ttgataactg ttctttaacg gatatatttt ctcttatgtc
    15601 attctttgtc tgaatcaata cttttcgttc ccaatctcgt gtattcggat tcaaaatcag
    15661 attataaagt gtttgaatga atatttgttc ctcatccata gattctcctc ctttccgaag
    15721 aagacaagct agtgacttta gctgtaattt atcataaagt gaggaaataa gcgattactt
    15781 catcccccta cagatttttc gtataaaaaa gagagaatcc ttatcgacag attctccttt
    15841 ttaatcaatt tattttataa atgaaaagtt aatttacctt ttatggaaaa ctattttaaa
    15901 aaataaacat aaatatgcag ttaatgtaac aagataagaa ataatagtcg ttactgtata
    15961 acttgtgctt actttaatat cagcaactcc tttgactccg cttatttgta ccaatccatt
    16021 agcattcacc tgctccttta caggttgatg atttatttct acattcgttc ctaaataagc
    16081 atatacagga agtgtgatta cttggctctt ctcattagtt actctatata tgatagaact
    16141 accagttgtt ttataaggaa ggtttaccca ttgactgcca atctttgctt cgtgttttat
    16201 tatatgctcc ttattctcca atgaagcgat aggagaataa tcatataaaa aataatccat
    16261 tcctttagtg gtatacgcct ctactgtttc tttgtttaca acaatatgtc ccggctgaga
    16321 aattattttt tgattgaaat tttttttact agaaacagtt aaagtgatta ataacaaaac
    16381 agtcataaat aaataaaata gttctcttgt cttattgtat cttctatttt tgaaaaatat
    16441 catcgttaat atcagagaag agaatattat ttgcaagctt aagatccgcc aaggaaattg
    16501 taacaatttg gctggcgtat tttgcagaag ataccatggc accaaactag ttgtagctag
    16561 ccaagtcaaa cacgtaccat aataaatctt tttttctatg tttgttaatt ttttaaataa
    16621 aatcagggga atacataaag ataacagacc aactagtccc attgagtaag tagttaagtc
    16681 gttgttcata gcttcagtca gactatcgaa cacattagat gcatgagcat ataaatttgt
    16741 cacgctaggt ttgttgattt tttggtagag catttgttca aacatcggat accaaaaagc
    16801 actcgtcaga accaatgtca ttcctgccgc actaaataat tttttaaata tagcaataaa
    16861 atctgcattc ttgaaacctt tagaaaccaa acgaataaaa ataaaaatga taattaatgc
    16921 tgtactcatt aatgttgata gtacatggct gtacactagc aaactcatac tcaatgctag
    16981 ctttacagac ggatactttt cttcttttcc agagatgatt tgataagcat aaaataatac
    17041 agggataaca agtgttattg cgatagcttc ggcaattgcg gaacgatagt agatgtctac
    17101 tgtccgataa gcagagaaaa tatagagaca actaaataaa aatgccgctt ttttttgttt
    17161 taaaaaccgc tcgccataat gataagctac gagtgttgta cacaagttca ataaccaaac
    17221 gtaaataata tagcttacaa tcaaattttt tgtcatccaa tataacatta cagctggaaa
    17281 aaaagtcaga aagggataaa aataatttac accatatcca gtataattga atgtctcata
    17341 attaatcgga ctggtaaaaa tagtcgataa ccccttgatt cgagataagt gaaaagaaag
    17401 atctccagtg gtataaatac tctgtgtatt tttgaaaaag atgaaagaat atatactact
    17461 aactataatt aatccactca atattactac ttttttattt attttcattc ttcttgttta
    17521 tctccttttt agtcacatat cgagaatctt tcaacttatt aggtgcaaat cgtttaaaac
    17581 ttgaatacac cactaaacaa taagtcagaa tactgatcca taaagccatg agagcagttg
    17641 gagtatattt tgttgtaatt tctatcttat tttctccagc atttacatcc acattcacta
    17701 agccatgttt tttataagag tcatttacga ctttaccgtt tacttttacc tgtgtaccta
    17761 aataagcata tactggtact gtcacttttt gctttttgtt gcttgccact gtgtaagtaa
    17821 tgttattatc tgcagcttta tacttccctt tttcccacga ttctcctact tttacttcat
    17881 gtttttctaa gtgaggcctt tcttttatcg cttctgttgg ggcgtaatcg tataatcctc
    17941 ccatatctga tgttgtataa cgtgggagtg tttcttcatt gataacaata tgaccgtgtt
    18001 ttgattggat aacttttgcg taattttctt ttgctgctat agacgtgacg agtagaaaca
    18061 ccacagtagc gccaatgatt aaccatgaat attttttatt catagtcttt ttcataaaaa
    18121 caagcgttat gatcattgaa gaaaaaaaga tttggatacc taagatacgc caaggaaact
    18181 ggagtaagct tatcggtgtt ttttgaaaca aaaaccatgg aaataaatta gttacgcata
    18241 cccatgtaga aactacgcca acatagatga ttttttcttt tttctccatt tctttcagta
    18301 tgaataaagg aaaaatcaac gcaaccaatc ctaataaccc catagtgtat gttgtaatat
    18361 cgttatttaa cgcttgaatg atactatctg aaatagaaag tgcttctagc tgcaaatctg
    18421 ttttgaaggg actgtgtatt tcttgataat tcatctgctg caacatagga taccaaaaat
    18481 agctagttaa tgcacaagtc aaaaaaacag cctttactaa attcacgacc acaaatttta
    18541 tatcgttttt tatttgcttg cttaaaaaca aacgaactat aatcatacaa gcaatcaatg
    18601 cactacacaa tagtgtagtt aagacatgag tatacacaag tagacttata ctgacagata
    18661 aagcgatgac ttttttttct ctattgaata tcaatccata agcataatat aaaacagctg
    18721 ggataatcgt aagagcgatt gcttctgcaa ttgcggatcg ataatagata tcaactgttc
    18781 gataagttgc aaaagtatat aaacaactaa ataaaaaagc agcatctatc tttttaaaaa
    18841 acttttctcc ataataaaaa ctaatcagaa tcgtacaaat atttaataac caaatatata
    18901 gaacataact taatattaag ttatttgaaa ttccataaaa aacgactgca ggaattattg
    18961 taagaaaagg ataaaaatag ttcagtccat ctccataatt gttaaatgtt gtatagttga
    19021 ttggcccttc aaaaatagaa gataaccctt tgattcgtga aagatgaaaa gataaatcac
    19081 ctgttgtata aatttcttga gcatttctca taaaatttac tgaatataaa gtactaagta
    19141 agataagaac tactaaaaga aatgcttttt tacttatttt catagatttg ttgttctcct
    19201 tttttaattc tattttcagt atactagaat tgtctttttt taataagttt tg
//
LOCUS       DAYYCQ010000055        19248 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000055 DAYYCQ010000000
VERSION     DAYYCQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.055"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            241..2076
                     /gene="tdc"
                     /locus_tag="ACHAYF_001883"
     CDS             241..2076
                     /gene="tdc"
                     /locus_tag="ACHAYF_001883"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8579321.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2381..3859
                     /locus_tag="ACHAYF_001884"
     CDS             2381..3859
                     /locus_tag="ACHAYF_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8579322.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3825..5156
                     /locus_tag="ACHAYF_001885"
     CDS             3825..5156
                     /locus_tag="ACHAYF_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8579323.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5270..7822
                     /locus_tag="ACHAYF_001886"
     CDS             5270..7822
                     /locus_tag="ACHAYF_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8579324.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7829..9268)
                     /locus_tag="ACHAYF_001887"
     CDS             complement(7829..9268)
                     /locus_tag="ACHAYF_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579325.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILEKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9243..9425
                     /locus_tag="ACHAYF_001888"
     CDS             9243..9425
                     /locus_tag="ACHAYF_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579326.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9614..9976
                     /locus_tag="ACHAYF_001889"
     CDS             9614..9976
                     /locus_tag="ACHAYF_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8579327.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10163..11020)
                     /locus_tag="ACHAYF_001890"
     CDS             complement(10163..11020)
                     /locus_tag="ACHAYF_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8579328.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11140..12051
                     /locus_tag="ACHAYF_001891"
     CDS             11140..12051
                     /locus_tag="ACHAYF_001891"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8579329.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12058..13709
                     /gene="mdcA"
                     /locus_tag="ACHAYF_001892"
                     /pseudo
     CDS             12058..13709
                     /gene="mdcA"
                     /locus_tag="ACHAYF_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13811..14110
                     /locus_tag="ACHAYF_001893"
     CDS             13811..14110
                     /locus_tag="ACHAYF_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8579330.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14100..15776
                     /gene="mdcD"
                     /locus_tag="ACHAYF_001894"
     CDS             14100..15776
                     /gene="mdcD"
                     /locus_tag="ACHAYF_001894"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8579331.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15780..16373
                     /locus_tag="ACHAYF_001895"
     CDS             15780..16373
                     /locus_tag="ACHAYF_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8579332.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16397..17473
                     /locus_tag="ACHAYF_001896"
     CDS             16397..17473
                     /locus_tag="ACHAYF_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8579333.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17498..18367
                     /gene="mdcB"
                     /locus_tag="ACHAYF_001897"
     CDS             17498..18367
                     /gene="mdcB"
                     /locus_tag="ACHAYF_001897"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8579334.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18478..19242
                     /locus_tag="ACHAYF_001898"
     CDS             18478..19242
                     /locus_tag="ACHAYF_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8579335.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
BASE COUNT         6163 a         3289 c         4138 g         5658 t
ORIGIN      
        1 ttgtttgttc gcttttttta ggttatctat tttagaagac tgatttattg aaaaattgtt
       61 cgcttttttg cgttgaatta tcagaaaaat ccgactttat ttatttttgt ttgtaaaaat
      121 ctacgtggat ttgatgttat aaaaaaaaaa ttctcatttt tggcgctata gtgaagaggt
      181 gttacagata aacacatgta tctgcaagac attgaccaac aaaatatgga gggaagaaaa
      241 atgaaagata tggatatcaa ggccgtcttt ataggcgaca aagctgaaaa tggaccagtt
      301 tataaaatgt tgttgaacaa gatggtagat gaacatcttg gatggagaga aaattatatt
      361 ccttcagata tgcctgcaat tagcgaagga gataagctca ctccagatta tctagccacc
      421 cgcgaccata tgatagaggt cttggatgaa gtcagtcagc gattacgcgc aggatcgatc
      481 ccatggcatt cagcaggccg ttactgggga caaatgaatg ctgaaacatt gatgcctgct
      541 ttattagctt acaactatgc gatgctgtgg aacccaaata atgttgcatt ggaatcttct
      601 atggcaactt cgcaaatgga agcagaagta ggacaggatt ttgcttcatt attcaatatg
      661 acagacggat ggggacacat tgcagcagat ggttctattg ctaatcttga aggattatgg
      721 tacgcccgtt gcatcaaatc gattccttta gctgtcaagg aagtgctgcc tgaaaaagtg
      781 aaaaaaatgt ctgagtggga acttttgaat ttatctgttg aagaaatttt ggaaatgacc
      841 gaaagcttta cggacgaaga aatggatgag gtcaaagcag cttcttcccg tagcggaaaa
      901 aatatccaaa gattagggaa atggctggtc cctcaaacaa aacactattc ttggatgaag
      961 gcgttagata tttgcggagt cggcttagat cagatggttg ctatcccagt tcaagaggat
     1021 tatcgaatgg atattaatgc tttggaaaaa acaattcgtg aattagctgg acaaaaaatc
     1081 ccaattctag gtgtggttgc tgtcgtagga acgacagaag aaggtcaagt agacagtgta
     1141 gataaaatcg tccaattaag agaaaggtta aaagatgaag ggatctattt ctatttacat
     1201 gttgatgccg catatggcgg ctatgcgcgt tcactgtttc taaacgaagc aggagaattt
     1261 gtgccgtatg cttccttagc tgaattcttt gaagaacatc atgttttcca ccactgcgta
     1321 acgattgaca aagaggtata tgaagggttc agagctattt cagaagcgga ttctgtcacg
     1381 atagatcctc ataagatggg ctatgtccct tatgctgctg gaggaatcgt aatcaaacat
     1441 aaaaatatga gaaatatcat ttcttatttc gcgccatatg tgtttgaaaa atcagtaaag
     1501 gctccagata tgttaggagc ttatattttg gaaggatcaa aagctggagc aaccgccgcc
     1561 gcagtctgga cagcacatcg tgtattgcca ttgaatgtga ccggatatgg tcaattgatt
     1621 ggggcttcaa tcgaggcagc tcaaagattc agagaatttc tcgatcatct gacttttact
     1681 gttaaaggaa aaacaatcga agtctatcca ttgaatcatc ctgattttaa tatggtcaat
     1741 tgggtattca aagaacaagg ctgtacagat ttgaacgcta tcaatgaatt aaatgaaaaa
     1801 atgttcgatc ggtcttctta tatggatggc gatgtttatg gtgaacggtt tattacttct
     1861 catactacat ttacacaaga agattacggt gattctccga ttcgttttgt tgaaagaatg
     1921 ggtctaacga aagaagaatg gaaaaaagaa cagaaaatca ctctgctacg tgcggctatc
     1981 atgactcctt atttgaatga tgatcgaatt ttcaattttt atacaaaaaa aatcgccaaa
     2041 gcaatggaaa agaaactaaa cgaaatcatc caatagaact gatttttgtg tgaaagaata
     2101 acaagagaca tatggagaac agacaattat ttgcttcatc aaaacaattg tatttccccc
     2161 tgtttttcat atattcctcg ccaagatatt cttttttata aaacgttcta taagttggat
     2221 cgttcgtttc atgtatcaaa cccagttcac taagatcgtt caatggatgt ttcggtgata
     2281 ctgggttttg tactgaatcg atgaaacctt gtcatttctg ttcttgaaca aaaataaaag
     2341 aaaacaaaaa catatgtcgc tattgaaaga aggaataaag atgaaagata ctggttcatc
     2401 aaagcaaatc acgaccatga cctttatcgg aatgacctgc gcccttgtag caagtgtacg
     2461 aaatattcca gatgtagcag caacgagctg gacgatgatt ttttacatgc tggttgcagt
     2521 attattgtac gcttttccaa tctcattgat ctctggtgaa tttgctggaa tgtttcccca
     2581 aaaaggtggt ccagaacttt gggtatcgaa tgctctagga aaaaaatggg ggttcgttgt
     2641 ctcatggctg ttatgggtcc aaatgtttcc tgggatggtg atggtcgctt cagcactcgc
     2701 tccgttgttt ggtaatatta tcgataatgt gccgcttggt ttgaacagca aatttacatt
     2761 agttgtcatt ttagtcgttt attggatcat cacttttttg aatttgaaat ttgatatggc
     2821 aaaaatcggc ggaaaagtcg gtgtttggtt aggattgtac gtgcctttga cgatgatgct
     2881 cttattaggg tttgctgcgt ggatcaaagt tgggatcgta ccaaccggaa cattaggcag
     2941 tttttcatgg gacaaattaa taccagatac tacgactgca tcctcatttg tttattttgc
     3001 gccgatcatg tttattttta ctggtatcga aatgtcctct gtgtatatca ctcgattgga
     3061 aaatccagtt aaaacgtata ttcgcggagt atttgccgcg ttaattttta tatttttcgt
     3121 caatattttg aatgctttag ttgtagcaaa tgtcgtacca aaaggacaaa tggaattgaa
     3181 taatatcgcg caatctattt cgatttattg ccaaattctt ggtttgccac atcttattgt
     3241 gaatctgttt agtttacttg tcttcattgg tgtagcagtc caattatctg cttgggcctc
     3301 aggaccagca aagacagtga cagaaagtgc tagaaaaggt gcgtatccac caaaattcaa
     3361 tttttggaag acgaatcaat ttgacgtatc caaatccgtt attttgactc agtctgtcat
     3421 tatctcaatc tttgcacttt tttatttgct gattcctggc gtcaatcaag catttttgat
     3481 gctcgtaaat tcaactactg ttatctactg tatcgtctat gtcatcatgg ggatcgcagt
     3541 tttgagattg cgttatacac atgcaaagtt gaatcgacca tttagaattg gaaaaaaaga
     3601 gaaaaagagc aatcttggag tttggactgt cgttattgtt ttatttgcgg caatcggatt
     3661 ttctgtcgga ctgacgatga aagctggaac atggatcaat ctaattgcag tcacagccat
     3721 cagtgtcatt ttgtttgtag taccactctg tattgaaaaa ataaagaaac cttcttggga
     3781 acaggaagtc gccatgctgc ttgaaaaaga aaaaggtgaa cttgatggaa aaaccggcaa
     3841 tgaaacggac tctaactaat cgaaatatcc agttaatcgc attaggtggc gcaattggaa
     3901 caggattatt tttaggttca gcaaaggcaa ttgcatcagc agggccagct gttattctcg
     3961 tttatttgat atgcggtttg gctatttatg gtttgatgcg ggccatgggt gatttatttt
     4021 tagcaaatag tacatttaac tctatcagtg atttcgttga ctattattta ggcaaaaagt
     4081 gggcattttt tgtcaattgg acttattggc tttgctggct tggagctgga cttgcggaat
     4141 taacagcaat aggattgtat atccatttct ggtttgtaca tgtaccagct ttgataagcg
     4201 gaggtagcgc actaatgatc ttaatggcag tcaaccttgt tgcagtcaag tggtttggtg
     4261 aattggaatc ttttttctca atcatcaaga tcttggggat tttccttttt atcttattgg
     4321 gtataggatt gagtgtctca gattttcata cccagtctat cggaatcaaa gaaaattttg
     4381 gacagatcga tgaattacta ccgtttggct ttgctggaat ggtagtctca tttcctatgg
     4441 ttttattcag ttttgctgga attgaaatga tcggcttaac cgttggcgag acaagtgatc
     4501 cgcaaaagaa tttgaaaaaa gcaatcaact gtctgccttg gcgtattctc tttttctata
     4561 taggaacgat tttgtcgatt ttatttgttg ttccatggca ttgtttagat ttaaaagaaa
     4621 gtcctttagt gactatgctg caagtgatcc attgccaagg cggagcagca attgtcaatc
     4681 taatgatttt ggttgcttct ttgtcctcag caaatagtgc agtctacagt actagcagga
     4741 ttttatatca aacagcaaaa gaaaaaagtt tgccccattg gttttgtgcg ctttctaaga
     4801 aaggcgtacc tttacacggt attttaatta caggaatgtt attttttgcc ggtctttgtc
     4861 tacatttttt tgtagctgat agtcgattgt tttttcagct tttggcaggc atgacaacga
     4921 gttgttttct atttgtgtgg tcttcgatct tgtgtgcaca tgttcaattt attaaagaaa
     4981 agaaacggaa cactacacaa agatataaag atcttgcttt gttggtgttt tttacttcaa
     5041 ttgctttcag cttgttattc gaaagcacga cacgctttat tcctttttta ttggttatct
     5101 ggtttgtatt cttaaatttt gtatataaaa gaataagcaa tagaaatatc agttaggccc
     5161 agatacattt tgcaaaacga attatttcag acccccctga aataatcgat acgcctgaaa
     5221 aaacttccct gcgtataata acagtatcaa ttaaacagga ggggatagga tgaactggta
     5281 cagattgacg gtagaagatg tattgaaaaa ctatcaaaca tcaacagaag gcttgtcgca
     5341 agaagaacga cagaaacgcc tgactgatca aggatataat gaaataaaag caaggcagca
     5401 gacaaaacga tggaaaaaaa tagcaaagca ttttactgat ctgctgatga tcgtgttgat
     5461 cattgcctct tttttgaaat ttgcttctag tgaatatatt gaaggaagta ttattttatt
     5521 cgtagtgatc gtcaatggac tcgttagtta ctggcaagaa agaaaagcgg aagaatcatt
     5581 gaatggtttg aagcagctga tgggacaaga agcgatagta ctatcaaacg ggatgcaaga
     5641 aaaaatccct gcgaaagaac tggtaaaagg agatgtcatt gtcttacgtg ccggagatgt
     5701 cgttccagca gatatccgat taatcgaagc acatgattta ttagtggaag aatcgatttt
     5761 gacgggagaa tcagaagcat ctgaaaaaag tcatgccgtt ttgacagaag aagaatcgat
     5821 cggcgaccag aaaaatatgg gtttttcagg aacgcttgta caatctggtt ctgcagtcgg
     5881 tgtcgttgta gaaacaggaa acacgacaga aatcggtaaa atcaatcaag cattgcaatc
     5941 ggtcgaacaa caaacgacgc ctctgattaa aaaaatcaac cgattaaaca aacaaatttt
     6001 tcaaggcatt ctttgtttga gcctattctt agttattttt acgacatttc gatatggtat
     6061 ggattggcat atccttcttt cggctacgat tgctttggtg gtgtcaatgg tacccgaagg
     6121 attgccagct gttttgacca tgattctatc cgttggagta catgaaatgg caaaagaaaa
     6181 ggcgatcatc aaaggacttc cttcggtaga aacattaggt tctatgacag tgatctgttc
     6241 agataagaca ggaacattga caaaaaatga aatgacagtt gtggatgtag ctgcaaaaga
     6301 agaagcctgc gtgttgtcca ttatgaaaaa ctgtcaggaa ttaaaaacaa aagaagaaca
     6361 gaaaacagaa aatttaagtg gcaatccgac agaagttgcg ctgattcgac acacagaaac
     6421 agccaatcta cctttgaaaa aaatagaagc aaagattccg ttcagttctg aatacaaata
     6481 tatggcaacg atgcatgcgg aggaaaaagg agcggtgatt tacgtcaaag gtgcaccaga
     6541 agtattattt gctaaatcaa ctctttcttc tgcagaacaa gaagcatgga gtcagacagc
     6601 tgcagaattt gcgagaaaag gtcagcgtgt tttaggattt gcttataaaa aagtagattc
     6661 gaaacaggaa ctcacgcatg agactttaac ccaattaact tttgcaggga tcgccggatt
     6721 gatcgatccg ccaaaagaaa gtgcagtcaa agctgtaaag gaatgccaac aagcagggat
     6781 ctcagtgaaa atgattacag gggatcacaa agacacagca aaagcgattg cggaacaaat
     6841 tggcttaaaa catactgcca aagttttaga agggatcgat ttggatctta tgtctgatga
     6901 agagttgatt cagcaagtgc caatagtcga tgtctttgct cgaacgacac cagaacataa
     6961 actgcgaatt gttaaagctt tgcagaaaaa tggagaaatc gtcggaatga ccggagacgg
     7021 cgtaaatgat gcacctgctt tgaaaagatc cgatgtagga attgctatgg gaatcaaagg
     7081 tagtgaagta agcaaacagg cagctgatat ggttttagga gatgataatt tccatacgat
     7141 tgcaaaagcc gtaaaagagg gccggcggat tttggataat ttgcaaaaaa ccatcaattt
     7201 cttcttgcca actgcattag ctcaaggatt gatcttgatt tgggcactta tgctgaatcg
     7261 cccgctacct ttgtccccag tacagatttt atgggtcaat atggtaacga caatcacttt
     7321 atcttatgca ctagggttcg agtcgccaga tccagaaatc atgaagcgcc caccaagaga
     7381 tccaaagcaa ggaatcctat ctggttatca tttgtttcgg atcatctatg tctccctact
     7441 gatcatggtt cctgcctatt tattggctat gcagtttgaa gggcaaagct tacagcaaac
     7501 cattcttctg caaaatatcg tcttagctca agctgtttac atgatcaatt gcagagagtt
     7561 gtccaagccg tcattgaacc aaggtctttt gcaaaacaaa gcgctttttc tttcattagg
     7621 tatccttgct ttattgcaag ggatggttat ttttctacca gtcggccagc agctgatcgg
     7681 tacagtaagt ttaacgatga cacaacagtt attgattgga gcaaatgtga ttcttctatt
     7741 cttagttgta gaaatagaaa aagggatcat gaacaaactg tttcgcaaaa aagaaaaaac
     7801 agctagagaa tacagtcgct agccgttact ataaaaaagt tttgaaagtg agttcagtca
     7861 aacgattgag ctcatttttc gttggaatag aagaaatgta tacaataggg atatctagag
     7921 aatctagagg gaaattggaa atcaagatat ctgttttttc aaaagaaata gtggaaagat
     7981 cggttagttc gataggtaag aagcagacac gttttcctaa gtaatctttt aattcctgct
     8041 gcaagaacgc cttccatgaa ggttctcctt ggaaaataaa atagacattg aacaaattcg
     8101 gtttgataga aaaaatcgtt tgttggatca gtagagacaa gagttggaga aagtaatttg
     8161 gttcattgat ttctatttct ttttttagat cgttcagtaa attctctttc tgttgataca
     8221 agtgtgcata ttgctgatca tatgtttcta aaaaacgatg gtattgacgg tccaatgctt
     8281 caatcaatat cggcgattca tgatatttta atatgaggag agccaagtta tcagtcagag
     8341 atgttttttt tagttgatca agagacagct ctgaagctag ttcctctaca aacgtttcga
     8401 ggacctctaa aaatggagaa tgatgcaggc agcgagcggt ttcttgtcca tagttattgt
     8461 tgtaattcca actttccaga aagcagaaag aagcaaaaga aagctcagtg tctttaagat
     8521 aaataccttc cgttaaatac aattttttca atgcagagtc atacagggag gagagtgtgc
     8581 tgttggtatg gatagaagaa tctaatcggc aattcgcttt tatacggatc gtattgataa
     8641 agaaccaaca tgttccgaaa atctcttctg tttcaaccgc tagttcagtt tgactgaggt
     8701 ttcttaaaaa ttgaaaataa tgggattcat ggatcgaata atcctcgaaa aaatagttgt
     8761 tatgagtgaa aggaatcagc aatcgatgat aaaaagtacg aatcgcacgc tctttttcaa
     8821 gaatacaagc tgtatatttc gtcaaatcga tctgcaagtc gtaacttgct aaacgctgat
     8881 tgattttagt cacatgtctt ttcaatgtcc cataagaaag cccatggtcg gctagaaaat
     8941 cagggacagc aacttttttt gtaaaaagca gatttttcag cagttcgaca ctgactgatt
     9001 gtttgaaaaa aatctcccaa aggtcatttg ttaataaatt ttgctggttt tgcaaactga
     9061 ttcctgcttg atcgctgatg atgtcccagc cttcgggcag ctgagagcga atagattgaa
     9121 ggtcagaaaa aatcgttcgt tcggttgtcc ctatgatttc agctaattca tttacggtta
     9181 tctttgtatg attcagcaat tgttctacta aagaaacatg acgtcgaata tctttttcgg
     9241 taatgatttt tttcattagt gaatacatca atcagactcc cttctattct aagaatacgg
     9301 agaaaataaa cggtggtcaa gtttcaactg atttttcaat cattgtttat ttttctggag
     9361 catttactga tgataagaat ttcatcatga acttgaaaaa tgataccact aaaatcaaaa
     9421 aataggtggg caaacaataa taaacatttt aatagtagat ggtagcaaga ccaacacagg
     9481 cggattgaac atttaagagt tgataaaaaa gaaaaatatc gaagtatttg attagcaact
     9541 acttgtattc atttttctat tcttacgtgg tatctttgaa taaagcgaat caataagaaa
     9601 gagatggaag cagatgctaa cgaaaaaaga actaatcgat cacatcaatg aaacatacaa
     9661 tttgtctcca gaatatattt ttaaaaaatt tcctgattat tgcgtattca gacatcaaaa
     9721 aagcaaaaaa tggtttgggg tcctgatgtc tgttccagaa gaaaaaatct atgatgatgc
     9781 aagtgaccat aggatcatca atattcttga tttgaaggtc gatcctgaac ttggcggaat
     9841 tttgcgagga aaagaggggt attttccagc ctatcacatg aataaagaac attggatctc
     9901 tattgatcta cgtaaaatag aaaagctaga tgaactagca gatttgattg atggaagttt
     9961 taaatcaacg caatgaatgt ctaggacaat agagaagagg ttgtgacaga aatgtttagc
    10021 ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa atttttggga aatttcggct
    10081 tattttctaa gaaattactt ctggaacacc gtttgttcga aaaaagagct gcgacaagaa
    10141 ttttgtccca gctctttttt ttttattttc ctaaataaaa ttcccgcatc gtttttgcaa
    10201 tcattttgat caaaggatga tcttctgtga agctagaaga taaataaatt gttcgtgtca
    10261 agggcaaatc tttgatttcc cgcaagacga aaggatcatt tttatcgtaa gaccaagaaa
    10321 cttttgggaa aaagctaaca gctaatcctt gcttgacaat agaacgtaat gtcgcaggat
    10381 catctgtcgt cgaactgatc gtcaattcga tacttttcgc ttcaagtgct gcatccagtg
    10441 tatgacggaa ggcgttgctc tttgacagca tcaagatggg ggtgcgttgg atatcttcta
    10501 attgcagatg ttctttttta gcaagaggat gtttcttagg aatggcgaca taaacaggtt
    10561 ctttcatgat aggaatattg gtcatgccag gcttcatctc cgaagtgatg tacagtacag
    10621 gatcggtaat atcatctggt aaatgatgtt gcatgatcgt taattggata tcaggatgct
    10681 tttccataaa aattcggacc aagttcggaa tagaaacaga ggctacctct aaatacaata
    10741 tgacggaagt aacgatcttc tcattgaccg aagacaataa tttgattcct tcttctaatg
    10801 tgtctaatgt cttgactgca tatggataaa attcttctgc ataaacggtc gtctcaatgt
    10861 ttctgccgct atggtaaaac aaagggacac ctaattcgtt ttccaaatgt ttgatcgcac
    10921 ggctcaaggc aggttgagaa ataaataatt ctttggctgc ttgggtaaca ttttttgttt
    10981 cgaatacttt tttaaaatac tgcagatgaa gcaatttcat aattatccat cccatccata
    11041 cctttttgtt atggtttttt ctttttttgg cattttttat tatagcagaa attgactaag
    11101 atagcagtgg aaaaagtaag tgctgaggag gcgaggctca tgagtacagg tttcttattt
    11161 cctggacaag gcagtcagaa gttagggatg ttagcggata tccctgaaat gtacatggaa
    11221 cgtttagaaa atgtgacggg gtatcggtta gttgataaag aagaaaacta tcaagatacg
    11281 gtttttatcc agctagcatt actgacaaaa gcagtatttt atttagatga aatggaaaaa
    11341 aacggtattc agcctgatat tgcagcagga cattcgattg gtgctttttg tgcagctgtt
    11401 gcttgtcgat ctttgagatt tgagaatgca gcggaactag tctatcaccg cgcagtctta
    11461 atgaaagaag cttatccaag cggttatgca atgggcgtag tagtaggtgt gacgagaagc
    11521 gaagctgagg aaattgtagc aaaaacattt gatcctgacc atccagttta tctgtcaaat
    11581 gaaaattgtc cgatgcaaca tacgttatct gggagtatcg aaggtttatc cgttacgttg
    11641 gcagaagcca aacggcacca agcgcaaaca gcgaagctgc tgaaagtccc tgtgccatct
    11701 cactgtctct tgatgaatga tacggtcaag aaattcaagc cgtatatgca gtctgtccat
    11761 ctatctgatg ctagatgttt gtatataaaa aatacggatg gaagagccac tcaggacgca
    11821 gaagagattc gtaaagattt gttggaaaac ctagctttcc ctgtgcagtg gaaccaaatg
    11881 atggacttgg caaaagaatt aggaatggat ctgacgattg aatttccccc cggcaatact
    11941 ttgacaaagc tgattcatac aaaatttggt gtagataaag ggatccgtac gatcaatcta
    12001 gaccaacatg gtattgatga cgccacgttt ctatatcaaa aatggagatg actaattatg
    12061 gaaaaaccaa accgcaactg ggcttctaaa cgcgaaaaaa aagcgcagcg attggaaaaa
    12121 atcagcaact tgctcaatgg caagtatgta gaaactgaaa aaatcgtaga agtaatggaa
    12181 aaactgattg ctcccggcga caaagttgta ttagaaggcg acaatcaaaa acaagcaagt
    12241 tttctttcag aaagtcttga aaaagtagat gcagaaaaag tgaatgatct gcatttgatc
    12301 atgtctagta tctcacgacc agaacatttg aatatttttg aaaaaggaat cgcaagcaag
    12361 atcgatttct cttatgctgg tgcacaaagc ttgcgtgtcg cccaaatgat tgaagacggc
    12421 acaatgaaat taggtgatat ccatacttat ttggaacttt atggacgctt attcatcgat
    12481 ttgattccaa atgttgtttt agtagctgca gataaagcag ataaagacgg caacttgtac
    12541 acaggtttca atacggaaga aacaccaaca atcatcgaag ctgcagcttt caaagatggt
    12601 atcgtgatcg ttcaagttaa cgaagttgtc gattctttac cacgtgtaga tatccctggt
    12661 gactgggtag acgttgtcgt gaaagcagat aaaccttatc aattagaagc tttattcaca
    12721 cgagatcctc aaaacatcac agaattgcaa attctaatgg caatgatggc aatcaaagga
    12781 atctacgcag aacacggcgt acaatctttg aatcacggta ttggattcaa tacagcagct
    12841 atcgaattgt tactgccaac ttatggtgaa tcactagggt taaaagggaa aatcgcgaag
    12901 aactgggtat tgaacccaca tccaacgatg attccagcca tcgaatcagg ttgggttgaa
    12961 agcatccata gttttggtgg cgaagttggt atggaaaaat atattgctgc tcgcccagat
    13021 gtcttttttg tcggtaaaga cggcacgatg cgcagtaacc gaacattatc acaagctgca
    13081 ggacaatatg ccatcgatat gtttatcggt tcaaccttgc aattagatta cgaaggaaac
    13141 tcttctaccg taacaaaagg acgtttatct ggttttggcg gtgcgcctaa catgggacat
    13201 aatcctggcg gtcgtcgtca ttctacacca gcatggcaat cacttcgcga caatgatgat
    13261 ccattaggga aaggccaaaa attagttgtt caaatggttg aaacatatgg ttcaaataaa
    13321 aaaccagtct ttgtcgaaga attagatgca ctagctgttc aaaaacaagc aggcttagcc
    13381 aatgcaatgc accagtgatg atttatagcg aagatactac acatatcgtg acagaagaag
    13441 gaattgctta tttgtacaaa gcaaaaacaa aagaagaacg acaagcagca attgcagcaa
    13501 ttggcggtgt tacacctctt ggaatgacta gcacgaaaga atcactagat agcttgcgtg
    13561 cagccggcat cgtgaaatta ccagaagatc taggcatcaa acgtagtgaa gcgaaacgtt
    13621 cattattagc tgcacaaacc atcgatgatt tgatcgaatg gtcagatggc ctgtatgaac
    13681 caccaatgaa attcagaact tggtcataaa attggcgaga aaaacctttt gtcgccattt
    13741 acccagatgg aagaagaaag acgaataagc agaacgtgta tgctctgttt tacaaaaagg
    13801 ggagagatcc atggaacaat tagcttataa atatccaacg aaacagccga tcaataagaa
    13861 aatccatgta ggagtggtcg gttcaggaga tttggaaatc ttgatgtttc caacagaaaa
    13921 aacagaatct actgtaaaaa tttgtacagg aagcgatggc ttcggcgaag tatggaacaa
    13981 tgtattaacg agattttttg atcgttatcc aatttcagca gatatcgtga tcaatgactt
    14041 cggtgctaca ccaggagttg tgtacctacg attgacgcaa gcaatggagg aactttcaga
    14101 tgaaaaatag ttttgtagaa ttaaacgcca gagaacgagt gaacgctcta cttgacaatg
    14161 gacgtgaatt gattgatcca ttcgatcaaa tcatcgcgcc aagtttacca gcacaaggaa
    14221 tcgtccctga aagtgatgac gggatcgttg tcagccgtgg cacgatcaat ggcaaacaag
    14281 cagtagtaat cgctatggaa ggaaaattcc aaggtggcgg tatcggtgaa gtttccggtg
    14341 caaaaatcgt tgctgcactt gaacatgtgt tagaagaaaa taaaaaagga aatgagatct
    14401 ttccagtcat tgtactagat acaggtggtg ttcgtttgca agaagcaaac tacggactgt
    14461 tatctatttc agaaatcgga aatgtaatca ttgctctaaa acaatatgta ccagtcatcg
    14521 gtttgattcc aggacgtgta ggatcatttg gcggtatgtc gattacttca gcattaatga
    14581 gttatttaat cgcaacaaaa aaagcgagag tcggtttgaa tggaccagaa gttattgaac
    14641 aagaagcagg cgttcgagaa tttgattcta gtgacaaaga cttgatttgg aatacattag
    14701 gatctcgtca acgggtagaa tcaaaaatca ttgatgaatt agtgactgat agtgtagaag
    14761 caatcaaagc tgctgtcatc gcagcaatgg acgaacgaaa agacacgcat cgcagtgaaa
    14821 gctcagcatt ttatttatca ttacttgatt ctttagatct ttcaaaacca atggccattg
    14881 acgattacaa tgaagcaatt gctaaccatc aagcaaaatc aattgactta ccagaagtag
    14941 cagaaggaaa tgaaccagct gcagcaagtg ttggttacga ttggtttgcg gcactaactg
    15001 gcatgaaaaa tccaacttct tctattagta ctgtgtatta tggtaaatca agtaaattca
    15061 tagaagaagc catcgtgaca tcaatcgtac ctgatcctaa aaacccgatg taccgtgtac
    15121 gaaacggtga agttggttta gttgaaggtt tccgcatggg acaaattttg gatcacgttt
    15181 acgaagaaga taaagacaaa aaagtgaaac ggccaatcat cgttgtgatc gatgtaccaa
    15241 gccaagcata cggctataac gaagaattga tcggtatcca tgtggctttg gctaatagtg
    15301 ctgctgctta tgcaaaactc cgtcaagctg gacatccagt tattggattg attgcaggaa
    15361 atgctatttc aggtgcattt ttagcacatg gattacaatc aagccgcttg attgctttaa
    15421 acaataaaga aattaccgtc caagcgatgt ctaaagaaag tgctgcacgt gtgacaaaac
    15481 gttcggttgc tgaaatcgaa gcagctgctg aaaaagttcc agctatcgct tacgatatca
    15541 ataattatac aaaactagga gcgatctatc atttcctaga agatatcacc gaccaagctg
    15601 cttcagaaca aaatgtcagc aaagtcatcg aagcaatcaa tgaagccatt caagatgtcc
    15661 gcaatacaaa tgacacgatg ctgacaaatc gatacacaaa cgaaacagcc aaaacattcg
    15721 gacgtgtcga aacaaataaa gtccgtgcca aaatggacga agaatgggat gcataaaaaa
    15781 tgattcaagc acatgatatt gtcctttttg ataaacatga aatagaaggg ttcaccttgc
    15841 ctgaatgggt cgttctaact gaaaagatgt tcggaacagt tcgtcgtgcg tcgacggatc
    15901 acgcagaaat actaccgatt ggtttacgtg gacctgcccg caatcagaga tggtcaacag
    15961 aaatctcaaa acatgccgtc aaacaagtca ttcatccgtg ggacatcatc gaacaagaat
    16021 cgtatagaca agagaaaatc atggattatc ctgtttatca gcaatttcat gctgctagaa
    16081 acatattatc aggatgtaaa tggggaatcg gcggcagctt aggatttgaa ttatcgactg
    16141 ggattccggc agtaaaagaa accagtgatt ttgatttgct gctgtacgca aattcaccga
    16201 ttgaattgcc aattcaggta attcaatcgc atcctgcttt ttttgagcaa ttcgataccc
    16261 aagtagtgac tcggcaaggc ggtttttcac taaaagagta tttgagagag ccagagaaaa
    16321 aaattctatt gaaaacagtc gaaggaccta aattaaccaa agaactttgg taataaagaa
    16381 aaagagagag aaagagatgt ttgaaaacaa aaaaatcaaa atcaacgtaa gtgacttcca
    16441 gaaagtatca gctgtaacgg ctgttatgat gcaaacgata ttgagctttg ctttggcaaa
    16501 cagccacagc gcaacaaata cagcttttct tggtaccttt tacgtcatcg ttaaatacac
    16561 agcaccaatg tttatttttg caattgttta taatatggta aaaacaagtc agcatttgtc
    16621 ttattgggaa tttttgaaag aaaagttttt tgaactagtc gttccgtatc ttctatggac
    16681 gacggcttat ctttggctat ttccagcagt ccaacaacaa actccgtata cggatactgc
    16741 aagcttttta ctgaaatatg tcacaggtga tggagcgcct catttatggt ataccgttat
    16801 gatgctgcag atccaattgt tcatgccgtt ttttgtttgg ctcggttaca aagtatttgc
    16861 taataaaaaa tatgtatggc cagtattgat tggttcaacg attttatatg tcgcatggta
    16921 tctgttctat caaacacaag tattgactgg gccttatcat gacagctggt acttattaga
    16981 tcggtttgtt gctagtttca tgatttatgg ggtagccgca tttgtttatc acgaaaaagt
    17041 ctatcaatat ttggacagag tacgttatct tttcttacct gttgccttag ttatcgcgtt
    17101 cttttctgtc cgttcattgc tcgcacatcc aggagacttg agctttgcga atgcacctta
    17161 cttgaatacg atccaaagtt tatacagttt agtcatcatc tttgctgtct tcataggcgc
    17221 ttccaaaatg attgtaaacg attcgccaaa attgccattg ttcaaatggt tgtctgttta
    17281 tgcttatcgt acgtatttag ccaatgtatt tgttttccaa gtactgttat tattgtttaa
    17341 agatagttgg ctgcagttac cttcaggagt catgattctt gtagcttatc taatgacggc
    17401 aagttgtgca tttgctttaa gctggttact tcatatcatt tgggtagcta tcaaaaaagg
    17461 tttcagcaag taatagtagg aagaaggagt gagtgtcatg ataaatgacg aacgaaaaaa
    17521 agcagtgact cagcaactcg ctgcgttggt agaggaagca ttgatcgcag aagtaacatt
    17581 aagtccaaaa cctggattag tggatgcact ggatgcgggt gcacattccg atatggacta
    17641 tgctttattt cttaagtctg ctaagacgct aactcctttt tttgaagaaa tggctcaaat
    17701 agcatggcat catgctattg atcaggagtt aagagaaaga attgctgaga ttgggcgaaa
    17761 agcggaaaag gcaatgctga ttgctacaaa tggcatcaat acccataaag gagcgatttg
    17821 ggcaatgggg ctattgatca gtgtcattgc acaaaaaatc agtatggaac agcctttttc
    17881 tttatcgaaa atacttgatg atgtcagcaa gcttgctgct tttcctgata caaaatatcg
    17941 tcataaaaaa accactcatg gtcatagggt gaagcaaaaa tatggcgtaa atggtgcata
    18001 tgaagaagcg atattagggt atccgcatat caggcttgca attcaagcag ctaaggagct
    18061 agataatgat tcatatgtac agaaacaaat gcatatgttg ttagttttga tgagttcgct
    18121 agatgacaca tgcgtattgc atcgcagtga tcagaaaacg ttagtggaaa tgcaaaaact
    18181 tgctcatgct gctagtcaaa gtgctttacc aaatcgtcca tttacagcac tgttgcactt
    18241 ttgtcaggaa aaccaaatct ctcctggagg aagcgcagat ctcttgtctg caagcttgtt
    18301 catgctgaag atagaggaat tatggactgc cgacacatac atttgtccga tgttactcaa
    18361 tgactaaata agacaaaatt gctaaaaaaa cagtttatgc atctggttat tgactcttcc
    18421 ttttagcaag gctatcataa aaatagtttt ttgtaataaa agataacgag gaagacaatg
    18481 aaaaaaatca attatttagc aatcattttg ttagtgggcc tatgtttgtt tgctctgccg
    18541 acccaaggat tcgctgtcgc gggtggtcat ggtggaggta catctagcgg tcgaagttcg
    18601 ggcagaggtt catttggtgg tcgttcatat gggggcagtt attttggcag tcgttcttac
    18661 ggcagagatt cttatgggta ttatggtcca ggtacaggtc cttctagtta tgagcaaacg
    18721 ggatttcttg cattagctgt tttgtcagta ggaggggttt ctgctttttc tatcgtcaag
    18781 aataaaagac aaaagaaaag gctgaaagcg gagatattgt cccgcatgtc aggaacggct
    18841 aaagaaaaga aagcacagtt tctggaaatc caaaagatat tcatgaagat ccaatccgct
    18901 tgggaagaag aaactcctga aaaagccaga agctgttata cagacaagct gtttgccgaa
    18961 catcagaaag ttctgcaaaa aaacaaagca gacggtgtaa gaaatcatac taaaaaaata
    19021 actattcagg aagtaggaaa ttatcgacag atccacgaaa atagttttgc catccgaatt
    19081 gatttcagat gctgtgatta ttcagtcgat cgaaaagatg ggcgattgat ctcaggaagt
    19141 aagacacaac gacagtattt ttctcagata tggtattttg atttttctga gaaagaaaaa
    19201 agatggcaag ccgattttat tcagccgatt atgctggatt gacaggaa
//
LOCUS       DAYYCQ010000056        18559 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000056 DAYYCQ010000000
VERSION     DAYYCQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.056"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..801
                     /locus_tag="ACHAYF_001899"
     CDS             154..801
                     /locus_tag="ACHAYF_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8579336.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    930..1146
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1194..3839
                     /locus_tag="ACHAYF_001900"
     CDS             1194..3839
                     /locus_tag="ACHAYF_001900"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8579337.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4149..5462
                     /locus_tag="ACHAYF_001901"
     CDS             4149..5462
                     /locus_tag="ACHAYF_001901"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8579338.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5452..6105
                     /locus_tag="ACHAYF_001902"
     CDS             5452..6105
                     /locus_tag="ACHAYF_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8579339.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6160..6852
                     /gene="radC"
                     /locus_tag="ACHAYF_001903"
     CDS             6160..6852
                     /gene="radC"
                     /locus_tag="ACHAYF_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8579340.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7002..7202
                     /locus_tag="ACHAYF_001904"
     CDS             7002..7202
                     /locus_tag="ACHAYF_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8579341.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7456..9135)
                     /locus_tag="ACHAYF_001905"
     CDS             complement(7456..9135)
                     /locus_tag="ACHAYF_001905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8579342.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9137..9349)
                     /locus_tag="ACHAYF_001906"
     CDS             complement(9137..9349)
                     /locus_tag="ACHAYF_001906"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8579343.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9742..11472
                     /gene="efrA"
                     /locus_tag="ACHAYF_001907"
     CDS             9742..11472
                     /gene="efrA"
                     /locus_tag="ACHAYF_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8579344.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11469..13229
                     /gene="efrB"
                     /locus_tag="ACHAYF_001908"
     CDS             11469..13229
                     /gene="efrB"
                     /locus_tag="ACHAYF_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8579345.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13324..13521
                     /locus_tag="ACHAYF_001909"
     CDS             13324..13521
                     /locus_tag="ACHAYF_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579346.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13674..14159
                     /locus_tag="ACHAYF_001910"
     CDS             13674..14159
                     /locus_tag="ACHAYF_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8579347.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14263..14856)
                     /gene="clpP"
                     /locus_tag="ACHAYF_001911"
     CDS             complement(14263..14856)
                     /gene="clpP"
                     /locus_tag="ACHAYF_001911"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8579348.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15036..15563
                     /locus_tag="ACHAYF_001912"
     CDS             15036..15563
                     /locus_tag="ACHAYF_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579349.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15654..16391
                     /locus_tag="ACHAYF_001913"
     CDS             15654..16391
                     /locus_tag="ACHAYF_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8579350.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16395..17312
                     /gene="miaA"
                     /locus_tag="ACHAYF_001914"
     CDS             16395..17312
                     /gene="miaA"
                     /locus_tag="ACHAYF_001914"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8579351.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17314..18543
                     /gene="hflX"
                     /locus_tag="ACHAYF_001915"
     CDS             17314..18543
                     /gene="hflX"
                     /locus_tag="ACHAYF_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8579352.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5995 a         3210 c         4033 g         5321 t
ORIGIN      
        1 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gttttatttg
       61 tctcacaccc ttttccttcc tacttgtaac cttgctcccg tgttagaaac atgttacact
      121 aaatatgtga aattattaac aaggagcgtc gttatgcgag aaaaaacaga tacactgcca
      181 aaagcaactg cgaaaagatt acctttatat ttacgctact tgaagttgct ggatgactca
      241 ggtataagta gaatcaaatc aaatgagttc agcgaaatca cccaaattcc ttctgcaacg
      301 atccgcagag acttctcgca attaggcgag ctaggcagaa gcggatatgg ctatgatgtc
      361 ccttttctta tcgatgtatt caataatatc cttaatacta aagaagaaaa acgaatcgca
      421 ttagtaggat atggaaatct tggcaaagca ttgaaacaca acaattttag aagaaatgaa
      481 aatctaaata tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg agtaattgat
      541 ggagaaatga tctatccgat tgatcgtttc gcggagatag ccaaagcaaa aaatgtgaca
      601 gtagcaatct ctacggtacc aagtaaatat tcacaatctg ctattgatga aattgtcaaa
      661 ggaaatgtaa ccgctatact taattttgcg cctgacagag taactgtccc agcttatgtc
      721 aatgttcaat acattgattt gacaaccgag ttgcagacgc ttatttactt tgatgagaat
      781 tactcagaag ttttttctta ggaaaatttg agtaggctta ttgatcttgc gagaactcgt
      841 cgtaattctt tcaaaaattt tttagtacta aatgaagtgg ttttttcacg accacttgtt
      901 gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct gaacaagagg agtaactggc
      961 acattcattc ttagagagag aactatctgc tgaaaggttc tcaatgacaa ccagtgaagg
     1021 tagcttggga gtttctgtct tgaatggagt agagatagac gagtcgtgat cgttaactca
     1081 cttgagaggc aataatgatt gattattgca atttaggtgg taccgcgtaa atcatacgtc
     1141 ctagaatacg aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac agcatgacgg
     1201 aagaaaataa cctatcaaca aaatataatc cgcaagaagt tgaagctgga cgttatcagc
     1261 attggttaga acaagatttg tttaaaccga gcggagataa aaaagcaaaa ccttattcaa
     1321 tcgtaatccc gcccccaaat gttactggaa aacttcattt agggcatgct tgggatacga
     1381 ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg atatgatact ttatggctgc
     1441 ctggtatgga ccatgcagga atcgcgacac aagcaaaagt agaagcgaag cttagagagc
     1501 aagggatttc tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg tgggaatgga
     1561 aagaagagta tgcgagccac atccgcgaac agtgggctaa aatgggctta tctttagatt
     1621 acagtaggga acgttttaca ttagatgatg gattatctga agcagtacgt aaagtttttg
     1681 ttaccttata cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac tgggatcctc
     1741 aagcacgaac agctctatct gatatcgaag ttattcataa agatatcgaa ggcgcctttt
     1801 atcatatgag ctatgaacta gcagatggat caggcgtagt agaaattgcg acaacccgtc
     1861 cagaaacgat gttgggagat acggcgattg ctgttcatcc agaagacgag cgttatcaag
     1921 cgctcatcgg caaaaaagtg attctacctt tagtcaataa acaaatccca atcattgcag
     1981 atgaatatgt agatatggaa ttcgggacag gtgtagtaaa aatcactcca gcacatgatc
     2041 caaacgactt tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt atggatgatg
     2101 atggtacgat gaatgaacta gcaggaaaat atgctggaat ggaacgtttt gctgctcgta
     2161 aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat taaaatcgat acgatgatcc
     2221 acagtgtggg tcattccgag cggacaggtg tcgttgttga acctcggctg tctactcaat
     2281 ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga taaccaagaa acagatcaag
     2341 cagtggaatt cttccctcca agattcaatc aaacgttttt acgttggatg gaaaatgtcc
     2401 atgattgggt aatctcacga caattatggt ggggacatca aattcctgca tggtatcaca
     2461 aagaaacagg tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa aactgggtac
     2521 aagatcctga tgttttagac acttggttta gttcagcttt gtggcctttc tcaacaatgg
     2581 gctggccaga tacggaaagc gaagattata agcgatactt ccctactagc actttagtaa
     2641 ccggttatga catcattttc ttctgggtaa gcagaatgat tttccaaagc ttggaattta
     2701 ctggcgaacg tccatttgag aatgtcttga ttcatggatt gattcgtgac gaacaagggc
     2761 ggaagatgag taaatcatta ggtaatggaa tcgatccgat ggaagtaatc gaaaagtatg
     2821 gagcagatgc tctccgctgg ttcttatcga atggttctgc accaggacaa gatgtccgct
     2881 tcagctatga aaaaatggat gcttcttgga actttatcaa caaaatctgg aatgcttcac
     2941 gtttcgttat catgaatatc gaaggaatga ccgtagaaga gatcgattta tctggagaaa
     3001 aatcagtggc tgaccggtgg atccttactc gtttgaatga aacgatcgaa cgagtaactg
     3061 agctatttga tcgttttgaa tttggtgaag caggtcgaca actgtataac tttatctggg
     3121 atgatttttg tgactggtac atcgaaatga gtaaagaagt actttatgga gatgatgctg
     3181 tttcaaaaca aaccacccgt agcatcttag tccatacact tgatcagatc ctacgtttat
     3241 tgcatccaat catgccattc gttacagaag aaatatggga acatattcct catcagggaa
     3301 attctctagt agtggcagaa tatcctgttg tacgtccaga gtttgatgat gaaacagctt
     3361 ctaaagggat ggaagtattg aaagaattga ttcgagccgt tcgaaatatt cgttcagaag
     3421 tgaatacacc attgtctaaa ccaattacgt tgctaattaa aacaaacgat gctaaaatcg
     3481 atcaattttt agttgaaaat acaagctata tcgaacgctt ctgtaatccc gaagagttga
     3541 cgatatcaag tgagatcact gcgccagatc tagcgatgtc agctgttctg acaggtgcag
     3601 aaatcttctt gccgttagca ggattgatca atattgaaga agaaatcaaa cgattagaaa
     3661 aagaactgga aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt tctaatgaac
     3721 gttttgtttc taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa gaaaaagatt
     3781 atttagagaa gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact attcaataag
     3841 aatagaaaaa gagagtgtga aatcagctag ctattactaa aagctagctg attgaacatc
     3901 gttacaggaa agagtttgag cctcactctt agcttcgatt cataaggaga tgtgacataa
     3961 attttgtcgc atctcttttt tagtctactc ttattatgta ggagaaggag ggaggtggat
     4021 catgtatcaa gagtcaataa agtagttgat aaaatcgttc ctctctctgt ttcttttctc
     4081 tttgtcatac ttttattttt cttatgtgtt ctatttgtta taataaaaga gaaatggggg
     4141 aattgttgat gacgattgaa gaagcaatag aatggatcca tagtcgatta ccatttggtt
     4201 cacgtcctgg ccttgaccga gtcgaagctt tattagaagt ggtagggcat ccagagagaa
     4261 aagtaaaaac gatccatatc gctggaacaa acggaaaagg ttctactgta gcttatttga
     4321 gaagcctatt agaagaggct gggctgacag taggtacatt tacttcacct tatatcgaaa
     4381 cgttcaatga acggatcgca atcaatggca aaagcattcc agacaatgat ttgatccagc
     4441 ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca atttgaagca gtagctggta
     4501 ttactgaatt cgagacatta acagcaatgg catttgatta ttttttgatg aagaaagtgg
     4561 acgtagcgat tatcgaagtt ggcttaggcg gcttattgga ttctaccaat gtagctgtac
     4621 caatgctgac gggaataaca acgattggac ttgatcatac cgaaatactt ggagatacaa
     4681 ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca gtagtaacag
     4741 gaaatattgc cccagaagct tttactgtca tccaacaaac agctaaagca aaaaatgcac
     4801 cgctttatgt atacggggaa aattataaaa taaactatct gcatcctgat gagatgtggg
     4861 gagaagtatt tgaatttttt aatgaaatag gaaagatccc tcaattaacc actccgcttt
     4921 tagggcaaca tcaagctgaa aatgcaggag tagccatcga attgtatttt gtttactgtc
     4981 aattggaaaa gctgcctttc cgagaaaaag atgttttgaa gggattgaag aaatctcgat
     5041 ggccaggaag aatggagcgt ttgagtaacg agccattagt tgttttagat ggtgcccata
     5101 atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa ggatatcaaa
     5161 tcaacatttt gttttccgcc ttacgaacta aaaatgttag agaaatgcta gaacgattgc
     5221 agcaagtacc aaatgtccat atttatttga cgacctttga acatccaaaa gccatcgaat
     5281 taagtgaagt atcagattta gcaagtgaga gagtatcaat cgtttcattg tggcaattcg
     5341 gattagcaga tatattggaa aagatgacaa atgaagacgt attagttgtg accggttcac
     5401 tttatttcat ttcagaagtt cgccatttat taatacaatt aggaggagat catgaagtat
     5461 aatgcagtga tttttgacat ggatggtttg ctgttcgaca cagagttagt gtattacgaa
     5521 gccacacaag tagtagcaga caagatgggt tttccttatg ataaagacct gtatttaaaa
     5581 ttcttaggtg tttcagatga agaagtatgg gcgaattatc atatgatttt ctcggaattt
     5641 ggcaaaacaa gtgtacaaag attcatagat gattcatacc aggaaacgct tgaacgtttt
     5701 tcgcaaggag cggtacaaat gaagcccggt gtgttggaac tcctctcttt tttagaagaa
     5761 catcagattc ccaaagttgt cgcttccagt aaccaacgag cagtcattga acttttattg
     5821 aagaagaaca atctgtttga acgatttgag acgattgttt ctgcagaaaa tgttcaacga
     5881 gcaaaaccag acccagaaat tttcttgtta gctaatcaga agctaggtac agcgaagaaa
     5941 gaaacattga ttttagaaga ttcacaaaat ggtattttag cagcttctgc ggctgagatc
     6001 ccggtagtca tggtaccaga tctgcttcca ccatcccaag aattgatcaa aaaaacagct
     6061 ggggttgttt cttctttaca tgaaatccct gcattgttaa aataaaaatc catctctttt
     6121 cgtatcttat tttagataag atagaaagga gatgtttttt tgggaaaacg attgatcaaa
     6181 gaagtaccta ctagctcttt accaagggaa cgtatggaaa tttatggcgc ggaagcttta
     6241 tcggatcagg agcttttggc aatcttactg cgtaccggtc aacaccctta tagtgtgatg
     6301 tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg cctctttacg acaggctact
     6361 ttgcatgagt tagaagagat tcgagggata ggacgcgtga aagcaatcga aatcaaagcc
     6421 ttgatcgaat taggtcgacg aattcagacg gatcatgaaa gagcggctcc accggttcgt
     6481 accagctacg aattggccca gcaattgatt ctggaaatga aagatcacaa acaagaacat
     6541 ttggtttgta tttatttgga cacgaagaat caagtgatct taaaaaaaac agtgttcatc
     6601 ggttcgctga atcagtcagt cgctcatcct agagaaattt tccattatgc tgtacgttat
     6661 tgtgcagcaa ggatcgtttt agcgcataat catccttctg gtaatgttat tccatcgcag
     6721 caggatatga attttaccaa aaggatacaa aagtgtggag aaatgatggg gatcactgta
     6781 ctggaccact tgatcatcgg acgcaaacga tattttagtt tgagagaaga aggaatgatg
     6841 gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca agtgtacaat tatattgtaa
     6901 ttttgtttgg gaatagaagt tgaaacatct gttgcacctc tatcgtacac gagcaatgtt
     6961 acaatttaaa ttagtgcttt tggcacgagg aggatattat tatggaaaac ggaacagtaa
     7021 aatggttcaa cgctgaaaaa ggatttggat ttatctcacg tgaagatgga agcgacgtat
     7081 tcgttcactt ctcagctatc caaggtgacg gattcaaaac attagaagaa ggtcaagcag
     7141 tgactttcga cgttgaagaa tcagaccgcg gcccacaagc tgcaaacgtt gtaaaagctt
     7201 aattttatct gtaaatcaaa ttgatggtag acatcaattt ttacgagaga acaaagcgag
     7261 taacaaaaat acctgccaca agagacaatt tctcttgtgg caggtatttt tttgtatgaa
     7321 aaagtaaata aatttaagag gctatgaaag agccaccttg tatcaaggaa taagaacaaa
     7381 caaaaatggc ttctcatgtt tttcatttgt tttctcttat attcaggaca aggtgctgat
     7441 ttcataacct cttatttatt cttcatctgg tgtcattacc attggcaaaa tcattggatg
     7501 tctttctgta cgttcgaata agaaaggctg aagcgcgcca gtcatcgctt ctttcaattt
     7561 atattctgaa cagtcaggtc cattcaaaca ttctcttaaa gcgtggaata agattcgctg
     7621 agcttcattg atcatttctc ctgattctcg catgtagaca aatccacgag agagaatatc
     7681 tggaccagcc aagatttctt ttttcttgat atccactgtc gcgacagcta atacaagacc
     7741 ttcttctgaa agcaggtgac ggtcacgtaa aacgacatta ccaatatcac cgatcccgct
     7801 accatcgata tacacatctc cagccacaaa atgcccagca ggacgcgcag aatcgctagt
     7861 taacgctaaa acatcgccat tttccatgat gaagcagttt tctgcaggaa cacctacatc
     7921 ttgtgctaga gaagcatgta ctttcaacat gcggaattct ccgtggacag gaataaaata
     7981 ttttggcttc atcaaacgaa gcattaattt ttgttcttct tgtccaccgt gaccggaagt
     8041 atggatgtta ttgatttttc cgtgaatcac ttctgcacca gcttcagaca gcaaattgat
     8101 caaatgattg acacttgttg tattaccagg aataggtgaa cttgaaaaga tcactgtatc
     8161 gtctggctgg atttggattt gacggtgagt accattcgcg atgcggctca acgcggccat
     8221 cgcttctcct tgagaacctg tacataggat catcgtttca ttggcaggca agcgattgat
     8281 ttcgtgtgca tctacaaatg tgccgtctgg aactttgata tatcctaaac gttgaccgtt
     8341 gacgatggca ttttccatgc ttcgtccgaa aacagcgatt ttacgaccag ttgccacagc
     8401 ggcatctgct gcttgctgca aacggaagat gttagaagca aagctggcaa aaatgatacg
     8461 tccttcgatt tttgaaaaga tcttcaaaat agaatgccca atcgtttttt ctgatttagt
     8521 aaatgttgga atctcagcat ttgtactatc agataaaagg cacaagactc cttcttcccc
     8581 gatacgagcc attttatgca agtttgctgg ttcgccaaca ggagtgaaat cgaatttgaa
     8641 gtctccagtc gctacgatat ttccagaagg tgttttcacc acgatcccta atgcatctgg
     8701 gatactatgg gtcgttctga aaaagctgac agaagtcttt ctaaaacgga tgatcgtatc
     8761 ttcattgatc tcatggagct ctgcatctcg aagcaagcca tgctcatcta atttgtttgt
     8821 gatcaacgct aatgcgagag gaccagcata gataggaata tttgcttgtt tcagcaagta
     8881 gggtattccg ccgatgtggt cttcgtgccc gtgagtaatc accaaggctt ttactttgtg
     8941 taagttttgt atgatgtagc tgtagtcggg aatgacatag tcaattccaa gtaaatcgtc
     9001 ttccgggaat ttgatcccgg catcaatcaa aatgatttca tcttggaatt gtaccccata
     9061 cgtatttttc ccgatttcac ctaatccacc aatagcaaag acgcctgtct cgttattttt
     9121 gatggaaagt ttcatattaa aactccacta atgagaaatc agcattttct ttttcgtact
     9181 ctagatgact tccttctaga agttgaacga attcaatatt gtatggggta ttttcttcca
     9241 cacgttggcg agcgtctaca tcgctttttg cctcaatgta aagagatttt gtttcttccc
     9301 gtttagggtt gcgtactttt gtttcttgat aataaacttt atagatcatt gttttgtctc
     9361 ctttatctgc gtaaagcagt gatttagcta gttgattttt ttagacaaca caaaacgagg
     9421 ggacaagcca cctggttttt catatgcttt gactagaata ctggattcga gtcaaagaga
     9481 caagcctcgt ttttgcttat ctgttcggat attttttcaa ccgtaccttc tgtataaaaa
     9541 atatacaaca attaattgtt attctagcat aaaacagcaa ataagtatag aaatcaacta
     9601 gattgaaaga aaaaatcaat gtttatcatc ccgctataat cataatgact tttttatgag
     9661 agaagtgttc tattaatagg ctcgttgtgc tatcattttt agtaatgaaa atgtaacaaa
     9721 agattaaaag gagatgagaa agtgaaacta atgtggcatt ataccatgcg ttataagaaa
     9781 ttcttgtttt tcaatttcat atgcgtattt ggttttattt tgattgaatt aggacttcct
     9841 acgattttag cacgtatgat cgatgtagga atccgcaatg atgattttga ttatgtgaag
     9901 caacaaggga tatttatgat cgttattact gtaatcggta ttattttgaa tatcttgtta
     9961 ggttattttg gttcgaggat tacgactaat atcgtagcag atattcgaga tgatttgttc
    10021 gaaaagatcc aaagttattc gcatcaggaa tacgaaaatc taggcgtatc ttcattgatc
    10081 actcgtacaa caaacgatgc ttatcagatc atgttgttcc tgcaaaatat tttacgtatc
    10141 ggttttatgg caccaatgat gttcattgtc agtttgtaca tggtcatgcg tacaagtgtg
    10201 acactcagtt tgtatgtagt aggtgcatta cctttactat tattggcagt cgtagcgatt
    10261 gctaaggtgt ctgaaccgtt atctaaaaag caacaaaaaa acttagataa aatcaacagt
    10321 atcttgcggg aaaatctttc aggattgcgt gtgattcgtg cattcgtcaa tgaaaagttt
    10381 gaagaaaaac gttttgctgt agtcaatgag aattatgctt ctagttcaaa aagcttattc
    10441 cgtttgatgg ctgttgcaca accgggtttt tatttcttat ttaatatcgt aatggtattg
    10501 atcatctgga gcggggcagt gcaaatcgat gcaggcgagc ttcgtgtagg agatttgatt
    10561 gcgtttatcg agtatatatt ccatgccttg ttctcattca tgttatttgc tagtgtgttc
    10621 atgatgtatc caagagcagc tgtttcagct ggacgtatcc aagaagccct agatgccaaa
    10681 ccttcgattg tagaagatcc aaatggtata tctgatacaa aaactaaagg gtatatcgaa
    10741 tttcaaaatg taacatttgc ttatccaggc cattcgcaag agccagtgat acgaaatgtc
    10801 agttttacag cctctccagg agagaccgtt gcttttattg gaagtaccgg tagcggaaaa
    10861 tctacgttga tccaattgat ccctcgcttt tatgatgtga gtgaaggaaa gatcttgatc
    10921 gatggagtag acgtgagaga ttatcggcta agcaagttgc gagataagat tgggtttatt
    10981 ccgcaaaaag cgctgctatt tactggaacg attgcagata atttgcgata cgggaaagaa
    11041 gatgctacgc aagaagaaat ggaacgtgca gctgagattg ctcaagcttc agaattcatt
    11101 tctcgaaaac cagatggatt taatgaacat ttatcagaag gtggttccaa tttttcaggt
    11161 ggacaaaaac aacgattggc aatagccaga gccatcatac gccgtccgga aatctatatt
    11221 tttgatgaca gcttttctgc attagattat caaacggatg cgaagttacg tgctcgtctg
    11281 aaaaaagaaa cgacagaatc tactgtattg atcgtagcac aacgtgtggg aacgatcatg
    11341 catgcagata agatcatcgt attaaatgaa ggagaagtcg tcggtatggg gacgcatcgg
    11401 gagttgttgg aaaaatgccc gatttattat gatattgccg cttctcaatt atcaaaggag
    11461 gaattagcat gaaaaaaacc ttttcttcct taaaacgttt agctcgatat atcagaccgt
    11521 atcgtgagac ttttctttta gtgatcctat ttacagttct gacagtggct ttcaacgtgg
    11581 ctttgccata tgttacagga cttcctacta cagaaatcag ccgtaatatt gccaatggcg
    11641 aatcaatcaa tttttcttat atattcaaat gtctcatttg gatcacagtt gttggaatag
    11701 ggtattgtat ttcgcaatta ctttctggtg ttttgatgac aaatgtcgtt caaagtgcca
    11761 tgcatgattt gagaagagac atagaagaaa aaatcaatcg tcttccggtt tcatatttcg
    11821 ataaaaacca gcaaggaaat attttgtctc gtgtgacgaa tgatgtagat gcagtcagcg
    11881 gtgctttaca acaagctttt attggtatcg taaatgcgat attgggaatt acgttggctg
    11941 cagcaatgat gttttatatc cagccgatga tggcgctgat ctctatgatc atgattccaa
    12001 catcgatctg gatctcaaaa cgagtgatca atgcttcgca gaaatatttt caaagcatgc
    12061 agaattctct tggtgagcta aacggctacg tccaagaaaa tatgacaggt tttagtgtat
    12121 tgaaagtata tggacgcgaa aaagaaacat tggaaggatt caaaaaagtt aatcacagct
    12181 tgaaatactt tgggtttcgt gcagcattca tttctggctt gatgatgcct ctagttcaat
    12241 taactgccta tgctacttat attggtatgg ctgttctagg aagcttttat gttattactg
    12301 gggcgatcgt tgtcggtcaa ctacaagcat tcatccaata tatttggcaa atcagtcagc
    12361 caatggggaa cgtgacacaa ttgtcttcag ttttgcaaag tgcatcagcg gcaacgaaac
    12421 gtgtattcga gattttagat gagccagaag aaaaagaaaa caaagtagat gcttcactgc
    12481 cagaaacgat tttaggagat gtgacattcg atcatgtcga ttttgcatat gatccgaaga
    12541 aaccattaat ccaagatttg aattttgaag tgaaagctgg tcaaaccgta gctgtggtcg
    12601 gtccgaccgg tgccggcaag acgacattga tcaatctatt gatgcgtttt tatgacgtag
    12661 acaaaggagc aataaggatt gatggaatcg acaccaaatc aatgagtcgc agtgatgtgc
    12721 gttcgctatt tggtatggtc ttgcaagatg catggcttta tcaaggaacg attgcagata
    12781 atatccgctt cggaaagctt gatgcgacag attatgaagt agttgatgct gcgaaaacag
    12841 caaacgtaga tcattttatc cgaacgatgc cagatggata tgatatggcc atcaattctg
    12901 agggagacaa tgtgtcttta ggacaaaaac agttgctgac gattgcccga gcagtggtct
    12961 ccgatcctaa aatcttgatt ttagatgaag caacaagttc agtagacacc cgtttagaag
    13021 cgttgattca aaaagcaatg gatcgcgtga tggagggacg gacaagtttc gtcatcgctc
    13081 accgtttatc gacaatccgt gatgcagatc tgattctagt gatggatcaa ggtcagatca
    13141 ttgaaaaagg aacgcatgag agtttattgg cacaaggtgg attttatgaa aaactgtata
    13201 acagccaatt tgctgaggaa gcagaataag tcatataaga ataaaacagt ttaaaactta
    13261 ttttttgttc tctattcttg ataatggtaa agtaaagtta tcaagaaaag tgaggacacg
    13321 gagatggaac agtttttaaa tgaaaaaatt actgaagcca aaagagaagt tgatcgttta
    13381 acgcacgtct gtcagacgaa gccttttgat tcagctgatt tttttgaaaa tgagtttcaa
    13441 atcgaacgct tggcagcaca aatagaagtg tacgaattaa ttttaaaaaa agtaatagaa
    13501 gaaaatatag atgtacaata aaaacaggta aaacgtcagt taccagatac tgtaaataga
    13561 catctggcct gagcaggttt tacctgtttt ttttcttatg aaataaatat atgctatgat
    13621 acctttattc aataggatta gtttctacaa aaaatgaaag gagaaaaata aatatggaac
    13681 ggtttatagc atcaaatgcc acagtgattg gtgatgtgac attgtcagaa gatgtgacga
    13741 tctggtatca agctgtttta cgcggggaca gtaattggat aaaaatagga caaagaacga
    13801 atatccaaga tggtacaatc atccatgtcg atcatgatgc acctgtagat attgcggaga
    13861 atgtgacggt aggacatcag tgcatgctac atggctgtac aatcgaaaaa ggggcgctga
    13921 tcgggatggg gaccacgata ttgaatcatg cggttattgg tgagaatagt ttgattggtg
    13981 ctggatcatt agtaacagaa ggaaaagtta ttcctcctaa tgtcttggct tttggtcgcc
    14041 cagctaaagt gatccgtcct ttgacaaaag aagaaataca aaaaaacaaa gaaaacatcc
    14101 agcattatgt cgagataggt cggatgcatg cgcaaggaaa attcaaggag caaagatagg
    14161 cagtagcatc cataaaataa aaaaggatgg tcagtttgag ttgaccatcc ttttttattt
    14221 ggtttagata agagagctta aaaaagcttc ctttgaaaca gattaattta atgaagaact
    14281 gttttccatg acttcatcga tcaatccata tgcttttgct tgttcagctg tcatatagtt
    14341 gtcacgatct gtatcttttt caatcacttc aagcggttgt ccagtgcgtt cagctaaaat
    14401 tttgtttaag cgttcgcgtg tttgcaaaat atggcgtgcc gcaatttcga tttcagttgc
    14461 ttgcccttga gcgccgccaa gaggttgatg gatcatgatc tcagcatttg gtaaagcaaa
    14521 tcgtttacct tttgtaccag ctgtcagcaa gaagcttccc attgaagcag ccatacccat
    14581 aacaattgtt tggacatctg ctttgacaaa gttcattgtg tcgtaaatag ccatacctgc
    14641 tgtaactgaa ccaccaggag aattgatata aagatagata tctttttcag aatcttgagc
    14701 gtctaagaaa agcaattggg cgatgatcga attagcaaga tcatctgtta cttggccgct
    14761 taacatgatg atacggtctt tcaataaacg tgagtagata tcgtaagctc tttcacctcg
    14821 tgaagattgt tcaatgactg taggaattaa attcataata gagttcctcc ttgatttcgt
    14881 ttagaaatag tgtaccataa tttagtttct aatacctaaa atgtagtata catttttggt
    14941 caaaaaaggt caaataaaaa acttaggcaa attagtcgca taaacccgct ttatggtatg
    15001 ataagcagag aaacaaaaca aggaggaaca acagattgat agttggtatt actggcctta
    15061 tcggagtgct gacaggtgct gtagttgtct atatcggcat gatactgcaa atgcgtcact
    15121 tggaaaataa agaacagcgt agacgtgaat tggaattgaa agtcaaagaa atcgagacgc
    15181 tgaatcaatt aaataaaaag atcaacgaga tcttgcaaaa acggacaacg ctgatggaag
    15241 aatatgtcag ctttgatgca tttgatgatt gttatatcac gattgacgat tttgcgtatc
    15301 tccaatcatt ttcagcgcaa aatagttttt atctgccaaa ttatatttta gacgagctat
    15361 ttaagaatat ctcccatcga aaagtgattt tatctccaga tgaaacaatg aagattggtg
    15421 gatatgctta taaaggcgga cgcgtgattt tagagaattt tacggatagt ctgactgaaa
    15481 tgatcaatga aaaaaaagct cagttgaaga ctatgacgcg ccagccgatt ggtttcttct
    15541 cgaaagatga tgtttggagc tgatattttg aagcattgcc gttgcacatg agagcgtggt
    15601 aatgctttct ttttttctta atgatacaat agaactgaac ggagtgaaga aagatgttac
    15661 aaatctatgc ccatcgcgga agcagcggca cccacccaga aaatacgctt cctgcatttg
    15721 cagaagctgt acgagtaggt gcagatggta tcgaactaga tgtccatcta agcaaagatg
    15781 gttatttgat cgttatgcac gacgaagaag tggaccggac gactaacggt aaagggctga
    15841 ttcgagaaaa aacattggaa gaactcaaaa aattgaatgc agggggcaaa ttcagcaaag
    15901 aatttccagc agctaaagtt cctgaattga gagaagtgtt aggactgctc ctccaaaaaa
    15961 attaccgagg gatgctaatg attgaaatta aaactgatca gtatgaatat cccggtatcg
    16021 aagaaaaatt agccgaaaca ttaaaaagta gagattggcc ttttcaacat gcatattgca
    16081 gctttcattt ggattctttg gaaaaaatcg cattactgga accggagata cagctggact
    16141 tattgatgag tacttctgat aaaaagatcc aactagcagc agaaacccct tatatagaag
    16201 gagtacatcc aaaaaatact tggttgttcg aacatccagt ccaggcagct gattttccaa
    16261 aagcaattcg tgcatggacg attaacgatg aagaagaaat ggccaaatgt caagaactca
    16321 aaatagatgg aataataact gattttccgg agaaagctct tcggtggaaa caatcaacga
    16381 aaagtaggta aaatatggaa aaagtattag tgatcgtcgg tccgacagca gtcggtaaaa
    16441 cagcgctaag tgtggaactc gcacaactat ttggaggaga gatcatcagt ggtgattcat
    16501 tgcaaatcta taaaaatctg gatatcggta cagcaaaaat caaagaagaa gagatgaata
    16561 atgttccgca tcatttgatt gatgtgatcg aacctactca acgttactca gccgctgatt
    16621 ttcagcattc aggtcgacaa ttgattaccg agattgcgaa ccgagggcac ctacctatca
    16681 tagctggtgg tacgggtctc tatatccaat cccttctata tgattatcag ttaggagcga
    16741 cagaagaagc gaatcctgag attagaaaaa agtatgaaac gttagctgaa acaattggtt
    16801 cccaagcatt atggaatcag ttgaaagaaa aagacccggc agctgctgag aaaatccact
    16861 ggaacaatca acgcaaagtg ataagagctc tggaagtttt agagacgaca ggacatagta
    16921 tcacggcacc taaagaagag ccaaagcaat tgtatgatta cctcatgatt ggattagata
    16981 cgaagcgtag tattctttat gaacgaatca atcaaagagt cgatttgatg ctagcagaag
    17041 gtttagtagc agaagcgcgt aaggtgtatg agcttggaga agtccaggcc agtcaaggta
    17101 ttggatataa agaatttttc ccttattttg aagggaaaga aacattggaa gaagcaacag
    17161 aacaagtcaa actacattcg agaagatacg cgaaacgtca gttgacgtgg tttcgtaacc
    17221 gtttggatgc acagtggttt gatcttataa gtgagccaaa tcaaaaaagt cagattgagc
    17281 aaacaatcaa aaaatggctg gaggcggaat aaaatggaag aaaaagtgat catcgtaggg
    17341 gtagaaaccg aagcaaacca gcgttacttt tcagagtcaa tggaagagct agttcagctg
    17401 acgaataccg catcaggaga agtcgtattc acaattacac aaaagagacc acaagtggat
    17461 cggcaaacga ttatcggtaa agggaaatta caagaattag ttcaacaagc ggatgcgcat
    17521 gaagcggatt tgatcatctt taatcatgag ctaactccga gacagagcca actgattacc
    17581 gatgctgttg gtcttccggt aattgatcgc gtgcagctta tcttagatat ttttgcgatg
    17641 cgtgctcgtt cgaaagaagg gaagctgcag gttgaacttg ctcagttaga atacctgctg
    17701 cctcgtttag tagggcaagg gaaaacatta tctcgtcttg gcggtgggat cggaacaaga
    17761 gggccaggtg aaacaaagtt agaaacagac cgccgacata tacggaataa aatttttgct
    17821 gttaagaaag aattgaaaga agtagaagct catcgagagc gcaatcgcca aaaaagaaaa
    17881 aatagcgaga tttttcagat tggacttatt ggctacacga atgccgggaa gtcaactatc
    17941 ttgaatctat tgacacaagc ggatacttat tcgaaagatc aattgtttgc aacgttagat
    18001 ccattgacta agagatggcg ttttgctgaa ggatttgaga ttactgtaac ggataccgtc
    18061 gggttcatcc aagacctgcc tacgcaactg atcgatgctt tccattctac attagaagaa
    18121 agtcaaaata tggatctttt gctacatgta gtcgatgcta gttctccaga tcgtatttta
    18181 caggagcaga ctgtcttaaa attgatggat gaactgaata tgaaagaaat gccgatactg
    18241 actgtttaca ataaggcaga ccagatcgac ccagcgatgt tcacgcctac gctattccca
    18301 aatgtattga tttctactca gacgaaagaa ggtaagatgg cgttgatcga agcaatcaaa
    18361 cgtcagctga tggaactaat gaccccttat acactttctg tgccaagcaa cgaaggacag
    18421 aaattaagcg agttgagaag acaaacaatg gttttggacg aagcgtacat ggaagaaaca
    18481 aacgaatatg aagttaaagg gtttgcaaaa accaattcaa aatggttaag aaaaaaccaa
    18541 taaaatttta tttttttaa
//
LOCUS       DAYYCQ010000057        17888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000057 DAYYCQ010000000
VERSION     DAYYCQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.057"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            474..725
                     /locus_tag="ACHAYF_001916"
     CDS             474..725
                     /locus_tag="ACHAYF_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579353.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="ACHAYF_001917"
     CDS             759..1037
                     /locus_tag="ACHAYF_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579354.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="ACHAYF_001918"
     CDS             1634..1891
                     /locus_tag="ACHAYF_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579355.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="ACHAYF_001919"
     CDS             2004..2615
                     /locus_tag="ACHAYF_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579356.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="ACHAYF_001920"
     CDS             2794..3351
                     /locus_tag="ACHAYF_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579357.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="ACHAYF_001921"
     CDS             complement(4050..4571)
                     /locus_tag="ACHAYF_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8579358.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="ACHAYF_001922"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="ACHAYF_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8579359.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /gene="cdaA"
                     /locus_tag="ACHAYF_001923"
     CDS             8651..9538
                     /gene="cdaA"
                     /locus_tag="ACHAYF_001923"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8579360.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="ACHAYF_001924"
     CDS             9535..10704
                     /locus_tag="ACHAYF_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8579361.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /gene="glmM"
                     /locus_tag="ACHAYF_001925"
     CDS             10728..12083
                     /gene="glmM"
                     /locus_tag="ACHAYF_001925"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8579362.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="ACHAYF_001926"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="ACHAYF_001926"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8579363.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14369..14659
                     /locus_tag="ACHAYF_001927"
     CDS             14369..14659
                     /locus_tag="ACHAYF_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579364.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14672..15697
                     /gene="sppA"
                     /locus_tag="ACHAYF_001928"
     CDS             14672..15697
                     /gene="sppA"
                     /locus_tag="ACHAYF_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8579365.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15700..16359
                     /locus_tag="ACHAYF_001929"
     CDS             15700..16359
                     /locus_tag="ACHAYF_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8579366.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(16483..17784)
                     /locus_tag="ACHAYF_001930"
     CDS             complement(16483..17784)
                     /locus_tag="ACHAYF_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8579367.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         5777 a         3096 c         3861 g         5154 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccga
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc accacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagcactt
    12901 ggcggctgtc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacagga atctgtgtca
    13021 atttttccaa agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagcatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccgttgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccatcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 cattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctccttttt gttttttatc tggaaacatt taaaatcata accttttgcg aatgttcttg
    14221 tcaacaaaaa aagaataaaa aataaattgg tatatatcaa attctttgtc aaatgtcctt
    14281 tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc ggtacaatag
    14341 aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca tgacactggg
    14401 aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag atgatacgaa
    14461 aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg ccttggaagt
    14521 tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt tttccgggta
    14581 tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa ctgtgatcgt
    14641 cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg cagcagttct
    14701 gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta agcctcagga
    14761 aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac tagctccaaa
    14821 tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat
    14881 tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata aaaattttat
    14941 ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa
    15001 ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg atacgattcg
    15061 taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta gcggtggcta
    15121 ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga ccggctcaat
    15181 tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg gtgtggaaga
    15241 tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac cagagacaaa
    15301 agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt ttgtagcaat
    15361 cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg atggaaggat
    15421 ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat ttcctgaaga
    15481 tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag tagaatattc
    15541 ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag aattccaggg
    15601 attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag ggacagcgga
    15661 atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct ttcaaatgaa
    15721 aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga ctccaacttt
    15781 tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag
    15841 aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa agcaaatgat
    15901 tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt ggtcgatctt
    15961 ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct aggttggctt
    16021 aatttttcag attcttatct gagtctctac ggattcacag ccttacttat ttatctagcg
    16081 tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat gatctttggt
    16141 attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt agttagagaa
    16201 ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct tccagcagtc
    16261 tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt tgttacgatc
    16321 aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa aagcagtaac
    16381 agttgaaagc accttctaaa agtcagatct aaaatcggct ctttgtcaaa taggactgat
    16441 agagtgagat aagaacatct aaggtagaat tccttttcta ctttagatgt tttttgattc
    16501 ataggtaatc agattattta gtaagaaaat tctggtcttc attttccgaa agcttcgaaa
    16561 gccataagcg actcgtttca atgctttgat gtgggtatgc agattttcta ttttggcatt
    16621 tgaataaggc aattcgaggg ctaaacgaat gccggattca tagtttataa aagctttttt
    16681 tatagaatgc agttcttttg gaacggaatt gggcatcgtt cttacaagtt taaagaaagt
    16741 gtccgcctct ttgtcgtgaa acgcttgaag aatttcttga tagtaaaaat aggcgattcg
    16801 taattcgtca gagatcgaaa gcagacgatc caccacttca ctttcggtga gatacgccca
    16861 accaaaattg cgccatttgg tccatttgtc ataaacaagc ttgtcctgtc gtttgatcag
    16921 aagccgagac aacgccttta attgtcgata atatttggct tgtgttggat cgtttcgatc
    16981 aaagcttttc atgattcgta cgcgaatatc ctcaaattta cgaatgagat gtttgacaat
    17041 gtgaaaacga tccacaacga gttttgcctt tggaaaacat tcctttatca gagaggcata
    17101 agaaacattc atgtccgtca cgatgacctt taccttcaat cgtgcgcgtc gttcaaattg
    17161 cataaaataa gaaacgagag cctttttctt ccgtgaagga agaatatcca gtaattttcc
    17221 agtatcgcca tccatacaag aaaaggagta gggaccgact tgatttttca gtgaacgaaa
    17281 ttcatccatt aataaacaac gcggtaacca atttcttttt acttttgtca tggggatcag
    17341 agaagcgagt gtccgagaaa cggtcgacca agaaacgccg caatgcttag caattgtcgt
    17401 cattgcttgt ttttcagtta gctcttctaa aattttatag cgcacttgtt tagcgataaa
    17461 gcagtttcta tcgacaatgt aggtattcgc cgtaaaatag gtgtggcagc cacgacagtg
    17521 gtagcgttgt ttcgctaaag caagataagt cagttggttc gcacatttat taagttgaat
    17581 gagcgatttt ttctttccgt tcttaacgat catgccttca tgattcatct gtttacacaa
    17641 aggacagcga cgaatttctt tacttaacgt cgcagaaaca acaagactct tgatattctt
    17701 aatgctcttc gattccacat ttgtgatgaa aatgttttta tctttgattc caagtagtag
    17761 tttgatatga tgaagatcag gcatacagaa cgctcctttg attgtgttgt ggtgattcaa
    17821 ttctaacgga gcttctgtat gttttgtttt ttcatgctca aaagggaccg atagaatgat
    17881 tttctatc
//
LOCUS       DAYYCQ010000058        17411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000058 DAYYCQ010000000
VERSION     DAYYCQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.058"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..536
                     /locus_tag="ACHAYF_001931"
     CDS             99..536
                     /locus_tag="ACHAYF_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579368.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            631..1515
                     /locus_tag="ACHAYF_001932"
     CDS             631..1515
                     /locus_tag="ACHAYF_001932"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8579369.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1739..4906
                     /locus_tag="ACHAYF_001933"
     CDS             1739..4906
                     /locus_tag="ACHAYF_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8579370.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4974..5471
                     /locus_tag="ACHAYF_001934"
     CDS             4974..5471
                     /locus_tag="ACHAYF_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8579371.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5490..5954
                     /locus_tag="ACHAYF_001935"
                     /pseudo
     CDS             5490..5954
                     /locus_tag="ACHAYF_001935"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5951..6709
                     /locus_tag="ACHAYF_001936"
     CDS             5951..6709
                     /locus_tag="ACHAYF_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8579372.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6795..7535)
                     /locus_tag="ACHAYF_001937"
     CDS             complement(6795..7535)
                     /locus_tag="ACHAYF_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8579373.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7596..8849
                     /locus_tag="ACHAYF_001938"
     CDS             7596..8849
                     /locus_tag="ACHAYF_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8579374.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9171..9398
                     /locus_tag="ACHAYF_001939"
     CDS             9171..9398
                     /locus_tag="ACHAYF_001939"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8579375.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9521..9847
                     /locus_tag="ACHAYF_001940"
     CDS             9521..9847
                     /locus_tag="ACHAYF_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8579376.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9915..10628
                     /locus_tag="ACHAYF_001941"
     CDS             9915..10628
                     /locus_tag="ACHAYF_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8579377.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10938..11788
                     /gene="licT"
                     /locus_tag="ACHAYF_001942"
                     /pseudo
     CDS             10938..11788
                     /gene="licT"
                     /locus_tag="ACHAYF_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            11909..13774
                     /locus_tag="ACHAYF_001943"
     CDS             11909..13774
                     /locus_tag="ACHAYF_001943"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8579378.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13788..15231
                     /locus_tag="ACHAYF_001944"
                     /pseudo
     CDS             13788..15231
                     /locus_tag="ACHAYF_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15339..16040
                     /locus_tag="ACHAYF_001945"
     CDS             15339..16040
                     /locus_tag="ACHAYF_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8579379.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16166..16678
                     /locus_tag="ACHAYF_001946"
                     /pseudo
     CDS             16166..16678
                     /locus_tag="ACHAYF_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(17076..17285)
                     /locus_tag="ACHAYF_001947"
     CDS             complement(17076..17285)
                     /locus_tag="ACHAYF_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8579380.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5816 a         2920 c         3731 g         4944 t
ORIGIN      
        1 aaaatttttt ttacttagaa tccagcgacc ctagatattt ttctgtataa tcttaaataa
       61 agaaaacaat atcaaaaaat gcacgtagaa gtagataaat ggagtggttt tgcaaatttc
      121 ttttatttct agaagttatt cacgtatacg gatttgtttt tctcacaaat aaaaaggcag
      181 gaagtacaat ggatcatttg tatataaaat atattcatca attttccgtt ttggtgttag
      241 gcacactaat tatagcgggg attgtcgcat ttattattca aggccgtgac atattaacag
      301 tacatcaaga gtttttcagt tatgcatttg gtattcctct gatcgctata aggtctgata
      361 acaaaaagtg gaagaaacgc tattacattc tcttgctatt tttgtgtttt tatctgccac
      421 ctaagatttt tactttatta ggtatacaat gcctatcatt ttcttttatt tcctatatca
      481 ttgttttaat tttcctcagt ttcatagtat ggatgtttta tcttgtcaaa aaataaatga
      541 atgtttcatg aacagtactt catcacagcc taattttttt ctttatgcta tcgttatatt
      601 aaagaaatca atcagaaggg aaagaaagtg atgaaaaaag ttttattagt ggtaaacccc
      661 agttcaggcg gtgaacaagc aaaggaattt gagcaattag cgatagcaaa attggaatcg
      721 gtgtttgatg aagtggtcgt cttgcataca aaaaaagcag gggatgcaaa aaactttact
      781 cgcgaagcgg ctacggaagg gtatcatagt gtgtttgtca tgggcggaga tggaacagtc
      841 aacgaaggaa tcagtggaat cgctgaacaa gaacatcggc caaatttcgg atttttccca
      901 ttgggtacgg tgaatgactt ggctcgagca ttagggatac cgctagaacc tgaggaagcg
      961 atcaatcatt tttcgattga atcagtcaag ccattggata ttggaaaaat aaatgacgac
     1021 tattttatga atgtcgtcgc aattggctct atccctgaat caatcaatga tgtagatgcg
     1081 gagaagaaga cgaaatttgg aaaactagct tatttcatgt caggaatcaa acagttagct
     1141 tccacacaat cctactcgtt tcacgtagaa gttgatggaa agaaggaaga aattgagagc
     1201 agtaccttgc tgataggact aactaactca gtcggcggtt ttgaaacatt acttccaaat
     1261 gcaaaagtgg atgatggtaa gctccatttg gtttatttaa aagattcttc tttgatcgat
     1321 acgcttaaaa cattgccaga cctattaaaa ggagtagatg aatctacgaa aaatctagcc
     1381 taccttactt gtgagcaatt agttgtgtca ttagctgatg aggaagaatt gacgacgaat
     1441 gttgatggag atgaagggga taaattacct gtgacaatca aagtattgcc ctcacattta
     1501 caggtttatt gttaaaatgg aggttgtgac cgaggagttg gcgggagaac accgcttgtt
     1561 cgggataagg gactgtgaca agacttttgt cacagccttt tttttgtaca aaaaagaaat
     1621 ttacacctaa tagaaaaatt tttccataaa aatacatatt ttaaatatga aagtacaatt
     1681 ggaaatagat taaaacgata aaataaaatt gtcaaaacgt cagagaggat ggtggagaat
     1741 gaatcaatgt gtaaaaaagc tgattgcgtc gattttgttt gtcttccctc tcttgatggt
     1801 gacatccgtt tttgccgatg tagagaaaac aaacgaatca acgcaatcag agaatcaaag
     1861 ttttgacaag actacttttt cagaagaatc gattacttct gaaactacag aatctcaaag
     1921 tgaggcttct actaataaaa ggcaagaaga aaataagaca agtgagacct cagaagtagc
     1981 tgaacaaacg tctgatagca gtgaagcggc actggcaaga gctgtttcag cggatgggta
     2041 cagcgaagct gccacaacag aagaactggc gcagcttctc gcagacgaat cagtaactaa
     2101 gatccgactg attcagcctt tgactttaga cagggaatta gagatcaaac gagatatcgt
     2161 tattgatttt ggtggattcg ctcacaattt cggcacacac catatttata tcaatgagat
     2221 acctcagtcg atcgagttcc agaattttaa aggaacagct gcacatccag gtggattgac
     2281 accaaatatc gatggaaatg ccattatcat tgcttatatg accgattttt ggggaagcag
     2341 atatcatttt acgggagaaa taaaatttac cgggacactt gatttgtatg atgggtccaa
     2401 attaggactg atttacgctc ctcgtgcaac tgtcacatta gatggtgttt ccggtgtgtt
     2461 ggatgtccaa ccagtgaagg aaggactgaa tgccgctccg ggtaaagcct atttttgtag
     2521 aagctatcaa ttgaatgtga tcaatggtag tcaactttat ggaccgtatc tagggaaatt
     2581 ctacggtttt ctagattcag gagatgagac tggtagtgga aaaacagcgc caggaatcca
     2641 tatcttaagc ggttcaaagg tctctcttga ttatgatcgg gcagatggcg ttagcgatga
     2701 aggagaggca gtcgatacgc tacctggtaa tgtcatgttt gaagtcagtg gttcgggaag
     2761 cgaattttcg gtaaatacaa agatcaatat taccaatgac aacaatcgcg gaatcatcca
     2821 aatgagaggt tcaggatcga aagtcactgt atcgaatggt gggaaaatca ccatcaatac
     2881 agcaactacg ggcggatttc gcttgcaagg agattctagt caaatccatg tatcaagtgg
     2941 tggacaaatc tcggtcaaaa tggctggaga tgacgatcaa cccgggaaca atgggatgcg
     3001 ttttgtagga aaaggccttt ctttgatcgt agaaggagcc ggatcagtta tcgacatcga
     3061 taagcagagt gggcgttcca gcgcagttcg ttttgagaat ggtacgcaaa agctgacagt
     3121 aaccaatgga ggctcattga gggtaagaaa tacaggtgat ggcaaatctt acgtgaacag
     3181 actcaatcaa ggaaatcaag cgatccaatt ttatgacgct tctggttttc tagaaagtcc
     3241 aggttcagct gattttacag tcactggaga aaaatcaaat atcgatatac gcgcagattc
     3301 cggagcaact gtagatacaa ctggagatat tgatttaaat tttacggcag gtgaaaaaac
     3361 ttatttagtc atggtcggga aaacagggga agataagacc ggcatcatca gtggggatgt
     3421 gttgaatgtg gacttagaat ctcctaccta ttttgatttc caaaacaaac ggattggaag
     3481 cgagaccaat ggcataggcg gctgggtatt ccaaggaaat ggagattctt ctttaaagat
     3541 cgcccattca gaaatcgctt tatggcgaaa aacaggcgca acattcgata actttgaaaa
     3601 agacccggat tatttttctg atatgacaga cttatcgatc aatgggaccg acttaggcaa
     3661 tttcgtatca agccctagtg caggtttaac aaatgaattt gctcagacag gtgccggaat
     3721 gaaaaattat aatcgaatca gtgccaataa tcagctcccg atgattgaat ccttaagagt
     3781 gccaacgaat gcagataaaa aaatctatgg gcatgtcgtt gttcccgaag gtgtcgaggc
     3841 aaagccaaga gatgcttgga caggcgaagt agaagtgact ttacgtatta cttacgcaga
     3901 tacgacgaaa tcatctgtag aaattactgc tctcacgcaa ggacaaacag atgaaaatga
     3961 aggggcagga tataacccat ggggagaagg cgaacaaggc ggcttgttcg aagttcaagt
     4021 acccaatgat gagagattaa aaacggagga tcaagtgcaa gttgttgctg ctaaaaaagt
     4081 aagaggtggc caagaagtga cagaactgga ctctgccaaa acgacagtcg atgtgattcc
     4141 tcctgaacct gctaatttaa gtaccacatt gatcaatgca gcttctcgca cgatcacagg
     4201 tacaggtgaa gcaggagcct tggtttcatt gaaaaaaaat caagctgttt tgtcagaaac
     4261 aactattgat gatacagggg aatttgttct ttctatccca gaaaatcaac tttttgcagg
     4321 agatgtatta gaaattgtat taaacgacca agcaggagaa gctactgcta agggtgtgca
     4381 gaacaagcca agtaccaacg atgaaattgg gaatcacaat ccaagtgata cgccagttgt
     4441 ttatcatgat gccccgcctt ttgcacctgc tacgaagatc gtagtagaag gtggtctctc
     4501 atttcttgcc ccttccaaac tcgattttgg tcagatcaaa gcaactggtt tgacacaaga
     4561 agcatttggc caaatggata tacaggaaaa attatggatt tatgatcagc gagaaacgaa
     4621 aaatccgtgg atcttaacga taaaattatc tcagcctttc acaaatgatg caggattttc
     4681 tatgcaggat gtcctatatt tcaagaaagg agaggagtat caattagtga atgaagaaga
     4741 actggaaatt gtcagaggac aaaattcaac gaatgtgcaa gcagattatt cagaatattt
     4801 aaataacggt cgggcgttta aacttgtctt aaccaaagaa aatcagatag ctggtaatta
     4861 ctcagcagag ataacatgga cattagcaga tgcgccgaca gaataaagat caaggaggat
     4921 gattgatgaa tcgaatatgg aataaaagtc atcgaaacaa ctgctcttta cttatgatcc
     4981 ttctccttgg attcatcctt tggcagacac ctgtccatgc ctccgaattc aattttgcgg
     5041 tagattttga attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga
     5101 tggagcctgg gaaaacacag gaaatcccct acacggtctt taaccgaaca gataaatcag
     5161 taacagttga gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg
     5221 gagaatcaaa aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa
     5281 cagtgggaga aataactgtt cctcctcaaa gtgcggtcac tgagacactt tcactgaaag
     5341 cgccagaaaa agagctggct ggtgtggtcg caggagggat cacgtttcaa gaaaaaaaag
     5401 agaacgtttc taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa
     5461 tcttgatttg aaatagcgaa aaagagtctt ccccagattt gaaattaaaa aaagcttatg
     5521 cggatcaaag cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt
     5581 atatgttcgg cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttacc
     5641 tttcaataaa aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc
     5701 cattagaagg aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga
     5761 aagaaaaacg ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat
     5821 tgaatgaaaa ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga
     5881 tacttgttgg agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa
     5941 ggaaaaagaa atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt
     6001 atgtacacaa agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc
     6061 tgtgaccaaa ccagatgctg caaatcctga agacgttgtt ccagttcctc ctgttgatcc
     6121 aaatgatcct gatactcctt ttacacctga cgaaccaaat cagggaacat ccggattact
     6181 gacgattgac caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac
     6241 agtatatgct gcattacaaa gcggaacgga cagtactgga gcacaaacac aagtacagaa
     6301 tttcgtacaa gtaacggata aaagcggaaa ttatgccggt tggaaattat cagtcaaacg
     6361 aacagaattt attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt
     6421 caaaaacgcc gtgattcgaa atagcgcaac taatagcagt ggaatgccat ctaccgtttc
     6481 tggaactggc gctgaaggaa tcgaaatccc agcagatcag gcagtagatc tagtaactgc
     6541 aggagcaaat gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg
     6601 gagacaatca gtggaattat ttgttcctgt atccaattat gctgtagaca actacgtttc
     6661 aacattcaca tggtcgttaa cagatgctcc tacagaagag gaagcgtaaa atcatatcaa
     6721 cgatttcagg gagtggagcc gattcaaggc ccactccctg aaatctgcta tgcttacgtc
     6781 tcagctctta ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc
     6841 ttttcaaaga tctttcgacc aacgagtaag gagtcaccgt tgaaaaattc taacacataa
     6901 tgagcagaat caattttctg caattgcgat aagttgatac agtaagattt taaaagtaaa
     6961 aagtaagcat acttttctaa ctctgtcttc aattgtttta aagaagtatt cactaattta
     7021 tcttctgtag ttgttttgac cataacttgg tttttcaaac ctttgatact ttcaatatat
     7081 aaaatttccc gaggatacag gacgagactt ccaccttttt gatcaataaa gattttatcg
     7141 acatttacga tctgctgttc tttccaattg atctgttcta gcacttgacg aatttgttca
     7201 gataaattcc tgtcttttct gatatatcca atcggaagga ttttccgatt gatgatctct
     7261 actgcaaaat tcgtataagc ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt
     7321 atttgttcgg cgacatcaat acctgtgagt gattgcttca agtcgatatc gataaagaaa
     7381 acatcgtgat tatcaatagt gcccaactgg atcaactgat tgatttgaga gatagaagtg
     7441 agctgcacga tattctcgat atgatgattg accaattctc tgatgatcca ttcgctgtga
     7501 aactggttat cttctatgat aaaatacttt ttcatatgca ggctcctatt aaaaaaatat
     7561 gtgtacattt tgaaacaaaa caatggagga ataagatggt cgcaacagtg acactttatt
     7621 tgacctttac tttgatttta gatttaggta tctatttatt cttttcgaaa caactgctag
     7681 taaaaaacag ctttggttgt atagctttga tggtggtgac acttttactt cctatcccta
     7741 aagtgagctt tccttaccta gttggcttat tattgatgtt tcttttttat tttactacga
     7801 aaaacctgtt tatcagctgt ttgatcagta atcttttttt tgctatcact ggactcagct
     7861 tttttctggt ttttgatctg atggattatt taggaaagag acatcttgga acagcaagtt
     7921 tttttgcact acttatcgca tttattttct atctattagt aaaaagtata gacaaacaga
     7981 cgagatttct caacttgctt tttacttatc caactgaaca ctattttgga ggtatttgta
     8041 ttttattaag ctggggcgtg atttacttgg tattgggaag tgttcacttc cgctcatcta
     8101 attatctgct tctatctctt ttgtcgatag tcatcagttt tttttctttg tttttttcca
     8161 ttatgttgat cagtttccaa gtaaaagaaa gacaacatca ggaatcgctt caactgtatc
     8221 aaataaatga agaatattac catcatctag aggaattcaa acatgattat aaaagtctac
     8281 tgttttcatt aaaaattact cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa
     8341 tattagaaga tcattctgca gctatttttg aaaaaccgca aatacaacag ctaacaaata
     8401 ttgtatcccc agctgtcagg ggtgtctttt taaaatttct agaaaaagca gaatcggaga
     8461 aaatcccgat ccaagtagtt attcctgtcg aagtgaaaac aatcccgatt gatctggttg
     8521 tttttattcg ctgtttatct atttttattt ctaacgcatt cgatcatcgt gtagccgatc
     8581 agagtccgat tacgatcaat ctgctggaag aaaaagaggg atcacgtttt agtatagcga
     8641 acaaggcaaa tccaacgaat ttgacgctat cggagatggt caaaagagga aaaacctcta
     8701 aaaaaaatgg cgggttaggt ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg
     8761 cagaactaaa aatcgaattt tcaaaacaga atcactattt ttttgtagaa atgtatctgg
     8821 catctagaaa gagcaaatca gcctactgac ttactgtcaa aggataagtt tgaggaaagt
     8881 ctacacggag atagagacaa caacacagca tattcaaaag aaacagaagc gtgatgaccg
     8941 ttgtcaccct tctgtttctt ttgctatgta gttaaacaaa gtagttgaca cttccgctac
     9001 ttagtattag tacgaaagta tacttcatga tactaagtag ataagaagtg aaaagcggaa
     9061 cccacgatcg tcaaaatgct aactgctttg cttacaccag ataaagggaa gagcaatatc
     9121 gcttatttta ggcgtggaga agcaagcaaa tccctacgat cacaaaaaaa atgctgaccc
     9181 agcagttaag agagttagaa caagatgaaa gtatccatcg aaaagcctat aaccaagtcc
     9241 cgcctaaagt agagtactcg ctcaccgaag aaggaaaatc attaagagag attctagtag
     9301 cgttgtctgt ttgggaagaa gaacgtatcg aaaaacaaaa agcctcaagg gaagaagaca
     9361 cgctactgca tacacatggt ggctacctaa attattgatt ttctatgaca tttattttaa
     9421 ataaaagaaa acaggagttt ttttaaaagc atcatctatt ttttatgtaa aaaaatggta
     9481 tgattaatca agtaagaagt gattggaaga aggcaagcaa atgaatagta tggaaaaagt
     9541 caaccagttt agagatgaac gtaattggcg acaatttcat aacgaaaaag atttggctat
     9601 ctctatttct ttggaagcat ctgaattgtt agaacttttt caatggaaaa cgccggaaga
     9661 agtaaaagaa acacaattag aacgaatcaa agaagaactg gcagatgtat tgatctattc
     9721 atacatgatg gcagacaacc ttcacctgga tctagatgag atcattgaag aaaaattgat
     9781 caaaaataat ttgaaatatc ctgtagaaaa aagcgcaggt gtacgaaaaa aatacacaga
     9841 gttgtagaaa tgtgtcattc ggctgttgga aagagcaaaa gaaaaacagt aaaatgagtc
     9901 tggagggaga gaaaatgaaa aaaaaaggaa aagcaaaaag atggctagtc aatatcctct
     9961 tgttcctctt gttattagta ggactagctt tgattttcaa tgaacagatc aaggattatt
    10021 ttgtcagaga gacaggagat aaatatgcta tagcgaatgt aacaaaagaa gatctgaaaa
    10081 agaataacga taaagatgtc agttttgact ttgatgcggt agaaccaatg acgacagaag
    10141 gggtcatgag gtcgcaaatg agaggaacag atctaccagt gattgctagt atcgcagtcc
    10201 cttcagtttc aatcaattta ccggttttca aaggcttgga taatacgtca ctactttacg
    10261 gagcaggaac attatctcca gatcaagaaa tgggaaaagg aaattatgcc ttagcaagcc
    10321 atcgtgcaac gaaccctgaa ttgctgttta caccgctaga aaatttagag atgggtgcca
    10381 agatctattt gacagattta gaaaatgtgt atacatacaa aacatttttt aaagaaaaag
    10441 ttgctcctac agatacccaa ttactgaatg aagtcgaggg aaaagaaatt gtcacactaa
    10501 ttacttgtgg cgacatggac gccgtcacgc gactagttgt acaaggagaa ctggaaagtg
    10561 ttacatcgat aaaagatgcg acagatgata tgagatctgc tttcaatcta gagacaaaaa
    10621 cattttagga tactgggaca taagtcgtta cagattaaaa acagccaaag gatatgaatc
    10681 gataaggaaa aaccaatttg ctgaaaaaaa gagaaacgca aagaggtgca tctctgattt
    10741 ggttgttttc tagatagact tgtgttccag tttttttatt ttttatatga aaaggcttgc
    10801 ataaataaaa attcgatggt atgatgattt tgaataagaa atggattgtt actgtacggc
    10861 aggcaaaacc agaattcagc taagaaacag taggcgaatt ctaggttttt ttatttttct
    10921 tgagagggcg aagcaaaatg aaaattgata aaattttgaa taacaatgtc gtcatttcga
    10981 aaaatggatt cggggaagaa gtcgtttgta tgggaaaagg actagctttt caaaaaaaga
    11041 ttggggacga gatttcgcca gaagcggtcc aaaaagaatt tgtttaagag attcttttgc
    11101 taaaaaccaa ttccagcagc tgatggctga tattccggca gaggagatcg aatgggtcaa
    11161 acaagtagtc gaactggctg aagataaact aaaagttgaa ttttcgccta atatttatct
    11221 gacattgacc gatcatcttc attatgcgat cacacgagca aaagaaaata tccaactgcc
    11281 taatccatta ttatttgaaa caaaaaaatt ttatccgaaa gaatatcaag cagcgaaaga
    11341 ggctcttttg ttcgtgaaac aaaagacggg tgtggaactt tctgaggatg aagccggttt
    11401 tattgccttt cattttgtga atagccaaca aggaaacgaa gacatgcaag tgacgatgac
    11461 cgctaccaca atggtaagag atatactgag tatcatcagt aaattcttcg gcatcgtgtt
    11521 cgatgaagaa tctttgaatt atcagcgtat cattactcat ctgcagtttt ttgcccagcg
    11581 ttatctcaaa ggggaaggat ctgacgagca agatgaattt ctctacgcat tagttcaagg
    11641 aaagtatcca aaagcgtttc attgtgtcga acggatcaat gagtatcttc gacaaacgca
    11701 aaaacaggaa atggggatcg cagagcagat ttatttaacg attcatattc aaagagtagt
    11761 aagtgagaaa aaaagcatcc aatcataagg attgttactg tatggcaggc aaaacctgga
    11821 ttgaaaaaat aggttcacga atgtttgtga ctgttttttt caatctgggt ttttattttt
    11881 taaataaaaa agaaagaagg aaaaaacaat ggataatcaa gcagttggtc gtcgtgtatg
    11941 ggaagcggta ggaggacaaa agaatgtcaa aagtttagta cattgtgcta cgcggttgcg
    12001 attcagatta aaagatgaat ctttagcaga tacgcaaaag ttaaaagaag acccggacgt
    12061 gatccaagtc gtacaaagtg gcggtcagta tcaagtagtg attggcagta atgtagcaga
    12121 tgtttatcaa tcgatcgtgg atgaggaagg tcttgcagtt tcggaggatg gaaaagaaga
    12181 gtccaaaaat attttgaatc gtctgatcga tattatctct agtatattta caccttttct
    12241 aggggcgatg gcagccgccg gggtattgaa aggattctta tcattggcaa cggtgatggg
    12301 ctggctgaca gctgatagcg gtacttatca aatattgttt gctgcagcag atggtgtctt
    12361 taccttctta cctgtcatgc ttgcttttac agcagctaaa aagttcaaag ccaaccagtt
    12421 tttagcagta gcaattgcta tggcacttgt gtatccagct atcacagcag ttgctggtgc
    12481 gggagaagct atcagtttct ttgggatccc agtgattctt tcaccatctg gttatacttc
    12541 ttctgtcata ccaatcattc tagccgtatg ggttcaaagt aaattagagc cattcgtaaa
    12601 aaaagtgatt ccgcaatttc tgcaaatgat tttagttccg cttgtcgttt tagtagtgat
    12661 ggttccatta acatttcttg ctttaggacc aatcgggacg gttgccggta atgctttagg
    12721 cggtctcttc aactcgattt atggatttag tccaatcgta gcaggtctaa tcatgggaag
    12781 tttatggcaa gtattcgtga tgttcggcat gcattgggga ttcgtaccga tcatgttttt
    12841 gaatatcgaa caatatgggt ttgatgtatt gatgccaatg cttcttccag ccatcttggc
    12901 acaaggtggg gctgctttag ctgtagcatt gcgaacaaaa gatacgaaac ttcgagcatt
    12961 agggatttct tctacagtga cttcgttatt tggaatcact gaaccaactg tgtatggtgt
    13021 gacattacct ttgaagaaac cattcattgc cgcttgtatt tctggaggta ttggtggagc
    13081 tattatcgga ttttccggag taaaagcttt ctccagcagt ttggtcagct tattgacgat
    13141 tccgactttc attaatacgg tggatggtgt agagtcaaat gtgacagtag cagtaatagc
    13201 tacaggaatt gcttttgttc ttgcattcgt aggtacattg attttaggat ttgacgaaca
    13261 aacacaagac aatcaattag aaaacaaaca tgcgaatgca ggagaaccga ttacttcagc
    13321 acggcatacc ttaaaaagtc ctttaacggg taaagtactg ccattgagtg aagtaccaga
    13381 ccaagttttt tcttctggcg tgatgggaaa aggtatcgct attgatccag aagtaggaga
    13441 actagttgca cctgcagacg gagagatcac gactattttt ccaactggac atgctgtagg
    13501 gatcacaacg actgacggag cggagatttt gattcatatc gggatggata cagtagaatt
    13561 aaatgggaat ggctttgaga tcttagtcaa acaaggggat ctcgtcaaag caggagattt
    13621 attgattcgt tttgacatcg aagcaattag agcagctggc tatagcgtaa tcactccagt
    13681 tgtcattacg aatactgatg catttgccga tatacttgaa ttagaccaaa aagagattat
    13741 tgcaaacgaa gatgttctgg caattgtaaa ataaggagga aatcaggatg caatcacgtt
    13801 tttcagaaaa atttttatgg ggtggagcaa ctgctgccaa atcaattaga aggggcttat
    13861 gatcgagacg gaaaaggact atccgttgca gatgctatgc ctggaggtaa gcagcgcttt
    13921 gcgattatcg gcagtgaaga atttgactgg acgattgatc aagaaaaata tatttacccg
    13981 aatcatcgag gaatcgatca ttacgatcgt ttcaaagaag atcttgcctt gtttgcggaa
    14041 atgggattca aatgttatcg tttttcgatt gcctggacac gaatttttcc aaatggagac
    14101 gaaggtactc ctaacgaagc gggattagaa ttttatgatc agctgattga tgagtgctta
    14161 aaatatgata ttgaacctgt gatcacgatt tctcattacg aaatgcctct tcatttggca
    14221 aaagaatacg gtggctggaa aaatcgaaaa ctgatcgact tctatgaacg atttgctcag
    14281 acagtattag agagatacag cagtaaagtc aaatattgga tgactttcaa tgaaatcaat
    14341 tcggcctttc actttccagc tttaagtcaa ggattagtaa aaagcaatgg agcaggagag
    14401 taccaaaata ttttccaagc atggcataat caattcgttg caagtagcaa agccgtaaaa
    14461 atcggccacg aattgcgttc ggatatccaa atcggctgca tgattatcta tgcaacaact
    14521 tacagcatcg atgccaatcc agtcaatcag gcagcaacaa tgatccaaaa ccaagaattc
    14581 aattttttct gcacggatgt ccaagtacgt ggagaatacc ctgcatacac agcgcgtacc
    14641 cataaaaaat atggagtcga tccggaaaaa ttagagcaaa cggaaaaaga cttccagttg
    14701 ctaaaagaat atccagtgga ctacatcgga tttagttatt atatgtctac ggcaatcaat
    14761 gagactgatc cacaagcagc aacttcagaa gggaacttgc tgggaggagt aaaaaatccg
    14821 tttcttgagg caagtgaatg gggctggcag atcgatccag aagggctgag aatcgcattg
    14881 aatgaattat acaatcgtta ccagaaacca ttattcattg ttgaaaacgg tcttggagcc
    14941 attgatcata tggaagagga tggcacgatt attgatgatt atcggatcga ttatttacgt
    15001 cgtcatatcg aagcgatggc taatgcagta gcagacggtg tagatttgat gggctatacg
    15061 ccttggggat gtatcgatct tgtcagtgca tcaacaggag aaatgagtaa acgttacggc
    15121 tttatctatg tggatctaga cgatgaagga aatgggacac tgaatcgatc aaagaaaaaa
    15181 tcttttgatt ggtataaaga agtaattgcc tcaaatggcg taaacttgta attaaagcag
    15241 ctgaatgaaa aagaacacac ttctcacatg tgatttgtgt tcttttttta ttttttatgt
    15301 gctattcttt tagtaatagt aaagcaaagg gtgacgttat gaaaaggaca cgtgtactat
    15361 acttggaagt cgcagataaa attaaagaag atattttctc aggaaaatat ccagtcggtt
    15421 cgatgttgcc gacagaaagt gaattagaag aactgtttca agtcagcaaa atcactgtcc
    15481 gaaaggcaat cgaactgctg gcatcagatg aatatgtcgc aaagaaaagt ggtcgtggga
    15541 cgacggttct aagcaatcgt ccttacaatc gattatccaa agcggcaagc tttacgcaaa
    15601 tcttggagaa ctcccgatat gaagtccgga aagaaacgct ggatttgcaa ttggtccata
    15661 taaagaagga agatcctttg ttttcctttt tcggtgagca agctgtttgt tttaagcgct
    15721 tatattattt gaacaatcag ccttatattt attttgttca ttatcttcca caagaaatga
    15781 tgcaattcag cagagaagaa tttggcaaag aatctttgta tcgtttgctg acgaaaaaag
    15841 gatatactat tgactcattt tctgatgatt tctcggcagt atttttaaca gaagaagaac
    15901 aaggaattct gaaaacagac gcacagatcg gcatcaaacg gatacggaaa tcgtctacgc
    15961 aaattgggga aatgattgaa tattcggagg cggtctacca tacggagctg catccttacc
    16021 atattgatta tgaaacataa ataaaaaaag aggttgtgaa aaagctgccc tgcatcaagt
    16081 aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttag gcttattacc
    16141 gcagatacac ggctgtttct ggattaccat ttattctgga taagggggtg tggcacgatt
    16201 ttcagaagga atctatgaac caatcactgt attgatttta gcttcgatgg taaccccatt
    16261 cggcttgacg atcgcttatt tcttagggaa aatcattcgt aaaaatatac tcaatcgtca
    16321 agaaatcgat acattgaaga ccgcttttcc tatggggatc tgtcagatca cagagggatg
    16381 tttccctatc gtattgaacg acttggcacg caatgtcata gcaacgggag tcggtgggac
    16441 agtaggtggc ggattaagca tgttttgggg cgcagatagc cgtattccgg catcgggaat
    16501 gtttgcagtc gctacgatga ccagaccatg ggcattcatt ggtgcattgt tagctggttc
    16561 ttttgtcaca ggaataacca ttttattatt gaaaaaacca gtagatccga atgcagaaat
    16621 catccaagaa gaaaaagagg aagaagacat atcttgggat gatttgacaa ttagttagga
    16681 gaggcagaat ggattacaag aaaaaagtac aatagatgta ccttgagaca ggaatggtat
    16741 gaaagaatca gagttagaaa gaatcgaata tacagatttt ggtttaggag cgatcgaaac
    16801 tgtccagagc atcggcaccc tccttgaaat gacgatgaag gaaaaatgag aacctttcgt
    16861 tgtcgaaagg gtctggtata ccttatgtcg aaggaaagga aacaaaaaaa ctttcctgta
    16921 ctattccaaa aaggaaagaa agttattata cagtaaaaaa agaaattatt cttcatgcta
    16981 gagatcagta cacgttcgag cccaatatca aacactagtt ccaggcaggc gcaacaggtg
    17041 cggttatcac tgaattttct tccagcagtg acgattcaag tgatattttt acaaatccag
    17101 ctatttatcg ttaacaaaaa gatttttagg agaagctgat gaacaaaaat gatcaaattt
    17161 tgatcgttta gttcgtcggc ttttttattt agagaaacat catttatcag tgaaacataa
    17221 cacgagaaca aaatgatttt ttcgttaata aaagaaacaa gtatttgttg tttaaaataa
    17281 tacacgttat tggcatatag gacgaaatcc cagtatcttc tataagtaag tcatttcata
    17341 tcgatgaaaa cacgttattt atttttgttt tatctataaa atggctgaaa aatgattttt
    17401 ttctaaatac t
//
LOCUS       DAYYCQ010000059        17350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000059 DAYYCQ010000000
VERSION     DAYYCQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.059"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..566)
                     /locus_tag="ACHAYF_001948"
     CDS             complement(21..566)
                     /locus_tag="ACHAYF_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8579381.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(578..1270)
                     /locus_tag="ACHAYF_001949"
     CDS             complement(578..1270)
                     /locus_tag="ACHAYF_001949"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8579382.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1289..1573)
                     /gene="macP"
                     /locus_tag="ACHAYF_001950"
     CDS             complement(1289..1573)
                     /gene="macP"
                     /locus_tag="ACHAYF_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8579383.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1586..2149)
                     /locus_tag="ACHAYF_001951"
     CDS             complement(1586..2149)
                     /locus_tag="ACHAYF_001951"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8579384.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2377..3039
                     /locus_tag="ACHAYF_001952"
     CDS             2377..3039
                     /locus_tag="ACHAYF_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8579385.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3163..4359
                     /locus_tag="ACHAYF_001953"
     CDS             3163..4359
                     /locus_tag="ACHAYF_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8579386.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4460..5836)
                     /locus_tag="ACHAYF_001954"
     CDS             complement(4460..5836)
                     /locus_tag="ACHAYF_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8579387.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5873..6740)
                     /locus_tag="ACHAYF_001955"
                     /pseudo
     CDS             complement(5873..6740)
                     /locus_tag="ACHAYF_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6829..7854)
                     /locus_tag="ACHAYF_001956"
     CDS             complement(6829..7854)
                     /locus_tag="ACHAYF_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8579388.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7872..8546)
                     /locus_tag="ACHAYF_001957"
     CDS             complement(7872..8546)
                     /locus_tag="ACHAYF_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8579389.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8730..9623)
                     /locus_tag="ACHAYF_001958"
     CDS             complement(8730..9623)
                     /locus_tag="ACHAYF_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8579390.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9697..11394)
                     /locus_tag="ACHAYF_001959"
     CDS             complement(9697..11394)
                     /locus_tag="ACHAYF_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8579391.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11387..12700)
                     /locus_tag="ACHAYF_001960"
     CDS             complement(11387..12700)
                     /locus_tag="ACHAYF_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8579392.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12700..16641)
                     /locus_tag="ACHAYF_001961"
     CDS             complement(12700..16641)
                     /locus_tag="ACHAYF_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8579393.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16750..17208)
                     /locus_tag="ACHAYF_001962"
     CDS             complement(16750..17208)
                     /locus_tag="ACHAYF_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8579394.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4872 a         3900 c         3012 g         5566 t
ORIGIN      
        1 tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc aaaatgtctc
       61 aatgcccatt catgtccaac gggatcgaaa cttgccacgg aatctttgaa aatatggagt
      121 ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg
      181 ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca
      241 ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct
      301 tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc
      361 ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca
      421 aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca
      481 attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg
      541 tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt
      601 cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg
      661 tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact
      721 gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat
      781 tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca
      841 atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat
      901 atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg tagccaaatc
      961 ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta
     1021 agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt
     1081 ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga
     1141 tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata
     1201 atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac
     1261 cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac
     1321 ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct
     1381 tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat
     1441 aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt
     1501 ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg
     1561 gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa
     1621 tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt
     1681 caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga
     1741 ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa
     1801 gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt
     1861 ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa
     1921 tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt
     1981 gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct ctcttcgaag
     2041 ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga
     2101 tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg
     2161 ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta
     2221 ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc
     2281 gatactcttt ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat
     2341 tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg
     2401 cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta
     2461 ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa
     2521 aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa
     2581 agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat
     2641 gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc
     2701 cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac
     2761 cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca
     2821 aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta
     2881 gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa
     2941 ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta
     3001 aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag
     3061 actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt
     3121 catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact
     3181 gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt
     3241 tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag
     3301 caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa
     3361 cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct
     3421 gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat
     3481 attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct
     3541 gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa
     3601 tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca
     3661 gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt
     3721 taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg
     3781 aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact
     3841 ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat
     3901 aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc
     3961 cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca
     4021 gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat
     4081 gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca
     4141 gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat
     4201 atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt
     4261 atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa
     4321 gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta
     4381 aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt
     4441 ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa
     4501 aataaatgat cagtccaata accattacga taccgaacac gatccatgtc acagtttgca
     4561 gacgaagcat caatacaata caagtcaaaa tggagacgat tggcaatagc ggaacgaatg
     4621 gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta
     4681 gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca
     4741 atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg
     4801 gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga
     4861 cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta
     4921 aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa
     4981 aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg
     5041 tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat
     5101 ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag
     5161 caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct
     5221 gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa
     5281 atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga
     5341 tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg
     5401 gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt
     5461 gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca
     5521 acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg
     5581 gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga
     5641 caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg
     5701 caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca
     5761 aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg atctctttct
     5821 tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg
     5881 ttttcatata ccctaaaagg gataccacct aaaatgacta aaaggcaacc aacaataaca
     5941 taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc taaagtagag
     6001 ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga tttgactgtt
     6061 agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt tttccacaca
     6121 aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat cattccgata
     6181 ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat gatggtatat
     6241 acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa atcaagcatt
     6301 ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt
     6361 acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt ccattcatgg
     6421 aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga caatggtagc
     6481 cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc aacactccaa
     6541 gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg actaacaacg
     6601 aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg
     6661 gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa catagggact
     6721 aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag aaacttatca
     6781 gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct aacgtttttc
     6841 attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc cttcagttga
     6901 ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat aaagtcctaa
     6961 tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct tatcaaagat
     7021 ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa ggctgactcc
     7081 ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat atttgatggc
     7141 attactaagc aattggttga agataaaagc gatccattta gcatcggtca aaacagtatg
     7201 atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg cctgactgcg
     7261 tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt cccgcgagaa
     7321 actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt ttgataattc
     7381 attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa
     7441 tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat aatctttttg
     7501 gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga tcagctgatt
     7561 gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat aattttgcaa
     7621 aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc tcaagtaacc
     7681 acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag caattgtgct
     7741 caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg caataaaaaa
     7801 cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat tcatcttaac
     7861 cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat atatccttgg
     7921 atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca
     7981 tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact taagatcttt
     8041 ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa atcaatgatc
     8101 tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt gtggctcatc
     8161 atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca
     8221 atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat gttcatagcc
     8281 atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac tttcgatact
     8341 tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat atctagtaat
     8401 acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc ggtcgttcca
     8461 aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat gatctcttcg
     8521 tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct tgctactaca
     8581 atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat tttagcaaac
     8641 gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat
     8701 ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg ttgtttttgt
     8761 cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa tatacctaga
     8821 aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt tgaagagact
     8881 tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa
     8941 ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa tgtatttgtt
     9001 tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg accatacact
     9061 tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag ttttttctca
     9121 gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt tggaaaagag
     9181 gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc atcaaataaa
     9241 ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg ttcgtaatat
     9301 tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt gacttgtccc
     9361 attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg cactgctaat
     9421 tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt ctcgatcctt
     9481 ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa aactgcagct
     9541 ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg ggtttgaggt
     9601 ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac tgcagcaacc
     9661 tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact tcattttaga
     9721 tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta agaatgccaa
     9781 gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta ctctttctgg
     9841 ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat aagagatgat
     9901 aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg ccatggtctc
     9961 tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat ttccatctgc
    10021 gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc ctgattcata
    10081 aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt cttcattcca
    10141 caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt tttcaaactt
    10201 tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat caaattcatc
    10261 atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt
    10321 ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg ctaatctacc
    10381 taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga caaacccgtt
    10441 gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac
    10501 ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga ttttgtaatt
    10561 tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa taggtgctgt
    10621 gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg tttcttgttc
    10681 tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat tcatctgttt
    10741 atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt atgtgtcttt
    10801 ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg gcatcataac
    10861 aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta agaaaagata
    10921 gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga ataccgcttc
    10981 tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta aaaaccaact
    11041 ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac cgtcttctcg
    11101 ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa
    11161 atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca atccgctatt
    11221 cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg tcacttcact
    11281 tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa tccctattcc
    11341 cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat tcattttttt
    11401 ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt cctagtaaag
    11461 aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct gattgacctg
    11521 gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg gtctcgttat
    11581 aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat tctaattcag
    11641 ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc acccaaaatt
    11701 cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct tccacacctt
    11761 ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc gtaacaaggc
    11821 cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca ttgtctgctg
    11881 gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa tatttccccc
    11941 cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct ttttcagttg
    12001 ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg acatttttag
    12061 gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa attggcaaaa
    12121 tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact tctggatcaa
    12181 tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta ggtgctttga
    12241 tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt accgaagtcc
    12301 ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg ttcgtttcag
    12361 cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt tcatgataat
    12421 aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc tgtgaaatga
    12481 tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca tggtattcaa
    12541 attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat tccacgacat
    12601 cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca agcggtagta
    12661 aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc cctccttttt
    12721 agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga atgcagcaag
    12781 caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt caggatcgtt
    12841 cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt ctaactcttg
    12901 ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa cttttagtgt
    12961 acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt ctcgataact
    13021 aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact tcacttcctt
    13081 gatccgatag accccttcca acaagtctga agcattgaat agggaagaat cgtttggatc
    13141 tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt tctcaattga
    13201 aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa gttccaaatc
    13261 gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga tttcatagcc
    13321 ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa acttaccttc
    13381 ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg gtgcgacaga
    13441 ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat ttaaatcgac
    13501 atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca aacgtttcga
    13561 ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg aagaatcccc
    13621 cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt ctaattttct
    13681 aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc ataaacgatc
    13741 ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt gcttgagttg
    13801 gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt catcaggttc
    13861 tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt tggtagtttc
    13921 ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa ctttctcacc
    13981 atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc tccctttctc
    14041 ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga tttccagtcc
    14101 gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat tactgccttc
    14161 aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt ccagtttgac
    14221 atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag aaagcttgtg
    14281 atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga ctctcgaacc
    14341 agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt tggctgcata
    14401 ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg gtccagcgat
    14461 ctctctgatc caaaatatgt cgctgactgc tccagtttca tctcctaaaa tcggcatctt
    14521 cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg gttcggcttc
    14581 cccactctct ggattttggt catataatgc aaattcgact ccagccaacg gctcgccatt
    14641 ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa tggaagaaga
    14701 tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt tctgaatata
    14761 ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa gttttacttg
    14821 gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt tataagcttg
    14881 gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg cttgtccatg
    14941 acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc ctggatctag
    15001 atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat taaaaccagt
    15061 ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga tatcctccaa
    15121 ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta cgccatcttc
    15181 ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc gttgtctccc
    15241 gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt tctcttgata
    15301 cttgtttttc agccaatgat gataagcaac tgtgttgatc gcagatgact tttcttgcga
    15361 agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt gagtcgcttt
    15421 attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat cttctggacg
    15481 gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag tattcccaga
    15541 aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat cttccatcac
    15601 agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa tcggtgcata
    15661 actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat ttctattttc
    15721 tccgccattg tctttataca atgtttcata gccattttta agccctagtg taagcggagt
    15781 tcctccgatt ggtgtacgat ttaatgctgt attctcactt cccgtcaaac gattgatatc
    15841 atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag ctgtactatt
    15901 atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga gcagatcttt
    15961 actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat tggtcccagc
    16021 ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga ttggactgat
    16081 ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg ttgcatcttc
    16141 aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt ttttgatgta
    16201 cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc aaagctgaac
    16261 ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat tagtagcata
    16321 gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga tcaagttctc
    16381 tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg tccaatttcc
    16441 taacactttt gtatagtcat tcgttgtaaa cttcgggtag cgatgctgtt cgttttcaac
    16501 ttctccgcta ggatagatat attcataggg gtcttgcgta gctgttggcc caagacgctc
    16561 caaaataccg ttgaataact ggcgttgaat gcgctggagt gcttgaccgc tgggtacgat
    16621 tggttcgtcc agcttttcca tctcattttc ttcgctacgt tgaatttcag cggaatcttc
    16681 tgtatcagtt tcattttctt gtaattcttg gttatctatt ctttcatcaa ccaccacttg
    16741 aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc
    16801 ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat cgatcgtctc
    16861 gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac gaacgttttt
    16921 tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac cttcattggc
    16981 actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt caatatactt
    17041 cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg tttctgttga
    17101 ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg gtccaaatag
    17161 caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat ccttatactc
    17221 ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca tctaattata
    17281 atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa aacaccaatt
    17341 atacaacatt
//
LOCUS       DAYYCQ010000060        17183 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000060 DAYYCQ010000000
VERSION     DAYYCQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.060"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1462)
                     /locus_tag="ACHAYF_001963"
     CDS             complement(251..1462)
                     /locus_tag="ACHAYF_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8579395.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACHAYF_001964"
     CDS             complement(1581..2216)
                     /locus_tag="ACHAYF_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8579396.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2352..3005)
                     /locus_tag="ACHAYF_001965"
     CDS             complement(2352..3005)
                     /locus_tag="ACHAYF_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579397.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3108..3314)
                     /locus_tag="ACHAYF_001966"
     CDS             complement(3108..3314)
                     /locus_tag="ACHAYF_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM8579398.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3390..4136)
                     /locus_tag="ACHAYF_001967"
     CDS             complement(3390..4136)
                     /locus_tag="ACHAYF_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM8579399.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4296..4520
                     /locus_tag="ACHAYF_001968"
     CDS             4296..4520
                     /locus_tag="ACHAYF_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM8579400.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4544..5305
                     /locus_tag="ACHAYF_001969"
     CDS             4544..5305
                     /locus_tag="ACHAYF_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8579401.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5317..5574
                     /locus_tag="ACHAYF_001970"
     CDS             5317..5574
                     /locus_tag="ACHAYF_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579402.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5666..5917
                     /locus_tag="ACHAYF_001971"
     CDS             5666..5917
                     /locus_tag="ACHAYF_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM8579403.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5893..6066
                     /locus_tag="ACHAYF_001972"
     CDS             5893..6066
                     /locus_tag="ACHAYF_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579404.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6180..6587)
                     /locus_tag="ACHAYF_001973"
     CDS             complement(6180..6587)
                     /locus_tag="ACHAYF_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579405.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6748..6972
                     /locus_tag="ACHAYF_001974"
     CDS             6748..6972
                     /locus_tag="ACHAYF_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579406.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6982..7176
                     /locus_tag="ACHAYF_001975"
     CDS             6982..7176
                     /locus_tag="ACHAYF_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579407.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7179..8201
                     /locus_tag="ACHAYF_001976"
     CDS             7179..8201
                     /locus_tag="ACHAYF_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8579408.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8164..8979
                     /locus_tag="ACHAYF_001977"
     CDS             8164..8979
                     /locus_tag="ACHAYF_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM8579409.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8997..9779
                     /locus_tag="ACHAYF_001978"
     CDS             8997..9779
                     /locus_tag="ACHAYF_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579410.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9779..10627
                     /locus_tag="ACHAYF_001979"
     CDS             9779..10627
                     /locus_tag="ACHAYF_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8579411.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10624..10866
                     /locus_tag="ACHAYF_001980"
     CDS             10624..10866
                     /locus_tag="ACHAYF_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579412.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11009..11377
                     /locus_tag="ACHAYF_001981"
     CDS             11009..11377
                     /locus_tag="ACHAYF_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8579413.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11392..11553
                     /locus_tag="ACHAYF_001982"
     CDS             11392..11553
                     /locus_tag="ACHAYF_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8579414.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11550..11876
                     /locus_tag="ACHAYF_001983"
     CDS             11550..11876
                     /locus_tag="ACHAYF_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579415.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11873..12688
                     /locus_tag="ACHAYF_001984"
     CDS             11873..12688
                     /locus_tag="ACHAYF_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM8579416.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12690..12815
                     /locus_tag="ACHAYF_001985"
     CDS             12690..12815
                     /locus_tag="ACHAYF_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579417.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12827..13138
                     /locus_tag="ACHAYF_001986"
     CDS             12827..13138
                     /locus_tag="ACHAYF_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8579418.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13135..13380
                     /locus_tag="ACHAYF_001987"
     CDS             13135..13380
                     /locus_tag="ACHAYF_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579419.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13377..13793
                     /locus_tag="ACHAYF_001988"
     CDS             13377..13793
                     /locus_tag="ACHAYF_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8579420.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13790..14128
                     /locus_tag="ACHAYF_001989"
     CDS             13790..14128
                     /locus_tag="ACHAYF_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579421.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14125..14355
                     /locus_tag="ACHAYF_001990"
     CDS             14125..14355
                     /locus_tag="ACHAYF_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579422.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14362..14565
                     /locus_tag="ACHAYF_001991"
     CDS             14362..14565
                     /locus_tag="ACHAYF_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8579423.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14562..14858
                     /locus_tag="ACHAYF_001992"
     CDS             14562..14858
                     /locus_tag="ACHAYF_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579424.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14935..15348
                     /locus_tag="ACHAYF_001993"
     CDS             14935..15348
                     /locus_tag="ACHAYF_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8579425.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15361..15747
                     /locus_tag="ACHAYF_001994"
     CDS             15361..15747
                     /locus_tag="ACHAYF_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579426.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16298..16525
                     /locus_tag="ACHAYF_001995"
     CDS             16298..16525
                     /locus_tag="ACHAYF_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579427.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16522..16761
                     /locus_tag="ACHAYF_001996"
     CDS             16522..16761
                     /locus_tag="ACHAYF_001996"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579428.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSSSLTELGRSRPSSIGG"
     gene            16882..>17183
                     /gene="tnpA"
                     /locus_tag="ACHAYF_001997"
     CDS             16882..>17183
                     /gene="tnpA"
                     /locus_tag="ACHAYF_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8579429.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT         5824 a         2806 c         3568 g         4985 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      541 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2341 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2401 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2461 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2521 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2581 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2641 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2701 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2761 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2821 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2881 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2941 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     3001 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     3061 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3121 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3181 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3241 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3301 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3361 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3421 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3481 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3541 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3601 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3661 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3721 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3781 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3841 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3901 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3961 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     4021 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     4081 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4141 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4201 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4261 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4321 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4381 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4441 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4501 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4561 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4621 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4681 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4741 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4801 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4861 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4921 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4981 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     5041 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     5101 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5161 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5221 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5281 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5341 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5401 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5461 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5521 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5581 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5641 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5701 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5761 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5821 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5881 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5941 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     6001 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     6061 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6121 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6181 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6241 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6301 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6361 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6421 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6481 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6541 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6601 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6661 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6721 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6781 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6841 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6901 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6961 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     7021 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     7081 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7141 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7201 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7261 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7321 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7381 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7441 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7501 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7561 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7621 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7681 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7741 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7801 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7861 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7921 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7981 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     8041 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     8101 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8161 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8221 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8281 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8341 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8401 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8461 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8521 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8581 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8641 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8701 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8761 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8821 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8881 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8941 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     9001 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     9061 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9121 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9181 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9241 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9301 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9361 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9421 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9481 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9541 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9601 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9661 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9721 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9781 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9841 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9901 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9961 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
    10021 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
    10081 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10141 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10201 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10261 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10321 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10381 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10441 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10501 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10561 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10621 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10681 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10741 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10801 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10861 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10921 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10981 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    11041 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    11101 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11161 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11221 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11281 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11341 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11401 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11461 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11521 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11581 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11641 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11701 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11761 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11821 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11881 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11941 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    12001 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    12061 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12121 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12181 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12241 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12301 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12361 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12421 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12481 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12541 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12601 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12661 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12721 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12781 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12841 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12901 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12961 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    13021 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    13081 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13141 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13201 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13261 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13321 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13381 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13441 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13501 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13561 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13621 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13681 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13741 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13801 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13861 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13921 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13981 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    14041 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    14101 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14161 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14221 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14281 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14341 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14401 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14461 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14521 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14581 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14641 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14701 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14761 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14821 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14881 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14941 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    15001 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    15061 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15121 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15181 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15241 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15301 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15361 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15421 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15481 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15541 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15601 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15661 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15721 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15781 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15841 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15901 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15961 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    16021 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    16081 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16141 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16201 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16261 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16321 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16381 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16441 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16501 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16561 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16621 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16681 acagcaaaga gcagacgctc gcgtggttca agttctttaa cagaattagg cagaagtcgc
    16741 ccatcctcta taggtgggtg atgaatgccg ttcggtatga gggttaccgt tggtaactcg
    16801 aagaccgaca actaaggcgt attgcctttt tattttgttc catgttacaa tcagtctata
    16861 caagaaatga gttgatataa catggaatta gatacaaata atcattcagt atttcttctt
    16921 tattaccacc ttgttttagt aacgaaatat cgtagacaag tgattgatga tgaaatatct
    16981 gattatgcca aaactacttt tgaaagaatt tcagaatcgt atcacattac tttagtggag
    17041 tggaatcatg ataaagacca cgttcatatt atgttcaaag ctcaacctaa aacagaattg
    17101 acaaaattca tcaatgccta taaaagtgcc agctcaagat tgataaaacg tgactttcca
    17161 agagtcaaac aatttctttg gaa
//
LOCUS       DAYYCQ010000061        16907 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000061 DAYYCQ010000000
VERSION     DAYYCQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.061"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..588)
                     /locus_tag="ACHAYF_001998"
     CDS             complement(76..588)
                     /locus_tag="ACHAYF_001998"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8579430.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(602..2899)
                     /gene="recJ"
                     /locus_tag="ACHAYF_001999"
     CDS             complement(602..2899)
                     /gene="recJ"
                     /locus_tag="ACHAYF_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8579431.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3041..3484)
                     /locus_tag="ACHAYF_002000"
     CDS             complement(3041..3484)
                     /locus_tag="ACHAYF_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8579432.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3485..4273)
                     /locus_tag="ACHAYF_002001"
     CDS             complement(3485..4273)
                     /locus_tag="ACHAYF_002001"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8579433.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4270..5211)
                     /gene="rnz"
                     /locus_tag="ACHAYF_002002"
     CDS             complement(4270..5211)
                     /gene="rnz"
                     /locus_tag="ACHAYF_002002"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8579434.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5388..5975)
                     /locus_tag="ACHAYF_002003"
     CDS             complement(5388..5975)
                     /locus_tag="ACHAYF_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8579435.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6196..7509)
                     /gene="obgE"
                     /locus_tag="ACHAYF_002004"
     CDS             complement(6196..7509)
                     /gene="obgE"
                     /locus_tag="ACHAYF_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8579436.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7797..8807
                     /gene="gap"
                     /locus_tag="ACHAYF_002005"
     CDS             7797..8807
                     /gene="gap"
                     /locus_tag="ACHAYF_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8579437.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8892..9827)
                     /locus_tag="ACHAYF_002006"
     CDS             complement(8892..9827)
                     /locus_tag="ACHAYF_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8579438.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10013..10972
                     /locus_tag="ACHAYF_002007"
     CDS             10013..10972
                     /locus_tag="ACHAYF_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8579439.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(11058..12152)
                     /locus_tag="ACHAYF_002008"
     CDS             complement(11058..12152)
                     /locus_tag="ACHAYF_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8579440.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            12341..13102
                     /locus_tag="ACHAYF_002009"
     CDS             12341..13102
                     /locus_tag="ACHAYF_002009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8579441.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(13217..13657)
                     /gene="fabZ"
                     /locus_tag="ACHAYF_002010"
     CDS             complement(13217..13657)
                     /gene="fabZ"
                     /locus_tag="ACHAYF_002010"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8579442.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            13877..14632
                     /gene="fabI"
                     /locus_tag="ACHAYF_002011"
     CDS             13877..14632
                     /gene="fabI"
                     /locus_tag="ACHAYF_002011"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8579443.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(14742..15794)
                     /locus_tag="ACHAYF_002012"
     CDS             complement(14742..15794)
                     /locus_tag="ACHAYF_002012"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8579444.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            15920..16840
                     /locus_tag="ACHAYF_002013"
     CDS             15920..16840
                     /locus_tag="ACHAYF_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8579445.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         4919 a         3642 c         2965 g         5381 t
ORIGIN      
        1 gagaatctga cttttgaggg tcgctttaaa atcgcttctt taagaataac atcaagaata
       61 gcttcttttt tctgcttaat attccattaa tgtaatgatg tcatatcctt tgattttgtc
      121 gcgtccatgg agatccatca attcgatcaa aaatgcgcag cctacgacta caccgcctaa
      181 ttgttcgatc agttcgatcg tcgctttgat tgttcctcca gtagctagca aatcgtcaca
      241 aatcagcaca cgttgacctg gtttaatggc atccttatgc aatgtcaatg tatctgttcc
      301 atattctaat ccataagtca cttcgatggt ttctcgaggt aacttccctt ttttgcggac
      361 tggggcaaaa ccgactccta actcataagc aacaggacat cctacgatga atccacgggc
      421 ttctggccca actaccatat caattcgttt ttcttttgca taatttacga tttgtttagt
      481 agcttcccga taagcgtcac catctgccat cagtggggaa atatctcgaa acacaattcc
      541 ttttgacgga tagtcgggaa tactggcaat ataatctttt aaatccactc tttgtttcct
      601 cctaggttgt taaccatttc tttatggtag gaatatcgct caaaagcaaa aattcctcaa
      661 ctttgatttt attgagccgt cgctggtata atctgctctc agtcaaagga tgactttctg
      721 ggctagcgac tttttgtaaa acaccgttct ctattgtaac aaatttcaat tcaaaaaaca
      781 cctgtatcat aaaaattaat aatttttcag ggatacgcaa atattgagct attgctttta
      841 ttttatgacg tatatcaatt cgttcttgag aatggatcaa tttaaacaat tttgcgtact
      901 gttctcttgt gcctacccca ttcaaatagg cctcatcagg tgaaatcccc atcagataaa
      961 cacgattgaa tgaataagac cgaaaaactt ctttcagatc cgctaaatca tctggacaat
     1021 ccataacaac tagagagttt ggttttcttt tttcaattat tgcctttaca gaatctagtc
     1081 cttcgaaaac aatcgcagct tcctggataa tctcaggcac gaatttcaac gtcgaagaat
     1141 caaatgctaa ataaaggcta ccttccgacg ttcggctctg ttcacggaaa cgttttgccc
     1201 gccaatcaaa tacttgcaga ccacccacag aaaaatcaga taccatcagc tgcggtttct
     1261 tacgcccgtt ccattcattg atcgaaagct ttcctaccac atctacctga ttattcagca
     1321 gttcttcttc ttgagtacca aaaccaaaag cgaccgcatc aagctgactc gatgcatcgg
     1381 aaaggacaaa tttcagatgc tgttggtttg ctccgattct tttgatattt tctgcttgga
     1441 cttgtcgaaa taaaaactga ggcaaaggat tatccgtacc aaatggcgcc aatagtttca
     1501 gctcatcaat tcgctctact gttacttcat ttggcagcaa tacttcatca attctcaact
     1561 caggccctct catcaagtca atttgatgat cgactatata ttggttcatc ttctcctgta
     1621 gtatagtgac attttcactt ggcatcgtta aaccgactgc tgcatgatgt cctccgaagt
     1681 aagtaaataa atctcgcatc tggtcaagca tttcaaaaag attcaaggct tcaatactac
     1741 gtccagatcc tttagctgat ccatcttctt ttaaagtaag gacaagcgtt ggtttacccg
     1801 tttcattcat tattttccca gcaacaatgc ccaaaacgcc ttcatgccat cctcggttta
     1861 ccaatagatg gatatgattc tccttattga ccatcgctaa tgcttcttgt gttatctgct
     1921 ctacgattgc ttttctttct tcattgatct cattcaattt ttttgcttga acggatgctt
     1981 cctcttcatc aaacgtcgca agtaacgaga cggcaggacg tggatcgccc atccgaccga
     2041 ttgcatttag tctgggagca atcgaaaaac cgatcgttgt ttcgtctgcg tccctcattt
     2101 ttacgccgct ttcttcaaat agcgcctgaa gtcctatccg ttctgaatga tgaatggcat
     2161 cgattcctaa tgaaacaagg attcgattct catcagtcaa tgaaactaaa tcggcgatcg
     2221 ttcctatcgc tactaaatcc aaaaattctg caggcggttc ttccaataat gcagaagcta
     2281 ctttgaatgc tactccgact cctgccaaat ctggaaatgg atagtgcccc gctggatgac
     2341 gagggtgaac aatcgcataa gcgtctggta aattctcagg tagttcgtga tgatctgtaa
     2401 tgatcacgtc tacaccagct tgctgagcgt atgctaccgc atcatttcct gctaccccgt
     2461 tatcaacagt gatgatcaac tggattcctt cttcgatctt ttcttggtat accgattggt
     2521 ttggtccata cccatgctca aatcgatttg gcaggaatac ttctacttct gcaccaagta
     2581 attccagtgt ttctttcatt accgttgcac tggtgatccc atctgcgtca taatcaccgt
     2641 aaacgaggat tttttctcca ctgattacag cttcttggat tcggctgatt gctttatcca
     2701 tatcgtgtat cagatacgga tcatgtaatt cttgttcctt tggatgtaaa aatcggttga
     2761 tatccttttc ctcatggtaa cctctttgcc acagtaactt accgacaaat ggagataatt
     2821 tcagttcacg gatcatttgg agaaaggatt cagaaggctc tgtctcgctt aacttccaat
     2881 caaaatttgc ttgtctcact ttctcactcc taacttagtt attatagcaa aggaaatcag
     2941 caaaaaaaag cagaaagaaa gaataaaaac agatcagatc cgctagacag atgttcagca
     3001 ggacctgatc ttttgtacga ccatttatga aatatcccta ttagtcaaaa taattgaagc
     3061 tttgtttggg cgattgagtt ttttgttctt ccggcgcttt caattgactg atttgtgcgg
     3121 ttttcgcttg taactccgcc tgataagcag cttccaactc cgcacgttcg tttctaaact
     3181 cgcgttgctg ttcttctttt gcttcttcaa ttttcttttg aagttctgtt tgatattctg
     3241 acatttcctg agtcagctgt ttgatttgct ttttttgctg ccatagtgtt gccgaagcag
     3301 tcaaaaatac aatcagtgca ccaatcagcg cagaaccgat gatcactaag atcaatggcg
     3361 cagaaaagga agtgaagcca aaacttacag gaacattttt attgttcaat acagcaaaaa
     3421 gaacaataat caaaactaaa acaaatccaa gaatcacatt gctttgcttt ttcaattcct
     3481 tcacctactt tttgttaaaa attccgctgg ctaaataatc accaatcgtt ggaaacagcg
     3541 tataaaattt tgatgcaact tctaaaataa acggttgatt gatttcccgt tttggatgtc
     3601 tcatattgcg gacgatcatt tttgccaact tggcaggttc taaaacgatt tgtccgactt
     3661 tttccaaata gcttccgctt ggatcagcct tatcgaagaa atttgtctcg attggacctg
     3721 gattcactgt cgtaacagct acgccaagtg gtttcaattc taatcgcaaa gcgttggaga
     3781 agccaaggac tgcaaatttc gtggcagaat aaaccgttga ttttgcagtt gccatttttc
     3841 cagccataga ggcgatatta atgatatgac cttgtcttct ttctgccata tcgatcgcaa
     3901 atttttgtgt caaaaccatc attcctaaga tgttgacttc aaacatatcg tatgcttttc
     3961 caaggtcgaa tgtaaggaag tcttcgaaaa tcccaaaacc cgcattgttt accagaatat
     4021 cgacgcgccc gacttcttcc atcacctttt catataattc gtccacgctt tcaggattgc
     4081 tgacgtctac cttgaaagca taagcctctt tgccgctcaa gcgtctgcac tcgtctctga
     4141 caccttctac aaatgccatt cttcgtgcgc aacttataac gattgctcct tgctttgctg
     4201 cttcataaca gatttgtgcg cccagaccgc ctgaactacc tgttactaaa acaactttat
     4261 cttctaaatt catgcctctt cctcctcgcg aaatggaatc tcaatgctat caaaatcttt
     4321 cataatcgtc gtgttttcaa acacttcttg tgcttctttt tcaagctcta aagctgcttt
     4381 tcctaaatat ctcgcactga tatgagtaag tagcagccgt tttgcctgcg cttcttttgc
     4441 aacttcagca gcttgatgag tcgtggaatg gaaatagttg tgcgccattc ttgcttcatc
     4501 tttattaaat gtgctttcat gtaccagaat atcgctattt tccgcaagca caacggaatt
     4561 atgtgttttt ctcgtatcgc ctaaaatagt cacgattcgt ccttttttat ctggacctac
     4621 gaaatctttt ccattgatcg tccgcccatc ttctagttgg atcgtttcac cttgcttcag
     4681 tttgccgtac agtggtccag ctggaatacc taattctttt agacgatcca cctgcaattc
     4741 tcctttatga tcatgttcaa ccacacgata accaaaactg tcgatcccat gatttagttt
     4801 ttttgtataa acagaaaatt gctgatcagt aaaaatcggg gctgtttcat ttagctcaat
     4861 gaatttcagc ggataactta aacgcgtttg cgaaatacct aaagagactt ttatgtattc
     4921 ctctatccct tttggcccgt aaatctctaa tggcgtatcc ccaccttgaa atgaccggct
     4981 gctgatgagc ccgggaagtc caaaaatatg atcgccgtgt aaatgtgtaa tgaaaatttt
     5041 accgattttt ctcggacgaa tagtcgttcg caaaatctgc atttgcgtgc cttcgccaca
     5101 gtcgaacaac caaacttcat tgcgctcgtc taaaagtttc aatgctatac ttgtcacatt
     5161 tctatgtttt gcaggaacac ctgcacctgt tcctaaaaat tgtaattcca taatctacct
     5221 gactttcttt aagttcctct ttaattttag ggtaaaaaga agggataatc aaccgtaagt
     5281 cgagaatggg ttcaaattaa aaataacgtt ctaaagcgat agcgttttct ttgaaaatta
     5341 ctataaaaac agcagcgaac ccgtttgaga tcctctgctg ttttttatta atttgaagat
     5401 ccagaacttt cattgtcgtt ggaagtttgc ggatgtttgt ccttccaagt cttttgtgca
     5461 gcttctttcg catgattttc agcttctttc gcatcatgca catctttttc gacatcttca
     5521 taatccaagc gagtaatttc tccgcctaat tccatcatta ccaattgatt cagtgggcga
     5581 ttgtcctctt catcagcaat caagatcagt cctgtagctc cttcaccgat tttatttcct
     5641 acaaagtcga acacggtctg ggcatctttt atttctttgg catcttgtgt cgcaccgatc
     5701 attcctccgg caaaccatcc taataaaatc cctagcggac cacctaatac accaaccaac
     5761 attccgatta atccacccgt agaagatttg ttattaccgg taaagtcgat aaaatcttct
     5821 attttgaatt gatgtgtccc attaggatca tggctgacaa ctgccatctg ttctccttta
     5881 atcgaacgaa cagcatgtaa ctttttgatt tctgaaaatg cctgatatgc ctggctttct
     5941 acttgaaaat ttaaaatgat tactcgtttg ctcattaaaa acgccccttt cataccttct
     6001 tgttatttat tttaccttta attgaaaaat aagaaaagaa aagcactctt tttttccaat
     6061 aaaatagggc tgtgacaaaa gtcgtatcac agctcctttt tccgaataaa cggcgttctt
     6121 gaagctgatc acttctgtca caacctccct taaatctgaa tctatatttt ttggtatttg
     6181 tttttatttg cttgcctatt cgacaaattc aaattcaaac tcaccgatac gtacaaggtc
     6241 gccatctttc gcaccgcgtg cccgaagagc ttcatccaca cccataccac gtaattgacg
     6301 agcaaaacgc atcactgttt catcatgatc gaaattcgtc atttggaata atttttcgat
     6361 cttctcacca gataagatcc acgttgcatc tgaatcacga tcgatttgga actcaggacc
     6421 ttctggattg aatccatagt gtacagtttc ttcttccagt tcttcttcgt acaatgggaa
     6481 ttctggcgtt acttctaaca aatctgctgt agcattcaat aacgcgtcca atccttgtcg
     6541 agtcactcca gaaattggaa atactgggat gtcatcagca aattcatctt ctttttcctt
     6601 gttcagctgt tctttgaatt tcactagatt ttccggtgca tccggcatat ccattttgtt
     6661 tgccacgatg atttgcggac gctctagcaa gcgtaagtta taagtagata attctttgtt
     6721 gatggcaaga tagtcttcat aaggatctcg tccttccatc ccactcatat cgatcacatg
     6781 taaaattact cgtgttcttt cgatatgacg aaggaattgt gtacccagcc caacaccttg
     6841 cgaagctcct tcaatcaagc ctggcaagtc tgctgcggca aaacttctgc catcacttgt
     6901 agtaaccatt cctagattag gtacgagcgt cgtgaaatga taagcgccga tttttggacg
     6961 agcagatgaa ataacggata gtaaagtcga tttcccgaca gatggaaaac ctacaagtcc
     7021 aacgtctgcc aatactttca attctagttc gatttttcgc tcttgtcctg gttcaccgtt
     7081 ttcagcaatt tccggtgcag gattccgagg agaggcaaaa cggatattcc ctcgtccacc
     7141 gcgtccacct tttgccacaa ctagtgtttg accattttca attaaatctc ctaaaagagc
     7201 accagtctcc gcatctcgga cagtggttcc ttgtggtact ttaacatagg tatcttctga
     7261 accacgaccg tgcatccctt tactcatccc attttcacct ggctgcgctt tgaaatggcg
     7321 attgaaccga aaatccatca atgtacgtaa cccttcatcg acgatcaaga tgacatcgcc
     7381 gccacgacca ccgtctccgc cagccggtcc gccgtcagga acgtattttt cgcgacgaaa
     7441 tgcaaccatt ccgtctccgc ccttgccagc tttcacatcg atcgttactt gatctaaaaa
     7501 catggacata attaagtcct cctattattc atttatttat ttgactgcct gaatttccac
     7561 aataaacaaa agtcttactt attttatcgg tgaaatctga ttttgtctat catttcagca
     7621 aaaagattgt ttaccttctt ctttttttat catttggtaa ggtttccaac cttttttctg
     7681 ctaccaagta taattttttg acaaaatatt ctttgcgtag cagaataaca gtgtattaat
     7741 tgtattaatt gaggatgccg tttcacagca cccctaataa aggaggaact tttgatatga
     7801 cagtaaaagt aggtattaac ggatttgggc gtattggccg tctggctttc cgccgcatca
     7861 aagaagtctc agatgatatt gaagtagtag caatcaacga cttaacaagt ccaactatgt
     7921 tagcacaatt attgcaattt gactctactc atggtactta tcctggcaca gttactgcaa
     7981 cagaagactc aatcgtagtg gatggcgaag caactcgtgt ttatgcagaa cccgacgcaa
     8041 gcaaaatccc ttgggcaaaa gaaaacggtg tagatatcgt actagaatgt actggttttt
     8101 atacatctga agaaaaagca aaagcgcatt tagatgctgg tgtcaaacga gtagttattt
     8161 ctgccccagc tggtgctatg aaaaccatcg tctataacgt caatgatgat acattagatg
     8221 aaaacgacaa aatcatctca gcaggttcat gtacaacaaa ctgtttagca cctatggctt
     8281 atttcttaaa taatgaattt gggatcgaag ttggtacaat gacaacagtt catgcctaca
     8341 cttccacaca aatgctctta gatggacctg taaaaggcgg taatttgcgt gctgctcgtt
     8401 cagctgctga caatactatc ccacattcta ctggtgctgc gaaagcaatc ggcttagtta
     8461 tccctgagct acaaggaaaa ttacaaggac acgcacaacg tgttccagtc gttgatggtt
     8521 cattaactga attggtttca gtattgaaaa caaaagtaac tgctgatcaa gtaaacgaag
     8581 caatcaaaaa acacacagtt gacaatcctt cattcgggta tgatgaccgt caaatcgttt
     8641 ctagcgatgt cattggaaca actcaaggtt cgatctttga cccaacgcaa acagaagtaa
     8701 caactgctgg tgatttccaa cttgttaaaa ctgttgcatg gtatgacaat gagtatggat
     8761 ttacttgcca aatgattcgc ttactagaaa aattcgctaa cttgtaattt gatagatcaa
     8821 tcgataaaag aagcctttga ctggatccag tcaaaggctt ctttcttttc atcccctact
     8881 tttaaactcg ttcaatgaac atattggctt aaaaattgtt ggactacttg ctgatatttt
     8941 tcaggatctg tcttgtatgc ttccgcatgc tcggctcctt ttacaacata cttttctttt
     9001 ggtcccttcg ttgcacgata gaccttatct aacatttcaa atggtacaaa tgtatcttcg
     9061 tctccatgta taaagagcat tgggcgctgg tttttcttca attgttccac tgcatttgct
     9121 tcaccgaaaa agtagccagc acggactttt gtcactagac tcgttacttg cataagcgga
     9181 aatgctggaa gactaaacat atcgtctagc tgataagcaa gctcatcatg aacggaagaa
     9241 taaccgcaat cttcaatgat tgctttcaca ttatctggta gtttttctcc gctagtcatc
     9301 ataactgttg ctgctcccat gctgacaccg tatagaacga tttcttgcga ttttccattg
     9361 ttttctagca ctttgtttat ccactgtaca tagtctttac gctctggcca tccaaatcca
     9421 atgtaatccc cttcgctttt tccatgtcct cttgcatcag gaacaaggac attgtaaccc
     9481 atatcatgaa acatctttgc gtaattggtc atcgtttcag cgtttcccat atagccatga
     9541 gccacaagag ctgttttttc acttttcgtt tcagcaggaa gataaatcgc tgataatttc
     9601 agaccgtctt ttgaagtgat ggtccaatcg cttcggtttt cttttgaact aaaccattta
     9661 tgtgcatccg tttctttgga tgtctgtgtt gtaccaggtg tatcttcgtc taaaaagtct
     9721 ttctcagaag gcacaacagc ataattataa aaataattcc ctgcaaacac taacgcaaca
     9781 atcagagcta aagcgacgcc taaaaggatt ttcatccaaa gtttcataaa tcatccatct
     9841 catttctctt ttttcatata accttgaatt agtataatca aagaatctct actatgcaat
     9901 gttcattttt ataacccgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg
     9961 gtacactcaa attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta
    10021 caaacatctt accatagacg aactgacaat gatagaatca tattatcttc aacataataa
    10081 gccggttgaa atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa
    10141 taagttcaag caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa
    10201 aaaatgtggt agaaaagtca ttcaattacc tgctcatgaa gtagattaca ttaaagagaa
    10261 agtcactctt ggttggacgc ctgacgtcat tatcgggcga aaagaaaggt ctgtttcatg
    10321 cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg acacactacc
    10381 gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac aacagtatca
    10441 gcgctcaatc catgatcgac ctgataatta tcctgatttc aattctgagt ttggtcacct
    10501 tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag
    10561 attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac
    10621 tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgtttga
    10681 ctgtggaaaa gaattttcta actggaaagc cattagtaac caacatgata ttgatatata
    10741 ttttgcggac cctggaacac cttctcaacg cccattaaac gagaattcta acgggattct
    10801 gcgtcgtaat ggactgccga aatcaatgga ttttagaaaa gtgaatcaga catttatttc
    10861 cagtgtcagc aatcaacgta atcatattcc aagaaaatca ttgaattaca gaacaccaat
    10921 cgaaatattt ttgagctatg tacaagaagc attttattct agcttaattt gacaaatcat
    10981 aacctataaa aaaacaaaat aaaaaagcag aaataactgg ctatttctag cggattattt
    11041 ctgcaataac taattatcta ttttttattt tcaattaatt taaagcttga ccatggtttc
    11101 aaaaagtcaa ggaggaataa ttcgtcatcg gagattcttc ctacaacatt gacacgtccg
    11161 tcgtttttca tatccttcaa ggcgatttgt gtttctcctt tgtattgacc gtacccttca
    11221 ttgtcaatca gtacatcccc atgatgcata tctcgcgtat tatgaggcgg gaaatcttta
    11281 tctttgtagt atacacgagt catcgttgaa cgaaggatat attctgagcg atcccctcga
    11341 taactatgaa ggttatcaaa caagcagatt cgttcgtttt ctgtaatacc ttcttccgta
    11401 tctactttca atgttggata atccgcgtta aatgcttctg ccatttcttt caattcttct
    11461 tcacttgcgt acgcatttcc aatagaaata tcatcaatca atcctgtaag gattaaatgc
    11521 ttcacttgtg tcgcaatttc aagatctcgg tggtcttcca gcgtgcacaa gccatcttgc
    11581 gtaggccaag ggccaaactc agcagattgc gaattgacga atgccatcgt attcaaatta
    11641 tattttctga atttttctga acaaaaaaca aagtgttcgt atcctaaacc agagtatcga
    11701 tgaggataaa aattatgaga acctaataaa ttatccgtgt ttggcgaata actcatgatg
    11761 ttgtcaacgt aattcgtgcc cgaactcata ttgatctcga ttttgatacc aaaaggattg
    11821 cgcgtcattt ttgcttcttc tgctcctgtg aatccgatat ctaagcggac cccgtaggca
    11881 cccatcttat ggaaaaaaga taagtcatcg taagaaatct ccaactgttc aaataacgct
    11941 ggattgatat caaccattac ttccatgcct aggctattcg cgtaatcaac tacttcttta
    12001 aattctgaaa gaactttttc tttatcatca ttgatctgca gcaaactggt aaaaacccgt
    12061 ttatacccat acttatgagc taaatccaag tacgctttgt ccttttcaaa tgttgaccgt
    12121 tctggataaa tcgagattcc taattttccc atattaccat ttcctcctgc atcttttcgt
    12181 ttttgtatcc acttacatta tagcaaacac aaagtaaatg gtctacacct tttctttttc
    12241 ttttaaaata aatgataaat tccaataatt taagtaggta ttttccctct attttgttat
    12301 cattaaagat aacaaaatag taaattggaa aggaacagtt atgaagaaaa ataaagttat
    12361 tttaagtatt agtttagcag cagcattgct gtttgctgct ggttgttctg caaacaacac
    12421 gacttcttct agttcgtcta cagaaagttc aaaaagtaca tctactgcgg aaagcacgac
    12481 tgccagtagc gtcgatttga acgcattgga attaccacaa ttgacgaaag atgtcgccga
    12541 aaatgaagat ctggtcgaaa tggtgacgaa taaaggcaca atcaaaatca aattatttcc
    12601 cgaacacgca ccaaaagcgg ttgaaaactt tatgacacat gcaaaagacg gttactacaa
    12661 cggactgact ttccatcgcg tcatcaaaga tttcatgatc caaggtggcg atcctaaagg
    12721 cgatggcact ggaggggaaa gcatttgggg agaaggattt gaaacagaga tcaacaacca
    12781 cctttataat ataagaggtg cgttatctat ggctcgctca caagatccta acagtaacgg
    12841 cagccagttc tttatcgttc aaaatacaga tgatatgcat gatggtcttt taaaagacga
    12901 ctatcctcaa gcaatcatcg acgcttataa aaacggcggt tatccttcac tagatggcca
    12961 ttatactgtc ttcggccaag tgattgaagg aatggatgtc gtggatgcta ttgcaaatat
    13021 cgaaacagat agttcagaca aaccaaaaga agaagtaaaa atcgataaaa tcaatatact
    13081 gcaagaagca aaacaaaaat aaaaaaataa cgtctgaatc ctgcatagat gacaacgtat
    13141 gctaggattc aggcgtcttc tttttacata aaaagcaagt aagcttcctt ttgttttgct
    13201 atatcaggtt ttatagtcaa gcattttggt tgacgatgaa cgtgaactcg cactcacaga
    13261 cttttttatc atctacatat gctgcagata acgctgtacc gactgatccc ttcattttgg
    13321 tgatttcaaa atgacatttc agcacatctc ctggaacaac tttttgacgg aatttggcct
    13381 tattgatccc accgatataa gctctttttc ctagaaactc ttctgatttt aggataagta
    13441 tcgaacctgc ttgtgccagt gtttctagaa tcaacgcacc cggcatcact ggattgtcag
    13501 gaaagtgacc ttggaaaaaa tcttcattga ttgttacatt ttttgtcgca acgatctttt
    13561 ttcctggttc taaagtatct acataatcaa tataacaaat aggataacga ttaggaatca
    13621 attccattac ttcagtagca gataatacgt aagacataat taaactcctt ctttcaggca
    13681 attgccattt tcattacttt gactatcaaa gtatcatatt cctaaatatt tagcaatctt
    13741 tttttaattt aagaaaaaat agtttgacaa tcaaaacatt ttgactaata attaagaaat
    13801 caagaagtta ttttagctat tattctctat ttttttacga ctatcaaatt tttatcagaa
    13861 acaaaggaga gtcattatgt catttctaga aggaaaaaaa atcgtcatta tgggcgtcgc
    13921 taacaaaaaa agcattgctt ggggttgtgc tgaagcaatg ttgaaacaag gggcagaaat
    13981 tatctatact taccaaaatg aacgaatgaa aaaatcatta gtcaaattgg tcggcgaaga
    14041 agcattcact gtagaatgtg atgtttctag cgacgaaagc atcgaaaaag caatgaatgc
    14101 catcggtgaa tatgctgggg agatccacgg ccttgtccat gcagttgctt acgccaacaa
    14161 agaagaattg tccggaaatg tgtctgatat ttccagagac ggctatcaat tagctcaaga
    14221 tatcagcagc tactctttaa ttgctgtatc aaaatacgca caaccgtttc ttgcaccaaa
    14281 cagcgggatc gttagcatga cttatctagg ttcagaacgc gcaatcccga attataacat
    14341 gatgggtatt gcgaaagctt ctcttgaagc tgctatccgt tatttggcag ctgagttctc
    14401 gcctaaagga attcgggtca atggaatctc agctggggca atcaaaacat tagctgtcac
    14461 aggtgttaaa gactatcaaa aactgatcca gttatctgaa tctcgtacgc ctgatcagca
    14521 aggtgtgacg atcgaagaag tcggtaatgc ttgtgctttc ttaatcagcc cactttccag
    14581 cggaatcatc ggtgatatta tctacgttga caaaggtgtc catctttcat aaacttttgt
    14641 tgcattcttt aaagtaaaac atataaaaat caggaaaaaa ctttttaaag tctcttcctg
    14701 attttttctt tttacgtttt tttcaactca ttactttgaa attattccac gacttcaatc
    14761 cagccttctg gtgcttctat gtctccaaat tgaatacctg tcaattcatc atacaaccgt
    14821 tttgttactg gtcctacttc cgtttcacta taaaatacat gaagattgtt attgtgataa
    14881 atcccaccga ttggagaaat aacagccgct gtaccacatg caccagcttc tgcaaattga
    14941 tctagatcat caatgaaaat atctccttca attgcttgca gtcctagtcg ttcttcagct
    15001 aactgcaaca aggaatattt cgtgatactt ggtaagatag agggcgattg cggagtgatg
    15061 aattttccgt cttttgtaat tccaaagaaa tttgctgcac ctacttcttc gattttcgta
    15121 tgtgtggctg gatcaagata aacacaatct gagtaatcca gttcttttgc ttctgttcct
    15181 ggaagtaaac tcgctgcgta atttccgccg acttttgctg caccagtccc cttaggagca
    15241 gcacgatcgt aatcagatac aataaaattc gttggtttta gtccaccttt gaaatacgct
    15301 cctactggcg tacagaagac gacaaataaa tattctggag ctggatggac accaatgttt
    15361 tcaccgaccc caatcagtaa agggcgcaag tataatgtag cgcctgttcc atagggcggc
    15421 acaaaatctt catttgcttt taccacttgc ttgacagcat caacgaacat cttttcaggg
    15481 accattggca tcagcaatcg tctagcactt tgattcatcc gtcgactatt ttgttcaggt
    15541 cgaaagagat tgaccttgcc gtcttttcga cgataagctt ttagaccttc aaaacattgc
    15601 tgaccataat gaagcgctgg agagccttca tgaatcgtca ggtaattatc ttctgtcaat
    15661 tctccttctt cccattgtcc gtctttccat ttggcaataa aacgccaagg agttttgata
    15721 taagaaaaac ctagattgtt ccaatcgatt tgcatatact taccaccttc gttattttct
    15781 ccacatttta tcattatcca tattatttgt cattttattt tcagcttttt tccttcatta
    15841 tagttatttt tgctatttcc ttttttgaaa acttcttggt acaatacaga aagaatcaaa
    15901 ttttagaaaa gaggatttta tgttttcatt attagcaact gccggtacga cagactcttc
    15961 tggcgagaca ttgacagatc aagctgtcag caaagtaaat gcgtttcagc ggttttggga
    16021 cgggattgat tgggacgcca ttttggctgc ctttatccaa aaaagtttat atctgatttt
    16081 tttgatcgtc cttttttgga ttcttcaccg tgtgggaaaa tatctgattg ataagtcttt
    16141 taatcagtat tctaaaaaag ccattctaaa cgagagccgc ttaaagactt tacatagtct
    16201 tttaaatact atttttcatt atacccttgg tttctttttt atctatgcaa ttttatctgc
    16261 tatcggtgtg ccgatcggat cacttttggc tggtgccgga attgcaggtg tggcaatcgg
    16321 tcttggtgcc caagggttca tgagtgatgt tattaccgga ttttttatta tcatggaaca
    16381 acagatggat gttggggact acatcaagct agcaaactta acgattgaag gaaccgtcgt
    16441 atctgttggt atccgtaccc ttcagctgaa agcaacggat ggtactgtcc atttcatccc
    16501 gaatcgaaac atcaccacga tcagcaatct ctctcgtgca aatatgcaag tacttttaga
    16561 tattcggatt gttcctgaag aaggatatga caaaatctat gagatcattg atcgagtaaa
    16621 tcgtacatta gctgaaaaat accaagaaga gctccaaact gaaccttcta tctttggatt
    16681 agtggatctt ggaaacagca attttgcgat tcgtacggtc tgctacgcat tgaacggaaa
    16741 acaatatgcc ttaaaagaag agttcttgag ctcctatgtc aaagaattga cagcttctgg
    16801 tttcacgatt ccgaacagtc caattgctgt cggaaaataa tttggacaaa cgatgcaacg
    16861 aatctcacta aaaagagcgt gggacaaaag taaagaacac ttttgtc
//
LOCUS       DAYYCQ010000062        16894 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000062 DAYYCQ010000000
VERSION     DAYYCQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.062"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..549)
                     /locus_tag="ACHAYF_002014"
     CDS             complement(<1..549)
                     /locus_tag="ACHAYF_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8579446.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDE"
     gene            complement(797..1633)
                     /locus_tag="ACHAYF_002015"
     CDS             complement(797..1633)
                     /locus_tag="ACHAYF_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8579447.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1644..2573)
                     /locus_tag="ACHAYF_002016"
     CDS             complement(1644..2573)
                     /locus_tag="ACHAYF_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8579448.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2765..3118
                     /locus_tag="ACHAYF_002017"
     CDS             2765..3118
                     /locus_tag="ACHAYF_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8579449.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3192..3848)
                     /locus_tag="ACHAYF_002018"
     CDS             complement(3192..3848)
                     /locus_tag="ACHAYF_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8579450.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4074..6008
                     /locus_tag="ACHAYF_002019"
     CDS             4074..6008
                     /locus_tag="ACHAYF_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8579451.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6094..7317)
                     /locus_tag="ACHAYF_002020"
     CDS             complement(6094..7317)
                     /locus_tag="ACHAYF_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8579452.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7311..9182)
                     /locus_tag="ACHAYF_002021"
     CDS             complement(7311..9182)
                     /locus_tag="ACHAYF_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8579453.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9203..10321)
                     /locus_tag="ACHAYF_002022"
     CDS             complement(9203..10321)
                     /locus_tag="ACHAYF_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8579454.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10404..11237)
                     /locus_tag="ACHAYF_002023"
     CDS             complement(10404..11237)
                     /locus_tag="ACHAYF_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8579455.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11230..12147)
                     /locus_tag="ACHAYF_002024"
     CDS             complement(11230..12147)
                     /locus_tag="ACHAYF_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8579456.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12374..13498)
                     /locus_tag="ACHAYF_002025"
     CDS             complement(12374..13498)
                     /locus_tag="ACHAYF_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8579457.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13681..14589
                     /locus_tag="ACHAYF_002026"
     CDS             13681..14589
                     /locus_tag="ACHAYF_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8579458.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14730..16343
                     /locus_tag="ACHAYF_002027"
     CDS             14730..16343
                     /locus_tag="ACHAYF_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8579459.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5336 a         3292 c         2794 g         5472 t
ORIGIN      
        1 ttcatctttc aatactaggc ctattttatt tttataacct ggcatgctca ttagataaag
       61 gatttcatcg taatggatat agtgatcgct attaccataa cggacagttg tccacctttt
      121 tttcgtagca tgttgaacag gatagttttc tttttctttc aataagtaac tttggatgct
      181 ttttttattt ttttcataag tgaattcttt tatcaaataa ccactaggcg taatctttcg
      241 attaataaca gttattgctt tattcggatg agacgttaga taaacaattt tacacttttc
      301 attcttttct ctaagcattt cgcctagttc gataccgcta taataggcgt tcaaatcgat
      361 gtctaggaaa aaaatatctg aggaatagat ttcattattt ttaatgaatt caacaaattg
      421 tataacgttc aatccctgaa cgatctcaaa tttccaagga cttaataaaa ttctggattc
      481 ttccaaaatt tctactagca ttttttcaaa aaccaaacta tcatccaaaa taaatatacg
      541 cgttaccata aaaagtacct ccactcgata ttttactatc aaaatcattt catacaactt
      601 ttcaacatac atgttaggaa taaattgtaa ttctcttcac aatcttttcc aacacctttg
      661 aaaccgatat caaatacttt caggatgctg cttctaaaat aaatatcaaa caaatgaata
      721 catcatgcaa aaaaagccag acaaccgacg tccttgcgtg tcagctgtct gagctatgtt
      781 tatttatttt gttcttttaa aaagaacgat acacatactg atttttcgga gcagagactt
      841 tctcagcgtt tgtaacaact acagatggga tttcacgctt gaatggttga taatagtctg
      901 attggatggt tccttctatt ttataccatt catcattttt aaccgttact ccttccggca
      961 tatgcgtcaa caaaccaaag actcctgaat ctgccacaca atggataatg ccgaatcgga
     1021 acaagaagat atctgtattg ctatcgttcg gtgtttggta aacaaatcct tcatagctga
     1081 tttttttgcc aatgaattcg ctagggtaat tataaattag ctccataact tctaaatagt
     1141 tttcatctgt gatcttgata gtttgcttat ccccatactt ttctttcaac tcattcattt
     1201 ggtctaagta gtctgatttg ttgaaataaa tactggtatc cggctttaag tattgtgtct
     1261 gcatctccgg atcccctact gattctttac ttactgggaa attaaatcct ttggcttcta
     1321 cgatcgtagt gtcgagacta actgttggaa acaacgtccc cacgattact ggcaaagcta
     1381 gcagtacata agcgattcca cgttgataag gttttgacaa cccatggtca tgatcgtgct
     1441 cgtgtgcctt ttcttctgct tttccgtttt tgacccacaa atacagctga actaaagcca
     1501 aaagagcaga taaaatcatt gaaagaatag ctaagtagcg ataatggaca ttgatatatt
     1561 gatcgagttt gccagaaact tgcaagtaca tcatcaattc gaaataccca gataaaatca
     1621 aaaaccgcag catattttcc ctcctaaata aataatgtga atccaataac gctaagggca
     1681 atcagtccga tcagcgataa aataaagcgt cctgtaaaat atcgtttcat catcagtaga
     1741 tttttgatat cgaccattgg gccaaagacc aaaaaggcag ctacaggtgc tgtgccgaac
     1801 agacttaaca aagaagatcc tataaacgca tctgcttctg agcaaagaga catcgtaaag
     1861 gccaataaca gcatgatgat aatggctaaa atctttgttg agccgagcgt tagcataatt
     1921 ctagttggta aataagtctg catcgcagct gcgattaatc cgccaatgat caaataacga
     1981 cctgtatcaa aaaattcgtc gattgaatgt gtcaacacat gcccagtttg atggatcagc
     2041 tgattttttg gttcattctt ctcactttgt tcttcttcat ggacatgatg atggtgattt
     2101 tctgctactt tagacgtgat tttttctttc aatacagcgt ctttttgaaa ataggcaagc
     2161 cagacaccaa tcacaagggc aacaaaaaag ctgcctatca cccgccagct tgccatctgc
     2221 caagaattgc caaatgcgat aaaagtggaa aaaatcacga ttggattgat gatcggagca
     2281 gtcagcataa aagcaaacgc tgtgtgaact ggtacgcctt ttttaacaaa ttgatgaacg
     2341 atcggtacga tgccgcattc acatgaagga aagaaaaatc ctaagatcga accagtaaaa
     2401 atagagaaga tacgattttc aggaagccat ctcttcacac gttctggggt caaaaatact
     2461 tgtaaggcgc ctgagataag acagcctaat aatacgaaag gtaatgcttc aataacgatc
     2521 gataagaaaa tcgtactcat ctgcaaaacc gaatttggaa taaaagaaaa catgagtttc
     2581 ctccttttta tttctagttc attcgctttg caaatatacg cttttattca aaaaaaacaa
     2641 gttgctctgt aaatttttac acaactctta ttttttacca aagcacttca attgactttt
     2701 tctgttattc ccattaaatt aaggtctcat acatatcact tctatcataa aagggagaat
     2761 aaaaatgaaa caaaccacta acttgactga agttcaagac atactgcaac agtcatccgt
     2821 tgccgtcatt tatctttcga tgcctaattg cagtgtttgt cacgctgttc gtccacgact
     2881 agaggaactt ctcacacatt atgatattcc ggcacttcat ttggatgcat ttgaaacacc
     2941 agaagtagct agtaaatttg aagtgctgac tgcgccagtt gtcctgattt ttcatcaagg
     3001 aaaagaagtc tttcggcaag cacggttcat cgactttaaa aaaatccgtt atctactaga
     3061 tgaactgaca gcagaagatg attccctttc ttatgaagaa atcttccgaa caaaataatc
     3121 gttgtccaaa cagaaaaaag cgattgaaca agtgaaagtt ttgccttcat ttgttcaatc
     3181 gctttttatt tttattctgc tgtattttcg gaatgcgcat ttgcgtcaga atgcaagaaa
     3241 tagatcaagg tttgcaattc attcgtcaaa tcaacatttt ggatcaatac atctgacgga
     3301 gcattcaatc gagcagcggt aaagttcaag attcctttga ttcctgcttc cgttaactga
     3361 ttgactactt cttgtgctct ttcagcgggt atcgttaaaa ttgctacttc gatttgttgg
     3421 atcttgattt gttctgacat atcttccatt ggataaacgg ggataccgtc tacgatgcgc
     3481 ccaacgattt cctcattcac atcaaaagca caactgatac gtatgctatt actttgatgg
     3541 aatttgtatt ttagtaaagc acttcccaag ttccctacac cgatcagcgc aacatttgtc
     3601 aattgatcgt cgtttaatgt ctttgcaaaa aagtgcataa gatcttctac atcataacca
     3661 tacccacgtt ttcctaattc gccaaaataa gaaaaatctc gacggatcgt tgcactatct
     3721 acttgaacgg cttcacttaa ttcagttgaa gacacttttt tctttccagc attatttaaa
     3781 atccttaaat agcgataata caaaggcagt cttttagctg tcgcctttgg aataatatga
     3841 tctttcacga aatgacctcc acgtattgtc taattacatc tttgatttct ccatcatagc
     3901 agtatttata ggaaaaaatc aattccattg ctcagatatt gtgaaatttt attctttatt
     3961 catttattta tcatttctgc aaatctcgca gtataatgga atttatggta gactactaat
     4021 tgaaaaatag ttttttattt gaggagaatt cacaaagtag aaaagaggat tccatgatat
     4081 tattacaagc caatcagatt gctcgccatt ttggtgcaga agtcttattt gaaaacatac
     4141 atttagaaat cagtacaggc gctcgtgtcg ctttagttgg cagaaatggc gctggaaaat
     4201 cgactttatt aaagatcatt gctggaattg aagcacctga tgaaggaaag atcgctaaaa
     4261 ataaaacagc gactctcgga tacctcgctc aggacacagg acttgattcc aatgaaacag
     4321 tttggaacga gatgctgaaa gcttttgaac aagttagaca aatggaagag cggatgcgcg
     4381 agctagagat tttcattagc gaaggtacgc cagacacacc tgcttatagt tcttatttaa
     4441 aagaatacga ccggctgcaa cacgattttt cagatcgaaa cggctatggt tatgaaaatg
     4501 aaattcgttc cgtattgcat gggtttaaat tcgattcttc attttatgat cagaaaatcg
     4561 atactttatc aggcggacag cgcacacgtt tagctttagc tcgcatgctt ttgcaacacc
     4621 ctgacatttt gattctagat gaaccaacga accatttaga tatcgagaca ctttcctggc
     4681 ttgagaatta tctgcaaagc tatactggtg cattattgat tgtttcccat gaccgttatt
     4741 ttttagataa agtagtcaat gaagtctatg aattaagcag aagaaaaatg cgccattaca
     4801 aaggtaacta ttcgaaatat ctagatttga aagcagaaca gttagcaagt gaatggaagg
     4861 cttatgaaaa gcagcaaaca gaaatccata aattagaaga cttcgttgct cggaaccttg
     4921 ttcgcgcctc taccacaaaa cgagcgcaaa gccgcagaaa gacattagaa aaaatggacc
     4981 ggctggatcg tcctcaaggg gatgaaaaac aagcaaactt tctattcagt atagaaaagc
     5041 cttctggaaa tgtagtcttg caaatcgaag atgccgctat cggatatgat aatgtcacct
     5101 tatctgaacc aatcaatcta gatatccgtc gtcaagaagc gattgctcta gtaggtccga
     5161 atggtatcgg gaaatcgacg ttgctgaaat caatcattca ccagatccct tttatccgtg
     5221 gaaaagaaac gctagggaca aatgtcacca tcggctatta cgatcaaggt caagctgatc
     5281 tgcatacaaa caagacgatc ctagcagaat tgtgggatga gcatcctacc actccagaaa
     5341 aagagatccg aagtgcttta ggtgctttct tattttctgg cgaagatgta gaaaaaccaa
     5401 tccctctgct aagtggcggt gaaaaagcac gggttgcttt agccaagtta gcgatgaaca
     5461 aagaaaattt tttgattttg gatgaaccaa caaaccattt agatattgac aacaaagaag
     5521 tattagaaaa tgcattgatc gattatgacg gcacactgct ttttgtttcc catgaccgat
     5581 atttcatcaa tcggatcgcg acaaaagtcg tcgaactctc tgaaaatggc agcaaagtct
     5641 atctaggaga ctatgattat tatttagaga aaaagcagga agaagaagaa atcgccgaat
     5701 tgttggcaca agatgagcca gaatctgctc ctgctcccac gaacaaatct gattattacc
     5761 aaagcaaaga acagcaaaag cttatccgta gccttcaacg aaaaattact caggttgaag
     5821 aagaaatggc gcggatcgat gaatggatcg atcagttgga acaagaaatg gtcgcgccag
     5881 aaaacctgga agatcacgta aaactcaatg agctgaacca agatttagaa gctgctagac
     5941 aagatcaaga aacaaaattg acggaatggg aagaactaag cttggaatta gaagaatatg
     6001 aaaattaacg aaaaaaccaa aacagaaacc attcatgctg ttttggtttt tcttttttta
     6061 ttttatcaaa caaaaattcc atatttacct ttttcaccaa taagaagacc aattactagt
     6121 cattctcact aaagcttcta aataatagta atcaccccaa atacaaggtg tatttacccc
     6181 attatttttg ggcttattat aaacagactg tgttaaaaag ccatcttctt cttccgttgt
     6241 agcatatctt tctttcaatt gtcgcataat tttccaaaca gcagactggt ataagttctt
     6301 ttctgttttg tctaatagac ctgattctat taattctaat aatccacaaa ccatgatcac
     6361 catactagat gtatcttttt cttctctgct agaatcatca aagatcaaat cccaataaca
     6421 aatggtatct tctggcaacc gattcataaa ataattggct aatattttgc ttaaatcaaa
     6481 atatttttgt tctttcaaat aacgataact taatgcaaaa ccgtaaatcc cccatgcttg
     6541 gcctctagcc caacaggaat tatcactata accttgatgc gtacttccat atcttggttt
     6601 tccatctttg acatccatat aaaatgtatg ataagttgaa ccatcttctc ttactaaata
     6661 atctgctgca ttttgaatat gagcctgagc agctttttta tatttttgat ttccagtttc
     6721 ttcactcgcc caatacaata atggaagatt catgcaacaa tccatgatca ttctaccttg
     6781 ttgtgaagga tcactaggat ctccccaagc ttgtataact cctgccttct cccaaaaacg
     6841 ctgaagtaat gtttctgcag catcaattga agcttccttc atttttcgat ttcccgttat
     6901 tttataatct gctacggttg ataaagaata caaaaaacca agatcgtgat gattagtgat
     6961 aatttgacag tcaatcctgt ggataaaatc tatatctgct ttttcagctg tttgttgaaa
     7021 taaaggatca cccgtcttct caaataacaa ccaattaatt cctgtccaaa atccttctgt
     7081 ccaaccaata ttgtctctta atggataacg tccttttata ggacactcgc ttgggaattg
     7141 atactgactg ttactgatac gtttgattaa cctctccgtg tgtctgcgac aacgctcgat
     7201 ctcattattc cattcttttt ccattaattc tttataacat tcatttatcc atttttctgg
     7261 atgtattatc tcttcaaaca tcactttatc aagcatttcc ccaccagcct ttagcataat
     7321 tttagtcttt ttttattgtt tttattttgg atagttagcc cggttgtccc atcaaaatca
     7381 attttgggac gattgttcca tacttctaaa ctataatccc gatcttgatg agcaaccact
     7441 cctgttccga gccaatgggt tccttttcca aatgtaccct ctaatccagg aataaaagtg
     7501 ttttcccatg tcatcaagtt tgtattgggc aaacccataa tagctgtgga ttttcgttta
     7561 atagtatcgt cacttaaaca gataatacct gaaaagccag cttcactcac cctatagttt
     7621 tccttgtctt cttctatgat tattccttct ttctcaggag aacattcaat agcaaaccct
     7681 gtctcatata gaacatattc tgtatctaat tctactttgt gtacgcgaat ctgccagcct
     7741 tcaaaaggaa taagccaagt tctgattttt gcctgtaaag taggttccca tgtagaggca
     7801 agtacctctt tcttacatac aatgtctcgt acattatttc gaacgatgaa ctgtccatag
     7861 gttttgatgc tctcattctt aaaactggtc gtgatatatg tgtccgctcg ctgaatccct
     7921 agcattgaat ccattgccat ttctgctttc aattgattcg ctcgcggtat cgaaaaacca
     7981 aattgactag aataagcaaa ttttaaatat ttttcattgg tcaatgtatg gaaatttggc
     8041 cctaattgtc ccgcattcaa aaaaaatgta tgtccattat catgttgtat gagcaaattg
     8101 gcttctgaaa gaagtttagt tccttcacga ataggcatag gcatttcttt agccgtccaa
     8161 tactcatggt cttcaggtag cgccaacaat aaaaaaatct tattcagcca taatggagat
     8221 aacgtactat tatagggttc agtcaatgtc aattgagggt aggtataacc aatattcagg
     8281 ataccttttt catcaaatat ttctttttgc atccaccatc ttatattccg attgactaac
     8341 cccttgattt cacctaattt aaaaggcaaa agatcggccc acaacatagc tgaccaaaag
     8401 cttgaaactg caaaacgata aatcatgctt cgaccaaaag gcacatttgc accatcttcc
     8461 gcaaaaaaat gaatatagtc tctagaaaaa tcaatagctc ggttcttata tatctggcta
     8521 atgggctttt ctggttctag tacgctataa atcaaaccat aataatgaaa tgcaaaagga
     8581 ttataataat cttggcgacc tcttgaactg tctcgatacc agccattttg aagataacaa
     8641 ttttctatat tatcaaatgc ttctttcagt gctgattctt taactggata accaaatttt
     8701 tggataattg ttccgacaat tatcttaaaa aattgccaat taccatctgc acattcatat
     8761 tcaaaaattt gcgagaacca atgaataatc tggctttttt ctcctgtatc taattttttc
     8821 cataataatt gttcatgatg atataacgct aataacattg cgggcatttc taccatacgc
     8881 tggtctcttt catgtatttc tccccaataa tcgggatggg aaggatctgt tccagctttt
     8941 attcctgata gaattaattt caaccatcgt tcatctaatt ggtcaattgc gggccccgct
     9001 ccccataaaa cccgagagaa tccttccatt cctgcaattt tatttccata tcctacccct
     9061 tgatgtatat aatcaatttg tgcgccactt gctcgataaa acggctggag tggttgattt
     9121 atctttttaa ataagtcaaa gtaatcctgt ctggtcctta atagtggtac tggatgtttc
     9181 attgtatttt ctcctctttt tactaccaaa tagctaattc cgactgttct aatcgaagta
     9241 agccttcaat aaaaaaataa tcagaataga tcagtggcac attataagtc agttctctat
     9301 ggtatgcttc tgctccgcct ccaatgatcc catcttcatc catattccaa tttgcatagt
     9361 gcttttctgt cttttggagt atattgatag cagcttcatg atacattttt gatcgtacag
     9421 aggaaacata tcctgctaat tccagaagac cgcatgcagc gcatactcca gcactggtat
     9481 caatattcga atcttttaca gcaaaatcaa tgagaggaat ataaccagtt ttttcgattt
     9541 tctgaataaa atttactgct atcttttctg ctatagctag atagtacgga ttttttgtgt
     9601 aacgataact taacgcgaat ccatataaag cccatgcatt tcctctgctc caacaagaat
     9661 tctcattgta tccttgcccg ccaagtaacc gaatatattt ccctgattct gcatcaaatt
     9721 ctgcgatatg cccggttgat ccatctttcc tcactagata cttagctgtt gtatctgcat
     9781 gctgccgagc aattttagag aacctcggat caccagtttc cctcgaagcc caatacagta
     9841 atggtaaatt catcatacta tcaataatca tccaaccagg tttatggtca ttccatgcta
     9901 ctaaatagtt ccctgaaata ttatagcgtc cagcaagtac cgtagctgca tgtaacccta
     9961 gttgtcgact atgtttgttt tgagactgtt gataatgtgc aatagccgat ggtaagaata
    10021 aaaaacctac atcatgatga acgtctgaaa aatggtacag ggcagattct aataattgtt
    10081 cttgttgtat tgctgcctct ttgaactttt ctcgttttgt cttgtgaaag agctgccata
    10141 aaataccact ccaaaaacca ttggtccacc agtcgattcc tctgtccttt attacgtctg
    10201 tatattttcc gttgtaagaa atatatggaa ttttattccc cattctgtct aattccgcat
    10261 ctatcttttc agaagctaca ttcaaaattt ttcttgacca ttccttcgtc tgttcattca
    10321 tggatatccc gccattcttt taatttttat gaatgtggag ttgctcctag aacgaactag
    10381 atggttaaca ttcgttattt tttttactta tttgcagctc caatgtcatt ctccttaaat
    10441 ttagactgcg cccacacata aatcaagacc attgggatca ctgaagttgc taaaattagc
    10501 ataattgcgc cataattaat tggctcacct gtactagaaa ccgtcatatt tttgataatt
    10561 aagggaaccg tatatttatc tttatctgaa agcatgacta atggaaatag atattgattc
    10621 caacttccca taaagataag taaaccacta gtaataatcg taggtgataa aatcggtaca
    10681 gcgattttcg taaaaatcac ccactcattg gcaccatcga ttctggcagc ctccatcagt
    10741 gctgtaggaa attgactgct atattgtcgc atcataaaca ctgaagttgt actggcaatc
    10801 gaagggatga tcacacctgc ataagtattc gataatccta aacgactcat aatgtaaaat
    10861 aaaggaatca atgtggtaaa aaaaggcacc atcctagata gcataactgt attgaacaac
    10921 agtttattcc ccataaaacg atactttgct aatgcataac ccgccattgt agaactggct
    10981 gtagctaaaa ctgtactgat tacagcaata acaatcgaat tccaaataat caagggataa
    11041 ttatatctcg ccatgatggc ttgccagttt tttaatagca tatccccaaa cttgaaattg
    11101 attccgccac ttaaaatttc ttgattactt tgcgttgaag ctacaatcat atataaatat
    11161 ggcatcaaaa atattaaggc accaaacaca ataatgacgt acgtaaaaat tctgctttgt
    11221 atcttttgtt tagtcatttt tatcacctcc gaatttaaat tgaatagccg ataatataac
    11281 agatacaaac accatcgtcc aggcaatagc agaggctttt ccaaaatcaa ccatttcaaa
    11341 acttgtttta tataaattga ctcctacgac caaagctgaa ttttctggtc cattggaagt
    11401 cccaaagaat atatttggga tattgaaatt acccaagcca ttgatagtgg ctaaaactgt
    11461 ggccattaaa atgattggct ttagcatggg gatcgttaat aaaaagaatt gccgaaattt
    11521 cgatgctcca tctatttcag cactttcgta tatctcttta gaaatccctt gaagaccagc
    11581 taaaaataaa atagtgttat atcctacact aagccaaatc atcgtgatga tcattgatat
    11641 tcgtatccaa aaaggatttg ttaaccactg tacaccgtta attcctagcg cattcaaaaa
    11701 tccattgaat aacccagtcg gattgaaaaa agtcatgaat acggaagcta cagccaccgt
    11761 tgatgtaatg gttggaatat aatagacagt tctaaaaaat ccttttcctt ttaactgtgc
    11821 tgaattcaat aaaacagcaa agtaaatagc taatagcatc gttattggaa ttgatcctgc
    11881 catgataatc aaaacattcg tgtaagattt aataaacaat ggatctcgga agatagctag
    11941 ataattgtca aatccaacaa attcaaatga gtagccaatt ttcataaaac taaaagcaaa
    12001 cgagataatg atgggaatca aaatcgtcac aataaagaaa ataacaaaag gtgtgaccaa
    12061 tccataagca ctttttttat gagacgtatc tgttgctttt ttcttgctgt tctttattgt
    12121 cttgcttggt aacgaagatt gcatcatcgt taagtcctcc taatattttt taatcaccag
    12181 ttaattttac gccgtactta tgagcaaaat tttctgcttc ttttagatat gaattttcaa
    12241 aatcacctgt ttgccaatag ttatatgcag cggtagtcaa ataagacgta gcatctgaat
    12301 aagtggctgg taaaacagta tttgatgtat tttcattgat tttattcaat agttcatagt
    12361 atttttgtcc tccttagtag tcaaactttt tctcagctgc ttctgtttga taggccaatg
    12421 agttagatgt agccacgccc agctccagtg cgttggctaa gcattcttca tctgtcagca
    12481 taaatgtaag aaattgttgt gccacttgtg gattttttgc atctttaggg acataaaaac
    12541 ttgatcctcc tgatactgca ccttttcctg gattttcttt tgaaaaagga atcagtggag
    12601 ccatgcgcca tttgtcagat gcatcaggaa aattcaaaga catattcgta gcataccagc
    12661 cgccagcaac aaacattgca gattcttgaa atgctttctc agcatcttgt gcaccgtaga
    12721 aatgtacaat atctgagtcg atcatttctt tgattaactt tgcagcatgt ttcgcttctt
    12781 tagtattcag acggatattt ccttcttcat caagcaaaga ttgattctgc tgggctagca
    12841 tgttgataaa caattcttgg tccccagatt ctggcaaggc aattccaaaa ctaccagttt
    12901 tttcttttaa tgcttttgcc acctggatat attcttccca agaactgatt tcttcaaaat
    12961 ctacacctat ttcagcaaat ttttttggtt gataataaca aagtgctggg gtaaaatcac
    13021 tagcccaagg aataatcgtt tcattactcg tttgttgacg caaaatattc aatttttgat
    13081 cataaaactg atcgccatat ttgtcataaa atccatattc atcagcacta taaaaagcat
    13141 cttgaaattt ttccagcaca cctgctgctg aagtgtctgc catggagata atatccggca
    13201 tatcttcgtg tgttaccaat tttggggtga tcatttgtac tgttgctgtt cccgaagcta
    13261 cattcacaat ttttaagtca atatttgctc cagttttgcg gttatagatt tttgcagcat
    13321 tttcaacata gtttgtcagt ttgccgtttg cgacatatac ttctaatgta gtggcttttt
    13381 ttatcattgg atctgcgatg tttgctgtta cttcttcgtt agtttttcca catgcactta
    13441 agctgaataa tagggtaatg tttagcaaag ctatcaccaa tttgtatctt tttttcatgt
    13501 ctatcccttc tttcttttca agttcttata taaaacgctt tcacttatgt atattaacgg
    13561 aattagtaac acaaatattt atataaaaaa atattttact tgtcgttttg gcttttcatt
    13621 tttgttataa tagtcattaa ttataaatag aaagcttttt atataaaaag agaaaggaaa
    13681 gtggttcttt tgttaacaaa caaccaatcc tccaatcttg tcaaaaaaac actaacctat
    13741 tccatagata cccatcttca tttatctgct gctccctatt ttcgactact aataaatgaa
    13801 agcgctaatt tatatgctga atttgcacat aaatctatag aactagataa tccttccctt
    13861 ttattcattg ataaacagca agaagtccaa atcttttcta ctcaaaagga aacacaaact
    13921 tttatcatta tccagtttta ttttgatgag caattattag cacatattac gggacaatct
    13981 ttttctatag atcaagaaat aacatctatc attacttttc tgaaaaatga acagttcaaa
    14041 attgattatc taaaacatag taagccagct atttcctatg atcaggaaat caattctttt
    14101 ttcatactat gccaacagat gagcattcat ttgctttcct tattagttac ttattgtctg
    14161 caaaaaattc aattgtcttc cttaccaagg cattatcaac aggaacttgc tttattgaca
    14221 ataccttcaa atgagaaagt cattcctaaa aaaaagcata ttctttattc agatgaatta
    14281 gttgatccaa tcatacgata tttagaagaa catctttcag aaaatttttt gcttgaacaa
    14341 ttagcccaaa ttttttttat cagtccagcc agcttaaaaa aacattttag aaaagtgaat
    14401 ggaaaaagtg ttattagcta ttttaaagaa ctaaaaatgg aaaaagcaaa acgtctcgtg
    14461 ctagaaaatg aattaaccta tacagaaatt gcagatatat taggatttca ttcgatccat
    14521 catttttctt cagcttttaa aaaatataca gggttatccc cttcaaagta ttatctatcc
    14581 ttaaaataga aaaaaatttt taggttttat ccacaaatta tttctctata ttttctgtct
    14641 attcaaaata aacaataatt ttccggcaca attatttgct gttttttacc ataacatagt
    14701 acgatcaacg tagaaaagga gatgttttta tgaaagttgt tgtcattggc ggtgtagctg
    14761 gcggtccttc atttgccact cgtttccgtc gattgaatga agcgcacgaa atcattatct
    14821 atgaacgcgg agaaaatatt tcttatgcaa gttgtgcttt gccttattat ttaggaggtg
    14881 tgatcactga ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa acaaaaaaca
    14941 acatcgatgt ctttactaaa cacgaagtaa cagcaatcga tccttctact aagcgattaa
    15001 cagttaaaga cctatccaca aatgaagaaa taaaaacaga ttacgataag ttgatcatct
    15061 cttctggtgc tagaccagat tatccggata ttcccggagt ttttgaagca gaaaacggct
    15121 ttgtcctccg tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa gaaaaaaatc
    15181 cacaacatgt cgtcattctt ggtgcaggtg ttatgggtct ggaattagct gagaatttca
    15241 agcatcgcgg cttagacgtg actttaatcg atcaattgcc acaagtcgct ttcccttatg
    15301 atccagaaat tgctaattta gtttatgaca aattgctgaa agaaggatta gccgttcatt
    15361 tagaaacaag agttactgag atccgtgata aaggtcgaga aatagtatta tcagatggct
    15421 ccgtcctttc tgctgatatg ctaatttttg ctgttggtgt ttctccgaag aatgaagtgg
    15481 tgaaagcaac cggcatacaa ttatcagata caggtcagat cattgttgat gaccagttac
    15541 aaaccaatct gccggacatc tatgcgattg gcgatattat cgaaacgact agtgtagtga
    15601 ctggtcagcc gatccaaagt atgctttcca gtgcggccaa tcgtcaagga catatgctgg
    15661 cagatatttt aaatggtacg cctatgcgct atcgcggata tattggtgca ggtgtcgcaa
    15721 aaatctttga tcatacagca agttatgcgg gaatgacaga acatgaatta aaagcatcag
    15781 gcataacaaa ttataaaact gtttttatca ctccctttga ccatgcctat ttctatccag
    15841 aagctacaag attaaatcta aagctgattt ttgatgcaga tagcggtcgt attttaggtg
    15901 gacaagcatt tggagaaaaa ggtgtcgata aacggatggg agaactttct gtagcaatca
    15961 ccggaaactt gacagtcttt gatttgcctg atttggagtt gccttactct ccaccgtatt
    16021 ctactacccg tgacccgttg aatatagctg gttatgtcgc aatcaatcaa atgacgaata
    16081 tcgtagaaac gatcaaagca agtgatatac ccgaaaacga tttgaaagaa gcgttctttt
    16141 tagatatacg tgaacctaat aaagcacctt ctggcagtat ctcggcaaca aaaaatattc
    16201 caatgaatga gttacgagat cgaatcaatg aaatcccaaa agataaaaaa gtttacatta
    16261 ctttcagaag aggattgaat acttatactt ctgcccgaat cttggcaggt ttgggtatca
    16321 aagcgatttt gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg tctaggagtt
    16381 atttttactt gaggagtaaa aatgaaaaag tatatagggt tgttggttgg tatgtatata
    16441 gaatagaaga taaatgtgaa gaaattgtgc attatttctt cacatttatt aggttatagt
    16501 ttaagaaagt ttaaacattg cgcaatttaa ttattttagc tgtttctttt cataaaaaaa
    16561 catgttgtga ctctgagaca aatcatagtc catagtgatg tctctagagt aactacgcct
    16621 aatattacga ggcatcatat taatctatca gcactagtac ctaaactagc atccctttcg
    16681 aatatttttt cttatataat aaagtttgct ttcactcctc taatctcacc aaatcgactg
    16741 gcttgcgttg tgtgatacta ggaactgatt ggtttagaac gacttttcta ttgtaatcat
    16801 atttttcgcc atcctcgttg tcttctaatc cttcaatgaa tgatccctaa tgtgtgtatt
    16861 aaataattta atttttttgt tcctatttat ttaa
//
LOCUS       DAYYCQ010000063        16769 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000063 DAYYCQ010000000
VERSION     DAYYCQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.063"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..303)
                     /locus_tag="ACHAYF_002028"
     CDS             complement(<1..303)
                     /locus_tag="ACHAYF_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8579460.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKS"
     gene            complement(305..502)
                     /locus_tag="ACHAYF_002029"
     CDS             complement(305..502)
                     /locus_tag="ACHAYF_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8579461.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(896..2623)
                     /locus_tag="ACHAYF_002030"
     CDS             complement(896..2623)
                     /locus_tag="ACHAYF_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8579462.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2616..3809)
                     /locus_tag="ACHAYF_002031"
     CDS             complement(2616..3809)
                     /locus_tag="ACHAYF_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8579463.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3996..4640)
                     /locus_tag="ACHAYF_002032"
     CDS             complement(3996..4640)
                     /locus_tag="ACHAYF_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8579464.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4734..4868)
                     /locus_tag="ACHAYF_002033"
     CDS             complement(4734..4868)
                     /locus_tag="ACHAYF_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8579465.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4870..7002)
                     /gene="feoB"
                     /locus_tag="ACHAYF_002034"
     CDS             complement(4870..7002)
                     /gene="feoB"
                     /locus_tag="ACHAYF_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8579466.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6999..7472)
                     /locus_tag="ACHAYF_002035"
     CDS             complement(6999..7472)
                     /locus_tag="ACHAYF_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8579467.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7873..8097
                     /gene="nrdH"
                     /locus_tag="ACHAYF_002036"
     CDS             7873..8097
                     /gene="nrdH"
                     /locus_tag="ACHAYF_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8579468.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8256..10415
                     /gene="nrdE"
                     /locus_tag="ACHAYF_002037"
     CDS             8256..10415
                     /gene="nrdE"
                     /locus_tag="ACHAYF_002037"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8579469.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10465..11430
                     /gene="nrdF"
                     /locus_tag="ACHAYF_002038"
     CDS             10465..11430
                     /gene="nrdF"
                     /locus_tag="ACHAYF_002038"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8579470.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11519..12658)
                     /locus_tag="ACHAYF_002039"
     CDS             complement(11519..12658)
                     /locus_tag="ACHAYF_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8579471.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12967..13680)
                     /locus_tag="ACHAYF_002040"
     CDS             complement(12967..13680)
                     /locus_tag="ACHAYF_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8579472.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13934..14635)
                     /gene="nagB"
                     /locus_tag="ACHAYF_002041"
     CDS             complement(13934..14635)
                     /gene="nagB"
                     /locus_tag="ACHAYF_002041"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8579473.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14869..16401
                     /locus_tag="ACHAYF_002042"
     CDS             14869..16401
                     /locus_tag="ACHAYF_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8579474.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16592..>16769)
                     /locus_tag="ACHAYF_002043"
     CDS             complement(16592..>16769)
                     /locus_tag="ACHAYF_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8579475.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5206 a         3452 c         2887 g         5224 t
ORIGIN      
        1 agatttatct ccataatctg ctcgatataa accatttatt ccagcagttc ttccaatttc
       61 actcaaattt cttattaatt cctcttgata ttcagtgaat cttattttat tagtataagc
      121 agtaaaatat agataattca ccaatctatt tattaaagga ctgggatcta attcatattt
      181 ttgtttttct aattttaaat ataccttttg taaaacctca agaatatcag aatatttagg
      241 atctttactt atagatatat cattatataa ttcatgaaca atttgcttag catttttttt
      301 cattttagca cttccctgga attgctccac ctaaaaaacc acctatagac attcctgcaa
      361 tacaagtagt tgccttggcc caatcgaccg tacatttatt tttagtgcaa tacactccat
      421 ttccataata ttttccacta tgagtggtac ccccatagat aagttgtgtc tctttaatag
      481 acaaaatttt taaatgtttc ataatctaat cacccctaaa ttaatgtttt tagactatta
      541 ttctaacatt cattaaattt caaaaaacaa ttccttcttg aaacgtagca aaataattcc
      601 tgaataagaa tttaatcact tttaagtata taaactatgt atattcttta atattttttg
      661 agaatgcact tcgtacataa taaactttaa tcacattagt ctttcatacc tacgatcgat
      721 ctctttgact cgcacccata aattcataat agtttgtcgg cgtctttgaa cgtttcgaag
      781 atgagctacg cattctcttt ttttatagac aaaaaagata gagacgaaca tggagcttcc
      841 aaatttggat atttgatcca attttggaag ctccatttat ttcgtgtctg tctttttatt
      901 ttttccagct ttcgactaat tcttggttct ctttgatcca gtcttttgct gcttgctgtg
      961 gatcttttcc attgttgatt tccaacataa ctttttccat atctttttct gaccaatgga
     1021 aattgtctaa tactttgtaa gcttctggct ggtcttcttt caatcctttt cgaaccatcg
     1081 tatggatctg ctcttcgctt cccattccgt tttcaggatc ttctaagtat ttcagatcat
     1141 atttggcaaa catccaatga ggtgtccatc ctgtaactac aattggttcg tgttttttga
     1201 tcgcttgacc tagtgcaacg gtcattgctc ctgaagaaga tgtctctact ttccagtctt
     1261 tcaagttgtc atatttttgg atcgtgtttt cagcagccgt aactacacca gctcctggct
     1321 cgattcctat gattttctta cctgcctgat cggttaactc atcgatcgaa tttacatcca
     1381 tgtaggctgg cactgctaaa ccaacttttg ctcctgtcaa attggcacct aggtcttcga
     1441 cttgatcttt atattgtgca tattgtgaac catgagtttt aggtagccaa gctgaaacca
     1501 tggcatctga ctcgccattt gaaacagatt tccacatgat tgaattatct aatggcgtca
     1561 tcgttacatc atagcccatt tgtttcaata cttcaccgac tacatttgtt gaagcaactt
     1621 ctgtatccca ttcaacataa gataagttga tacgttttgt ttctttggca gatgaaaaac
     1681 tccctatacc aatcgcacct aagatcacta ctactacagc accaatgatc agtccttgac
     1741 gttttttact tttctgtgtt gttgctcctg cagctttttt attgtttggc tggttcaaat
     1801 gctgtgtaaa acggtcaatg atgattgcta agataactaa tgcaaccccg ttgacaaatc
     1861 cattcccgac ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac
     1921 cgatcataga ggcaataacg accattgaaa gtgacagcat tgttgtttga ttgatccctg
     1981 ccatgatcgt acttttagct aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc
     2041 tcccgaatga atcagaagct tctactaatt ctttaggaac ttgtcgaatc cctaaattcg
     2101 taaaacgaac ggttggtggc agagcaaaga tgactgaggc aaatacccct ggaaccatcc
     2161 cgataccgaa aaatgcaact gctggaatca aataaacgaa tcctggcatt gtctgcataa
     2221 agtcaaggat cggtttgata atactttggg ctttactgct ttttgccatc aagatcccta
     2281 atggtacccc aatgatgatc gagatcacac tagataaaag aacaagtgtg acagtattca
     2341 tcaaatcatt ccatagacct tggttataaa taaaccatag accgattaat gtgaacaacg
     2401 taagtcctgg acgtttctta gaaataaaga aagctgctac cgtcaataac aagataaaca
     2461 aaggagctgg aatcactaac agtaaatttg taatccccga catcactgcc tgtccaatcg
     2521 tttgtaaaaa tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta
     2581 cccagtttgc cactggtaat tgatagttca atagattatt catgttcact cacctcgctt
     2641 gtttctgcta atgcttcaat cacactacct cggatgacga caccttttaa ttttccatcc
     2701 tctacaactg caagtggagt cggtgagtca taaataatat tgaaaatatc acttaccacc
     2761 atgtctttcc caatcaccgt cacattttca tctaggaagt cgatcaatgg acgtttttgc
     2821 ttcctagctt ctaacgcttg atctgcagtg atgatccctt tcaggtgtct ctttctgtca
     2881 acagccatca gcatgctgac ttcttcatta cgcatccttg tcaatgcaac gtttggtcca
     2941 tcagattcga tattcgtcgt caatgcaggt accatgatgt tttgagccgt aagcactttg
     3001 gaacgatcga cttcttccac aaattcacgt acataatcat ttgctggatt ggtcaatatt
     3061 tcttcacctg ttccgatctg catgatttcg ccgtctttca tcaaggcgat tctgtcccca
     3121 atccgtaaag cttcattcaa atcatgggta atgaaaataa tcgttttttg aacattggct
     3181 tgcaggtcca gtaattcgtc ttgcatttct cgccggatca gcggatcaag agccgagaac
     3241 gcttcgtcca tcagcaagat ctcaggatca tttgctaaag ctctggctag gccaacccgt
     3301 tgttgcatcc caccagataa ctgactagga tattggtctt taaatgaaag caaactagaa
     3361 ttttctaatg ctttttctgc tttttcttgt cgttcttctt taggtacacc acggacttct
     3421 aacccgtatt ctgtattttc caagatcgtt cgatgtggaa acagaccgaa gttttggaaa
     3481 accatattga ttttatgacg acggacttct cgcaattctt ctttgcttaa cttcgataca
     3541 tcttctccat caatatagat actgcccgaa gttggctcaa tcagacgatt caatagacgg
     3601 atcaatgtgg atttcccact cccggaaagc cccatgatca cgaagatttc tccttctttt
     3661 acgtcaaaat tcacgtcata cacaccaacc gttgcaccgg ttttctttaa aatctccgtt
     3721 ttcggctggt gatcattcat catatctaaa gcggcttgtg tctttttacc aaatatcttt
     3781 gttaaatgtt ttacttctac ttttaccaaa caaattctcc ttttttatgt aaattcattt
     3841 ttctttctga aaaaagtgac cacactatgt taacatcatg tagtcacttt gtcaaaaatg
     3901 aaagaaattt gcttgtccaa actgaaaaaa acttggctaa atctttgtaa ataaaaggtt
     3961 tagccaagtt aatatttttt taatatttca cacaatcatt ctgcaagctg atgctccggg
     4021 taaaaaaagt ttgtcatacg ctgtagtgta tattcatttc caataaaatg aacgatgtcc
     4081 cctgccgtaa atttagcata ggggccaggt gaaacaagca gttcttcatt atgagaaatt
     4141 gcgataactg tggcgccagt cgcctgccag atattcaact cgctaattga acggtccaga
     4201 tgcattgctt tttctgataa agataattca aacggaagta aaggattcat cttatgtatt
     4261 cgcttcgttt gattgaccag ctgatccaat aattgagaaa aatgattcag ctcatcttgt
     4321 tgcttctcca cacttgccat gatttctgct tttgtatctt ggatggattg tacatcttga
     4381 tattgcaata ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc acttccatgc
     4441 cgtacttcca tgattccaag atctaccagc acattgatag ccttgcgggt agtctcagga
     4501 gaagcattgt acgtactggc aagtgttgag cgggcatgga tcttctcacc cactttatat
     4561 cggttttcaa cgatacgttc ggctaaatca acagcaatct gctggtagcg cgggcggacg
     4621 atttttttct ttccagacat tttctttcct ctttcttttt ttactttccc tcatcttact
     4681 atgctctagc aaaataagca aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc
     4741 ttgatctgtt ccttttttac tgggcaagct gtttgacagt catcacaatt cttaccggat
     4801 tttaaacgag tatagacaat tgcgcctgca cttccaaaga ttaacacact taataagaat
     4861 gtcgccatcc tattcctctc cttctaatga caatatagta taaaactggt tcttggattt
     4921 tggctgacgg aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc
     4981 taacgctacc tgtccaccct ccaataagtg tcccaactga taaatcacaa aactaactaa
     5041 ataagctaaa ccgcattgat agcctatggc gatccaagtc catttcttgg cattcatttc
     5101 ccgatggata gccccaattg ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa
     5161 tgaataagct gctgcgggag tatatgtgtg ttgtagcact tgccagattt ctctgccatt
     5221 ttcggatact tccgcatgac caaacaaaat accaaatgtg ccaataatat tttctttagc
     5281 gaccaatcct gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa
     5341 aacaactgcc aggattcgcc cgaaaaaggc taagatactt tggtcttcag aaacaggttg
     5401 agcataaaaa ttataagaag agctgaacca gatcacgata ctagaaacaa agataatcgt
     5461 tcctgctttt ttaacaaaag acttgctgcg gtcataggtt tgtcttagga ccgctgacca
     5521 ttgaggaaaa tgatatgcgg gcaattccat gataaaagga gctgtatctc cagcaaacaa
     5581 acgtgttttt ttcaagctga ttccagagaa aataattgca gtcataccaa gaaaataggc
     5641 agaaggagct acccaagatt gatgaggaaa aaaggcccct gctactaagg caataatcgg
     5701 caactttgca gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc
     5761 gttttcaatc gtgcgactgg ccatgacgcc tggcactcca catcctgtag caatcagcat
     5821 aggaatgaag gattttcctg aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc
     5881 gatcctcgcc atatatccgc agtcttccag aaagctcaag cataaaaaca atataatcaa
     5941 ttgcggtaga aatcctaaca ctgctcccac gcctgcgatg atgccgttca agatcagctc
     6001 ctgcatccat cccgctactt gccattcagc taacaatctg ccaacatgtt ctggtaacca
     6061 tgtaccaaac aatgtgtcat tgatccaatc tgtccccatt gttcctactg tttgaatagc
     6121 cagataatag atcagccaca tcacaaatgc gaagatcggc aaggctaacc aacgattcgt
     6181 tgcaatctga tcgattttgt cactcatcga taattggaaa gaatcatttg acacaacaca
     6241 gagtgtacat agttgggtaa taaattcata acgttcattg accagaatcg tctcactatc
     6301 gtcgtccaat agtttctctg ttaactggat cagttgctcg atctcttttt gctgagaaac
     6361 tgctatctcg taatcctctc ttaccttctg atctcgttca aaatatttca ttgcatacca
     6421 gcgtttcttg ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa
     6481 acgtggatca tatgtacagg gaacaggaat agaaggccgc ttttttgcag actcctgaat
     6541 cttttcgatt agtttttcga ttccttgctt tttcaatacg ctgattggta aaactggtac
     6601 accaagacca taggaaagct tttctagatt gatttttttt ccttcttttt tgatgacatc
     6661 catcatattc agaccaatga tcataggaat gtctaaatcc aataactgcg tagttaaata
     6721 aagatttcgt tctaaattgg tagcatcaat aatgttgatg atcagttcag gctgttcctg
     6781 aaccaaataa tctcttgcaa tcacttcttc tggtgtataa ggagacaaag aatagatgcc
     6841 gggtaggtcc tgaatatgaa tgttcggatt ttttttcaac gatccttctt ttttttctac
     6901 tgttacgccc ggccaattcc ctacatattg attcgaacca gtcaagagat tgaacaagct
     6961 agttttcccg ctgtttggat ttccgactaa cgcgatattc attctttttc ctccctttgt
     7021 atcaagatta gttcggcctc tgactttcgt aacgtcagtt cataccctcg cactgtcagt
     7081 tcgattggat ctcccagagg ggctagtttt cggataacta tttttacatt tcgcgtcaat
     7141 cccatatcca ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac gacgatccca
     7201 gtctctcctg cttttaattg atcaagagaa gtccagcttt cagaggaagc agtcttttta
     7261 ttgacaaaga tttgacctag tatttcccga cttactgcca ctcgatgact ttttaccagt
     7321 aaaatggcat tgttgcctga taaattgacg acgacgattt tacttccggc aacgatcccg
     7381 atattttgca aatggcgttt tgttttttct tctgtttcga ttcgttcgac ttgataaacg
     7441 aaattctgct ttgcctgcgc taaggtctgc atattgattc ctctcttgtg aaataaatca
     7501 tgtattaaaa tcattcatta tatgttctaa ttttcttacg tgttacgcaa ataaatgaga
     7561 atcattctca catagaatac tctcggtaat tgagaatgtc aattatactt gtgatattat
     7621 gagcgaatct ttagtattga tagataaaaa tgagaaaaaa atatttcttc atagttttcc
     7681 acaatttttt ctctattaaa tttgctattt tcccacacga tacttgtttt cagctctttt
     7741 ttcaaaaaca caaaacttag tattccacag tttccacaac acaatatgta gtgtttgttg
     7801 tttgactttt ttactaagag ttttatacta tctattatag ataataagaa tgtgttagag
     7861 gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg
     7921 gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag
     7981 cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct
     8041 gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat
     8101 gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt
     8161 gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag
     8221 catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg
     8281 tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga
     8341 ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga
     8401 cttttccatc tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag
     8461 aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc
     8521 aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga
     8581 aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc
     8641 tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc
     8701 aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat
     8761 tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca
     8821 actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc
     8881 gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca
     8941 tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg
     9001 gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag
     9061 aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata
     9121 aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg
     9181 aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag
     9241 ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct
     9301 caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg
     9361 agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc
     9421 aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaaca gatatctcat
     9481 gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg
     9541 tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac
     9601 caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac
     9661 acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa
     9721 atatttactt catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg
     9781 aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg
     9841 aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag
     9901 gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg
     9961 gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg
    10021 atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa
    10081 tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt
    10141 ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaacggca caacaacacg
    10201 tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg
    10261 aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt
    10321 tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa
    10381 tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca
    10441 tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat
    10501 gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca attttggcta
    10561 gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa
    10621 aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa
    10681 agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac
    10741 aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg
    10801 aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa
    10861 aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt
    10921 gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat
    10981 ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa
    11041 tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa
    11101 gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac
    11161 gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca
    11221 ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca
    11281 gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac
    11341 catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa
    11401 gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa
    11461 aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt
    11521 attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc
    11581 ttctcaataa atagattttc cggatattta atattatcgt cttaatcact gcatagacag
    11641 gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga
    11701 tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc
    11761 catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga
    11821 taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa
    11881 tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca
    11941 ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg
    12001 cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg
    12061 gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa
    12121 taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga
    12181 cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg
    12241 atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta
    12301 accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt
    12361 ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa
    12421 acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg
    12481 gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaatt gccgaaagca
    12541 ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg
    12601 tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac
    12661 atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga
    12721 gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta
    12781 cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa
    12841 ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag
    12901 gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca
    12961 gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa
    13021 ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt
    13081 gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt
    13141 tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat
    13201 ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc
    13261 aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa
    13321 tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac
    13381 gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc
    13441 ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc
    13501 actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg
    13561 cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa
    13621 tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat
    13681 ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt
    13741 tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata
    13801 aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct
    13861 atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc
    13921 ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt
    13981 cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt
    14041 tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa
    14101 ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc
    14161 gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg
    14221 aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat
    14281 cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg
    14341 cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca
    14401 tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta
    14461 cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg
    14521 gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga
    14581 tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga
    14641 ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt tttacaataa
    14701 ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta
    14761 ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc
    14821 actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg
    14881 acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg
    14941 caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca
    15001 ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa
    15061 aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa
    15121 gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt
    15181 taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa
    15241 acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc
    15301 gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa
    15361 cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat
    15421 gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat
    15481 tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc
    15541 catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca
    15601 ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa
    15661 caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg
    15721 gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca
    15781 agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca
    15841 actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc
    15901 tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa
    15961 acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa
    16021 atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat
    16081 ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca
    16141 gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact
    16201 aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg
    16261 acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca
    16321 ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc
    16381 attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa
    16441 aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat
    16501 tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga
    16561 tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc
    16621 ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg
    16681 ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac
    16741 ctccaataaa gtacataaac ataaataca
//
LOCUS       DAYYCQ010000064        15538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000064 DAYYCQ010000000
VERSION     DAYYCQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.064"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..889
                     /locus_tag="ACHAYF_002044"
     CDS             242..889
                     /locus_tag="ACHAYF_002044"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8579476.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(1214..1360)
                     /locus_tag="ACHAYF_002045"
                     /pseudo
     CDS             complement(1214..1360)
                     /locus_tag="ACHAYF_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1599..2291
                     /locus_tag="ACHAYF_002046"
     CDS             1599..2291
                     /locus_tag="ACHAYF_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8579477.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(2573..2645)
                     /locus_tag="ACHAYF_002047"
     tRNA            complement(2573..2645)
                     /locus_tag="ACHAYF_002047"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2610..2612),aa:Lys,seq:ctt)
     gene            3026..3595
                     /locus_tag="ACHAYF_002048"
     CDS             3026..3595
                     /locus_tag="ACHAYF_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8579478.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            3582..4442
                     /locus_tag="ACHAYF_002049"
     CDS             3582..4442
                     /locus_tag="ACHAYF_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8579479.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            4632..5336
                     /gene="yycF"
                     /locus_tag="ACHAYF_002050"
     CDS             4632..5336
                     /gene="yycF"
                     /locus_tag="ACHAYF_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8579480.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5341..7176
                     /gene="walK"
                     /locus_tag="ACHAYF_002051"
     CDS             5341..7176
                     /gene="walK"
                     /locus_tag="ACHAYF_002051"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8579481.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            7173..8489
                     /gene="yycH"
                     /locus_tag="ACHAYF_002052"
     CDS             7173..8489
                     /gene="yycH"
                     /locus_tag="ACHAYF_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8579482.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8490..9359
                     /locus_tag="ACHAYF_002053"
     CDS             8490..9359
                     /locus_tag="ACHAYF_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8579483.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9414..10223
                     /locus_tag="ACHAYF_002054"
     CDS             9414..10223
                     /locus_tag="ACHAYF_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8579484.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10326..10955)
                     /locus_tag="ACHAYF_002055"
     CDS             complement(10326..10955)
                     /locus_tag="ACHAYF_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8579485.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            11125..12417
                     /locus_tag="ACHAYF_002056"
     CDS             11125..12417
                     /locus_tag="ACHAYF_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8579486.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12493..13155)
                     /locus_tag="ACHAYF_002057"
     CDS             complement(12493..13155)
                     /locus_tag="ACHAYF_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8579487.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13380..13664
                     /gene="groES"
                     /locus_tag="ACHAYF_002058"
     CDS             13380..13664
                     /gene="groES"
                     /locus_tag="ACHAYF_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8579488.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            13715..15340
                     /gene="groL"
                     /locus_tag="ACHAYF_002059"
     CDS             13715..15340
                     /gene="groL"
                     /locus_tag="ACHAYF_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8579489.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         5320 a         2561 c         3264 g         4393 t
ORIGIN      
        1 tttgagggga agtacataat gcaaatttgt ccataactta ttgattatct tgtaaaaatg
       61 cggtaattta atagttagtg agaagaggtg cttaattata gttaaaaaag aggaaataga
      121 agcagcatgt aaccaagcaa atgttaggtt tattggagaa gctttatctg gatatcctaa
      181 gtataaatac ttacacaact ggttacaaag taaaagtttt tatcttaata aagaaagatg
      241 catgcctaat aaaaattata gggtttataa aagaggaact attgtatatg ttgattttgg
      301 ggtgaacgtg ggttatgagt tatctggtaa tcattttgct attgttttaa ataataaaga
      361 taattcgaaa aatggtgttg tatgtgtagt accaatctct tcaaaaaaga aaagtaatta
      421 tgtatctgta ggaaaaatat tagaagttgc tagtattaaa caatttataa gccaagctga
      481 taaattaaaa gagaaactta ggataattat tttactttca ttaaatagag gcatcattga
      541 tgaaagcgat ttgaatagtt ttattaaaga tattgaagct aaaggtgaaa agctttcagt
      601 ggaagaagta gaaaaacgag ctgtaaaaca taatttggtt tgcgagacaa gggaacaagt
      661 aattaaagaa ataaaatcat tatctgaaga tatggtaaaa tatcagaagg tgtttaattg
      721 ctacaagaaa tatacacaag aatcctatgt aatgccactt aatatccaaa cgattagtaa
      781 aaatagaatt agaagaatta acaagtttga tccttctgga aatatgaagg cacctggaaa
      841 tgtcctcaat gaagtagatg aaagtttaaa aaaatatttt acaaagtaaa cgatattttt
      901 gttgacattt ttctgttcgt tagttaccat gtagttatag aaggacgtaa gcgcctattt
      961 ttaatgtaca catcgggacg tatgcgccca catagaaaca tagttgagag acatctatat
     1021 ggtgtctctt tttttattaa tatgcaaaaa agtctagttt tttcaataat atttatgtaa
     1081 taataaatta gccaccacaa caaagaatga aatccattat tatctagtct agtccattct
     1141 ttttgtttgc agtatttgtg atggctttcc gtacccttag ctcagttggt cagagcagac
     1201 ggctcataac cgtccggtcg tacttctcgt aatccaggaa ggccgtcagc tccgtccgca
     1261 gtagcgtgtt tacggccagc tccagatggg agcggaataa ctcgttcaaa tcacctttgg
     1321 tggctagagt ctgcaggagt tctgtaataa aatcgttcat agggaagtcc tcttttctgt
     1381 gaatgggtgt ggtaacttta ttctacagaa gggacttcct tttttctatt tacacaaaat
     1441 attttatact ctcaaatgtt tgtatgcagt tacatctgac tttattcgaa ttaaggaaaa
     1501 gatttataaa aataaaataa aaaaatatat atttaaattg acttattgtg aaatcattaa
     1561 ctataattag ttcagtttat gttaaggaga atgatgatat gaataagaaa attgtagtaa
     1621 gtggattaat tataggtgga ttagtgttag cagggatcgc agggaaaaac tttgccgatg
     1681 acattttgtt atggggtgga gaagataata ttgaacaaat caatcataat ttggagtcat
     1741 tagataaagc actagaaaat aaagagcaaa atattagtag tttaaattca cgattatctt
     1801 caaataatca agaacttgag caagcaaagg ctaacgttga agaatataaa caaaaagtgt
     1861 tgactttgga aaatgataaa aatcaattaa ctatagagaa aacaaattta gagaatcaat
     1921 tatcaggaaa aaatgctgaa ttgcaaggta aacaaaatga aatcaatgct aaaattgatg
     1981 aaataaatca aaaaatagct gaaataaatc aattaaacac tgattggaat aatcgtttac
     2041 aaacagaaca aaataaattt aatgatgctc aaaatactgt taatagttta aatcaacaaa
     2101 ttaatcaatt gtcaaatgaa aaaaatacta ttaaaaacca attgcaaagt actgaacaaa
     2161 aactagaaaa agcgcaatca gaaaacagta atttgaataa ttatattgaa aaactggaaa
     2221 aagccaaaag cgatgtagaa gacacagcaa acagatcaca agaaatcgtg actgaacata
     2281 caggaaaata gatgcatttt ttagtttaaa tttctttaac aaaatagtat tttgttgcta
     2341 aataattttt atttgaatat tctacaatga tatctcccct caaaatggag gggaagtaca
     2401 tactgtgata atctacaggt ttattgactg attaggatgt tattaaaaaa taaaatacta
     2461 aaatgcatac ccgaatttca tacccgaaaa ataaaaaatg ccaccacgta cactcttttc
     2521 gaaactacct aagtagccaa gaatgtactg gtgacgtggg tttggcaccc aatgggccgt
     2581 gaggggctcg aacccgcgac ccgctgatta agagtcagct gctctaccaa ctgagctaac
     2641 ggcccgtaca aaaagtactt aataatagta gcactaagaa ttttaaaatg caagactaca
     2701 tttaaagaaa atcagaaaaa tctttacttc cttataggaa atcattcata gattattcac
     2761 atttgctcgc tattatataa gcataccaac aaacaaagtt cttgcaagag aacaaaccct
     2821 aacacttttt tcatttttta ctcctttccc gccgtctccc cggcgggtct cctgttctta
     2881 acgataaaag acaagaaggc ttagccttct tgttttttgt tttcatggaa tcccgctaat
     2941 caaatcacct agttaaagca actagattga atgtttatca taagtctgat atcatgttta
     3001 tgaaatgaaa aaaggagcgg ttatgatgga aaaaatgcgt cagcaattaa tggagtgggg
     3061 gaaggaacta gaaacattcc gtctgcctca ctgggaagaa ctccctgact tagaacttta
     3121 tatggaccaa gtcagaacgt tagttgatcg ttatctttca cctgtgatac aaggagaaaa
     3181 gcatccattg cttacctctt ctatggtaaa caattacgtt aaactaggtt tgataccagc
     3241 accagttaaa aaaagatata acaaagaaca cgtagcattt ttacttgcaa tcactacgtt
     3301 gaaacaagta ttgactattc ctgaaatcaa agaagggata ttgttccaag gaaaaacagt
     3361 aggaattcgt gaagcctata atctattttg cgatgaacaa gaagcagctg tgtggatggt
     3421 cagccaatta gcacaaggta aatctcatcc gcaaaaattc gaccaggcaa cccctgtaga
     3481 atatattgct gtaagagcgg caactttatc gtttgcaatg aaacttcttg cggaaaaaac
     3541 gattgttcta gaaaccgaat atttaaaaga ggagaaaaca aatgaaaaac aataaaatcg
     3601 caattttagt agattcaggt acggatgtgc cagaagaatt gataaaaaaa tacgagatgt
     3661 atatgatccc attgaaaatt atttataaag atcaggtata tacagataaa attgacatta
     3721 cctctgaaga gatctatcaa cgtttgccag aggaaatccc tggtacatct ttgccggatg
     3781 gtgagtcgat cacagaaatc tttgagcaaa tcaaaaaaga cggttatgaa aaggtacttg
     3841 cggtaacaat ttccagtgga ttgagcggga catataatgt ggttcgttta attgcacaac
     3901 agcaagaaga attggaagca tatgtactag atacgaaaaa tattggtatt ggtgcaggtt
     3961 tttctgcgat ccaagcagca aaatggctgg aagaaggagt agaatgggat caactgatca
     4021 gtaatctgaa tgagttagtc gaaagaacaa aagttttctt taatgttgcg acattggaat
     4081 atcttcaaaa aggtggacgg atcggtttag ttgcttctat tgtaggaacc gctttaaagc
     4141 ttaacccaat catctcatgt aatgaagaag ggatttatta cacagtagga aaagctaggg
     4201 gacgtaaaaa aagtttggat aaaacggttg cttacgtcaa agaaaaaatc ggtaatgcaa
     4261 aggtcttcaa cttagctgtt gcccatgggg atgcgaaaga agaagcaatt gaaatggaag
     4321 cgagacttaa aagagagttc ccacaagcaa accaaatcta ctttggccag atttctccgg
     4381 cattagtagt ccacactggt ccgggattat tgggtgtcgg tgtacaagtt ttagaaggat
     4441 aaatcaaaaa aaggaaacac tgaggtgaaa atcagtgttt tcctttttta ttgaagttag
     4501 aacaaaaaat aataaagtat tttgcacttt cattttaatt ttctcttttg ttttataggc
     4561 aaagatgaaa caaaagcaga aatacggtat aatgagaagg tatgacaata atatataaga
     4621 ggtggctctt tatgaaaaaa gttttagttg tcgatgatga gaagccgatt tcggatatcg
     4681 tcaaattcaa tttagccaaa gaaggctatg atgtttatac tgcatatgat ggggaagaag
     4741 ccttggaaaa agttgctgag gtagaacctg atttgatttt acttgatttg atgctaccta
     4801 aaatggacgg cttagaagtt gctcgtgagg tacgcaaaac ttatgatatg ccgatcatta
     4861 tggtaacagc caaagattct gaaattgata aagtcctagg cttagagtta ggcgccgatg
     4921 attacgtgac caagccattt tccaaccgag aattagttgc acgtgtcaaa gcaaatcttc
     4981 gtcgcggtgc cacggctgcg aaggaaccag aagaggcagc acctgcggaa ttgacgattg
     5041 gtgatttgac gatccatcca gaagcttaca tggtgacgaa acgaggagaa acaatcgagt
     5101 taacacatcg tgaattcgaa ctactctttt acttggcgaa gcaccttgga caagtgatga
     5161 caagagaaca tttattacaa acggtttggg gttatgatta ttttggagat gtacgaacag
     5221 tggatgtaac tgtacgtcgt ttgcgtgaaa agatcgaaga taatccaagc catccaaatt
     5281 atttagtgac acgtcgtggt gtcggttact atttgcgtaa cccagaacag gagtaattgg
     5341 atgaaaagaa aagtccgctt ttttcgatca gtcaatttta agatcgctat tacctttatt
     5401 ctgattttat tgatctcgat cgaaattatt ggtgcctact ttatacgtgg tttagaacgt
     5461 tccacgatca atacatttat taaagatatg aatcaaacgg tggaatcatt agcaacaaca
     5521 atcagcccgg aattgaatcg aaaagataat gcagatgatg aagaggtaaa tgcgaatatc
     5581 aaaagattta ttgaaaacag tgcgactagc gatatcatcg aaatacgggt cgttgacgaa
     5641 aaagggatca tccgaggaac aaccgatgtc aatgaacaaa gcgctgtggg aaagaaaaat
     5701 gattatgttg atatcaatga tttcacgact aaacgttatg ttgctttaga taacgacgat
     5761 aaacgggtga atatcaatgt tcaaccgatc ctgtccccga ccggcgatgc cgttatcggg
     5821 gctctttacg tcaaaagtaa tatcgaacaa aaatattcgg aaatcaataa cacagcagtc
     5881 attttcttta ctgcctcact aattgcggca tttatttcaa tgatcgtatc tgttttagtt
     5941 gctcgttcga ttacacagcc gatcggtgaa atgagagaac aagccattcg aatcgctaga
     6001 ggagactaca gtcggaaagt caaagtgcat ggacaagatg aacttggaca gctggctgat
     6061 accttcaatc agttagctga acgtattgaa gaagctcaag atacaatgga agctgaacgc
     6121 aatcgtttgg atagcgtgct atcccatatg acagacggcg tcattgcgac agatcgcagg
     6181 ggtaaagtca ttacgatcaa tgatatggct gtttccttgc tagatatcaa aaacgaggaa
     6241 gcaatcggac aatcgatttt gacactttta gatatcgaag aagaatatac gttgcggaaa
     6301 ttattagaag atccaaacga aatggtactt gaccgttcaa ccagtacatt ggaaagtgat
     6361 cagatgatct tgcgagtaga cttttctatg atccgtcgcg aatcaggttt tatcagcgga
     6421 ttagtagctg tcttgcatga tgtgacagaa caagaaaaaa cagaacggga acgtcgtgaa
     6481 ttcgtctcta atgtttccca tgaacttcgt acaccgttga caagtatgcg tagttatatc
     6541 gaagcattga gcgaaggtgc gtggaaagac gaggaagtag caccaaattt tttgaaggta
     6601 acgctagatg aaacagaccg aatgattcga atgatcaatg acctcttaaa tctatctcgc
     6661 atggatacag gtaataccca gcttcaatta gaatatgtca atttcaacga gttagtgaat
     6721 tttgttcttg accggtttga tatgatggtg ggaaatcagg aaaaaaatta taagatccgt
     6781 cgtgaattta cgcaaagaga tctttgggtc gaagtagata cagataaaat catccaagta
     6841 gtcgataata tcatgaataa cgcgatcaag tattcaccag atgggggaac gattacttgt
     6901 cgcttattag aaacgcataa taacgtggtt ttaagtatca ccgatcaagg attggggatt
     6961 cctaaaaaag atctgaaccg agtgttcgag cggttctatc gagtagataa agcacgtgca
     7021 agagcacaag gtgggacagg acttggatta gctatttcta gagaagtgat taaggcgcat
     7081 cgaggcgcta tttgggcaga gagcaaggaa ggaaaagggt cgacttttta tatctcgctg
     7141 ccatatgaac cttatgagga ggaatggtgg gaatgagaat ctctgaaaaa gtcgttcgta
     7201 taggactcat tttgatggtt gcgttaagca tatacttctc ttatgcaatc tggctgagcc
     7261 cagcaggaaa aagttcaatc aacgtagatg agacaaacac tcaaatgatc gagtctcaaa
     7321 gcaatcgaaa aacttccgaa gtctttcttc cgcttcatgt tacatggcac cattcgggaa
     7381 tgatccaaga aaccaacagt gaaaatctcg tgtaccgatt gcaattgatc atcgataaag
     7441 caagatttgg aaagctttct gaagtagttt caggagacaa atcacagttt gaacaggtga
     7501 aaaatctgaa ctctggtttt gagttggcct ataatgcgcc ttttttgcta agagaataca
     7561 aggaagcatt tgatttagat ttagatgtgt cgaacttggc aaaagaaagc gaaaatatgt
     7621 attttactag aatacaattc gatcaaggaa ataaaaaaat ccgatttttg aattattcga
     7681 accatctcgt atatgaagca aatatttcgt tgaattggac agatatcaat aaagaattaa
     7741 cagcaaaaga cacaaaatgg acagaaatga caacagaacc atcgacgatc gacacgcaat
     7801 atgctacgaa acattcgatc aaactgaaaa agtatagtta tatcctgtca cagcaaccct
     7861 atacactttt tagaaatgct tttttccaga aaccagatag cgtgaaaaat aatgatgaaa
     7921 gcaatgaatt gattttttac ggtgggaatg aaacaatgtc tatccattcg gaaacgcaag
     7981 tagttgattt tcgtggagag ctgccagaga aaaaagaaaa tgctagtgta ttttctgaaa
     8041 gtttccccta tattagcaaa ttaggcagtt ctttagggaa cctcagatat tttgaccgca
     8101 gtgacaatac gatcgattat cggatattcg tggaaggctt cccggttttt gggacagaca
     8161 gcagaggaca gattggtgtg gaagttggcg gtgaaacgaa tggttctgtg caagtgaata
     8221 tccaaaccag tttgaatacg attcaagtgc cgataccttc agacgaagaa gtagaacttg
     8281 caagtacgaa tgaaatcatc cagcagttaa aagctaaggg agcagatagc aacaagattg
     8341 gttcagtgat cgttggctat acatggcaaa atgtcaaaga aacgaatcgt gtcgttgatc
     8401 tcttacctga atggtatgtg aaatataaag ataactggta ttcagctaac gaccttctgg
     8461 aaaaattacc tggattggag gcgaattaat tggatttcaa aaaaatcgaa tggatatttt
     8521 tcgtcgcttt tttagggttg aatgtgtttt tattcagcag ttatcatgac gcggtatacc
     8581 aagaaaacaa tgtcattcgc tctaatcaga ctgaatcaat ccaacaacgt ctggctagcg
     8641 acgatatcac ttacacgggg aagatttcgt ctgagaacaa gcaaggctat tatcttagcg
     8701 cggagcagac caatcttagc acttcattag cgaattatcg caaggctggt aaaagcggct
     8761 tgctggcaag tggtgtgttt ttagacggtg atgttttgac gaaatcttta tccagttcag
     8821 atgctgaaaa gaacgtgatc gatccagata aaattagtag ttccttggaa gattttttat
     8881 ctaacaaaga caatatttta ttcggtaatg attatgtgta cacgaaaaac ttttctcatt
     8941 tagaagaaga accactagaa atccaagcaa gccaagagta tgaaggcatc ccattcaatg
     9001 atgatacagc aaaaatcgtt ctttctttag aaaaaaacga taatgattat agcatcacga
     9061 aatacatgca gacgcattta tctgatatcg agcagctgcg cgaaaagacg gaactgtata
     9121 cggaagagga agcaatcaat acattgtaca tcaataacaa aataccaaga ggatcaaaaa
     9181 ttttatggcg tcagctagct tattcctgta ttctgaaagt ccgggaaaaa aatgtgtatg
     9241 ttcctgtttg gcatgtagcg atcgaaacaa gcgataaagt ggtccaaatt gagagtgtca
     9301 acgctttcag caatacgatc gtgacaaaca atactgtacc aaaagtggaa gatcaataaa
     9361 aaagcgagta taatgaaaaa agtttacaaa gtaagaaggg aagtaggagt caaatggctg
     9421 aagaaaaacc agcttttaaa atcagtatcc tagcaagtgg aagttcgggt aattcacttt
     9481 atatcgaaag tgaaaaaaaa cggcttttgg tcgatgcagg acttagcggt aaaaaaatca
     9541 cttccttgat ggctcaaatt gatcggacac cggaccaatt agatggaata cttgtgacgc
     9601 atgaacatcg agatcatata catggcgtgg gtgtcttggc tcgtaagtat catttggatg
     9661 tttttgccaa tcagcagact tgggaagcga tggaaccact gattggaaat gtaccagtgg
     9721 aacaaaaaca tctatttgag atggggaaag tcagaacatt cggcgatatc gatattgaga
     9781 gtttcggcgt ctcccatgac gcagcggctc cgcaatttta tcgtttccat aaagaaaatc
     9841 ggtcattcgt catgttgacg gacaccggat attgcagtga ttatatgcga ggtgtcattg
     9901 aaaatgccga tggttatttg atggaaagta atcatgatct tgagatgtta cgcatgggac
     9961 cttatccgtg gaatttaaaa cagcgtattt taggagatag aggacatctt tccaacgaag
    10021 acggtgcact tgtcatgact gatgtgatcg gtaatcggac caaacgaatc tatcttggcc
    10081 atcttagcaa ggaaaacaat atgaaagaac ttgctcattt aacaatggaa aatgttttga
    10141 aagaaaaaga ttttggcgtt ggacatgatt ttgaaatcta tgatacagat ccagatgaag
    10201 caactgagct attttcgatt taaacagttc aatcaataaa aatataaaaa gcagaagata
    10261 aaatcaatca gggttggttt tatcttctat ttttattttt ttagcatatt aaaaaaataa
    10321 ccgtattatc gactgactac actgatgtgc tgcttttcat gtttctcatc cagtatctca
    10381 tctactaatg caatcgcgta atctgccatg ctgatcgtac tgtcgccagc agcgtttctt
    10441 tgtaaataat catcacttaa caaataagta cctgtacgtt tgccatgtgg atcaaataaa
    10501 gaagctggac tgatataagt ccaatgtaga tttttagatt gtttcaattt taacaatcct
    10561 tcgtacatat tgaaagctgt tggatagaag ggtgcatttt taggtacttg gtcaatcata
    10621 cgagtcgtct tttctgtatc taagaataac gaagaggcac ccccaacgac aattaagcgt
    10681 gtatcggttc cttcaagcat ctcaatcaaa tgggacaaag aagtttgatg catttcttct
    10741 tttcctttgg gagcgttgaa agcatcaacg acaacatcaa aagatgcaag atcagtagtt
    10801 gtcaactcaa ataaatcctt ttgtataaac ggaacatctg atggcaattt ttctggatga
    10861 cgcacgattg ccgtcacgtc taaatctctg gacaatgctt cgtctaaaat aaatgaacct
    10921 gcatggccag tagctccaat gagtgcaatt ttcataaaaa acctcctttt ttattcctgt
    10981 ccattgtaag cagttgctga atattcgtca aagaatccga tttttgaaaa gctcagtgcg
    11041 cattcctttc atggttcgtg atcattgggt atagttaaat taagaaaact gattgataaa
    11101 aatagaagtt gaaggagata agaaatgaaa gagtggttag atcgtgcctt tttaccaatg
    11161 ataacatctg ttgtgaatct tagaccctta actgctttgc gtaatggaat gacttacacg
    11221 atcccaataa ttattgttgg ctcgctgttt ttcttatttg ctcattttcc tgtggattac
    11281 atcgctcagt ggatagacaa aacaggattt tctatgtatt ttacacaagt ataccaggct
    11341 acatttgatg tgatagcctt ttggatcgtt tttgccattg catacgctta tgcaaaagaa
    11401 gaagacatcg atcgcttacc agtcggcata acagccttgt ctgcttatct actgcttatt
    11461 cagccactaa atactcagat agatactgtc tggacaggtg ctgaagggat actaggagca
    11521 gttacaactg ggctcatcat cggatggggc tatgcacgtt tagtaagact tgcgaaaggg
    11581 tggaagcaaa gagagaagat acctgaaaat gtttggcagt cttttacttc attgattcca
    11641 atcattgtta tagtcacttt tacgcttatc ggttcagcgg tttttcaaca gttgttcaga
    11701 atgactgttg ttcaaggagt atggacatat gtccagcttc ccattcaatc gttcatagac
    11761 acgttgtttg gtgtgctttt attaggtttt tttgttccgt ttttatggtt atttggtctt
    11821 catggatctg cgatggtcaa tgggttggtt agtcccgtct tgcaagcgaa ttcattagcg
    11881 aatgcagaaa tcttagcctc aggacaagag ttgacggtgg caaatggcgg acatatcgtt
    11941 actcagcaat ttctcgatca gttcatgacg gttacgggtg ctggattgac gttaggggct
    12001 gtatttttta tgacggtttt tgcgaaatcg aagaaataca gagaattagg aaatcttgct
    12061 cttttaccag cctttttcaa catcaatgaa tcaattattt tttccacacc gatcgtgatg
    12121 aacccgatga tggctgttcc ttttattttc gcaccggtcg tttcaggatt gattacctat
    12181 tccgctttat attttggatt cgttccttta tttacagcag tccaagtccc ttggacaaca
    12241 ccgccgatcc tctcgggttt cttagtaggc ggagtgcctg cagcgatttt acaaggtatc
    12301 gtgttgtcaa ttagtttttt catttatttc ccatttttga aaaaaataga ttctgcaaac
    12361 tgcaaaaagg aaagacagac aaaaaatgat ggagcagcag aaaagatgaa cgactaaaaa
    12421 acaaaaatac tcctgtcgct ttagattggg aaaatcacca atctaggatg acaggagtat
    12481 ttatagtcat gtttatacta gccctttttg gacagctaat tgaacaagaa caactaatgt
    12541 gttcattcct acgtgtgtga tcatcgatgt ccagatccga ccagttgctt tgtactctag
    12601 gctgaagaag aaacctagga aaaagtagat tagcagatga ccatcattat gagcaaacgc
    12661 aaacagcaaa gagctgatca ctgctccgac ccaaaaattt gaatatcgac tgatgatccc
    12721 cagtatactg cggcggaata cgaactcttc catgatgggt ccagcaacag ttgtcgccaa
    12781 aataaaagcg ggtgcttcca tgatcatctg gatgatattt tgtgtattct cggaaggggt
    12841 tgctttgcca aacaatacag attctaacat gacagcgatg ctttgcagaa tcaatgcgac
    12901 aaagattcca accaatccat aaataacaat ctttggagca gaagcttttt ttaccgctga
    12961 ttcgatcgct aagggttcac gttgcttaaa ataaaaaata gctaggacaa cggcgcctag
    13021 tatatacatg actgcagttg ctgtgatcaa ttcagacgta gattttacaa gtcctatcga
    13081 agctaggatc aagggtgaga taaaaattag accatagcaa aaaatgctga ggaagctata
    13141 tttttttaaa gacatgtaat tcctcctttt aagggataac aagcatgcaa ggcaagctta
    13201 tatcgattga tttcaattat atcataattt caataagcag ctgtcagaat aaaatttctt
    13261 ttctttgatt taaaagcttg caaaaaagag ggagaatgag tattattaaa aatgtaggtt
    13321 agcactcgaa ggttaagagt gctaacagat taaaaaaaga gttatcggag ggatttatcg
    13381 tgttaaaacc attaggtgat cgcgtcatta tcgaagtcgc aaaagaagaa gaaaaaactg
    13441 tcggaggaat cgttttagct tctgctgcta aagagaaacc ccaaactggg acagttgttg
    13501 cagtcggtga aggccgtttg ttagaaaacg gtgaaaaagt tcctgccgct gtaaaagcag
    13561 gcgaccaagt tatgttcgaa aaatacgccg gtacagaagt aaaatacgaa ggaaaagaat
    13621 atctgatcgt cgcaggaaaa gatatcatgg cgattgttga gtaaatttgg aagataaaaa
    13681 aataatcaaa aataatcaat gaggtgaatc aatcatggca aaagaattga aatttgcaga
    13741 agacgcacgc gcagctatgt tacgcggggt agataaatta gcagatacag tgaaagtaac
    13801 attaggacct aaaggccgaa atgtcgtact agaaaaatct tacggctctc cattgatcac
    13861 aaatgatggt gtgacgatcg caaaagaaat cgaattagaa gatcattttg aaaacatggg
    13921 tgcaaaactt gtatcagaag tagcatcaaa aacaaatgat attgcaggag acggtacaac
    13981 gacagcaact gttctgacac aagcaatcgt tcgcgaaggt ttaaaaaacg taactgcagg
    14041 tgctaatcct ctaggcattc gtcgcgggat cgaattggca acaaaagcag cagtcgaaga
    14101 attgcataat atctcaactg ttgttgattc aaaagaagcc attgcacaag tagcagctgt
    14161 ttcttcgggt tctgataaag ttggacattt gattgctgat gcaatggaaa aagttggaaa
    14221 cgacggtgtc atcacaatcg aagaatcaaa agggatcgaa acagaactag atgttgtaga
    14281 aggtatgcag tttgatcgtg gttacttatc acaatatatg gtaacagata acgacaaaat
    14341 ggaagctgtt ctagaaaacc catatatctt gatcacagac aaaaaaatct ctaacatcca
    14401 agatattctg ccattgttag aacaaatctt gcaacaatca cgtccattgt tgattattgc
    14461 tgacgatgta gatggtgaag cattgccaac tcttgtattg aacaaaatcc gtggaacatt
    14521 caacgtagta gctgtaaaag ctcctggttt cggcgatcgt cgtaaagcaa tgcttgaaga
    14581 tatcgctatt ttgacaggtg gtacagtaat tactgacgat ctaggtttag aattgaaaga
    14641 tgtaacaatc gaaaacttag gaaatgcttc taaagttgtt gtagataaag acaatacaac
    14701 gatcgtagaa ggttctggag aaaaagaagc aatcgaagct cgtgtgcaat tgatcaaaaa
    14761 ccaaattgct gaaacaactt ctgattttga ccgcgaaaaa ttacaagaac gcttagctaa
    14821 attagcaggt ggtgtagctg ttgttaaagt tggtgcagct actgaaacag aattgaaaga
    14881 attaaaatta cgcattgaag atgcattgaa tgctacacgt gccgcagtag aagaaggaat
    14941 ggtctcaggt ggtggtacag ctctagtgaa cgttatcagc aaagtttctg ctgtagaagc
    15001 agaaggagac gtggcaacag gtatcaagat cgttgttcgt gctttagaag aaccaattcg
    15061 tcaaatcgct gaaaacgctg gttatgaagg atcagtgatt gttgataaat tgaagaacgt
    15121 ggaactaggc actggtttca atgctgctac tggagaatgg gtaaacatgg ttgaagcagg
    15181 tatcgtagac ccaactaaag taacacgttc tgccttgcaa aatgccgctt ccgtttctgc
    15241 cttgttatta acgacagaag cagtcgttgc agataaacca gaaccagcag cgccagcagc
    15301 tcctgcaatg gatccatcaa tgatgggcgg tatgatgtaa gaaaaagcaa cttgcttatc
    15361 tacaactgat cattagtgaa agacagataa aaagaggctg gaacagaagc gtttaactcc
    15421 aagaaataag aagaaattca cggaaattgc ttttcaaatt tttgtgaatt tcagcttatt
    15481 tccgaaggag ttgcttctgc ttccgctgtt tatacgtgtt tagagcgtga gacaaaag
//
LOCUS       DAYYCQ010000065        15492 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000065 DAYYCQ010000000
VERSION     DAYYCQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.065"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..454
                     /locus_tag="ACHAYF_002060"
     CDS             104..454
                     /locus_tag="ACHAYF_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8579490.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            553..1083
                     /locus_tag="ACHAYF_002061"
     CDS             553..1083
                     /locus_tag="ACHAYF_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8579491.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1237..2388
                     /locus_tag="ACHAYF_002062"
     CDS             1237..2388
                     /locus_tag="ACHAYF_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8579492.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2497..4536)
                     /locus_tag="ACHAYF_002063"
     CDS             complement(2497..4536)
                     /locus_tag="ACHAYF_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8579493.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDMREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(4594..5151)
                     /locus_tag="ACHAYF_002064"
                     /pseudo
     CDS             complement(4594..5151)
                     /locus_tag="ACHAYF_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(5299..5465)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(5690..5896)
                     /locus_tag="ACHAYF_002065"
     CDS             complement(5690..5896)
                     /locus_tag="ACHAYF_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579494.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5912..6525)
                     /locus_tag="ACHAYF_002066"
                     /pseudo
     CDS             complement(5912..6525)
                     /locus_tag="ACHAYF_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(6672..7355)
                     /locus_tag="ACHAYF_002067"
     CDS             complement(6672..7355)
                     /locus_tag="ACHAYF_002067"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8579495.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7464..7922)
                     /locus_tag="ACHAYF_002068"
     CDS             complement(7464..7922)
                     /locus_tag="ACHAYF_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8579496.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8006..9769)
                     /locus_tag="ACHAYF_002069"
     CDS             complement(8006..9769)
                     /locus_tag="ACHAYF_002069"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8579497.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9990..11450)
                     /locus_tag="ACHAYF_002070"
     CDS             complement(9990..11450)
                     /locus_tag="ACHAYF_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579498.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11658..12584)
                     /gene="glsA"
                     /locus_tag="ACHAYF_002071"
     CDS             complement(11658..12584)
                     /gene="glsA"
                     /locus_tag="ACHAYF_002071"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8579499.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12607..14034)
                     /locus_tag="ACHAYF_002072"
     CDS             complement(12607..14034)
                     /locus_tag="ACHAYF_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8579500.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14065..15279)
                     /locus_tag="ACHAYF_002073"
     CDS             complement(14065..15279)
                     /locus_tag="ACHAYF_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8579501.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4423 a         3095 c         2662 g         5312 t
ORIGIN      
        1 tttgtcacaa tcctttttgt ttttttgaat aatcgctttt tcttctcttt ttcttatata
       61 ctataagtag taaataattt gaaaaaaaga aaggtgactg attatggcgc aaaatcgtat
      121 ttttacgaca agttttgcaa gcgtttatcc tatgtatgta gcaaaagcgg agagaaaaga
      181 tcgtataaaa gaggaagtag atgaaatcat ttgttggctg acgggttatt cccaaacaga
      241 actagaatat caactacaaa aacaagtgac tttcgaacaa ttttttgcgg aagcccctgt
      301 ccccaatcct tcacgttcct tgattacagg tgttgtttgt ggtgtacgag tagaagacat
      361 cgaggataac ttgatgaaag aaattcgtta tttagataaa atgatcgatg agctagcaaa
      421 aggcaaaaaa atggaaaaga tcctcagaag ttgatttttc cagagatatt cttctccttc
      481 gctcattttg taacatttaa tttggtaaaa tgactagttc acgttaaaat aagtcaaagg
      541 aggttttctt atatgtttga gacgattaaa caaaatattc aaaaatacgc cttattaaga
      601 gctgctgttt atattatttc tgggattgcg attgtgctaa acccttctgc tgtttttcat
      661 tttatcggtt atctgatcac cgcctatctt gttttgcttg ggttgttgaa tctttttgag
      721 gcttaccgtt ttaaaaggaa aacaggttct tggagtatcg gtctgttcag tggtcttttt
      781 ttcctgatct tagctctgat cgtctatgct tttgcacctg caatcgtttc tatcctgccg
      841 attcttcttg gattagctgt tatcatcaac agcttgttcc agttgttctt cagcatgaat
      901 acaaaagcta aaagttggag tgtttatagt agtattctgt tgttgattgg cggattcgtc
      961 ttattgttca atccattcaa aagtttgatg cttctattcc aaatattcgg ctttattttg
     1021 attttcatgg gcatccagga gattatcaat ttcatcagaa taagaaaaat gactatacgt
     1081 taatcatttt tagttattaa aatagaccgt ataaaaacca acaatccaaa aaaatctttt
     1141 ggattgttgg tttttttgtg agtatatttt cctatttttt caataatgta tgttatgcta
     1201 agttcgggct atattttata caaaggaatg atgagaatgg aatttattgg tattttatgc
     1261 ctgatattga tttcaacaac gattggctca catatctcgc ggagattagg tatcccagca
     1321 gtgattggac aattacttgt tggtgttttg ctaggccaag ctggattgaa ttgggtcaaa
     1381 cctgatattc tagtccatga cttttcggag attggtgtgt tcttattaat gtttttagct
     1441 ggtctggaaa gtgatttatc tcttttgaaa aaatatttcc gacctggtat gttcgttgct
     1501 ttgcttggga tcatcttccc ggttgttttt ggctggttga caggcaaagc attccaagtt
     1561 tcgggaaatg aagcgatttt ctttgggatt attcttgcgg caacttctgt cagtatttca
     1621 gtagaagtat taaaagaact aaacgtagtg aatacaaaag aaggatcaac aattttgggt
     1681 gcttctgtgg tagatgacat tttagtcgtt ttagttctaa gtttcagcct atcttttcta
     1741 acgggggaag cttctagcaa tgcttcttta ccgcttgttt tgattgaaca gctaatttat
     1801 tttgttttta ttttcttatt agtcaaatgg attgcgcctt tcctactttc atttgcagaa
     1861 aaagtgtacg caaattcttc tattatcatc gtatcgctag ttatttgttt aggaatgtct
     1921 tacgtagcgg atcttgttgg gcttagctct gtcattggtg ctttttttgc tgggatagct
     1981 gtcagtcaga caaaggttaa agaagaagta taccacaatg tagaggcttt aggatatgca
     2041 gtatttatac cagtattttt tgtcagtgta ggattagaag ttgatttttc tgactttagt
     2101 gaacaactac tctttatttc tatattgacg cttgtagcga ttttgaccaa attgcttgga
     2161 ggttattttg gtgctcagat tgccggtttt tctcaacata gttcattgat ggtcggtgct
     2221 ggtatgattt cgcgtggaga aatggctttg atcattttac aaattggaca gcaaagtcaa
     2281 ttgatcgaac cacagtacta ttcaccttta gttatcgtga ttttattaag tacacttatt
     2341 tctccacttg ttctcaaata ttttacaaaa aaagtttatg aagtgtgagt aaatcaaaaa
     2401 gaagttgtga aagagacatc gtgaaaaccg tttatttgaa aaaaggactg tgacgataac
     2461 gccacagtcc ttttactgta cttaaaaagc aattttttat tgataatttt ggttgaggta
     2521 ttgcaatagt tccgatgctc gtttggttgc tgaatcatca tcatctttat tttccaacat
     2581 gctgaccatc atataacctt ggttatcaag attgaaagca aacaagaaac tgttctcttg
     2641 cccctttaca tcctgttttt ctttgatttc cgctgtacca gttttcgctg ccaacggaat
     2701 ccctaaagca gaaagagaat gtgctgtacc atttacatct tgcacaactt ctctcatatc
     2761 tggcacgatc gtttgtactg ctgtttcgct gatgacattc ttcttatctt ttgtctcttt
     2821 atctgcaatc aatttaggat agacaagtgt gccattgttg gtaaaaacag aatacattgc
     2881 tgcttgctgg ataggagtaa ttagaagttc gccctgtcca tatccagtat cagctagcaa
     2941 gatatctgag ttaaagctat cttcattaga aatttgtgct ggattcatac taattggcaa
     3001 atcaaggtct tcaccaaaaa tgaatttatc taagcctgta cgaaattttt tctcacccat
     3061 tttcaacgtt tcttgtgccg catatatatt atcggaatag atcaaagcag tttttaagtc
     3121 tacttgtgat acatcactac tgacacgcgt tgttttaaaa gatccccaag aactgtcttt
     3181 ttgccattta agcccgttga tcgtcaacac ttcatttgga tcgatagtgc cgttgtcgag
     3241 accgattgct gctgtaatta ttttaaacgt agatccagga gcataacctg tcgcaaatcg
     3301 gctgatgaat ggatgttcag gattttcttc ataagctttg taatcttctt gtgagatccc
     3361 gtttgtcatt ttgtttggat catagcttgg agagctagca agcgcaagaa gatcaccggt
     3421 ttttggcgtt gtcgcaacag ttgatccagc ttttcctcct agactgtcaa aagctgtttt
     3481 ttgtgccttc gcatcgattg tcaatttgat atctttgcca ttttgaactt catgttcgat
     3541 cagaactttt ttctctacac cgtctgcatc tgtaatgctt aatttctcac ctgttgtccc
     3601 acgaagatcc ttatcaaaag ccatctccaa acctgagcga ccgatttttc cattactgct
     3661 taattctgga ttcttatcaa tatcttctgc tgtgatatct cccacgtaac caatcagctg
     3721 agcagctgct tcacccaaag gataatatct gccgtctact tcttgaatgg tagctccagc
     3781 tggaagttct ggcgttgctc catcaatgat tttcaatggg acaaagtaat cgggttgtac
     3841 ccagctttgt gaaatcgcct gattgatagc atcttctgtt aagtcgaagg aggaagcaat
     3901 cgctttgata ttggctgttt tttcgcctcc atctccaagt ttacttggaa cgactcctaa
     3961 ttgttttagt ttgcctgttg ttgctaatgg ttcaccattt cgatctataa tattccctct
     4021 tgctgcttct tgtgtggtca ggcttacttt gtcatttcct tccatttctg gaaaaaccaa
     4081 gttgggttgc cagttgatcg tggtttgacc atcatttctg tctaatgttc ctttataaga
     4141 aagatctttc aattcaccta atgaggtatt catctttgct ttatatgaaa aactgtaagt
     4201 agaatcgtct tttttatcta cttttagatt cgatatctga agtcctttga catcagcagc
     4261 accgtatata ttctggtatt gatctaagat ctctttttca gataatgcac ttttatttgc
     4321 cgcctttttt gatgtcattt ctgcagcttt gttataatct cccttgttca aagcttggac
     4381 aaattgctca actgtctttt ctccagcttc tactgcttgg gtttcttgat agtgctggta
     4441 gtaaaaataa ccgccacccg cagctgctat taagatcact gcgccggcaa tataagcgcc
     4501 tgttcgattt ttgccgtgct tgtcacttct tttcattttt tcctccttct cttgcttgtc
     4561 attcctttat ttcactattt atacctgttt attctaacat atgtctttga ggaaatccat
     4621 accacaatcc agaagcacac tttctggaag gttcgtttgg atactaccaa gtaatttccc
     4681 aaccgctttg ggtagcttga atattgaagt tacgtctttc agctgctgag agatagcttt
     4741 gatcacttgc tgctgccttc tgatcctccc gaagtctcct tctttgtctt cccgaaatcg
     4801 agcatactgg agtaaagtat taccatccat cacttgctct cctttgttta tatatacgcc
     4861 atctaaatct agagattttt ctgcatcgat cttgactcca ccaggaaaca gttcattaac
     4921 gcaatcgata aaatcttgaa atgtgatact tgcataatag ggagcttcaa atttgaaatt
     4981 ttcctttaat gtttgattta ataaatcaac tcccccataa gagtaagctg catttatctt
     5041 attcattccg taacctggaa tatcgacata tgaatctctc atgatagaga tcagttttgc
     5101 ttgttgtgtt ttctggttgt attgtgcaac cagaagtgaa tccgagcgac ctccaccaat
     5161 cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
     5221 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
     5281 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
     5341 gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt tacccattgg
     5401 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
     5461 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
     5521 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaat
     5581 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataattttt
     5641 tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt tatttgttaa
     5701 agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag
     5761 taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca
     5821 gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat
     5881 agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt
     5941 ctcctattgc tgcataatca tttcctgcta acggcttaca gcattcaatt ttcttggatc
     6001 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
     6061 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
     6121 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
     6181 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
     6241 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
     6301 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
     6361 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
     6421 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
     6481 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
     6541 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
     6601 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
     6661 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     6721 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     6781 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     6841 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     6901 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     6961 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     7021 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     7081 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     7141 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     7201 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     7261 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     7321 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     7381 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     7441 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     7501 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     7561 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     7621 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     7681 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     7741 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     7801 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     7861 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     7921 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     7981 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     8041 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     8101 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     8161 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     8221 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     8281 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     8341 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     8401 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     8461 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     8521 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     8581 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     8641 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     8701 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     8761 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     8821 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     8881 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     8941 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     9001 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     9061 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     9121 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     9181 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     9241 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     9301 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     9361 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     9421 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     9481 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     9541 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     9601 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     9661 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     9721 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     9781 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     9841 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     9901 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     9961 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
    10021 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
    10081 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
    10141 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
    10201 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
    10261 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
    10321 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
    10381 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
    10441 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
    10501 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
    10561 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
    10621 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
    10681 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
    10741 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
    10801 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
    10861 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
    10921 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
    10981 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
    11041 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
    11101 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
    11161 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
    11221 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
    11281 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
    11341 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
    11401 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
    11461 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
    11521 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
    11581 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
    11641 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
    11701 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
    11761 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
    11821 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
    11881 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
    11941 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
    12001 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
    12061 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
    12121 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
    12181 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
    12241 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
    12301 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
    12361 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
    12421 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
    12481 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
    12541 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
    12601 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
    12661 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
    12721 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
    12781 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
    12841 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
    12901 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
    12961 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
    13021 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
    13081 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
    13141 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
    13201 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
    13261 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
    13321 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
    13381 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
    13441 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
    13501 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
    13561 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
    13621 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
    13681 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
    13741 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
    13801 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
    13861 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
    13921 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
    13981 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
    14041 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
    14101 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
    14161 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
    14221 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
    14281 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
    14341 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
    14401 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
    14461 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
    14521 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
    14581 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
    14641 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
    14701 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
    14761 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
    14821 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
    14881 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
    14941 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
    15001 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
    15061 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
    15121 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
    15181 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
    15241 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
    15301 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
    15361 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
    15421 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
    15481 ctttttttat tt
//
LOCUS       DAYYCQ010000066        14966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000066 DAYYCQ010000000
VERSION     DAYYCQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.066"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..2382
                     /locus_tag="ACHAYF_002074"
     CDS             10..2382
                     /locus_tag="ACHAYF_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8579502.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2443..3903
                     /locus_tag="ACHAYF_002075"
     CDS             2443..3903
                     /locus_tag="ACHAYF_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8579503.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3914..6118
                     /gene="topB"
                     /locus_tag="ACHAYF_002076"
     CDS             3914..6118
                     /gene="topB"
                     /locus_tag="ACHAYF_002076"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8579504.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6134..6283
                     /locus_tag="ACHAYF_002077"
     CDS             6134..6283
                     /locus_tag="ACHAYF_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579505.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6951
                     /locus_tag="ACHAYF_002078"
     CDS             6358..6951
                     /locus_tag="ACHAYF_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8579506.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6964..7863
                     /locus_tag="ACHAYF_002079"
     CDS             6964..7863
                     /locus_tag="ACHAYF_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8579507.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7866..8363
                     /gene="ssb"
                     /locus_tag="ACHAYF_002080"
     CDS             7866..8363
                     /gene="ssb"
                     /locus_tag="ACHAYF_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8579508.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8729..8989
                     /locus_tag="ACHAYF_002081"
     CDS             8729..8989
                     /locus_tag="ACHAYF_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579509.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9434..9640
                     /locus_tag="ACHAYF_002082"
     CDS             9434..9640
                     /locus_tag="ACHAYF_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8579510.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9661..9891
                     /locus_tag="ACHAYF_002083"
     CDS             9661..9891
                     /locus_tag="ACHAYF_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579511.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9907..10344
                     /locus_tag="ACHAYF_002084"
     CDS             9907..10344
                     /locus_tag="ACHAYF_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579512.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10337..11044
                     /locus_tag="ACHAYF_002085"
     CDS             10337..11044
                     /locus_tag="ACHAYF_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579513.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11208..11408
                     /locus_tag="ACHAYF_002086"
     CDS             11208..11408
                     /locus_tag="ACHAYF_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579514.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11424..11603
                     /locus_tag="ACHAYF_002087"
     CDS             11424..11603
                     /locus_tag="ACHAYF_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579515.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11928..12191
                     /locus_tag="ACHAYF_002088"
     CDS             11928..12191
                     /locus_tag="ACHAYF_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8579516.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12185..12535
                     /locus_tag="ACHAYF_002089"
     CDS             12185..12535
                     /locus_tag="ACHAYF_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8579517.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12559..13173)
                     /locus_tag="ACHAYF_002090"
     CDS             complement(12559..13173)
                     /locus_tag="ACHAYF_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8579518.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13487..13909
                     /locus_tag="ACHAYF_002091"
     CDS             13487..13909
                     /locus_tag="ACHAYF_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579519.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13919..14122
                     /locus_tag="ACHAYF_002092"
     CDS             13919..14122
                     /locus_tag="ACHAYF_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579520.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14333..14917
                     /locus_tag="ACHAYF_002093"
     CDS             14333..14917
                     /locus_tag="ACHAYF_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8579521.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5515 a         2429 c         3037 g         3985 t
ORIGIN      
        1 ggaaagaaca tggcagaaga aaaagaacca ctagacaata gtcgtttggg aaaatccaaa
       61 agaaaactag tacgattgca aaatgaatta aacgaacaaa ttgaaaagat gtttgaacac
      121 caaaaaatga cgaacggtca accaatgaac gacaagagga atggccgttc atggtttaac
      181 cagcaagaac gaatcgaaaa taaagtccaa agtctgagag aagagatcaa gcaacaagaa
      241 gaatacgtgg aaaagcttga aagacaagaa gagctaaagg aaatgggcta taacaagtac
      301 ggcggactgg atatgacgat cgataatatt ccaagaatca aagaagaaat cgaacgcttt
      361 gaaaaaggtg aatcggcttt ttcagccgcc acgatccgaa aataccaacg gaagttggaa
      421 acattagaac agctgaaaga aaaatcagaa aaaggaaaag aaaatatcct ccctgaagta
      481 caagcgatca ttgacagcgg acgagtgacg caatggaaaa aaaatccaac catttatttt
      541 ttgaaaggct atcgaaaagt tgctttagaa ctgaatgaaa acgggggatt tgaggaatca
      601 aaaaaatacc aagccacgtc ggaagaggaa aaagcagctg ttcgtgaatt acttgaagag
      661 gctagaccaa ctgattctat gaaggagaaa gaaaacgatc atttctatcg agtagaattc
      721 aacgaaaatg atccagaaaa aggattgaaa agttatggtg gggagatcgt aacattcgaa
      781 ttgatcgatc aactcaaaac attggataac caatacgccg cagaagcagg ttattataaa
      841 ttctattttg aggaagtgaa gaacggagaa gtagtcgatc agatccgaat ggatctaggg
      901 gatggcgtca acagtaatca accaatctat ttagagttag caaaattgtg cgaacaagta
      961 cctgagaagg aacaaaaaca atcggtcaaa aatgaacctg tttcgaaaga acaaatgcag
     1021 tcgaaagaaa caaaaacaaa acaaaagggc tatcaaaaac gaacggcaga agagattaaa
     1081 caagaagtta aatccctttc gcaacaggca ctggatgcgg tgaaaaagca tacccattct
     1141 cctgaagacg tcaaagaact tttgaacttt atgtctcgtt ttccagaacg atcctttaga
     1201 aatcaattat tgattgagga acaatttcca ggtgcaacgg cttgtttagg tcgtgccgcg
     1261 ttgaaaaaag aaggaatcta tatcaaaaaa ggggaacaag ggaacaagat ctttgtcaga
     1321 aaaacggtca aagggtttta tgagaaagga cgtggctttg ttcgagaaac agaagctaca
     1381 ccagaagata aaaaacgaat cgaaagtggt caaatcgaag tgatcaaaaa accttactat
     1441 acgattgaaa aagtgtttga tatcacacaa acacagttaa aacctgaaga ctatccgaaa
     1501 atctttccga accgtgtctt tgattttcaa ttggataaaa aaggacagtc agaacttcaa
     1561 gctgggattc aagcggtagc caacaatatt ggaatacaaa ttcgagatat gacagaaagt
     1621 gaagtctatc aaagagaatt aggacaagca cgtggggcgt atgttcgtaa cgaatttact
     1681 ggcgaagaag aaatcgtcat gaatactcga aatactccta cgcagcattt agctacaagt
     1741 attcatgaat tagcccatgc aagaatgcac aaattttcag aattagatac cgcaactaaa
     1801 gaattgcagg cagaaatgac ttcttatatt gtgtgcaaac attttggaat ggacacgtcg
     1861 gagaaagcta ttccttatat tgcagcatgg acgaagaatg gtcagacgtt ggatgataag
     1921 gaagctgcgg aacgtgggaa aatcatgaat gatgtcagcc gtgtcgcaaa tgaattcatt
     1981 caaaccattt ctaatgaaat caatcaatac agagaaagag aaccagagat ccaaccagca
     2041 gaagtgaagc aggagcataa aaatacttta gaaacgccag agaataagaa tattgtgccc
     2101 gttggttcac tttggatcga caagaaagac gggaaagtaa tggaagaaga tacgggtcga
     2161 acgttacatt tgaaggatgc ggcgtttatt gaagatccgc agcataatca agtaatttta
     2221 aaaggaaaaa gttatggaaa agagatccaa acggcgttac cttcagaaga atttgcgcta
     2281 ggaaatccat tccgattttc acttgagaca gtgtcagaag tggcaaaacg aaaaaatgaa
     2341 atctattatg aacaacaatt gtcacaaaaa gaacgtgtat aaaacaagta aaaaagacta
     2401 tctgaaaaac ggatagtctt ttttcttttg ggaggtttta aaatgaccga gaaaaaaatg
     2461 agtcttattg atcgatgtaa acagatcgat atagtggatt ttgctcgaaa caatggaatg
     2521 gctgtagtca ataaaggacg agattatcgc ttagaagacc atgattcatt tgtttttgat
     2581 cgaagaaaac aacgttttta ttggaacagt caaaatatta gtggcgacat tattgaatta
     2641 gctaaacttt ttttcataga caaagagatc caagacccta agcaacaatt taaagcagcc
     2701 actgatttta ttctaaagaa tgaagacaaa acagaacgag tggaaaatct tcattttgaa
     2761 acagaaaagt acaaagatca ccccgttgat tatcaaccac tcacagaaaa aggaaggaat
     2821 tatctgaagg aagaacgaaa actaccagag tggctgattg attatgcaga aaaagaaggg
     2881 ttgattgccg aactaaagcc aaaatatgaa cggcaaaatt tcttagttcg agacgatcgt
     2941 ttagatcatg cggtcgcctt tttatggaaa gatccacaga caaaagagac agtaggggcg
     3001 agttatcaag gaacgttcat tgattatgaa cgttttggtg agagaggcac atataagcat
     3061 atagataaaa attcgacagc gaatcatggc tttaacctaa agatcggtga ccctaagcag
     3121 ataaaatttt ttgagagtag tattgattta cttagctatg ctgcactgaa tcgtgatcag
     3181 ttaaacgata cgtggttagt ctctatggaa gggttaaaac accatgtgat cagtcattat
     3241 tttggtgagg cagtatctga attgagaaag aaacaagcct ttcctcaatc catcgagatt
     3301 tgtgtggata atgaccgtgc aggacatatt ttctacgaaa aagaacaatt aatgggcgca
     3361 gtcgatccat ttacgaatca gaaagtacgt tgtgaacgag gaatcgctaa tgattggcaa
     3421 gtgccaaaag aatacaaaat catttatgaa gaagtcgcaa aagaagaaaa ggtcacacca
     3481 gaagctatta tggccataca caagacagaa aacaatctgc aattaacgaa ccaactggtt
     3541 tccgctcaca aagtaaatgc gttttttggc cagcagcttt ctgttaacga ttcaatagaa
     3601 gccattaatt tgaaagatat ttgtagagaa gtcgcaaaag aattaaaagt ttgtgaacgc
     3661 gtggatggta cgtacgattt tgatcgtttt tatcaagaaa aaggggatat taatgcgcag
     3721 attcttttct cttataaagc agaacaatac tacaaaggct ataaaaatca tgaacatgaa
     3781 tttgtacctg aagtaaaaaa ggactggaac gatcaattga agcatgagat ccagcaacaa
     3841 gaaatcagaa agcaaaaacg tgccatgttg tttcaacaag gcagacaaca agaaagggag
     3901 tgaaatcgtc atgatgaaaa ccgttatttt agcagaaaag ccgtcacaag cgaaagctta
     3961 tgccgatagc ttctctaaag ccactcgaaa agacgggtat tttgaaatcc aagatcgatt
     4021 gtttcctggt gaaactgtga tcacgtatgg ttttggtcat ttagtcgaat tagattcgcc
     4081 cgatatgtac gatgaaaatt ggaaacaatg gtcattagaa catttgccta ttttccctac
     4141 acattatcac tatcatgttc ctaaagataa gaaaaagcaa ttcaaggtgg tgaaacagca
     4201 acttcaatcg gccgacacca ttattattgc gacagatagc gatcgtgaag gggaactgat
     4261 cgcttggacg atcattcaac aagtaggcgc cgatcaagga aaaaccttta aaagactttg
     4321 gatcaactcc ttagaaaaag aagccattta tcagggattc cagcagttac gagacgcaaa
     4381 agagacctat ccaaaatttg aagaagcaca agcacgtcag atcgccgatt ggttaattgg
     4441 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagctt
     4501 ttccttaggc agagtccaaa ccccaacgct ttacatgatt tatcaattgc aggaaaagat
     4561 tagaaatttc aagaaagaac cttattttga aggaaaatcc gaagtaatcg ctcaaaatgg
     4621 cgcatttgat gcgaagcttg atccgaatga aacccaagcg acgcaagaag catttgaaga
     4681 ttatttaaaa gaaaaggggg ttcaattggg aaaacagcca ggcatgattc gccaagttga
     4741 aaccgagaaa aaaagcgcgg caagtccacg tcttttttct ctatccagcc ttcagtctaa
     4801 aatgaatcag ctcatgaaag ctagtgccaa agacactttg gaagccatgc aaggcttgta
     4861 tgaagggaaa tatctaagct atccgcgaac ggatacccct tacattacgg aaggggaata
     4921 tgcgtatctg ttggatcact tagacgaata caagcatttt ttgagggctg aagaaatttc
     4981 gactcctatt catacaccga atagtaggta cgtgaacaac aagaaagtcc aggaacatta
     5041 cgcaatcatt cctacaaaaa cagttatgac agaagcgacg tatgaaagtc tgacaccttt
     5101 acaacaagcg atttacgagc aagttttaaa aacgacagtg gcgatgtttg cggaaaaata
     5161 tacctatgaa gaaacaacga tcttgacgca agtccagcag cttcagttaa aggcaatcgg
     5221 aaaagtaccg atagatctag gttggaagaa gttatttggc aaagagagcg acggaaaaga
     5281 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtagatct
     5341 acaagtcctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     5401 tgcgatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     5461 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     5521 ggaatatatc aaggtagaga aaaataagct tgtggtgacg aataaagggg ttttgctttg
     5581 ccaagctgtg gaaaaagagc cgctgcttac gagtgccgaa atgaccgcca aatgggaaag
     5641 ctacttgtta aagattggag aacgaaaagg aacacaaacc acttttctag ccaatatcca
     5701 aaagtttgtc tctcacttat tggaagtcgt cccaggacaa atccaatcca ttgattttgg
     5761 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     5821 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     5881 gtctgaagcc tgtgatttta aattatggac aacgattgcc aaaaagaaac tcacagcgac
     5941 acagctgaag gagattattc aaaatagccg aacaagccaa ccagtgaaag ggttaaaagg
     6001 tcagaagggt tcgtttgaag caacgattgt tttaaaagaa gacttcacta caggctttga
     6061 attttctgaa aagaaaaaaa cgaacttcaa gaaaagaaca cgacgcacga caaaataatg
     6121 gagggttaat cttatgcgta atacaacaca caaacaacaa gaaatcgaaa atcaaaaagc
     6181 caaaattcgt tcagaacaag agaaattacg tgccagtaaa agaagacttc agcaattaaa
     6241 gaaccagcaa cggattcaaa aagaaatcga attagatcga tagacattaa ccttgttaca
     6301 aatacactat catgatatat ttaatgtagt gtatttgtaa caaaaggggg gaagtaaatg
     6361 caagagaaac tgaaagaatt gttggctatt cgcgatggaa atttatctat gaaggaagcc
     6421 agaaaattag gaatcgcagc aaccactgta caaagattgg tgaatcaagg aaaattgatc
     6481 aaagttgatc gtggttatta tgtagaggat ggtcatggaa tagatgattt attttggctt
     6541 cagcaacgtt tttccagggg aatcttctct catgaaacga ctttagatat ttaccagctt
     6601 tctacgaata tgccgaaatt cattcactta acttttcccc atggatataa cgttgatcgt
     6661 tcaattacaa aagagcagca gttacaattt catttcgtga agaaagaagt ttatgaacta
     6721 ggaaaagtag agggaacttc ctttcaagga aatcctatta ctatgtatga taaagagcgt
     6781 acactttgtg atatttggaa tccgagatat gaaattgaat atgaaatgaa attagaagca
     6841 ttaaaagagt acatggaaga cccattgaga gatccaagta aattaagaga ttacatgaca
     6901 aaactacatg tagaaaaaga aatgaaatac cacatgcaaa gcttatattg atggaggaaa
     6961 aagatgacac cgacacaatt tgcagcaaaa acaagaaatt tagcaaagag gacaggatta
     7021 aatgcacagt tagtacaacg gcattttatg atggatcgtt taattgaaca aatttctgaa
     7081 tctaagtata aaaatgattt cattttgaaa ggtggctttt tattaggatc aaaatatgga
     7141 atcgatagaa ggacgacaat tgatatcgac acgacattta gaaatagcaa attaactgaa
     7201 gaaaaattga agagtatttt taatgattta actaggaaac ccacgaaaga aggtattgtt
     7261 ttctctattc aaggaatgaa ggaaacaaga gaagccgatt tttatcctgg atttcaaatt
     7321 aaattggtgg ctaatttaga gaaagtacgt gtgccattta aaatggacgt gacaacagga
     7381 gattctattt atcctgaagt agatacacac tatcaccaat taatgtttga agataaaaag
     7441 gtggagattc cagcttatcc aacagcacaa attattgcag aaaagctaag tgctacgttc
     7501 agctttggca cagataattc gagagcaaaa gattactacg acttatttac cattccgaaa
     7561 ctagaaaata tcgatacgaa agagttatac aaaagtgttc gaaatacatt tacaaaacgt
     7621 ggggatacaa aaccgttgtc cttttattat gaaagagaaa tggataagat cataggaaat
     7681 gactatttgg aaaagcagtg ggaaaaatat cgctcagtta acacgtttgc gaattccatt
     7741 tcatttacag atacagtcaa cgaaattgat cacctaatga aaagtgtgat caaaattgaa
     7801 aatcaagaaa gaaaacggca gtttaaaaga tcacaacaaa tgaatcaaga aatggagaga
     7861 taaagatgat caataatgta actttagtag gacgcttaac caaagatcca gatttacgtt
     7921 ataccgcgag tggtactgca gtagcgactt ttaccctagc tgtcaaccga aatttcacga
     7981 atcaaaacgg caatcgagaa gctgatttta ttaactgtgt gatttggcgc aaaccagcag
     8041 aaacaatggt gacattagct aaaaaaggta gcttgattgg tgtagttggg cggattcaaa
     8101 cgcggactta tgacaaccag caaggacaac gtgtgtatgt gacagaagtt gtggccgata
     8161 atttccaact attagaaagc aaagcagcga cagaaagccg tgcgcacgct gatcaatcgt
     8221 ccaccagtcc aagcaccacc acttttgaac aacgggatac agcgacgcct aataacaatg
     8281 gattgaacgc tttacagaat ccatttggga atccatttgg tggacaatca atcgatatta
     8341 gcgatgatga tctaccgttc taagtagcat tttctattgg ttgcatttgc tacggtttat
     8401 gttataattt agtcaacaaa aagaattttc aatctataac caaaaaccac tagctggcgg
     8461 gctagtggtt ttcttttacg tttctattat aagaattaac gggtaggctt actgtctttc
     8521 ttggcaaatt tacaatcaag ccatttgcag atatagtgcg cggctacttc tgccatgatt
     8581 gcaaaaacaa aatcaaaaag aattttcaaa cctataacct cctccccttg ccagattagg
     8641 ccggggtagc agacagcaag actattatag cataattgaa atatccaaat gattggattt
     8701 ttgctataat tttagcgagg tgattgtgat gaacaaatat gttggtttgc ttgataaaat
     8761 ccgagtaata aaaacgcaac cgttattggt tcgctttact ttacaaacga ttcatgaatc
     8821 gattaattgt gtagttgcag atattgaaat tatcgataaa ttgctaatta tggatgatgg
     8881 aaaatacaat attgccgtca caggtcactt caataaacga aatcagctag ttattgctag
     8941 catgtatgtt agaaatcccg atcattttac gcgatcgatg ggcatataaa aaaagaagag
     9001 ttgcaatcct aatgtaggac tgcagctctt tttgttttac atagaaacaa gaaaaatgaa
     9061 aaggcctttt taggaaggcg cgagcgcctt ctcaattgaa aaacctctct cactccaacc
     9121 gccaccgccg ttcgagaaga aaggaagtga acgttgcacg ttcactaaga taaagggtct
     9181 tgcaaagcaa gacaaaatag aaaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
     9241 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
     9301 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgattcattc
     9361 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
     9421 ggcagaatca atcatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
     9481 cccaaactat catgcaccac atactgtcga atgcgattgt ggacaaagag cgaaatgtat
     9541 caaaagctat cgtttgtatc aatgtccaac atgcggaaaa aaatacacct tgtcttatgg
     9601 cgattatatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacata acataaatta
     9661 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
     9721 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
     9781 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
     9841 aacatggaaa cttatgaaaa cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
     9901 aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga taaaatggca
     9961 caaaaaatgg aggatatgac attcctatac catgagacac gtgttccaaa aaaacattat
    10021 aaggaaagat tgtcggtagc tgtggaagaa atgattgaat caggtgtcga aatgaatctc
    10081 attgcgacgt actatcgaac actggaagaa ctgaaaaaac aaaatgcaaa atggtttttc
    10141 caagccttac tatgcttgga agtaggtgtc aaaccaagca ctattaaacc ttctgaatat
    10201 caagctttag aactaacgta tgggaaattt gtcgaaacga aaaaagccaa gactgtttca
    10261 agtgaatggt tagattattt tgaaaatatc aataagtatg gcgcctatta caccatgaaa
    10321 aaggaggaca acgaaaatga ataaggtgat ttatcaacta acaacggaag atgtgtcaca
    10381 ctttttaaca aacaaagaac atttgaatga agtaatcaga cggattcaaa aagactttag
    10441 ttgtatggaa gaaatgaagt actttattca aagagtcgaa gaacaattgt atttggaaag
    10501 tgaaacgagt caaacgaagg tcacagtcag caataattat ttagacgtgt ataaacatcg
    10561 tcctgaaacg gtaaaaaaag gaacggagct tctacgacta gaaacagaaa aatacatcat
    10621 gaatgtttac ctagaaagga gtgaactggt cgaaaacttt gtcagctgca cgatttgcag
    10681 gaaacactat tactcagaat cagaatatta ttcaattttt gagagcatgg aattaatgga
    10741 agaagaaatg gaagagctaa acagcaaaaa tgcgacgata gacgattatt atcttgtatt
    10801 tactagattt ttgacgtgtg gaacggaatc tatcgtgtta atgggaatgg attatgaact
    10861 cgctgtaccc gaagcatttt tgagggaata tgtagaagaa gtagataacc caggcgacga
    10921 ctggtacacg actttaactg attttttaga tacctacact tacgacgata cagaagaaat
    10981 ccaaaatgaa cttgaccgta aacgaatgcc atatcaacga tttgaaagga aaaaaaggca
    11041 ataatcattt ataccgaaaa gtgagaatgt gtgaaataag aacaaaaacc ttgaaattaa
    11101 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    11161 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    11221 ttcctctgtt gttcgtactg tgtattggcg agatacttat gcgtcatggt gcagctgtac
    11281 atttaaactt cgtagtattc ttaggaatac tagtattttg tttgattttt tggcgctttg
    11341 tgttagccct tgtgagtcta ctattcaagt tgattgcagc catatttttc atagggttat
    11401 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    11461 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    11521 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    11581 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    11641 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat
    11701 aactatgaaa tctttaaagt atccagatca atttttttct tacctgattc taaagatttt
    11761 gttttcttaa acatattatg atttcctttc gactattttc atataaatgt taacatagtt
    11821 tatggattca tggaatgtta atctaattta atagaaaagg ataattaagt atatacgttt
    11881 tttcttatgt atgttatact atgtatatac agaaagaggt gtctactatg gaaatgttac
    11941 ctataaaaaa atggggaaat agtaatggcc ttagattgcc aaagtatatt atggaatatc
    12001 ttggaattca tacagaagac aaagtgaaga taatacaaga agaagtaaac ggtcagaagc
    12061 ggctaattat cgaagcggct aatatagaaa atgaattaac aattgaacag ttatttgaaa
    12121 attacactga tgaaagaaat catgtgacga ttcaaaattt aggtgaagca gtaggtaatg
    12181 aaaaatggtg agaaaaccta gacaaggcga tattctatta ttgaatacag cacctcgatc
    12241 tggacacgaa cagacaggta aaagacctta tattgtttta agtcatgata ttattgcgga
    12301 ttatagtaac gttgttacag ttgctccaat ttctagtaca actagaaact atccgctata
    12361 tgtaaatata aactcagaat atgaaatgaa gacaacagga aaagtattat tagatcaact
    12421 aacgacaatt gattatgaag ccagagagtg tatttttttg gaaaaagcgc atgatagttt
    12481 agttgaggag ttattgatga aagttagaac agtttttcaa aaagtaagca aataaaatag
    12541 aaacagcagc atgatcaact attcgtgatt gaccatgctg tcttttttta ttctatatac
    12601 ggtttgtctc gtaacattgt agtcttttgc aatcttagcg atcgcaacac ctttttttag
    12661 atcctcaacg atgtttttat aaacaagacg acgttgggga tcttttgcat tggcactgta
    12721 tagcttagga cgtcctttat aaacaccgtt cgcttttgca atcttaatgc cttgtgcctg
    12781 acgtcgttta gattcggtac gttcttgttc agcgatcatg gccaaaattt gaatgatcaa
    12841 atctttaatg aatttatcca ggagaggatt gccaattgct tcggccatga tcggtagact
    12901 tgtgatgatc agctgcacat ttttcttctt caagtaattg actgaagcga tgatctcgtc
    12961 gtaatttctt ccaagtcgat caatagcttc cacaataaaa atatcttgat ctcggacgaa
    13021 atccagtgct tcttgaaaaa caggtcgatg agtaatcgtt gcgccagatt gtttctcaat
    13081 gaaaattctc tctgcaccga attttttcat ttcttcaatc tgccgttgtt cattttgatc
    13141 gatggatgag acacgtatgt atgcaactct caaaattttt acctactttc tgcaactatt
    13201 taaaaaacac cctaatcata cacgagaaaa ccagattttt caatgtcgcg gtcgtgtcta
    13261 gttagggtat accttatata gacaaaaact aatgatggag gaaatagtat gtgtcacttt
    13321 tctttttaaa tggcaatatt ttttgtaaat aaatgtcaat caagaaaatt taaacgatta
    13381 tgacattaaa aatatatttt atgaattttc tagtatataa cttgatttat ctgttaagat
    13441 ttattaagaa aacgttttct tatttttgat ttcgaggtga tagacaatga aaaataagtt
    13501 tattttatct ttaggtattt taactattgg aattagtgta actggaacta caattcctac
    13561 atttgctgat agtacaaatg aaccaactac tgtagtagat aataaccaag aattaactcc
    13621 tttagatagg aacggaatag ccattggtaa cccaactaac gacttaactg ttactgaaaa
    13681 ttatttaata gtttctccat tacttgctcg agcagcttct aaaacgatac gagtgacagg
    13741 aacgatgatt tttgataaag ttggttggcc ttctagacct cctcaatcac ggaattggaa
    13801 agaaaaaaga aatggttatt ggtactatgg aaaattgtat ttacaaagtt ataaatcagc
    13861 tggaaacaat tggattgcga cttattcagg aaccttaact agaaagtagg gattttttat
    13921 gccagaattt gtgaacacac caaattttta tcaagatagc gatattctaa gtaaaattaa
    13981 tttaccgaaa gaagaatcag ctttacaagt tcagcgaact ttacaggaga cacaaaaaat
    14041 tcaaaaatca atttctaatc tagaagattc tgaagaaaaa agaaaaaaaa gagaccaaaa
    14101 aacatatcaa aaagaaaaat aatgatttta aaaaaagagt gtctagtcga cattcttttt
    14161 ttctaattga aagtgttcat aaaatcaccc atttctggac actttctttt tttgctttat
    14221 ttttcaaaaa aacaattcat ttcatgttca taaacccata acaatatcta tgttcagtta
    14281 aactatatgg ataaagttat gttacaataa aattaatgaa ggagtgattt ttttgaaaat
    14341 aggatacgcc cgggtatcta ctggtttaca aaatttggat ttacaagaag acagcctaaa
    14401 taaatttggt tgtgaaaaaa tttttactga tcatatgagt ggggcaaaaa gtaatcgacc
    14461 aggattagaa atggccattg attttgttcg ctctggcgat acacttgtcg tttggcgctt
    14521 agatcgttta ggtagaaata tggaagattt gatttcaatc gtcaatcgac tgaatgagcg
    14581 tggtgtgagt tttcatagta ttcaagaaaa tattacgatg gacaaatcaa gttctacagg
    14641 acagctcatg tttcatttgt ttgcggcatt tgcagaattt gagcgtaatt tgatcctgga
    14701 acgatccgca gcaggacgag cagctgctcg tgcaagagga cggtttggtg gtcgtcctga
    14761 aaaattgtct gaacaagatt tggaattact gaagacttta gtggatagcg gtacacctat
    14821 taagactatt gcggaacggt ggaacgtctc aagaaccacc atttatcgtt atttggcaaa
    14881 aatggaggag aaaaatggaa agacaaattc tcattagttt ttgtcattta atgctgaaaa
    14941 tttttgccat ttatctagaa aagtga
//
LOCUS       DAYYCQ010000067        14404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000067 DAYYCQ010000000
VERSION     DAYYCQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.067"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHAYF_002094"
     CDS             complement(227..1114)
                     /locus_tag="ACHAYF_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8579522.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHAYF_002095"
     CDS             complement(1111..1980)
                     /locus_tag="ACHAYF_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8579523.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHAYF_002096"
     CDS             complement(2117..3403)
                     /locus_tag="ACHAYF_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8579524.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHAYF_002097"
     CDS             3676..4254
                     /locus_tag="ACHAYF_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8579525.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHAYF_002098"
     CDS             4254..5183
                     /locus_tag="ACHAYF_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579526.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHAYF_002099"
     CDS             5180..6916
                     /locus_tag="ACHAYF_002099"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8579527.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHAYF_002100"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHAYF_002100"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8579528.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHAYF_002101"
     CDS             complement(7812..8321)
                     /locus_tag="ACHAYF_002101"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8579529.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHAYF_002102"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHAYF_002102"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8579530.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHAYF_002103"
     CDS             complement(9622..10713)
                     /locus_tag="ACHAYF_002103"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8579531.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHAYF_002104"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHAYF_002104"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8579532.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHAYF_002105"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHAYF_002105"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8579533.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHAYF_002106"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHAYF_002106"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8579534.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4099 a         3137 c         2606 g         4562 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agtt
//
LOCUS       DAYYCQ010000068        14378 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000068 DAYYCQ010000000
VERSION     DAYYCQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.068"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACHAYF_002107"
     CDS             complement(26..601)
                     /locus_tag="ACHAYF_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8579535.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACHAYF_002108"
     CDS             complement(606..1580)
                     /locus_tag="ACHAYF_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8579536.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACHAYF_002109"
     CDS             1863..2831
                     /locus_tag="ACHAYF_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8579537.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACHAYF_002110"
     CDS             2928..3221
                     /locus_tag="ACHAYF_002110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8579538.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACHAYF_002111"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACHAYF_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8579539.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACHAYF_002112"
     CDS             3678..4433
                     /locus_tag="ACHAYF_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8579540.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACHAYF_002113"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACHAYF_002113"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8579541.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACHAYF_002114"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACHAYF_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8579542.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACHAYF_002115"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACHAYF_002115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8579543.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACHAYF_002116"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACHAYF_002116"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8579544.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACHAYF_002117"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACHAYF_002117"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8579545.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACHAYF_002118"
     CDS             9170..9712
                     /locus_tag="ACHAYF_002118"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8579546.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACHAYF_002119"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACHAYF_002119"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8579547.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACHAYF_002120"
     CDS             11305..12207
                     /locus_tag="ACHAYF_002120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8579548.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACHAYF_002121"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACHAYF_002121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8579549.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACHAYF_002122"
     CDS             13650..14072
                     /locus_tag="ACHAYF_002122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8579550.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4759 a         2593 c         3102 g         3924 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaaga
//
LOCUS       DAYYCQ010000069        14220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000069 DAYYCQ010000000
VERSION     DAYYCQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.069"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHAYF_002123"
     CDS             complement(<1..1835)
                     /locus_tag="ACHAYF_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8579551.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHAYF_002124"
     CDS             complement(1832..2536)
                     /locus_tag="ACHAYF_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8579552.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHAYF_002125"
     CDS             complement(2548..4857)
                     /locus_tag="ACHAYF_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8579553.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHAYF_002126"
     CDS             complement(5052..5516)
                     /locus_tag="ACHAYF_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579554.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHAYF_002127"
     CDS             complement(5516..6142)
                     /locus_tag="ACHAYF_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8579555.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHAYF_002128"
     CDS             complement(6149..6514)
                     /locus_tag="ACHAYF_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8579556.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHAYF_002129"
     CDS             complement(6504..6842)
                     /locus_tag="ACHAYF_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579557.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHAYF_002130"
     CDS             complement(6832..7158)
                     /locus_tag="ACHAYF_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8579558.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHAYF_002131"
     CDS             complement(7139..7417)
                     /locus_tag="ACHAYF_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8579559.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHAYF_002132"
     CDS             complement(7419..8783)
                     /locus_tag="ACHAYF_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8579560.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHAYF_002133"
     CDS             complement(8796..9365)
                     /locus_tag="ACHAYF_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8579561.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHAYF_002134"
     CDS             complement(9337..10524)
                     /locus_tag="ACHAYF_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8579562.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHAYF_002135"
     CDS             complement(10588..12315)
                     /locus_tag="ACHAYF_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8579563.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHAYF_002136"
     CDS             complement(12312..12764)
                     /locus_tag="ACHAYF_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8579564.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHAYF_002137"
     CDS             complement(12876..13256)
                     /locus_tag="ACHAYF_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8579565.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHAYF_002138"
     CDS             complement(13253..13639)
                     /locus_tag="ACHAYF_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8579566.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHAYF_002139"
     CDS             complement(13641..13919)
                     /locus_tag="ACHAYF_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579567.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14220
                     /locus_tag="ACHAYF_002140"
     CDS             13982..>14220
                     /locus_tag="ACHAYF_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579568.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEG"
BASE COUNT         3938 a         3057 c         2239 g         4986 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
//
LOCUS       DAYYCQ010000070        14067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000070 DAYYCQ010000000
VERSION     DAYYCQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.070"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..225
                     /locus_tag="ACHAYF_002141"
     CDS             <1..225
                     /locus_tag="ACHAYF_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFM8579569.1"
                     /translation="VKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDK
                     LKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            337..1788
                     /locus_tag="ACHAYF_002142"
     CDS             337..1788
                     /locus_tag="ACHAYF_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8579570.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2137..2769
                     /locus_tag="ACHAYF_002143"
     CDS             2137..2769
                     /locus_tag="ACHAYF_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8579571.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3357..4796
                     /locus_tag="ACHAYF_002144"
     CDS             3357..4796
                     /locus_tag="ACHAYF_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8579572.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5091..5309
                     /locus_tag="ACHAYF_002145"
                     /pseudo
     CDS             5091..5309
                     /locus_tag="ACHAYF_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5558..5773
                     /locus_tag="ACHAYF_002146"
     CDS             5558..5773
                     /locus_tag="ACHAYF_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579573.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5990..6246)
                     /locus_tag="ACHAYF_002147"
                     /pseudo
     CDS             complement(5990..6246)
                     /locus_tag="ACHAYF_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6433..7344)
                     /locus_tag="ACHAYF_002148"
     CDS             complement(6433..7344)
                     /locus_tag="ACHAYF_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8579574.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7363..8367)
                     /locus_tag="ACHAYF_002149"
     CDS             complement(7363..8367)
                     /locus_tag="ACHAYF_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8579575.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8364..10487)
                     /locus_tag="ACHAYF_002150"
     CDS             complement(8364..10487)
                     /locus_tag="ACHAYF_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8579576.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10492..12939)
                     /locus_tag="ACHAYF_002151"
     CDS             complement(10492..12939)
                     /locus_tag="ACHAYF_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8579577.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12926..13315)
                     /locus_tag="ACHAYF_002152"
     CDS             complement(12926..13315)
                     /locus_tag="ACHAYF_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8579578.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13375..13878)
                     /locus_tag="ACHAYF_002153"
     CDS             complement(13375..13878)
                     /locus_tag="ACHAYF_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8579579.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
BASE COUNT         4366 a         2747 c         2553 g         4401 t
ORIGIN      
        1 gtaaaattaa gaattagctt tgaaaaaaat acaaagagaa tagtaggtgt acaactttgt
       61 agtcaagaag atttatctgg actattatca atgttttctc ttgctataga agaacaaatt
      121 acattagaca aattgaaaat gttagattat ttatttcatc ctagttttag ccaaccctat
      181 aattttattt taaaggcagt aacaggatta aacattgaaa cataatcgtt actaaatgaa
      241 atcaagaaat atactgtatt actcaattca ttgaaaatat ttatcataat ttttaataga
      301 aaacactatt aaagagaaaa aagagaggta aatcaaatga aaaaatcttt aaaacttgca
      361 tcagcagtta tattaatgtc ccaactcctt atagcaacac ctttccatgt gttggcaagc
      421 gaaaattcaa catacaaaat tgcaaaagaa agaaccgaga cgactgaaat gagtaattcc
      481 gatgtaacag cagagagtag tgaacttttg acaacttcat caagtgaaag tagtacgact
      541 tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc aaatgccgag
      601 tcttcaacaa cttcatcaag tgaaagtagt acgacttcaa atgccgagtc ttcaacaact
      661 tcgtcaagtg aaagtagtac gatttcaagt accgaatctt caacaacttc aagtagtaca
      721 agtgaaataa cttcttcgaa agtgacaaaa ccaaaccata tagaatcttc aaaaaaggaa
      781 gcgccaaaaa cggaaggaca aacaaaaact ttttcgacac cacagcaaat agaatcaaca
      841 gaatctttcc aatcacaacc aatactatct ggtaatgaag aaattcattt tgacaaaaat
      901 gaatctgtag aaaattttat tatgaaaatt ggtgagtctg cgagaaaaat tggtcagaaa
      961 tatgatttat atgcttctgt tatgattgca caagcgatct tagaatctgc cagtggtcaa
     1021 agccagttag ctcaggcacc gaattacaat ttatttggga taaaaggaac ttataatgga
     1081 aactttgtca ttatggtaac aaacgaagat ttgggtaatg gaacgcttta tacgacccaa
     1141 tcaaaattta gagtgtatga aaactatgag gaaagctttg aagattatgc caaattatta
     1201 actaagggaa ttagtggaaa taaagatttt tatgcaggag cattaaaagc taatagtaaa
     1261 acatatcgag aagcaactaa attccttaca ggacggtatg caacagatac acaatactat
     1321 ttgaagctaa atgaattaat taaaacctac gatttaacca actatgataa agaagttaaa
     1381 ggtccacaaa tgagtaagac tggataccaa gtgcctattc ataattattc aataaccagt
     1441 ttgtttggaa atcgtggaaa tgaatttcac cgaggacttg atatggcagc tacacagggt
     1501 gagccgattc atgcagttaa agctggtaag gttgttaaag cagagtttca ctactcttgg
     1561 ggaaattacg tggtgataca acacgaagat ggatcaaccg cgttatatgc tcaccagcaa
     1621 caatacctag taaaaactgg agataatgtt ttgcaaggac aaataattgg ctatgtagga
     1681 agtactggga atagtactgg cccacacctt catttggaaa tttgcaaaga tagtagtctt
     1741 tctcagagca tgttagttga tccgaaaacc ttattattcg gagactaaaa aaggtaatca
     1801 atctgacaaa gtaacaagaa aatggttgga ttcttagacc tttgatcagg atattgagac
     1861 tggaattgat atctcgatta cgatgggttt gacaaataaa acaagtccat tatcgaatat
     1921 cgagtgtatt attgccgtct ttatgctcat attttgagca ggtttcacta gatgaaaaca
     1981 atttatctat cttgaggatt tcgtgtacgt atcgttcagt cttgtattgt aaattagtca
     2041 taaaatcaga ctaagaaaaa ttggaaatat ttttttctga tttattaaaa gttaattgaa
     2101 attatatggt acttctttct tgggagggta ggtgcagtga aggaaagata tggtcacctt
     2161 actgttgttg aggagattac tcataatgga tataaaaaat atcgttgcct atgtgactgt
     2221 gggaatgaaa cgattgtata tcgatcaaac ttactatcag gtagaaccaa aagttgtgga
     2281 tgtggaaatt ataaaaatag gcataaatat aaaaatttga caaataaaac ttttggtaag
     2341 ttagttgcaa tcaagccaac taaactgcga agtgaaggaa ctgttgtttg ggaatgtcaa
     2401 tgtctgtgtg ggagaaaaaa gttagtatcg gcaaagcgac ttctaaaagg tgatgtaaaa
     2461 agctgtggat gtttgaaatc aaaaaagcat cacattgaaa ataaaaaatt cggattactt
     2521 actgcaaagt atcctttgga caataactgt tcatggaaaa caaaatgggt ttgtcattgt
     2581 gagtgtggca gtatgtgtga gataagttat agtaacttat tttttggaca tactcaaagt
     2641 tgtggatgtt taaaaaggca agaaacaagg acatgtgttg aagggacagt aatagagtgt
     2701 ctaacgtcaa aattaccgaa aaataataca agtggtgtta agggagttta tcgttctaaa
     2761 gagaaataga ttgcgtatat aactttaaaa aaaaagaggt actatttagg atcgtatgat
     2821 tctattaaga gtgcagctca agctagaaaa gatgctgaaa ataaattgtt tcaacctttg
     2881 attgacaaat atcgaggtaa tcattaatta ttgtggataa taattaacag tgatatataa
     2941 acatctataa tatattattc atactattga tatttagtat aatcctatgg aattgaatat
     3001 catagcaatg tatttgaaaa gcaaattgga aaacaaatag gtgcaacacc ataaatatga
     3061 aaaaattggg aaaaagtaga atggaaattt gcttacgtct cacgagatag ctcacactat
     3121 taatctgaag aacaatgaag tcgactttta gaaattgcta gaagaaagta aagcgaatta
     3181 tcactagtta ctatagagta agttcaaata aagcatctcc aacttacatg gttacatgag
     3241 gatatcaaat cgaaattact caagcttcct atggctagag tcgcgagaag ttgttgggca
     3301 gtagacatag gttatattgt tcttagctac acgatcatta gaaaggaaat cctattatgt
     3361 atagttatca agaagatgca aaagtccaaa aaaatcgctg gtggattttg attgctatat
     3421 ctatgtttac cttcatgtcc acattggatg gcagtattgt caatattgca ctacctacca
     3481 tctcagatga tatgggcata ccaatgaatc aagcggaatg ggtagtgtcc gtttatctga
     3541 tgattgtctg ttcttgtttg ttgctgtttg gtaaaattgg cgatagccgt gggaaaatca
     3601 aagtatttcg gattggtacg ctgatctttg tcttgggatc gctgttgtgt gggttcaatc
     3661 attcgctagg tattttattg ttcgcacggg tggtacaagc cattggtgct agtatgacta
     3721 tggctacaaa tgccggaatc atcacggaag tctttcccat acatgaacgt ggtcgggcat
     3781 tgggagcaat aggtgcgttt gtttcattag gctcgattgc tggaccaggt cttggcggct
     3841 tgattttgtc ccaattttct tgggcgtaca ttttttggat caatgtaccg gtgggaatcg
     3901 taacgatgtt gattgctgaa aaattcttac caaaagatat caccatgagc ggccatacaa
     3961 tcgatggagg gggctttgct gccattgcgg ctacgattat cagctttttt ggagcaatct
     4021 ttttggggca agaaagtggc tttttccatc cagtccccct tgtactgttc gtaatttcag
     4081 tgattgcctt tgtcagcttt ttaaaagttg aaaagaaagc agcctctcca ttgatttctt
     4141 tcggtatttt taagaacaag atttttacca tgagcttgat tacagccttg ttgatttttt
     4201 cttccaactt ctttgtcaat gtagtgattc ctttttatct gcaaaatgct cgcgggttga
     4261 gtcctagcta tgctgggctg ttgatgatgg tctttccttt gatgatggtc gtcggggcgc
     4321 ctttaagcgg ctacctgacc gaccgggttg gcccggagat tttggtatta gtaggcttgg
     4381 gcttattgtc ggtaacccag gtcatgtata tgtttttgtc agtggactct cctttgtggc
     4441 tatacattgt cgctactgcc atcatgggtg ccggcaatgc cttgttccag tcccccaata
     4501 atacgatggt tatgagctcg gtcactatcg aaaacttggg actggcaggc tcgatgaatt
     4561 cctttgcccg aaatttgggg atggtcagcg ggatttcgct ttctacgacg gtcttatatc
     4621 aagcgatgtc tgctaagatg ggccagcaag taactaacta tatcgcaaat cggccggatg
     4681 tctttttata tggaatgaag attacctttt taggcagctt tgttttgtgt tttgttgcct
     4741 ttttcctgac tatctggcgt atcaaaaaac aaggaggtat tcagaaaaat gtctgaaaaa
     4801 accactgttg aagaattttt ggaggcctgc gtatgggtga gtgggatttc catggatgat
     4861 ggaagatgaa gtgaacttct aaaagccgaa aaactcaagt attatcttca aaatactagt
     4921 gctcaatgag atgcttgatt tgatcacaag aagactgcga aagccgctgg caaccgcaag
     4981 ctaggtatcc caccgatgga ctacgtttta gaggctactg gttatgaata cggagccaac
     5041 gctctcatct gcataagaat acatcaccca gattatgtaa agattttcga tacttctgtc
     5101 aaaaaatcat taagatagat aaatgatttc cgtctagtca aattggttca gaatgtagac
     5161 acacagatgg taagaagtca ctcacaattc gagaatggac ttgtcctatt tgccgtatac
     5221 atcataatcg aaacgtcaac gatagtatca atattttgac caagggctat gaatacaagc
     5281 attgccttag aaaacatcaa gaaccttagg aacaacgggg atagcttggt taataagaga
     5341 taccgctgtt taggcttgca agtatactct tttatcaaga attccgtggc tttagtcatg
     5401 agaggcttaa tactatgaaa attttcttta gcatttttga tttaggttcc ttttctaatt
     5461 ataataagat gaccactaca gtaacctact gaaatatata aacagccact gtagtaccat
     5521 tttcaaagta gtctatgaga ggagaatctt cattcttatg aacgctggaa ttgcggatat
     5581 gaatttaata aaaaaaaccc ttaatgattt tacttccaat tcaatatcaa aaggtactgg
     5641 tatcaattta agcacaataa aaaagctgaa aagtggtgaa agatcagtgg aaaagctgaa
     5701 tcttttagat gctataaaaa taacggaatt tgctatgaaa aatggaaaag ctgagattga
     5761 aatatggaga taattatcgt ctagtgaatt acttgccact tagctgaatt taacgatcct
     5821 tggtgcttga agggggagat tcttgggaat agagcatact tgcaagccta tgtctttact
     5881 aacaacgata tctcttatta accaagctat ccccgtagtt ctaaggcttt tacttttatc
     5941 taagcaataa tcgtattcaa aggaaatttt tgacccttag aaagtctcat tttatcagac
     6001 gtgagcttag gcattgctga attttgttaa ttcggtttta ggttgtgctt tgagcataat
     6061 atgaaagtat tctttatcac gatttcattc atctaaggta atgtgatgtg attctgaaat
     6121 tctttcaaag gcagtttttg cataagctga cattacatca tcaatgacat gtttacaatg
     6181 tttcactact aaaataaggg ggtaaccaag aagaaaaact gaatgattat ttgtatctaa
     6241 ttccatggta tatcaaccat ttcctatata gactgattat gacatggatt aaaataaaaa
     6301 ggcactatgt cttaattgtc ggttttcgag ttaccgaatg gcattcatca cccacctata
     6361 gaggatgggc gacttctgcc tgaaataagt tacactactt ataacttcta ataaaattag
     6421 attgagtcat tattatttaa catcaacgat tttccagttt ccattttttt gtaatactaa
     6481 tttgtactga aagtagctag tggtttttga tacattatca aggtatttta tctttgtact
     6541 cactagaaaa ttttttccgt tttgtagata aactggattc tctaactccg aaaactttaa
     6601 gtcaatattg attggtcgca tcgcaccatt ttcaacataa tactccaatt ctttttcaga
     6661 agctgatgga taaagtttaa aaaatgtttc taaaaaatcg gtgatttctt ccgttgtttt
     6721 ggcatcaatt tctgaatcgt tttctacttg ttttgcttca tattttgcct tgtctggctt
     6781 tccaatcatt gttggcaaac tagttactaa atgattcctg ttttcatctt caaagattgt
     6841 cactcgatat gcagaagaaa ttgattgcgt cttttttccc tctgtaatat gttgctccac
     6901 taaaaatgaa acgacaaatt cttccgagtg tttttcaaca tctagaattt ttacagattg
     6961 aacttcggaa ctcgtgggaa tatctgctcg aaccatatct tggattaaag cgagaccgtt
     7021 ttctgtaagg tactgttcga gattactcat tctcttttca attgcttctt tgttattttt
     7081 ccatgaaaaa tattctttcg caaaatcctc tgtaaaactt tcaacgccag aagtattcac
     7141 taccttctct tttataacca ctttttcatg agttgtatgt tgatcaatgg cggtgaaatt
     7201 tttataaacg ccaaaagcaa gactacaaga caataaaagc caaagggcaa gcaccatttt
     7261 tcgatgctta cccacggata caactcgttc ttttttcttt tttgggctct tttccttttg
     7321 attccgttca atttttatct tcattctttt tcctcttttc tcttatttgt atcggccagc
     7381 tccgataaga tgctgccgcc aatagttttc cgttagattc gtccacccca ctggatctcc
     7441 tgcatgaaac atgcgattct cccctgcgta aatgcccaca tgggtcacat aagtaccggc
     7501 atcataggtt gaatgaaaga aaactaaatc acctggtttg gcatctttta gatcaatatg
     7561 agtcatggca tcatattgat cctgagccac ccgaggaagt ttcaggtcaa ctttggcata
     7621 acaccattgg gtcaagccac tacaatcaaa ccccgtttct ggacttgagc caccaaacac
     7681 ataggccgtt ccttcatact taaatgcctc atcaaaaatt tcttggacta ttttgtcatc
     7741 aaactgagtt gtagttaggt attgcttcac taataaacag taaaacatat tgccataatt
     7801 ataccgccac ccaccatttt ccttaatcgc gattgggttg gggtaatcga ctttttgacc
     7861 acctgatttt tcctttgaaa aagattcagc taaagcaaaa ctatgcttct tcccgttttt
     7921 cccgacatga tctagatacc cgccaccata gttataggcc tgaataatac tatcttgatc
     7981 acaccctagt tctttggaac gcttaaccaa agacgcaaaa tagagacagc cttgtttgat
     8041 ggatgcctcg gtacttaatg aattaggtgg taatccggct gactcagaac tttgcataac
     8101 gtcttccatt gtgccaccag attccacttg aataatcgcc aaaagaattg gaatatactc
     8161 tgttatatcc gattctttgg catacttttc caccattgca cggtgtttca atacttcttc
     8221 tgagacacta gttccgccag gagaagtgcc agaactacca tttccctcac tgtcttcacc
     8281 aaacaacaaa cccacacaca tcaaaatacc cataaaagag atggtcagaa aagttaatga
     8341 aataacaccc actttcttca agttcatttt ttctcacctg accttgattt tggacgatgg
     8401 ttcattcttt tttgtacttt ttgattggtc tgctttggca gagtggacct ctgtttcact
     8461 tgttggaaag aatttttctt acttgctgat tggcttttcc cttttgaaat gatttcttga
     8521 ctagttagtt ttcgtttgat ttcgggattc agatttgctc tcacaatccg ctttggtgct
     8581 tcttttttca gcatcatatc tttctgaggt tgagctgctt tatctattgg aatattcgcc
     8641 cttctttttc cctgacgatt tgttgcttca ctcagtatct tgcgtttttc gtccattttc
     8701 gcatttcttc gttgtcgttg tttttctcgc aaatccactc gatccgcttt ttcttggaac
     8761 agtccgcgct tgaattcttc tggtgctttt ttcgactttt cgattccttt atggagattg
     8821 tatttcaaat tcgttggcgt attccttacc tgatccttta cttgtttagt cttatctttc
     8881 accaaagctt gggtatccaa aactttgccg atttttctcc cagccaaatt cattcgggat
     8941 tgtttcttat tatttgaaac tggattctcc ttagttaagt cagatagtat ttcgtgatcg
     9001 ttcggtaatc gtgaattctt tcgtagagaa gaaccggatg gcttaaattt ggattttgat
     9061 tgttcctttg ccaacttatt tccgactgct gcaccaccac caaaagccaa agctcgtccc
     9121 acatttcgat tgagttgccg caattttcga ttcattaaca tttgcggttt tcgcatgacg
     9181 cgtcttccca ttgactggct atcattactc tggaggttga acatactcat aatgtcgccc
     9241 aatttgaaat agataccggc aaaggtcaca atttgcagaa aagcaaccat gaaaaatgga
     9301 taggttgccg aaatattgaa aaacatcgtg gagatgctaa atgcagtcgt aatcaccaaa
     9361 gtaacacccg ctcgcatcat aatcgtattg aataaacgaa taatcgcctt ctttcctaaa
     9421 ctttcataag tcggcaacat ggacaataag aaactaattg gcagaaacat cgcaaagata
     9481 atgaagagaa tttgcgagaa caacataatc cctgttagaa gaaaaacaaa gaaggaaatg
     9541 ccaatattga acaataccaa aaagaccacg gtccctaaac ggttcatggt cttggtaata
     9601 ctgagattcg cattgtcatt gtcttcaatc tctgccttga ccgcttcttc ccgatctttg
     9661 cctttgtttt catctggact cacagataga atcttgttga cccggtcttc accaatttct
     9721 tctatgttgg aatcatcaaa ttgcagtagt aaccaaggtt gttggacttg aatcgaaaac
     9781 aaactatccc gaatcaaatc cacactatct tttccttggc tttctgaatt gggtacaacg
     9841 atctcggttc caagggtaag ggccgcttca ctaacatctg agctaaagtc attgattttt
     9901 gtgatatagg taggcgcata agcaatgaac gaacccgata gtagaaaaat catcacaaaa
     9961 ttaatcacgg ctcgaactgc tttcgtggtt tctcgtttta ataatccggt atacgccaca
    10021 tacaccccca gagctaaaat catcaataat agaaacccaa aataaaatcc ggaggtctgg
    10081 agtccatttt cagtaatacc agccaatgtt tggatattct ttccaatact ttcagccgta
    10141 tccgaaatga aatctaattt gtaagcctct tgaaccacat aacccgtggc gttcgacaaa
    10201 tacaaactca ccgtccaaat gaaattagta atggcataaa gaccatacat cacgcttttc
    10261 cctaagccat caccccaatt ccaaggcaac caatcccaag agctatccac aaagaaatct
    10321 agttgatagt tgttcagtga atatttggaa tatagatttc ctgcttcaac tgtatcatca
    10381 actagccctg tcgcctcggc taccgtacca actaaactca atagaactat cagagtgagt
    10441 atgctaataa aaacgattcc cacaatccga aaaagtttct ttttcatccc ctcactccat
    10501 tctctcttga acaggtggac gggtatcaaa ggcatggaag agttcttcaa agactggatg
    10561 tacctgaacc acgcctaccc gaccatacaa atcctgcatc aagcattggc cattttccaa
    10621 atctcgtagg cgtttttgat tgctttcatc ttctcgatcc aagccaaaga attccaaggt
    10681 tttcttgatt tcatgaatat cggtactgcg aaacgcaaac ttcaacccga tattattctt
    10741 caagcgttca tccagtaaat catcactatt ttgcgtgaca aagtaaaccc cggcattcat
    10801 agcccgccca gcacgaacga gcttattcga taatgttttc ccttgcgcta cttgaagaaa
    10861 actccacgcc tcatctaagt ctacaatttt gaagatttct cgattactat ggataaagtc
    10921 caaggcaaaa gtggaaatga cgattagcat ggaaacacta agcaattcca tggtggtata
    10981 ttcttcaaaa gtggtttctg catctggtaa aaccaaatcc gctacttgaa tgatatttaa
    11041 ctgcctatct aaactaatcg actgggtaat cgaaccatct gagaaaagca aatgggcaaa
    11101 atcataatcg gtaaaggact cgatgtgatc cgcgatattt tccgcaactg gtgagccttc
    11161 ttttcgaagc tcattaatta cttgtaataa tccccgttga ttgctttggg taaccgtccg
    11221 aatggcctta cgcaacactg gaaacttctc cccatctcga ctagaaattc ctgttaaaaa
    11281 ggtcagaata tcaattgcta gactttcaga atccttcttt ttctccataa tcacataagg
    11341 atcaagtaat ccttgatttt ttggcttact agttaagttg agaatattga tttcatccgc
    11401 aatttctggc aaggtttctt tccagccacc ccgttcccct tttggatcga caatcacagc
    11461 ttgtccacca aaaaggacgg cgtaataaac taagagattg ttactaaagg actttccgcc
    11521 accgagtgaa ccgaggaatg ccgctgctag cgcattggta actgaatcct tgaccccttg
    11581 tgaggccaat gccggtttca ggtaaacatt gcgccccgta tctacgttat aaccaaagta
    11641 aatgccttcc aactccccta acatctgagt ggcgccaaaa ccaagtccgg cgagaaaatc
    11701 agacgtaaca tactggatat aatcattcat gtatcgctta ctagaaggta agaattcttc
    11761 atgtagcccc atcatatcgc caaaaggccg gactagtttt acattggtat catcataaaa
    11821 atctagcact tcatcacacc ggcgctttag ttcatctact gattcggcac ttactcgaac
    11881 cacatagctc agtttgtaca tggattcttt ggactgatcc aaggtagttt ccaattcatc
    11941 tacggaatcc aatgcatcta agacattgga atttgtttca ttgtcagatt gataggcatg
    12001 gttgtccaag tcttttaatt cctttttctt attgcgaacg gtagcaagtg cctttttgtt
    12061 cgtcacaatc tccacattca tactagtgtc aattgggaat gtaaattgtt gttgctggta
    12121 atagaaaagt tctgaggagg gaaactccat ttcccccaca attgtattaa ttgttaagta
    12181 cgctacatat gattcatgat tctcatgttc catgcataag taccggggct tttcttcaat
    12241 caaacaacga ctgggacgta ataagtcgta acgtttcacc aaagtttccg acttcagctt
    12301 tttctttggt agctgaaatt catattcttc aaatggtgta cccttttcgc catagatatg
    12361 ttcaatcaaa tacatcagat cactcggtgt gactcgtctg actttaaatc ttcgagccaa
    12421 tttactttcc attagttttt caagcttggt atatcggcga atttcttcat tggataagga
    12481 aacaaaatcg cccatcaaat gatgattcac tccatagaca aattcttgaa agccggagaa
    12541 gaaagatttc ttgagattct ttagattgac ctcttcatct gtcgcaatca gtttgaagcc
    12601 aataaaaaag cgataatcaa tctgactatc gccaatcatg gatactaatg cctctgtctg
    12661 taaatcaatt cgttgtttcg ctacttctgt taatcgcccg gtaatttcct tttttgatcg
    12721 ttcttgcgtg gcacgaatac tactttctgt ggcaatttgt aaggcatgaa tcttgccttc
    12781 acgattttgc gcaatcaatt gacgaaagtt atcgtggact tgatatttct gttcaggtga
    12841 caaaaaggag taattgtaag gtattaactc atagtaggca aaacattctc cctcttgatt
    12901 aaagacaaga ttattttcaa tatacttaat cgggtacata gttcacactc cttaccagag
    12961 taatgggttc gtcccatcgt ttcttctgtt ctttgacttt ctttcctgcg aaagtgacct
    13021 taaatcgtaa gtgatagcta ataaaagatc gtaaaaatcc caatggtttc ttaccgtcaa
    13081 acgttttttg cgacatgaac caagtaactg ccaccggtat acctaagtat tttaaaaagg
    13141 ccccctctat catagatagt ggaggcacat gggcaaatac gatgacaaac agcaaggata
    13201 aaacaaacca agccatctgg ttaaaagtca caggaaaagg cagttgaaag tcattaattg
    13261 cataaatgac cttttcaact gcccaaatac tggtgtagct ttttattttc ttcatcaaat
    13321 cattccttta aaaagaagga tagtttagaa atcaactacc cttcaataat tttgttaata
    13381 tggatactca aaaacacctc gactcgttac caaataattg cctccaatct ccaagtctct
    13441 accacacgca tcaaaatcaa tatacgtctt caagtgttct ggtacttctc caaaatcacc
    13501 agcttctaca atcaagaaat atgctacatc actcatatca tcgcaatctg gataatagat
    13561 aatatcatcc acatgctcta ccatctcctc aaaagaattg aagaaagcat gttgaatctc
    13621 agaagcaact tcaccaactg gcgttccttc aagttcctcc gctaagccac acaaccgatt
    13681 aatttcctca attggcgtat actcatcaat ctcaaaggga agttcataat catgaattgc
    13741 atattcctcg tattcgtcat ttaacccaat tcgttctttc acttcatcaa aatcgacagg
    13801 tggggtaaac cacgccccca ccaattcacc ttcgttatat ttcccgaggt tcgcaatata
    13861 aacacgcatc tgttccatcg tgcttccccc ttacgcaccg acaattcggt taaacaaact
    13921 gagtaagaca tccttcaccc cagaagcatt aaagaccaat ccgaccgcaa tcaaagcaat
    13981 caccaaaaag ccaatcagtt tggaaaactc ccgcttaaat cctaaaaaca aaccaatgac
    14041 cacaattgcc agtaagacta aggattg
//
LOCUS       DAYYCQ010000071        14024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000071 DAYYCQ010000000
VERSION     DAYYCQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.071"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            299..2464
                     /gene="acm"
                     /locus_tag="ACHAYF_002154"
     CDS             299..2464
                     /gene="acm"
                     /locus_tag="ACHAYF_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8579580.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2736..3221)
                     /locus_tag="ACHAYF_002155"
     CDS             complement(2736..3221)
                     /locus_tag="ACHAYF_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8579581.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3311..3656
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3701..4342
                     /locus_tag="ACHAYF_002156"
     CDS             3701..4342
                     /locus_tag="ACHAYF_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8579582.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4361..4996
                     /locus_tag="ACHAYF_002157"
     CDS             4361..4996
                     /locus_tag="ACHAYF_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8579583.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5014..5829
                     /locus_tag="ACHAYF_002158"
     CDS             5014..5829
                     /locus_tag="ACHAYF_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8579584.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6148..7584
                     /locus_tag="ACHAYF_002159"
     CDS             6148..7584
                     /locus_tag="ACHAYF_002159"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8579585.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7773..10205
                     /locus_tag="ACHAYF_002160"
     CDS             7773..10205
                     /locus_tag="ACHAYF_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8579586.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10435..11502
                     /gene="rlmN"
                     /locus_tag="ACHAYF_002161"
     CDS             10435..11502
                     /gene="rlmN"
                     /locus_tag="ACHAYF_002161"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8579587.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11492..11698
                     /locus_tag="ACHAYF_002162"
     CDS             11492..11698
                     /locus_tag="ACHAYF_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579588.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11695..12798)
                     /locus_tag="ACHAYF_002163"
     CDS             complement(11695..12798)
                     /locus_tag="ACHAYF_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8579589.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13350..13898
                     /gene="aac(6')"
                     /locus_tag="ACHAYF_002164"
     CDS             13350..13898
                     /gene="aac(6')"
                     /locus_tag="ACHAYF_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8579590.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4821 a         2308 c         2957 g         3938 t
ORIGIN      
        1 tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg aaatatagat
       61 tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat aatttacatt
      121 aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat atttgtaaaa
      181 taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt gaagaaagga
      241 tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga tagaaaaaat
      301 gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata taacatcact
      361 gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg tcacttcgtt
      421 gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat tctcgtttga
      481 cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc caagtgaagg
      541 aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa ataaaaatgt
      601 tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa atattaaaaa
      661 cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg taaccgatac
      721 cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag ctactgtaaa
      781 tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa caggagacat
      841 gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata acggcactta
      901 tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat tagatccaag
      961 tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag gagaagaggg
     1021 aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag taactgataa
     1081 ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa tggtgagtta
     1141 taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg aagcttggtt
     1201 taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag ttaaaaatat
     1261 ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt tcaaatatat
     1321 caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag cagatgaaca
     1381 gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag aagctagtat
     1441 caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca attggattga
     1501 ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg atacagaggg
     1561 agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa ctaaaatttg
     1621 gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag caagtacaaa
     1681 caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa taacttcagt
     1741 gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt ttaaagtgca
     1801 agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa ttgaaaacgg
     1861 gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa aaacatggga
     1921 agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc tattagccga
     1981 cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct atcgtttcac
     2041 aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg aagacaacgt
     2101 gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata caccggaaaa
     2161 aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc cagagaagac
     2221 agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg ctcatacagg
     2281 agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta aacagataac
     2341 aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt ttgctgtaat
     2401 cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat ataaaaaagt
     2461 ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat aaaattccga
     2521 tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga atcaataaat
     2581 atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca tactagttgt
     2641 tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata aagaaaaaga
     2701 cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata aactggcaga
     2761 tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa cgtagtggta
     2821 ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc gatcacgact
     2881 tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc tgctggaacg
     2941 cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc ttttaattgt
     3001 tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag atagatttgt
     3061 tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag ggaaaaaggg
     3121 acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat acccagtttt
     3181 tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca ccctcctttt
     3241 tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta aattacacgt
     3301 aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt agagagtctc
     3361 tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg attttctgaa
     3421 gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta gatgaccaga
     3481 taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc
     3541 tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt
     3601 tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc ctcttggcat
     3661 ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt tgattgaagt
     3721 tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat ttaccttgat
     3781 tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt ttaagccgct
     3841 acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt tgttgcaact
     3901 gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc
     3961 actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc gtggagggat
     4021 acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa gtccctttca
     4081 aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt ctgtagggaa
     4141 tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt taggtgacgt
     4201 aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt tgctaggcgt
     4261 ggcaatcatc tatcttacat tgactggtat tctaacaatc gtaatgaaac aagttgaagg
     4321 aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat tgaagaaagt
     4381 gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca ttccagatgg
     4441 gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt tacgtatctt
     4501 ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac cgttcaaccc
     4561 attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact tccagctatt
     4621 tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat tgaaacagac
     4681 aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga ttagtgattt
     4741 attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag cgattgccag
     4801 agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg ctttagatcc
     4861 tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg gggtaaccca
     4921 aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt
     4981 ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt tctattcgct
     5041 ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc agcagataat
     5101 tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac atttgttcct
     5161 atgggattta gagataaaga cgggaagctc acaggatttg atattgattt agccaaagca
     5221 gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc aatgaaggaa
     5281 tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa aacgccggca
     5341 agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt gctgatcaca
     5401 cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt aggtgcgcaa
     5461 aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa agagatagtc
     5521 aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga tctgaaaagc
     5581 agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt gaaacaaaga
     5641 aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga ttttgcagtt
     5701 ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct agatcaacta
     5761 gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga tgttacgccg
     5821 agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa
     5881 acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc acaacctctt
     5941 ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc aaaaatgtag
     6001 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc
     6061 ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat
     6121 tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc atttccaaaa
     6181 gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg gcaagaagat
     6241 gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac atttaaagga
     6301 acgaacggtg atttggcagt agaccactat catcgttata aagaagatat tgctttaatg
     6361 aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat ttttcctaat
     6421 ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat cgacgaattg
     6481 attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc acaagcattg
     6541 caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac tcattatgca
     6601 gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt gaatgaacaa
     6661 aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt gacagacccg
     6721 aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt catcaataaa
     6781 tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac gccaaattat
     6841 ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga cctgatggcc
     6901 aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat gaggttctta
     6961 gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct agaatctgct
     7021 aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt caatccatta
     7081 aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaagttcatc agaagaaact
     7141 ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac aaattgggac
     7201 tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag ccgttaccgc
     7261 atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac agaagataaa
     7321 caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc catcaaagaa
     7381 gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac agatttgctt
     7441 agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca agacgaaaca
     7501 caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca aaaactgatt
     7561 gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac ggctggatat
     7621 ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa aataagctga
     7681 aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac ttttttttgt
     7741 ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg cctactaaaa
     7801 aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa atgctgacga
     7861 ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa ttaggttttt
     7921 taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac caaatcctct
     7981 gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag ttttcaattg
     8041 gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta ttattgttac
     8101 atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg acgacatggg
     8161 atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt ggtgggatga
     8221 ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga agttatttag
     8281 cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa ggctatcagc
     8341 tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa ggtgatcctg
     8401 ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc gcagaagcga
     8461 aagaagcaag gcgcttagct gcaaaagaag ctgcagaata tgagaagaaa aaagcggtga
     8521 gccagcaaag ggaaaatact gctgatgaat ggcaggaacc agaaaataca gagccggagc
     8581 agctaagttt tgttccaatc gacagttttc aagaaaatat ccatcctgcg aatctagaga
     8641 aaccagtacc agatacacca aaacagacca atacagcaga aggtttttca gatgagttgc
     8701 cagaggatga cggtacctca ttagaatttg agattgaagc agaacaggaa aatcaagact
     8761 atgaattacc gacagttgat ttgcttgatt ctataccaac tgtggatcaa agtgatgagt
     8821 ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg
     8881 atgccaaggt cgtcaaagcc agcttaggac catccgtcac aaaattcgaa gtccagccgg
     8941 ctgtaggggt aaaagtaagt aaaattgtga acttaacaga tgatatcgcg cttgctttag
     9001 cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa atcactgatt ggtattgaag
     9061 taccaaatgg aaagatcagt atggtttcct ttagagaaat catcgaagca caaccaaacc
     9121 acccagataa attactagaa gtacctttag gacgtgatgt ttctggacgg gtacaaacgg
     9181 ctgatctttc gaagatgcct cacttactcg tggctggttc gactggtagt gggaagtcag
     9241 tagcaattaa tgggattatc acaagtatat taatgcgtgc taagccccat gaagtaaaac
     9301 tgatgatgat cgatccgaaa atggttgaat tgaatatgta caacggcata cctcatctgt
     9361 tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag
     9421 aaatggaaga acgatatgaa aaattcgcgg caaccggagt aagaaatatt tctggttaca
     9481 atgaatttgt tcaacagaaa aacttggaaa acggaacgaa acatccaacc ttaccattca
     9541 tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt agccagcaat gaagttgaag
     9601 atgcgattat tcgtttagcg caaatggctc gggcagcggg gattcatatg atcctagcta
     9661 ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa agcaaatgta ccttccagaa
     9721 tggcatttgc ggtatcaagc ggtacagatt ctcggacgat cattgattct aatggggcag
     9781 aaaagctctt agggcgagga gatatgctat tcttgccaat gggtgagaat aaaccgatcc
     9841 gtgtgcaagg agcattcatt tctgaccacg aagtagaacg ggtcgttcaa tttgttacag
     9901 atcagcaaga agctcattat gaagagaaaa tgatgccgac agatgaagtt gaaacgacag
     9961 gtgctccaga acagccacaa gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa
    10021 tgcaaacggc aagtatttcc cttctccaaa gaagatttcg gatcggttac aaccgagcag
    10081 cacgtttagt agatgaactg gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc
    10141 ctcggaaagt attcattgag caggaagaaa catcagaaac gttgccggac gataatgaca
    10201 tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc aataaaaaga caaggcatga
    10261 tgataccaaa agaaaaaatg gtttttttcc atcttttcta atacggtatg atgtcttgtc
    10321 tttttagttt ttgtgagatt aaagttctat tcgatttatt tgtgaagtat tgcattaagg
    10381 aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa agagggaaaa tactttggaa
    10441 aaacagtcca tttatggctt gacgaatgaa gaattaatca attggtttat agaaaacggc
    10501 gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat
    10561 ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa aattatcgga gcattttatc
    10621 atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg
    10681 tttgaactcc ctgataaaaa catgattgag actgtactga tgagacaaga atatggcttg
    10741 tcagtttgtg tgacgacaca agtaggatgt aatatcggct gtacgttttg tgcaagcggg
    10801 ctgctcaaaa agaaccgtga tctgactgct ggagaaattg ttgcacagat catgatggtc
    10861 caacattatt ttgatgagcg caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg
    10921 atcggtgagc catttgataa ttacgacaat gtaatggact ttttgcatat catcaatgat
    10981 gccaaaggat tggcaattgg tgctcggcac attacagttt ccactagtgg attagcacac
    11041 aaaatcaagg aattcgctaa taacggcttg caagtcaatc tggctatttc cttgcacgcg
    11101 ccgaataatg aggtgcgaac atcgatcatg cggatcaacc gaagtttccc aattgagaaa
    11161 ttgatggaag ctgtggatga gtatttagag aaaacaaacc gtcgaatcac gtttgaatat
    11221 atcatgttga atcaagtaaa tgatagacca gagcatgcgc aacaattagc agatctatta
    11281 aaagataaga aaaaattagc atatgtcaac ttgattccat ataatccagt cagtgaacat
    11341 gatcaatatt ctcgcagtcc aaaagctgat gtattgagat tttatgatgt attgaagaaa
    11401 aacggcgtaa actgtgtgat acgtaaagaa catggtacag atattgatgc agcttgcgga
    11461 caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt
    11521 ttcaattacc ataaaaggag cagttttctt aattaaggaa ctgttcaact taaataagag
    11581 aaaagattct atagcgaaag ctataaagct ttttttgaca agaaaaaccc tcatcaagca
    11641 aagaaatgtc ctcaatttga tgacagcctg cttgatgggg gttttagcaa ttatttaact
    11701 gattttctta tatccttgct ggctttcttg tgtagcatga taagtttcta cgtcaagaat
    11761 ggcctgttta aatttttctc gggtgatttc ataaggcaca tgtttaatat catttgttgc
    11821 catggcttta tctaagattg cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa
    11881 cgatactgga agttggttct ttttcataaa cacatagatt ttatcaaaca aatcgaactg
    11941 ctgatccatc atcaataagg tcagtacacc ataactgaca gatgctccgt gaaggtatcc
    12001 ctccattttt tctagaacag tcgtaccgta atagaatgag tgtgccatag cagaattata
    12061 gttattgtta actaaaacag aaaccaatcc ggtagtaaca atgatatccg aaacgacttg
    12121 ttcaattgca tatgagggac gatcgtcggc actatctttc aatgcttgcg taccataagt
    12181 caagagacga tcaatgcaca tttttccgat ttggattcct aatctatccg tgtagcctag
    12241 atcatcgcca cgagcagaaa aagtagattc gacttctttg gataaagcat cgccaatacc
    12301 tgcccataag tatttatcag gtgcttcggc aataattgct gtatcaatga aggtgtgtac
    12361 aggtggtttt gaaggaaaga agagtccgtc cattacgcct tcttcgttat agatgacaca
    12421 taccgcagtg acgggcgaac agttcgaacc aatagtggga aaggtgaaga ctggtttagc
    12481 tagtttttca ccaatgactt ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag
    12541 gacgtctgct ttttggacac tttcatgctg gatcaactgt tcaatatttc cataagttga
    12601 attacccccg taccagagac tggcgacaac ctctatttcc tgtgtcaaag ctacttttat
    12661 tttgttttct gcctttgaaa gtgcagtctt ccctccaatc agcactactt ttgtcccata
    12721 ttctttgacg attccatttg ctttttcata cgcatctgtt ccaatggtat aatttggtaa
    12781 gaaaacactt tgactcatat tcatttcctt ctttcttcgt tgaaacgggt taaaatttta
    12841 aagagtgatc gatctgactg cggctgtaga tggagtagag gtgaacctaa gacaggataa
    12901 gtgatgaata aggtggcatg aacaccttga aatcatcaat aagtatccta aaaacgaaca
    12961 agtacaaaaa cattgaaaac gacaataatg aatttttgtt cctcttagta tttctaccga
    13021 ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa ttaagattat tatatgaaaa
    13081 caggttttat ttgtcaattc ataattggtt ttttttaaag caaaagataa tctatatcat
    13141 gatgaacaaa cagagttttt tcctccaatt gatggctttc ctgatctttc ctattttgaa
    13201 taaagtgttt ctgtataatt ttttatttta taagtaagat aaataatgaa agaatagaga
    13261 tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt
    13321 agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc
    13381 cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag
    13441 acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg
    13501 tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt
    13561 gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat
    13621 tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta
    13681 cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg
    13741 ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag
    13801 gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga
    13861 tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag
    13921 taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa
    13981 ttccaactta tttccgaaga agctactttt ggaacaccgt gtac
//
LOCUS       DAYYCQ010000072        13998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000072 DAYYCQ010000000
VERSION     DAYYCQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.072"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..310)
                     /locus_tag="ACHAYF_002165"
                     /pseudo
     CDS             complement(119..310)
                     /locus_tag="ACHAYF_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(320..1135)
                     /locus_tag="ACHAYF_002166"
     CDS             complement(320..1135)
                     /locus_tag="ACHAYF_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8579591.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1397..1840)
                     /locus_tag="ACHAYF_002167"
     CDS             complement(1397..1840)
                     /locus_tag="ACHAYF_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8579592.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1974..2312)
                     /locus_tag="ACHAYF_002168"
     CDS             complement(1974..2312)
                     /locus_tag="ACHAYF_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8579593.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2300..2677)
                     /locus_tag="ACHAYF_002169"
     CDS             complement(2300..2677)
                     /locus_tag="ACHAYF_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8579594.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2901..4094
                     /locus_tag="ACHAYF_002170"
     CDS             2901..4094
                     /locus_tag="ACHAYF_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8579595.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4260..4682)
                     /locus_tag="ACHAYF_002171"
     CDS             complement(4260..4682)
                     /locus_tag="ACHAYF_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579596.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            5077..5271
                     /locus_tag="ACHAYF_002172"
     CDS             5077..5271
                     /locus_tag="ACHAYF_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579597.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5268..5714
                     /locus_tag="ACHAYF_002173"
     CDS             5268..5714
                     /locus_tag="ACHAYF_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579598.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5924..7096)
                     /locus_tag="ACHAYF_002174"
     CDS             complement(5924..7096)
                     /locus_tag="ACHAYF_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8579599.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7302..8117)
                     /locus_tag="ACHAYF_002175"
     CDS             complement(7302..8117)
                     /locus_tag="ACHAYF_002175"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8579600.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8267..8713)
                     /locus_tag="ACHAYF_002176"
     CDS             complement(8267..8713)
                     /locus_tag="ACHAYF_002176"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8579601.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8710..9225)
                     /locus_tag="ACHAYF_002177"
     CDS             complement(8710..9225)
                     /locus_tag="ACHAYF_002177"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8579602.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(9298..10212)
                     /locus_tag="ACHAYF_002178"
                     /pseudo
     CDS             complement(9298..10212)
                     /locus_tag="ACHAYF_002178"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10209..12536)
                     /locus_tag="ACHAYF_002179"
     CDS             complement(10209..12536)
                     /locus_tag="ACHAYF_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8579603.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(12724..13911)
                     /locus_tag="ACHAYF_002180"
     CDS             complement(12724..13911)
                     /locus_tag="ACHAYF_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8579604.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         3965 a         2763 c         2543 g         4727 t
ORIGIN      
        1 aaatcattca tagggaagtc ctcttttctg tgaattggtt gtcgttaact ttattctaca
       61 gaagggactt ccttttttgt atggattttt tcatttacac aaaatatttt atactctcga
      121 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
      181 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      241 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      301 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      361 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      421 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      481 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      541 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      601 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      661 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      721 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      781 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      841 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      901 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      961 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
     1021 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
     1081 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1141 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1201 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1261 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1321 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1381 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1441 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1501 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1561 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1621 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1681 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1741 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1801 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1861 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1921 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1981 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     2041 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     2101 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2161 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2221 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2281 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2341 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2401 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2461 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2521 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2581 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2641 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2701 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2761 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2821 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2881 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2941 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     3001 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     3061 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3121 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3181 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3241 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3301 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3361 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3421 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3481 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3541 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3601 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3661 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3721 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3781 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3841 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3901 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3961 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     4021 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     4081 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4141 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4201 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4261 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4321 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4381 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4441 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4501 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4561 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4621 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4681 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4741 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4801 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4861 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4921 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4981 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     5041 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     5101 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5161 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5221 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5281 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5341 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5401 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5461 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5521 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5581 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5641 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5701 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5761 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5821 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5881 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5941 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     6001 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     6061 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6121 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6181 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6241 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6301 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6361 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6421 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6481 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6541 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6601 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6661 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6721 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6781 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6841 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6901 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6961 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     7021 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     7081 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7141 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7201 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7261 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7321 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7381 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7441 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7501 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7561 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7621 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7681 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7741 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7801 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7861 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7921 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7981 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     8041 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     8101 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8161 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8221 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8281 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8341 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8401 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8461 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8521 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8581 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8641 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8701 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8761 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8821 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8881 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8941 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     9001 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     9061 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9121 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9181 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9241 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9301 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9361 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9421 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9481 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9541 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9601 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9661 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9721 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9781 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9841 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9901 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9961 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
    10021 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
    10081 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10141 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10201 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10261 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10321 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10381 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10441 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10501 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10561 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10621 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10681 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10741 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10801 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10861 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10921 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10981 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    11041 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    11101 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11161 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11221 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11281 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11341 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11401 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11461 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11521 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11581 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11641 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11701 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11761 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11821 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11881 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11941 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    12001 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    12061 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12121 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12181 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12241 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12301 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12361 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12421 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12481 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12541 cctctcaata aaaagtcaag tccacaatct tgtgtaaaag ttcaacctca tcaaaagcac
    12601 aatgttttgc gttgtgaacc taactgccat gcctttcttt ctgaggagag aggtcaggag
    12661 cagtagcgca gcgactcttg atgtctctcc tcagaaagaa gagactcgca caggttcatg
    12721 gtgctaaatg ttattcgaat taaattctaa atattttcgt ggagaagcca gccactcagt
    12781 gtctttttcc atcaaaagtg ttccaattat ccgagtgatg gaattgatat ttggaaaaat
    12841 ccgaatcact cgttctctcc ggcgaatctc ttcgttcaac cgttccaaca cattggttgt
    12901 acgaatcctt cggcggatat tttcaggaaa agacaaaatt tgaatagcat cttcaaagcc
    12961 gttttctaaa atgtcgcaag cagcacttaa ttttgaatca caaccatatt tttccaagaa
    13021 gtgctctttt ctttcacgtg tcaattcaag ttcagaggct ttaaagatac tcttcaactc
    13081 gtcttttaca tcagaagaaa ctttctttgg cagcttatca aaaatatttc tcaaaaagtg
    13141 ggcttggcat ctttgccagc tcgcacctaa aaagttcttg cgaacagcct taactaaccc
    13201 tttgtgtgca tcagaaatga ccagttttac accgaacaaa cctcgttgaa cgaacgattg
    13261 aaaaacggta tcccaattat cttccgattc gccgtcttga acatcaaaac caagaatcgt
    13321 acgtcgtcca tcagaatcaa tgccgatacc aatatataca ccttttgaga caatacgatg
    13381 attttccctt actttcatat acagggcatc acacatgaaa aatggatatt ccgagccttc
    13441 taggctccgg tttctccact caaaaacaaa tggatccaac tgtttcatca gatttgaaac
    13501 gaatgattta gaaacagtcg caccatccgt tagcagttca actgttttag ttactttgcg
    13561 agtggagaca ccagaaatga ccatttcaat catggcagcg atcaaagctt tttcgcttcg
    13621 ctgatatctt tcaaagatag taggactaaa cttaccgtct ctggttcgtg gaacatgaag
    13681 ttctaacgta ccaattcgag ttgtaaacga acgatcgtag taaccatttc tttgtgattg
    13741 ccgttcttct gaacgttcat aggcatttgc ctgaatatat tgatttcgtt gttcttccat
    13801 cagctgatta aacatgatag tcataagtgt ttttgcggta tcattcgcac cgctttcgtt
    13861 tataattgct tgaatttctt cgctttcaaa agtaaaatgt acttgagtca ttgatatcag
    13921 tcctattcga gtatgttttc tgcaaaaaac attgtactcc taactgagat caatggcttt
    13981 tttgatgctt cactttta
//
LOCUS       DAYYCQ010000073        13917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000073 DAYYCQ010000000
VERSION     DAYYCQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.073"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHAYF_002181"
     CDS             131..505
                     /locus_tag="ACHAYF_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8579605.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHAYF_002182"
     CDS             complement(619..1248)
                     /locus_tag="ACHAYF_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8579606.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHAYF_002183"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHAYF_002183"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACHAYF_002184"
     tRNA            1668..1741
                     /locus_tag="ACHAYF_002184"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACHAYF_002185"
     CDS             complement(1879..2043)
                     /locus_tag="ACHAYF_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579607.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACHAYF_002186"
     CDS             2338..3666
                     /locus_tag="ACHAYF_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8579608.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACHAYF_002187"
     CDS             3868..4701
                     /locus_tag="ACHAYF_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8579609.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACHAYF_002188"
     CDS             4717..5454
                     /locus_tag="ACHAYF_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8579610.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACHAYF_002189"
     CDS             complement(5609..6577)
                     /locus_tag="ACHAYF_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8579611.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACHAYF_002190"
     CDS             6809..7642
                     /locus_tag="ACHAYF_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8579612.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACHAYF_002191"
     CDS             7676..8515
                     /locus_tag="ACHAYF_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8579613.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACHAYF_002192"
     CDS             8541..9101
                     /locus_tag="ACHAYF_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8579614.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACHAYF_002193"
     CDS             9300..11021
                     /locus_tag="ACHAYF_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8579615.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACHAYF_002194"
     CDS             11186..12328
                     /locus_tag="ACHAYF_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8579616.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACHAYF_002195"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACHAYF_002195"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8579617.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAYYCQ010000074        13867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000074 DAYYCQ010000000
VERSION     DAYYCQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.074"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..306
                     /locus_tag="ACHAYF_002196"
     CDS             112..306
                     /locus_tag="ACHAYF_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579618.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="ACHAYF_002197"
     CDS             575..874
                     /locus_tag="ACHAYF_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579619.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="ACHAYF_002198"
     CDS             complement(971..1405)
                     /locus_tag="ACHAYF_002198"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8579620.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="ACHAYF_002199"
     CDS             complement(1637..2347)
                     /locus_tag="ACHAYF_002199"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8579621.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="ACHAYF_002200"
     CDS             complement(2337..3197)
                     /locus_tag="ACHAYF_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8579622.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="ACHAYF_002201"
     CDS             complement(3202..3840)
                     /locus_tag="ACHAYF_002201"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8579623.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="ACHAYF_002202"
     CDS             complement(3855..4154)
                     /locus_tag="ACHAYF_002202"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8579624.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="ACHAYF_002203"
     CDS             complement(4197..5681)
                     /locus_tag="ACHAYF_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8579625.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="ACHAYF_002204"
     CDS             complement(5701..6162)
                     /locus_tag="ACHAYF_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8579626.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACHAYF_002205"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACHAYF_002205"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8579627.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="ACHAYF_002206"
     CDS             7465..8235
                     /locus_tag="ACHAYF_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8579628.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="ACHAYF_002207"
     CDS             complement(8508..9335)
                     /locus_tag="ACHAYF_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579629.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="ACHAYF_002208"
     CDS             9903..11138
                     /locus_tag="ACHAYF_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8579630.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="ACHAYF_002209"
     CDS             complement(12137..12397)
                     /locus_tag="ACHAYF_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8579631.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="ACHAYF_002210"
     CDS             complement(12424..13725)
                     /locus_tag="ACHAYF_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8579632.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4467 a         2834 c         2176 g         4390 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgcatga caacgcaaag aatgttcata actaattata
    13801 taattaagaa attttatttc aatatattct cttaaataat caaatttatt tttaagaatc
    13861 ctgaaaa
//
LOCUS       DAYYCQ010000075        13625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000075 DAYYCQ010000000
VERSION     DAYYCQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.075"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..267
                     /gene="yaaA"
                     /locus_tag="ACHAYF_002211"
     CDS             25..267
                     /gene="yaaA"
                     /locus_tag="ACHAYF_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8579633.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            254..1378
                     /gene="recF"
                     /locus_tag="ACHAYF_002212"
     CDS             254..1378
                     /gene="recF"
                     /locus_tag="ACHAYF_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8579634.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1375..3321
                     /gene="gyrB"
                     /locus_tag="ACHAYF_002213"
     CDS             1375..3321
                     /gene="gyrB"
                     /locus_tag="ACHAYF_002213"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8579635.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3341..5812
                     /gene="gyrA"
                     /locus_tag="ACHAYF_002214"
     CDS             3341..5812
                     /gene="gyrA"
                     /locus_tag="ACHAYF_002214"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8579636.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6048..6347
                     /gene="rpsF"
                     /locus_tag="ACHAYF_002215"
     CDS             6048..6347
                     /gene="rpsF"
                     /locus_tag="ACHAYF_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8579637.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6397..6909
                     /gene="ssb"
                     /locus_tag="ACHAYF_002216"
     CDS             6397..6909
                     /gene="ssb"
                     /locus_tag="ACHAYF_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8579638.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6935..7171
                     /gene="rpsR"
                     /locus_tag="ACHAYF_002217"
     CDS             6935..7171
                     /gene="rpsR"
                     /locus_tag="ACHAYF_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8579639.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7314..9287
                     /locus_tag="ACHAYF_002218"
     CDS             7314..9287
                     /locus_tag="ACHAYF_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8579640.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9293..9745
                     /gene="rplI"
                     /locus_tag="ACHAYF_002219"
     CDS             9293..9745
                     /gene="rplI"
                     /locus_tag="ACHAYF_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8579641.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10019..11386
                     /gene="dnaB"
                     /locus_tag="ACHAYF_002220"
     CDS             10019..11386
                     /gene="dnaB"
                     /locus_tag="ACHAYF_002220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8579642.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12166..13458
                     /locus_tag="ACHAYF_002221"
     CDS             12166..13458
                     /locus_tag="ACHAYF_002221"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8579643.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4541 a         2467 c         3003 g         3614 t
ORIGIN      
        1 aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac ggaattcatg
       61 acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca agcaaaatgg
      121 tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa
      181 ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa
      241 ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa attacgaaga
      301 gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg cgcaaggaaa
      361 aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc ggacaagtag
      421 tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag agatcacaaa
      481 aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa aaaccaagat
      541 caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg tcattttatt
      601 tgcaccagaa gatctttctt tagtaaaagg cagcccgcag atcaggcgga agtttttaga
      661 tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat accaagcaat
      721 tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa ctgatttgct
      781 ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat tgaaagcaag
      841 agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc aaatcaccca
      901 tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag aaacacatac
      961 gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa agaaagacct
     1021 ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt ttattaatca
     1081 gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac taagtgtcaa
     1141 attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc ttttgttaga
     1201 tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa caatcgaagg
     1261 aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata aaatgactgt
     1321 tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca
     1381 gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat tcaagtactt
     1441 gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac tagttctgag
     1501 ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt attagcaggt
     1561 tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt tattgacgac
     1621 ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt tgaaacggtc
     1681 tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga
     1741 cttcacggag taggatcttc tgttgtaaac gcgctttcta cacagcttga tgtcaaagta
     1801 tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta
     1861 aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc agaccctgaa
     1921 atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat ccgtgaactt
     1981 gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga agaaccagtc
     2041 atcaatgaat accattatga cggcgggatc aaaagttatg tcgaatactt gaatgcaaac
     2101 aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga tatcgcagtt
     2161 gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt tgccaacaat
     2221 atccatacgt atgaaggtgg aacacatgaa tcaggattca agactgcttt aactcgtgtg
     2281 ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa cttgaccggt
     2341 gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga ccctcaattc
     2401 gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac tgatcggtta
     2461 tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg gcaaattgtt
     2521 gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg
     2581 agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga ctgctctagc
     2641 aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg cggatcagct
     2701 aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat
     2761 gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc tttatttact
     2821 gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta ccacaaatta
     2881 gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct gctgactctt
     2941 ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc tcagccacct
     3001 ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa
     3061 gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag cgttcagcgt
     3121 tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat ggacccaagc
     3181 aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca gatatttgaa
     3241 atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa tgcacactat
     3301 gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag aaatcagaga
     3361 aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg attatgcgat
     3421 gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac ccgttcatcg
     3481 ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc ataaaaaatc
     3541 tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagaca ttgcgatcta
     3601 tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag tggatggtca
     3661 tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata ccgaagcacg
     3721 gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg ttgatttcca
     3781 aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct tcccgaatct
     3841 tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc cacctcataa
     3901 cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg tgacgaccaa
     3961 tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg ttatggggaa
     4021 atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac gtgcaaaagt
     4081 tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat tgccttatat
     4141 ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca agcgtatcga
     4201 agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg tcattgacat
     4261 ccgtcgtgac gccagcgcat ccgttatctt gaataactta tataaattga catccttgca
     4321 aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt
     4381 gaaacaaatc cttgaaaact atatcgaaca tcaacgcgaa gtcatcacac gacggacggt
     4441 ctttgaaaaa gaaaaagcag aagcaagagc acacatctta gaaggtttgc gtattgcttt
     4501 ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg acgaggcaaa
     4561 agcaacaatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta ttttagatat
     4621 gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat accaagaact
     4681 cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg ttacagaaat
     4741 catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac gcacagaatt
     4801 actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag aagaaatcgt
     4861 tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat tcagagctca
     4921 acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact tcgtcaaaaa
     4981 tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg gaaaagtgta
     5041 tcgggcaaaa ggatacgaaa ttccagaata cgggcgtaca gccaaaggaa tcccaatcat
     5101 caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg tgacgggaga
     5161 agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc
     5221 tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga
     5281 cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta ttggaacaca
     5341 taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac gtacagcttc
     5401 cggggtcaga gggatacgcc tgcgtgagga agattatgtc gttggggcag ccttaatgaa
     5461 ggaaaatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga caaaagtatc
     5521 ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa
     5581 aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca tgctgattac
     5641 agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg gacgttcaac
     5701 attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga tggcagcagt
     5761 cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat aaccatcaag
     5821 actaagtgtg taagctagca agaataaccg attaaaaaat ctggttattc ctgctggctt
     5881 ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta ttgcttttga
     5941 ccaaaataga gtgtataatt agagcttgtg agtagtctaa ttaattagat aactctcctt
     6001 gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg gaaaacacga
     6061 aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa actgctttaa
     6121 tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa tcaaaagatt
     6181 gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc tatcacatcg
     6241 ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa
     6301 tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg ttgtttcatg
     6361 tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt tgtattagtt
     6421 ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc agtcgcaaca
     6481 tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga ggctgatttt
     6541 atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc tcgtaaagga
     6601 acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca gcaaggacaa
     6661 cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag tcgttctgca
     6721 tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc tggcggattt
     6781 ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc tgatttcgac
     6841 cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga cgatgattta
     6901 ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa gaggcggacg
     6961 taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg attataaaga
     7021 tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac
     7081 tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat
     7141 gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat gactgatcct
     7201 tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg tttagtcgct
     7261 cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa attatggaaa
     7321 ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata ttaatcgaag
     7381 tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga
     7441 atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta aataaacgtg
     7501 aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc tcagaagtca
     7561 tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg ctcaatccat
     7621 tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta gatacttttt
     7681 ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca tttcagtttg
     7741 atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac cagttgttaa
     7801 aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat aactatgcag
     7861 atgccattga taaattagat gacaaagaga tttcttattt gaactctttc gtcactacac
     7921 tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac gcagaacggt
     7981 ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa tttgatttgc
     8041 tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt agtatgggga
     8101 ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat aatttagatg
     8161 ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac
     8221 ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag
     8281 cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt atcatggggc
     8341 acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt ctggcacgct
     8401 tcaaccagaa aaaagcttac atcgttatca atgagaacga gatcattcca gatgtagaac
     8461 ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc tctccagaac
     8521 gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac cataaacctt
     8581 ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc attgatcacc
     8641 atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatattgaa tcttcagctt
     8701 cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa acaaatcgct
     8761 tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca aatagtttca
     8821 atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct tgtggagcag
     8881 acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg gagatcagca
     8941 atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca gggacagacg
     9001 aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt tcgatgatca
     9061 atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg atcagtgcca
     9121 gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc ggaggacact
     9181 tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa cagaagctat
     9241 ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt
     9301 aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag aagttccagc
     9361 aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa cgaaaagcag
     9421 tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga
     9481 agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg aattgaaggc
     9541 gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg
     9601 gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag aaaatccgat
     9661 ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga cggctacgat
     9721 caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct ggtaagtata
     9781 accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag caatgaaagc
     9841 aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca gcaaaatagc
     9901 ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc ggtacgaatg
     9961 atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag cgatacgtat
    10021 gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac aagcggtttt
    10081 aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca tagagccaaa
    10141 agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt taaatgatcg
    10201 taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca atttgttaga
    10261 agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga cagcagcaaa
    10321 tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat tgatccagac
    10381 agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa caattttaga
    10441 tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc
    10501 tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc aaaacaatga
    10561 agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag caggtttcca
    10621 gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt
    10681 gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat ttagtttgga
    10741 aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga ttgaggccag
    10801 ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg ttgcgatggg
    10861 gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa tctctgaaat
    10921 ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga tattgatcga
    10981 ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag tatcagatat
    11041 ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg ctctttcaca
    11101 gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg atattcgtga
    11161 atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag atgattatta
    11221 tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca acgtcatcga
    11281 agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac tgtttatcaa
    11341 agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga
    11401 gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc aggaacgact
    11461 agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg tcacagtccc
    11521 tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag caaacgtcaa
    11581 aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatctta
    11641 cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc
    11701 atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg
    11761 ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc
    11821 aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga tgcaggacag
    11881 ctttttcaca acctctgatt cacggtgttc caaaagtagc ttcttcgaaa aataagtcga
    11941 aatttcacaa aaatttgaga agcaattttc gtacattcct tcttatttct tgaagctgac
    12001 cacttctgtt acaacctctt ttttaataac cattctaaaa agataaagtt cgttttttaa
    12061 gcgtttttat aaaataaaaa taaaaatcat tcgattttat ttgaaatatg tatctattat
    12121 tgatacaatg gttcaggatt aaataatata gaacgaggtg ttcacatgtc atctgtagta
    12181 gtagtaggta cgcaatgggg cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa
    12241 aatgctgaag tgattgcacg gtaccaaggt ggcgacaatg cagggcacac aattaagttt
    12301 aacggtgtga cttataaact gcatttgatt ccttctggga ttttttataa agacaagatc
    12361 agtgtgatcg gaaacggcgt tgtagtcaat cctaaatcat tggttaaaga attagcttat
    12421 ttacatgaaa aaggtgtaga aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc
    12481 ttgccttatc atatcaaatt ggaccaatta caagaagatg ctaagggcga aaataagatt
    12541 ggtacaacga ttaaaggaat cggacctgcc tatatggata aagcagctcg tgtaggtatc
    12601 cgtattgcag acttattaga taaagaaatt tttgaagaac gtcttcgttt gaatctagat
    12661 gacaaaaacc gtcaatttgt aaaaatgttt gattcagaac cacttgattt tgacgatatt
    12721 tttgaagaat attatgaata cggacaacaa atcaaacaat acgtcacaga tacttccgtt
    12781 atcttgaatg atgctttgga tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta
    12841 atgctggata tcgaccaggg aacttatcct tttgttactt cttctaaccc agtagccggt
    12901 ggggtaacaa tcggaagcgg tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc
    12961 aaagcctata cgtcacgtgt cggtgatgga ccatttccaa ctgaattatt tgatgaaaca
    13021 ggagaacaaa tccgcgaagt aggacgagaa tacggtacga caactggtcg tccgcgtcgt
    13081 gtaggctggt ttgattctgt agttatgcgc cattctaaac gagtttcagg tattaccaac
    13141 ctatctttaa actcgatcga tgtattaagt ggattggaaa cagtaaaaat ttgtacggct
    13201 tatgaattag atggcgaact aatctaccac tatccagcaa gcttgaaaga gttgaaccgc
    13261 tgcaaaccaa tctatgaaga attgccaggc tggtcagaag acattacagg atgcaaaaca
    13321 cttgcagaac tgccagaaaa cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt
    13381 gtacgtatct caacattttc tgtcggacct gatagaacac aaacaaacat cttagaaagc
    13441 gtctgggcgc aaatctaagg ataaaaacga cgactgaatc aggacgttct taccagagcg
    13501 agtcgaagtg tttatctata cgataataat aacttgaaga ggtagtgaaa gagacgttta
    13561 gccttattgc cgataggctg tctcttgaac actgtttatt cagaataagg gactgtgaca
    13621 aaaac
//
LOCUS       DAYYCQ010000076        13428 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000076 DAYYCQ010000000
VERSION     DAYYCQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.076"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..743)
                     /locus_tag="ACHAYF_002222"
                     /pseudo
     CDS             complement(45..743)
                     /locus_tag="ACHAYF_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            822..998
                     /locus_tag="ACHAYF_002223"
                     /pseudo
     CDS             822..998
                     /locus_tag="ACHAYF_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1174..1743)
                     /locus_tag="ACHAYF_002224"
     CDS             complement(1174..1743)
                     /locus_tag="ACHAYF_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8579644.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1817..2140)
                     /locus_tag="ACHAYF_002225"
     CDS             complement(1817..2140)
                     /locus_tag="ACHAYF_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579645.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2294..3106)
                     /locus_tag="ACHAYF_002226"
     CDS             complement(2294..3106)
                     /locus_tag="ACHAYF_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579646.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3433..4308
                     /locus_tag="ACHAYF_002227"
     CDS             3433..4308
                     /locus_tag="ACHAYF_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8579647.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4322..4645
                     /locus_tag="ACHAYF_002228"
     CDS             4322..4645
                     /locus_tag="ACHAYF_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579648.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4642..5394
                     /locus_tag="ACHAYF_002229"
     CDS             4642..5394
                     /locus_tag="ACHAYF_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8579649.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5384..7555
                     /locus_tag="ACHAYF_002230"
                     /pseudo
     CDS             5384..7555
                     /locus_tag="ACHAYF_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7667..9838)
                     /gene="copB"
                     /locus_tag="ACHAYF_002231"
     CDS             complement(7667..9838)
                     /gene="copB"
                     /locus_tag="ACHAYF_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8579650.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9858..12044)
                     /locus_tag="ACHAYF_002232"
     CDS             complement(9858..12044)
                     /locus_tag="ACHAYF_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8579651.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12044..12253)
                     /gene="copZ"
                     /locus_tag="ACHAYF_002233"
     CDS             complement(12044..12253)
                     /gene="copZ"
                     /locus_tag="ACHAYF_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8579652.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12266..12706)
                     /locus_tag="ACHAYF_002234"
     CDS             complement(12266..12706)
                     /locus_tag="ACHAYF_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8579653.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12780..13319)
                     /locus_tag="ACHAYF_002235"
     CDS             complement(12780..13319)
                     /locus_tag="ACHAYF_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8579654.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3771 a         2813 c         2389 g         4455 t
ORIGIN      
        1 aatagttcaa cttttggggt tcagttcaaa gaggggggga ttttttatcg ttgtggaata
       61 tttaaatacc aacgcttgtc atgaaaatct tgtgctccac ccttagtatt tccttctgga
      121 tcgtttgtcg cacgcatcat gacgtatact ttcttattag ggaaatcacg catattgaaa
      181 gatacatgat agccaacatt tcctaaagta ttataagctt gatttacgtc tggtctataa
      241 atcccatcag ctcttactct agctagttct tttccagtat tgtagtccat aatgaaaata
      301 tactcgtatt tataattagc aatgtgccat ccagctacat gcaagtttgc gttttcgatt
      361 tctccgaact gatcaatgtg ggcgtaattt gttccatctg tcagcgtaga atttgcagca
      421 cctgctcgag ttggatcaat tactggttta tcctccgaag tagttggatt atcatccgta
      481 aaaccatgag ccaaatcata ggctagtttt tctttactta cacccatttc agaaagataa
      541 ccgtaaggat ctgtatgatc gccccaaata ttttgtgtta cccataaatg cgatttgatt
      601 cctggttggt tataaggagt gtctaatgtt aatggaatac catatttcat tgctgaatct
      661 cttgccaatt caacgtatac tttatagttc ttttcaaaag tttctttatc atgtgtatgt
      721 tgtaactcga tttgcacagg acttttggct cacgaggttg tcctactcta catcttgctg
      781 atagatatgc tgaaaggaaa acaccaacat tatgttttgc tttttcagct agaacgagac
      841 aagcaggaat acctttagaa gatatcaaag gttttttagg tcataaagat gtttccacta
      901 cacaagtcta tgcccatatt tctcccgaag tgaaaaaaag atcaatgaat caactagaaa
      961 actatataga agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc
     1021 actaaaacta tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa
     1081 tctagcgaaa aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag
     1141 cctggcttgc ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt
     1201 gatgatgatt ttatccatca attgagattg actgttttgc tctcctatga tttcagctat
     1261 gattgtccga ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa
     1321 ctcgctaagc atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc
     1381 taccattcgt tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg
     1441 taatgtttgg gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt
     1501 aataggttct tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag
     1561 gacatctagt tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac
     1621 tttttcacga ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc
     1681 catcggcgat cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc
     1741 caatggattt cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat
     1801 cttttttcca aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc
     1861 tttcgatacc aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg
     1921 gaaaaaaatg atcgatgatc actagatctg cataccgagg cggcttattt cgagtacata
     1981 catgtataga gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc
     2041 ttgcttcttg gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat
     2101 ctaaataccc tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt
     2161 gtgcaagtgc ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa
     2221 atttttctaa tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa
     2281 ttacgtaacg aagtcattct tggtaaagta cttttgtcag ctttgatttg ctttcgctct
     2341 caaattcgct attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc
     2401 gatagtcttg tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca
     2461 agcgcaaaat tgctagataa tttttttctt ttattggcaa aatctgctcc tctctaaata
     2521 gcaattcagg aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg
     2581 ccggatcctt tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat
     2641 catctgcaaa aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc
     2701 tttctgaacc aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc
     2761 actttctcaa tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata
     2821 gcagattttt cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag
     2881 gttcgctata tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgaa
     2941 tgggacagtc gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt
     3001 tcagttcttc agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg
     3061 aaaataaatt ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac
     3121 ctgatttcta aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc
     3181 cctttctatt tatttaaata cgcaaatctt cctcattatt ttttatttta ctcaatttaa
     3241 tcgtattctc atttcaaaac gaatgtactt taaaaaaaca taataagatc actttgcttg
     3301 taggatgtat tcccttccat tacaatgaaa taaatagcga ttttatgaga aggtgatgtg
     3361 aaaatggaag aaaaaataga agaatccaaa caaaaaatcg atcagttgat ataagaatta
     3421 gaaaaagcgg tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca
     3481 ggtggtcatg tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca
     3541 ctagcaaaag ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct
     3601 agtgatattc ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg
     3661 ggaccgatct ttaccacgat cttgttagca gatgagatca atcgtacgac tcctcgtact
     3721 cagtcagcac ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac
     3781 cctctagaca atcatttttt tgtcttagct acgcaaaatc caatcgagta cgaaggaaca
     3841 tatcctttgc cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct
     3901 tcttttgatg acgaactttt gctacttttg gataaattcg aaaaagtgtt ggataactta
     3961 aatgaagtat tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt
     4021 gtcagtcccg aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagtcatgct
     4081 gccatccagc ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct
     4141 tatgctttaa tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct
     4201 tacgtgtttt cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaagaaa
     4261 caaatcattc atactcttgt tgcacaaatt cctattcctg tgaggtagat tgaggtagac
     4321 aatggaaaaa atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat
     4381 tgtgcttttt gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac
     4441 tctttatgta tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac
     4501 ttgtacattg cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc
     4561 aaaaaagaga actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt
     4621 tgcagaaaaa aacaagagat tttgattctt acaaataaac cgcaagaagt ccaattgctt
     4681 tggcagccaa aagaaagagg atgccatgaa acattaactg tcatttttac agcctatgat
     4741 agtctaggac ttttcagaaa aagtgcaaaa agagagatct cttttcctgt gactgttctg
     4801 cctgcttttt gtaagattca agcagaaaaa ttacagagag ttctggaaaa aaaacagcag
     4861 ctgaacgcgt accaaagagg tcttgatttt cgggaacatc gcgcttatcg tccaggtgat
     4921 tctttcaacc gaatcgattg gaaattgtct gctcatactc aagagtggtt ataccgtgaa
     4981 tacgattatc aagaagaaga atgttctcct cttttttgct tttggggttc gcccagtcca
     5041 aaatttgaac ctctgctaga cttgtatttt tctttatggc atttgagaaa agaaaagcag
     5101 ccggaacttc tgatattagg caaccgcgcc tttcatggca aagaccccgg tcatacctat
     5161 tttgcttctt tggaagctgg cgatgagaaa gcttttttcg cacacttgaa aaaatatgca
     5221 aaaaaacaaa tcgtcctctt tgttcctgag aattctcctg aagtctcaga agctattgaa
     5281 acattgtcaa aagatcgaac ggtttatctt gtttatttcg tagaaaaaca actcatggtc
     5341 caagaaaaag acaagatatg ttcattgcca ggaggtgaat ggattgacga ttaaacggga
     5401 aaaatggctg cgtggcgttt actcctttgt gatactaagt gtaggtgctc cgatatttgt
     5461 agtagctaat caattgcaaa acagctacct tcttatccta actattggtg tcctatgcct
     5521 tattgtctgc tgtattccta aaaaatgggt acgtttgctg ctttctttgc cagtaatgat
     5581 aggttgtttg tttttatatt ttccttcgaa gacattttcc atattatgga tcttacagtt
     5641 tttttcgtca gtcacagagg aattcttcca tctatcccaa ggtaaacttt attatttgcc
     5701 agaaaagaca gctttttttc tcataatgtt gctgatctat ttatttatta cattgaccgt
     5761 ggattacgac tattggtacg caccctttct ttctttgatg gcttacttca tgatgcttac
     5821 cgtttttcgg aaaaaagatt tactgtacga aatgattgcg gtagtaactg ttttgttcgt
     5881 gtatctggca gcaaggcaat ttttctccat ccggttgttg tcgggaaaca ctcgattcca
     5941 gtcattgact acagttctgc ttatttgttt cttaactttt agcgctatct tgcctttata
     6001 tcttccgaaa gtacaccaat ctctcgagga aacgagtgaa ccgatccgag aatatcttaa
     6061 tgatgaaggg ctttatgaca cactccatga gtatcaatta accggaagtg caagaacagg
     6121 cttcagtgag aatgatgaac agcttggtgg acctttatat gataatggcg aagttgtttt
     6181 tacagcgaac caaaaaggaa atggctattg gaagatcgaa aataaaaatc attacaccgg
     6241 ttctggatgg atagaagatc aggacgcacc catccaacca atccaagaat tccctgttga
     6301 catcacgagc taccaaaccg ttatagaaga caaagaaaac attacattga ccctaaaaaa
     6361 acaagttttc ttaccttatc cttatggaaa tactaccttg actgattttt cattagctag
     6421 tggacaaacg ctttactatt tgcctgaaag ccagcgattc attcctgtcc cgaatgaatc
     6481 gacacgttct gcaactttac aaacagaaag gttagttttt acgagagaag aattaaaaag
     6541 cagtcagtta aaagctgatg atttagaaaa tcagcagaca aatcttcaat tgccaaaaag
     6601 tttgcctaaa cgtattgagg acttggcggt cgaattgacc aaaaatgaag caactttaat
     6661 tcgtaaagtc gaagctgtct aacagtattt aaaaacaagc ggaaatttcc gctattccaa
     6721 aacggatacc gggcatactc caaaagaaca cgattatgtg gattatttcc tctttgattc
     6781 aaaagtcggc tattgtgaca acttttcaac ttctatggtc gtccttcttc gaacacttgg
     6841 tatcccagct cgctggacaa aaggttttac aaatgggctc agaacaggca cagataatca
     6901 agggaatgag gaatacagca ttctcaatag tcatgctcat tcttggccag aggtatactt
     6961 tcctggattt ggctggcttc catttgaacc aaccccttca tttgccaatc caatgggttc
     7021 acaaccagtt gctgaaacaa ccgaaaccgt ccagcctgct atcgaacaac caaatgaaag
     7081 cgaaaatatg gcagaaacta tttccagtga gagatctgag gtcattccat cagaagaaac
     7141 aacactcaca ccagcttata aaatcgtctt attcagcctt ggagtgtgtt tatccgtctt
     7201 gttgttcttt ctattgttat tcagaaaatc tctgcgctgg acaagttatc ttatctatct
     7261 tcattttttt gcttctacta atacgattgc atatcaaaaa cttcttaacc ctttggaaac
     7321 attggtaccg cgcaaacccg cagaatcttt gcaggaatat agcctgcgaa tagaacaaga
     7381 attcaagcta gatctgcgtt tcattcgatt aactactcaa tatgaagcag aaatatatgg
     7441 acgacaagca gatccggtaa aattcgataa aaaaacctat ttagaaataa cgcatataat
     7501 aagaaaaatg gtatacaaaa aaattctgac aaaagggagc cgcttcaaaa aataagataa
     7561 aatttctgga gaatctactt ctggaatgcc gagaaccagc tgattgagta tcgattactc
     7621 gaaaaaaagg ttgtgacacg ttttttgtca caaccttttt tcattattat ctgattttca
     7681 atgtcaaagc atttagagct acaactaccg tacttaacga catcaagaca gcaccaactg
     7741 ctggacttaa tatgatacca attggcgcaa gaattccggc tgctagcgga attgcaagaa
     7801 tattgtaacc tgccccccac cagaggttct ggatcatttt ctttcgcgtt tgcttggcta
     7861 gatcaagaaa atgcaaaata tcttttggat cgctgtttgt caatacgaca tccgcagaat
     7921 cgattgcgat atctgtccct gcaccgattg ccatcccgat tgaagcacgg gctaaacttg
     7981 gcgcatcatt gatcccatca cccaccatga ttactttttt cccttggtca aggtatttct
     8041 ggacaacagc ttctttatca tctggtaata aaccgccata atattcttcg atccctaaat
     8101 aatctgctac tgcttgggca gcgtttttat tgtcaccagt aagcatgaca ggagtgattt
     8161 ttctttcttt caatgttcgg ataaattctt ttgcttctgt ttttactacg tcacctaaag
     8221 caacgattgc agctagatga ttttctaaaa tcaggaagct gatggtattt ccttgttctt
     8281 ggtaaggttt caataatgct tgttctacgt ttaatccgag acgctcaact tctttttcat
     8341 taactatttt cacttgttgt tttttgactg ttgcctctag cccgactcct gaaagatttt
     8401 ttagattttc agcttgataa ggcttgattt catgttcttt caaataattc ataatgccaa
     8461 cagccaatgg atgattggca ttcgcttcca aggctcctag atattgaagg atttcatttt
     8521 tattgaacgc atcatcaagt acttcgacac ccgttacagt aaattttcct tctgttagtg
     8581 ttcctgtttt gtccagcatg atcacatcta gatcattggc ttgctccagt gcattgcggt
     8641 ttttcaaaag taatccgttt ttagctgcta ttgatgtgga acgagcaaca actaatggga
     8701 tagctaaacc tagagcatga ggacaggcga tgataaagac tgttaccata cgttctaatg
     8761 cattaggaag atctgtcaag aacaaccagg cgatgaatgc caggataccc acaattaatg
     8821 cgacataaaa cagccatttt gccactttgt cagataatga ctctagcttg gatttttctc
     8881 cttgtgcttt tcgcaccatt tccataacct tcgctagata accattttca ccagttccag
     8941 ttacttcgat ttcgatcgtt ccatccccat tgatagaacc gccaatcact ttgtcattgg
     9001 cttgtttttt gattcctctt gattcgccag tcacagctga ttcgtcaaca attgtttcac
     9061 ctttcaagat tacaccatct gttggcattt tatcaccaga ccggacgatc aaacgatcac
     9121 cttcgtttac ttctttcaat gaaactgtct cttcttttcc atctttcgtc aagcgtttca
     9181 ctgattctgg cagtaattct gctagttttt gtaaagcatt gctggcattc gacacagcat
     9241 tcatttcaat ccagtgacct agtagcataa taacgatcaa agtagccaat tcccagaaga
     9301 aatccatgac gtgtgtatgt ggattgagca gatttgcgat aaacgaatag acactataaa
     9361 tatatgcaac tgtaatcccc atcgcgatca gtgtcatcat agcaggactt ttttgtttca
     9421 attccatctt agctccactt aaaaatggct gaccgccata gataaatagt atagttgcta
     9481 agatcaatac gagccaatca gaaccaggaa acgttacttg aaatggaaat tccattccca
     9541 tcattggtga aaaaagaatg attgggatag ctaggataag cgatagccaa aatttttgtt
     9601 tgaaattccc catgtgcatg gaatgatcca tgccgccgtg ttcgtgatcc atgtgcatcg
     9661 aatggtccat ttgtttgctg tttgctatat ctccatggtt atgtcccatg tgcatggaat
     9721 gatccatctg actatggtca tgtttcatct ccatctgttt ttcatgagat tctgtacttt
     9781 ctttgtttac tacttctttt ttatgctcat gttttttctc catatcgttc ccattcattt
     9841 ttactccatc ccttctctta ctttttcatg tgtcgattta agcacaggga gttcaacaat
     9901 acactgacgg aactaaacgc catcgctcct cctgcaatga ttggattcaa aaagcctagt
     9961 gcggcaaaag gaataccaat cgtattatag acaaaagccc agaaaagatt ttgtttgatt
    10021 tttctcaatg tcaacctaga caactcgatt gtttgtgcaa tggatgccaa actgctattc
    10081 atcagcgtca catcggctgt ttccatcgca atgtccgttc cacttcccat tgccataccg
    10141 acatcagcta atgctaaggc cggtgcatca ttgattccat cgcctgccat accgactttt
    10201 ttgccatctt tctgaagttt ttctacgtag gctgctttat cctctggaag tacttcagca
    10261 aagatatgct ttggatcaat accaacttgt ttaccaatcg tttcggctgc cagtttgttg
    10321 tctcctgtca gcataaaaac atctaatcct ttattctgga gctgcttgat tgcttgtttg
    10381 acttctagtt tgatttgatc agccacggca atcaacccga tcacttgctt ttcatcagct
    10441 aaaaacatga ctgtttttcc ctgctgttct aattctaacg cgtgttcttg gtattcatca
    10501 aatgataggt tcaattcaat taatcgttta cgtgtacccg caaaatagcg tactccgtta
    10561 atcgtaccgc tgattccagc acctgggtgg gctgtaaaat caatcatatc gtatgttgct
    10621 gcttcttgca gttttccgta ttccacgatt gctttaccta gtggatgttc agaagcttgt
    10681 tctaaagtat aaaaaataga taggacttct ttagaaccga ctagatctgt aactttgggc
    10741 tttccttctg taatcgttcc agttttatcc aaaacaatgc tgtctaagtg agcagctgct
    10801 tctaatgctt ctccgccttt aattaaaatt ccatttcgtg caccaacacc cgttcctacc
    10861 ataatagctg ttggagtcgc taaacctaat gcacatggac acgcaatgac taatacagag
    10921 acactatgga tcaatgcgag ttgccaatca ccagtgatca aacctgtagc tatgagcgtg
    10981 ataaacgcga tgactaatac gatcggaaca aaaatccctg agatccgatc tgcgattttt
    11041 tggattgggg ctttgctccc ttgagcatct tccaccattt gaatgatttg cgctaatact
    11101 gtctgcttgc cgacttgtgt aacttccatg tgaagtagac cattcgtatt gattgtcccg
    11161 ccaaagagcg tgtcatccgg attcttttct acaggaagat tttcaccagt aagcatactt
    11221 tcatctatcg cactgcttcc tgaaataatt cgaccatctg ctggaatctg ctcaccagga
    11281 cggatgacta gttgatcacc aacgactact tcttcaatcg ctaacgtttc ttctttcccg
    11341 tttcttatca cttgggcggt ttttgtttga agagacatca attgtttgat tgcattacct
    11401 gttttcgttt ttgctgtatg ttccaaatat ttgccgagca aaatcagcgt gatgatcata
    11461 ctgctgcttt caaaataaag atcttggggg tgtccacgga aaaatccgtt gtaaatgctc
    11521 aatgcaaagg cagccgatgt tccaattgct accaatacat ccatatttgg tgctttcgtc
    11581 ttgattgcat ggtacgcacc tttataaaaa cgagcaccga cataaaattg gacaggagca
    11641 gacagcacta actggacgat tggaaaatga aaaaagtgaa caatcgccgc atggcttcct
    11701 aacatcatag caatcattga caacatcaac ggcaaagtca aaaccgtact caatataaga
    11761 tcgaatagca tttttttcaa atacgcttgc ttttcttctg caattttttg tttgtgggct
    11821 tcatcgtaca aaattgcacc atagccgatg ttttctacgc tttggattaa tttttcagtg
    11881 gtcgtccctt catatttgac acttgctttt tcagtggcca agttgactgt ggcattgacc
    11941 acaccaggtt gtccgttcag ctctttttcg atcctagcag aacaatttgc acaagtcata
    12001 cctgtaatga cgaacgtatc cattttggtt tctgctgttg ccatcaaatc acctctgctt
    12061 tataacccaa tccattgatt gcttgacaaa tcttttcgga ttgaactttt gtttcatcaa
    12121 acttgaccgt accgtttgct tttttcaaat ttactttcac tttttgcacg ccgtcaagtg
    12181 cgcttactgt ttcttctact cttgctgcac aatgattgca gctcatacct tcgattgaaa
    12241 attgttgttt cattttttct tcctcctatt gatgttgttt acattcacat tgacctggga
    12301 tacagttaca ttcgatcgta tctactggtt gcttttgttc cagttgtttc aaaatctcat
    12361 tgatatcttc ttgagtcaaa gtggcttctt ctactagatc cgcaatcgtc tctccaatcc
    12421 tttttgcaca gatatgggaa aaaagatttt ccgtcgcact gcgaactgtt tccatctccg
    12481 agacagctgg ataataaatg aatttttttc cttcttgttc tgtccagatc acttcttttt
    12541 ttaccaaacg acccagcaaa gttttgatcg ttgctggttt ccaatccatc gaatcagcta
    12601 agatctgcgt gatttcttgg gctgtcgtct gtccaagtgt ccaaataaca cgcatgatct
    12661 cccactctga atcactgatc tttactggcg ttttgacttt ttccatttat ttcacacctt
    12721 tcgtttacaa atgtaatcat tatcttatga atacagatta cacttgtaaa caaaaactgt
    12781 caattatttt tgctctctat tttcaattta gttaaaaatt cttgtttctc tcgtcttgtt
    12841 ttaaggatga tcagtttatt tcttgcatta gctacttgga tcagctgctg tattttcggt
    12901 tcattgtttt cttcaaaatg ccagataaaa gataaaaatt ctaagtattc cctgtttagt
    12961 ttctccgaaa atgattcagg catatctggc cgagattttt tgtttatctt tctttttaga
    13021 gaacgccaaa ctactcggta gatggcgaga taacgcggca ctttaagcca aatgatctta
    13081 tctgcttctt taatcctttc ctctaatgta gaagtataat ttccttcaac gatccatgac
    13141 ggattagata ccataaacaa ttgctgttct tgcaaaaacc acttttttgc ctgaatactg
    13201 tagtctgttg tatgccataa aaggtctagc ggcagtagcg gaaagtcggt gatttctttg
    13261 atcttccttg cgagggtcga ttttcccgac ccactttgtc caatcacgat gatcttcatt
    13321 cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac gccgcaaaaa
    13381 aacaagggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaata
//
LOCUS       DAYYCQ010000077        13367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000077 DAYYCQ010000000
VERSION     DAYYCQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.077"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACHAYF_002236"
     CDS             complement(<1..244)
                     /locus_tag="ACHAYF_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8579655.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAF"
     gene            complement(723..1442)
                     /locus_tag="ACHAYF_002237"
     CDS             complement(723..1442)
                     /locus_tag="ACHAYF_002237"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8579656.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1491..3137)
                     /locus_tag="ACHAYF_002238"
     CDS             complement(1491..3137)
                     /locus_tag="ACHAYF_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8579657.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3316..3906
                     /locus_tag="ACHAYF_002239"
     CDS             3316..3906
                     /locus_tag="ACHAYF_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8579658.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3998..5503
                     /locus_tag="ACHAYF_002240"
     CDS             3998..5503
                     /locus_tag="ACHAYF_002240"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8579659.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5587..6939)
                     /gene="celB"
                     /locus_tag="ACHAYF_002241"
     CDS             complement(5587..6939)
                     /gene="celB"
                     /locus_tag="ACHAYF_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8579660.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6939..7457)
                     /locus_tag="ACHAYF_002242"
     CDS             complement(6939..7457)
                     /locus_tag="ACHAYF_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579661.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7473..7799)
                     /locus_tag="ACHAYF_002243"
     CDS             complement(7473..7799)
                     /locus_tag="ACHAYF_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8579662.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7812..9791)
                     /locus_tag="ACHAYF_002244"
     CDS             complement(7812..9791)
                     /locus_tag="ACHAYF_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8579663.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9812..10126)
                     /locus_tag="ACHAYF_002245"
     CDS             complement(9812..10126)
                     /locus_tag="ACHAYF_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8579664.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10294..10587
                     /locus_tag="ACHAYF_002246"
     CDS             10294..10587
                     /locus_tag="ACHAYF_002246"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8579665.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10665..11777)
                     /locus_tag="ACHAYF_002247"
     CDS             complement(10665..11777)
                     /locus_tag="ACHAYF_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8579666.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11930..12778
                     /locus_tag="ACHAYF_002248"
                     /pseudo
     CDS             11930..12778
                     /locus_tag="ACHAYF_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13131..>13367)
                     /locus_tag="ACHAYF_002249"
     CDS             complement(13131..>13367)
                     /locus_tag="ACHAYF_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8579667.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4245 a         2679 c         2273 g         4170 t
ORIGIN      
        1 gaaatgcttg atccgaagaa aataaaaatt tgaaattttc ccaacccacc caaggacttt
       61 ccctcaagct agccaagaac ccatctgctg aaatatgaaa atttttgaat gctacgacat
      121 tcgccattac aggtatgtaa aagaaaaaaa tcatccaaag catacccggt acagccatca
      181 ggacaagtgc tttataacgc catagattca taaaaaaatg attgaacccc tttcgttttt
      241 tcatctaaaa acccctttcg attcattcgc aacgtaatta tagcgctttc aataatgaga
      301 ataaataaac aaagtatagg gaaaatgaaa caaagtatag gagaggttgt gaaatcagcg
      361 ttttggcctg agtattagag aaaaaaacgg gaaaatagtt cctcatattt tgccgttttt
      421 tgagctaata ccgagggtca gctgattgaa caccgtttat cagaggttgt gaattcagcg
      481 ttttggcctg agtattaggg agatcgtgtc cttactatct aactctgagt cacaaggaac
      541 aatggggact gtaacaaaag tcttgtcaca gtcccttatt ccgaacaaac ggtgttccag
      601 aagtagcttc tacggaaata agccgaaatt tcacaaaaaa gccatccgaa agtcagacgg
      661 acaagtctgc ttttcagatg gcgctacaac tactctgctt attcttttag ctatccattc
      721 aattattttt ctctcacttg ttctagctct tctttggtca tgggtcgata ttgcccttta
      781 ttcaaatctt taggcagcca aaattttccc atacggattc tttttaaata taagacttct
      841 tttcctactg ctttgaccat tcgcttaact tggtgaaact ttccttcatg caaaatcaga
      901 cggataattg aagtttctgt ctcttgatct acttgatcaa tttggagttt tgccggcaat
      961 gtctgctcat cgccatcaat aatcagtcct tctgcaaata aacggatatc ttcttctgtc
     1021 attataccgc taacctcagc tagatattct ttctccacat gttttttagg tgatagtaac
     1081 tgatgggcaa acaccccgtc attcgttaaa atcaagaggc cctctgtgtc tttatctaat
     1141 cggccgactg gaaaaagatc ttcccgataa tcctggtctt ccaataaatc gattacggtt
     1201 tgatcatgat tatcctgagt agcagaaatg actcctgcag gtttatgcaa aatgtagtaa
     1261 aaatcttttt gatacgtaat gatttctcct aaataagtaa ccgcatcttc gaattcattt
     1321 acttgcgctt tatcgctttt caccacttgt ccattgactt cgatcgttcc ttttttgatc
     1381 aatcctttga cttctttgcg gctgccaagt cccatatctg ctaaaaactt atctaaacgc
     1441 atgcgtttcc cccatttcaa aagagcatca atctagctct tctgattcac ttattttatt
     1501 tttaatttat gtcgtaaacg cgctattcct ggtcctagta gtctttctgc caagcggatt
     1561 ttcaaagcta aataaccata cactaaagcc cctatacctg ctactacaag aatcaacaat
     1621 agagactgga atttgctatc ttcgtttaaa aaagcaccaa aaatcatctt tgtgatccac
     1681 gaagccaaaa gcataatcaa ggtaagaata aagatcagca atgtcctgcg ccagacaaaa
     1741 cttggtttaa agcgggctac ttcatggatt ctctttaaga tcaaggtaca agacacagca
     1801 aatccgatca ttgtcgccaa taaagggccg tatacttcaa ataaacgaat ggctgggtat
     1861 tgaagaagga gtttcactag aaacccaata accaaataat taattgccgc cttgttttca
     1921 aacattcctt gcagcatgtt ggaaactagc atatatagtc ctaggaagag tcctacaaaa
     1981 cttgcttgga tcagtacatt acttcctaga gaatccggtg tataaaataa tgtatttagc
     2041 ggataggcaa gtaacataac accaaatgtc gccggaaaca taaaaaatga aaacaactgt
     2101 aaatttccgc ttgtcaattt tgccagacct actcgatctt tcaacgtaac agcttctgta
     2161 atcaacggta acccagtaga cgcgatcgat gttgccaatg caataactac catcgtcaac
     2221 ttatctggat tggcactaaa aatcgaaaaa agatccagta actgtgcatt ggaatactcg
     2281 gtggatgcag acatgatctt cataaaagtg aactgatcga ctaatttgaa gatcgttacc
     2341 cctgaaccca caataataaa cggaatcgct tctttaacgg tatctaacaa caattccttc
     2401 gttgcaatat ttattttgtt ttcgctgtag tgaataaacg ccgatgtata cgcttgatgt
     2461 ttcttcaaaa aatacaaaag gacaagaata cttgctagca taccgataaa tgccgcgaaa
     2521 gttgattgcg tgaccgccgt cacatagtca ccatccatga ctttcatgat gatgaatgta
     2581 gccaatagca tataaaaaat acgtgccacc tgttctacaa tctgcgacag cgcataaggc
     2641 atcatttctt gattgccttg aaaataccct cgtatcacac tcatacaagg aaaaaccaat
     2701 acagcgatac tcaaagaacg catcgttgga atcagttctt ctccgccacc agaagcatga
     2761 gctagccaag gagaggcgat gtacatgaac aaagcaaaca acaggcctag cccgcccatc
     2821 atctgtaatg cacggataaa taatcgtcgt gaagtgccat attcattcaa tgaattatag
     2881 cgtgcagttt gttttgcaat cgcagcaggt attcctgctg tagagatcag tagaaacaat
     2941 gcatatatat tgtatcccat attgaataat ccattggcag ccttagcatt ttcgcccatc
     3001 caagcatacc agggaatgat atagacagcg cctagcaatc ttgaaatgat attactggcg
     3061 gtcatccaag cagagcctcg tgccattttt tcttgcggtg ttaattgatc taatgctggt
     3121 tttctttgat tatccatcgg tcagattcca actttctaca aaacattact atcattttaa
     3181 ttggtaaaac agaaactggc aatcgatttt atataacttt tataagaaca attgtttttt
     3241 tgaattcggt ttcgtttttt tggtaaagta aagaaatata cagcctttta tggctaacat
     3301 ttataggaga tatcaatgac acaagaaata aaaaaactac ccgttatatt tttaggtctt
     3361 ttctttatca cagttggatt gaattggttc ttgctccctc acgatatagc ttccgccgga
     3421 gtgggcgcca tcggacattt agttgaaacg gccttttcta tccaacgctc agttactgtt
     3481 tggagcatca atcttatgat gctgcttcta gccgctgtcc tattaggaaa atcagtattt
     3541 tttaagactg tagcgggaag tctgcttttt cctgttgttt tagagattgt tccaaaagtg
     3601 gaaatgatct cctcacattt cttttcactg atttcaggaa gtcttttgtt cagtcttggg
     3661 gtttacacat tgtacacagt cggggcctca aatggcggta taacgatacc gccgattatt
     3721 ttccacaaat tttttcggct tccaatgccg caaggacttt tacttaccaa tagtcttatc
     3781 gtctttttga attattatgt ttttggattg ttggagacac tattcgttct cctatctatt
     3841 acagtcagtt cattgtttat gaaaatactc atccgcttgt cccctgtgtc aagaaaattt
     3901 gaataaagct ggattttgtc atagcggaaa caaacgatat attaaaaaga gtgtgctata
     3961 ataaaccaag aaattgaata aagcgaggga tcaatcaatg agcttctctt tagaagacat
     4021 ccgtcattta ctattaaaag aaaatctatt aaaagaattt gtttcaacaa gaggctggca
     4081 tcttgatgta ccagaaaaag ctgaatttct tcagttaagt tatgattcaa gaaaagctga
     4141 tgcttctact ttgtttttct gtaaaggaat gaatttcaaa gaagaatatt tggattcagc
     4201 aatcgaacag ggtattcaat attatgtatc agaacaacca tacgaaaatt cagcagtagg
     4261 aatcatcgta acggatatcc gtaaagcaat ggcgcttctt gctatggctt tttacgacta
     4321 tccgcaaaat aaattgaaag tgatcggatt cactggaaca aaaggtaaaa caactgctgc
     4381 gtactttaca aaagcaatct tggatcacac aacaaataaa aaaacagcat tgctttctac
     4441 tatgaataca acgttagatg gcaaaacata tttcaaatcc catttgacaa cgcctgagtc
     4501 attagattta tatcgtatga tgtcagaagc agttgaaaat ggtatgactc atctagtgat
     4561 ggaagtttct tcacaagctt acaaaacaca acgcgtatac ggcttgactt tagatgtcgg
     4621 aatcttcttg aatatctcgc ctgaccatat cagtccgatc gaacatccga catttgatga
     4681 ctatttctat tgcaagcgac aattgatctt gaattcaaaa acagttgttt taaatcatga
     4741 aagtgactat tttgatttac ttagggaaac agccgatcta ttccagattc caacgatcac
     4801 ttatggtcgt tcggaagaaa gcaattatca agtgatccgt tcagataaag gtactcacgg
     4861 ttttacttta tcttctcatg aagatcgctt agctatctgc gatactgctt atgatatttt
     4921 gctggctgga ggattcaacc aagaaaatgc agccagtgcg atcattgccg cagcactaac
     4981 aggtgcctcc agtcaagatg cacaagacgg attaaaagaa gctcgtgtac caggccgaat
     5041 ggatcaattg atccaatcca atggagcgca tgtctatgtg gactatgcac ataattatct
     5101 aagcttgaaa acattgctgg aatttgccaa aaatgaacat ccagatggcc gtgtaattgt
     5161 tgtattaggc agcccaggaa acaaagcaat ctcccgtcga catgattttg gaaaagtgct
     5221 atcagaaaca gctgacatcg cctttttgac ggcagatgac ccagcatttg aagatccgaa
     5281 aaaaatcgcg gaagaaatca atgaagcaat tactaatcca gatttgatcg ttcattatga
     5341 aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa agcgctccgc aagattcagt
     5401 cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc aatggtgtag atgaacctta
     5461 tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa taatggtatt tatatactag
     5521 tggtttccca tttctaagaa aaaaagggac tgtgattttt tgtcacagtt ccttttttct
     5581 tagaaatcat tttaactttt cactagctaa ttctgctgcc ttttctcttt cttcatttaa
     5641 taatttatta tcatagaatt tgataaatgg gaaccagata atgaatgcga tgattgcaca
     5701 aatgactgct aacacagcag ctttccaatc accaccacta ccaacaaatg ctccgatccc
     5761 gactggtgaa ggccacggca tctgtgcaag catcggtttg acgatttcca gccgaatggc
     5821 aaaataagcg agtgtagcag aagccatcgg agctaagaaa aacggaaccg ctaaataggg
     5881 attgtagacg attggcatcc cgaaaatgat tggttcattg atattgaata atcctggtac
     5941 aagtgaggct tttcctaaca ctttcagctg atcagatttc gcgaggaatg caataaagat
     6001 gatcaatcct aaagtcgcac ctgatccacc aacggttaca taagaattgt tgaattctcc
     6061 tgccaacgga taatttgcgc cttgtgcgtt agctgccata ttagctaata cgatcggggt
     6121 caagaaagaa gtgatgatat tcgctccatg gatccctacg atccacaatg catggatcaa
     6181 gaaataaatc accatgacac caagccatgt attcgtcaac tgcgtaacaa acccaaatgg
     6241 gatagcaatc attttgaaga tatccgttcc tagtgcaact aataatccat tgatgatcat
     6301 caccacaaac gcaatgacaa atgttgggat cagagcagtg aaagaacgag aaactcctgg
     6361 cggaacaaca tctggcattt ttatgaccca atttctcttg acacacatac aatacaattc
     6421 gaccgcaata atcgacataa taatagctgt aaagattcct gaagttccta aacgttcgac
     6481 aaaagagccc atcctaatac catttactac agtttctgtc tcattgatag aagcaatcaa
     6541 gctgatttta ccatcagtca tgatcaattc agggatacac ataaagaatg caaaaacaga
     6601 tagtaatgcc cccgtgagcg gattaacatt taatttttgt tcatctcgtt caattgacgt
     6661 taattcataa gcaaacacaa tggcaaagta aatagataaa atccccatcg tcattgtatt
     6721 gacgatcatg tacaagtcac taactttgaa aaaagttgca ttaaaaaatc cttctaaaaa
     6781 tgggaacggt aatggcaaaa tatttaaaac caaaaacatc gagcctacaa tcgtgaaagg
     6841 aatagaagcc atccccgctg ccatgacagc ccgaacgatt ttaagttgcg caattttccc
     6901 cataggtccc atcaggtatt tctgcaaaaa atcaaacatt agttattctc ctttcctaaa
     6961 tataacgctt tttcatattg tttaatacgg atatactgtt ggtcttggtt gagtgaaatt
     7021 ttctttaaac gatactgtat aaatgcactg gcaaaaatac cgcttacaac aagaatgaaa
     7081 acaaataact gtcctccatt gttatttgcc ataaaaggat acaagaatgt aaatcctgag
     7141 gtaaacaaca ctggaatcac taataaatta aagacactct ggaacaaaaa ataccacttt
     7201 gtccatggaa cgttcgtttg gtgttttcga taaagcttta cttgttctcc tactgcaggc
     7261 aaaatagcta aaatcagaat catcggtaaa ctacttccta aacttttagc tgatagaagc
     7321 agaatcatcc attgcatgtt catgaaaaag aaaaaggcag tcaggtaacg tacgattaga
     7381 taacgattga aatacatgtt tttcaaacta agttgctttt tttctaattc gattttcctt
     7441 tttactgtat cctgcacaat gatccttcct ttctatttat ttttttgata caaataattc
     7501 atttcctgtg caacttccaa taatgtcatc gtcgtcatca gatgatcttg tgcatggacc
     7561 atgataattt ccatttctat ttttgtcccg ctggcatatt ctttcagaag ttgagtttgt
     7621 gactgatgcg catgaagaat ctcttcattt gcttgtcgca acttctcttc tacttcttca
     7681 aacatctctt tacgcatcga gcgaaatgct tcatgtatca gtgtccgtgc atttccgcta
     7741 tggaaaatga tatcaaaaga agtcacttgt agtgtttcac tgtttatttt ttcagccatc
     7801 tgttttcctc cttattcgat cagtttgata aatttactgc tgaaattctc aaaaataggt
     7861 tcagctaaga tttcctgctg tacttttagc tggtcaacta acctaacgat tgctttagtc
     7921 acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa ctaaaaaaac aaaatgaatt
     7981 tcttgatatt gatcccactg catcccagaa ggaatcaatg caacggctac ctttgtttcg
     8041 attccaactg gaacagcggg atgtgggact gccactgtat cactgaatat gatttgtccc
     8101 atattttcac gttgctggat ctgctttcgc atttctctta tatatccttc ttcttcattt
     8161 atgctcagct tttgcagcaa atcatgaaga atttcttcct ttttcggttc gtacgtataa
     8221 gtcttgaaaa atttttcaga caactgttcg gtgtaaatct gctttttttg ctgtagagaa
     8281 aatgctggta tcggtctatc tgaggaatga tagggtatag attctccaac tacctttcgt
     8341 attttacgaa catcatcttc attcaaaaaa atactgacat gtaaaactgg aattttgaaa
     8401 aacattgtcg aaagatcaat agaagaaata atcaggtcga ttccttttaa agttgtttca
     8461 ttgatctcat aatagccttt tacacttacc actgtaatat ttttaccgaa ttcactgact
     8521 acgcgattct ttaatagttg ggcactgccg taacccgttg cacagataat caatgcatgt
     8581 agtttatgcg cagctctttc tttttccaat gccgccatga gatgcaaagc caaataggcc
     8641 cattcatctt cattgatggt atagtttttc aaagccggca tgttgctgaa atgccttttt
     8701 actgcgtcaa agactttggg attttctttc tttatctcct cagtcaatgg gttctcaatg
     8761 cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat gatcgagtag tccattgaaa
     8821 agctggagat cctctactaa tgaaaaatga aattcctgag acaatctttt cagaactttc
     8881 gctaattcct ttgatagttc ctgatcattt tgattttctt tgtgattcga cttagccatg
     8941 agatgtaatg tcaaataagc aatttcttct tcgggaaacg atacacctgc gattacctca
     9001 atacgttgaa cgatccgctg cgccacttga tactctactc gcttagaaac ttcttgttcg
     9061 atgcctaatt ccttgatttc aaacccttct cggagccgtt tgatccccaa agccaagtgt
     9121 aaagtcagat tctgtatgat aaaatccgaa acttttaact tagcttctct tgtttcatcg
     9181 agaatgataa tgactagctc ttcaaaactg atgtctggga aaaaacggct ttgccctaaa
     9241 tattctttca atgaatttcc ataatggttt ccaaaaaaag tatccatgat aaaatgccgt
     9301 cgatttcttt cttccccttc gatccatgtc ccttgaccat gcttagaaac gatttttagt
     9361 gaatatggaa tcaatcgctc cttgatttct tgcatgtcct ttgtcaaact ggaacgactg
     9421 atataaagct cttctgccaa atcatccata aaaagaatgg catcttccaa aagcaattgg
     9481 ttcaatatat acttttgtcg atcttcggac tcataaaatc cttgcggttc tctagcatat
     9541 ccaggtacta tttcttgccg actcaaaaac aagtcaaatg tcagtctttg taaaatatgc
     9601 agttggtagc cataacctgg tttagcaatg atcttacctc cgtttttttc tactaactct
     9661 ttgatccgct gcaagtaatt acggattgtc cgatcagata ataacaattc agaagctaaa
     9721 cgttgactgg tgacatactc cttcttgtga tcaatcaaat atctcaacag caatttttcc
     9781 ttctgtttca ttatcatcca ctcctattcc atcatatgtt ctcttcgatc acctctgcta
     9841 acttctggat gcctgtaggg attgggacat acgcttggaa agggatacta acaacaggct
     9901 ttccaacaga ttctcctaat tttttgaatt tatccaaaaa catcgtcgtt tgcgggctga
     9961 tcagaaataa atcaaaatca ctattcttaa tcatattatc gccttctgta gctgaaactg
    10021 catctacgag tacctcttct cttttttgct taaagaaatc cgttgtcttt gaggccatca
    10081 tcgaggatga cattcctgcc gcacaaataa tcaatgcttt tttcataaat caatcactcc
    10141 ttttattttt ataaaccaag catatcttgt aagcgttttc tattcaaaat atatatttcc
    10201 gtagcattgc ggaaaaaatg tgtatcatat cttgaataat aatttctggc accgtgatat
    10261 actagtgata gaattttttc aggaggggat ttagtgagta tcacagtgaa tattttgtat
    10321 acaagtacaa atggacaagc cagaaaattt gcagaagaaa tggtcgaaag aaacattgtc
    10381 aaagcgattc gtgcagaaga aggaaacgag aaatatgact attactttcc actagaagat
    10441 ccagaatctc ttttactgat cgaccgttgg aaagatgaag aagcaatcga aaagcatcac
    10501 aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa agtataaatt aaaaatgaaa
    10561 gtcgaacgtt tcactgattt accgtaaaaa gatacacatg gaatgatagc aatttatcat
    10621 ccatgtgtat cttcactttt tccttatttt ttattcgatc tcatctaatg attcaaaccg
    10681 tgtttttctc ttatttttcg gataagtatt tcagtcaccc caatatacat gattcctaaa
    10741 cataccccaa ggatattctc aaaaatgcgg atcgttactg ctcctggaac cccgaataaa
    10801 ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg ttttatatct ataggtagaa
    10861 caaacaccta aagctagacc acctaatata ccgacccagt taaaaggaat aaattggctg
    10921 ataccaataa ataaaacaca tccaatcaat gagccaatga ttcggtctac tgctcgttct
    10981 ttgaaaccaa tcgacatcaa tccatagcta gatacaatag aagaaaatgc aaaccctgcc
    11041 cacatcaatc gctgaaaagg gacatactct cctgcaacga tcaaaagact aatgccaaat
    11101 gcatagatga gcatccaaat tttttcctgc gaatacatgc ctttcaaaaa aatctcttta
    11161 aacaagcttt tgtctttgtt cttttctcga tgttttctgt aaaggagaac tgaaaaccaa
    11221 caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga aattcttgtt caacgaatct
    11281 ttaggtaacg aataaacaat aaagagatat gaaaagccgt acaaagaagc attccccatt
    11341 ttgggatcac tagcggttgc cattaatatg ctgcttagta aaataaaatg aatcaacatg
    11401 cccataactg accatttgat cagctggaca tacggtgcta ctaatagtga gaacatgacg
    11461 attgctaaac ctatgatcga atgggaaatc ttataaccaa agtcaacaaa acgaatactc
    11521 atcaacatac agaataaaac cacagcgtat ggtttatgct tcgattcaaa taatgttgta
    11581 attataatga taaaacaaat ggcaaacaat acgatgaata atgatcgtaa aaacaatgaa
    11641 caaatataca tgcacttttc ttttttagaa gctgcttctc gtattttttg ttttaaaata
    11701 aacggatcta gttgtagaga ctgataaaaa tccacctgtt gtcgtccttt ctttttactt
    11761 ttatttttta cagtcatcta tgacaggttg taaatactat ggaacatcat atggcgttgt
    11821 cattttcttg tcaactattt ttcctattca tgtttttata aaaaaacata tcgttttctt
    11881 gtatgattaa atcaccacaa aataaacaac tgagagaacg atatgcttta tgaatggttc
    11941 tatcaaaaaa attctgagat caatagacaa aaatttgatg attacagagg tctcttacga
    12001 aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt ctcttccctg ctccatgtgt
    12061 ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg aaattaatgg tggtgaaaaa
    12121 caagaagaag gaaaccattg tccactttga ataatataat catatgccaa tggttatacg
    12181 cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc cattggaacg ctcaaagtta
    12241 ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga cataaaatca cttctttact
    12301 gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt taggtatctt tgacaaccgt
    12361 tattcgcaca ttgaaagagt tgagaagcta tttaccaaag tcttccaaaa gaattctgtc
    12421 ttgagtgttg agggttgtga atttcgttct cacgtttccg ttgaggataa actgagtttt
    12481 atttccgcgg atggcgagac agaaaaatta atcgagaaaa tttacctaca caaaaaattc
    12541 ctcgattaac aaactatttc cttagctgca ctaatcctaa agaaatagaa ttcttagtta
    12601 cagacatgaa cgccacctac tttcagctca ccaaacatgt tctaccaaat gcgaaattgg
    12661 tgattgatcg attacttggt ttttccacgc ctttcaagga ggcgtaacct ttattcacga
    12721 gttggttgaa gctttccgag acaaaagacc cgacttgttt ttctccttat tgacaaagag
    12781 ccattaaaac tagagaaaaa attacaactg atcgctgatt atttgtagaa agaaaatcaa
    12841 agtattaccc aataaacttg agtcattagg caaaaaacac tgcgaaactc aagaatgatt
    12901 ttcgcagtgt ttttgtatat tttttattaa ctagtattac tacaaaggtt tatttttcac
    12961 ttctcaacgc agcacaaaat tgtctttact tatttactgg ttgggttcaa ccttctctac
    13021 tccgatacta atatttgttt gtatacccct tctaaaatcg gtagtttcca ttcaattgat
    13081 gggtactagt tgattttaaa aactatgctt attcaatttt ttattttagc tcatatattt
    13141 cgtttctttc taaattttat actgatgaaa acaagtagca cacccaatat tgacaatata
    13201 aactggctaa tgatggtttc tccagttttt ggaagatgct tatttccact gcttgttgga
    13261 ttgtttcctc ttatatctga gctatcagca acttttctat tctcttttgt taaaaagatc
    13321 gccacttctt gttctttttc gatcttgaaa gttactggtg tcgcatc
//
LOCUS       DAYYCQ010000078        13050 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000078 DAYYCQ010000000
VERSION     DAYYCQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.079"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1790)
                     /gene="uidA"
                     /locus_tag="ACHAYF_002250"
     CDS             complement(<1..1790)
                     /gene="uidA"
                     /locus_tag="ACHAYF_002250"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8579668.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIP"
     gene            complement(1813..3579)
                     /locus_tag="ACHAYF_002251"
     CDS             complement(1813..3579)
                     /locus_tag="ACHAYF_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8579669.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(3592..4386)
                     /locus_tag="ACHAYF_002252"
     CDS             complement(3592..4386)
                     /locus_tag="ACHAYF_002252"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8579670.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(4396..5772)
                     /locus_tag="ACHAYF_002253"
     CDS             complement(4396..5772)
                     /locus_tag="ACHAYF_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8579671.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(5892..6539)
                     /locus_tag="ACHAYF_002254"
     CDS             complement(5892..6539)
                     /locus_tag="ACHAYF_002254"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8579672.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(6623..8050)
                     /gene="uxaC"
                     /locus_tag="ACHAYF_002255"
     CDS             complement(6623..8050)
                     /gene="uxaC"
                     /locus_tag="ACHAYF_002255"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8579673.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(8124..9203)
                     /gene="uxuA"
                     /locus_tag="ACHAYF_002256"
     CDS             complement(8124..9203)
                     /gene="uxuA"
                     /locus_tag="ACHAYF_002256"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8579674.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9206..10837)
                     /locus_tag="ACHAYF_002257"
     CDS             complement(9206..10837)
                     /locus_tag="ACHAYF_002257"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8579675.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11141..11812
                     /locus_tag="ACHAYF_002258"
     CDS             11141..11812
                     /locus_tag="ACHAYF_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8579676.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(11972..12163)
                     /locus_tag="ACHAYF_002259"
                     /pseudo
     CDS             complement(11972..12163)
                     /locus_tag="ACHAYF_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(12531..12872)
                     /locus_tag="ACHAYF_002260"
     CDS             complement(12531..12872)
                     /locus_tag="ACHAYF_002260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579677.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT         4235 a         2566 c         1673 g         4576 t
ORIGIN      
        1 ttgggtattg atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct
       61 ctagtaaaga ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa
      121 tctgcaaaat tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga
      181 tttgcttcgt aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga
      241 aatccagcta ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt
      301 tccatccatc catctagctc ttttttcaac agatattttg catgtgccag atcccctccc
      361 gaaacatacc aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta
      421 caaacatctg gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca
      481 agttctttag ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa
      541 tctggttcat ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc
      601 aattccttga tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt
      661 ttttccacat tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt
      721 acttcatcaa taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa
      781 ggataatgtg atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta
      841 atatttacag cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat
      901 cctttaaaat aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg
      961 cgaataccaa attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt
     1021 ttcatactat ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc
     1081 attcctttct ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt
     1141 aagatttttc cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt
     1201 attcttgtat taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca
     1261 ggtctatgaa tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata
     1321 tccccatttt cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata
     1381 atattgttta cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca
     1441 gactcaaatg gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact
     1501 tttgcttcat gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta
     1561 ggaagagtca cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt
     1621 tcaaataaat cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt
     1681 ggttctaaag caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga
     1741 ccgcttaagt caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact
     1801 cctatctaaa tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac
     1861 tcccttaaat tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt
     1921 tccatcataa gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca
     1981 ctgtacagat acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc
     2041 tttacttgct ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat
     2101 gatcaagtca tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc
     2161 tatattttta attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta
     2221 tttttcaact tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg
     2281 tctatttcca gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa
     2341 aatcctttta tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt
     2401 aattgcttgg tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc
     2461 cagagcttct tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt
     2521 agcttttaac cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc
     2581 tttacgatag ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa
     2641 tagatcacat ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt
     2701 cattcctaac atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct
     2761 aagtaagtca gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag
     2821 atcaggatta aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg
     2881 taatccagct tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt
     2941 acctaaacta ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg
     3001 gaaatgttta gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact
     3061 aagttctaaa actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct
     3121 ccaatttcta ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc
     3181 ttcaatccca gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat
     3241 ttttacttct acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt
     3301 attagcagca attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata
     3361 tacctcatca gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat
     3421 attactcaag taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt
     3481 ttcttccaaa gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa
     3541 attatatggt ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt
     3601 agtaagattt ttccaaaaag tatctatata tttttgttca atatctacat aatctcccga
     3661 taaacattta aataaaacat cagttcttaa gttttcccat gattcttttt ctttattgac
     3721 caaaatagga taaaagaaaa ctttattttt atttgcagct tctagatcac caggagaatc
     3781 accaatcatt aatatcttat ccaaatcaca accattttca attacacttc gaataatatc
     3841 ttcttttttt cctttatctt gacaacacag ttcatcaact aatgataata attcatgtct
     3901 ttcccattct tcttgaacag cttccttatt agctgagctt acaataatta tcttcccata
     3961 ttttttcaga gcttttatag tatctctagc acttaaaaag ggtttatcta atccttttgc
     4021 ttgactaatc tgttgattga cttcttccga ccattttaaa gcttttaata aatcctctga
     4081 ctgagaatat tctatttccc tcttcaaaga tggattagac aattcttttg ttagagatac
     4141 ccagtgcttt aatcgattaa tcgatgttac atcatttttt tctaatgttt taactaaacc
     4201 taaaaatctg tttattccac gagtctctga atataaatta atattattcc aatcctgtag
     4261 gaatttttct ctatgatgaa catcaaaaat ctctgcagca attggtccga aaaagagatt
     4321 gtgtttataa gtcatcgtat ccataacaca gccatctgaa tcaatacaaa atatccaatt
     4381 attcatattt ctctcctatt tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt
     4441 atctttagat aatggataaa ggaaaaataa aattaatcct acaatcagaa aacaaattga
     4501 tggaattaat gttgaagcgt tatataactt ttctaaaaca tctcccgttt gtacatttgc
     4561 taaagagtca tagcctataa tagaaagagt ccaacctccg acgcctccag ctagagcttg
     4621 acctattttt ctagcaaaag aatataccgc atatacggtc ccatcttctc tttgatcaga
     4681 ctttacttct tgatcatcaa taacatcagt aatatttgcc caaataacag tattaaacac
     4741 acccaaaccc atatagccaa ccgaactaag aattaagaat acatacatat tactagcttt
     4801 tatcaaaaat aagatcaaat atactattgc tgaaaagaac attcctactg atgccagttc
     4861 tttttttcca tattttttac ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa
     4921 aataggattg ataaaattca ttaaaactat tccctgtgca tcattatagt aatcaggaaa
     4981 tacataatta ttcatactca taattaataa ttgagcaagg agtaagaaaa tagccgcacc
     5041 aattatagaa agtaatgccc tatttttaac taaccctttt aaagatttta acaatgaaac
     5101 tttgtattga gattggtcaa ttcttactcg ttcagtagtt agtttataac atattaaata
     5161 acaaataagt gcacatatcg aaaatattcc cgcaacaatt gtaaaagttg atccaccacg
     5221 aactatttga gcgcctgaat catctcttgt ataaataaat aatggtgtta atacaccaat
     5281 gaccaaactt gctaaagtag ctccaacatt tcggaatgtc gatagcattg ttctttcttc
     5341 tgtaatagaa gtaatagctg atgccattga tccataagga atatttatag atgtgtaaaa
     5401 aatactcccc cataatatat acgtaacata catgtatact accttaacgc tcatagaagc
     5461 tgaaatcata gaagtttgat acattaaaaa acttgctaaa gcgactggac cactcattct
     5521 taaaatccac ggtttaaact taccctgctt agttgctttc gatctatcaa caatacgccc
     5581 catagttaca tcagtaactg catcgataaa tcttgctact aaaaataatg ttccaacggc
     5641 agctccgctt atccccaata cttttgtata aaagatcatt aaaaaagaac tagcaaaaat
     5701 gaaagtaaaa tcgtttccaa aatctccaaa catatatcct attttgtctc ttataccaaa
     5761 ttttctttcc atacaattct ctcccttctg ttgttaagcg ttttcattct gatgaaagta
     5821 cgtactatac tctgaagcgt aaagagtcag tatttatttt ttacatttca gagtattatt
     5881 tgttttttct cctaataatt ctttatctca taaaacttat ctatatattt actagctact
     5941 cgacttactt cttcaaaatc acctttttca acagaagcca ataaattccc acctacacca
     6001 actgcaacaa ctcctacttc aaaccaatct tctaagttct ctatacttac tccgccagta
     6061 ggcattatat ttacgtgtgg taatggagct tgataggctt ttacaatgga tggaccaaaa
     6121 gcagaacctg ggaatagttt aatgatatct acgccagcag taagtgcctc ctttatctca
     6181 gtaattgtca tacaacctgg taaataagga atttgatata aattacaaat ctcagctgtt
     6241 tgtcgatcaa aagatggact aacgatatat tctgccccag ccataattgc gatacgagca
     6301 gtaaacgcat ctaaaaccgt accggctccg attattacat ctttttgatc agcatatttt
     6361 ttagcaattt ctttgattat ttcttcagca ttaggaactg taaaagttag ttcaatccct
     6421 ttaattccac ccgcaataat tgaatttatt gctttaatgg cctcatcctt atttttccca
     6481 cgtacaaccg caatgactcc agccgcttct aacttactta aaatttctac ttttttcata
     6541 tctatctcct tcctttatgg atacatactt tgtctagctg tactaactta caaagaataa
     6601 atttaagctt ttatttcaag tattattttc tacttccgat ttcaaaaaaa ttaaaataat
     6661 tatacgcatt acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg
     6721 gaattcggcc tgctttaacc cactcgctta gtaattgaca taatactcga cgaaaatact
     6781 catggcgagg atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca
     6841 acaaactttg ttgtgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga
     6901 accaccaagc acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg
     6961 ctgttgctag tggaagccaa tcagttggat ttaatgaata taaaattgtt tttggtaagc
     7021 acccactctt ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat
     7081 cccccatcga atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat
     7141 ttcttaaagc atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca
     7201 aatgtaattg aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc
     7261 ctgctaaagc ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat
     7321 aagtattcaa accgtgatct gctaatcgac agccaatttg atgaaaatga tccactcgtt
     7381 ggctcaatgc ttgcttcaac ccattaaagt ctttgattgg tatagccgct gtttgactaa
     7441 gttttaaaat atagttcgaa aagcctgatg atttgatact ccagacctta tctggacgga
     7501 aagtaggtaa taccttgaaa gaattatcct gtttagaaag ctcagtatga taccacaagt
     7561 catctgtagg atcatccgtt gtacagatgg ctttcacatc catcatctcc aacaattctt
     7621 tcgggcgata ggaatccgtt tgaatttttt cgttagcacg tcgccaaatt tctggtgcat
     7681 tcttctcaga aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt
     7741 caaaaatagg attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat
     7801 aatcatcacc tggaccactg atcaactctt cttctatacc attagctcgc atcaatcgcc
     7861 atttataatg atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt
     7921 gcttattttc tagcaaatat gctgggtcta aatgacaatg atagtcgata attggttggt
     7981 gttttgcaaa tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat
     8041 ctaaaaacat atttaaacac tcctttcctt ccgttagttt ttagaagaga aaatttttcc
     8101 tctaaataaa ctcccttata taattattta ctcgccccct tttcaagcgc ttcccaaata
     8161 ccatttaaat aaactgcacc tagagctcgg tcatataatc cataaccagg tctaccggtt
     8221 tctccccaaa tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac
     8281 attgttttta atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttcg
     8341 taaaaatcga gtccattgtc acttaaatat ttgatattac gaacatgtgc aaaaggaatc
     8401 cgatcccgtt gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca
     8461 atacttccag tacataatgt aataccatta gaaggagaat ccactacatt acacaaccaa
     8521 tctaaatcgt cacgattttt aacaatacga ggtaatccga aaatggaata tggtggatca
     8581 tctgggtgaa ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga
     8641 aaatatgcaa agttctctcg taatttctct tcatcaactt ctttataggc ttctaataat
     8701 tgcttaattt gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa
     8761 ttttctactt ctctaataat ttcctccgga tcagtaggga tattttttga aatataggct
     8821 aacgtagaag acccatcatc taaacgataa tccaaatccg attttaccca atcaaatact
     8881 ggcataaaat tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt
     8941 ttataattct caatgtattg atctcgtgtt ggtaaaccaa ttttgatatc atcatgaaca
     9001 ttaacactct caataacttt caaggtaagt ccattggctt cgacttcatc ttttaaagat
     9061 ttaattcgtt ctctcggcca aacctctccg gctggtatat ccattaatgt tccaacaatt
     9121 cctttcattg ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca
     9181 taccatctaa aagtcatttc catagttact ttcctccaac ttctgttata tatttattga
     9241 ttgttttacg aactgcttga ggacctacta gcatttcgca aaagattttt tcagttttct
     9301 ttcctacccc aacttgatat aaatctaaac caaagatttc tttattttct aaaatttcct
     9361 gtaccgcaca atgaatcttt gaaggatctt gttcacctaa tttcaatact gataatttat
     9421 cctgcaactc taccaataac ggatcaggac ttggcacaaa attttcccct tgatcatcga
     9481 ctgccagtaa ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg
     9541 catttccgtc tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt
     9601 gagaagtatc tgttgcaatt cgttgtggtg tatctggaat atagggatta ggcaaacgtt
     9661 tattaattac ttcatcaata aattgttttg gatcaataat gcctggattt tcaactactg
     9721 gtaaaccttc taaataacct attcctttga ttaaaccaac caaatcatca tcctgcatct
     9781 ctgctgaaat tgattgatat cctagcagac aacctgttac tgccaaagca gtatgaagtg
     9841 gattcaagct tgtcgtaacc ttcatgatgt ctgctttatc aaccgtctct ctatctgtca
     9901 taatcacacc agccttttcc aatttaggcc ggccatttgg aaaactatct tcaataacta
     9961 aataatgaac tacctcagta ttagtgaaag cagcaatatt ggtatgtttt tttgtgtgaa
    10021 tgatatctaa atcctcgagc cccatcatca atagatcttc ctttacattc tctgaaggat
    10081 ttggcgtaat tcgatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat
    10141 aagcaataaa tttttcagaa acatatcctt ctttttgcca agcttctgca atcgtaaaaa
    10201 tactatcttg aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg
    10261 gcaactctcc agcattaaat ctgctatata acaaggatgt tacaatactc atcgtatgtt
    10321 gaggattgct aggtccagat ttgatatcag ctttgactac cgaaaagaat tgccctttag
    10381 aatcttttaa actgtaacct ttttcagtaa tagtaaacgt tgtaaactgt aatttaggat
    10441 cttcaaaata tttaaccact ctttgatagt ccgctatatt cttcggattg caatataaac
    10501 tatcagccgt tactgccaaa agtctttttt tgagttcacc atctccactc ataacaattt
    10561 ccaaaatgtc attgttatag tcatgataag ctttttccac tacttcttca tcaaatgttt
    10621 cacacacaac tactcctgtt tctaattcat tttgatctgc taagcattga gcaatttctg
    10681 catgaaatcc tcgataaaga tttcctccac caaaatgaag ccatactgga tgttgctggc
    10741 cttcttgctt aacttctttg acatcaaaac acggcagtag aatctgcatt tcattgaatt
    10801 cttcgataga attttgccaa tttttaatcg aaaccataat taacctcctc aaactgatat
    10861 gttagttttg atacatttta tattttaaaa cccattgtaa ccgttgtcaa tataaaaagg
    10921 gaataaactt gcttttttct accatatcag aatgattgta ttttttattc agaaaaataa
    10981 aatactcctt ctttttggac tgattttatc gaaacttatt ttttcttttt ttcatgtatt
    11041 cttttatttt tataactagt gtattacttc aaaatttgat attatatggc atgaacagaa
    11101 ttaaaatatc agttaaaata aaagttaagg agcattgtgt atgacaatta aaactagtct
    11161 ccaaaatcaa gcttaccaat ctattcgccg acaaattatc tatgctgact tagaacctgg
    11221 aaaaaaagta tcagaaaaaa aattagaaga aacactttct attggtcgta caccaatacg
    11281 tgaagcactg attcaactcc gacaacaagg tcttgttgat acaattcctc aaagcggaaa
    11341 ttatatctca ttaattgatt tagattcggc caaaaatgct cgatttgtca gagaacattt
    11401 ggaacgacaa attatgttag agtgttgtgc caaaatgaat aaccaaaaaa aacggatttt
    11461 agaaactata atttcagaac aagaaaaagc tgttagtcaa aaaaatgctc gtagtttttt
    11521 tcataatgat aatttattcc ataaagcttg ttttgaaatt gcagagcgag aagaagtatg
    11581 gcaatggtta gaaaataaca atacacactt agaacgattt cgatggctac gagtaaaaac
    11641 ggaaggatta aagtgggaaa aaattatgga acaacattat caactatttg aggcattaac
    11701 aaaacaagat ccagaagaag ctaactttct aacatcgttg catcttcatt taatgcttaa
    11761 tgagcaaaat attgttttag ctactcatcc aaaatatttc aaaaatatat aacttttaac
    11821 tctttttaaa ttaaatggtt gttctgatca gaaaaatatc gcttgttttc ttattatgta
    11881 atatatttca taaactattc tgcataacaa ctattgtttg tactttttac attggataac
    11941 taattatgac tgctgataac ttttgatatt aaactagtat ctccaataca aataaaaaat
    12001 agatatcttg ttcatcaaaa ttagttcgtg gatacttacc ttcatttaca tcattcagcg
    12061 gtttcccatt ttcttttatt ctattatttc attttctaat agacttggct tcatttcgca
    12121 atactttttt tcaataggca tcaaattggt gttgtttaga tatttagttg tagttttgtg
    12181 acatgataag tatgctcctt tttctctttt ccactaatac aacgatcaaa gggtgctttt
    12241 ttcaaaaaac tagaaaaatc aaaataagat tcgtattttg attctatata tcctgtttca
    12301 actacatctt atatttcaca atagaataaa tagccttttg catagctgct taacttataa
    12361 gaaattgcaa tttacttctt caaagtgaaa acgaatatga gtaaaataaa attattcttc
    12421 ttacagataa ttaaaaatag cgcgtaacaa aaatcggcat tcaatcacat aattaggatt
    12481 gatgccgatt acttatattc tacgtatagt tttagtattc ttatttttta tcaccagtaa
    12541 ccaattgaga acagccctaa agcagaatag tcttttatct caaaacgctt aatttcaggc
    12601 ttaggaatat gaataatctt ttgagtatat tctttctctt ttaatcttac tgaagaaata
    12661 ccactgccta tcttttttac aaatatttca tttggtgtac agactaataa aacaatattt
    12721 gtaatcaatc cttgcactaa agaattaaaa ggaagactat ttaaaacagg gtgtgcataa
    12781 ctataacatg tgaagtagtt attttgaact ttaactttta aaaactttaa aaataatctt
    12841 atttgttctt ctaaacaatc atctatactc atataatcac tccactaatt aatctcccca
    12901 aaaagacaca gcattaagag atataccaac acaacagtgc aaataactct acatcttcct
    12961 tcgcaacctt atttcaataa accataaaaa ttatttttta atagaaactg aaaattaaaa
    13021 ctagaaaaag tagttttacc gaatgtaaga
//
LOCUS       DAYYCQ010000079        12846 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000079 DAYYCQ010000000
VERSION     DAYYCQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.080"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHAYF_002261"
     CDS             6..305
                     /locus_tag="ACHAYF_002261"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8579678.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHAYF_002262"
     CDS             378..1739
                     /locus_tag="ACHAYF_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8579679.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHAYF_002263"
     CDS             1761..2021
                     /locus_tag="ACHAYF_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579680.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHAYF_002264"
     CDS             2035..2787
                     /locus_tag="ACHAYF_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8579681.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHAYF_002265"
     CDS             complement(2881..3486)
                     /locus_tag="ACHAYF_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8579682.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHAYF_002266"
     CDS             complement(3539..4513)
                     /locus_tag="ACHAYF_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8579683.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHAYF_002267"
     CDS             complement(4891..5610)
                     /locus_tag="ACHAYF_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8579684.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAYF_002268"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHAYF_002268"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8579685.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHAYF_002269"
     CDS             7161..8558
                     /locus_tag="ACHAYF_002269"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8579686.1"
                     /translation="MKTYTFPEKFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHAYF_002270"
     CDS             8639..9079
                     /locus_tag="ACHAYF_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8579687.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHAYF_002271"
     CDS             9227..9553
                     /locus_tag="ACHAYF_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8579688.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHAYF_002272"
     CDS             9554..10303
                     /locus_tag="ACHAYF_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8579689.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHAYF_002273"
     CDS             complement(10383..10838)
                     /locus_tag="ACHAYF_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8579690.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHAYF_002274"
     CDS             complement(11265..11540)
                     /locus_tag="ACHAYF_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579691.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACHAYF_002275"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACHAYF_002275"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8579692.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4110 a         2214 c         2623 g         3899 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaaattt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtt
//
LOCUS       DAYYCQ010000080        12732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000080 DAYYCQ010000000
VERSION     DAYYCQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.081"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHAYF_002276"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHAYF_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8579693.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHAYF_002277"
     CDS             complement(322..891)
                     /locus_tag="ACHAYF_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8579694.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHAYF_002278"
     CDS             1077..1979
                     /locus_tag="ACHAYF_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8579695.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHAYF_002279"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHAYF_002279"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8579696.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHAYF_002280"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHAYF_002280"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8579697.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHAYF_002281"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHAYF_002281"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8579698.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHAYF_002282"
     CDS             8547..8732
                     /locus_tag="ACHAYF_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8579699.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHAYF_002283"
     CDS             complement(8794..9990)
                     /locus_tag="ACHAYF_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8579700.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACHAYF_002284"
     CDS             complement(10160..11596)
                     /locus_tag="ACHAYF_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8579701.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACHAYF_002285"
     CDS             11889..12698
                     /locus_tag="ACHAYF_002285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8579702.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4009 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt ga
//
LOCUS       DAYYCQ010000081        12446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000081 DAYYCQ010000000
VERSION     DAYYCQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.082"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      complement(22..88)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(130..777)
                     /locus_tag="ACHAYF_002286"
     CDS             complement(130..777)
                     /locus_tag="ACHAYF_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8579703.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1056..1559)
                     /locus_tag="ACHAYF_002287"
     CDS             complement(1056..1559)
                     /locus_tag="ACHAYF_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8579704.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1682..2446)
                     /locus_tag="ACHAYF_002288"
     CDS             complement(1682..2446)
                     /locus_tag="ACHAYF_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8579705.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2553..2828
                     /locus_tag="ACHAYF_002289"
     CDS             2553..2828
                     /locus_tag="ACHAYF_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8579706.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3505..4455
                     /gene="yidC"
                     /locus_tag="ACHAYF_002290"
     CDS             3505..4455
                     /gene="yidC"
                     /locus_tag="ACHAYF_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8579707.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="ACHAYF_002291"
     CDS             complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="ACHAYF_002291"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8579708.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5605..6063)
                     /locus_tag="ACHAYF_002292"
     CDS             complement(5605..6063)
                     /locus_tag="ACHAYF_002292"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8579709.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6060..7523)
                     /locus_tag="ACHAYF_002293"
     CDS             complement(6060..7523)
                     /locus_tag="ACHAYF_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8579710.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="ACHAYF_002294"
     CDS             complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="ACHAYF_002294"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8579711.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8598..9347
                     /locus_tag="ACHAYF_002295"
     CDS             8598..9347
                     /locus_tag="ACHAYF_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8579712.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
     gene            9614..11092
                     /locus_tag="ACHAYF_002296"
     CDS             9614..11092
                     /locus_tag="ACHAYF_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8579713.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(11160..12251)
                     /locus_tag="ACHAYF_002297"
     CDS             complement(11160..12251)
                     /locus_tag="ACHAYF_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8579714.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         3877 a         2526 c         2103 g         3940 t
ORIGIN      
        1 aaagaaaggg gctatcccca gactttagta ggcgtcatta gctatctttc gttagcattt
       61 aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa
      121 acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt
      181 cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata
      241 cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gacacattcg atgttttctg
      301 acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa
      361 tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat
      421 gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt ttttcacttt
      481 tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgga cgatttgctt
      541 cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt
      601 taaaaatgta tcgatttctg gcagttcgtg ttctaatatg tctcgaatct tttcagctgc
      661 tagtgcctga actgtttgta ccgtttttgg atcattgaca gatgagtacg catttaataa
      721 ctgatctact tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat
      781 tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa
      841 agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg catcttatca
      901 tcaccccctt taatgttcat tcttctatgt acatgattca ttcgataaat aaaagtaaac
      961 cgtatcttga acagatagtc aactgcaaac agaaaaaacc gagaaaatca gccagatttt
     1021 ctcggaatat agtctattat tattggtttt acctattaaa tcgaggattc tttcccaaag
     1081 taacgttgcc atctcttacg catacttgca gacatgggct gagccgcttc tttgactgcg
     1141 cagtatttgt ccacgaatgt cacaatatat ccttctttat acttaggcgg tgcgatcgtt
     1201 gcaccccaca tgtgcttcaa aatgatatct cgctcaagat ccgacaaatc ggtgattttt
     1261 tctgcattct ttacagcaat tcttggatgg atatacgcat gtgtcccctc gtcaaatttc
     1321 gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta
     1381 gcattgccac cccatttttt tgcgagcaga taactatagt aagaaacact gatggaatgc
     1441 tccaaacgag tggaatgaac atgctgtgtg taattcgcta attttttcac tgcttctgtt
     1501 tctaaaagat cttgtacata agacatatat tcttcgtctt cacgccattt ttctgtcata
     1561 attatcgatc tgccaccttt ttgaatttca ttttcttctg aaattgatct aagtatacca
     1621 ccagtgttcg gattattcta gtcgctctag ataaaatagc taaagcttaa tagttcctta
     1681 atcataaaga aaaatgataa agaacgatcc ctgctgcgac tccgacattc aatgattccg
     1741 catttccttt cattgggata tatacgttct gatcagtcaa agacaagatt tcctgactta
     1801 ctccctgacc ttcgtttccc atgattatag cgaaatcctc tgttttttct actttgtcta
     1861 atgcatgtgc ttcttcattt aattctgtac cgtacaccgt aaaccctttg cctttcatta
     1921 cagggattac atctgccaaa tctttttgta acacaggcaa atgataattg cttccctgca
     1981 tgcttcgcag cacctttgta ctataaatat ctgccgttcc tttacctaat aaaacaccag
     2041 ccataccaaa cgcatctgcc gtacggatca tcgttcccac atttccagga tcctgtacat
     2101 tatctagtaa aagccagcct cctgaaaaat ctggcgtatc ttcagatgtt aacgtcatca
     2161 ctgctaagat cccttgcggt gtaggtagat ctgacatagc ggtcatgacc tcatctgaaa
     2221 ctagaaaagt agaaatctct ttgtttacag ccagccagtc tccccattct ttcatacctc
     2281 tctgtgtcac aaaaagttct tttatttctg cattagacga cgcagcttct tcgatcagat
     2341 ggaacccttc caaaagatat tctgcctttt cttctctgta tttcttttta tgtagttttt
     2401 ttaattcttt gattcttgga ttttttgctg attggatctc tttcacattc ctcacctcaa
     2461 ctgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt
     2521 gatttataaa acaggaaaga aaggtgacga acatgcgcaa agttaaaatg aatgtccaag
     2581 gacgtgtcca gggcgtggga tttcgctata tgaccaagat ggttgctgat cagttaggcg
     2641 tgaccggaac ggtaaaaaac gaagacgacg gctcagtcac aatcgaagca atcggaaatg
     2701 acgacatcat ccaaaaattt atcgaagaag tcaagaagtc tccgagccca agtggacgag
     2761 tgcagtatgt ggatgtccaa gaagatccga tgattgagga acgaaagaaa tttgatgtag
     2821 tgggataagg ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt ttgtgaaaat
     2881 ggctttccac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     2941 tcgataaggt tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca ttttcaaaat
     3001 agcttctcat attttgaaat gtaatgactt aataccgaga ggcagctgat aaaacaccgt
     3061 caataaggtt gtgaaaaagc tggtctgcat caagcattaa ggactggttg tgataaatag
     3121 ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt tgaacaccgt
     3181 cgataaggtt gtttttgagg cgctttgcct caagtattaa agacaggtag taaaaaagct
     3241 gatctataac ttgcaatgaa tttgcttaac ccatattttc cagacagaac aaaaaagttt
     3301 ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcta agtatttctt
     3361 tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaacgaa tatttatatg
     3421 aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa
     3481 aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga
     3541 cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct
     3601 gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat
     3661 ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac tgtgatcgtt
     3721 cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat ccaatctgaa
     3781 aaaatgcaag cgttaaaacc acaagttgaa gcagcacagc aaaagctaaa agctgcaaca
     3841 acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg cgaaaatgga
     3901 ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct
     3961 gccttgtatt ttacggcacg ctacacagaa ggtatccggg aatcatcttt ttacggcatc
     4021 gatctaggct caccaagtat ggtgcttgtg gccatcgcag gggtcgctta tctgcttcaa
     4081 ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgctg
     4141 atcgtctctc ctgctatgat cgtcttcatg tcaatcagtg cgccagccgg tgttacactt
     4201 tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg
     4261 aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc
     4321 gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa acaagcaaca
     4381 aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc aggaaagcag
     4441 cagaagcgga agtagatagt aaataagagt gtaataaaaa ttcattaaca gttttagcaa
     4501 ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt cgatattttt atttaagtga
     4561 atgtttaatt tgaatcatcg attgatcgct atctaattgc agcagcatct ttttatgaag
     4621 aaacaacttt ttcttatcta tcaacgtaga caggtgttgc tgtttcttct aatagtgtac
     4681 ttaattcttt aatattttgt tttccttctt ctaacatcca actactcgct ttttcttcgc
     4741 cttcggtaac aaaaggtgcc actcccttcg cccatgttgc tcgcccacat aagacaccat
     4801 tgaatgtcga acctgattgt tttgcaaaac gtaatgtatc ttgaaatagc gtagcattga
     4861 ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca tagaaatacg
     4921 cggatgcctc tgcttgacta taacaagtct cttgcccata tccttccaca aatctcatat
     4981 tcactggcac ttctactttc aatacatcca cttgataacg ttcttttgaa aattctcgca
     5041 tggcttcaat aactttatgt ggttttactt ttgcataggc aagagaagac ggatcgccaa
     5101 tagttgcatc ataagaaaga atctctaaaa agaaaggtat gccttctgct tcgcattctg
     5161 aaccaatccg ttctataact atttgttttt gcacattaat ctctgctggt tcatcaacgt
     5221 catagtacaa taaaaattta caagcatccg caccagcttc ttttaacctt ttcaccgacc
     5281 aaatattcaa taaatcaggt aatcttcccg gtattttcgt atcatagccg gttttttcat
     5341 aagctagcag taagccacat tctttctctc gaagcgctgc tgcaggcagg ccgtattctg
     5401 gatcaagtaa aatagctgag gcatagggag tcaggtattg agaaaccatc tgtttaaatt
     5461 ggatgatgtc ttctgatgta gcttctttat attggttgat cattttcttc aaagcaccac
     5521 gctggtcgat tgctaatgcg ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt
     5581 cctgtttttc tatactcatc ttcttcatag atcttcacct atttctttag ttaatattgt
     5641 ggctatttct gtttttgatt ttgcttttct caactgttct gtaacctctt ttcgcatcaa
     5701 tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg aatctggaat
     5761 aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt caattccttt
     5821 gttcagtgtg gcgataatca tactaggttc gttgattgtt tgagacttcg catgagggat
     5881 agcaaaacct gacattattc ctgtagtccc ttctttttct ctttctagaa gtttttgata
     5941 tacttctttt tcgtcaagag cgatccctaa acagaatgtt tgctttgcta aaaacttaaa
     6001 tgcttcttct tgactatcca atggctgttg taaaaaaatg ttctcttcat gaatgattgt
     6061 catgataagt ctcctgctct tcctttttcg ataactgtac gatttgctgc tttattatcc
     6121 atcttcttgt ctttatcagt tactcttttc ttttggatta atcctagtat cactccacca
     6181 acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac tacaggtaac
     6241 actaaaaaac caccatgtgg tgctggaact tggacgccaa acaaataagt taagacagca
     6301 gcaattgatg agcctaacat catcgtgggt aacactcgga tcgggtcttt cgcagcaaac
     6361 ggaatagctc cttccgtaat atgagttgat cctaaaataa aattaaccaa acctgcatta
     6421 cgatcattgg tatcaaaata tttaccaaac aataaagtag caaaaccagt aatcaacggt
     6481 ggtgcaatac aagctgctga cacaccagcc ataaaagtcg tgtttccttg tgccaaaaga
     6541 gctgttcctg ttacatatgc cgccttatta accggtcctc ccatatcaaa cgcacacata
     6601 cagccaacaa tcacgccaag aacaagtgga ttcgaatgtt caaatccagc taaaaagctc
     6661 atcattcctt catttatagc cttcattgga cctgccagca aagacatcac gatgccaata
     6721 aatgctacac caataactgg atataagaag attgccttca agccatctag ttgtttaggt
     6781 aaaattgtca gtagcttttg caaaaataag ataaaataac ctgctaagaa cccagctaaa
     6841 atacctccta aaaatccagt accgccattc atggctacga ttccaccaac aaatccaacg
     6901 actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac tggaaccatc
     6961 attcccattg ctgcatcgcc aactgctttt accattgccg ctgtagcatt gtattgtgca
     7021 tgatctggat caaaagaata gattccccaa acggcaaatg aaatagcaat caatactccg
     7081 ccagctacca cgaaaggcaa catatgagag acaccgttca ttaaattttt atagatgcta
     7141 cgaccaaaat tagcaccact catcgcttct ggttctaaag tatcagcagc ttcattatct
     7201 gctaagacag taccttttcc tgccaatgct tcgtcaatca gccgttgtgc atccttgatt
     7261 cctgtactga cagaaacatc aatgactcgt tttcccgcaa atctttctga atggacatct
     7321 ttatctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt taattcattt
     7381 tcgatcccaa tttgtccatg ggtctccact ttgatcttga tgcctttttt cctagctgct
     7441 tgttctaatg cctcttgcgc catgtaagta tgcgcaattc ctgtcggaca accggttgct
     7501 gcaattagtt gatattttcc catgtttcaa acctctcttc cttgattttg atttgtttac
     7561 ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcggtt gagctgcctg
     7621 ctgctatgct tttacgtaac gtttcatcaa gcggtcgtcg attcatatat cctgctaaaa
     7681 aagcgcctaa catggtatca cctgcacatg ctgtattgac aacttttcct tttggcgagt
     7741 ttcctcgaaa caccctgtct ttcgtaaaaa gaatcgctcc tttctcaccg agggataaca
     7801 aaacattttc tgcacctaac tctactagtc gttgtccata aacaaagtaa tcttcatctg
     7861 taaccatttt tcgtccgaac caagaagaaa gttcttcctc gttcggtttc agcaaaaacg
     7921 gtcgataggg taaacaatcc atggcttctt gatcactact atcaataatc aaatgaatcc
     7981 ccttttcctg acaaatccga ctgatttcta ttaaaacctt tggagacagc cctcttggta
     8041 aactcccaga aacacaaaga taatcttctg cttgtaatgc tcttatttga tttaaaaaat
     8101 tatggacagc tgtctgcggt attttaggtc cttgattcac taatttgtat tcttctcccg
     8161 tttgattgac ttgtgtaaat acattgatac gcgtcatacc aggcacttca ataaactctg
     8221 tttggatttg tttctgctgt aaatagtctt ctatatactt tcctgtaaac ccagcagaaa
     8281 aacctaatgc cgtacttggg aaatctagca tttttaatac tagtgacaca ttcacccctt
     8341 ttccgtttgc ctgaatgtca tcctcttttg tgcggtttac cataaaagga tgcatctcct
     8401 ctgtttcaat aaatagatca attgccaaat tcatcgtgca tgtgtagata gacataacca
     8461 acgacctcct tgactctatt atgaaatacg cgaaagagaa aatgttttct taatcgtccc
     8521 aacacaaaga aaacctttac atttataaac tcgtcgtgct attttttaac taacttttgg
     8581 tgaaagaagg aaagaatatg gatcacaaat taagtgaaac agaacagtac ctctggaact
     8641 ttatcgaaca ccacatccta gagatcccta actactccat cgtgaaacta agtgaacaag
     8701 caaatgtctc aacagccacc attgtgcgga caatgaaaaa gaaaggctat gaaggattta
     8761 cttcgtttaa gcaccatcta aaagaaggag aaaacaagaa catcaatttt tctttcttag
     8821 ataaagtaga caaaggtatc cgtcgagcca ttctaaaaaa tgaacaagaa gtggtacgta
     8881 cgatcaatat gttggaaatc ggaaatattg aggatgcaat acaaagaatc aaatttgctg
     8941 accgggtttt tgtctttgca cgaggatttt cagaaatgat tggtcaagag atgctcgtaa
     9001 aacttcaatt aacagggaaa aattgtcaaa tgcatactga tccagaaatc atcaaaagca
     9061 tcagtcggaa actaactaag aaagacagcg ttatttttgt ttctttgaac ggcgagacaa
     9121 aagaactcgt caccgccgca aagaattgct atgatgcaga gattggttcg attctagtta
     9181 cagccaatca ttcctctaca ctgaaagact attgcgagat caatctcgta ggtttcaaat
     9241 cagaaggttc ttatttccct gattatgaag tacggtcacg tttgcctctc cagattatag
     9301 cgagaatcct gcttgacgcc tatgtattgc gtatggggga gaattgaaaa gcagtttatt
     9361 atggttggtt acagtttaaa acatcacatt ttttattctt tatattgtat ggactcacgg
     9421 aaacttttta tgtataaagt tattcacaat ctataaagtt actttaaata acaaaaaaat
     9481 aattgaaaat acttaaaatc aaaaaatatc tttttaatca ataaaaaaat tgacagtttt
     9541 attaataata agctattctg ttatagaaga aaaaattgtt tgttattatt agcaacaaaa
     9601 aaaggagtgc atcatgaaaa agttatctca aaaacgttta gctcaaatca ttattgattc
     9661 tagaaaatct ctgaaaatga cacaacaaca acttgcagta gctactggta tcaaccgtgc
     9721 cttaattagt cgattagaat ctctcgattt tatcccgtca atacaacaat tagaatcatt
     9781 agcagaagtt ctaaattttg aaccaacaga tctatttata aatgaatcaa ataaatcaaa
     9841 cagaacaaaa catcagtcga agaatattgc tgtagccggt actggttacg taggtctttc
     9901 cattgctact cttcttgccc agcacaatca ggtaacagca gtagacattc ttcctgaaaa
     9961 agtaaaacaa attaacaata agaaatctcc aatacaagac gattacattg aaaaatatct
    10021 agctgaaaaa aatcttaatt taattgcaac gactgatggt gcagccgcct atagagaagc
    10081 cgattttgtt gtcattgctg ctccaacaaa ttatgatagt aaacaaaatt attttgatac
    10141 ttctgctgta gagtcagtaa tcaaattggt gttagaagtc aatccaaagg caataatggt
    10201 catcaagtcg acggtacccg taggctatac cgaaagtatt cgtaaaaaat acaatactaa
    10261 aaatattcta ttcagcccag aatttctcag agaatctaag gcattatatg acaatctata
    10321 tccttcgcga attattgtcg gcacggacca aactgacaaa actttacgag aagcctctca
    10381 cacttttgca gatttactcc aagaaggagc aatcaaaaaa gatatcgaaa ctcttttcat
    10441 gggattcaca gaagctgaag cagttaaatt attcgctaat acttaccttg cacttcgagt
    10501 atcttacttc aatgagctgg atacttatgc agaaatgaaa ggactaaata cacaagaaat
    10561 cattcacggg gtttgcttag atccaagaat tggcacacat tataataatc catcttttgg
    10621 atatggaggc tattgtttac cgaaagatac aaaacaactt ctcactaact ataacgatgt
    10681 tcctcagaat atgataagtg caattgtcga tagtaatcgt acaagaaaag actttatagc
    10741 agatcgagtg ttagaacttg ctggcacgta tgaggctaac agttcgtgga atgcagaaaa
    10801 agaaaaagag gtagtcatag gtgtataccg tcttacaatg aagagtaatt cagacaactt
    10861 ccgtcagtca tctattcaag gagttatgaa gcgaattaaa gcaaaggggg caactattgt
    10921 aatttatgaa ccaacacttg aaaataacac tacatttttt ggttcaaaag taataaataa
    10981 tttagatgat tttaaaaata tcagtcacag tattattgct aatcgttacg atagtaatct
    11041 tgatgatgta aaagaaaaag tatataccag agatttattt caacgagatt aaaaaataac
    11101 acctcagatt tgtagtgtct gaggtgtttc tataaacttt tataagtaaa aaaatacaat
    11161 tatacttcat aaaaagactt ataccactgc gcaaatttac gaagcccgtc acgcaaactt
    11221 gtacttggtt taaattcaaa atctcgttct aacgcactag tatctgcata agtaactggc
    11281 acatctcctg gttgcattgg aactagttcg gtatgagatt caaagtcgta atcttctggt
    11341 agcacccctg ctgcaattag ctcttgttgt agaatatcta caaaatctag taggttttcc
    11401 ggcgtattgt taccaatatt ataaatatga tatggtggga ttggtagccc atcttcccca
    11461 tcaattttct ctggagcgca ctgcataacc ctctttactc cttcaacaat atcatctata
    11521 taggtaaagt cacgcttgca attcccataa ttgaatattt ggatattatc accagcacga
    11581 agtttatcag taaaaccaaa ataagccata tctggacgcc cagcaggacc atatactgta
    11641 aagaaacgta accctgtaga cggaatattg taaagtttag aatatgcatg tgccattgat
    11701 tcattacttt tcttagttgc tgcatacaac gatacttgat tgtctacttt atcctctgta
    11761 gaataaggaa ttttcttatt agtaccataa acggaagacg atgaggcata cactaaatgt
    11821 tccacaccag attctccatt atcatatgaa tgacgacaag cttcaagaat attatagaat
    11881 cctattatgt tagattcgat atacatatct ggattagtaa ttgaataacg gactcctgcc
    11941 tgagctgcta aattcaccac gatacttggt ttataattat caaagatgaa atcaattgtt
    12001 gctttatcag caatagaacc tttgataaaa ttccaagtag atcctggatg gttctttgcc
    12061 tccgtttcta tctctgataa tctatagttc ttaatagaga catcatagta atcattcata
    12121 ttgtcaatgc caatgatttt tattgatgac tgagtacgta ataactcaag aaccaaattt
    12181 gatccaataa aaccggctgc gccagttatt aatattgttc tatttgataa ggacgtgttt
    12241 aattccagca ttttttagca ctcactttct ttttttctta tgaatacata tttgttatat
    12301 attaaaaaat cttatttaga cttatctatt gtcactttta tcattctaat ctttgcacgt
    12361 agtagaataa ttatacagat aggaaattgc taagcaaccc acttctttct attatctatt
    12421 aacttattct aaaacgaata gaaatt
//
LOCUS       DAYYCQ010000082        12170 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000082 DAYYCQ010000000
VERSION     DAYYCQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.083"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACHAYF_002298"
     CDS             <1..276
                     /locus_tag="ACHAYF_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8579715.1"
                     /translation="PSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKF
                     YRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(892..1932)
                     /locus_tag="ACHAYF_002299"
     CDS             complement(892..1932)
                     /locus_tag="ACHAYF_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8579716.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2281..2607)
                     /locus_tag="ACHAYF_002300"
     CDS             complement(2281..2607)
                     /locus_tag="ACHAYF_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579717.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2594..3382)
                     /locus_tag="ACHAYF_002301"
     CDS             complement(2594..3382)
                     /locus_tag="ACHAYF_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8579718.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3750..4040)
                     /locus_tag="ACHAYF_002302"
     CDS             complement(3750..4040)
                     /locus_tag="ACHAYF_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8579719.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4176..4355)
                     /locus_tag="ACHAYF_002303"
     CDS             complement(4176..4355)
                     /locus_tag="ACHAYF_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579720.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4352..4702)
                     /locus_tag="ACHAYF_002304"
     CDS             complement(4352..4702)
                     /locus_tag="ACHAYF_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579721.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4695..6020)
                     /locus_tag="ACHAYF_002305"
     CDS             complement(4695..6020)
                     /locus_tag="ACHAYF_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8579722.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6470..7084
                     /locus_tag="ACHAYF_002306"
     CDS             6470..7084
                     /locus_tag="ACHAYF_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8579723.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7249..7491)
                     /locus_tag="ACHAYF_002307"
                     /pseudo
     CDS             complement(7249..7491)
                     /locus_tag="ACHAYF_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7695..9014)
                     /locus_tag="ACHAYF_002308"
     CDS             complement(7695..9014)
                     /locus_tag="ACHAYF_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8579724.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9148..9756)
                     /locus_tag="ACHAYF_002309"
     CDS             complement(9148..9756)
                     /locus_tag="ACHAYF_002309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579725.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10394..10651)
                     /locus_tag="ACHAYF_002310"
     CDS             complement(10394..10651)
                     /locus_tag="ACHAYF_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8579726.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10644..10913)
                     /locus_tag="ACHAYF_002311"
     CDS             complement(10644..10913)
                     /locus_tag="ACHAYF_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8579727.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11044..11223)
                     /locus_tag="ACHAYF_002312"
     CDS             complement(11044..11223)
                     /locus_tag="ACHAYF_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579728.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11321..11557
                     /locus_tag="ACHAYF_002313"
     CDS             11321..11557
                     /locus_tag="ACHAYF_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579729.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            complement(11604..>12170)
                     /locus_tag="ACHAYF_002314"
     CDS             complement(11604..>12170)
                     /locus_tag="ACHAYF_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579730.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3721 a         2228 c         1849 g         4372 t
ORIGIN      
        1 ccatcaattg ctagtgcctt taagaaacta aaagaatacg gattttatca agggacagaa
       61 catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga
      121 cgcaataaat tctatcgaag tttacgcact gcctcaccca cgattaaagg catggaagcc
      181 atccgaggat tatataagaa aacccgaaaa gaaggcactc tcttcgggtt ttcggtctgt
      241 actgaaatca aggtattatt gggaatccca gattaaatca tagataccgt aagggatttt
      301 attctttatt taaaactttg caacagaacc gtccgttgcg ggtaataccg taacactgaa
      361 gggcacgcat taaggcgtca tcttaatgct ttcctgtagg acactgacgg agattaccag
      421 tctcccgagc cttacagggg cctttctttt ttttgtattt tcaattatca caatttatcg
      481 tccaaaagtg cagaacgaaa atcaattcat cacaagcata tcagacaatt aaatttaaaa
      541 caactaaaaa tatacaaaaa agtgtaattg aatattagca gcactgcctg aaaagaagta
      601 atatcaggct tttcagcttg gggaaataag tgaattacct ctcttcatga attattataa
      661 ctcaaaaaag cacatatgca aacactagtt ccctattttt ttgaatgaaa aataactaaa
      721 atatctaacg attatgactt taaaacctac gtttgtgatt gttttcataa acttttaatc
      781 taaaaactcc ggaataatag ctatttattt gtaacatttg taataaaatt aaaatcataa
      841 aaaagttcta atttaaaatt gatcattttt atagtgatcg ctttttatca actactctag
      901 ccaattttcc gtactaactg ttggtaattc gttaaatgtt tctctttgtt cgtttgcaag
      961 ctttgccgca atagcttttt cgacaaacca attttttaca taaacgaaaa ttagattctg
     1021 caaattgttt actttttcag ttttttgttt ttgataagct ttcagcaaag tcatatataa
     1081 tcctttgtca gcatcaattc cataagattc cagttcttca taaacaacaa ctgtttgctc
     1141 taattcttca gcttttttct ttgcattatg aatagtttgg acggtttttt gagcgtcttc
     1201 gtaagtacta gaaaatgttt ttatcaagtt taatacattt tctggtacaa atgtatttat
     1261 actgtcctct ttcatgatgt cgacgaagtt atctagtaaa acttgatttt gtaactgatc
     1321 agtttcagtg tctattatgt gtctattagt gtctatagaa ttagaataga atagattgtc
     1381 tgcgattttc gcaattacgg ttgagtcctt gatatgactg gattttgccg tatttgcgat
     1441 tttcgcaatt acggtggtat cattgatttc tcgcggtttt gccgtatttg cgattttcgc
     1501 aattacggtg gtatcagtat tgtcttcctc ttccatgatt ttaaagacgt cttgattcgt
     1561 tacttctggc ttgcctaagt acaataaatt aggcatggat tcaatcttac cgtcacgttt
     1621 atttactctt cctctcttct gtttcagaag acctgctttt tctaattcct ttttgatttt
     1681 agatacttta ccttcgcggc attgtaacaa tttcattaat tcttcaactg tgaatatgaa
     1741 aaatatatta tcatcagaat ctacccagtt atttttgact gaataatcaa atcgatcttt
     1801 aagtagcata taagcaattt ttgcatcatt tgatagatcc atgtattttt cgttagtaaa
     1861 aaatacctta ggcatttgat aatatttttc acgataaatt tcttttgatt tgtaataatt
     1921 aaaatcattc atagccaatc ctctttctgt aggatagcgt tttgttcaaa actatgttac
     1981 aatgaattaa acaaatatat ctatccacta tatatttttc tactgctgat aagtcgccaa
     2041 actttgttaa agcagtagtc tttttttatc tttttttagt ttaccttaaa aaataaatta
     2101 aagcaaaccg ctctatttca aggtttaagc tcatagtaac tatatcacga tattacaata
     2161 atattatatc acgatatcgt aatattacaa tatgaagata agcgacttat cagctttgat
     2221 actactgatt tttataaaaa aaatatcacg atattatgat atcgtgatat tgaatgtaaa
     2281 ttaaatttta ttttgtttct ttgctagttt atatccttca atcaagtatt cttcaggtat
     2341 ggactttaat agcgcatctt ctataacgtc tggtttactt ttgtctgatt cagcaatagt
     2401 cgtccaatat ttgagtgctg acattagaat tggatttata cgaatgctat ctgctgttct
     2461 tacactactt ccttgatctt ttatgacttt acttatattt ttcttggtta attcgatttc
     2521 ttctacttct tttgatttaa aattcttttt atttttattt cctagtaatc tacttactgc
     2581 agcttgactg cgattatctt ctgccatcaa aatcaccaac ttcttttaaa tattcttctg
     2641 taaatgcttt atagtcatct tcagctgttg agggtttatt aaaattgatt agatctgatc
     2701 cagcagcttg cgcttcgtca acggttattg tcctacgaat ataagttttg aaaatttttg
     2761 caccaatgac tctagaaatt tcggttgctg cagacgtcat ttctttagaa atgttataac
     2821 gcttattata cttagtaaat agaactcctc gtacttcaag cttgtcgttc gtatattctt
     2881 ttaccgtttc aatagtttca cttaacttta ttagtccttt taatgagaaa tacgcagctt
     2941 caactggaat gataatttca tcagaaacag taaatgcgtt gactgtaagc attcctaaac
     3001 ttggtggaca atcaattata atataatcat agttgtcctt gattggttga agtactcttt
     3061 taagcctgtg ttcacttcct acttgagtaa attccctctc tgcagaagaa agggctatgt
     3121 cggctggcaa aacatcaatt cctttatagt ccagaatagc atctgtaatg tcagcctccc
     3181 cttttaatac ttcataaatt gtaggttggt ttgttccatc tatatcgaaa cctacattat
     3241 ctgtagcatt tccctgagga tcaatgtcca ctaaaagtac atttttaccg taaacaaatt
     3301 tcaaacatgc tgctaaatta acgctagtcg tagttttaga aactccacct ttttgattgg
     3361 ctactgacaa tattcttgcc attaaaatat cccccattca tcttatatta taatataatt
     3421 atatcataat attgtgatat tgcaatatcg tatatgcatt atattgtaat attgtgtgat
     3481 tattttgttt taaagatata tatcggttcg gatataaaga ttattcatat gttattcgaa
     3541 attaaaataa tcacttttta ttactttaaa attttttatt aatgataaga aatatgtttt
     3601 gcgagcagca actagaataa tttaaacatt actaaactgt ctatattgtt actaataatt
     3661 cccttcttat agtgcatttt cttagaaaaa aacaatatta cgatatcatg atattaagat
     3721 atcgtaatat tatacaattg tgattgatat tatatatgtt tttttatttt tttatcatgt
     3781 ttaatcttga aggaatcata ttctttttct aaagacagga gcttttcata aacatcagac
     3841 atgtttaaac tagaggccag gcttaaatca taaacgaatg aagcaggtaa tgattttatt
     3901 ctacgttgtc gggttatcca gcttgcaacc gttccttgtg ggtaggcata taactcacaa
     3961 aattgttcta cagtgattcc taaattcttg ataatgtaaa cattgatcgg gtgcgagtaa
     4021 acaaatgtat tgatagacat ggtgcgtaag cctactttct tttattattg cgtttgcaat
     4081 aatagtttaa cacacgcatt attctagtat ctactcctat ttgtaaaaac aaaaaaggat
     4141 tcatagagat aactctatag aatccgtaaa ataccttata tatataacgg gaagatataa
     4201 aacctaaaat tatgataacc tgctgcagct tctacataga aagagaagcg ctcaacacca
     4261 acctcattta tcaaacgttg gccataaccc caaatctctt ctgatagctg atcaaaggtt
     4321 atatacttta gctcatattg ctggataatg ttcattctaa tcgactccat ttattatcct
     4381 ggtaaaattc gatatgacgt atttcgtcgt agtgaatttt gtcttttcca acgtagatcc
     4441 ccagctcatc actacctaag atcttgccaa tgatatcttc tggatagtat ccttcagcgt
     4501 ttcgttcttc tttttgaata gatactgatc tattttttat gactgcagcg ttcaatactt
     4561 ccgcaatctc ttcagtcgtc atttgttctt ttgctaaatt ttgactggtt cgttgttcat
     4621 tgtcggcatc aatttcttca gtatgatcag ataaataaaa ccctgcccac tttaaacgtc
     4681 cacgatcatt gtaatcaatc atttttaatt cccccatttc cgcccgcatg accaccaaca
     4741 agtgtgcttc tagtaatgct tctagcgcct tctaacattg aagaagcatg tacaatcgac
     4801 gtaaaaccat atttttgccg aatcttgtca ataattttat ctaaattttc ttcgtcaatt
     4861 tgcttttgtg gatcactaaa taaatccagc tgaagcgaat cggtgtacac aagcttgcta
     4921 taggttattc caacatgcct tacttcttgt ccttcataat acttatcaaa caagaaaaga
     4981 caataattct gcaattcctt tgtattatct gtgataggga tcttcatctg gtgtgaaaac
     5041 cctggtctcg tttctaagat cgacgtgcca atgtgtaaat ggacacaacc tgttttacag
     5101 ttatgctgcc taatgcgtat cgctacttgt tccgccattt cttttaccac cagctcgatt
     5161 tcatttcttc tagtataatc tcgtggtaac acttgtgaat ttccatatga tttttccttt
     5221 gtcggttctg gtgggattgc tatatctgtt cgatcgatcc cattagcatg gaagtacagc
     5281 tgtaagccaa tcacacctag tctattctta atcgtataag gattccagtt agcaagatct
     5341 ctaatgctca taatccccat ctgattgagt ctttttttca ttcgagagcc aattccccaa
     5401 aagtccgtca tttcaggaat attccatact ttttcaggaa cgttttcgta cgtccattcc
     5461 gcaacaaatc cttcattatg ctttgcttcg ttgtctaagg ctagtttagc caaaagggga
     5521 ttatctccga cacctactgt agcaatcaat cctagctctt ctttaatatt ctcctggatc
     5581 atttgagcca gcttctttct ccgttgactt cgtgttcctt cagtcgtaaa aagattcagt
     5641 gatcgggtca cttttaagat tgattcatcg atcgagtaaa tcagtagatc ttcatcagcc
     5701 acatatcttc tgaaaatatt atttacctgc atatttcgct tgatatatag gttcatacgt
     5761 ggtggaacaa cgtgtagtgt tttaggaaat ggttgtggta agtcacgtgg tctactcaca
     5821 tttgtaatac cataccgctt ttttgcttca ggagaagaag ctaatatcaa tcctgaacca
     5881 gtattatcag ctcgactcat aacaacaagt tctgttgtta atggatctaa atttctttct
     5941 atacattcga cacttgcgta aaagcttttc atatcgatta aaaagtaatc atttactggc
     6001 tcttttgaat aatccatcat aaataacact ccattctttc acattataca aacatatgtt
     6061 cgatattgta aagaagaaat agctaaatac ataaaaaaag aagcataggt atgctatcac
     6121 cctgcttctt cttttgattt gtatgatcaa tttattctat cactttacta attaatttga
     6181 aaatctaata aaaactctca ctctaaaaga gtgagagact gcaaattaga gtacggactt
     6241 ttttggagtt ccgttcttta atattaacaa atgaataaaa tccttacaac atttgagaat
     6301 aagttatcag tagacactta caaaaaattc tcatactatt tcctccatca ttagtttttg
     6361 tctatataag gtttacccta aatagacact ggtataacat tgaaaaatat ggcttcctcg
     6421 tgtatgatta gggtgtattt taaatagttg cagaaagtag gtaaaagttt tgagagttgc
     6481 atacatacgt gtctcatcga tcgatcaaaa tgaacaacgc cagattgaag aaatgaaaaa
     6541 attcggtgca gagagaattt tcatcgagaa acaatctggc gcaacgatta ctcatcgacc
     6601 tgttttccaa gaagcattag attacgtccg agatcaagat atttttattg tggaagctat
     6661 tgatcgactt ggaagaaatt atgacgagat catcgcttca gtcaattact tgaagaagaa
     6721 aaatgtgaag ctgatcatta ctagtttacc gatcatggct gaagcaattg gcaatcctct
     6781 cctggataaa ttcattaagg atctgatcat ccaaattttg gccatgatcg ctgaacaaga
     6841 acggactgaa tcgaaacgtc gccaggcaca aggaattaag atcgcaaaag caaacggcgt
     6901 ctacaaaggg cggcccaaat tatacagtgc agacgcaaaa gatccccaac gtcgccttgt
     6961 ttacaaaagc atcgttgaag atcttaaaaa tggtgttgcg attgctaaga ttgctaaaga
     7021 ctacaatgtt acgagacaaa ccgtgtatag aataaaaaaa gacagcatgg tcaatgacaa
     7081 atagttgatc atgctgcttt tattatcttc tcatctttaa tttcctacat ataattacta
     7141 tctataaaat taagtcttac tcagaacaga taaagcataa aattccaaca aaccattttt
     7201 tacttaacta aataaagagt ctgttaatat aatcttcact tatttacgtt aatcccctac
     7261 tgtatttgtt ggttccttat ctatattttc tacaaaatcc ttattatctt gtgatttagc
     7321 ttcgtataat tctttgtatg tcgacacatt ggttatacta attcctatag ctagttcttg
     7381 agactctact tttctctcta gctcctttga caaagtgatt tgagatttat ttggttccgt
     7441 tggagtcaat tctatagatt cttcataaaa atctctatac ataaagctat ccctgaataa
     7501 ttcatagctt taattctttg gcacatttta ttgaaatatg tatcactaat atccctatca
     7561 actgattttg actaaaaagt tacttcaaac attcactgtt caaaaatatt gaggaataaa
     7621 ttttgggtgt aaggcatttt gagcatactt ctccctggat aacttcccag taaaaaaatc
     7681 gttagattgt tggattagat ccattgactg gctcgcctca ggcgagcaaa gaccctgtag
     7741 aattcattct gatacacatg ataactagat aatttgagat agactcgtct acccgtttgg
     7801 actaatttcc cagcaacttt cagaaacttc aatcgaatag aatgaatggt cattcccttt
     7861 tggacttctt caaagccaat cgttcttaag aagttgacta agttgtaagc tatcaagctg
     7921 agcatcattc ggacatgatt ctccaaaaag cgaggactgt ctgttttgtc aaagtaaaag
     7981 ccagctttcg cttctttaat gaagttctcc attgtgccac gtttggcata gatagaaaag
     8041 atggtatcag gagaaacatt ttctgataga ttcgtcacga taaattcatg tcgaaagagt
     8101 agttcgcccg cttcacgtgt tgagcgtata cacacgcgac gactttgtga ccaggattgt
     8161 gcttgataag tggtcgagaa gtactgaact tctcgttctt cccacttttg attatcgcca
     8221 taaagtactg atttctcagc tatttgacct agtctacgat tattcttcag tcgaataaca
     8281 taatgactct tttttgattc acacgaatca tacacaccag gtgtagcgaa cccgctgtct
     8341 ccacgaacca agatgtcagt gtttggtaga gagtgattat agtgctctaa taaaggtgtg
     8401 agaaactcct ttacgccttt tgatgtatat tgatttcctg aacgtagttc agcttttaag
     8461 aagtcaccag tcaatccgtc aaaagctacc aatggatggt aaccataggt ttggtagtgt
     8521 gtattataat tcgtttgttc ttggtgacca aatgtatctg aatgggtcga atctaaatca
     8581 atgattaatt ccgtatcatt acggatgagg cgtgctttat cgataagttc ttggttcaaa
     8641 gcttgaagtt catggatatt ctcctcagat agtcgatcta aaaatcggga aagtgaagat
     8701 tgagaagcga gttctttcct atctaaaaca gctttaaaca caggatcttg tctcaggaga
     8761 ttagctgcag aatcagctga gtaaccagca attaattgca taatgaactg ctcaagtatg
     8821 gataagttgt catgagtaaa atacgctcgt tcgtcttcaa tgtgaatgtg ttgcttagcc
     8881 agttcagaaa aaccaaagat gttcatcagc tctttcacta ggacgagacc cgaatcactc
     8941 gataattgac cacctgtatg agaaatagtg atatttgaat tgaattttac ttggtttttg
     9001 tgtaagctag tcatgaaaag aactcctttc ttttggttgg tttagtcact ttaaccatag
     9061 cagaaagggg ttcttttttc atcacttaac gggtgaagat gaaaaagtaa ttgtaagctg
     9121 ccgtgaggca gtttcacatg gttttaatta aaagtatgaa ttattcaggc tatcattaat
     9181 tttataaaaa aaattaaatg tttttagcac tgccatataa cattcaaaac attcttctat
     9241 agttggttta cttccttgag gagaatgaac caatttatta cgtttttcta tgaaattttt
     9301 aatttccttt cttatttctt tcggaaactc aatattaaag taccctttaa acatgctccc
     9361 taataatttg tctacagggg gagattgtaa attctcaaat attagagtta aatcaggttt
     9421 ctcttgttga taaaattcct ttacccctat ttcacaggct gtaattatcg aaataaacct
     9481 atctctagta tttgatatat ttttacttct tttaaggagt ataagagaaa aggggatact
     9541 ttcgtgtcca tgcatgtaat taactaattg ttgagcttca gaagttatat ctctaattat
     9601 atagttaacg gaattaggaa gtgtctttaa ctctaatgaa atttctatcc aattactatc
     9661 ttctaatctg tatatgctcg aagtataatt tgattcgtca gcaagcatcg ctacatccca
     9721 aacactgatc ataaaatctt ttaaacgttc aatcatttca gcaatattgc taaaatcagc
     9781 taatttttct ttagaataaa aaaaaacatt tatatcagaa tcaataaatt ctattttttt
     9841 attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg agtggggagt
     9901 acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat atataaaaat
     9961 tacttaactg tatccaaatt taatatgtaa ataaatcatt aatttttagc taaattctat
    10021 gagaagttta tttttgttgt ttttgctaat taactaaaac tagtacaaaa aatttaaaat
    10081 agattattaa acctattgct tattctattc agtcatatat ataagcgtca tcctttagta
    10141 actaacatcc cacactacaa tagtattata acatatctat tcaacttcaa attgttgaaa
    10201 tttaagccat ttatatttac ttttcctcaa ctttagaaat gattttacta accttatatc
    10261 cgtaatacgc gtaacaaaca cattcattaa taacaatcaa cactaagcta tattagcttt
    10321 ttaaatgact agggtaaata aaataaggat caaaaacttt taagtttctg accctttcct
    10381 tacttccgat tggttaatag tgatcttttg cagaataaat aaatatcgtt tcattttcaa
    10441 ctctatatat cagtctatgt tcatctgtaa ttcttctgga ccattttcca gataaatcat
    10501 gctttaatgg ctcaggtttt cctaatccag caaaggggga acgatcgata tcttttatta
    10561 acttgttaat cttttttata ttgcttttgt ttccttgctc atgccaataa agataatcat
    10621 cccaagcatc atcagaccaa gccttaatca tcagattcaa cctcgattaa gtcatgtgtt
    10681 ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa ttttttccat gacgtaatta
    10741 ttagaaagtg ttctcaacgt ttcttgcata gaatcataat ctctttttga taatacaaca
    10801 actgtatctt ctacatcttt acttgttaca ataagtgttt cagcatcctc attaacttgt
    10861 ttcatataac tacgtaaatt ttggcggaaa tttgaataag ctactgcttc cattcctttc
    10921 accccttatt tatttctata aaacaattgt acattatatt gtacaattaa gcaaatgatt
    10981 aatattctaa cttcttataa ttaaaagtgc tatcactttt agtttttagg ataaaaagtg
    11041 acgtcacttt ttgagtgctg gtacctcaaa tcctgctttt tcttgggcaa tcaataaatc
    11101 taacgcaata cacataatcc tggattcact tttgccgtac ttctgcttta gttgctgcaa
    11161 ttctttttgt ctcttttcat ccaacgtaac gggtaaccgt ttatttccta ttgcaattgg
    11221 cattagtact ctctcctttc atcgaaataa ccctatgaaa aatatggctg caatcatctt
    11281 gaatagtaga ctcacaaggg ctaatacaaa gcgccaaaag atcaaacaaa ataccagtat
    11341 tgccaaaaat actacgaagt ttaaatgcac agctgcagca tggcgcataa gtatttcgcc
    11401 aacaaatagg actaacaata gaggaatgat tttattcata tggatctcct tctttctctt
    11461 aggttatcgg gtgttgcccg cttatttcgc accacctaag cggcgggggc ggttggagcg
    11521 attggtgcga aataagaacc attcccttag ttactaggtt ttttgtcctt atttcacaca
    11581 ttctcacttt tcggtataaa tgattattgc ctttttttcc tttcaaatcg ttgatatggc
    11641 attcgtttac ggtcaagttc attttggatt tcttctgtat cgtcgtaagt ataggtgtct
    11701 aaaaaatcag ttaaagtcgt gtaccagtcg tcgcctgggt tatctacttc ttctacatat
    11761 tccctcaaaa atgcttcggg tacagcgagt tcataatcca ttcccattaa cacgatagat
    11821 tccgttccac acgtcaaaaa tctagtaaat acaaggtaat aatcgtctat cgtcgcatct
    11881 ttgctgttta gctcttccat ttcttcttcc attaattcca tgctctcaaa aattgaataa
    11941 tattctgatt ctgagtaata gtgtttcctg caaatcgtgt agctgacaaa gttttcgacc
    12001 agctcactcc tttctaggta aacattcatg atgtattttt ctgtttctag tcgtagaagc
    12061 tccgttcctt tttttaccgt ttcaggacga cgtttataca cgtctaaacg attatggctg
    12121 actgtgacct tcgtttgact cgtttcactt tccaaataca attgttcttc
//
LOCUS       DAYYCQ010000083        12044 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000083 DAYYCQ010000000
VERSION     DAYYCQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.084"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..362)
                     /locus_tag="ACHAYF_002315"
     CDS             complement(<1..362)
                     /locus_tag="ACHAYF_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8579731.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLT"
     gene            1056..1442
                     /locus_tag="ACHAYF_002316"
     CDS             1056..1442
                     /locus_tag="ACHAYF_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8579732.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1536..1727
                     /locus_tag="ACHAYF_002317"
     CDS             1536..1727
                     /locus_tag="ACHAYF_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579733.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2241..2573
                     /locus_tag="ACHAYF_002318"
     CDS             2241..2573
                     /locus_tag="ACHAYF_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8579734.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2483..2677
                     /locus_tag="ACHAYF_002319"
     CDS             2483..2677
                     /locus_tag="ACHAYF_002319"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8579735.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            2716..2862
                     /locus_tag="ACHAYF_002320"
     CDS             2716..2862
                     /locus_tag="ACHAYF_002320"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579736.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            3300..4640
                     /locus_tag="ACHAYF_002321"
                     /pseudo
     CDS             3300..4640
                     /locus_tag="ACHAYF_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4881..6497)
                     /locus_tag="ACHAYF_002322"
     CDS             complement(4881..6497)
                     /locus_tag="ACHAYF_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8579737.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6615..6833
                     /locus_tag="ACHAYF_002323"
     CDS             6615..6833
                     /locus_tag="ACHAYF_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579738.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            7028..7489
                     /locus_tag="ACHAYF_002324"
     CDS             7028..7489
                     /locus_tag="ACHAYF_002324"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8579739.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7682..7921)
                     /locus_tag="ACHAYF_002325"
     CDS             complement(7682..7921)
                     /locus_tag="ACHAYF_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579740.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7945..9468)
                     /locus_tag="ACHAYF_002326"
     CDS             complement(7945..9468)
                     /locus_tag="ACHAYF_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8579741.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9486..9608)
                     /locus_tag="ACHAYF_002327"
     CDS             complement(9486..9608)
                     /locus_tag="ACHAYF_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8579742.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9638..10621)
                     /locus_tag="ACHAYF_002328"
     CDS             complement(9638..10621)
                     /locus_tag="ACHAYF_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8579743.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10645..10794)
                     /gene="rpmG"
                     /locus_tag="ACHAYF_002329"
     CDS             complement(10645..10794)
                     /gene="rpmG"
                     /locus_tag="ACHAYF_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8579744.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10815..11192)
                     /locus_tag="ACHAYF_002330"
     CDS             complement(10815..11192)
                     /locus_tag="ACHAYF_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579745.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11224..11403)
                     /gene="rpmF"
                     /locus_tag="ACHAYF_002331"
     CDS             complement(11224..11403)
                     /gene="rpmF"
                     /locus_tag="ACHAYF_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8579746.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11418..11687)
                     /gene="rpsN"
                     /locus_tag="ACHAYF_002332"
     CDS             complement(11418..11687)
                     /gene="rpsN"
                     /locus_tag="ACHAYF_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8579747.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT         3866 a         2288 c         1861 g         4029 t
ORIGIN      
        1 aaagtcaaat tttgcaatga attatagagt cgttctctat cagccattgc aacattcaaa
       61 ttgttcattt ttagtttaaa aaaaacttta tcaatattat ttgctctaaa tggaccaaat
      121 aagtaatatc tggttttaaa catgtataga agaaacatct cattaaaatc accagtaaag
      181 aaatggaaat ctttagtttt tttaatatta tcagttagta actttttata atcataaaaa
      241 aaataagtag ttctatctga gcgatactct tttataatac tgaattcgga attaaaaacc
      301 acgataggta actttaaaca aacatgaaga gactttaata agacttcttt atctgaaaac
      361 acttttgttt ctctctttcc tttctacatc attaaatagt attttatacg ttatctaaaa
      421 tactattcct ctgtcaaaat aagctaactt acttcattat aaaatatcca cttatcgctt
      481 tcatgtatat tccaccgtaa aaagtacatt ttatcaccga gaactcatca tttttcatca
      541 tgatgaatac tatttacata tatctagttc aaagacaata attattatga tccgttatta
      601 tctaagcact atacagataa taggaatttt tcgtttctaa atattccaat ttgggaatcc
      661 aataaaaatt ttcgttcaaa tacgtgtcta ttcgaactct tttctagttg ttaattatga
      721 agggggaaga aataaaaaat acaatctata cacgaataag aatattatcc aaaaggaggt
      781 tttagaaaag atttattata cgtaaaaagc tcaagacatt ttcttagcca taaggacatg
      841 tttgatattt aagaagatat atgaattttc ttttctaagt ataaagataa ctatccatta
      901 tcaactaaaa aaacattttt gaaaataaac atcagcagtc ctatcaatgc atgtgagctt
      961 atagaagtta ttcgcagcgc agcataattg cattagcttc ttttcgtgtt ttttgcctct
     1021 tttcccttta aatcttaatg gaggaggtgt gcaatatgtc agataacgac gaacgcgtaa
     1081 atgcccagtt gattcgattc tttgaaaagg tcattttgaa caccgctaat acttattata
     1141 aaaaacaaga agaaattcaa aaacatgaac aacttgatca actcccacca tatttgttta
     1201 ctaaagatac tataaaattt aaacaaccga ttaatgtttt aaatgtgact ctccttgttg
     1261 aagatataaa attagcaaaa atccttcctt tgttgaaaga acctgaacaa atttttctta
     1321 tcgaaaaatt tatctttgat aaaactgata aagaaattgg aaaggttctg gggattacaa
     1381 gacagggtgc gacaaattta aaacatcgac tttacaaaaa aattcaaaaa atgctggttt
     1441 aatatctgca taataactaa ttttgtatgc ttctttgctc gttctcctcc ttcccaaaga
     1501 aatagattat aagtccatgg gaagaaggta gatttatgaa ttctttaaaa gaagatttca
     1561 ttttagcaaa ggctggaaat gaagaagctg ttgaggcaat tctcaaaaga tttagctctt
     1621 tgattcataa gcaatcctgg agaactggaa aatatgatca agattgttat caagaatgta
     1681 tgcttgcaat atatctagct atctcgaaat tcgaaattaa agaataactc aactaaggga
     1741 gtctgtgaca taagtaaaaa gatagtttca aatttcgaac aataaatttg aaactatctt
     1801 ctttataatt ataaagtatc gattttacag cgttctataa tgattcatag aatctaaaac
     1861 gaatgatttt aattatcaac gttttttaat tcgtttttag cttaaaaata acttttgtca
     1921 cagtctcact cctaaaatta ctttatttac gtatgaagtg ggcaggaggt cgaatccaga
     1981 actgggctat ggtacttcaa cgaggacttc tcctctagaa ttgaaaagca tgcgatttac
     2041 cttccgtaac gacggtagat ttattcaact atgtgtccaa tcaaaatttg tctgatttca
     2101 aaaatagtca agagaatttt ggttgttaaa aactcttatc tatcaacttc cacagtttag
     2161 ttgacaaacc ctgatactaa gttgggctat gatacacttt tcttgactat cagttacgtt
     2221 accggaagga atgaactcta ttgagaaaaa catatgatcg taaatttaaa ctaaagattt
     2281 cacaggatat ccttgagaaa aaagtaacta ccaagaaaat tgctgaagaa tacaatatct
     2341 ctcgtccaac aatttcccga tgggtttcag aatatcgtcg atacgggaaa aatgcttttg
     2401 ctggacaagg aaaaagatta cctgataaag cagatttcta cattcttgaa caggaaaata
     2461 aacgactaaa agaggaagac gagtgttcta cgaacacaaa gatcgatacg gatctccagg
     2521 gatcactcaa aaactttacg atgaaggaat cgaaatcaat aaacgtgttg tagctgttct
     2581 tatgcgtgaa atgaatttat gcacaagagg atttcatcga cgcaaatctt ctcatggtag
     2641 agaaaaaaca atcaaagaaa tggtaaaaaa atgttgaatc gataatttga acagaaccaa
     2701 attgatgctg tttgggtgat ggatattact tacattccat gtaatgacgg tcgcctctat
     2761 ttgtccacct atattgactt agcaactcgt attctacgtt actatatggt agattcacac
     2821 atgaaaaaag agattgtcgt ccgtccatta caaatttatt aaggagaatt gcctacggta
     2881 attcattccg atagaggtag ccagcatcgg tcttttattt atcaagagct gttgaacgac
     2941 caccacatca cacattcagt gagccaaact gggactcctg ttgacaatgc agtaattgaa
     3001 gcgtttcatc gatcaatcaa atgagaatta atcgactcca acaaatataa aagtaaaata
     3061 gagatgaagg tgttaatcca agattattta gaagactact acccaaacaa aagaatccat
     3121 acaaaattta tgatgactcc ttgacaattt gaagcgaaga aaataaggga tctataatat
     3181 ctagtgttgg tgacgccttc tgttgccaat actttttctt ttttctaata aacgcaaaca
     3241 gacaccttaa aatcctttag aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga
     3301 tgtcctattc agagtttacc aaagagttac tagatattct agacctaaat cttatctttc
     3361 atgaagatgc ctttcgcaaa gagcgaatca atgacgaaac atgttttgtt tttgatggca
     3421 cattgaccta tcaaccggag gaatgctttc atcgccacta tcaaaatgaa caaacgatca
     3481 ttaaataggg ctggaaaaaa gtatccatct tattgaatga tgtcagtaac tacaagacca
     3541 ttctgcggat taacaaacag cgcttcaaat gtaaacactg tggaaagact tttttagcag
     3601 aagattcagt tagtgatcgt cgctgctcca ttgctcgcag agtcaaacaa gccattcttg
     3661 agctattaag cgaacctctt tctatgtctt tgatcgcacg tatgaagcac atttctccga
     3721 ccactgtcat tcgaatactt cgcagccttc ggcctaaaac catttctttg agccagctgt
     3781 taccagaagt ggtgtgtttc gatgaattca aatcagtgaa aaatgtctcg ggagccatga
     3841 gctttgtcat gatggacggc aagacccatc aactgattga tattgtggaa aatcggcaac
     3901 tcaatccttt acgcgactat ttcttgcgct accctataaa agtccgtgaa caagtgcgtc
     3961 tagtggtttc agatttctat acaccctatc gcacattggt gaaagagtgt tttccgaatg
     4021 cccgaatcgt cgccgatcgc tttcatatta gccaacacat tggtcgggct tttaccaacc
     4081 accgaattca agtaatgaag acctttaaaa aaggggatcg tcggtcgaag cacttaaaga
     4141 aatactggaa actacttcaa aagaatgcgt gggaattgaa aggtcagcat cgctactggc
     4201 gtccgtcatt tcgagatcat ctgacagaag cagagatagt cgatcggcta ttatcttatg
     4261 atgattcctt aaagcgaggc tacgaagtct accaggattt tctttccgtt atcagaagac
     4321 aagatgttcc tgaattcgtc gcgctcctaa aagaagacta caaggagttg ccagagcact
     4381 accaaccagt gttcacgacc ttcaagaaat accaaacaga aatcaaacga gcgttgcgcg
     4441 ttccctattc caacggccct atagaatgtt tgaataacca tatcaaagta ctaaaacgaa
     4501 ttgcctatgg ctatagaaac tttcaaaact accgagaacg aatcttttta tatcgaggaa
     4561 aatattttaa gaaaactaag aatactaccc aactgactaa agccagaaca acaacacggc
     4621 tagacaagat agctgtctag ccgtgtgagt atctcgatta ttctattgaa tttcttcacc
     4681 aacactcatt gacgaagagc caaaataagt aatctaaccg actgttaact aaaccattgc
     4741 aggtcccgtt aaagatactt gtgcctgttg tcaccgcttc tttttcttgt ccacaagcgc
     4801 ttaacacact taataaagcc cctgctatta ttaataataa tatctttttc ttattaaaaa
     4861 cctcctcatc tatttccaga ttagtaaatg tagtaatctg tgctttcttt tttctcaata
     4921 ctaataacta acttatgggg taaacaaata ctcgtttgac cgggttttga gatccatcca
     4981 gttctaaccg cgatttgatc aggactatta tcttctttaa ctcgaattct tccatcttta
     5041 atctcaacaa tattatattg tcccttcgca ggatgataat tcacgagttt atgattgatc
     5101 ttatccaaat cgaagcgatc catttccttg ccatcaattc gaacgatggc atatttgata
     5161 gcatcttcag ttgtttcatt ctccttttct atagagaata caaataaggg aataaaagag
     5221 gtaataatca aaatgccaat aattatgaca tcgcctatct tgacaccagc ttttcttaaa
     5281 aatttcatta acgatatctg gctaaacata ttagactttt tccatggaaa tggtgaaatt
     5341 tttcggaaat tatctgcatt aagagaatca gttaaacaac cctcaagttg ttccttaata
     5401 cattgttgat ccatcttcat accaataaaa actagtttat taatgcgttc accaaacaac
     5461 ggatgccagg cttccttcaa ttcgggattt ttttgtataa tcatttgaca ctcaatttta
     5521 ggtaaactag ctacccaata atttaatgat tcaatacttt tatagctacc cgatgtttca
     5581 aagatcgtcg aaaaatcact atgttgggca aaccaaagaa acccttttgc tcttgtaata
     5641 ttgccaggaa aatttttatc cgtaagatgg gcaaattttt gtgcgtcaag gagtaaccga
     5701 tttcgataga taaaacttga gataccatat tcttctgttt caggaatatg attttgatag
     5761 ccaagctcta actctttgaa ccagcccact gaataggaag tctcatctat attaaaaaga
     5821 tttgtattta gaattttcga taagtctact tcacctttaa ttgtcctaat tattttggca
     5881 ttggactgta acttagcaat tagaccgtta attttgtaaa tcgtcttggt tgacactaag
     5941 tcactattat ttaaaagtaa tatgttacaa cactcgaatt gatcaattaa caagccacga
     6001 atatcctgtt cttcttcaac tacattacct tcagcttttc tttcctgaat cttttcaccc
     6061 gtttcgaaat tatcccaaaa acgttttaca tccactacta taataatggc accaatctct
     6121 aatcgttggg tcaaatcaat gtctaaaggg tttttagcta atataattgc ttgaataatt
     6181 ggaattggtt tgctgattcc tgaagcttca ataacaattt gattcaagtc agaaaattgc
     6241 actaattgat ctagttcaac aatcaaatct tcgcgtaatg tacaacaaat gcatccattc
     6301 ggcatcgaaa caagtttttc tccagttctt ttaaaacctt tcttgtcgac taatacccca
     6361 tcaacattta cttcccctat gtcatttaca atgacagcaa ttttctttcc gtgatcaccg
     6421 tacagaatat gatttagaag tatcgttttt cctgaaccaa gatagccagt taagattgta
     6481 ataggtattt ttttcatagg gaacctcttt ctataccttg aatttacact ttaagtgcaa
     6541 cacataaaaa tgagtcataa tcgatatcca actagtataa gtttgtcgag aacactccta
     6601 gaaggaaaat aattatgagc cagtatcgtc atcttactat actcgaacga gaaagaatct
     6661 ttctgcttca cgaagaagga ttttctatta gtataattgg caacgacatt cagcatagcc
     6721 cgtttactat tttacatgaa ctgagaagaa acaagaaaaa aactattcac cttccaaagc
     6781 ggaaaaaaat tatgcttatc gaagaaaaaa atgcggacgt tatcacattc taaatgatcc
     6841 gtgagtttgg tcaattgttc gtcatttatt tgtcgatttt cagtggtcgc cagaggaaat
     6901 ctcgaatcgt ttgaagcatg aacatatgga cttttcaatt agttttatca cgatctaacg
     6961 tgcaatttgc aatgatcttt tcgatacaga acccctctca cacggcaata aaggcctgat
     7021 ccgttcattg caccatcgag gaaaaacaag acatacaaaa aatcatgtcg agtctcgtgg
     7081 aaagatttgc cttactcatt cgatccatgg ccgtccaaaa tccgcagatg atagaagtga
     7141 ttttggtcat tggaaagccg atattgtagc tggcaaaact ggtggtactt gtttagtgac
     7201 tttgacagac agaaaatcat gcttcctttt agcaggaaag aaggtcccca gaaagaaagc
     7261 cgcttatgtg agcgagaaaa tgattgaact tttaagcagt attccaaatg aggcggtcaa
     7321 aacgatcact ccagatagag gaaaagaatt ctccaatcat ttcgcagtta cagaagcatt
     7381 gaataatatg cccttttatt ttccagatcc tcatgccctt ggcaacaatg aacaaattaa
     7441 aatacgaata gactcattcg agaatacttg ccaaaatcga aagagatagc tacggtagat
     7501 aactcaacga taaatcagat gattgaaaaa ctgaataaca gacctagaaa gtgtttagat
     7561 tggagaacac cttatgaagt cttctttgat aaagtgttgc gcttgatttg acaattcaag
     7621 acataataaa aagcagatca aatccaaata agggaaaaat gatctactct ggtattatca
     7681 attaattggc caagctatta tgccgttttg ctaaacgtct acttcttcca gtattgacct
     7741 tgataaaaca acaatcagga tcccgtttta atactctatg aactgtgtca atactaacat
     7801 gtaaagttgc agcaatatca gctaatgtac tcccatttaa tctcatttgc tttgcaaatc
     7861 gataacgccg ataagcatca atattcttgg aatccataga caataatact tttcttacca
     7921 tgtatttcct ccaaaaattg aattttaatg agccatcatt tcttgagcaa tttcatgttt
     7981 agacaatgaa gctggataga aagtaggcca attatgcaat tcattataga gtttttcttg
     8041 actttctcca ccaaagaaaa tatggaaatg ctctgcctta cttggtgcaa ttccatgatc
     8101 actaaattga atatatttaa aacgaccagc acctgcatca tcagtttcaa aattaaaacg
     8161 aacaccacgg tttccttttt cataattcaa aattttgtat ccaacatatt tgtatgtgta
     8221 ttgatgatgt tcaccgttga ctacaaaatc aatggtgttg tctttaatgt tgatagtatc
     8281 aatatctgtc ttatagccag tagtataata atctttgtac tcggcagctg tcatgtcttt
     8341 attgattttt gctttgtaat cgaaaacttc atccaaaatt ccatctttta acaacggata
     8401 aacagattgc cattcgcctg tataatcact caattcgcga tccttcacat cggcatcttt
     8461 aaagtaccca ttagcaactg tcttttcatc cttcgcttct ttttctggaa ctacctcttc
     8521 accagcaacc atagttgttt tttctaacgc ttttagattg tcttccatta ctgaaacata
     8581 atcttcacca ttatccattt gttcttttgt aagactttca agaggattca gtacttccaa
     8641 ttttatccca gcttcgttgg ccaaagtttt tgcaatttta tcattagcat tcttttcaaa
     8701 gtaaatataa ttgatcttat tttttttaac atactctttt aattcagcta atcgaccaga
     8761 agatggctct tcttcaggac ttagtccagc aataggaact tgtttcaatc cataatccaa
     8821 agctaaatat ccaaaggctg cgtgttgcgt cacaaaactt ttttgtttcg cttctttaag
     8881 actagtggta tagtcagcat ccaaccgttt cagtttagtc aaatactttt cagcatttgt
     8941 ctcaaatact tttgcttttt tcggatacaa tttaactaac tgatccttga tatttgaaac
     9001 ttccttaatt gccattttgg gtgaaaccca cgtatgagga tctagttcat gatggtgatc
     9061 ttcttctccg tgatcatgat cttcttcttc actacctggc aataaaatca tgtcttttgt
     9121 tccttctact acattaggcc cagctttctt ccaactttct ttagcttttg gtacccatga
     9181 ttccatgttt tcgttatgat atacaaaaac atcagcatca tgagcttttg ccatatcctt
     9241 agctgagggc tcatagtcat gaggttctga tcccgctgga accattaaat ccacgttcgc
     9301 ttcatcgccg acgatgtttt tggtgaaatc atacattgga tagaatgttg ttacaatttc
     9361 taattttcca tctttatcat tcgatacttt tgcatcatta gtgctggagc aagctcccaa
     9421 taatcctaga gctgctatcc caataagtcc tacactgatt ttcttcattc tttttccttc
     9481 cttaattatg ctaatttctt cattttattt cgtttatgtt ctttgataat ttttaatgcc
     9541 ctttttctag ttttaatatt cgggctttta agtcgtcgat atgcactaga tatatccgtt
     9601 ttctccatta gaaattaccc ccaatcacta cttatcatta tttgctttcc gccagtaatt
     9661 tatcaaaact ctgtttgatc ctttcttcat ccaacatttt tccgattaga ataatagtgg
     9721 tttggtcttt ttgtcgagtg gtgtattgaa aattaacctg ttcgtttacc tcttgcatgg
     9781 caactataaa atcctgctct tgtaaagcaa tcaatccttt aaaacgatac aggctccctt
     9841 ggttggttaa aatcagccag tcaatccatt ggagtaaaaa atcttttttt atgtcttgat
     9901 ctgaagttaa gagaattgat ttaaactcat gtacttgatg gtgttgatga tagtatttgc
     9961 cattatagtg attttctgat gaatcacttt caaaaactaa gggatgatta aattttttca
    10021 aattataaaa atcctttttt tgaatcgttt cactatgtgt gaacattcga atatcggata
    10081 aagggttaat acttttgatt tctctctgta atttttttag tgcagataaa ttcaagtttt
    10141 gttttctaga cacaaaaatg cgatctgcca tgacaagttg cttaagggct tgtggttctc
    10201 tttcaataag ttcaaaactg tcactatcaa taacagtcaa taaactatcg atatatactc
    10261 ttcctcttag ttgaggcgct gttaaaagtg tttgaatgat tggttgggga tctgctatac
    10321 ctgtagtttc aatgatgatt tgtgaaattg gttgttgatg ttcttctatt gtttccagta
    10381 aagaaatcaa tattgctaat aaatcactgc gaagactgca acacatacat ccattattta
    10441 gttgtaacac ccgttcctcg ctatgaacta ataatttgtg atcaatccca acttcaccaa
    10501 actcattttc aattataata atttcttccc tggggtaggg tgagacttgc aaggcttgat
    10561 tgattagcgt tgtttttcca gcccctaaaa acccagaaac gattgtaact ggtatgccca
    10621 ttttgtcacc tttttaccac ttagctattt cacttcttta aaaattgccc gtctgcgcaa
    10681 ttttggcgaa tactttttta attctaaccg ttcagggtta ttccgtttat ttttgctggt
    10741 gagatacaga cgttctccag tttcgacaca ttccaaaata atattttgac gcataaatct
    10801 tcctccttca atacttatcg atcttcaaga tatgcttgaa tttgttgtgc atcttcaaaa
    10861 gttaactcgc cagattccaa ttgtgaaact aaattttctt tcatatcaac aggtatctcc
    10921 caataaccat cgatacattg aaataactcc tcgtcatact cttcaaaaat tggatagatt
    10981 cttaaaaaat catcaaatga taagccttgg tcctcatagt aatctttgac taatccattt
    11041 tcaattgcct gttgggtcgg taaacccgtt tcagtaataa ttcctctctc taccatcgaa
    11101 cgataaaaag tgcatttatc tagcgattgc tgggtcgttt caagataata ctttaatcct
    11161 tcttcaacta aaggtgggta aataatttcc attttgattc cctcctaaaa gttatatatt
    11221 aaactatttt gaagctcctt taattacttt ctttccatta tagtaccctt ttaaagatac
    11281 atgatggcta cgtctataat caccattcgc ttcgtcaaat gaaatttctg gttttactac
    11341 agtttgatga gttcttctta accgtttctt cgccttagat gtttttcttg ctggtactgc
    11401 catgatattt cctcctttta ccaacttgct ttttttactc caggaatcaa cccttgatgg
    11461 gcaagttctc taaacttaat acgggacata ccgaactttc gcatataccc tcttggacga
    11521 ccatccgtct gatcacgaag cctcagacga ttcggatttg aatctcttgg caatttagcg
    11581 agagcggtgc gatctcctgc ggcttttaat tcctgacgtt tatctgcata cttttcaatc
    11641 atcgccattt gtttctttgc tttagcaatt ttcgactttt ttgccatata cctttatact
    11701 ccttttattt tgatttagac acctcaaatc gtaatcgtta cgttttgaca ctgatacttt
    11761 accaacaaga gttgctcttg tcaacaataa ataacatgaa ataaaactca tatgaataga
    11821 taactatttg aaaacgaatt tatcggtaca tagagataat caaatcattt aaaagaatat
    11881 gcagtttagg ctctgaaaaa taagaattca cttttattag tccaatcaaa cacaaatacc
    11941 gttttaagta gcttttcctc tacttaaaac ggcaatatga aagaaactat aactttttat
    12001 tcaatctttt atgtttaact ggtttatcgc caatttttta aaac
//
LOCUS       DAYYCQ010000084        12014 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000084 DAYYCQ010000000
VERSION     DAYYCQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.085"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(223..948)
                     /locus_tag="ACHAYF_002333"
     CDS             complement(223..948)
                     /locus_tag="ACHAYF_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579748.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1102..1428)
                     /locus_tag="ACHAYF_002334"
     CDS             complement(1102..1428)
                     /locus_tag="ACHAYF_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579749.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1465..1830)
                     /locus_tag="ACHAYF_002335"
     CDS             complement(1465..1830)
                     /locus_tag="ACHAYF_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8579750.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1867..2082)
                     /locus_tag="ACHAYF_002336"
     CDS             complement(1867..2082)
                     /locus_tag="ACHAYF_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8579751.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2211..2546
                     /locus_tag="ACHAYF_002337"
     CDS             2211..2546
                     /locus_tag="ACHAYF_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8579752.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2622..4619)
                     /locus_tag="ACHAYF_002338"
     CDS             complement(2622..4619)
                     /locus_tag="ACHAYF_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579753.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4717..4992)
                     /locus_tag="ACHAYF_002339"
     CDS             complement(4717..4992)
                     /locus_tag="ACHAYF_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579754.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5224..5958
                     /locus_tag="ACHAYF_002340"
     CDS             5224..5958
                     /locus_tag="ACHAYF_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8579755.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5997..7139
                     /locus_tag="ACHAYF_002341"
     CDS             5997..7139
                     /locus_tag="ACHAYF_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8579756.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(7767..8252)
                     /locus_tag="ACHAYF_002342"
     CDS             complement(7767..8252)
                     /locus_tag="ACHAYF_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM8579757.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(8254..8925)
                     /locus_tag="ACHAYF_002343"
     CDS             complement(8254..8925)
                     /locus_tag="ACHAYF_002343"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579758.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(9188..10381)
                     /locus_tag="ACHAYF_002344"
     CDS             complement(9188..10381)
                     /locus_tag="ACHAYF_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8579759.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(10465..10665)
                     /locus_tag="ACHAYF_002345"
     CDS             complement(10465..10665)
                     /locus_tag="ACHAYF_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8579760.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(11089..11322)
                     /locus_tag="ACHAYF_002346"
     CDS             complement(11089..11322)
                     /locus_tag="ACHAYF_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579761.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(11329..11760)
                     /locus_tag="ACHAYF_002347"
     CDS             complement(11329..11760)
                     /locus_tag="ACHAYF_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8579762.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         3677 a         2465 c         1942 g         3930 t
ORIGIN      
        1 aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt
       61 aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga
      121 aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata
      181 acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt
      241 aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt
      301 cccattttca agtaaccgaa gtggaatatc tttaccaact ttctctgtca ctgcattact
      361 gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt
      421 atcagtattt atcgtgattc ctttatcttc atttacaagt ttccaagtag gtggtatagc
      481 tgtgtatgtt tctacaaatg aattaagttt ttcattaagt gacttctctt ttatatttcc
      541 atctaaaaac tctgtatata tggcttgagc tatactaaaa caaagctggc cttgttctaa
      601 taattttaca tcattagtta gtaaagctgt gttcacgtta tcaatcgctt caagaagagt
      661 atctttggtg tccctaacag ctttaggtgt gccttctgga atttccctac ttgatacctc
      721 gtccatttta tcttccataa accccttaaa atcatatctt tctcccaaat ccatagaagt
      781 ttttgagaaa gctttcattt ctgagtctat ttcttcaata atatttaaca tgtctgatac
      841 atattgttta ggctgattcg tttttaatct atatgtggta aatatctcaa ttcctccaat
      901 tatcaataaa attgccaaac aaatccataa taattttttc tttttcattt ttcctcctat
      961 acactgctgt tataacagtg actaatgtaa agattttccc agcaatcatc agactattat
     1021 ttacagggaa tcaaaaaaac tccttagtaa gcttacacct ttaggagttc ctgtttgaac
     1081 ggtatgcata atacatttaa atcaatttaa cattaacacc tcagtatatg gtatctctac
     1141 atatacacgg tctttttttg gatagtactc gacatttata ttgatagcac ttccattgtc
     1201 taacgtagct ttagcccggt acgtgttatc accattatca tgagtgatcg tcacatcatc
     1261 acatgtccga tttaagttat actgttcttg taaaatctga gttaccaatg gtacagatga
     1321 cttctctagt ccagatgaac ccgtcattct tgaaaaaatt atacctataa tagccaatac
     1381 aacgatacct ataatgacaa acttcttatt catatttact ttattcaact ttttatctcc
     1441 tatctccaaa atgattttct ctcatcagta actcattgac atccccaata gtacaccaaa
     1501 gatggctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttctctcg
     1561 atataagtaa cccataataa cagcaacagc accaaatacc acaggaataa atgctaatga
     1621 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
     1681 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc
     1741 ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg caatacttac tttcatttgt
     1801 tatttcttta ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct
     1861 tattagctaa ctgtttactt tattaaatac atcagaaaac agctcattta ttggtacact
     1921 taatatctca gaaagcattt tcatttgata gggtttagga atcgttctcc ctgtttgcca
     1981 cgaacttaca cattgctgtg taacattcat ttttttggct aactcagttt gggaaagtcc
     2041 tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc cctcctgttt
     2101 tattatatac aacaatatga tgtatttcaa ctcataaaat atataaatac ttcaaatgtc
     2161 attacaaata caacaacttg ttgtactcta ctgagaaggg gggtgtatta atgaaacaga
     2221 agcaatatgc aaaggtcatt agagaaaagc gaaaagcact cgggtggaca caaaaagaat
     2281 tagctaaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag
     2341 aacctaacct cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt
     2401 ttctagacaa tgttgacata gattatgagg aagagttttt ggctctctat cgctctctta
     2461 gtactgaaga tgcaattcga acaattgact atatgaagct attaaaaagg caagaaaatg
     2521 aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt
     2581 gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa tataattggg
     2641 tctcttctat tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgataaata
     2701 ggaactaatt caactcctga taatatcaat tcttttatta cttcatctat ccctgcccct
     2761 ttcgctagat atactaaaaa actactgctt gctttcacat tgtatagttt cttaatatcc
     2821 ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttctattaaa
     2881 gcatttttca cgctgttatc cctgacattt cgtaggcgtt cacaaacagt gttaataagt
     2941 tctttttctg tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct
     3001 tcatctaaaa tcaattgttt ttccttgcta agttctgatc gggaattttg aggaaatact
     3061 cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat
     3121 gtaaaatgaa atttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa
     3181 gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgtttaaa gtttcctaac
     3241 gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca
     3301 tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct
     3361 atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa
     3421 atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca
     3481 tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt
     3541 gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga
     3601 tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa
     3661 attagcatca caactccaat aaaattttca ctgatatcac aattactgga atgacaataa
     3721 aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga
     3781 aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat
     3841 ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac
     3901 tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact
     3961 agttcatact ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca
     4021 atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac
     4081 ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg
     4141 ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt
     4201 aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc
     4261 gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct taagtatcta
     4321 ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca
     4381 tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc
     4441 ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact
     4501 tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc
     4561 ttctctaata atttttcttt atctacataa attggaatat taatcattga tgtttccatt
     4621 tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac
     4681 tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa
     4741 actatcaata tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc
     4801 gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg
     4861 ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca taggctttag
     4921 cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat
     4981 ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat
     5041 cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa
     5101 atgattacca gttcatcgac atacacataa tacctcgata aaaaaggggt gtcaatagtt
     5161 ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg aggtgtggtc
     5221 aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa
     5281 ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac
     5341 aacggtcaat taattgacaa aaagactatc tacgaatggg agaagggaac ctatttacca
     5401 ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt
     5461 tgtggcaatg tagaagaata tattttggga attattcttt atagagatag cattgtacta
     5521 gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca
     5581 gtacactcta atttagatac atggctggat agatattcca aattagaacc agagatgcaa
     5641 gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat
     5701 atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt
     5761 cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct
     5821 attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata
     5881 aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag
     5941 ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttcatgg
     6001 caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca
     6061 ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca
     6121 aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa
     6181 agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta
     6241 ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc
     6301 cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag
     6361 atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt
     6421 tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc
     6481 cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt
     6541 tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg
     6601 catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt
     6661 ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa
     6721 ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt
     6781 ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata
     6841 ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca
     6901 ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc
     6961 atgggtttag acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag
     7021 ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta
     7081 caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa
     7141 agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct
     7201 tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt ccaatgactc
     7261 ttttgggaat cagaggaaag aatcaggctt tatagagata tttcatctaa caaattactt
     7321 tagactgaca gtatatatca gcaggtactg tcatcgggaa cgtagccaat atcgtatcca
     7381 aaacagcagg tagagaagct atttgaggat tcaagttatt ctatttcaac catgaagtta
     7441 agttcattaa aaataaagat agttggtatt tcaataattt ttgtatagag ttaatcactt
     7501 tattgatgct aattcatctt gattggtatt ataatcgtat tcaagttgcg tggcgtgctt
     7561 ttgctagaaa atatataact gttctttata aagaaatata aggattaaac attttttatc
     7621 atagagtttt acttatctat agaattatgt ctgttcctaa ttcagtttga tgaattaact
     7681 gataaaaaaa tatctactat ttaaagatac agggataagt aagtaaaaaa ataacatgtc
     7741 attagatagt agacagttcc aactctttat ttaccttttt tgtatgctgc actgtcaatc
     7801 caagttccta aattgttgta tccatcatca cttatataat catattggcc atcaataatt
     7861 tcgtcaaacc acaaatattc tccatctggt ttttgcccaa ttattttaat tactgattga
     7921 ctagtagttg tcatctggtt aaaatctttt attttaccta tattaacggc caaaatagca
     7981 tttcctcgct tataactctc ctggatttca tatttaacat attctcgatc aagagtttct
     8041 ttacctatca gcaccacagt cactgatgtt ccaattaatt gattatctat ccagtttttt
     8101 atagcaatat ctccattacg tttaactttc tcaaattcag ccttatctac aaaaccagct
     8161 tcctttgttc ctcgcaaagt ccagctgttt ctaactacca ttgctcttgt tacatctgca
     8221 taatggaaac taaaaaaaac ccttctcacc atattaatcc tcctgaatac tatttattaa
     8281 actgacaatt tgttcaactt ttttatctac ttctccttca gtatctaatt tttctagcgc
     8341 ttgaaattta ctttttaagg aatcatcaaa ataatcactg aaattctctt gaatcttatc
     8401 aaacaaaacc ttacttgcac ctttggtaaa tccaatcgga actactatat tttcattttg
     8461 aacagataat tcaaattctt caatcattcc atctgctgta actatttcac ctgattcaag
     8521 tttgtttcca aaaacaaaaa tactaatccc agactctgca ataatgtttt ctcgatatct
     8581 tctccactca tgaggcatat caaggggaag tggtcttaaa gaaagtacgt cttgtatgtt
     8641 attttttctt aaatttcttc tctgctttag aactcctgaa acaatactat taccaattcc
     8701 aagtccgtaa cctgatacta ttctataacc ggattcagaa agcctatatg ctaatttctc
     8761 gacaaatttt aatcctttcg attcagatgt aaatggagta taatcaacag cacttcccga
     8821 aataaaaata gtttttctca aatacttttt tctaatactt ttcaaaatat cagtaatctc
     8881 agaataatca tctacttcaa caacgtttat cgaaaatctg gacaaatcat tgatttgtag
     8941 tcttcttttt gtcagttcat aattatatct ctcttgatca aaaaaatcct cagaatcgac
     9001 aaaattttcc tttaaaatta tatgggattt tagattatct ttttagaagt tcttgaatgt
     9061 cggtatagcc cgtgtttccg ggcttttccg gcatttttga tattgttaga tacttacaaa
     9121 tgttcttatt acctctcacc atttccccct caaaagtagt aaatggagta gtaaaaccgg
     9181 ccttgtgtca ggctgccagc cgttccattt ctgcccttgc ggaatcgaat gtggcgtgtg
     9241 cataatagtt cagcgtcatg gtgatattgg aatgtcccat aatgtactgc aaggctttgg
     9301 ggttcattcc cgcattggct aaccgggtgc agaatgtatg acgcatagca tggggcgtcg
     9361 tcgttttcgg caacgcttct tcatggctct tgttgtactt cttcacaagc ctgccaaaca
     9421 tcccgccata atctaccgcc gtcatgggat agccgttctg tttgagaaac aggaaattgg
     9481 catacccgtc aataatgaac ggctttgcac ctttgcggtt ttttatcact cgctggaacg
     9541 cctgatacac ttcctcattc atggggattt ttctgacgcc gctttgggtc ttgggttcgt
     9601 caatatagta accgacgccg gtatttctca aaagctggtg gctcacgatg attacccggt
     9661 tttcaaaatc caaatctttg tccgtcagtc cgcacagttc cgaaatgcga agccctgtcc
     9721 ccagcagaat aacgattgcg tcataatgct tttggtaaac cttatcacct tgaataaagg
     9781 acagaaaact ttcttcctgt gctggtgtaa gaggcacctt tggttccgta tcgtcgtcca
     9841 gaacgtcact aagctggaag tcaaacgggt tttttcggat gcaatcgtcc tgtatggcgg
     9901 tgtaaaaagc cgctttcagg gaacgcttgt cgttattgat ggtcttataa gaaaggccct
     9961 tttctttcat ccgaagcgcc cattcctttg cgtcggacag cttcacactg tcaatcgggc
    10021 agctccccag ccgatcttgt tccagaatac gcatcagccg ttcccggccc agtttggtgt
    10081 tgtgcttcac attgccccgg tgcctgatct gcttggcgta gagctggcag acggtcattt
    10141 tcttgccgat ggtgtctatc ccgtcgtcaa ggtctttctg gatttctttc tccttttccc
    10201 ggagtgaaat atcctcccgt ttacctgccg gggtcttatc cgtaggcact aacttccatg
    10261 agtaaacaaa ctgcggtttt ccaaaggtat caacatattt gtaagcatat cgtccgtctt
    10321 ttctctggct ctctccagac cgcaaaacgc gatttttgct gtctcgtctt ttttctgaca
    10381 tctcgtcatg ctcctttcca tgacggaaag agccgcatat atgcttataa attattataa
    10441 catatctgcg gccctttttc actattagat tgtgtccaaa gtgtcaatta ttttctcaaa
    10501 ctgcttgcgt ttgatctgga tgcggttgcc attcataatg acccagccag aggccggatt
    10561 ttcctcggcc agcttacgca atttgttttc tccgatacgg aaatatttag acgcttcctc
    10621 aatcgtcagc gtgtattttt cccaaatagg cacatcatgg ttcataatgc agcccccttt
    10681 cacaaattca aaggtttgtg tgatttaggg gcatagacgg acggctgctg gcacagctcc
    10741 acgggacttt cacccccgcg tggttctcac gcggccctac ccattgcctg cgacgctttt
    10801 aacgctcgga ctgtggctat aagggagtat catagtcctg cctgtacgtc gtcgcccgca
    10861 agccgccacg ctcacattgc ggcgtggtgt tcctcgctcc gcttttcccg tagggaaaag
    10921 tatcatggcg caccagccgt ccggctcggc acagacagaa tgtgtccgta tgccctatga
    10981 tgcgccgccg ttatcttgcg ggcatattta tcaaggtgcg atcaggccac cgggaaaaca
    11041 ccgaaacggg gaaagggtaa gccgtttcat gctgccgggg ccggttcgtc agttcgtgac
    11101 gatagcgtgg atgagcttga tttccagacg gcgcttcatg gcctcgtcca gacacacatg
    11161 cggatagccg ttttcgtcat agagcgtccg ggtacacagc ttgttcatgt aaccgctgta
    11221 atactgcaaa acgcgctcta cggctccggc atccccagcg cgggcggcct cgatcaccga
    11281 aaggggcaaa agctcaatgc ccttgtagtt cgggttcttc atccgcgtct aacctccctt
    11341 cggcatcaac gcctttagct gtttccgcag ctccgataag gtatttgtcc tgtgccgctg
    11401 aacggcactc cgggacattc ccacaaggcc gccgatctca ccatctgcca tatccagaac
    11461 acagtgcagg attaaaatac tctgctccat gctgggcagc gcggcaaagg cttcggcaac
    11521 aagctcattg ttgatatgta ggtcatagcc atgcgacgaa aacgtaaagc tgtcgctggg
    11581 gtagtgatct actgtacaga gcttgtccaa atctgcctgt gacaaagagc ttagtgacac
    11641 ttcacggtca cggcggcgct tcatttcagc caagtagctt ttggcctcgt tacgcagtac
    11701 cgtcttgcaa aaagcatcga acatgcaccg cgcttcatag tcgcggggga tagcatccat
    11761 cttctcaccc cctttctgtg ggggacttag gtggttctct ccctttccgc taacaagact
    11821 ttgacaaggc cgccagctac ccgctaaata cctccttttt gaaaatttct caaaaaattt
    11881 ttttggggac agccgggccg caaaagacga caaaaatcgc ccggcaggtc gcaaacctac
    11941 cgggcgagaa aatgctttgc tatgctgctg atttacatgg tatagtacat actcataacc
    12001 ggaatagccc tgtg
//
LOCUS       DAYYCQ010000085        11733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000085 DAYYCQ010000000
VERSION     DAYYCQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.086"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..155
                     /locus_tag="ACHAYF_002348"
     CDS             <1..155
                     /locus_tag="ACHAYF_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8579763.1"
                     /translation="YSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMPASEAAKKD
                     LIDILR"
     gene            556..1161
                     /gene="fic"
                     /locus_tag="ACHAYF_002349"
     CDS             556..1161
                     /gene="fic"
                     /locus_tag="ACHAYF_002349"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8579764.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1177..1749
                     /locus_tag="ACHAYF_002350"
     CDS             1177..1749
                     /locus_tag="ACHAYF_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8579765.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2001..2258)
                     /locus_tag="ACHAYF_002351"
     CDS             complement(2001..2258)
                     /locus_tag="ACHAYF_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579766.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2384..3199)
                     /locus_tag="ACHAYF_002352"
     CDS             complement(2384..3199)
                     /locus_tag="ACHAYF_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8579767.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3716..5041
                     /locus_tag="ACHAYF_002353"
     CDS             3716..5041
                     /locus_tag="ACHAYF_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8579768.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5034..5384
                     /locus_tag="ACHAYF_002354"
     CDS             5034..5384
                     /locus_tag="ACHAYF_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579769.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5366..5551
                     /locus_tag="ACHAYF_002355"
     CDS             5366..5551
                     /locus_tag="ACHAYF_002355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579770.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6455..6730)
                     /gene="prgO"
                     /locus_tag="ACHAYF_002356"
     CDS             complement(6455..6730)
                     /gene="prgO"
                     /locus_tag="ACHAYF_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8579771.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6702..7655)
                     /locus_tag="ACHAYF_002357"
     CDS             complement(6702..7655)
                     /locus_tag="ACHAYF_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8579772.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8267..9760
                     /locus_tag="ACHAYF_002358"
     CDS             8267..9760
                     /locus_tag="ACHAYF_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8579773.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9895..10191
                     /locus_tag="ACHAYF_002359"
     CDS             9895..10191
                     /locus_tag="ACHAYF_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8579774.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10292..11143
                     /locus_tag="ACHAYF_002360"
     CDS             10292..11143
                     /locus_tag="ACHAYF_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579775.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11164..11376
                     /locus_tag="ACHAYF_002361"
     CDS             11164..11376
                     /locus_tag="ACHAYF_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579776.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4037 a         1809 c         2173 g         3714 t
ORIGIN      
        1 cttatagtgt tggttctttg ataaaggtaa acgtgtcact tgatctattt actattttac
       61 tagcggttgg ggtatcttcg gttattggac tggtcttttc tgtaatgccc gcttcagagg
      121 ctgcaaaaaa agatttaatt gatattctta gataaaatca gaatgacaag aatatttacg
      181 ttctagattc taaaataaaa cacaaagaga ccaaccaggc accctattgt ttctttgtag
      241 tatagagata ttctttggct tgtaagagat caatagagtt aggtgaccgg agttcgaaat
      301 aaaggaaatt tttgattgta taggagaatt ctttatccct aatatttaga attttgagta
      361 tgccatgttg atgttttaga ttctctataa atatagaggt gtatcaaagg tcaactagac
      421 tgtgcaaaat agagacactt gaaaaatcta tcgggggtca gaaacttaat agtttttgac
      481 acataaacaa aatcgcgata taatggggtt gagagtgttt cataaactta tgtcataaag
      541 ttaaggagat ttcgcatgat tttagagaat aaattgggat taaccaatca agttgagttg
      601 gctaaggttg aagaaaaact cagtaaacaa aaagcgaaag aattgtacga ttgtggaaaa
      661 ataaatgaaa tcgaagttgg tacatttaaa ggtttatctg aaattcatga atttctattt
      721 tcggatattt acgattttgc cggaaaaatc cgatcggtca atattgcaaa aggaaatttt
      781 cgatttgcac cagttatgta tttagaacat tcgttacagc atattgatca gatgccacaa
      841 acttcttttg aagagattat caagaaatat gtggagatga atatcgctca cccatttcga
      901 gaaggaaatg gccgaagtac tagaatttgg ctggatctca ttttaaagga agaattgcaa
      961 aaagttgtag attggaactt aatcgataaa gcagactatc tttctgcaat ggagcgaagt
     1021 cccataaacg atttggaaat acgctatcta atttccaatg ctctgactga taaaattgac
     1081 gatcgcgagt tatatatgaa aggaatcgat gtcagctatt tctatgaagg gtattctgag
     1141 tatacgatag atgatttgta aaagtaggtg gaccaaatga cgaaatatgg atatgcacgt
     1201 gtctcaaccg cttctcaaag tacaaaagaa caaattcaac agttgcttga gaacggcgta
     1261 gagaagaaga atattttttc agaaaaattt acgggtacaa aacgggatcg gccaaaattc
     1321 aatcagctgt tggctggtct gaagcctgga gatgaaatag ttgttacgaa gcttgatcga
     1381 tttgctcgta acacgagaga agcattagag atcattgaac ctttactcga tgataacgta
     1441 acgatcaagg ttttgaatct aggaacgatt gaaaatacgt ccatggggcg aatggtgaca
     1501 cggacgttgt tgagtgtagc agaaatggaa cgtgatatga ttgtggaacg tacccaagaa
     1561 ggaaaaatgt ttgcaagaaa gaataatcct aatttcagag aaggacgtcc gaaagcaaca
     1621 atcacaccga agaagaggca cgcatatgaa ctgcttactt caggtaaatc gtataaggaa
     1681 gttgaagcta taaccggttt cagtcgcagt acgcttttta gaataaagaa gaaaattgaa
     1741 gaaagttaaa aaagaagtct cagcgaacca gacaggtaaa tacttaatgc gttctagata
     1801 tgagtctatt agcatgttgt gaaatataat atttttttat ttacttgatt tctgctcaaa
     1861 aaatgtgtat ggtaactgta aaaggtaaag taattaaagg tatgctagcc acgcttacta
     1921 tattaggata ccatcatata tttataatga acgatttgtt cgtctaccaa acaacacata
     1981 tcgttcagcg taagtcacgt ttaggaacgt ggctttttta gtgttattat cgataaataa
     2041 tctattgact ctattgattt tatctccata tccctacggt gttgattgtc tttagatctt
     2101 tcatttaaga accaaataac tatcataaaa atcagcactg ctgctaatat aataaacagc
     2161 attcctattg tgatattacc tattgatttt accataacta cgattaaagt aataatggca
     2221 ttcaagtctc ctttgttctc tatatataaa ttagccataa atcatcttcc tttcaaatta
     2281 agatacttgt ttgataccaa taaatagtaa gcgtggctag catacctttt tctgtaatag
     2341 caagtgttct aatagtccaa acttaatgat ggaccgatgc tagttatagt acagtaatgt
     2401 tttctttctt atctagattt ttagtaagct ctatcagcaa atctaacaaa tctgagtcct
     2461 tttcagaaac taaattaaat ctagtgttta caacaaagtt ttcatgttct atattgttta
     2521 gattatttgc ggaaaggagg gcttctagat cttgacattc aaatctatat gcatcgtcat
     2581 aaatcttact agaaggagtg cttaattcga aattacttat tttctcatct tggaccaaga
     2641 taaatttata gatagtatct ttttgtgttt ttcttttaaa aacacaacag agtgtgttat
     2701 aaggaacgtt ataattttca tcagcctcat tttttctcca ttcaaacaaa tgataaactg
     2761 caaccaagtt gccatcaatt tcatggacag tgctctcggt aacatccttt aaagaactat
     2821 caagggctaa tttatagtaa ctagagagag gaagtaaaat tgtcgattta ctgagatggt
     2881 tatcgtctat acgtgcaatc atacctaaat caactaaggt tttattccag gtgtttatag
     2941 ttttagttgt cactcctaga gactctgcta tggtattgac cgaatgccag gaatccccgc
     3001 cttcattctt tgccttaaaa cagtaataca gatataaatt aatagctttg ttattcggta
     3061 aatatttttc aagttctgtt ggtagaataa agtaagaatc cttaatgttt tttctgaact
     3121 gtctccagtc tctgtagttt tgtttataaa gttgtgaaac ggtttttcca cctttagaaa
     3181 gtcctgctat cttaaccaat cttattgctc ctttcttgaa tctgtcatga tcatacagta
     3241 ttttttactt gtagtccagc accttaatat attatcctat gtaattaatt tcgtactaga
     3301 tagtaattat tttttattta attctctagt cacttacaaa taagttatat aaactacgat
     3361 taataaatta ttacaaaaac tattaagaaa acttctacat acaactatta caaaactatt
     3421 aagaaactga atactcctat tttttgtttt taaggaaagt tttttctttt ttgaatttct
     3481 tatttttttc taatataact aagcaaaact ggataatacg acacttattt tcaaattttc
     3541 acaagactaa tagtaaatat ccatttaata aagtgataca atgtgttgat tttacaaatc
     3601 aacagaagaa gtagggaaat aacttaccta tgcttctttt tttgtacatt taatcgattc
     3661 ttctttacaa aaacgaacgt atgtttgtat aatgtggaag aatggagtgt tatttatgat
     3721 ggattattca aaagagccag taaatgatta ctttttaatc gatatgaaaa gcttttacgc
     3781 aagtgtcgaa tgtatagaaa ggaatttaga tccattaacg actgaattag tcgtcatgag
     3841 tcgagcggat aatacaggat cagggttgat tttagcttct tcaccagctg caaagaagaa
     3901 gtacggtatt tctaatgtca gtcgaccacg agatctaccg caaccttttc ctaaaacgtt
     3961 acatgtcgtt ccaccacgca tgaatctcta tattaagcga aatatgcagg taaacaatat
     4021 tttcagaagg tatgtagcag acgaagattt attgatttac tcgatcgatg aatcgatctt
     4081 aaaagtgacc agatcaatga atctttttac gactgaagga acaagaagtc aacggagaaa
     4141 gaagctggct caaatgatcc aggagaatat taaagaagag ctaggattgc ttgctacagt
     4201 aggtgtcgga gataatccct tattagctaa acttgcttta gacaatgaag caaaacataa
     4261 tgatggattc cttgctgaat ggacgtatca aaatgttcct gataaagtat ggaatattcc
     4321 tgaaatgaca gacctttggg gtattggatc tcgaatgaaa aaaagactca accaaattgg
     4381 tatcatgagt attaaagact tagctaattg gaatccatac actattaaga gccatttagg
     4441 tgtgattggc ttacagctgt attttcatgc gaacgggatc gatcgaacag atatagcgat
     4501 cccaccagaa ccgacaaagg aaaaatcata tggtaattca caagtgttac cacgagatta
     4561 taccaggaga aatgaaatcg aactggtggt aaaagaaatg gcggaacaag tagctattcg
     4621 tatcaggcaa cataactgta aaacaggttg tgtccattta tacattggca cgtcgatctt
     4681 agaaacgaga ccaggttttt cacaccagat gaagatccct ataacagata atacaaagga
     4741 attgcagaat tactgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca
     4801 cgttggaata acctatagca agcttgtgta cactgattcg cttcagctgg atttatttag
     4861 tgatccacaa aagcaaattg acgaagaaaa tttagataaa attattgaca agattcggca
     4921 aaaatatggt tttacgtcga ttgtacatgc ttcttctatg ttagaaggcg ctcgtagtat
     4981 tactagaagt acattggttg gtggccatgc aggcggcgca ggagggctta aaaatgattg
     5041 attataatga tcgtggacgt ttaaagtggg caggttttta tttatctgat catactgaaa
     5101 aaattgatgc cgacaatgaa caacgagcca atcaaaattt ggcaaaagaa caaatgacga
     5161 ctgaagaggt tgcggaagta ttgaacgctg cagtcataaa aaatagatca gtatgtattc
     5221 aaaaagaaga acgaaacgct gaaggatact atccagaaga tattgttagc aagatcttag
     5281 gtagtgatga gctggggatc tacgttggaa aaaacaaagt tcactacgac gaaatacgtc
     5341 atatcgaatt ttaccaggat aataaatggg gtcgattaga atgaacatta tccagcaata
     5401 tgagctaaag tatataacct ttgatcagct atcagaagag atttggggtt atggccagcg
     5461 tttgataaat gaggttggtg ttgaacgctt ctctttctat gtagaagctg cagcaggata
     5521 ccataaatac gaatactata ttggctatta aagtgcaata aaattatatt tcttttatgt
     5581 tttacatttt gtatcaattc tgataaaatt acctataggt aattaaaaaa tataaaaaaa
     5641 ctagttacaa ccttaggacg ttgtaactag cttgaagcat actagagcta actaaaatag
     5701 cactagaaag caatgttaaa cccgtccaac aaacttgatt taacattgag accagcttat
     5761 tcagctaaat atagttgaat aagcttcttt tgttttaaaa aaacaaaagg tgtactacga
     5821 ataacgcaag taaaagaaat actattacaa ccagtaggct acaagtaagt attactaaac
     5881 attgcgtagt tagaaccttt gctctattgt ctaaagccac aaaattagag atcagcttta
     5941 tgatgctaag agcgagctgc atccatgcgg acataggttc ttttgacact attaattctc
     6001 ctttttagga taagggaaat tgctagtagt tttttcatta gcaccatccc ccttcatttg
     6061 attgagtcta gtgctgctgt gatagtttgc tgtttaatat gcttacattc ttattataac
     6121 ataaatgaaa caaacacatg gttagaggtc gtatcaatca aaatagtgtc tcacaaacga
     6181 tttattactt actcctgctg actaacggac tgttcttcag taactgtaac tatatgtttg
     6241 ttaaaaccac cattacctgt agataaaaac caatatattt actatactca ctatattaaa
     6301 tatatttagt atagtaaaaa ctagaaacaa taaaatttgt gaatgttgta tacccccaaa
     6361 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
     6421 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     6481 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     6541 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     6601 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     6661 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     6721 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     6781 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     6841 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     6901 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     6961 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     7021 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     7081 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     7141 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     7201 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     7261 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     7321 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     7381 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     7441 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     7501 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     7561 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     7621 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     7681 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     7741 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     7801 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     7861 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     7921 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     7981 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     8041 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     8101 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     8161 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     8221 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     8281 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     8341 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     8401 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     8461 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     8521 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     8581 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     8641 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     8701 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     8761 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     8821 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     8881 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     8941 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     9001 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     9061 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     9121 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     9181 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     9241 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     9301 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     9361 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     9421 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     9481 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     9541 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     9601 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     9661 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     9721 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     9781 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     9841 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     9901 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     9961 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
    10021 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
    10081 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
    10141 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
    10201 tttacttaaa aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg
    10261 ttaaaggaaa gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacac
    10321 ctggcttcct ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc
    10381 attaggaaaa ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat
    10441 tggagtattt ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga
    10501 catttttaca gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt
    10561 agtcatgcaa tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga
    10621 tttaattgtt gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag
    10681 aacaacaaaa ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat
    10741 gtatttgttt gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat
    10801 taaacgttca attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga
    10861 gtacaatgtt gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag
    10921 tatccatgaa tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa
    10981 agcaatagat cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata
    11041 tggcatcgga aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt
    11101 ttggaacagt agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg
    11161 aaaatgattc ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag
    11221 aaggagcagt tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt
    11281 acgatgaaca ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg
    11341 ttgattcgaa agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg
    11401 atgaatggga acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg
    11461 taaagagcat aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc
    11521 tattacgttt ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg
    11581 atagtgaagg atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc
    11641 tatccttttt aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa
    11701 atcgtaagag aaattgatcg aagatgtgac gaa
//
LOCUS       DAYYCQ010000086        11642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000086 DAYYCQ010000000
VERSION     DAYYCQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.087"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1055
                     /locus_tag="ACHAYF_002362"
     CDS             <1..1055
                     /locus_tag="ACHAYF_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8579777.1"
                     /translation="KKRKVSVTLSSNSRQAWNQANLLLNEKIAEKIKRNEELPLTFGE
                     LKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDDVLVKNVNSNLIQDMLDYYYFEKN
                     LSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEE
                     VQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELK
                     IKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQ
                     SLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTT
                     KNMQNQLVEKLGKIEI"
     gene            complement(1137..2909)
                     /locus_tag="ACHAYF_002363"
     CDS             complement(1137..2909)
                     /locus_tag="ACHAYF_002363"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579778.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(2912..4624)
                     /locus_tag="ACHAYF_002364"
     CDS             complement(2912..4624)
                     /locus_tag="ACHAYF_002364"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579779.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4739..5407
                     /locus_tag="ACHAYF_002365"
     CDS             4739..5407
                     /locus_tag="ACHAYF_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8579780.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5474..6262)
                     /locus_tag="ACHAYF_002366"
     CDS             complement(5474..6262)
                     /locus_tag="ACHAYF_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8579781.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6345..7817)
                     /locus_tag="ACHAYF_002367"
     CDS             complement(6345..7817)
                     /locus_tag="ACHAYF_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8579782.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(7947..9140)
                     /gene="metK"
                     /locus_tag="ACHAYF_002368"
     CDS             complement(7947..9140)
                     /gene="metK"
                     /locus_tag="ACHAYF_002368"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8579783.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(9138..9217)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(9406..9477)
                     /locus_tag="ACHAYF_002369"
     tRNA            complement(9406..9477)
                     /locus_tag="ACHAYF_002369"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9442..9444),aa:Glu,seq:ttc)
     gene            complement(9489..9562)
                     /locus_tag="ACHAYF_002370"
     tRNA            complement(9489..9562)
                     /locus_tag="ACHAYF_002370"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9526..9528),aa:Ile,seq:gat)
     gene            complement(9575..9645)
                     /locus_tag="ACHAYF_002371"
     tRNA            complement(9575..9645)
                     /locus_tag="ACHAYF_002371"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9611..9613),aa:Gly,seq:tcc)
     gene            complement(9650..9722)
                     /locus_tag="ACHAYF_002372"
     tRNA            complement(9650..9722)
                     /locus_tag="ACHAYF_002372"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9687..9689),aa:Phe,seq:gaa)
     gene            complement(9726..9798)
                     /locus_tag="ACHAYF_002373"
     tRNA            complement(9726..9798)
                     /locus_tag="ACHAYF_002373"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9763..9765),aa:Asp,seq:gtc)
     gene            complement(9817..9890)
                     /locus_tag="ACHAYF_002374"
     tRNA            complement(9817..9890)
                     /locus_tag="ACHAYF_002374"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9854..9856),aa:Met,seq:cat)
     gene            complement(9903..9992)
                     /locus_tag="ACHAYF_002375"
     tRNA            complement(9903..9992)
                     /locus_tag="ACHAYF_002375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9954..9956),aa:Ser,seq:tga)
     gene            complement(10015..10086)
                     /locus_tag="ACHAYF_002376"
     tRNA            complement(10015..10086)
                     /locus_tag="ACHAYF_002376"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10051..10053),aa:Glu,seq:ttc)
     gene            complement(10108..10195)
                     /locus_tag="ACHAYF_002377"
     tRNA            complement(10108..10195)
                     /locus_tag="ACHAYF_002377"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10159..10161),aa:Ser,seq:gct)
     gene            complement(10220..10293)
                     /locus_tag="ACHAYF_002378"
     tRNA            complement(10220..10293)
                     /locus_tag="ACHAYF_002378"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10257..10259),aa:Ile,seq:gat)
     gene            complement(10307..10377)
                     /locus_tag="ACHAYF_002379"
     tRNA            complement(10307..10377)
                     /locus_tag="ACHAYF_002379"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10343..10345),aa:Gly,seq:tcc)
     gene            complement(10379..10454)
                     /locus_tag="ACHAYF_002380"
     tRNA            complement(10379..10454)
                     /locus_tag="ACHAYF_002380"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10419..10421),aa:Asp,seq:gtc)
     gene            complement(10473..10546)
                     /locus_tag="ACHAYF_002381"
     tRNA            complement(10473..10546)
                     /locus_tag="ACHAYF_002381"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10510..10512),aa:Met,seq:cat)
     gene            complement(10559..10648)
                     /locus_tag="ACHAYF_002382"
     tRNA            complement(10559..10648)
                     /locus_tag="ACHAYF_002382"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10610..10612),aa:Ser,seq:tga)
     gene            complement(10672..10745)
                     /locus_tag="ACHAYF_002383"
     tRNA            complement(10672..10745)
                     /locus_tag="ACHAYF_002383"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10709..10711),aa:Ile,seq:cat)
     gene            complement(10759..10832)
                     /locus_tag="ACHAYF_002384"
     tRNA            complement(10759..10832)
                     /locus_tag="ACHAYF_002384"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10796..10798),aa:Met,seq:cat)
     gene            complement(10860..10932)
                     /locus_tag="ACHAYF_002385"
     tRNA            complement(10860..10932)
                     /locus_tag="ACHAYF_002385"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10897..10899),aa:Ala,seq:tgc)
     gene            complement(10952..11025)
                     /locus_tag="ACHAYF_002386"
     tRNA            complement(10952..11025)
                     /locus_tag="ACHAYF_002386"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10989..10991),aa:Pro,seq:tgg)
     gene            complement(11035..11108)
                     /locus_tag="ACHAYF_002387"
     tRNA            complement(11035..11108)
                     /locus_tag="ACHAYF_002387"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11072..11074),aa:Arg,seq:acg)
     gene            complement(11119..11204)
                     /locus_tag="ACHAYF_002388"
     tRNA            complement(11119..11204)
                     /locus_tag="ACHAYF_002388"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11168..11170),aa:Leu,seq:taa)
     gene            complement(11221..11292)
                     /locus_tag="ACHAYF_002389"
     tRNA            complement(11221..11292)
                     /locus_tag="ACHAYF_002389"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11258..11260),aa:Gly,seq:gcc)
     gene            complement(11304..11376)
                     /locus_tag="ACHAYF_002390"
     tRNA            complement(11304..11376)
                     /locus_tag="ACHAYF_002390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11341..11343),aa:Thr,seq:tgt)
     gene            complement(11394..11475)
                     /locus_tag="ACHAYF_002391"
     tRNA            complement(11394..11475)
                     /locus_tag="ACHAYF_002391"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11439..11441),aa:Leu,seq:tag)
     gene            complement(11483..11555)
                     /locus_tag="ACHAYF_002392"
     tRNA            complement(11483..11555)
                     /locus_tag="ACHAYF_002392"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11520..11522),aa:Lys,seq:ttt)
     gene            complement(11559..11631)
                     /locus_tag="ACHAYF_002393"
     tRNA            complement(11559..11631)
                     /locus_tag="ACHAYF_002393"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11596..11598),aa:Val,seq:tac)
BASE COUNT         3623 a         2600 c         2067 g         3352 t
ORIGIN      
        1 aaaaaaagag aaaagtgtcc gttactttat ctagcaattc acgccaagct tggaatcaag
       61 ctaacttact tttgaatgaa aaaatcgcag aaaaaataaa acgaaatgaa gaattaccac
      121 taacttttgg agaattaaaa actaaatggg atgaaaaata caagccaact gtaaaagaaa
      181 gttcctatcg aacaactcag gtttatctat cattaatttc aaaatatata aaagatgatg
      241 tacttgtaaa aaacgttaat agtaatctta tacaagatat gctagattac tactattttg
      301 aaaaaaattt atcatataat tatgtaatac atttaaaaac ttttacagga atgattttta
      361 aattcgccaa ccgtcgatat ggattaaaat ataatcccgt aaatggggtt tctttagcaa
      421 gaaaacctaa gacaaacgaa gaaataaaaa aagaaaaaga tggttattta tcaaaagaag
      481 aagttcaaat gattgctagt aaacaaaaaa gttcacatca acagtcacgt tattctttaa
      541 ttactaaatt tttgttttta actggactac gttatggtga attgatatct ttgactgaat
      601 ctgattatga tggtaataaa attacggttt ctggtactta tgattacgag ttaaaaatta
      661 aaacaacaac taaaaataca ggttcttata gaaccattga attatcaagt aatgctaaag
      721 aaatcataga tcaattaatt gaagaaaaca aattaattaa gaataataaa gataaataca
      781 ttttcatctc taaaaatggt aacccgatat ctattcaagc atataatcaa tctttaagag
      841 cagtatccaa agaattaaac ctagataaaa aagtttcttc tcacatgtta aggcattccc
      901 acatttctct acttacagaa ttaggaatcc cattaaaagc cattatggat cgtgtaggac
      961 atgaagattc aaaaacaaca ttaaagattt atactcatac aactaaaaat atgcaaaatc
     1021 aactagtaga aaaactagga aaaatagaaa tttaaattgc cccttttctg ccccttataa
     1081 aaacaaacaa cataaaatat ataaaaagaa ccccgttata acgaggttct tttaatttaa
     1141 ctttcaaata caaattgatt atgataaagt tccgcataaa atccattttc tgctaataac
     1201 tcgtgatgac tgccttcttc aatgacttcc ccatcacgca agacaacgat tctatctgca
     1261 ttcaagattg ttttcagccg gtgagcaatc acaaagctgg ttcttccttt gattgcttca
     1321 tccattgctt tttggatcct tgcttctgtt accgtgtcta cattacttgt tgcttcatcc
     1381 aaaatcaata aagatggatt tgtgatgatc gttcgagcaa tactgatcaa ctgcttttga
     1441 cctgtactga ataagttatt ttcttcggat atctctgtat cataaccttt ttctaacgtc
     1501 atgatgaagt catgaatatt cgcttgtttt gctgcattga tgacttcttc atctgaagcg
     1561 tccggtttgc cgaatgtgat attgtctttg atcgttccgg agaacaatac agattcttgc
     1621 aaaacgattc ctacatgtct acgtaatgca tcaagatcat aatctcggat atctattcca
     1681 tcgatgacta ctgaaccatc attgacatcg taaaaacggt ttagcagatt catgatcgtc
     1741 gttttccccg atcctgttgg accaacaaga gcaaccattt ctcctttgtc cacattgatc
     1801 gagacatctt tcaagactag tttttctgga gaataaccaa aatctacgtg ctgcaacgca
     1861 agaccttttt caacaccatc aaatgcttta cagttttctg gtttgacttc atctttttca
     1921 tcaaatattt cattcaatcg tcttgcccct gtaatcgcta attggatcat gctgtatcca
     1981 gatgaaatct gcatcagtgg ttggtaatat tgttgtgaat actggacgaa tgtcacaact
     2041 aagcctaatg ccaccgaacg ttccaaatca ccatttaatg ctaaccagcc gccgaaaaag
     2101 ataactatcg ctgtattgac cagtgacatt ccttgcatca tcggaaatag tagaccagaa
     2161 taagcttgtc ctttgaaggt tgcttcgcga acactttgat tatgctctaa gaatccttca
     2221 atcgtttctt cttgtaagcc atttgtgatg atgacacgtt gtccgctgat tttttcatcc
     2281 atataaccat ttagtttccc cacttcatct tgctgaagat ccacatattt ccgtgctttg
     2341 cggataatca agacagctat caaaacggct actggagtgg aagcaattgt agcccaagct
     2401 aactctacat tttggcggaa catcatgatc aagatcccga caagtaaaac agcattcgtg
     2461 atgacttgaa gcaaagcttg gttcaaacta ttttgaatat tatctaagtc actggtaaaa
     2521 cgacttaaaa tttcaccgtc ttgatgagaa tcaaagaaac ggatcgtcaa tttttccaat
     2581 ttgttgaata gaccgatacg cattcggttc gttgacttcc ctactacttg tgtgaacaaa
     2641 atactataga taaagttggc tgcactagct agtacataaa acagcaagag tttccatatt
     2701 acagaaacaa atttgctttt atcgtctacg ccttgcatca agcctcctac gtaattagct
     2761 aattcttgaa aagcttcccc gatgtattga ggtgccttta cttgtagata ggtagcaatg
     2821 atcactgtga taaagataaa gaaaaaggag agtttgtacc gcttcaaata gtgatagaaa
     2881 aatttacttg cttttattag atcagtcata cttactcctc cgttcctttt tgagtttcaa
     2941 agatttcctg atagacacgg tttccagcta ccaattcctc atgggttcct tcgccgacta
     3001 atctgccttc atccaatact aagatgcggt ctgctttgac aacagaagca attttttgtg
     3061 cgatgacaat cgtcgttgtt cccttcaaat ctcgatcgag tgcttctcgg accagctttt
     3121 cagagcgagc atccagtgca ctcgtactgt catctaggat caagatcttt ggattaccga
     3181 taacgccacg agtaatggat aaacgttgct tttgaccacc agaaaagtta ctgctacgtt
     3241 cttctattgg tgcttcgtaa cgatcagata acttttcgat gaattctttt gcttgagcaa
     3301 tagaagaagc atgttccatc tctttttctg ttgcattctt tttcccttgg cgcaagtttt
     3361 gagcaatcgt tcctgagaat aaaattgctt tttgcaaaac aaaggaaacc gtttgtctta
     3421 aactcttttc atttacttga cgaagatcta caccacctac ttcaactttg ccttctgtcg
     3481 gatcaaatag acgagggatc aattgtgcta gtgttgactt cccggcacca gtcgccccga
     3541 cgataccaac catttctcca ggtttgatag aaaagctgat atctttaagc gtatctgtat
     3601 cctcaccagg ataacgaaaa gaaacatggt caaaagtgac agaacccgtc aactcttgtt
     3661 ctggaacgtc taaataagta agatctggtt cagcatctaa aatttccgca atccgcttca
     3721 gcgatacggc tgctcttgaa gtcatcatca tcatcattcc gccaataatg attgccatca
     3781 taatctgcat caagtagttc atgaatgaag caattccacc aattaacgta ggatcatctt
     3841 taacaagatc gctcacaaaa aagatcgccc caaccacagc caaattggca gccagcatga
     3901 aggacgggat catcacagaa aataatgtac cgactatgat gttgtgtttt gttaagtcct
     3961 cacttacttt gctgaatgac ttcaattggt tttcttcttg tacaaaagat ttcactacac
     4021 gaatccctag taagttttct tttgcaatgc tattcacacg atcaatcagt ttttgaatga
     4081 tcatgaagtg cttgcccatc cttgtaaatg aaagtgccgt aatcagaaat accgtaacga
     4141 taagcgctac gatgatccac cataattgcg gcatcgtgat catagctaaa atgaaggcac
     4201 caataaataa gaatggaatt cgaaaaagtg attgcaaaga aatcatcact aaattttgga
     4261 tttgcgtgat atcatttgtt agacgaacaa caagatttcc agcagagaac tgttcgatat
     4321 ttccaaatga aaatgtctgg atctttcgaa atgcttcttc tcggatatct gcacttacgc
     4381 cttgagctac ttttgcagag aaaatcgtgt tcgttacacc tgcggctaat cctagcaaag
     4441 ccagactgat caggtaaata ccaattgttt tcatttcatc attatcttct gtaataatag
     4501 cttccaatac ttgctgtagt aacttaggtt gccataatgc tgctgcaacc atgactgcta
     4561 cggacagcaa ggaaataaaa acagctactt tatatttttt cgcatgtcgt aagattaatt
     4621 tcattcgttg attacacccc tttgtttaaa ttgacaggac taatttaaca ctgtaaaatt
     4681 agaccgacta acaatttacc atgtttaaaa agataatgca atacacgagg ttgataaaat
     4741 ggaaataaag ctttctcaag caatcatcat tcaggccgcc tttgatatcc tggctgaaac
     4801 acaagctctt tcgcagcttt ccatgcgaaa catcgcaaac agaattcatg ttcaagcacc
     4861 tgctttatat tggtatttca aaaacaaaca acagctcttg caaataatgg ctgaaacaat
     4921 ggaagaagaa ttagcgcttc cagatccaac tttgccttgg aatgaacagt tgctgcagta
     4981 tatggaaaat tactatgacc tttatacacg tttcccttgt ggggcagaat tagaaatcaa
     5041 tactgtgccc gcttttccga gtcgattaga acatttggag caaatgatcc aattacttgt
     5101 tcgtgaagga ttttcagtca cccaaagtca tcaggccatt gcctctctcc acaatttatt
     5161 gattggacag ttaatggatc agcagcatga acagcagctt cgacaaaaaa ttatggacgg
     5221 aaatgaattt ttgaaagaaa cagttctatt tatgcgtaat tatgtagttg aaaatcaatt
     5281 agagggattt aaagaaagta tgcagtccca tgcagaattg aatgaaaaag cactctttct
     5341 aacgagtgca caaacctata ttgaaggctt atgccaacaa aataaaagaa gagaaaacga
     5401 gcagtaaaaa agctcctata gagatcacta tgtttgtgat ctctatagga gctatctttt
     5461 taagactctt tttttatttt tcttccatta gccacgccaa tgcacgttga atccattggt
     5521 cccagaagat ccagttatga tcgccaggtc cttgttcaaa ggtgaccgcc aagccttgtt
     5581 cctgcatagc agataccatt tcctgatttg cctgataaag aaaatcagat tccccacaag
     5641 ccatataaaa tcttggaact ggttttccgc tttttactag ttctttcaat aagtattgcg
     5701 gatcattttt tgaaccttcg atttgatcca atggtccaaa gatcccttcc caaaaagatg
     5761 ctttacgaac catcaaaaga tcatccattc gatttcctac agaaacagcg cctgaaagag
     5821 agattactgc tgcgaaattt tctgattttg caaggcctag ttttaacgcg ccatatccgc
     5881 ccatcgataa tccagcggca tatgtttttt ctctttttct tgttaattgc gggaataatt
     5941 catgacaaat ctcaggtagc tcttctgcaa tgaatgtcca atacttcatg tcataaactg
     6001 tatctgtgta ccagccaaga tcagtagatg gcatgatgac agctaatccg tagtttgcta
     6061 cataacgttc aatcgacgta cgtctttccc atacactatg attgccaccc attccatgaa
     6121 gtaaatataa aacaggtata tccgttgtct cttgctctga actggtgcca atcacctttt
     6181 ttgttgtttg cggtagtatc acatttagat tgacttccat ttctaacaca ttcgaataaa
     6241 tatttgcttg aataaatgcc atattttcgc cttccttagt aagaagttat ttttgaataa
     6301 ctttaaattt gaatgattcg gctacgtaca tatcttaacc gaaactactg ttttatttta
     6361 ccactttttt catgaacgaa aagcgttaag accaaagcaa tgatcacgag taacaagcca
     6421 actagataaa cggaatgtaa cccatcataa agaatttttc ttaatggtaa caaaatatcc
     6481 gcatcaatat atttagccgt ttgtggattc accagtttat tcatgatatc tgcatcattt
     6541 gttaaatttg ctgctgcaag ttctcgttta gttaccccat ttatcaataa accaaaaata
     6601 gaaaccatga ctgtttgacc aattgttcga actaacgtat taaaagaagt tgctacacca
     6661 agctggtcac gagatacact attttgtgca ctgacagtcg aggtagtcat ggtcacacct
     6721 aagcctatac cgatgaccgt tgatatagca aaaaaccata aatagttact tctgaaaggc
     6781 ataagataca accaagcgcc cccaatagct gcgattagta gtccaattct caagacccaa
     6841 tgagttttca tttgagtcat ccacttgctt gcaaggaaag aaccgaccat ccagactagt
     6901 gataatggtg ctaataccag cccaccaatt gctgcattca gccctaatac accttgcatc
     6961 cacataggga tataaacatc gattcccatc aaaaaaccac tagccaatgc tgcagccaag
     7021 ttgaccacaa caaatagtgg atttttgaat aaatgcatca tgatgacagg atctttagct
     7081 cttttttcgg caaaaacaaa aatacctaac attacgatac tacctagcag caaagcagaa
     7141 acaagcggac tcattccttc gtctcctaat aattggaacc ctaacagcaa tgagacaagc
     7201 atggccatca aactaaaact accaaaaata tccattggtt tagactcatg tatgattttt
     7261 ggttcattta aatatagcca aatcaaaata attaatagaa ttccgatcgg cacattgata
     7321 aaaaagatcc agtgccagct gatcgtttca acgatgaatc ctcccgcaag cggtccaaaa
     7381 acactggcaa tcccccaggc tgaactattc aaacctaaaa cttttgctcg cttttcaata
     7441 gaataaagat cgccgatgat cgtcaaagct actggcatca ttgccccagc acccatccct
     7501 tgaatcgcac gagcagcaat caacccaatc atgctttgag ataaaccgca aaataacgaa
     7561 ccgataataa acaataatgt cccaaaaata aagattggtt tccgtcccac tttatccgca
     7621 agttttccgt aaatcggagt aagcatagcg tttgtcagca aataaatcga aaatacccag
     7681 ttcatgattt ccattccatg taatgaaccg acaatcgttg gcatagcggt tgttacgatt
     7741 gtaccttcaa tagccgtcat gaacgttgca atgaaaatac tcactgtgac caaagtaaca
     7801 tttgtttctt ttttcatctt attcctccct ttcattatga caggatctaa aaatagataa
     7861 ataattacta tctttttcaa gaggttaact gccataaaaa aagctggaac aaccaccact
     7921 tggcgatcgt tccagctgaa tataacttat tttgctaaac ttgctttcaa cgcatctact
     7981 ttgtccgtat gttcccaagg caaatctaca tctgtacgac caaaatgacc gtaagcagct
     8041 gtttgtttgt aaatcggacg acgcaaatca agcatctcga tgattcctgc tggccgaaga
     8101 tcgaagtttt ctctcactgc agcaatcagt tcttcttccg gaaccgttcc agtaccgaaa
     8161 gtattgattg agatcgaaac aggttgagca acaccaatcg cataagctag ctgtacttct
     8221 gctttgcgag caagccctgc tgcaacaatg tttttagcaa tataacgtgc agcatagctg
     8281 gcagaacggt caacttttgt cgcatcttta ccagaaaaag caccgccacc atgacgagca
     8341 taaccgccat acgtatcaac aatgattttt cttcctgtta atccggcatc cccttgagga
     8401 ccaccaatga caaatcggcc agtcggattg atataatatt tcgtttgatc atctaataat
     8461 tcagctggaa taacttcctt cactactttt tcgatgacat catgacgaat tgtttcatta
     8521 tctactgcat catcatgctg tgttgaaata acgattgtat ctacgcgttc cggttgtcct
     8581 tgatcatcat attcaaccgt tacttgagat tttgcatctg gacgtaaata cgttaattca
     8641 tttgatttac gcaaatctgc caagcgtcgt accagacgat gactcaaagc gattggtaaa
     8701 ggcatcaatt ctggtgtttc atctacagca aacccaaaca ttaaaccttg gtcaccagca
     8761 ccaatctcat ctaatacgtc ttttttatct tcatcccgga tctcaagtgc ttcatcaact
     8821 ccttgagcaa tatcaggaga ttgttcatca atcgcaacca atacagctgc tgtatctcca
     8881 tcaaatccga attttgcacg tgtataacca atttctttta ttgtttcccg tacgactttt
     8941 tgaatatcta catatgccgt cgtagatatt tctccaaata ctaaaacaag accagttgtt
     9001 acagacgttt cacacgctac tcgtgctgtt ggatcttgtt ttaaaattgc atccaagatt
     9061 gcatcactga tttggtcagc aattttatct ggatgtcctt cagaaacaga ttctgatgta
     9121 aataagtgtc tttctaccat tataatgatt ccccctagtt aaattcggtt acaaggcatc
     9181 tccgtcctgc ggatcctttc gggaacttgc aacgaaccaa gtatagcaga taaaacagtt
     9241 ttctgctcgt attttttcat ttttttgctt aaaaaatata aaaatatttt acattagaac
     9301 tgtccaaaaa agcttagcac aatcatccat aaagataact ctgctaagct ttttaaaatc
     9361 attctttcag ataaaaagaa aaagatactc aaaaagtatc ttccatgacc cgtacgggat
     9421 tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca acgggccaat
     9481 aaaataaatg ggcctaaatg gactcgaacc atcgacctca cgcttatcag gcgtgcgctc
     9541 taaccagctg agctataggc ccataaaaac taaaagcggg tgacgagaat cgaactcgcg
     9601 acaacagctt ggaaggctgt ggttttacca ctaaactaca cccgcataat ggcgcgggac
     9661 agaatcgaac tgccgacaca tggagcttca atccattgct ctaccaactg agctaccgag
     9721 ccaaacggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa
     9781 cccctacacc acggaaccaa tgtttttagt ttttaattgc gggagcagga tttgaaccta
     9841 cgaccttcgg gttatgagcc cgacgagcta ccagactgct ccatcccgcg ataatatgaa
     9901 aaaggaggat aagggattcg aacccttgca cggttttacc cgcctgacgg ttttcaagac
     9961 cgttcccttc agccggactt gggtaatcct ccatgtaaaa ctatattaaa ttaatgaccc
    10021 gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa
    10081 cgggccaggt aaaacaaaat attgaaacgg agaaggaggg atttgaaccc tcgcgccggt
    10141 ttcccgacct acacccttag caggggcgcc tcttcagcca cttgagtact tccccaaaac
    10201 caataagatt ttgtactaat gggcctaaat ggactcgaac catcgacctc acgcttatca
    10261 ggcgtgcgct ctaaccagct gagctatagg cccataaaaa actaaaagcg ggtgacgaga
    10321 atcgaactcg cgacaacagc ttggaaggct gtggttttac cactaaacta cacccgcatg
    10381 gcggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    10441 tacaccacgg aaccagtgtt tttagttttt aattgcggga gcaggatttg aacctacgac
    10501 cttcgggtta tgagcccgac gagctaccag actgctccat cccgcgataa tatgaaaaag
    10561 gaggataagg gattcgaacc cttgcacggt tttacccgcc tgacggtttt caagaccgtt
    10621 cccttcagcc ggacttgggt aatcctccat atgaaactta caacgccaca atggaccttg
    10681 taggactcga acctacgacc ggacggttat gagccgtctg ctctaaccaa ctgagctaaa
    10741 ggtccaggct cttaaaaatc gcggcggagg ggatcgaacc cccgacctcc cgggtatgaa
    10801 ccggacgctc tagccagctg agctacaccg cgaaaaagat attcattatt taaatgaaat
    10861 ggagcctagc gggatcgaac cgctgacctc ctgcgtgcaa agcaggcgct ctcccagctg
    10921 agctaaggcc ccgtgtttaa atttataatt atcgggaaga caggattcga acctgcgacc
    10981 ccttggtccc aaaccaagtg ctctaccaag ctgagctact tcccgtttaa ttaacgcgcc
    11041 caagaggagt cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct
    11101 atgggcgcat atttgaaatg ccgaggaccg gaatcgaacc ggtacggtga tcactcaccg
    11161 caggatttta agtcctgtgc gtctgccagt tccgccaccc cggcgcgatt gctttggcaa
    11221 agcggaaaac ggggttcgaa cccgcgaccc ccaccttggc aaggtggtgc tctaccactg
    11281 agctatttcc gcgtgtttaa agatgccggc taaaggactt gaaccctcga ccctctgatt
    11341 acaaatcaga tgctctacca actgagctaa gccggcatgt aaaacttaaa ttatgcgggt
    11401 gaagggactt gaacccccac gccgtaaggc gctagatcct aaatctagtg cgtctgccaa
    11461 ttccgccaca cccgcaaaaa tatgagccgt acagggctcg aacctgtgac cctctgatta
    11521 aaagtcagat gctctaccaa ctgagctaac ggctcatatg gaggttaacg ggatcgaacc
    11581 gctgaccctc tgcttgtaag gcagatgctc tcccagctga gctaaacctc catggataac
    11641 tt
//
LOCUS       DAYYCQ010000087        11567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000087 DAYYCQ010000000
VERSION     DAYYCQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.088"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            374..580
                     /locus_tag="ACHAYF_002394"
     CDS             374..580
                     /locus_tag="ACHAYF_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8579784.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(782..1783)
                     /locus_tag="ACHAYF_002395"
     CDS             complement(782..1783)
                     /locus_tag="ACHAYF_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8579785.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1788..3701)
                     /locus_tag="ACHAYF_002396"
     CDS             complement(1788..3701)
                     /locus_tag="ACHAYF_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8579786.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3869..4375)
                     /locus_tag="ACHAYF_002397"
     CDS             complement(3869..4375)
                     /locus_tag="ACHAYF_002397"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8579787.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4535..4975
                     /locus_tag="ACHAYF_002398"
     CDS             4535..4975
                     /locus_tag="ACHAYF_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8579788.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5001..6116)
                     /locus_tag="ACHAYF_002399"
     CDS             complement(5001..6116)
                     /locus_tag="ACHAYF_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579789.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6161..6517)
                     /locus_tag="ACHAYF_002400"
     CDS             complement(6161..6517)
                     /locus_tag="ACHAYF_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579790.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6815..7789)
                     /gene="sgrA"
                     /locus_tag="ACHAYF_002401"
     CDS             complement(6815..7789)
                     /gene="sgrA"
                     /locus_tag="ACHAYF_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8579791.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7985..8824)
                     /locus_tag="ACHAYF_002402"
     CDS             complement(7985..8824)
                     /locus_tag="ACHAYF_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8579792.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9009..9896
                     /locus_tag="ACHAYF_002403"
     CDS             9009..9896
                     /locus_tag="ACHAYF_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8579793.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10490..11184)
                     /locus_tag="ACHAYF_002404"
                     /pseudo
     CDS             complement(10490..11184)
                     /locus_tag="ACHAYF_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11168..11566)
                     /locus_tag="ACHAYF_002405"
     CDS             complement(11168..11566)
                     /locus_tag="ACHAYF_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8579794.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3446 a         2302 c         1735 g         4084 t
ORIGIN      
        1 ttggtgaatt aagtatttcc ttgataacac tggcttttgg cctcatttta ggttatcatt
       61 tgtaatttta tttcacagtt gatatgattg ttatgtaaaa taaaaaagtt taggatacat
      121 cgaatttttg gggaaattct tttgggggta tcctaaatct tttctattat attcccatat
      181 tttctattat tggtctagac gtatgatggt aaactttgta cctatctgat tgctgaatca
      241 ttaattgctt atcattagcc atctttgttt caagttcgct agatgatcat tttattcaca
      301 tcctaacaaa cttgtcacat ttatagattc ttaatctatt tatcatacaa ttataaaaaa
      361 ggaggtgctg cttatgaaag ctatccatat ttctttgctt tttttcgcta gtgcattgat
      421 tttatttgct attcatcttt gttcccagcc ttatatcaat aacaacggaa tattagtgga
      481 accagcattc ttttgcttac cactcggctt tttgtccctt cttgcctccg caggcttcgg
      541 atttgttgct ttttttaaac gatcaaaaca acaaatctga cttataaaat ttacgtaaaa
      601 gagatcgtga caataatcgt gtgatattcc caaatttcga ataaaaggtg ccccttgcca
      661 tctctaactc ctcagtcaca agttacaata ttcataaacc atgaaaagct tgtttattga
      721 aattgctcct tgtgacttgg agctaagtga cagattacaa cctcttttgc atcataagac
      781 tttactcctt gaaaaacatt actaactttt gatatgtttc ttgcgcttgt ttattgctga
      841 aatcaaactg atattcatgg ttcaaaacag catttttctc cgcataatct acttttgtga
      901 ctttcacccc cgctttctca aatacagaag ttacttcttc catttgtttc gtaaacgtat
      961 ttgtatttcc atccgttaag aatgtgtttt gcgtccaacg atccgcatgt ctgaccaagg
     1021 ctaaactttt gaccttctct gattgttcaa agtccttatt atcaaagtaa gcccatccac
     1081 atttttcata aagaaagcta gagattttat cacttacttg agtaagtgca gaaggatcca
     1141 acagaccaga caaagaaata aacttcgtaa tctgcttgtt ctccaaagtc ggatcaagat
     1201 gatcagcttt tttaatttct tcattttgaa gtgctaacaa atattctcca ctaatttgtg
     1261 cacctgccga atttccacca atcgaaatat tcgaccaatc tgcttgatct tttatctctt
     1321 ttttagctgc ttgaatggct tcgttcaatt ggataactgg tgtaggatgt tttgcttccg
     1381 gtgatcgttc ataatctata tttacgatga tttgctgcgt gtctgaacat agatattcca
     1441 aataatcacg acaatcattt ttatcccctc ctacatatgc gcctccatgc atccagaaaa
     1501 gaatcggtaa ttttttcttt tccactttcg gtgtgtagat atccatatta ttatttttgt
     1561 actttgaagg ataggatatt tcttttttta tatcgatttg ttcgattttt tgctggaaat
     1621 catctggtcg ggcatatact tgttcttttt cacttgtgtc aaattgtttt ctaacaagca
     1681 agctaatcgg ctttggtgtg atttgattaa tcaaaaatac agtgactaaa ataactacga
     1741 tgataactgg tactgcaatc actattcgct tgaaccattt cattcattta ccctctcttt
     1801 cttcgccaaa tccatgtgat aatcgtgcta acagataaaa atccacctga ataaatgaga
     1861 agcgaatgtt tcttttctcc agttttcggc aacattcttt tttttgcatc tgtctttttt
     1921 tgtactttca ctgtcatctg aatcggttgc gtatcatcac ttgattgatc ctctcgaggt
     1981 tcattctttc ccttattgtc tgcattctct gtcggatctt gcttttttgg tggagctact
     2041 gggatttttt tgtaatagaa aataatagtt ggtatcgtct cattaaaaat cccttcagtc
     2101 ggtccttcaa catttgctaa ttcgtatcct tcgaatgtct tttgctctgt catataattt
     2161 tctccataag ctccgtaaag ctggatttcg tcctggtctt tgagtagact tttattgttt
     2221 tcatccacat actttacgtt tatctgatca tggaacacta ctttcaaaaa gtccgttaca
     2281 tgtttcgtgt aggcagcatg gtcaacttcc tgtgcctttc catgccctgc tccttctact
     2341 aataaaagat ctttataccc tcctgataac tcatataatt tttgcgcgtt agacacaggt
     2401 actacatcat ctgctgttcc atggataatc aatttaggca atgaatctga cattccaatc
     2461 gtaggtagag cagcttttgt atccaaatgc atctcctgat caaaatagta ttgtttcaaa
     2521 aggcccataa ataaatcttc atgctcttga tccccaatga tgtctaactg attttttatg
     2581 gcatccggta gtgcgggggc tactgtctgg ctgtacgtaa aacgtagttg ttcatcaatt
     2641 gatgcaaatc cgcaattttc aataattcct ttgacattga caggaatatc tttcgctaat
     2701 acagacatca tcgtagctgc tcccatcgaa ccaccataaa gtaaaatctt ttgagatggc
     2761 cgctctcgaa cttcttgatt tatccagtaa aggacgtcat ctgactcata ttgtccatag
     2821 gtcacataat cgcctccgct attaccagtc gctctggaat caacaaataa taaattatac
     2881 ccggccttat aaaaatcatt gtattcttct gtaacgatcc cgttattcca attccctcga
     2941 aatccgccat ggatcatgac tgttttatct gaaccttgat cgacataata tgcatataca
     3001 tccatcattt tgccatcttg tttgtttagt acagtttgat gaagttgttt tgtatttgga
     3061 tagaagcctg tgttttctgt aggcgttgtc ccatcatctg tacctccaag catcgacatc
     3121 aacacactgt tttttgcaaa agcgatatcg tacaatagat aagtagcaat cagatgtttg
     3181 ataatctctt tatctacacc ataatcatta acagcaagct cagtgactaa taactctatt
     3241 aattttgact tgatgtcttc tgttggtcca ttaagtattt ctgtcatctg tttaccgaag
     3301 taattcaaat attctttacc attgaaattt tcacctttac cgatatcatc attttctgtc
     3361 ccattctctt tgtcacttac tttagcagaa gaggattcgt cgtttgacat ttttgtagct
     3421 gttgcttctt ctgtttgttg gttatctgtg gtagcggtcg tttcttgatc tgtgctagta
     3481 acagatgtct caaccgttgt ttcagtattt gtttctgttt gtgaagtact cgatttcgtt
     3541 tcatttccag catcagtagt ttctgtggtt tctacctccc gagattccga ttcggaagaa
     3601 atgctctctc catatactgt ccattgcggg acgcttgtac ttacagcaaa taagatgacc
     3661 aacgctccct ttaatacttg ttttgttctc aagctattca ctccttttta tgtttctagg
     3721 agaattatat gtaaacgcaa acatttttta tacaaaaaat aaatggttta tcccataaaa
     3781 atagaacagg attattttct cattttacgt aataaagcag aatggataaa ccagtatcca
     3841 ttctgctttt tttaaacgaa gaaacaactt attgctccaa aaatgtcaga aatcgtttat
     3901 cccaaatttt ctcgtaaaaa gcgattgttt cctttgcttt cataaaagga ttatcataag
     3961 ttgtcgttgc tcctctacac atcataagct ggtatcctaa tccatgagca aacttcacag
     4021 tcgtatctat acaatactct gcttgtgccc cacataattc gatcgaatga cagtccaatt
     4081 cagttaataa aacactcaaa ttagtcttat aaaatgaatt cgcatgggtt tttctcacaa
     4141 aataactgtg ctcaggaata gataattccg acaatagctc ccatggtttt gtttgtggca
     4201 ctaattgagc atcttcgtgt tgaacgaaaa taactggttt cccaatcgtt tcgtatgttt
     4261 tgattctttt attcactaat gctgtcactg tttctaattc gtgtatcggc gaagaatcaa
     4321 aacatacccc tttttgtaaa tcgatcacta ctattgcgtc ggctgtgttt gacatacttg
     4381 tgccttcttt cttttttctt gatttaatag tatttcttat ctagctagtt ccattatacg
     4441 tgacaacatt ttcttatttt acacctctaa atggcacttt tcgactattt cttcatgtta
     4501 ttatttcata aatacttagc tattggagga atgtatgatg attaaattgt atacaggttc
     4561 tggctgctcg tcttcaagac gcgcaaaacg ttggcttatc gtccatgata taccgtttat
     4621 cgaacagaac ttaattcgtc aaccactaac caaaaatgaa tttatccact tactaaaatt
     4681 gacaaacaac ggcgtgaccg aaatgctctc tactcattct attagttata aaaatcttgt
     4741 tcacagaata gatgaactca gtttgagtga actatatatt gagattcgta catccccttc
     4801 tttattgaaa aaaccgatta tctacgacca atctgcaaaa cttttgactg gatttagcga
     4861 agataaaatc aggatgttcc tttctaaaac taaaagaaag caagagctca acgaactctt
     4921 gctttctggt catcttgtcg attttaaaga aaataatctt tttgcaatgt cttaattttg
     4981 gcataaacaa tctgcttttc ttaattaacg aaatccaatt tttccaatat ggatctattt
     5041 ttctcattcc tgatttttat gatttcagct ttcagacgcg cccaatcatt ttttgtaggt
     5101 tgtccattcc agtaaaatat ctgtgtattc gtttgatcat atgccttttt gtcgatagga
     5161 taatcgctaa tgacaatatc aggtaactga tcagcggatg cactatattt tattgggcgt
     5221 tcagtcatag ataatatcct tttctttgcc cataattcct gtgttttacc atactttgat
     5281 tgaatataga atatgacagg ttgttcgtgt tttataaaaa tatccctgac taacatacag
     5341 tattgaaaaa tcaaccgtct gttcttttca taataagtat taagctctgg aattctgcga
     5401 gctaccccac taaaaaactg ttgcatgatt ggatacatca ctggaaatat atgctgaaaa
     5461 tctctgtctt ctgctgattt tccaaaagca tccaccttct tttttgtccc aaaaaccctt
     5521 gaatacaaat ggatcattgc caaattgaca tatagataat ttttttctga agttgtaaaa
     5581 caaatcttga agtttttttc aaaattttct acaatagacg attcaatcca gccccaactt
     5641 tcttgtactt tatctggagt atccaacaaa tcttcttcca aataggtgtt cattacacca
     5701 aacataaaat aaaggtaagc ttgctctgtt ggagtgatgt gattcatcga ccagaagttt
     5761 gcttcaaaaa aaggctctag tatgtaatgc gtaaactctt cttttgttat caatggatgc
     5821 acaaatttgt tcatctcttc tgggacatac gtaatcgagt ggccttgttt cattcgttgc
     5881 aacgaaattc ttagtaggct gatccatttc tttctatcta tactacgcgt atatggcgca
     5941 tgtttcgcaa atttttctaa tagttgacgt ttatttttta tacttgataa tatatcagaa
     6001 tgattttcgt cgaatgactg ccaatacatc aagtgaaaaa agtatcggat acggtattct
     6061 tctccttcta tcggattacc ttttttcaag ctgcaagata aatgatagtt tttcaaaata
     6121 gtattaagtt ctcttaattt tctttgaatc gtacgcaaac ttatataatt ttttgactaa
     6181 gattttctaa ggattgataa gtacctttgt aaagtgaatc cagtaaaata taaccaatcg
     6241 ttccctctaa aagtatagaa taaactttac tcagtaaaaa ctgagaagaa aactcgactt
     6301 ttatcatctt gttttctgaa agaaaagata atttatattc tggatacctt tcgcaaaacg
     6361 attctgcttg ttgaatcacc gaattcatct tagccagggt gtagtttaga tctaaagcga
     6421 tatccttcac cacatataat ttctcgtctc tttcataaca atagttcaaa aattgatata
     6481 cttgcctttc attgatatca ataaattctt caaacaaagt acatactcct ttttatctat
     6541 tatttagact tattccgaca gctacatgaa taacgtacaa atagattcgt tactgatttt
     6601 tcagagatac taccatgcgc agtgtgcatt gtcaattatt cttgctctta ttttccattt
     6661 gtgttcctgt tatttcctgt ttctcaaata tgttagtaaa caaaaataga tctggcaaag
     6721 ctacaacttc gccaggcaat acagcactct cgaatgaaat attcactcaa aaatctatcg
     6781 ttttattcaa ttttagatct gttgctttta ttctttattt cactcgatgg aagagagaac
     6841 gaaaatagct tgctcctaca atcaaaatga gtaacacacc catccatgta attagcttgt
     6901 tcaaggttct actaccagtt tctggtaaat cgctttttct attatctgtt tctttcgctt
     6961 tttcttgatg atcaattcca cgagaaacaa aatcaggctg ttttagcaca ctttttaggt
     7021 tgctccctgc atttctcttt tgttccttag ttggtatgat aggtaccaaa tcaagttcat
     7081 tatttacttt tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactc tcactactca
     7141 tgcttcctgt gctactttca ctactcgtac tctccgtact gctttcgctg ctcgtgcttc
     7201 ctgtgctact ttcactactc gtactctccg tactgctttc gctgctcgtg ttttctgtgc
     7261 taatttcact gctcgtactc tctgtactgc tttcaccgct cgtgttttct gtactgcttt
     7321 cactacttgt gctatccgta ctgcttggcg gtccaacaac tgtccaactg cttggtttca
     7381 aattctccgt gatcgtcatc tgtccatcat aaaaaacaat caaatcaact ttatctttac
     7441 tcttgatctc atcaatcgat agttctgcag aaatcattat tgtatactca ataccagcgg
     7501 tccattgtac gtcttttaaa taaacttccc caataacttt ttctgaactt ccattaccat
     7561 cccaaccata tgttagaaca gcatgtacat cactagcatt cacgcttttg tttggtttga
     7621 ctggaatagt aatgacttgt tgtgtcatgt cataaacaat ccgatcagtt ttcacagtaa
     7681 atgaaagatt ctcatttgcc cgttcatttg cagaagtact gattccaatc attgctgcaa
     7741 ttatactcag cccaagaata acaaataata cggtcgctgt tttcttcatt tctatcactc
     7801 tccatataat taccttctat ttgataaagg aattatataa aatgaaaatg atgagaacac
     7861 gacagtcagt tgtgaaaatc taacaaaaga tgtcgtgtat tcgcttgaat ttgtttactt
     7921 tttcgtttaa tcaatgacag acttaaaaaa gcaagaccga tatcagcctt gctttcttca
     7981 tcttttattg ataaacagca gtgtatttct tcatccgctt tgttttatat gcatagagta
     8041 acaaaccaac aatcaagact aacaagaata atgtagttga tatagatgct tcagccccaa
     8101 agtttccccc agtaattatt gtattttgcg attggatcag agtggagaaa aaaccttgtt
     8161 cttgcgttcc gctgactggt atacccaaaa tagctccttg tgtccaattc caagtaatat
     8221 gagcaccaat agccgtaaat aaacttcctg tactgtagat aagtgcgctg aaaaagcaac
     8281 ctgctaaaaa gatattgaaa actgccagaa atgtcatgtt aggattcaat ccgtgcaaaa
     8341 gagcaaataa tgcagcagat ccaatcattg ctattaacgg accaaatcta agcatctgat
     8401 gctgaaaaaa gccacggaat aaaagctctt ctgatgcccc cactgcaata aataaaataa
     8461 aactcgttat cagcggcaca atgtttgcgt ttcctgaatc taaccgatac gcccccgtca
     8521 aatataatgg actagtaata agcagaaaca ctccaattcc aacaacgaga cctcccgcta
     8581 aataccccca gtcttttcct cgaacagaaa caccataatc ttctattctt tttccttcga
     8641 ccttactagc aagaaataaa gctagtaaaa aaacagctaa atgtccgacc gtaccggcaa
     8701 ttgctccaat ccattcacta tttccaataa acatatccgc aagtgcatta ttgacaagaa
     8761 ttcctaaaac aaaaccaaca aagactatta aaacagatcc aatcaaccga ataatcctga
     8821 tcatctttca tccctccttt atattaagtt taacaaccct tttctttctt gtctcgtgaa
     8881 aactatgtaa atgttctcca tcatagagag aatagatttt tacgaactaa gaatatctac
     8941 agcaacctta cgaaaatgaa agactgtctc cctaattctt ctgttagact gtttttaata
     9001 aggaaaggat gggaaatttt ttgaaaaaac aactactaat tatgtgcctt tcagcatgtg
     9061 caattttact attctcaggt tgtagcaagt ccaatgaaac gattgcaacg tttaaaggtg
     9121 gaacacttac agttcaggat ttttatgata acccaaaagt taaatcaaat aatcaaacac
     9181 tattaaaaaa aatgattgtc ttcaaagcat ttgatgatgt atacggaaaa gagatttctt
     9241 caaaggaagt aacaaaagaa tataatgaac aaatcaaaaa actcggtcca aactacaagg
     9301 aacaattaaa agcagttggc caaacagaag agacatataa gaaactcttt aaacagatgc
     9361 tggctttcca atacggtttg aaagccaatg taaaattaac agataaagat ctagacactg
     9421 cttggaaaga attctatcca gaagttagta cacagatcat tctattttcg actaaagaag
     9481 aagcagataa agcaaaaaaa gaagcaaatg aaggagaaaa tttctccaag cttgttcaag
     9541 cttatggaaa aaataaaaca ttaaaggaaa ctgatggaaa aatgaatttt gattcaacca
     9601 atccagagat tcctactgaa gtaaaaaaag cagcatttaa actaaaaaac ggggaagttt
     9661 ctgatatcat tcctgtaaca gatccaacga cttatcagca aagctattat ctagtaaaaa
     9721 tggtaaaaaa acaggataaa ggttctaata aagacaaata caaatctgag ctagaaaaaa
     9781 ttgctaccga agcaaaaact cttgatacgg aattcatgga taaaacaatc cgtaaagtga
     9841 tgaaaaaaga caatgtaact attaaagatc cttatgtaaa aaatatattc aaataaacaa
     9901 atttgaccca attggggatg tggcaaaatt tgagaagtga tttttggaaa ttccttctta
     9961 tttttgaggc aaaccacttt tgccacaact tcatcggggc ctttttttat ttgtaccaaa
    10021 acaaatcact ggatttgtta caatcatgag gatataattt aaaaaagttt tttatttcta
    10081 tcacatgaat caatgcgcca ttaagtagat tctttcatgc ttccctattt tttcacttgc
    10141 cgtatcgctt caaaaacaac tatcataaac tcaaactcga ctgaaaagtt attttaaaaa
    10201 agactaaaaa tatcccttct ggacttgttc ttcttattct gttatactag agaaacacga
    10261 aagatatttt tatgcgacac ttttcatttg tttttatgaa atttattcaa aactggcgta
    10321 acttcagagt tacaaggatg tattagaggt tagggaatac atcgtttaaa tgagaaataa
    10381 gtaatagtta gtaatagtca cccaaacttt aaattaatga atattgaatc gtcagatagc
    10441 ctcctcgagt tttcgagaag gctatcgttt attttatgga gaaattgatt cagaagtaat
    10501 cagcgacctt ttttagttca ccaatcgttt ctgcttggaa agtttgcacc caatcttctt
    10561 ggaagcccct cactgcttta tcaatcgagg gcatctctaa tacttgatct agtagctctt
    10621 tacctaacgt tatcgctgag ctccagatcg aatggctgta gtgacttgtt cacaattttt
    10681 gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag tccgcaacga
    10741 atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat aaggtgcaat
    10801 ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa tagctgtacc
    10861 ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg cttgattcac
    10921 cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag catcctctat
    10981 catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc cgattatttt
    11041 tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca gaattgtagg
    11101 atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac tttttgtatt
    11161 tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct tgatcgattt
    11221 taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac attgttcctg
    11281 gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat tcattcaaat
    11341 tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca aaacagagct
    11401 tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa caataatctg
    11461 ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc ctgaatactt
    11521 ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatc
//
LOCUS       DAYYCQ010000088        11214 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000088 DAYYCQ010000000
VERSION     DAYYCQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.089"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..1435
                     /gene="lysA"
                     /locus_tag="ACHAYF_002406"
     CDS             176..1435
                     /gene="lysA"
                     /locus_tag="ACHAYF_002406"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8579795.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1422..2222
                     /gene="dapF"
                     /locus_tag="ACHAYF_002407"
     CDS             1422..2222
                     /gene="dapF"
                     /locus_tag="ACHAYF_002407"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8579796.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2225..3106
                     /gene="dapA"
                     /locus_tag="ACHAYF_002408"
     CDS             2225..3106
                     /gene="dapA"
                     /locus_tag="ACHAYF_002408"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8579797.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3103..3819
                     /gene="dapB"
                     /locus_tag="ACHAYF_002409"
     CDS             3103..3819
                     /gene="dapB"
                     /locus_tag="ACHAYF_002409"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8579798.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3825..4520
                     /gene="dapD"
                     /locus_tag="ACHAYF_002410"
     CDS             3825..4520
                     /gene="dapD"
                     /locus_tag="ACHAYF_002410"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8579799.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4536..5537
                     /locus_tag="ACHAYF_002411"
     CDS             4536..5537
                     /locus_tag="ACHAYF_002411"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8579800.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5534..6895
                     /locus_tag="ACHAYF_002412"
     CDS             5534..6895
                     /locus_tag="ACHAYF_002412"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8579801.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6855..8006
                     /locus_tag="ACHAYF_002413"
     CDS             6855..8006
                     /locus_tag="ACHAYF_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8579802.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7981..9150
                     /locus_tag="ACHAYF_002414"
     CDS             7981..9150
                     /locus_tag="ACHAYF_002414"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8579803.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9325..10704
                     /locus_tag="ACHAYF_002415"
     CDS             9325..10704
                     /locus_tag="ACHAYF_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8579804.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10985..11214
                     /locus_tag="ACHAYF_002416"
                     /pseudo
     CDS             10985..11214
                     /locus_tag="ACHAYF_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3734 a         1953 c         2502 g         3025 t
ORIGIN      
        1 aatagttcgg gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc
       61 gaaatatact agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct
      121 ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac
      181 agaattgata attggcggag tcacagcaag cacattggca gaaaagtata agacacctct
      241 gtatgtgtat gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt
      301 atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat
      361 gctgcattta gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta
      421 taccgctttg caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac
      481 gccagaggaa ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt
      541 gatggaagtt gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat
      601 gcttcggttg aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat
      661 tgattcaaaa ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat
      721 aaaagaaagc agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt
      781 tgatgttact gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga
      841 ccaacctctt tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca
      901 gccgatccca gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga
      961 aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga
     1021 agctggtaca acactataca cgattggttt catcaaaaag acaccacata aaaaatatta
     1081 ctttgtagat ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga
     1141 ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa
     1201 aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg
     1261 tgatctttta gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa
     1321 tcgagcgaca cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg
     1381 cagacaagca ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata
     1441 atggctgcgg aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat
     1501 tagccgcttc tttatgcaca aaagaaaaat atgatataga cggattgatc gccgtaaaaa
     1561 tcgatccgtt ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa
     1621 atggcattcg ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat
     1681 tcaacgtaga gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt
     1741 attgcagcgt agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa
     1801 cttatccaat aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt
     1861 tatttttagg aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga
     1921 gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact
     1981 ttgtagaagt agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt
     2041 ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga
     2101 aaatttcagc agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg
     2161 aggatgcgat acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat
     2221 gaagatgata aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt
     2281 agattatgct ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt
     2341 attagtttta ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat
     2401 ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac
     2461 gaataatacg aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc
     2521 acttttggtc atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt
     2581 tacagcaatt gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac
     2641 aggcatgtcg attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg
     2701 tttgaaagaa gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga
     2761 agaattcttc ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg
     2821 aagcggtgta ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa
     2881 agatacgcag gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact
     2941 gatccatgcc ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt
     3001 agacttacct tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa
     3061 acaattagct cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt
     3121 ggcagtggac agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc
     3181 cttcttttac ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa
     3241 ctgcttattg atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca
     3301 tatcaacttc cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata
     3361 aaagaacatg ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg
     3421 gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg
     3481 attgaaaaac atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt
     3541 caaacaatcc aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga
     3601 gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat
     3661 gtattgtttg ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga
     3721 atctttgctg caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat
     3781 tacaaattag aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa
     3841 tcatccgcta cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa
     3901 ccgagccaat cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt
     3961 ttggtgacta tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa
     4021 tcgaaatcga aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta
     4081 atgctcgtat cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg
     4141 tgatcatgat gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg
     4201 atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag
     4261 gagctgtctt agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg
     4321 gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag
     4381 tcgtggcagc aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa
     4441 tacctgcacg tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg
     4501 ctgcgttacg cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg
     4561 tgggggcaac cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc
     4621 cagtaggacg attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca
     4681 agaaaaagaa acacactgta caggaattga cagaaacttc atttgaagga tatgatttag
     4741 cgctattttc tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg
     4801 gactatttgt catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg
     4861 ttccagaggt caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt
     4921 caaccattca agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag
     4981 tcaattattc gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc
     5041 atcgtacgca aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga
     5101 ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa
     5161 atgagacccg taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg
     5221 tgccaatcga aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga
     5281 cggaagaaat tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag
     5341 cagcacataa atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga
     5401 ggatcagaaa agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca
     5461 tacgcaaagg agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag
     5521 cggaggtggc aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc
     5581 tcatgaagag caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt
     5641 gatcgttgtt tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact
     5701 ttatttgatt tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat
     5761 ttgtgatcgt ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga
     5821 actagcacgc atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag
     5881 tagaggtgaa tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga
     5941 cgcaaaagac ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca
     6001 ggaaatcaca aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc
     6061 gaaccctgca ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat
     6121 actggcgaag atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat
     6181 gatggctgat ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga
     6241 actgagagag atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt
     6301 tcaagaggct gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac
     6361 aaaaatatcg aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa
     6421 ggattttctt tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg
     6481 gaaagcaatg agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat
     6541 cgacaatatc ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac
     6601 gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt
     6661 gatctctgtc gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc
     6721 gatattgaac aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa
     6781 tctgatcatc ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg
     6841 gatggtgaat acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg
     6901 ggtttcatga attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcgaggccg
     6961 ttatccatga aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa
     7021 cgattgcttt tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt
     7081 ttgcttctaa gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc
     7141 ttttagggtt gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac
     7201 tgatctatca gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat
     7261 tgttggataa atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta
     7321 agggagaagt tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta
     7381 tcattaaagg ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg
     7441 cagctgtacg atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga
     7501 atcatctaat gaaatttggc caaatccaag gtggaacgat ccgaaatgtt ttagctggag
     7561 aagttattgt atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga
     7621 ggggattaga ccatttagca gaacagtttc actctacagc tactatttct tttcgctata
     7681 atgacggcta tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa
     7741 acaaactaca tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc
     7801 aacaatatcc atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc
     7861 cgacgttcga ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc
     7921 tttttacaga aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata
     7981 atgatgaatt tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata
     8041 cgacaattcg atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa
     8101 ccggattttc caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac
     8161 ttttctcact ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt
     8221 cagcaacagc gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt
     8281 gcaacggaag cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt
     8341 attccagcgc ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg
     8401 atcccgatag atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca
     8461 tttgaacaat atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact
     8521 gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg
     8581 gtatttgtta taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc
     8641 atagctactt atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg
     8701 atgactggtt ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt
     8761 aaagtccatc aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa
     8821 gcgctaataa atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga
     8881 gactatctgg tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca
     8941 ttttacttgt tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta
     9001 gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga
     9061 gaaggttata tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa
     9121 cggatcaaag actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt
     9181 aattttgcgg tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc
     9241 gtttcttttg attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa
     9301 agaatattca aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct
     9361 catggagggc atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc
     9421 acgctatttg aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta
     9481 gaagacaagg taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa
     9541 tccaatgttc atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca
     9601 gtaacggtta ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt
     9661 ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc
     9721 tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct
     9781 attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc
     9841 cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat
     9901 ttagatgctg agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt
     9961 ttgacaaatg caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca
    10021 agtgaaaaag aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca
    10081 gaatggttaa aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac
    10141 ttgcaaacga atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc
    10201 ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat
    10261 gtagtaaaac atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc
    10321 tctgcattaa gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca
    10381 gcaaaagcag gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat
    10441 gtaccagaag aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc
    10501 catcaattgc taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta
    10561 ttggcactag cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc
    10621 ttccagccag gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta
    10681 ggagaagaac catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc
    10741 tgccttgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg
    10801 gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact
    10861 gtgacacgat ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt
    10921 cggtattttt tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt
    10981 tggatggcaa aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct
    11041 taaaattcgg gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa
    11101 ccgcggtttc tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa
    11161 agtaacggca tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DAYYCQ010000089        10691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000089 DAYYCQ010000000
VERSION     DAYYCQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.090"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..2000)
                     /locus_tag="ACHAYF_002417"
                     /pseudo
     CDS             complement(365..2000)
                     /locus_tag="ACHAYF_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2439..3308)
                     /gene="scrK"
                     /locus_tag="ACHAYF_002418"
     CDS             complement(2439..3308)
                     /gene="scrK"
                     /locus_tag="ACHAYF_002418"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8579805.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(3328..4449)
                     /locus_tag="ACHAYF_002419"
     CDS             complement(3328..4449)
                     /locus_tag="ACHAYF_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8579806.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4451..4846)
                     /locus_tag="ACHAYF_002420"
     CDS             complement(4451..4846)
                     /locus_tag="ACHAYF_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8579807.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4865..5662)
                     /locus_tag="ACHAYF_002421"
     CDS             complement(4865..5662)
                     /locus_tag="ACHAYF_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8579808.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5664..6452)
                     /locus_tag="ACHAYF_002422"
     CDS             complement(5664..6452)
                     /locus_tag="ACHAYF_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8579809.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6466..6942)
                     /locus_tag="ACHAYF_002423"
     CDS             complement(6466..6942)
                     /locus_tag="ACHAYF_002423"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8579810.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(7014..9671)
                     /locus_tag="ACHAYF_002424"
     CDS             complement(7014..9671)
                     /locus_tag="ACHAYF_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8579811.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(9735..10094)
                     /locus_tag="ACHAYF_002425"
                     /pseudo
     CDS             complement(9735..10094)
                     /locus_tag="ACHAYF_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(10272..10592)
                     /locus_tag="ACHAYF_002426"
                     /pseudo
     CDS             complement(10272..10592)
                     /locus_tag="ACHAYF_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         3421 a         2128 c         1533 g         3609 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt
     1981 atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag
     2041 tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa
     2101 aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct
     2161 taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga
     2221 tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata
     2281 tgactattta ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc
     2341 tgggtatcat gagtgaatga tattgctgaa tacaaatagt ccgaccacat taagttgatc
     2401 ggacttcata agcattttat tgactaatac ttttttgctt aagaattgat tctaacatct
     2461 tttgctaaag ttaagcatcc tatagttcct gcattatctg ctaacttagg tgtgacaatg
     2521 tattcttcga tatttggcgt atcgacatag ccgttaatta gttctaaaaa acgtctttgt
     2581 actttctttt tcagatgttc ttgttgcatg acaccaccac caaaaattat aatatctggt
     2641 gaaagcatca gggtcgtatt acaggcacat tgtgcaatat aatccgcctc gatttcccag
     2701 aacggatctt ctatagaaag atcttgtccc ttggttcccg tacgcatctc gatagcgggt
     2761 ccagatgcca acccttcgag acaatcatga tgaaaaggac aagtaccaga gaaagtatct
     2821 cctgaatgtc tcttaactaa cgtatgcccc atttctggat ggctaaaccc ttcaacaaaa
     2881 atcccatctt gtatagctcc tccaccaatt ccagttccaa cagtgtaata aacaacactg
     2941 gataaacctt ttccatgacc tgaaacatac tccccgtaac aagcagcatt cacatcggtt
     3001 gtccaggcaa ttggaatttc gaaatgctta ttcaacgttc caacaaaatc aaaatttttc
     3061 caggcagttt ttggagtaga ggtgatatgt ccataggtct ttgattcacg atggatatca
     3121 atcggtccaa atgaccctac tccaatgctt tctaattgtt ctttatagtg attaaagaaa
     3181 tcaattacca aactcattgt ttcattcggt gtagttgtag gaaaactcac tctttctaaa
     3241 acctttaaat catcattgcc aatggcacaa acaaatttag tcccacctgc ttctattgag
     3301 ccatacattt cttactccac cgtcctttta tctaggaatt cgttcttccc tgtctaatag
     3361 tttttcgaaa aactcatgtg gttccttttg gtaccaatag gcaacagaag aaatatcgtc
     3421 ttgacgctca taaagcttga tgtcatcatt tcctagggtt tggaaagtaa cacgtaaatt
     3481 ttctttgaaa ctaatggggt cgggtaaatg ccagcggtac aacccatgca tcggtaaagc
     3541 atcatcgcca aaaccatgaa ctggatttgg attaggtttt ccagcactaa aataatcccg
     3601 ggtgtgatct cttttagatt gaaaaggata gcccataaat agcgtattaa aattttgagc
     3661 atatgccctt tctccaaagt tctttttatg gaaagcccaa gcgccgccaa aataatcttc
     3721 tgctcctgta gatgttattg tagggaaatc ttcatctcca tctatataaa acttaaattc
     3781 tccttccccc caccagtatc tttccaatgc gcacaaagca aagtaggttc ctacataata
     3841 cccagtgcct ttgattccat caataagggt gtaatctttt ccttttgtcg tgatgggttc
     3901 acggttccat ttagcatgaa aatacatcgc atcatccggt acttcagtca atgcataatt
     3961 aaatgtataa aaaatgtgct taataaactc aggatgttca ttcgttaaag taatcttagc
     4021 tttttttcga aatggcatct caaaatagca attcatccct gatgttggat tcactacgat
     4081 tggtaaggaa ttcacttcgc atcttgtccc aaacccatta caaaaaaaat ctcctaatgg
     4141 actttctaca gaaggagtgg attcatcatc ccaataaata cgcaaaatta aatcacgtaa
     4201 gacgaaactc ccagcgattg ttttgtctgt cactgtcatc caaaaatgac gtaaaattcc
     4261 agggccttcc atttctgcta aagtaattgt ctctccagca tttatcggaa cagaaggtga
     4321 cccttttctc ccaattccta gatgactcgc tgtcattgcc gctttccctt tttcaccatt
     4381 tttattttct ggtgaaatag ttctactttc ctctcccttg tggagccata tattatttaa
     4441 acaattcatt ttattcgtcc tctatccttt cttcactttt ttgattattc atatagatga
     4501 tttgttgttg tgcctccgca ataattgctc caatatctaa attatcattt cctaactgca
     4561 aagcgattga cattgccaag ggaagattca ttcctgcaat tacgtgcact ctgtcagaaa
     4621 tataggtaaa aaacttttga tttacacttc ctccagccat gtcagttaaa acaattattt
     4681 cgtctgtagt atccactaac tctttaattt gttcatctat atcatcaatt tcttcgacat
     4741 atgcacataa gctactaatt tgattatgca tacctgttaa aaactctagt gtctctttaa
     4801 aaccctcagc tagtattgaa tggctagcaa tgatcaatct tctcataatt caaatccttt
     4861 tcctttacgc taatattcca aatgcagcca taaccatcgc aaaaacaata acgagtagaa
     4921 tcaaaatggt catactgatt tttttcttac tgagtagata atatgctgca gcagtaataa
     4981 ttactggaat taatgttggt aaaattttgt ccaacattgg ttggattttc atactaactt
     5041 caccaaaagt gaatttaagt ggcgttgaaa cactgataac tgaagcaatt aatgatccaa
     5101 ctactgttag ccccaatact gaagcagctt cagttaaata attaatcttt tcaccgtatt
     5161 cagtaacaat cttcattccc tgcttataac caaattcaaa gcctctcatt cggaaaaaga
     5221 agattaccat gattaaaatg aaccataaaa agattcctac aggattattt tctaaagcca
     5281 tataagccgc aattgatccc atgatggttg ggatcatcgt catcaaaaga gagtccccaa
     5341 taccagcaat tggacccatc gtaccaactt taaagtcatt aatcgcatct tttgcttcta
     5401 atccttgctt ttcctccatt gcaacacaag cacctaaaat aatggctgcc agccaaggct
     5461 gtgtattgta atactgaaag tgattattta aagcttcgat gtattcatca tcatctttgt
     5521 ataattttct taaaataggt gacaatgcgt aagtcacaga tgcaccttgc tgtgtttgat
     5581 aattaaaggt acaaactgac atcaaccaac gatagttcgc tttcctaaga tcctttttcg
     5641 ttacttgata tttttcgctc attttattca tcctccccaa taaacccaac atcagaagct
     5701 cccgcagaag cagtgatttg tgcatctttt tcagctctat gatacacatt aaatgctaaa
     5761 gcgaatccta tgatcgccgc tccaataata ggtaccttca aaaaagcggc taagaaaaat
     5821 ccaacaagta aataactaat gtatcgacct gtaggcataa agcgtaacaa catcgcaata
     5881 ccaacagcag gcaacatttt tccggctaat gttagcccat ccataaacca attaggtaaa
     5941 acatccaaaa cgtaattaac agcagactgt cctaaagtaa cggcaataaa tactggaatt
     6001 gctgcagata aaccaaagaa aattggcccc aaccacaaaa cactattcat tttcttaaat
     6061 tctcgtttat tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa gattttgaca
     6121 attacatcta gttgcacccc taacatacct acaggtagtc ctattgctaa tccggcttct
     6181 actccaagct ggctaccaaa tgccgttgca ataattgcgc acgttccata gtccggtaca
     6241 gatgagccac caaaaccggc aactcctaat gacatcaatt gaactgtacc gccaactatc
     6301 aaacctgttt ttatatcacc catcaaaatt cctgtgaata atccccaaaa aactgcgtaa
     6361 tttacaaaga tcatcggaat cccataataa tcaacatgtt taataaatgc taatatagtc
     6421 agcataataa tttgaaaaat agtaaaatgc attatcctta ctcctttatc ctaattttga
     6481 ttgaatttcc tgtgccttat ccgccggaac aaattgtgta acaatttcta caccagaatc
     6541 caacaatttt ttatacgtgc tgatattttc atctgatgca taggctctag aagatagctg
     6601 taatgcatta tctttaacaa cagtaccgcc aacaattagt cggtcaattc taataccgtt
     6661 atctactaaa tgaaggattg tttccggctc tttcacgatg acaaatactt tttcctgatc
     6721 gtatttcccc tttttaaaat tcgcaatcgc tgtttcttca gtgataattg atacagccat
     6781 tccagcaggt ctagctaaac gcatacttga tttttggatg tcgttatttg cgacagaatc
     6841 atcgatcacc atcactctgt ttgctttaga cattggtgcc cattgcgtga caataatccc
     6901 atgcaataaa cggttatcaa ctctcgacat aacaatactc ataaatactt cctcctttat
     6961 ctttcatttt cttacaagca attaccatgc caaaacaaaa agaacttgat aaatcaagct
     7021 ctttttacat aatcataaac gtatttaacc tcatgaatcg ggatgtcgac actataataa
     7081 tgttcaacga cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa ttcctcactc
     7141 gtttcattga aataaatttc atcttccaaa gtattttgtt ttactaatcg ttcaattaag
     7201 caactaatgt gaacatataa tccgaaacac gtattattat caaatgaaat tcctagatca
     7261 ttttgtaaga tgtatatagc atctgaaacc cgttctaata atttagtcgg atttaatatg
     7321 gtcaattgct ccattaaatt cgttagacta aaattcttca gaatattatt gttcaaaact
     7381 ttcagttcat tatcattaaa gtgtctattc aattgattgg agaatttact tatattcatt
     7441 cctaaaatca tatcttcaat tgaaaagaat tctacatctt ctagatgtgg atttagtgtt
     7501 ccaacaataa aaagaatatt gttctcttta cgaagctccg ataaatagtt ttcattcact
     7561 aaagagtaat agtcacacgt ttctatgaca agatcagtat cccttggaaa gctatcttca
     7621 agtatccttt ttaatttaaa cgaagttcct aatccgcttg cacaagaaca gacaatcata
     7681 tccttcttaa tatttgatga ttcataaata taatccacat tttgattttc ttttacttgt
     7741 tcaagaatct gtttaagcgg taaatctaat aatatctgat ttgcaacatc aatagccatt
     7801 cttgttgaaa cattgtttag tagtccgaat tttttttgag cgtctagttc caaatttaga
     7861 taaattttct ccagtgaacc catatcaaca agtaaaataa tttcttcata tccggtacac
     7921 tcttgtaagt atctattaat tttgtctgca atctcactac ttgaagtcat tagtggcata
     7981 tcaatggcgt cgtatatata tctctcaacg atcctattag cagtacttgc tatacaggtt
     8041 gccgttagat ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa ttctttacta
     8101 cacattaact taaataaaat aaccaaaaca tttttttgaa tttttgttaa ataaatatcc
     8161 cctatttgaa taaaacgatt gactaattga acttctcgac tacaccaact attaatatca
     8221 ttgatgaagg aatataattt atcgtccgat gttaattgga tttgataaat ccaagaattc
     8281 aaaaagatag acgttatttg aagataataa tagtcaaatt ctatttgaaa atctctctct
     8341 atttccttaa catactgtct aaatcgaatc tcttgatagg tgggatatgt taaagctgaa
     8401 ttattttgga tatcaacatt cttttgtcta aaatatctat catatttctc aaaaagctgc
     8461 tgcttatcag tgtctttctt ccacgtttga taaagatact ctataagttc gttgatatta
     8521 aaaacttcgt ctactttttc caaatcatcc atttgaatat attttaattc attctgtctt
     8581 ttcataacct tcatgccgtt gaaattcacc cattgtggta aatgatacat ctgaatataa
     8641 agttcttcca aatcatctaa gaaataaaag gcattggcac aggtcaattg aatgatattt
     8701 ttcaaattgc caacattttc actaaagcga tagtttaata aaaaatctaa taattgttga
     8761 gatatcagta ttttctttgc tagtctttct tcttctgatt taaaaaatat cttgattaac
     8821 tgttttcttt cataaagaga acgactgtct aatgttggaa gtgttagctt tacaggtatt
     8881 cttctcaaca aagttgataa tagtttatcc tcaggtggtt ctgatgttgc aaatataatc
     8941 cagcaacttg atgtgtacca gttttcatta tcacccatcc gatgaaattt cctcgtatcc
     9001 ataaactgaa aaagtttctc ctgacattca ttgctcaatg catgaatttc atctaaaaat
     9061 aagactccat tattagctaa ttctgctaaa ccctttcttc catcttcggc tcctgtatat
     9121 gcacctttta catgcccaaa aaaattggac aagaaaagtt ctggattatt ggcatattca
     9181 ctacaattaa ctgaaatgaa agatgccttc tcttctatat aattattggc caacaaatcc
     9241 ttatacacat aatttgctaa atagcttttt ccagttccag tctcgccatg aagtaaaatg
     9301 ggtaagccat ttggcggata gtagattgct gctttaattt gagcaattac atttcgcaga
     9361 cttccttgac ttccgataac cctttctatt ccaaccctaa aagagctatc ataccatttt
     9421 tccgcttcct ctgaagagct aaaaatttgt tgatttggaa ctttctccgg taaagaaaag
     9481 aagattactg gacgagtatt gatttttcca aattctccat cttttacgaa ttcattcaaa
     9541 tattgagaga caatattacg actaacattt aatttttcag atattttctg tgctgtattg
     9601 ttcccccatt tatttttaat ctcagagttc tctatgacat aattgacaat gcgttctttt
     9661 aattcttcca tcacagcacc tcctcgacac tattcgaatt ttgccgacag tatttttcca
     9721 taaatatgac ttgctaaaaa ggctttgttt agatcattca attggaacgt tgcttgtttt
     9781 gcttgatccg ctaagacgtt gaaaagatac tctggcgaaa tcgcataccc caaaatatca
     9841 acgattgttg caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac
     9901 atcgtttgtt tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact
     9961 tgttccaaca aacggtcgga taaatattta tagaaaatca gccccaataa ataattttta
    10021 tattctgacg catccatttt actacggaga ttatctgccg cactaaataa cttctgattt
    10081 aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt
    10141 attttagcat actcagtttt agaaaaagca aaaaaataaa gaaacttatt cttgataaca
    10201 agctacgtga cgtatatccc taacacaaaa atagccatat cctttggttt ggatacggct
    10261 atttgatatt actcattcaa ttatcaaatc acagtaggtg tgaatctcat cgggatcggc
    10321 aatatctact tctgattagg atcaaaggtg attttctcaa taaaggtgtg atacaaagct
    10381 ttaacttgct tcttctcact ctggataaga aagcaatcca aactagtcaa tatctgttgg
    10441 acatctttga cttgaatcgg ttccccttgt tgttgaaaga tccctaagat ttcattctct
    10501 cgaatttgta actgtcttcg tttttcagtc aattcattta aacgatcttt taacatcgga
    10561 aacaattcgg gactatcttc caaagcagct tcctgttttt gctctaatag ttagattttg
    10621 cgtaccattt tcttctttta ttttccttcg attgctgatt ttaatatagt ctaaaggaaa
    10681 tttatattta t
//
LOCUS       DAYYCQ010000090        10649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000090 DAYYCQ010000000
VERSION     DAYYCQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.091"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACHAYF_002427"
     CDS             23..646
                     /locus_tag="ACHAYF_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579812.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACHAYF_002428"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACHAYF_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8579813.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACHAYF_002429"
     CDS             2142..3023
                     /locus_tag="ACHAYF_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8579814.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACHAYF_002430"
     CDS             3026..3796
                     /locus_tag="ACHAYF_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579815.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACHAYF_002431"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACHAYF_002431"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8579816.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACHAYF_002432"
     CDS             5107..6270
                     /locus_tag="ACHAYF_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8579817.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACHAYF_002433"
     CDS             6387..6797
                     /locus_tag="ACHAYF_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8579818.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACHAYF_002434"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACHAYF_002434"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8579819.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACHAYF_002435"
     CDS             9370..9654
                     /locus_tag="ACHAYF_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579820.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACHAYF_002436"
     CDS             9814..10050
                     /locus_tag="ACHAYF_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8579821.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ACHAYF_002437"
     CDS             10047..10445
                     /locus_tag="ACHAYF_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8579822.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3717 a         1773 c         2253 g         2906 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatata
//
LOCUS       DAYYCQ010000091        10082 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000091 DAYYCQ010000000
VERSION     DAYYCQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.093"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..2229)
                     /locus_tag="ACHAYF_002438"
     CDS             complement(109..2229)
                     /locus_tag="ACHAYF_002438"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8579823.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="ACHAYF_002439"
     CDS             2459..3637
                     /locus_tag="ACHAYF_002439"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8579824.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4390
                     /gene="aroD"
                     /locus_tag="ACHAYF_002440"
     CDS             3653..4390
                     /gene="aroD"
                     /locus_tag="ACHAYF_002440"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8579825.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQII"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="ACHAYF_002441"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="ACHAYF_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8579826.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="ACHAYF_002442"
     CDS             complement(4990..6243)
                     /locus_tag="ACHAYF_002442"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8579827.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="ACHAYF_002443"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="ACHAYF_002443"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8579828.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFISGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="ACHAYF_002444"
     CDS             7821..9167
                     /locus_tag="ACHAYF_002444"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8579829.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="ACHAYF_002445"
     CDS             complement(9475..9861)
                     /locus_tag="ACHAYF_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8579830.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10082)
                     /locus_tag="ACHAYF_002446"
     CDS             complement(9910..>10082)
                     /locus_tag="ACHAYF_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8579831.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         3215 a         1838 c         2053 g         2976 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattatttaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt atttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tg
//
LOCUS       DAYYCQ010000092        10031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000092 DAYYCQ010000000
VERSION     DAYYCQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.094"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(495..719)
                     /locus_tag="ACHAYF_002447"
     CDS             complement(495..719)
                     /locus_tag="ACHAYF_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579832.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            910..3687
                     /locus_tag="ACHAYF_002448"
     CDS             910..3687
                     /locus_tag="ACHAYF_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8579833.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3768..4187
                     /locus_tag="ACHAYF_002449"
     CDS             3768..4187
                     /locus_tag="ACHAYF_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8579834.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4199..4669
                     /locus_tag="ACHAYF_002450"
     CDS             4199..4669
                     /locus_tag="ACHAYF_002450"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8579835.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4686..5465
                     /locus_tag="ACHAYF_002451"
     CDS             4686..5465
                     /locus_tag="ACHAYF_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8579836.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5452..6273
                     /locus_tag="ACHAYF_002452"
     CDS             5452..6273
                     /locus_tag="ACHAYF_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8579837.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6290..7339
                     /locus_tag="ACHAYF_002453"
     CDS             6290..7339
                     /locus_tag="ACHAYF_002453"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8579838.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7352..8356
                     /locus_tag="ACHAYF_002454"
     CDS             7352..8356
                     /locus_tag="ACHAYF_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8579839.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8989..10008
                     /locus_tag="ACHAYF_002455"
     CDS             8989..10008
                     /locus_tag="ACHAYF_002455"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8579840.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1654 c         2094 g         2921 t
ORIGIN      
        1 cttctttttt aatttgttct tgcaatcttt tttgctacgt aatttttgta aaaaagtggg
       61 ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa aatgagaaaa atatttattc
      121 gtttattgtg ggtactatct tagaggaata aataatagtg gagaaaacta tggtttggcg
      181 cagattctat caggtttgat ggaacgaaga caacctcccg acatcaagtc aaaagccact
      241 tgagattttg atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat gtttctacct
      301 tgttttcgta atcaatggga aaaaactaag agtggtgtac gtaatcaata aaaaggatgg
      361 tgacaacagt acctctttac tctctgaaag catcataact cgtttttcct cattatgcca
      421 ataaattgtt gttataccac atataaggat ttgtaatagc taaaaacaga caggaaagtt
      481 gaaatctgtg aatcttattt tgtatggaag gatttataga acgcaaaaat caagataaag
      541 atgaataaaa atcctgtttc ggctatatat tgcgtatcta cgccaataaa acgagtttgt
      601 tctgtcatag ccatataaag caaaggcaaa aatacaacta cagcgagata agatccgatg
      661 atttctcgaa aagttttcca ggaagtatgt ttcctgtttt tatatttatc cgatttcatt
      721 gaatagtcat agcctcctta ttctttcata tgttgaaatt ccagtataaa aactggaagg
      781 aactgtacgt tttttagaat aaaaattata agggataagt aggctgctgt ctataataag
      841 atatattttt caattcttta ttaaagattt tattgacaaa atgaatccgt tttctatgat
      901 ggaggagggg tgaataagtt gttgaagaag gaaattttat gttacttaga gaaccagaca
      961 gcctttttcg atttagaaca tatgaacgag gtctttacag cacaaaggtt agctgatcat
     1021 tttggggtaa aaagaaacac agtcagtcat tatttgaacc agttaaatga tgagcaacta
     1081 ctagtcaaaa tcaattctcg accagtgatt tatttccaca aagaagcttt tgaaaaacaa
     1141 ttttttaagt tacgcacgaa tttttatttt tctatcgatg atctcaaaga ggaacagcct
     1201 tttttcgcgc aaccaaaaga cttattctct tttatgatcg gatacgacgc cagtttgaaa
     1261 gaaagtatcg aacaaatcaa aacagctctt tactatccag atggcggatt accacttttg
     1321 attacaggcg aaagtggcac aggtaaaagt tatttagtta atttggtcta tcagtattgt
     1381 ctgcttcatg atctgctaga agattctgca ccattcatca cttttaattg tgcacaatat
     1441 gcagataatc ctgaactttt gactagcaat ttgtttggac atgttaaggg agcgtacact
     1501 ggtgcagaag aaaataaaaa aggggctttt gaagcagcag atggtgggat cttgttttta
     1561 gacgaggtac atcgcttaac accagaaggt caggaaaaac tgttcaccta tcttgatcaa
     1621 ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac acgtctcttt
     1681 tttgctacga ctgaagagat tactagtcat ttcttaacaa ctttcattcg aagaatacct
     1741 atccgaatcg aactacctgc acttaatcag cgtagtcgta atgaacggct agaactgatt
     1801 tactcttttt ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt cagtggacca
     1861 gctttgtcac ttttaacaag tcagaaatta tccggaaaca taggagagct gaaaaatatc
     1921 gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg aacagcctga aatccatgtc
     1981 acgatccatc atttgcaaaa agaactactt gcgcagccta ttactaaaaa tacagcacag
     2041 caaggaatct atattacgca agaccagacc ttagagcaat taatggagaa gcaacagccc
     2101 gaacagcagc gaatcgttaa aagttttgag caaatcctaa ttgatttcaa gaaaaataac
     2161 tgtctgctat attcttgcga aggaaaatta aagcaagaag tgattcaatt atttgatttt
     2221 ttgctgtttg agaccagtag acaagaaaaa cacgagctgc tggtttattg gacacaatct
     2281 atccgagaga cctttcgaca aatggaaact gcctatcaaa tcaaattcga tggaaacagt
     2341 gtctatgcct tgagttacta tctctatcag cgaagcacta tcaaatggtt accggaagaa
     2401 catgatatta tccaactaat caaagaatta gaacaacaag tttctcagtc ttatccatct
     2461 agttatcact atgtccaacg aattctggaa ttaagtaaga tcaaattaga tattgaagac
     2521 acaagtatgg atcggatttt actgacgatc tatctaaaaa aagcgaaatg gtcaaaagaa
     2581 aaacgactgc caaaagcgat gattgctgct cacggttatg caactgctag cagtatggca
     2641 aatgtagtaa atcgtttatt acaagaggat ctatttgaat catttgatat gccactagat
     2701 gtcacaccac agcagattgc ttcagaaatc cttgattatt gcgaaaacag cgatgtttca
     2761 aatggattga tcatattagt agacatggga tctctgaaag aaatccatca gttttttaaa
     2821 aaacagttat ccgtaccgct tttgattttg aataatgtga cgacaccttt agcaatcaca
     2881 gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga gactgttcga
     2941 cagatccagc cagaatggcg actgctctat ccagaggaaa acaaacccaa agcactcatt
     3001 actacttgct tcactggaat cggtacggcg gtccacttaa gtgaactgct agaaaaaagc
     3061 ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt aaaagataag
     3121 aaaaacagtg acccattatt ttccatatac gaagtcgtag gaatgattgg caccacagat
     3181 cctgatatca ccaatatcga ctatctttca ttagaagacc tcatctcggg ggaaaagatg
     3241 tctgtactag ctgaatggtt ggacactact atgaatgctt cagagaagga aatattcaat
     3301 cagcgcatca ttcgtaattt ttcattggaa aaagtactgg attccgtgac tattctagat
     3361 acagagaagg tgatgggaga aatcgatcat tttatgagag aattagaggt gcagttgaat
     3421 cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg tctcattgaa
     3481 cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg gaagcagtgt
     3541 caaaaagaag ggttatctgc tattaaatct gcgtttagtg tcattgaaga gcattatagt
     3601 gtcatgatcc ccaattctga gattgcctat atctatgata ttttgagtga agatacagag
     3661 tttctctcga tagaagaaga attttgaaca taagcaagtt gttgccacta attttggcat
     3721 gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg gatagagaaa
     3781 ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta gagctgatct
     3841 atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat tttgatttag
     3901 cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt gttctgacgg
     3961 atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg gatcatgtct
     4021 atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg ttggacgctg
     4081 aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa atgatccagt
     4141 tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag gaagaaaaat
     4201 gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt tttcatggac
     4261 acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta cgaatgagat
     4321 acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga tcaaatcaat
     4381 tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt tattcatcgt
     4441 agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa tcaaaaccat
     4501 caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag cggtaaatat
     4561 tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag aagtagaaat
     4621 ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag aaaggaagaa
     4681 cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca caatcagaat
     4741 atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt acaggaatcg
     4801 tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta gcatttatcg
     4861 gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg attttaggga
     4921 cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta ggtttgccga
     4981 ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt ccaattatga
     5041 accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag catatgcact
     5101 tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt tcttatatgg
     5161 tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt caaaaggggt
     5221 tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct cgtttgctga
     5281 tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct tacttagaga
     5341 ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta gtcaatatta
     5401 tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa gatgaagaat
     5461 tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt ttggcgttct
     5521 tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta tgtttatgca
     5581 atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc agctttgaaa
     5641 cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt aggaatcacg
     5701 gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc gatcgatagt
     5761 atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt ttggggaact
     5821 ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa tattttaggc
     5881 cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta tctagcaaca
     5941 ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa cggtatgatg
     6001 gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc aatgactgcc
     6061 acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc tgtgactgtt
     6121 caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt tggcatagtg
     6181 ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact agctttcttt
     6241 ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa tgtttacgat
     6301 gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa agaatgattt
     6361 ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa cgggttcatc
     6421 ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc ttactgtcga
     6481 tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa tcgatactgt
     6541 gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa aaatgataca
     6601 aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac ttgctttaga
     6661 agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg ttacaaaagg
     6721 ctattctgcg actgttttac agttactact cttagggata ggaattggaa taagtaaaaa
     6781 taaaatcagc aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa taatcaacca
     6841 tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa gtttatttcg
     6901 tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag ctaaagaagc
     6961 agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt tagaagctta
     7021 tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta ttgaagggga
     7081 aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt acgttggaga
     7141 gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat tagcaataga
     7201 atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat tcgcttataa
     7261 aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact ttgatacagt
     7321 cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag aagaacaaat
     7381 caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca tcgataaact
     7441 ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt atgcttcctc
     7501 gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg tacagcatgc
     7561 tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg ttattttgtc
     7621 ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca agaaagtagg
     7681 agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc tatgcgattt
     7741 tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg cagcagatcg
     7801 attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac agctcgaacg
     7861 ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg gcttatcatt
     7921 tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga atcaatgggg
     7981 agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc catcgaaatc
     8041 tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa caaccccagt
     8101 cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg ctaaactttt
     8161 gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag tagaaggtat
     8221 cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag tgacacaacg
     8281 catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc gctactatcg
     8341 tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag agagcagcga
     8401 taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa acacattttt
     8461 tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg ccaaccattt
     8521 tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag tccaagtaca
     8581 acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa acaaaagttc
     8641 ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga tttagaaatg
     8701 ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt cctaacctcc
     8761 taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac aagcaaagca
     8821 taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat ttcaagtaaa
     8881 aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca actttaagaa
     8941 atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat gaaacaaaaa
     9001 atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt ggcagaaaac
     9061 gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat caataagtat
     9121 catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat cggatataaa
     9181 gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc aacaaaagcg
     9241 attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat cattatacat
     9301 gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt ggctgaagaa
     9361 attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca tgctgaggaa
     9421 gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa agcagcagcc
     9481 tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa tgatgtgatt
     9541 ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg cgctatccat
     9601 ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt ggcagaaatc
     9661 agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt gagtggtgtg
     9721 ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc cggtaacttg
     9781 ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt cgttgagggt
     9841 gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga gatgccaatt
     9901 actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc agcaaaagaa
     9961 atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat agatgatcaa
    10021 tagttaatcg t
//
LOCUS       DAYYCQ010000093         9948 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000093 DAYYCQ010000000
VERSION     DAYYCQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.095"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3358
                     /gene="fss3"
                     /locus_tag="ACHAYF_002456"
     CDS             131..3358
                     /gene="fss3"
                     /locus_tag="ACHAYF_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8579841.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3471..3785
                     /locus_tag="ACHAYF_002457"
     CDS             3471..3785
                     /locus_tag="ACHAYF_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8579842.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3798..4172
                     /locus_tag="ACHAYF_002458"
     CDS             3798..4172
                     /locus_tag="ACHAYF_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8579843.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4182..4517
                     /locus_tag="ACHAYF_002459"
     CDS             4182..4517
                     /locus_tag="ACHAYF_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8579844.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4600..5940
                     /locus_tag="ACHAYF_002460"
     CDS             4600..5940
                     /locus_tag="ACHAYF_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8579845.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6018..6692
                     /locus_tag="ACHAYF_002461"
     CDS             6018..6692
                     /locus_tag="ACHAYF_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8579846.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6685..6849
                     /locus_tag="ACHAYF_002462"
     CDS             6685..6849
                     /locus_tag="ACHAYF_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579847.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6925..8067
                     /gene="dcm"
                     /locus_tag="ACHAYF_002463"
                     /pseudo
     CDS             6925..8067
                     /gene="dcm"
                     /locus_tag="ACHAYF_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8057..8347
                     /locus_tag="ACHAYF_002464"
     CDS             8057..8347
                     /locus_tag="ACHAYF_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579848.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8606..9790
                     /gene="mobT"
                     /locus_tag="ACHAYF_002465"
     CDS             8606..9790
                     /gene="mobT"
                     /locus_tag="ACHAYF_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8579849.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9787..9924
                     /locus_tag="ACHAYF_002466"
     CDS             9787..9924
                     /locus_tag="ACHAYF_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8579850.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3294 a         1679 c         2247 g         2728 t
ORIGIN      
        1 aaatttttag gctcattcat tctcaaatcc attctattca gtcaataaaa attttaaaaa
       61 gactttataa acaaagatgt tcaagcgagc atcttatttt tttgtgctta atttgaaagg
      121 agaacatttc atgtataaaa aacaaaaaaa gaaattatgt gtattaacta ttttgagttt
      181 ggctgttcaa gcaattgttg gagcaattgc tcctacgatt acttttgctg atgaaattac
      241 ccatccacaa acggtaaccg tagaactaga tttagcacat caatatgctg ttgaaggaac
      301 ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg cattatgcag tgtacaacgg
      361 ctttaaacaa gatattttct gtattgaacc aggagtcccg atttacaatg agttcactcc
      421 tggctatgag aaaaacccgt taccagatat gtccgataaa gccaaattag tttccgtttt
      481 atggaaaaaa gtaggtacag atgcagatac tcatattgtg gcacagaaga tgatctggca
      541 agaagttaat gggtataccc ttcactcaat aaaacgatca gatggtagtg ccgtaaatat
      601 cgcagccatt gaggcaaaaa taaatcaagc cattgacgat tatcagaaga aaccaacctt
      661 ccataatagt acggctaaaa cagtattagg gcaatcgact actgtgacgg atacgaataa
      721 tctgaatttg tcagagttcg atgaggttgt ggaaaatgcc gcaaacattg attatcgtgt
      781 gaatggtaat cagttgataa tcaccccaaa tgccaattca aaagaaagtg gtgtgctgac
      841 gcttaagaaa tctgctggta ccggaacacc tgtcgcctat aaaaaggctg gccaacagac
      901 tttaatggcc ggggcgattg ataaacctaa tacctacaca atcaaaattg atgtggaaac
      961 ggagggttct ttaaagatca aaaaagtcga taaagaatcg ggtgatgttg taccaggaac
     1021 ggttttccat ttagactttg gaaaaacttt acctgcaaaa gacgtgacta ctgacaaaga
     1081 aggcattgct acattggatg agattcctca tggtacaaaa gtaaccatta ccgaaaaatc
     1141 agtaccggcg ccttatacga ttgataccac ccccatgact accaccatta aagcaggcga
     1201 aaccatttac gtgacttcaa aaaacgcacg ggaaaaaggg caaatcattc tggataaaag
     1261 cggagttgaa acagggagtg atctttggaa tgacaattat tccttagctg gtaatacgtt
     1321 tgccattcgc aaagacagtc caaccggtga aatcgttcaa gaaatgacca cggatgaaaa
     1381 cggtcatgcg gaaacaccaa aagagattgc taatgcgttg gaattgggaa cctattatgt
     1441 gaccgaaact aaagctagtc atgggttcgt gaatacgttc aaacctgtaa aagtcgaatt
     1501 gaagtatgcc aatcaaaccg tagcccctgt taccagtaac gtaaaagggc aaaatcaaga
     1561 agtcactggc gaaaccactt tgacgaaaga agacaaagat actggggata aagcacaagg
     1621 taaggcagtc tttgagggtg ctgaatacac cttgtttact gctaaggatg gaaaagctgt
     1681 taaatggagt gaggctttta aaccagagtt agcgaaggga acgaaaggtt ctgatgaaac
     1741 ggtcactttg actttagacg aaaagaacca agttgccgtt aaacatttag ctatcaacga
     1801 gtattattgg caagaaacaa aagcgcccga aggatatagc cttgatgaaa caaagtatcc
     1861 tgtatccatc aaaaaggtgg atgatagcga aaaaaatgcc gtgattaccc gaaatgttac
     1921 ggcaaaagaa caagtgattc gctttggctt tgatttcttt aaatttgctg gatcagctgc
     1981 gggcactacc gaaactggct tcaatgatct gacctttaaa gtgtcgccat tggaagggac
     2041 caatgaaatc acaggtgccg aagatgaagc gaccacagct tataacgagc aattagggtt
     2101 tgatggttat ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga
     2161 agccccagaa ggttttcaaa agattacgcc attagaaatc cgttctactt ttaaggaaaa
     2221 caaagaagac tttgtgaaga gtgagtatgt ctttaccatt accgaacaag accaaaaaca
     2281 accaattaaa acggtgaccg ttccttacga gaagctgacg aacaaagcat tttctgttag
     2341 tttgaaccgt ttaatgctct atgatttgcc tgaggaagaa gatagtttaa cttctcttgc
     2401 gacttggaaa gacggaaaca aagaattaac ttctctggat tctaccgagc tagttgataa
     2461 attgagttac aatctacatg aaatcaaaga agactggtat gtggtagcgc aagccattga
     2521 tgtggatgcg acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtgattgc
     2581 agaaacaagc gcaacgttgg cgaacaaaga gaaaacagga acttggaaaa ttcagcataa
     2641 attaaccgcc gaacaagttt taaataaaac tattgtctta ttcaactatg tgtatgaaaa
     2701 taaggaagct tttgaagcag gcgataagcc agtagcgaag gatgttagct tgaataacca
     2761 agcccaaact gtcagttgta cagtggaaca ccatgtttct atccaaacaa aggcccacct
     2821 agaaaacggt tctcagacct ttactcatgg tgatgtggtg gatatgtttg atgatgtgtc
     2881 gattactcat gatgtactgg atggctcaaa agaagctttc gaaacaattc tgtatgcttt
     2941 attaccggat ggtacaaaca aagaaatttg gaaatctggc aagattgatt atgaagtaaa
     3001 tgataaagaa ttcaccaaaa ctgtgcttgc gaaaaaagta gataccggaa aatatccaga
     3061 aggaaccaag tttacttttg cggaaatcaa ttacgataaa gatgggaata tcaatggaaa
     3121 acacaatgaa gatttgaaag aaaaatctca aaccttaaca ccaaaagaag ttccaaccat
     3181 actaagtacg ccgaaacaac cggaaacacc aactgttcca agtggctctc aagaatctag
     3241 tcccacagtg aagaccttcc ctcaaaccgg ggagaaaaat tccaatgttt tactgttcat
     3301 tggttttact ttgatttttg cgacggcggg ctattatttc tggaatcgcc ggaactaagg
     3361 tgatgagatg atacaagtaa atcctaagaa ccaacgtgcg cgaccgcctc ctaaaaaagt
     3421 agtcgcgcta tttttgtatc caaaactaac aatgaaaaga ggaaaagaag atggaattga
     3481 aatttgtcgt gccagatatg gctgaaacct ttggaaagtt aagttatgcc ggtgaaggag
     3541 aaattataac agaaggatat ggacggaata ctacggtgat tggtcgcagt tatcatttgt
     3601 attccagtaa gcaacgggcc gatgatattg aggtggtagt agctgcggaa gccggtgaaa
     3661 aggattttga tcaagaccaa ccgctaaaag ccgtgaatcc ccatttggtt gccaaaggct
     3721 atgagattga gaatcgtggg tttactgatt acgtgttgta tgttgatgat ttagtgaaag
     3781 cataggagga aaagaagatg agattaacag aaggtattgt cgtagattca ggtttaacat
     3841 ttgggaagtt gcgtttttcc gcattacgcc gagaagtacg gaagcaaaat gaggatggaa
     3901 caattggtaa tgaggtaaaa gaacgaacct ataatttaaa atcctctgca caagggcgaa
     3961 tgattcaagt tagtatccca gctaatgtac ccttacgcga gtttgcttat gatgcagagg
     4021 tagagttggt caatccaatc gtggataccg ttgccaatta tgtttttcga gaaggaacta
     4081 ccgttaattg gttcattaag gcggatgatt tagtcttgaa gcggcagccg aatcaaggaa
     4141 atcctacgaa ccaaaatgaa agaaagaaat aggtgaagat tatggaaatt aatgttgaac
     4201 atattttaga ttgtctcgat caatatggaa aaggcgagct gactgaggaa caactcgtta
     4261 aagcattaac ctctgatgaa aaaatgtttc tgattataca tcaaggttta cttggagaaa
     4321 atagcgacag tgaagaagac tctgatgtac tagactggtt aggagaagaa gagtctttct
     4381 ttatagttgt cgaagtaaat gaaaacttat gtcggcaggc agaatccatt ttggaagaaa
     4441 ttggtgtgga gatgccggat gctattgaag gctttttaaa tcaattggtt gaaacgaaac
     4501 aactcccctc ctcgtagcag taaatgacta gcataacttt ttgaaattgc ctaatgaaat
     4561 cctattgggc tttttcgatt ggaggtggaa accatcaaaa tgtacaaagg acatcgcata
     4621 cgcgcaggag atcagcactt ggtttatcac tttgttcttg gctggctact cgctctgttc
     4681 atcggttgga tgagcgtctt ttattttcaa gagtttagac aatttgatat ttctaagcta
     4741 tcactttcta ctattgaaat cgtttggtct ataaaagatt tagtatgtct gcttggaagt
     4801 ctcgcttttt caggagctat gatattgctt tatattcact tttttcttga tcattggcga
     4861 agcttgtggc atcgacaaaa gctagctcgg atgattctag agaaccattg gtatgaagtg
     4921 aaacaaactc aaagtgaagg ctttttcaaa gatttgaata gtagccgaac cagagagact
     4981 atcagttact tccccaaaat ctgttatcga atgaaagatg gattactgtc tattcgcgtt
     5041 caaatttcac ttgggaaata ccaagatcag cttttaaaat tggaaaagaa gttagaaagc
     5101 ggactgtatt gtgagttagt agaaaaggaa ctcaaagact cctatgtgga gtatacttta
     5161 ctgtatgata tgatcgccaa tcgaattggg atagacgaag tagtggcaga aaatgggact
     5221 ctgcgattga tgaaaaatca agtatgggct tatgattctt taccccatat gttaatcgct
     5281 ggtggcacgg gtggagggaa gacctatttc ctcctcacca ttatcgaagc actattgaag
     5341 tcagatgcag aattgtttat cctcgatccc aaaaatgcag atttagctga tttaggtacg
     5401 gtgatgcctc atgtttattc acaaaaggaa gaaatttctg cttgtgtgga agatttctat
     5461 gaacgtatga tggctcgtag caaggcaatg aaagaaatgt ctaactacaa aacaggagag
     5521 aattatgcct atcttggact tccacccaac tttttaatct ttgatgaata cgtggcttat
     5581 atggagatgt taacgacgaa agaaagtgcg gtgattttga ataagctcaa acaaatcgtg
     5641 atgttgggtc gtcaatctgg tttctttctg attttggctt gccaaaggcc ggatgcgaaa
     5701 tatttaggag acggaatccg cgatcaattc aactttcgcg tggctttggg tcgtatgagt
     5761 gaactcggat attccatgat gtttggggag gtcgaaaaaa acttctttat gaagcacatc
     5821 aaaggtcgag gttatgtgga tacaggtggc agtgtgatta gtgagtttta tacgccactt
     5881 gtaccaaaag ggtatgattt cttagattca attagtaaat tagaaaagat gaatgattga
     5941 gtgatcttca atactgactt tatacttcaa gtaacgtata atacttttat atagtaggaa
     6001 gaaaggggat aattaatatg gaaagtaatg attcttattt agagtatgca aaagcaaata
     6061 aggataagtt tatttcttat attatcaaag ggaaatcccc cgaagttgga cgagacgcag
     6121 tttttatggc agggagtcct ggggctggaa aatcagaggt tgccattgga ttagctgaga
     6181 attatgataa ccatgtgatt attgatgcgg attactttcg cacccagttt cctgattata
     6241 atggaaaaaa ctctagtctc tttcaaaaag catcttcttg gttggtagag caggcattta
     6301 gatatgtctt agatcatggc tattctttta ttctggatgc aacttttgca atattaagtg
     6361 ctgaaaaaaa tatctcgcgg gcgttgaaaa aacattttcg agtgacgatt ttctatgttt
     6421 atcaagaccc taaaatcgct tggaaattta cacaagaacg tgaaattgca gagggacgtc
     6481 atgtgccgaa aaaggcattt attaacgctt ttttcaaatc aagagagaac attgcaaaag
     6541 taaagagtaa acatccagat gtattgcttc atatcataat aaaagattat acgaataata
     6601 tttccgaagt tcattatgct acggataatc tagaattatt tttaccacta gggtatagta
     6661 ggaaagattt ggaggttcaa ttacatgact gagaaagaac aagtaacgaa aattgtaaag
     6721 aaatacaata agagcattgc ggatttatcg gaaaatgcta ctgcaaaaga gttcaaaacg
     6781 gtgattaaat atgtagccga tcaggccaat gaaaaacaac ggaaattagt tggattaaat
     6841 aaaaaatagt aggacaaatt aataatgtat gagccaagag caaaatgttc ttggcttttt
     6901 tctttgcaat gaatgaggta atgtatgaga tttttagatt tattcgccgg tattggtggc
     6961 tttcgtttag gtatggaaca ggcgagccat cattgtatcg gtttttgtga aattgataaa
     7021 tttgctagac gtagttataa agcaattcat gatacaagta aggaggtgga aatgcatgac
     7081 atcacaagtg tatcagatga gtttattcaa tccctcggac cagtggacat cctttgcggc
     7141 ggatttccgt gccaagcttt ttcaattgcg ggaaagcggc aaagattttc cgatactcga
     7201 ggtactttat tctttgaaat cgctcggttc accactcttc tccgacctaa gcttttattc
     7261 cttgagaacg tccgaggatt gctcaatcac gaaggagggg ctacgttcga gacgatcctc
     7321 cgaacgctgg atggattggg gtatgacgtg gagtggtagg tgcttaactc aaaggcctac
     7381 attccccaaa gccgggagcg gatattcctt atcggacatt ctcgagacgc atgtaccgaa
     7441 caagtatttc ctatcattgg accgtcttca acatctgatc aaaacatcag aaatttggta
     7501 aatatcaatc cttccaatcg aggaatgggt ggacaagtct atggttcaga tggcgtagcg
     7561 ccaaccttaa caggtgatga aggaatcaaa atcgcactgc cagcaaggga tggaatctct
     7621 gtagcaggac tattaccggg aaagtttgaa caaggtaatc gagtttatga agttactgga
     7681 accgccccca ccttgtcaac gaaacaaggt ggtacaaaaa tcatgattcg aaaaaatgaa
     7741 acagaatatc aagaggtgaa gccagaagac agtgtgaatc tggcttttcc taattccaca
     7801 agtcgccgag gtcgtctggg aaaacagagt gttcatacct tactaacagg agaccaacaa
     7861 gcgatcgtca cggatcagta tcaaattcgg aagctaacac ctagagagtg ttggcgactt
     7921 cagggctttc ccgattgggc ttttgatcga gattctcaag tcaattcaga tagtcagttg
     7981 tataagcaag ccggaaactc tgtaacagtt ccggtcattt ttgatattgc cagaagatta
     8041 aaagagttgg atgtacatgg tttatgatga gttgattgga gaaatttatt gggtgattgg
     8101 aaagattcag tcagacccag aattagaaga acaattgcga cggttaaaat ttgatattcg
     8161 caagaatggt gtcaaagtcg ctggcgatcc atatttaatg aatgaggaga cggatgctcg
     8221 aattgaaata aatcaagtaa ttgcagaatt tgagcggatt gcggaccaag caaaagaacc
     8281 agatattcgt caatacttgt tagaaatgaa agcagagttg gaagtaaatg gtattacaga
     8341 tgagtaacct aaaccaacac aactgactgt taggcgctga ccgcggagga ttcgcgaagc
     8401 agaaatcctc cgtggtgctg tgcctgttca tggacggcgt tagccgacca tgatgtctca
     8461 cccccgtatc taacaggggg gtacaaaaac acaaaaaata agtaagcaac cattaagaat
     8521 gcttgttgta tcaaagatag taagtaattt gtataggtgt taactcattt gaaaaagtta
     8581 acaccttgaa tttggaggtg agaacttggc acaacggaat ttagattatc ggctattaaa
     8641 agaccgacgc aacgaatacg gcgtttcaca aaataaattg gctacggctt gtggacttag
     8701 tcggccgtac ttaaatcaga tagaaaatgg tggcgtaacc gcatctataa aaaccatgag
     8761 gaaaattttt gaccaactgg aaagctttaa tcccgatttg cctttgacgt tactgtttga
     8821 ctatgtaagg attcgctttc ccacaacgga tgcacgaaaa attattcaag aaattctcca
     8881 tttgaaattt gagtatatgc tccatgaaga ttatgccttt tactcctatc aagaacagta
     8941 tgtcatgggt gatattgtgg tgatgttgtc tcatgaagaa gataaaggtg tgcttttaga
     9001 attaaagggg cgaggttgcc gacaatttga aactttttta ctcgctcaaa agcgtagctg
     9061 gtacgacttt ttcgaggatt gtctaaaggc cggtggcgtg atgaaacgct tggacttggc
     9121 aatcaatgat cgagtggggc tattggatat acctgagcta acgaaaaaat gtcagaagga
     9181 agaatgtatc tccttgtttc gtacctttaa aagttatcgt tccggggaac ttttaaaggc
     9241 tgatgaaaag gacggcatgg gcaatacctt atatatcggc agtctcaaga gtgaggttta
     9301 tttctgtttg tatgaaaaag actatgaaca atatatcaag ttagggattc cattagacca
     9361 aactgagacg aaaaatcgtt ttgagattcg actgaaaaat gaccgtgctt ttcatgcgat
     9421 tcaagatttg ttaaaaggtc gtagcattga gagcaccacg ttttccatta tcaatcgtta
     9481 cttgcgattt gcggataaag tagaaggtaa aaggcgaacc aactggccct tgaatgaaca
     9541 atgggggcgt tttattggac ggaatcggaa agagattcaa ttaacttccg agccaaaacc
     9601 gtataccatt gaacgaacct tgaattggct aggccgacaa gtcgccccta cgtggaaaat
     9661 ggctaaggag ttggatcgat tgaatcggac aacctatatc caggatatgg tacagaatgc
     9721 acgcttatca gaccgacata aaaagatttt ggagcaacaa agtatggcaa ttgaaaattt
     9781 gattatatga aaagaggaat cgtcatggga attgtaaaag atattttttt ggtatttggt
     9841 ggcagtatgc ttggtgttac tgtgatgtgc ttgatgaatg caagtgcagc agcggatcga
     9901 gcaatggaaa agaaaggaaa gtgacaagat gaatttcgga caaaattt
//
LOCUS       DAYYCQ010000094         9798 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000094 DAYYCQ010000000
VERSION     DAYYCQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.096"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..807
                     /locus_tag="ACHAYF_002467"
     CDS             115..807
                     /locus_tag="ACHAYF_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8579851.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
     gene            1235..1627
                     /locus_tag="ACHAYF_002468"
     CDS             1235..1627
                     /locus_tag="ACHAYF_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579852.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(1800..2003)
                     /locus_tag="ACHAYF_002469"
                     /pseudo
     CDS             complement(1800..2003)
                     /locus_tag="ACHAYF_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1969..2166
                     /locus_tag="ACHAYF_002470"
     CDS             1969..2166
                     /locus_tag="ACHAYF_002470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579853.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(2209..2784)
                     /locus_tag="ACHAYF_002471"
     CDS             complement(2209..2784)
                     /locus_tag="ACHAYF_002471"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8579854.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(2939..3465)
                     /locus_tag="ACHAYF_002472"
                     /pseudo
     CDS             complement(2939..3465)
                     /locus_tag="ACHAYF_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3922..5433
                     /gene="gadC"
                     /locus_tag="ACHAYF_002473"
     CDS             3922..5433
                     /gene="gadC"
                     /locus_tag="ACHAYF_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM8579855.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            5446..6846
                     /locus_tag="ACHAYF_002474"
     CDS             5446..6846
                     /locus_tag="ACHAYF_002474"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM8579856.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(7011..8446)
                     /locus_tag="ACHAYF_002475"
                     /pseudo
     CDS             complement(7011..8446)
                     /locus_tag="ACHAYF_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            8839..9432
                     /locus_tag="ACHAYF_002476"
     CDS             8839..9432
                     /locus_tag="ACHAYF_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM8579857.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
     gene            complement(9416..>9798)
                     /locus_tag="ACHAYF_002477"
     CDS             complement(9416..>9798)
                     /locus_tag="ACHAYF_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8579858.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         3251 a         1571 c         1731 g         3245 t
ORIGIN      
        1 atccttttta aataattttt tagacaaaaa acagaaagtg tctaaagaaa gcttgacgaa
       61 gaaaataatt tttaataact tatttaagga aattttttga aggagaagat aaaagtgaga
      121 aaaaagatta ttattaatgc tggagtaatt ataggattga tgttagtaat tttctccgga
      181 aagcaatatg ctgataatat tttattatgg ggaggggaag ataatattga aaagatcaac
      241 aacaatttaa ctatattaga cacagctcta gaaaataaag agcaaaaaat tagtatttta
      301 aattcacaat tgtcttcaaa caaccaaact cttgaacaac taagaactaa tattgaagtt
      361 tccaaacaaa aagtggcatc tttagaaaac gacaagaatc aattagtatc agagaaaact
      421 aatttagaaa atcaattgtc agcaaaagac tctgaattgc aaggaaaaca aaatgaaatt
      481 aatgcgaaaa tagacgaaat taaccaaaaa gtagctgaaa taaatcgatt aaatagcgat
      541 tggaataatc gtttacaaaa tgaaaagaac aaatttaatg aagaacaatc tactattaat
      601 agtttaaacc agcaaattaa tcaaatgttg agtgaaaaaa ataacattca aaatcaactg
      661 caagctactc agcaaaagct agaagaagaa aagtcaaaaa atagtagttt gaataattac
      721 attgaaaagc ttcaaaaagc taaaaatgat gtagaggata ccgcaaataa agcacaacaa
      781 attgtaaatg aacatacaat ccaatagata tagttttaat ttaacaaatg gtatgtttgg
      841 agataatgag atagaatata aatgtcataa tattatacaa tctaaacacc gtttcctttt
      901 ctatttttga tgacatcagt atatagctac tcgaaagagt agtcttttta ttattctcgc
      961 ttattattga tatgaaaatt tcgatttgac aaaaggtacc gaataatttt gtaatatgaa
     1021 ttattatcaa gaagtaatta tgatgataac atatggaaac tagttagtaa taataacagg
     1081 tgattaaact tttgaacgac ttgtaattct atattttttc ttttaagtaa aaaatatagg
     1141 tgattttttt tgacttcata tttataataa agacacgtaa atcaattatt tttcatttta
     1201 ctttaactaa taatgtttat ttggagggat tattatgtta gatgatagat tgacgtgttc
     1261 taattgtggt acattatttt actatgagga agttagaaat attgtaaaac atagaaaaaa
     1321 agaaatgcct atacgttgtc caaattgcaa tgaagttatt aagcgaaagt tgtctcatgg
     1381 ttattttgtc agctacgaac tatatgaaga aagtaaaata aatctgaaaa aaaaaccaaa
     1441 atactataaa aatttgaaaa ctggagctat tataactgaa aaagaattta aaaatttagt
     1501 gactgaagaa gaaagcaggt tttataattt gtctggtaat atttgccctg gatttatgga
     1561 tcagattatt gaaaatgagt caattgaatc aattgaaaac tatgttcctg ttgacttacc
     1621 aaaatagaaa aaataatcct gtttttacag tgaattattt taattttgat tttcaataaa
     1681 aatatattac aataataatc gtaatagcta atgaaacaat ttctcaatct ttttaccact
     1741 ccaaacaggg agcgtcaata attttgtgta aataactcgt ccttctgcaa aataatgggt
     1801 tactcagtaa acattgaagc taatgtatcc gtcacttgtt ggaagccttt atggcttcta
     1861 tttaagaatt tttgattgta tgtatcaaag atggatacta ggaaacgctc tagtgattct
     1921 tcattttgaa actgctcttt tctgcggctg tatttcttga tttgtttatt gaaagattcg
     1981 attgtagaac cagaaggaat ggagaacact tttgaacttt acaacaaaac tactgttgat
     2041 aaattaattc caattttagt tccaactgat ttaaatggtc atgatgtttt acttcgtttt
     2101 aacggatttt taggtgagga ttatagatac ctaaaaattt ctgatcctca acctttaaca
     2161 gaataataaa aaacgtgtaa tcaattattt gattacacgt tttttatttc aatcttcgtt
     2221 gtagtagtgg ttgcgacata ccgaaaggta atcttcatta cctcctacta caatctgttc
     2281 acctgaataa actggaatac catcaacaat acgagcattc attacagcct ttttattgca
     2341 aaaatggcaa atagttttaa tttcctcaat tttatcagca aatgtcaata atgcttcgga
     2401 tcccttgaag agattatttt gaaaatcatt cttcaatcca tagcaaatta ctggtacttc
     2461 ctcattcatt acgataagtg atagatcaat aatattctct ctacttaaaa attgagactc
     2521 atcgaccaaa atacaatcaa cttttccgta ctgatcaata atctccacta attctccgta
     2581 cacatccatt tcagaagtaa ttgcaatagc tggtttcgaa acacctatac gactctcaac
     2641 ttttccaaat cctgatcgag tatctattgc gcttgtcatt atgataactc tcttcccttg
     2701 ttcttcataa ttatgagcca ctttcagtag ctcaatactc tttccactat tcattgttcc
     2761 atatctgtaa aataattgtg ccattaaatt tattcccttc ttttacaatt caattattcc
     2821 ttaattccat ctgcttttat gcagctgctc gtttattcct tgatatgtct ataacatcta
     2881 ctatccctaa aaccaattta taagccatta gagatggtat tcctgaacat ctctcatatt
     2941 gttttttgag tggtatatca tctgaaaaac tgaaggaatc atgaaaatag cgacatactt
     3001 atcatttaga gttctaactt taaatgcatt tacttcttca gtcagcactt tagtcatgtt
     3061 ggacatggtt tggggcgtgt agtgatgccc atacattttc tcaatcaagt ctgcgatttc
     3121 cgacatggta ataccttttc ggaagaggtg aatgacggtc tcctctaatg tgtcattcgt
     3181 ccgtctataa gcaggaacag tctgttgctt gaactcgcca ttgcggtcgc gcggaatctg
     3241 gagatgaagt tccccatact cggtcttgac cgtacggtca taggagccgt tacgagaatt
     3301 acccgtgtta aaaccaatgc gatcgtactt ttcgtaatcg aggaaagccg ttaactccgt
     3361 tttgagaagc gtattgacag ctttttccaa atggacacgg aataatccat tcaaatcgcc
     3421 tttgttcgct agagtcttta ggatttctgt agtaaaatcg ttcatatgga agtcctcttt
     3481 tctgtgaatg tgttgtggta actctattct acagaaggga cttccttttt tctatttaca
     3541 caaaatattt tacactctca acaaaagtat cttcgattaa tacttgctca agttatgtgc
     3601 agctacgaaa aagtaggtac cgaggtactt tctttttcgc agcttttttt ggacttatga
     3661 gaagtacatt cgctggaaga atacgatata aaacgagctt tttcactaaa gatattaata
     3721 gagtgaaaga aacaagcttt tgttgtcagt tttttcactt ttctaaaaat gaaaataaaa
     3781 aattctggaa gtggagaaga attttgcttg ctatactttg tcttgtagaa aagaggtaga
     3841 caaaataatg caagctcaaa taagcgattt tccataacct tggtaggaat gacaaattaa
     3901 ataggaaagg gaagtaataa tatgaataaa aaaaagttat ctctattcgg tttttttgcc
     3961 ctgacagctt ccatggtttt gactgtttat gagtatccga cctttgcaac atcacaatta
     4021 catttggttt tctttcttct tttgggaggt attttatggt ttctaccagt tgctttatgt
     4081 gctgcagaaa tggcaacagt tgaaggatgg aaagatggtg ggattttcag ttgggttagt
     4141 caaactttag gagaacgttt tggttttgca gctatttttt tccaatggtt tcaaattaca
     4201 gtaggtttta ttacaatgat ctacttcata ctaggagcgc tgtcgtatgt aattaatttt
     4261 ccagagctta atacagatcc atttatcaag tttattggtg tattagtcat tttctgggta
     4321 ctgacttttt cacagttggg cgggaccaat cgtaccgcta aaattgctaa agcaggattt
     4381 gtcattggaa ttgttattcc ttcgatcatt ctctttggtt tagcagcagc ctactttatc
     4441 ggcggtaatc cgattcaaat ggctattaat gcgaaagctt ttattcctga cttttctcag
     4501 ctttcaactt tagtagtatt tgtatctttt atattggctt atatgggcgt agaagcttca
     4561 gcatcacata ttaatgagct ggaaaatccc caaagaaact acccattggc aatgttttta
     4621 ctagtgattt tggccatcgc cttagatgca atcggcggtt tctcagtagc agcggttatt
     4681 ccacaacatg acttgtctct cagtgctggt gtaatccaaa catttgaaac gcttatttta
     4741 cattttaaca gtcatttaca ttggttggta aaagtgattg ctctcatgat tgcctgtggc
     4801 gttatgggtg aagttagttc ttgggtagtt ggtccatcac gagggatgtt tgcagctgct
     4861 cagcgaggtc tgttaccagt tttcttccgg aaaactaata aacaagacgt tccagttccg
     4921 ttgattatga tacaaggaat tatcgttact atttgggcag cagtcttaac ttttggtgga
     4981 ggaggcaaca atttatcatt cttagtagca atctccttaa ctgttgtcat ttatctcgta
     5041 ggatatttac tatttttcct aggttacttt gtattagttt ttaagaaaaa agacttaaaa
     5101 cgaacttaca atgtaccagg aaaaactgtt ggcaaaacga ttatcggtgg aattggtttt
     5161 attttatcag tgttcgcatt gcttatctct tttgtaccac cagcttcaat tgcagctagt
     5221 gagcaccgaa cataccaaat catactactt attagctttg tagtgactgt tcttttacct
     5281 tttatcattt atgaagtaca tgataaaagt gctcacacaa ctattgagga acctactcac
     5341 gtgaaagctg aagacgttaa tcccgctgtt tatccagctg ctagaggaga gcaccacgtt
     5401 acaaaaaatg atgaacatat tttaaaacat taggaggaat tcattatgtt atacggaaaa
     5461 gataatcaag aagaaaaaaa ctatttggaa ccaatttttg gctctgcaag tgaggatgtt
     5521 gacttgccta aatataagtt aaacaaagaa tccattgaac cacgaattgc ttatcaatta
     5581 gtacaagacg agatgttgga tgaaggaaat gcgcgattaa acttagctac tttttgtcaa
     5641 acgtatatgg aacctgaagc agtgaaattg atgacccaaa cgttagaaaa aaatgcaatt
     5701 gataaatcag aatacccacg aacaacggaa attgaaaacc gctgtgtaaa tatgattgct
     5761 gatttatggc atgctccaaa taatgaaaaa ttcatgggaa cttcaacgat cggctcttca
     5821 gaagcctgca tgctgggtgg tatggccatg aaatttgctt ggcgtaaacg tgctgaaaaa
     5881 ttaggtcttg atattcaagc aaaaaaacct aacctggtga tctcttctgg ttaccaagtt
     5941 tgttgggaaa aattctgtgt atattgggat gtggaactga gagaagtccc aatggatgaa
     6001 aaacatatgt caattaatct agatactgtc atggattatg tggatgagta cacaattggt
     6061 attgtaggta ttatgggtat tacttacact ggtcgttatg atgatatcaa gggtctgaat
     6121 gatttagttg aagctcacaa taaacaaact gactataaag tatacattca tgttgacgct
     6181 gcatcgggtg gcttttatgc accatttact gaacctgatc tagtttggga ttttcaattg
     6241 aaaaatgtta tctcaattaa ttcttcaggt cacaaatatg gtttggtata tccaggtgtg
     6301 ggttgggtct tatggcgtga ccaacaatac ttaccagaag aattagtatt taaagttagt
     6361 tacttaggtg gagaaatgcc aactatggct atcaacttct ctcatagtgc agcacaacta
     6421 attgggcaat actacaactt tgtacgctat ggctttgatg gttatcgtga tattcaccaa
     6481 agaactcatg atgttgctgt ttatttagcc aaagagatcg aaaaaactgg tatttttgaa
     6541 atcattaatg atggatcaga attgccagtt gtgtgctata agctgaaaga agatcccaat
     6601 cgcgaatgga cactatatga tttatctgat cgtctgttaa tgaagggatg gcaagtccca
     6661 gcctacccat tacctaaaga cttggatcaa ttaattattc aacgcttagt tgttcgagca
     6721 gactttggaa tgaacatggc tggtgattat gtacaagata tgaaccaagc aattgaagag
     6781 ttgaataaag ctcatattgt ttatcataaa aaacaggatg tgaaaacgta cggctttact
     6841 cactaaaatt cttcagagaa atataaaaga agttaattaa ctaataaaag gagttgtgat
     6901 taccacacag ctccttaaat ctatttgaat aaatatatga tattcatttg ttacactctc
     6961 aatcttagct gttgttctat tcatcattac tcttattgta agtagttcag tcaaatttgc
     7021 ttgttcaatt gatcgataac agtatgaatt gtttgaaaag taatcggata tataattgtt
     7081 ttctcactac ctaagccttc ttggtataaa gaactatcgc aaagtataat ccgctgagta
     7141 caaaagaaag tgtcgtctac tatttctcta agttgtttac aaatgatatt actaatagaa
     7201 aagtgttctc ccaaaatgtc ttctaactgt tgtcgatagt ataaatacct aaagtctgac
     7261 ggagctacta tgaacacttc aattggagat ttccgtttat gctcatttaa aagacttaaa
     7321 ctaatggtta gtttacgaat agaattctca ttccaacgca gaaataaatt gttttgtttt
     7381 cgccattgtt caagaatttc atgaacttct aagtacaaag cgcgttgctc ttcatttaac
     7441 aagtaagtct tatcaggttg aaatatctga atatcagccc aaagtgagcg gataaacggg
     7501 agaagtgctt tatcaaaaag tatctgattt tcttcagata tattcagaga taagataagt
     7561 ttttgactga gctcaatcac caaagggtta tctgctacat ggttttgata aacaacgttc
     7621 aagtcttttt gtaacaattc aaagttatcg catagataat ttctagagtt gaatagaaag
     7681 tagatataaa ggcattcatc ttcgtcatga cataaagaga aagaaagttg ctctaatcct
     7741 ttctgtatta gctgaaacaa gggagtatgt cgggcaactc ttttttcttc ctgactaaaa
     7801 tatattgggt tagtcttaga tctctgcaaa ccgatagcga tacctaataa aataaattgt
     7861 ttttcaactg tagggtaact tcttgaaaaa aatgtttttc tacataatca atcaattgtt
     7921 gaccgctttg aataattgat ttatttagct ggatcaactc tgtccgattt gtctcataga
     7981 ttatcgttag taacaaactt cgataatcct tttcaggaat ttcaaaatgg ccatcagcat
     8041 ttaagtactg catttgttta aaaaaattaa tcaactcatt tcgtaagttg taaactttgc
     8101 ttaacgaaat gaaggtagta tcagctatat tactccaatt gtgttcccct gataaataat
     8161 gtcctagtac attgaaaaag tcagagcgct gatagatgag ttgagtgagt gccaacctcg
     8221 aaaacgaaga gtcgaatgcg actgtaaagc tacttctaga tttctccact gaaataattt
     8281 gctgatggag tatgtcggta atgcattcca agtcgttagt gacagtgagt gggcaaacgt
     8341 tcaattgatc tgctatttcg cgaatctgaa tggtcccttt ttcgtgtaat tgctcacaaa
     8401 tgtaaacttg acgaaaaata cttttctcca gataggtttc caacatagta ctcctcctag
     8461 tcattttata cataactatt ataactctac caatcggaat agtcctgtaa tatattgaat
     8521 caaaaatata aattataaaa taattagatg gggtatttgg tttaagtatc ccttatatag
     8581 taataaattt ataggtgtga ttctcgaatt aatacccgaa aatgtgagag aatttttatg
     8641 gattttttag gatacaaatt aattattttt gcttttatcc tgattaccag tccagtactg
     8701 tcggtatccc attttgcagc aaaatcatgt tgataaagtg acaagaagac tgatgagata
     8761 tattatccaa attgagaaga taattaatcg gattcttgag tataccctac tttttaagaa
     8821 agtagggtat acaagcgtat gaataaagac ataactttcc ctgtgatgga cgttcggctt
     8881 gttccaatta ccaaaataca ggcgaacgat tataatccca atcgagtagc gtctccggaa
     8941 atgaacttat tggagctttc tatcattgaa gatggcttta ctcaaccatt agtttgttat
     9001 tacaaggaaa aagaagagaa gtatatcttg gttgatgtgc ttcataggta tcgtgttgct
     9061 aaggaaagac tcaacttaaa agttgtaccg gtaacagtta ttgataaacc catagaagaa
     9121 cgtatggctt caacaattcg acacaatcgt gcaagaggga cccatgaaat aagaggaatg
     9181 gaaattgtca tccaaagatt agtaaaaaat ggatggtcag acaagagaat atccaaagaa
     9241 ttaggcatgg acattgaaga aatttttcgc tttaagcaag tgtctggtgg aatggcaaca
     9301 gtttttttga caaattttat aaggtgcaga acttctttcc gtatgctatt ccgattgtcc
     9361 ttgacaatga gcctcctcgg atgtgacgat cttcggcagg agctaacagt taaagttaga
     9421 ctgcctcgct agcgttagca catccgagct cattatcaag gattcgctgc gccaatctct
     9481 ggttacggta accaaccggt gtctcgtagc caagtgtacc gtgcaaccga aggtggttcc
     9541 accaatttac atagtcaaat aactccaaat ccaattgttg taaggtttca aatgtgtatt
     9601 gatagacaaa ttctactttc aacgacttat aagttgattc agctacggca ttatcaaaag
     9661 gacagccttt atgactcaat gatcgattga tgtcaaaagt tgttaataat tcatcaatag
     9721 cttggttatc aaactctttt ccacgatcag tatgaaaaat ctcaacctct gtcagaggtt
     9781 gtttgatacg gctaaatg
//
LOCUS       DAYYCQ010000095         9744 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000095 DAYYCQ010000000
VERSION     DAYYCQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.097"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACHAYF_002478"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACHAYF_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8579859.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="ACHAYF_002479"
     CDS             1873..4539
                     /locus_tag="ACHAYF_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8579860.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="ACHAYF_002480"
     CDS             4903..5655
                     /locus_tag="ACHAYF_002480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8579861.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="ACHAYF_002481"
     CDS             complement(5677..6831)
                     /locus_tag="ACHAYF_002481"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8579862.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="ACHAYF_002482"
     CDS             7016..9457
                     /locus_tag="ACHAYF_002482"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8579863.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9614..>9744)
                     /locus_tag="ACHAYF_002483"
     CDS             complement(9614..>9744)
                     /locus_tag="ACHAYF_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8579864.1"
                     /translation="SNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         3134 a         1756 c         2079 g         2775 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ctgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttggc tcttcgtcaa
     9541 attgtgttgg cgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc
     9601 gccgcatatt tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa
     9661 agttataaaa gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca
     9721 gcccctcagt gacgccgttt gagt
//
LOCUS       DAYYCQ010000096         9643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000096 DAYYCQ010000000
VERSION     DAYYCQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.098"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1108
                     /locus_tag="ACHAYF_002484"
     CDS             134..1108
                     /locus_tag="ACHAYF_002484"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8579865.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="ACHAYF_002485"
     CDS             1090..1551
                     /locus_tag="ACHAYF_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8579866.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="ACHAYF_002486"
     CDS             1601..2065
                     /locus_tag="ACHAYF_002486"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8579867.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="ACHAYF_002487"
     CDS             2079..2828
                     /locus_tag="ACHAYF_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8579868.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="ACHAYF_002488"
     CDS             2828..3658
                     /locus_tag="ACHAYF_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8579869.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..4305
                     /locus_tag="ACHAYF_002489"
     CDS             3658..4305
                     /locus_tag="ACHAYF_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8579870.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4403..5179)
                     /locus_tag="ACHAYF_002490"
     CDS             complement(4403..5179)
                     /locus_tag="ACHAYF_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8579871.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5323..6282
                     /locus_tag="ACHAYF_002491"
     CDS             5323..6282
                     /locus_tag="ACHAYF_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8579872.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6399..6707
                     /locus_tag="ACHAYF_002492"
     CDS             6399..6707
                     /locus_tag="ACHAYF_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8579873.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6734..7033
                     /locus_tag="ACHAYF_002493"
     CDS             6734..7033
                     /locus_tag="ACHAYF_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8579874.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7207..7758
                     /locus_tag="ACHAYF_002494"
     CDS             7207..7758
                     /locus_tag="ACHAYF_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8579875.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7904..8197
                     /locus_tag="ACHAYF_002495"
     CDS             7904..8197
                     /locus_tag="ACHAYF_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8579876.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8190..8486
                     /locus_tag="ACHAYF_002496"
     CDS             8190..8486
                     /locus_tag="ACHAYF_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579877.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8557..9561
                     /locus_tag="ACHAYF_002497"
     CDS             8557..9561
                     /locus_tag="ACHAYF_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8579878.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         3418 a         1374 c         1798 g         3053 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa atttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtattgtaaa gttatatgac tttataatac ttattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtatcaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgcatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttaataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgacttc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     4321 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     4381 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     4441 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     4501 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     4561 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     4621 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     4681 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     4741 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     4801 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     4861 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     4921 atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc
     4981 cccttaatcg ttcaactgtt ccactttctg ctaaaagttt aaaagctttt cgaatggtaa
     5041 tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa
     5101 gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaaa tataatggtc
     5161 tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt
     5221 tattaaaacg tcgattgtaa aattaagcta gacaactaaa aagatcattt gtgctacact
     5281 caaaatagtt ccgaccaaag aattaataag gagtgagtac aaatgactta cacccatctt
     5341 acaacagacg aactagtaat catagagtct tatttcaata tcgagaaacc tgtcgctctt
     5401 gtcgcacaaa gtttaaatcg ctctagacaa accatttata atgtttattc gtttctcaaa
     5461 caaggaaaaa aagtgctaga ttattaccgt caatacaaac aaaataaatc tcgttgtgga
     5521 cgacgttcaa ccgttttatc tgatgaacaa tcaacatatg ttcaagaaaa ggtagctcaa
     5581 ggatggtcac ccgatgtgat tatcggccgt ggcgagtttc caattgattg ttctgttcgt
     5641 acactctatc ggatgtttaa aaaaggcctt tttgacacac agtcgttacc tatgaagggg
     5701 aaacgtaaac cgaataatca tcaagaaaag cgtggaaaac aggcctttcg cagaacgatt
     5761 catgaacgga acagtctcta tccacacctg actaaagaat ttggtcatct cgaagtcgac
     5821 accattgtgg gagttaaaca caagagcgct gttattacat tggttgagtg tttatccaag
     5881 gttattatta ctttgaaacc gacaggcaga cgagccgaag atatcgaaaa caggttaaat
     5941 gattggttca aatccatgcc taaacacttg tttaaatcca tgacatttga ttgtggaaaa
     6001 gaattttcca actggcgatc tataagtaac tcgaacgata ttgccatctt tttcgcagat
     6061 ccaggaacac cttcgcaaag agggctaaat gagcactcaa acggcttgct tagacgagat
     6121 ggcttaccta aaacaatgga ctttagggac acggatgaaa cctttattca atccgttgcc
     6181 tcgaaaagaa accatattcc aagaaaatca ttaaattatc gaacaccatt ggaagtattt
     6241 ttgagctgca tagataaaga catattgtct agcttaattt gacaaatcag atagtataaa
     6301 atgaatcaaa ttgaatatca aatatatatt atatccatga agcccaatct tctttaatag
     6361 aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat aaaggacaac
     6421 gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct ttatgttgga
     6481 atctaatcga tcaaaacgtt caaaagcaat ttcatttaga ctatcttcaa atctttgaat
     6541 tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaacca gaagcaagaa
     6601 aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa atatgggtca
     6661 ttgattcggg tgagtatcaa acgatgttat taccagaaga atactgatat ctaaaagaaa
     6721 ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg cacagctagt
     6781 cactactcct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg aaattgaaat
     6841 gggtatgctt cctgaagatg ttactctacc acgtttagtg atgacacatc tttatattta
     6901 ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata ttaccagcca
     6961 gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc agattggagg
     7021 aacgaatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa gatatcctta
     7081 cagcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt ttcaactagt
     7141 attgacactt ataacggcaa tggtgattga attgcttgaa caaaaattag cataggagaa
     7201 aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg agatcctcat
     7261 catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga agcggaagat
     7321 aaattagcgg tagtcattac tattcgtcca acagatacag aaccatttac gaatgatttg
     7381 agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt tattgctgtg
     7441 aatctttttt cgtatacaaa agcgaaaagt cctcgggtat ttcctcgtgg aaatgatgaa
     7501 caatctttgg aaatattaac aactgttttc acagaaaaga aaatcaacac cattattttt
     7561 gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcgaa gaccatttac
     7621 gatcaattga gtgcgaaaca aaaaaagatg accaaagtat tgataaatgc agaaggtaag
     7681 ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga tagtcaatta
     7741 ttatttcaag aaaaataaca ttttattttc ttatgttact tataagtagt tttacatgct
     7801 ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat gtacccagta
     7861 atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga ttgatgaaac
     7921 ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg aactggattc
     7981 agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt ttccaatgaa
     8041 aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac aggaaaaaac
     8101 tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga ttcaaaaagt
     8161 aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta ggtggtttaa
     8221 tttacggtgg atattggtta tataaatata tgcttgcgca tccgaatata gataaaggaa
     8281 aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact aagaaaaata
     8341 atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga agggaaattt
     8401 ctactagttt taatccatct gaaagccctc aaggaagaga tcgttgtcat gctggctatc
     8461 gacattcttg ggaaaggatt aactaaatga acaccattga gattaattga ctgataaaat
     8521 aagtacgcga gggagaaatt gaaaggggga aataaaatga aaaaatggtt gttattacta
     8581 attggaatgg ggattttatt cactggttgt acatctgaga atacgaggac taaagagact
     8641 gtagaaactg atacaacaat tgaggctaaa aaaagtaaaa cgcaatcaac tgaacaaact
     8701 acaacagaaa tgaaacaatc tgtggaagca agtacaagtg aaagttcaat cattgaatct
     8761 tcttctgaag aggttaaaaa tactttgtgg gatagtgaga aatctactca actagcaatt
     8821 tttgtcccac aatggggaaa gacattaggt caagaatata aaagctacaa tcctcagaac
     8881 aatgtaagtc tgtatggtac gcctttacca agtgcagtta ttgatggaaa ttggaaaatg
     8941 gcaatcaatg aagcaccagt taccgtacaa tggtcagaag atggaactgg tcatgcagat
     9001 ttcaatttag tagcagttta ttctgatgta gaaactggag aatatttagg gcagcatgta
     9061 tatttctttg gctttcaaaa tggacaacct aaagtttatt taacgcaaca aaatcaaggc
     9121 aatgataaaa actacatgta cttcaatgaa actcagaacc aacagttaaa acaaggtttc
     9181 attgatattg tgaatggaca aactcctcaa actccagtag tagaagaatc tactcaacaa
     9241 gcaacaacta caacgacaca atctacaagt agctcagcaa tttcaagttg ggaagctgct
     9301 aaagaaactg tactaaataa taaagcttta tggttgtatg attctcctga taaaaatgca
     9361 tcaaatacta atttaattag cacaaatgaa tatatgaaag atctaatgca agatgaaaat
     9421 ggaagctact attcaattaa tattgtaacg gatgataatg gaaatacaac aatggctggt
     9481 ggaaaacaat tccgtgttta tacagatgga agaatttctc aacgtattgg aatgactaac
     9541 aattttcaaa tgattaaata attgaaaata ttatttatca aagaaaaact gattttgaga
     9601 aaaattattt aattttctta gaatcagttt ttttataaag taa
//
LOCUS       DAYYCQ010000097         9312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000097 DAYYCQ010000000
VERSION     DAYYCQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.099"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..711)
                     /locus_tag="ACHAYF_002498"
     CDS             complement(43..711)
                     /locus_tag="ACHAYF_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8579879.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            845..1132
                     /locus_tag="ACHAYF_002499"
     CDS             845..1132
                     /locus_tag="ACHAYF_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579880.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1548..3086
                     /locus_tag="ACHAYF_002500"
     CDS             1548..3086
                     /locus_tag="ACHAYF_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8579881.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3083..4048
                     /locus_tag="ACHAYF_002501"
     CDS             3083..4048
                     /locus_tag="ACHAYF_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579882.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4035..5357
                     /locus_tag="ACHAYF_002502"
     CDS             4035..5357
                     /locus_tag="ACHAYF_002502"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8579883.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5420..5524
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5598..6341
                     /locus_tag="ACHAYF_002503"
     CDS             5598..6341
                     /locus_tag="ACHAYF_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8579884.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6356..7348
                     /locus_tag="ACHAYF_002504"
     CDS             6356..7348
                     /locus_tag="ACHAYF_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8579885.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7360..8097
                     /locus_tag="ACHAYF_002505"
     CDS             7360..8097
                     /locus_tag="ACHAYF_002505"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579886.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8087..8809
                     /locus_tag="ACHAYF_002506"
     CDS             8087..8809
                     /locus_tag="ACHAYF_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8579887.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8893..>9312)
                     /locus_tag="ACHAYF_002507"
     CDS             complement(8893..>9312)
                     /locus_tag="ACHAYF_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8579888.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3051 a         1604 c         1967 g         2690 t
ORIGIN      
        1 atgatatttc tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc
       61 aagaggatca agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac
      121 agatccaatt tccagatact tcactttatt caatgtataa gatagaatag aacagctaag
      181 taatgtatac agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa
      241 agtaatcaag aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata
      301 aatgatatct gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc
      361 agaaagaata cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg
      421 aaggctctcc cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag
      481 aaaagcgtgt tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag
      541 tgaagtgaca gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac
      601 cccaccatct gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc
      661 tgcgcctaaa atgattccgc acaattctgt aaataaattt aattttttca tttctagcct
      721 cttttctttt gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa
      781 gaggaaaatg aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg
      841 aaaaatgcga gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta
      901 tcaaggagtt tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa
      961 taaagcaaat caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga
     1021 catctttctt tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga
     1081 actcattcct ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat
     1141 aagaaaacaa gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt
     1201 gtcatgatac agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat
     1261 ttctacttta ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat
     1321 agctgattcc aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag
     1381 aaccaaaaag ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg
     1441 tatttttttc ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa
     1501 aaggaaagaa atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta
     1561 tacaaacatt aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca
     1621 tttctctcat ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag
     1681 ccaatcataa aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc
     1741 cttcacttgc cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat
     1801 tgattgcact tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag
     1861 ctgaaattga cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac
     1921 gcttgctgaa agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg
     1981 ccttagtttt aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag
     2041 ggacatttat tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga
     2101 tcagttctgc cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga
     2161 aacctcgata ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt
     2221 tatatcagtc tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg
     2281 aacctgaaat cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag
     2341 ttgaattaaa accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc
     2401 aaatcgatat ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg
     2461 aagcaatgaa aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat
     2521 tgaataagca attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct
     2581 tggcagtcaa gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag
     2641 ctatctctga tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat
     2701 ataaaggaat ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa
     2761 gtttgcgtta ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag
     2821 atagccagct caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag
     2881 cttatcaagg cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta
     2941 aagcactgaa taaattaagc ggaaaaataa cagaggatca aatgattgat atgaattacc
     3001 aagtcaatgt cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag
     3061 gattagtaaa ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa
     3121 gtatgatgga aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt
     3181 tgttttggtt ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt
     3241 atctaaacca tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct
     3301 gcagaaaatg cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat
     3361 gacagtcaag caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag
     3421 agcagaccgt gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga
     3481 tcgtatgcct agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat
     3541 tgctgcaagc ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc
     3601 aagaaactca ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt
     3661 tttcgttacg cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa
     3721 ggatggagaa attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga
     3781 ctacgttcgg catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt
     3841 agtcgtcaat ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt
     3901 tgatacgcct atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt
     3961 agaaaagcaa agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg
     4021 agaggaggaa ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct
     4081 taagtgctgc ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc
     4141 tatggggcgg gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag
     4201 tagaggcacg taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg
     4261 ccaactggga agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc
     4321 gaaaaaaaca attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg
     4381 atgattcatc tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa
     4441 ccgggcaaag acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag
     4501 actttttatc attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg
     4561 cttttgagct agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata
     4621 atcaacggcc attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg
     4681 aagcatctgg cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta
     4741 ctcgctatcg tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga
     4801 ctgggagaca gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata
     4861 aacacggaat tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg
     4921 gagatattgt ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt
     4981 atgtagctaa gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta
     5041 cgatcgtcta cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt
     5101 cgtcagatca agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg
     5161 aaccagttgc taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag
     5221 tgattagcga gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat
     5281 tgactaaaaa agagttggat agctatatca tggggtatcc gacattggca agtgatctct
     5341 cctatttatt gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg
     5401 ctacacttta aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt
     5461 tgtgaactta gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga
     5521 cgcttgaagc gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga
     5581 agaaaggagg taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt
     5641 taattatttg gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac
     5701 ctgctgctgt attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca
     5761 cgacattaaa tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg
     5821 ccttggcaat ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt
     5881 cacaaatgtt gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta
     5941 tgcttccaaa agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt
     6001 cggatacatt gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag
     6061 atacagggaa gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg
     6121 gtttaaaagt ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg
     6181 ctcaagcagg cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag
     6241 tttttgctgc gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat
     6301 tattgaaaaa agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa
     6361 aaaattatgg cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga
     6421 aacaagtagc aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt
     6481 accgaatact aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc
     6541 tggtttaaat gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg
     6601 tgcggacaaa gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga
     6661 tggacagaat ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt
     6721 cgccacccta tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc
     6781 aggttggcaa agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg
     6841 ggacttttca aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa
     6901 atcctcagat atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg
     6961 tattgaacta aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc
     7021 ggtaattatt acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat
     7081 ggatgccacg aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact
     7141 gttgcaaaaa tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt
     7201 gtcaaaaaat tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg
     7261 caattataca gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga
     7321 actattcact aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac
     7381 ttgaatgtag tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa
     7441 gataccttta catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact
     7501 ttaacaggag tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact
     7561 ggtctccata cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc
     7621 gtctatcaaa acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa
     7681 gtaagggatt atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa
     7741 ttatctggcg ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct
     7801 tacttgcttc ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa
     7861 gactggatgt tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat
     7921 atcgaagaag caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat
     7981 gtgattgctg agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa
     8041 tcagagttga agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc
     8101 tcattgtcat ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt
     8161 gacgatcatc aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag
     8221 taagactcaa gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga
     8281 acaagttgag ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat
     8341 ttggacgaat gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc
     8401 agccataaat gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa
     8461 ttatatccaa gcttacccta atcactattt gatccattgg tattcttctt tggactatca
     8521 acaagactat attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc
     8581 agcagtctgg caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg
     8641 actacatgca gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact
     8701 gcttgaagaa cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa
     8761 aaaaaatact tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt
     8821 gacacaacta gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt
     8881 aataaatgaa tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga
     8941 accataacgt gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc
     9001 accatttaag taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt
     9061 gtaagaaccg cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg
     9121 agtagcagca gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg
     9181 tttttccgtt tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg
     9241 aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg
     9301 ttgtcctgag ta
//
LOCUS       DAYYCQ010000098         9274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000098 DAYYCQ010000000
VERSION     DAYYCQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.100"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACHAYF_002508"
     CDS             162..392
                     /locus_tag="ACHAYF_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8579889.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACHAYF_002509"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACHAYF_002509"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8579890.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACHAYF_002510"
     CDS             1803..1979
                     /locus_tag="ACHAYF_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8579891.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACHAYF_002511"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACHAYF_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8579892.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2474..2941
                     /locus_tag="ACHAYF_002512"
     CDS             2474..2941
                     /locus_tag="ACHAYF_002512"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8579893.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="ACHAYF_002513"
     CDS             3026..3910
                     /locus_tag="ACHAYF_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8579894.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="ACHAYF_002514"
     CDS             4024..4260
                     /locus_tag="ACHAYF_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8579895.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="ACHAYF_002515"
     tRNA            4348..4428
                     /locus_tag="ACHAYF_002515"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="ACHAYF_002516"
     tRNA            4435..4506
                     /locus_tag="ACHAYF_002516"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4632..4802
                     /locus_tag="ACHAYF_002517"
     CDS             4632..4802
                     /locus_tag="ACHAYF_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579896.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5022..5729
                     /locus_tag="ACHAYF_002518"
     CDS             5022..5729
                     /locus_tag="ACHAYF_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8579897.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="ACHAYF_002519"
     CDS             complement(5788..6432)
                     /locus_tag="ACHAYF_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8579898.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            6559..6846
                     /locus_tag="ACHAYF_002520"
     CDS             6559..6846
                     /locus_tag="ACHAYF_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579899.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="ACHAYF_002521"
     CDS             6954..7274
                     /locus_tag="ACHAYF_002521"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8579900.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            7355..8668
                     /locus_tag="ACHAYF_002522"
                     /pseudo
     CDS             7355..8668
                     /locus_tag="ACHAYF_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            8728..9084
                     /locus_tag="ACHAYF_002523"
     CDS             8728..9084
                     /locus_tag="ACHAYF_002523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579901.1"
                     /translation="MSKSRLPQTCSVCRAQFHLLEAKGCAGCQKPIHLKYEETKKKAN
                     TSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWINRYKFMGDIRLAGTFAYKWTQ
                     IKKRLPGIYLLSNTFI"
BASE COUNT         3061 a         1531 c         1990 g         2692 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaggttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgacg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgtg gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gtcgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgataa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cgctaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc gattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgggtagcac
     2401 tgagcgataa agaaaaagag gaaatttatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag taaccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca gctagcttca aaccaaactc ggatcattat
     2761 acgtactggc actactagta gcgctgcgtt acacctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag tgattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aaacgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aataaataaa ggggcacatc tttacggtgg
     3481 agaatttggg ttgatgttct tagatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggggttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 gtacagaaac gatgctaatc ttataggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 agagaattaa gagctttaga gaaggtgcag cttgcataaa agaagagaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatatt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt tttgctttgc
     4561 taccttgttc cttgaagatt ctgctgaatt aagatagagt taagttaact acaagaattg
     4621 gagggatcca catgaaatgg aaagaaaaag tagacgaagc agctgaaaag ttatatgatt
     4681 tggtcaaatc agaaaaatac aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg
     4741 tccaggtaaa atcaaaacgc ccaacgaatc atacaaaaaa atggatggct aaaaaccgct
     4801 aataataaca aaagatccat aagtcggcat tttcatcgat tttagggtct tttgttattt
     4861 tctaggttga acatatctaa aaaattgttt ctgacaatag gagtagggag aacgatgtgt
     4921 gtctaaatcc tctgttgatg gatagaaacc tttatctttt tcagttgtga atcaggtata
     4981 taccagttat aatagaagaa acgaaaggac gggtgtacgt tatgtcgaat caattacctg
     5041 tatatataca gattcatgac caattaaaaa atgaaatcga acaaggtata tggaaagtcg
     5101 gagaccgtct tccttcagaa agagaattag cagtgaaatt caatgtcagc cgtatgacgt
     5161 tgagacaagc aattcaaaac ttggcagatg aaggaatctt agaaagaaag atcggttcgg
     5221 gtacgtatgt tgcgcgagaa aaagtacaag aaaaaatgtc tggtgcaaca agttttacag
     5281 aaattatgga atcacaaaat cgagtaccgt ctagtcggac aatctcttat ttcttgactt
     5341 cgccaagctc aagtgaaatg gaaaaactaa acttgagtaa agatgacacg atcttgcgaa
     5401 tggaacgtat tcgtttcgca gatgatgtcc ctatttgttt tgaagttgcc agtgttccac
     5461 aaaagctgat cgatggctac agcaaagcag agattactga atcgttgtat cgcacgctag
     5521 aagaaaaagg cgggaaaaag atcggtccag cgaatcaaac gattactgct gtgttggctt
     5581 ctgagaaaat tgctgactat ttagaggtaa aacgaagaga tgcagttctt cgtttgcgcc
     5641 agatttctta ttttgaagac ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa
     5701 atcgttttga gttttatttg gaaaaataaa aaacaggtga tccctgattc cttattagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttatcagtt gctcgtgata
     5821 agaaacagaa ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtta gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagaccgcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgcccg ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaat tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aattgctgta gtatgcgata ataaagacaa
     6541 aggcaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataataaagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa aatatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actctgatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagacg tggatgtgtt gttattaggc ccacaagttc
     7141 gctttatgaa agctgatttt gagaaacgtc tggaaccaaa aggcattcct ttagatgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcgcgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gttggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggtgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgctggg ctagctcacg ctgtgcaaac
     8041 agcaatcaga tcaaacggcc gattcatcta cttaagtgcc acgcctgatg agaagttact
     8101 aaaggaaatc aagcagacat ttgagctcca caaacttgcc ttgcgctttc atcggcgctt
     8161 gcttcctgaa cccgtgcttg ttttcatgaa taattggcga caaaaatgtg gaaacaaaaa
     8221 gaaaatcaac tctttggtgc ggatcattca gcaactaatc aaagaaaacc gtatcctttt
     8281 attttgtcca agtattacga tgatgcatcg tttaaaaatc acactgcaag aagcttttcc
     8341 agaatatgag ataacagatg tttcttcaca agacgatcaa cgggcaaaga aagttcagaa
     8401 aatgcgagaa gcaatatacg agatactgtt aaccacaatg atcctagaaa gaggagtcac
     8461 atttgaaaga atctctgtca tcgttttagg agcagatcat ccagttttta ctaaatcctc
     8521 tttggtgcaa atcgcaggaa gagctgatcg taaaggtcct tatacgaaca gtcgagtcta
     8581 ttttttctat gaagaaaaaa cttcagctat tcgaaaagcc tgtcaggaaa tcaaagaaat
     8641 gaacgaagaa gggaagcaga tgctgtgaga tgtagttgct gcggtaaaga catcattcaa
     8701 aatttaacat tcagcgagat catcggagtg tcgaaatcta gacttcctca gacatgttca
     8761 gtttgccgcg cacaattcca tttattagaa gcaaaaggtt gtgcaggttg tcaaaaacca
     8821 atccatttga aatacgagga gacgaaaaag aaagcaaata ctagtttcgt ttactgcaac
     8881 gactgtcaag aatggaagaa gaaatatcct gcatatgatt ttcaacaccg cgcctttttt
     8941 acgtacgatg aaaagatgca ggcttggatc aatcggtaca agtttatggg agatatccga
     9001 ttagcaggga cttttgcata taaatggact cagataaaaa aaagattacc cggcatatat
     9061 ttattgtcca atacctttat ctgaaaaaag gtggctagaa agaggattca atcaagtaag
     9121 cgagatgttg aaagcagcaa aaattccttt tcagatgctg ctcaaaagag agcaacatct
     9181 gcttgctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcagc cttttgagct
     9241 tgccgtacct ctttctaaga tacaacataa aaaa
//
LOCUS       DAYYCQ010000099         9189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000099 DAYYCQ010000000
VERSION     DAYYCQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.101"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..200)
                     /locus_tag="ACHAYF_002524"
     CDS             complement(<1..200)
                     /locus_tag="ACHAYF_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8579902.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQ"
     gene            401..1615
                     /locus_tag="ACHAYF_002525"
                     /pseudo
     CDS             401..1615
                     /locus_tag="ACHAYF_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1794..2129)
                     /locus_tag="ACHAYF_002526"
     CDS             complement(1794..2129)
                     /locus_tag="ACHAYF_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8579903.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2113..2376)
                     /locus_tag="ACHAYF_002527"
     CDS             complement(2113..2376)
                     /locus_tag="ACHAYF_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8579904.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2836..3639)
                     /locus_tag="ACHAYF_002528"
     CDS             complement(2836..3639)
                     /locus_tag="ACHAYF_002528"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8579905.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3617..4741)
                     /locus_tag="ACHAYF_002529"
     CDS             complement(3617..4741)
                     /locus_tag="ACHAYF_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8579906.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4757..5572)
                     /locus_tag="ACHAYF_002530"
     CDS             complement(4757..5572)
                     /locus_tag="ACHAYF_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8579907.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5562..6434)
                     /locus_tag="ACHAYF_002531"
     CDS             complement(5562..6434)
                     /locus_tag="ACHAYF_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8579908.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6519..7808)
                     /locus_tag="ACHAYF_002532"
     CDS             complement(6519..7808)
                     /locus_tag="ACHAYF_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8579909.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7952..8464)
                     /locus_tag="ACHAYF_002533"
     CDS             complement(7952..8464)
                     /locus_tag="ACHAYF_002533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579910.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8531..8680)
                     /locus_tag="ACHAYF_002534"
     CDS             complement(8531..8680)
                     /locus_tag="ACHAYF_002534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8579911.1"
                     /translation="MTMITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLI
                     LAHDR"
BASE COUNT         2849 a         1942 c         1472 g         2926 t
ORIGIN      
        1 atctgagtgc gtgtcgtata ggaaccgttg cggataatac gaaccgatcg acagtgttca
       61 caataggaag gagagtcatc caattttcct ttaataaact gcgcttgaac gcctttatat
      121 tctttttcaa tcagccattc ttcatcaaaa aataaatgtt tatctgttaa tccgagtaat
      181 tttctagtat gattatccat tgagagactc ctttggtagg atgacgtttt ggtcgactaa
      241 attttaccaa aagtgagtct ctttttgagt tgaaacgaag aaaaagtgtt agtagaaaat
      301 cgccttcgat tttccaccaa cacaaaatat tatagaagca aaatcaattc taccttagat
      361 gttcttatat cactctatca gtcctatttg acaaagagcc aagaaaaaga cgttggtcgt
      421 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      481 tggaaacggg aaagcaatta tagtgaaaaa tgggaagaag gaaacgattg tccgttttga
      541 acaatacaat catatgcctt tggttacgcg cctaaaaaag caacgctata cctgtaaaaa
      601 ctgtcgtacc cattggacgg ctcaaagtta ttttgtccga ctcagccatt caatcgcaga
      661 tcatgttcga tataaaattg cttctttact gactgaaaaa gtatctttgt cttttattgc
      721 gaaaagttgt caggtatctt tgactaccgt tactcgtaca ttgaaagagt ttaaaagcta
      781 tttaccaaag ccttctaaga agattctccc cagagtattg atggttgatg aatttcgttc
      841 gcacgcttcc atagaagata aaatgagctt tatttgcgca gatggtgaaa caggaaactt
      901 aattgctgtc ttgcctacgc gtaaattacc tcgattaaca agctatttct taggctgcac
      961 caatccagaa gaagtagaat tcttagtcac agacatgaac gccgcttact tccagcttac
     1021 caaacgtgtt ctgccaaatg cgaaagtagt gattgatcgg tttcatattg tcaaacacat
     1081 gaatcaggca ctcaatgagt tgcgtatccg tgaaatgaat gagcttcgta aagcaggaca
     1141 gaaaagtcag gcagaaaaac tgaaaaagaa ctggcgcttt ctgctaaaaa atcgtgcaag
     1201 catcaaccat tatgagtaca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1261 cactgaagca atgatgattg atcgattgct tgagttttct gcgcctctaa aggaggcgta
     1321 tccctttttt catgaattag tcgaagcctt tcgagacaaa gaccctgact tatttttctc
     1381 cttattggca gaacttcccg aaacgttaga tgacggcttt cgggagaagc ttcaaaacct
     1441 tctgacctat gaagaaggta tcaccagcgc aatgatctat ccttattcca atggaaaaat
     1501 tgaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1561 cgagaacatg agaattagaa tctttttgat caatcaatta atcaatgtaa gataacaaaa
     1621 aatctgaacc agaatgtggt tcattctgac ccggatttga ttttgcacaa ctcatcagtc
     1681 cttattgaca aagagccaaa aatctgagcc agaatgaggt tcattctgac ccagatttga
     1741 ttttgtacaa cccatcagtc cttattgaca aagagccctt ctttttagta ttattataat
     1801 tcttccatgc gttcttccag tcgctgtaaa tcgcttggac ttaattcccc aatttttttc
     1861 catttgacct ttttgttgtt tttgggtaat tcgtttagag ttcctacgtc aatccaagat
     1921 tcttttttta gtcctgcctg tttccaatcc cttatttcaa cgtatttcag ctttatttta
     1981 tcggatttag tatgatattt tgaagtaatt gacagaaacg aaattgtgtc cagtccattt
     2041 tttagaatat agacaggccg gcgtttaccg ccctctgcag ctgtgaaagc aacgtagacg
     2101 gttgctatat cattagtatt catcttcatt gaaccattct tcaatttttt cgtcagtgtc
     2161 tagtttttgg acaggcaatt tttccgctaa tctttgaata ttatggattc gtttactttt
     2221 ttccgacaat tcaatagaaa atgggatttt tccttgctca acaatttgct tataaaaaac
     2281 ggtgacggct gttttggggc tcatgcctag ttcatcaata atgctttctg cttcattttt
     2341 caatttttcg tccatagaaa tttgtaactt agccattttc tccacctctt tctgacaaac
     2401 acattatatc acggaaagag ttcaaatgga attaatacaa ctacttttga actccttgaa
     2461 atgcagtgta tttatctaaa tataaggaaa ggatatagtg ctaaatggcc ttgatagata
     2521 gaaaagtttc aaaatccgcc acagcgataa ttgaagcgaa cactcaacga cacacagaac
     2581 aggaaaagac tcctaaaaga accaaattaa ctaggacgtg tctgaaaact ccacaaagat
     2641 gtggtatgct ctggtaaaaa acgaatggca caaccacgaa gaccgatatg gttcttggaa
     2701 aactttctcc accctttttg taattggagg gatagtggtt tattgatagc gattttccaa
     2761 gttcttcaag tggaaccaga cattgagcac cacggctaaa aagtgaatga agtctttatt
     2821 tttaatcaat aatttttaat gattaccagt aattggtttt ttcatgcgcc ctatttctat
     2881 ttgatgtgga gttaaataat gctgataagt aaaaattttt ccgtcaatta aactgccact
     2941 agtgaatcct gttctattgg taaaaagaga atagtctggg tattcatcta caccaaatga
     3001 taaagattca gccataaact gttttttacc agcaaattca gcactgctac cttgatggat
     3061 atgtcctaca aaaataccta aaatatcact ttcggcaatc aattcagcaa aaccttccgg
     3121 aacttcagtt acaatagatg gatcttccca cgacaatgga tgatgaacca ataaaatcgt
     3181 tcccttttca gcaggtgtac ccaaccactc ttgtaattga atcatctgtc ctatcgatat
     3241 ttttccttcg tgctgggtat cttgagcaga atccaaggaa ataattcgta acccattttg
     3301 attcgtacaa tggatatact ctcccgtttc attgctttca acactcattc cttcaaagaa
     3361 aacactgcga cgatcatgat tcccacgaca aaaatagtaa ggtgtggttg ggaaatattg
     3421 atccaacatt ttccgtaaga cttgataatc agaaatcccc ccttcatgta ctaaatcacc
     3481 tgttaataga ataaaatcgt acactgttaa cgagagttct tgtaacaatt tttccaatgc
     3541 caactgtgga tttttaattg ttggaaatag ttcagcagta aacaagttag tttgatagtt
     3601 ttctctaaaa tggatatcag agagatgcaa aaagcgcatg gctattccct ctttcttcaa
     3661 taaaataatc aggatatcct aaattttctt gcgtctgata atcaaaaaaa tgcattcttt
     3721 caggttctat gcggagattg actgcatcac caatctgcca agtaggtttt tcattaagga
     3781 caatcatttc tataccatcg actttaacgg tgtacgcata actttgacct aatagctcac
     3841 gatacttcac ttgaccgtgt aaatcgcttg tttcttttga aaaggttacg tgttctggtc
     3901 ttatgcctaa atacaatgag ccactaacct gaagaagttg tttttgccaa gctggattca
     3961 atgttaaaaa ttgactggct acctttaatg ttccttgatc ataagtaact ggcataatat
     4021 tcatgcctgg cgaaccaata aaatttgcag taaaagtatt tttaggtttc gtataaacat
     4081 ttttcggcgt atcaatcatc tgaattctac cttcattcaa taaaacaatc cggtcagcta
     4141 acgtcatcgc ttcaatttga tcatgagtga cgtagacaaa agtttgttta taacgttgat
     4201 gaatttttac caattctttt cgtgcatcca atcgtaaacg cacatccaaa tttgataacg
     4261 gttcatctaa taaaaaataa ttactccgtt taaccaccgc acgagccaat gctacacgct
     4321 gccgttgccc tcctgacaaa tctttaggaa gtcgatcttg gtatggcgtt aaacctaaca
     4381 tcttcaaagc ttcttccaaa cggtgctgaa tgtcagctgt cttaatttta ttcgccctta
     4441 atgcaaaaat aatattctcc tttacagtca tgtgaggata taaagcataa ttttgaaaaa
     4501 ccattgcgat ctctcgttct ccacaaggta gcttatttgc tagttgtgta cctaaaaaaa
     4561 gctctccact cgtaattttt tccaaaccag caatcattct cagcaaagta gattttcctg
     4621 aaccagatgg tcccagtaac acaattcgtt ctccagcggc cacagctaaa tcaatttctt
     4681 ttaaaatggt atgttctcca tatttttttc cgatatttct taattcaagc gtttctccca
     4741 tgatttgtcc tccaatttat ttaatacctg cagaaacaaa agtcccgata atatattttt
     4801 gaaaaattgc atacaagact aacggtacaa acattgtaat taaactaatc gccattgcta
     4861 cggtaaaatt tgtcccacct tcagaactaa tataggtctg catcgccaac ggcaaagtat
     4921 aattatctac tgtccgtaaa attaataccg gccaaacata ctcattccaa gaggtaataa
     4981 aaaaccaaat accggtagaa gtaatcgcat gtctagtaag aggaaaaaca attttactca
     5041 tgacggttgt ttgtttgatg ttatccaatg ccgccacatc tagaaggctc actggaattt
     5101 cccgcattgt ttgcgtcaat aaaaaaatgc ccatcgcatc agcaaattgt ggtattatca
     5161 caccccaagt agtatcaatt aaattaagtt ttgcaatcaa caaataatta ggaatcatcg
     5221 caacagtaaa aggaatgaac atcgtaccaa tgatgataaa gtaccagatt ttcttccctt
     5281 tgattcggca ataagtaaaa ctatatgccg caaaaaaagc tacgcctaac ttaacaaccg
     5341 ttataattgt agcaataaaa aaagtatttc tcgtaatttt gataattgga agattttcga
     5401 acaaatagca aaaattttcc aaggtaaacg gctgtggtat caaactagta atcgtctgat
     5461 aggcatcttt caatggctta aaagaattgg ccataataaa caataatgga tagataataa
     5521 tgattatgat gaacataaat aagagatgtg gcaaccagcg tttaattttc atagtaaacc
     5581 cccttttcca caaatttaaa tattacccct aagaaaacag caaatattaa taaagttaaa
     5641 actgaaattg ctgaagattc tccgacttta tacatttcaa aagccttttg ataagcataa
     5701 tagatcacgt ttgcactacc attattcggt ccaccacgag tgatgatctt aatcggagta
     5761 aacacatact gcagaccttg aacaatggtc atcaccaata aatatagtcc tgtcgaacta
     5821 ctcataggca acacaattgt aaaaaaaatg cgccagatgg gtacacgatc caacttagct
     5881 gcttcgatca tctcttgatc aattcccaca attgctgcaa aaatgacaat gaaattataa
     5941 ccaaaaactt tccaagcagt aatacctgca atcacagcaa ttacccaacc ttgtaaattc
     6001 atccacatag gcagtcttaa accaaaattt cccaacaaga cagcaattgg tcccgatacc
     6061 ggatttaata tccatgtaaa aaccatcccc gccactacca aagaaatcac agctggaata
     6121 aaaaataatg gtttatacaa attctgccct ttttctataa ctattcctaa taaaaaggat
     6181 gtaatatatg ggaacacaca gctcagcaag acaaatagaa cggtatagga caaagtattc
     6241 ttcaacactt tatgaaagac tggatcagtc agcaccttta catagttggc aaagccaaca
     6301 aattctttag tgggggccac catattccag tcaaaaaaac tcaaatagat cgtataaatc
     6361 aatggataaa aaacaaacac gcccaacaaa ataattgctg gcgccagata caaataacct
     6421 acatagcgct tcatttccga cagcctcctc atccttatgt caaaagcaga cgaccttgtt
     6481 tttcctttct tacaggcgct gtcgtctgct tgctttgctt atttgagtaa ctcattaatt
     6541 tccttttgag tagctttcat cgtttcattg acggaattac ctgataaaat tttatcccgc
     6601 atatccaaca atttttgttc agcttctaat ccagcatctc caggaaatga actccaactg
     6661 accatcttat ccatctgatc aattgctggt atcatcatct gattttcagc taagaaagct
     6721 tttaatccat tttgggcgtc tgcaacatct ttacgtggtg gtaaataacc agtaccttct
     6781 gtccatttag ctaaagattc aacactataa agatatttta agaaatccca agttgcttcc
     6841 tttttcttat ccgtttgtga tgtaacagct aacattgcgc caccagcagg tagtttaact
     6901 tttttccctt cccaacttgg tgactcaact gctttagcag taaaagtact atttttttga
     6961 atgttgtccc tttgagcaat agtggtatag accatcgcga cgttaccatc aataaagctt
     7021 tgcacacctt gttcccacgt agtgtgtaat gccgtctggt cttttaccat cctttgatat
     7081 aattcataag cagcgatacc atctttagaa gcaaaggtag ctttgccatc ttttattaca
     7141 cttgccccat tactttctaa aattgcttgt tgtgcccaat tatcagcagg ttcttgaata
     7201 tacacgccat actttccagt tttttctttt attatcttag aaaaagtttg aacttcttcc
     7261 caagtttttg gaccattttc atccaagccc gctgctttta atatatcttg atttaaatat
     7321 aaaaccggat tactaatgga gtaaggaata cccacttgtt taccatcttt ttcagacagt
     7381 gccatgatat tgtctaaaaa atgatcttta aaataatcag cattatccgg atccacttct
     7441 tctactaaag attgaggatt agtatatttg aaattttctg caaaataatc tttaaaagcc
     7501 catcccactt gtactacgtc cggtatttct ttggaggcaa cagaagcttg taaattttgc
     7561 atcaagcctt tatacatatc aggattatat tttgctacaa ccttcacttt gtcttgagat
     7621 tgattgtatt tatctactaa ttcattcact gtttttccac ctagggtatc cgcattaaca
     7681 tgccaatact ctatcgttac acgaccctcc gatttttttg ttgtcttggt attcccacta
     7741 ccacaagctg ccaaagagat gacacttaaa acagacacaa cccctataac aagatttttc
     7801 tttaacataa cattaacttc ctcctaattt ctttatggcc atttttctaa tcaagaaata
     7861 ttatgaacta ccaatgtaaa tttctagtta attttctgta aataattcga aatttgtgca
     7921 tttcggggta cagggtcact ggacatatcc atcaagctaa gaataaagcc cccatatggt
     7981 catgtatccc atctccaaat ctcttaactc ctctgttttg attataaaat agtttattct
     8041 gtgctccttt cttccataaa taggcccatg cacaacgtga ggttatatta taaacaccac
     8101 agcgcgcaag gtactcaatc gtttctagtt cattgactgt ccatacagaa aatgtcaatc
     8161 ctagctgctg ccccacttca cgggccaact tagttaacca tccttcatga atattgacga
     8221 tttgaacgct ttgataggct aatttctgta aggtatcgat caaatctgct tcggtaatat
     8281 ccccaagttt tttcccttcg aactgatttt caatattata aaatatatgc tttccccaaa
     8341 tcgcagccca agataattgt acatagccac taagaacaat ttgtggccag atttgatagg
     8401 tttgtgccag cgaaaaaacg gccatttcca atccctcttc ttttaaatca cagttaatgg
     8461 tcatggttgc cttctcattg gccactgctt gaaacacctg ctcaagggtt accgaatctg
     8521 ggacaacttc ttacctgtca tgggctagta ttagcgtacc atctgtttgc tgacgcacat
     8581 ctacttcaaa acaaagctgg cgatccaaac aaaagcgaag aaatgcccag ctattctcct
     8641 ccgtattgtc actaccagag tgtgcagtga tcatggtcat tttgacccct cctttaattc
     8701 cgtgattttc tcccacacgt ccactatacc gatgaagcgt taatttcttg ttaatttctt
     8761 gtaaattccc tgcgtttttt cagtaaacta gttgtaaatc ttgcatgctc cttcctacga
     8821 actcatgaat cgtcgatttt gcccttctag ctagtttcac caattaagta gttctacatt
     8881 ccccgctttt aacctactac accttttttt gttttcagct attccttctt cttgctgaaa
     8941 gtccttgagg ttctttctta caggatacca tcaaggtgtt caatggctta ttcccattga
     9001 atgaccgaga cttactatca gatttctgct cgttgaacca agtctttgat acaggatttc
     9061 ttccgtcatc ttctcacaaa taccaccttg cgtttctcta gcgattggtc gatgtgcacc
     9121 ctcccagtgg actgtcacta cccctttttg ctatgcctag cccacaaaaa aagctgtcct
     9181 cacagacag
//
LOCUS       DAYYCQ010000100         8954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000100 DAYYCQ010000000
VERSION     DAYYCQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.102"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(447..944)
                     /locus_tag="ACHAYF_002535"
     CDS             complement(447..944)
                     /locus_tag="ACHAYF_002535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8579912.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(984..1793)
                     /locus_tag="ACHAYF_002536"
     CDS             complement(984..1793)
                     /locus_tag="ACHAYF_002536"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8579913.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2127..2984
                     /locus_tag="ACHAYF_002537"
     CDS             2127..2984
                     /locus_tag="ACHAYF_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8579914.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2977..3879
                     /locus_tag="ACHAYF_002538"
     CDS             2977..3879
                     /locus_tag="ACHAYF_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8579915.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3912..4262
                     /locus_tag="ACHAYF_002539"
     CDS             3912..4262
                     /locus_tag="ACHAYF_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8579916.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4311..5234
                     /locus_tag="ACHAYF_002540"
     CDS             4311..5234
                     /locus_tag="ACHAYF_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8579917.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5312..6205
                     /locus_tag="ACHAYF_002541"
     CDS             5312..6205
                     /locus_tag="ACHAYF_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8579918.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6243..6806
                     /gene="amaP"
                     /locus_tag="ACHAYF_002542"
     CDS             6243..6806
                     /gene="amaP"
                     /locus_tag="ACHAYF_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8579919.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6820..7008
                     /locus_tag="ACHAYF_002543"
     CDS             6820..7008
                     /locus_tag="ACHAYF_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8579920.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7021..7923
                     /locus_tag="ACHAYF_002544"
     CDS             7021..7923
                     /locus_tag="ACHAYF_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8579921.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7955..8197
                     /locus_tag="ACHAYF_002545"
     CDS             7955..8197
                     /locus_tag="ACHAYF_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8579922.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8453..8824)
                     /locus_tag="ACHAYF_002546"
     CDS             complement(8453..8824)
                     /locus_tag="ACHAYF_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579923.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3116 a         1551 c         1738 g         2549 t
ORIGIN      
        1 cagttggtta gagcagacgg ctcataaccg tccggtcgta ggttcgagtc ctacaaggta
       61 cataagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca agtaaaaaca
      121 gcttcttatg tttttacttg gctgttctta ttactgcaga tgcaagcttt tgaataccgt
      181 ttaaggaaga ggttgtgaaa tcagcgtttt gacctgagta ttagcttaac aaacggaaaa
      241 atagttcctc atattttgcc gtttgttaag ctaataccga gggtcagctg attgaacacc
      301 atttaagaaa gaggtcgtga gcctgccgtt tcactctgtg tcataaaaaa gccaatctac
      361 tttgatagaa aaagcacgta ggactaaaac agttctgcgt gcttttttta ttgtgtgtta
      421 aatatcgtaa atgaagatag atatttttat gattcgacga tagagacgtt gaaatctttc
      481 aacagctcta aagcggattc tccgtgtttt ctactggaga ctaacattgg atcaacaaaa
      541 aagtctgctt caggaaaagc actctgcagg cctatcagat tagagagtac acacaagttt
      601 gtttcaatcc cgactacttc gatctctttt tgttcttctt tgctttcaaa taaagtttcc
      661 tggatttcag ctaaagtttc aggtggaatc gtaaagtaat actttttaat cactcgttca
      721 ttttcttgtg gagaaagaag agggatcaat tgcaaatctt ctcgttcttc ttctttgtcc
      781 ttcagttcaa ctggtatatc ccgtgtgaag agaatatatt cattattttg gcgtgcttta
      841 gctaatcttt gagcaatacg ttctgccaat tgatctgact ctggaatata aaattcgctg
      901 tttggatcaa gaatatgatt ttgcatatca ataataacta acattttctt tcctcctaca
      961 ttcatatcgt cattttattc tttttaatag aagtttttta aaatataagc aacaccagct
     1021 tcttcattcg tcagtgttac ttggtttgca atttctttta cttgtcgctg tgcattcccc
     1081 atcgcaatac tcaacccgct gtaattcaac atagcaatgt cgttttcttg atcgccaaaa
     1141 gccatgatgt cactcgattc gattccaagg taatccgcca gaaaaacaag tgaatgagct
     1201 ttagtgatac ctgtaggcgt gatttctaag aaatgcggat cagagaatac tgattggata
     1261 ccatctgctt gcagatctct ttgtagcttc tgtagttttt cttcactctt atctgttaat
     1321 gctaatttaa gatagctaag agaaggatct tccaacatat ttaaaatttg atcaaaagaa
     1381 tggttctgta aattgaaatg ttcataataa gggtcatctt tgactagatt tgattcatcg
     1441 aatgtcagct gatcatcttg ggtttctcgg ctagcaatga tggatatttt cgcttcttga
     1501 gcatagaaga aggcttttct cagtgtatcg ctatctattt ttgaacgtct gagaactttc
     1561 gccatttttc catccttcat ttcagcaatc agtgaaccat tggaagaaat caaatagcca
     1621 ccgattccta attcggaaaa aatatcttta gcagaaatgc tgttccttcc ggtagcgacg
     1681 gttacgattc cgccattttc atcaaatttc cgaagtgctt ggatattttc ttctggtatc
     1741 tttccatttc gatcaagcaa ggtaccgtct aaatcaatcg ctgctagttt cattttatct
     1801 tcctactttc tttttacata tctattttcc tgttacctta agattaaatg agaatttaaa
     1861 aaaatgaaac taacttgctt tgtcattatg aattttttca aaaaacattc ttaagaaaaa
     1921 ggtggctata tatagtagtt tttggtcaat acctttgaat aaaaagccat gaaaaacgaa
     1981 tagttagcgc ttaccgatgc gttttgtgat aaaaaaattt taaaatattc aatgaaaaca
     2041 aagcatatat tttgctttaa aaccaaatga ggaatattct agatgtagaa caggttagaa
     2101 aaagattaga aaaaggagtg attttgatgg agaagagata cggcgtagtt cctgtggtaa
     2161 ctgcttcaga cgaaaattat gctccttact tgagcgtgat gatcgcaact gcattagaaa
     2221 attgcaataa aacaagacgc ataaaatttt atgtgattga cgatggtcta tcagaatata
     2281 gtaaagaagg attggaagaa acggtaaata aatattcaag taatgcaagt atccaattcc
     2341 tgacagtgga aaaagatatc tacgaagact tcctagtcag tgaccatatc acaactaccg
     2401 cttacttaag aatttcttta ccaaacttat tagctaaaga ggattataag aaagtccttt
     2461 acttagattc agatgtgtta gtactagacg atatcgtaaa actatatgat gagccgttaa
     2521 atggaaaaac aatcggcgct attatcgatc caggccaagt gaaagctttg gaaagattag
     2581 gaatcgattc cgatgacttg tacttcaatt caggagtgat ggtgattgac attgatcaat
     2641 ggaataaaaa agaaattaca gaaaaaacta tccattacct cagtgaaaat ggcgatcgaa
     2701 tcatctatca cgaccaagat gctttgaatg ctgtacttta tgaagattgg gaacaacttc
     2761 atccaaaatg gaacatgcag acttcgctga tttttgaacg tcatccagca ccaaatgaaa
     2821 aatatgaacg tcagtataaa gaaggaaatg aaaaaccttc gatcgttcac tttacaggac
     2881 atgataaacc ttggaatacg ttgaaagatc atccgtatac caatttatac ttaaaaaaat
     2941 tagcacacag tactctaacg aaagtaggcg agatcaatga ataaaaaaga aatagcagta
     3001 gtcgcaagtt gtaacacaaa attcgtacca catttagctg cattatttgt ctcagtatta
     3061 gataattgca acccgtctaa atttgttcgg ttctatgtga ttgacgatga tatcgatttt
     3121 gaaagtaaac agcttttacg cttctcagtg aaaaacgcac gaatgaactc agatgtggaa
     3181 ttcttgaaaa tcaataaaga attctttaca aatgtggtaa tcagcgatcg tattccagaa
     3241 acagcttatt atcgtattgc gattccggaa ttgttccgcg gcacagaagt tgaacgtatc
     3301 ttatatatgg attgcgacat gatcgcttta caagatatct ctaaattatg gcgtcttgac
     3361 ttcggtgatt caatcgtagc agctgttgaa gacgctgggt tccatcaacg actggaaaaa
     3421 atgaaaattt cagctaaatc aatgcgttat ttcaactcag gtttgatgct gatcaatgtg
     3481 aaaaaatggt tagatgaaaa tattacgcaa aaagtattag atttcatcga acataatcca
     3541 gaaaaattac gattccatga ccaagatgca ttaaatgcta tcttacatga ccgttggtta
     3601 ccacttcatc cgcgttggaa tgcacaaggc tatatcatgg caaaagcaaa aaaacatcca
     3661 acagcagcag gtgaacgtga atacgaagaa actcgcaaca atccttacat tatccatttt
     3721 tcaggacatg tgaaaccgtg gagcaaggat tttgaagggc caacgaagaa atattacgaa
     3781 aaatatgcag ggatgacggc tttccgttgt gtagctaaat tccctaaata tccaaaatat
     3841 gcaaaagttc aaccaaaaac agatgtagtt gttaaataat agaagaccac aactgaagag
     3901 ggggagtagg aatggaaatt acaatgtcta aaaatgcagt agagacgtta attgaaaagg
     3961 ttggggaaaa tacaaaaatc gcactcgcgc ttatcaatga ttccgatcca tttctaaggg
     4021 acaaaggtgc ttttgcaaaa ggcagctttt ttcaaatcat tccttttgtt tcggaatttg
     4081 gagaatatgc gactaaaatc gagcatccat tattggacat ctatacatct aaattagagc
     4141 aaaattattt cggtaaacga ttgaacatgg acttcaacaa acagcttgac agcttttcat
     4201 tagaaaatga aatcgcagta ctcgattata atatcaaatt aaagaactgc ttcttcagct
     4261 aatagtttcg agaaagtcca aaaaggaggt atacattgag atactacttt gtggatgaag
     4321 aaaagaatgt aatagaaaca gatgaaaaaa aatataattg gctcgtactt gatagtgcaa
     4381 agcccgaaga gattgagcaa gcaatcgaag aattcgagct tcctgatgat attttcgtag
     4441 gaaccgaata tccggaagaa gtttctcgga tggagcgcct ttatgagacg aaactagagc
     4501 atccattttc tttggtagtg attaatctag atcagtcaga taatcgtatc gaaaaaaaat
     4561 taacgccaat atcttttgta ctttccgata atctcttgat tacttgtatc gatagaaaaa
     4621 ccgaatttgt ggatcggctg ctgacaaagc atggaaatca tttaaactcg tttgaaaaga
     4681 tcatcacgta tacattatta gatatttaca cccattacgt gaaagaacta agagagatga
     4741 aaaagcgaat tgacgctttg gataaagcag ctcgtaaaac aacggaaaat gaagaactgt
     4801 ataaacaggc agatcttgaa agagacatcg tatatatcga tcatacctta agagatcaac
     4861 gggaaacaat ggatcaatta tggaattctg aaactttcat caaacgttta gacgatgaac
     4921 agctactcta tgatgtccat ttgagacagc gacaaacaga aaaaatgatc gagatctatc
     4981 gagatttatt agagagtatt ggtgatctat tcaacgggat gatggacaat aatctaaatc
     5041 atatcatgaa atatctagat tcagctgcat taatcatttc cgttccggcg ttgttcgcag
     5101 ggatttgggg aatgaataca ggaggattac cgggtaaaag ttctgctcta ggctttatac
     5161 tcgtagtctt tggctctttg atagcagcag tagttttagg ttaccattta taccggaaag
     5221 attatacaaa ttaaagaatc tatttaaaac aggtatcaaa caaagaaatt tgttttggca
     5281 atacaattta aaaactagga ggaatacagc aatgacagaa ccaaaattaa tcgacccgcg
     5341 aaacttgtac catacggaga aatttcctaa acaagatcag gatacaccag ctttacagca
     5401 gaatatgatt cctgttcctg attgtggaga agaatcctat gaaggcaata atcaactaga
     5461 aaaccgccgt gtactaatca ctggtggaga ttcagggatc ggtcgtgcag cagcgattgc
     5521 atttgctaga gaaggggcag atatcgctct tcaattcttc ccaggagaag aaaaagatgc
     5581 agaggaagta gcaaaatacg tgaaagatgc aggacgtaag attgtcttac taccttttga
     5641 tcttcgtgac gaacaagcac ctaaagaaat catagataaa gccgtgagtg aacttggcgg
     5701 gctagatacg atggtactaa acgcaggaca gcaaatttct tgtcagtcta ttgctgagtt
     5761 accacttgag caagtcaaag acacatttat gataaacatc atcgctatgt ttgctttagt
     5821 gaaagaagct gttccgcata tcccagcagg aggagcaatc gtaacaacaa cttctgttca
     5881 agcattcaat ccaagcgaac atttattaga ttacgcagca acaaaagctt caatcgctaa
     5941 ctttacgatc ggtttagcaa aacagcttgc acctaaagga atccgtgtaa atggtgtagc
     6001 acctggtcca atctggacac cattacaact agacgaagga caaccttctg attcgatccc
     6061 tgaatttggt cagcaaagtt tactagaacg tgcaggtcag ccaggagaat tagcaccagt
     6121 ttatgttttc ttagcatcga ataaagcaag ttatgttaca gcccaaattt acggagtaac
     6181 tggtggagaa gcgatcaact tataagaagt tgatcgtttc acccctatga ggaggtgcga
     6241 aaatgaataa aggtgtaaaa acgatcggag tgatcggaac attacttttg ctatctgtgc
     6301 ttttgtttgc gatactatta agtgcaacct atccattgcc ttttcagcta gagaagtttc
     6361 gattcttttc catcactaat tcatacgtgc aacaatatgt attttggctt gccgttgcat
     6421 ttgctatttt gacaattatc acgttacttg ttttcctgtt ttatccaaaa acaatcggaa
     6481 actttctact gaaacatggt gatggggagt tgacgttgga taaaaaagca attgaaggaa
     6541 tggttcgttc tcacttgcat gaagatgaat tcatccattc accaaaagta aatgtcaaag
     6601 caacgaaaaa caagatcgcc gtcaatgtaa aaggggagtt gaaacgaact tcagcactca
     6661 ttggtaaaac aggaacattg atgaaagaaa tcgaaaatga agtacaacac atattgggaa
     6721 caaaagagcc agtgaaagta tcggtgaaat acacaggctt tgaagaacca gctaaacgaa
     6781 gcaatgaccg caaacgagta gaataggggg ggaccaaaaa tgaaagaact attttcgaca
     6841 tacaagctcc ctatcatctt tggggtactt ggattgatat tagccgtttt atggatcacc
     6901 gtgggctttt tcaagacctt attgatcttg atcatggcag cgatcggcgt cgctatcggt
     6961 ttttatctga aacaaacgaa aatcttagat cactatttta aatcttagga ggaaacatcc
     7021 atgaataaca ataacaaaaa taataacaat aaaatgaaca accaaaaacc agtgtctgca
     7081 catccagacc caacgacacc aaaccaatca aatggtgtga acgatgaaaa gaacaaaaca
     7141 gcaccagtat ctgcacatcc agatccagta gtccctaacg ttcaacaagg agtagaagag
     7201 aagaaacatg atgcagaaaa gcatgcacct catcatccag gaactccaga atctaaagtt
     7261 ccagaacaac atgatgcaat cgatccttct aaacctgtgg aagagcaaaa acatccttct
     7321 gaccatgcga aagcacaaga aaaacttagt tctgaccaca caaacggaca aggaaaactt
     7381 ggttctgatc atacaaataa ccaaggaaag catgattctg aacaggaaga ttcaatgcgc
     7441 ggtgagctga attatgaaga taaagttgtg caaaaaatca tcggaatcgc aattgaacaa
     7501 gtagacggtt tattatctgc aaacggtggt ttcttctcta atgtggcagg aaaacttgtg
     7561 aatacagata atgtaacggc tgggatcgaa acagaagtcg gtaaaaaaca agtagcagtt
     7621 gatatggatg tcattgtgga atatggcaaa gatatcgaaa aaatctttga agaaatgcaa
     7681 gaagtaatcg gcaaagaagt aaaaaatatg acacatttag aagtgatcga agtcaacgct
     7741 aacgtagtgg acatcaaaac aaaagaagaa tttgaaaaag atcaagaaac ggtgcaagac
     7801 aaagtaactg acgcagcgaa aaaaaccggt gaatttgctt cgaatcaaac agataaagta
     7861 aaatcagcag ctggaagcgg cgcacaaaaa gtaaaagaaa atacagaacc acgggttcag
     7921 taataaggaa gtaaaacatt aggaggaacg aaacatggga tttatttggt cacttatcgt
     7981 cggaggaatt atcggagcaa ttgcaggagc aatcacgaat aaaggttctt cgatggggat
     8041 tattgcaaat gtcgtagcag gtttagttgg atcagcactt ggtcaagcat tattaggttc
     8101 ttggggacca agtattgcag gaatggcaat cgtgccatca ttgattggtg ccatcatttt
     8161 agtactaatc gtatcattct ttgctcgaaa actgtaaaaa gacttttaaa aagaggtcgt
     8221 gaaaaagctg ttcttcatca agtaataaga acagccaaac aaaaatagct tctcatattt
     8281 ttcatttggt tgggcatatt atcgaagatg caagcttttg aacactgttt atacggaata
     8341 agaggctgtg acaagacttt tgtcacagcc tcttatttat gcgggcaaat tcccaattag
     8401 atttttaaaa gtttttcctt aaatgtgtct ttattgatac agagaagctt ctttaataaa
     8461 acaatgcctc cctaaatttt cttgagatag gaaaggtgaa catacaaaat aatacgacca
     8521 ctttgaaaac ccagatgatc cacaaatgga tcgtatgata ctgatgaagt aaaaagcaat
     8581 ggatcagaaa ctcaaagaga ttgaaaaaga caaggaagaa ataatatctc ttttttactc
     8641 ttgtctgttt tagccaataa tagctcgtaa agtaatctag acaaacaaat agaagtaaac
     8701 aaagtttcgt aagagcagca atcgtatcat gttgctctgc tagacgattg acgagcagga
     8761 aaaatgttgg taatggaata tagatcacga gtaatgaaag aaaaaagtaa acaataaatg
     8821 gcatactatt cctcctaatt gtgtgagttc gccctcattt taaaataacg atatttgttt
     8881 gtgaagcttt ttcgtgtcgt aacaaatgtt atctaatgta tttttaaata aataatcatt
     8941 attatcaaaa taat
//
LOCUS       DAYYCQ010000101         8717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000101 DAYYCQ010000000
VERSION     DAYYCQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.103"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(315..2696)
                     /locus_tag="ACHAYF_002547"
     CDS             complement(315..2696)
                     /locus_tag="ACHAYF_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8579924.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2686..3333)
                     /gene="recU"
                     /locus_tag="ACHAYF_002548"
     CDS             complement(2686..3333)
                     /gene="recU"
                     /locus_tag="ACHAYF_002548"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8579925.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3397..3936
                     /locus_tag="ACHAYF_002549"
     CDS             3397..3936
                     /locus_tag="ACHAYF_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8579926.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4026..4445
                     /gene="gpsB"
                     /locus_tag="ACHAYF_002550"
     CDS             4026..4445
                     /gene="gpsB"
                     /locus_tag="ACHAYF_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8579927.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4467..4834
                     /gene="rnpB"
                     /locus_tag="ACHAYF_002551"
     ncRNA           4467..4834
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHAYF_002551"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5043..6209
                     /locus_tag="ACHAYF_002552"
     CDS             5043..6209
                     /locus_tag="ACHAYF_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8579928.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6255..7751
                     /locus_tag="ACHAYF_002553"
     CDS             6255..7751
                     /locus_tag="ACHAYF_002553"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8579929.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7832..8515
                     /locus_tag="ACHAYF_002554"
     CDS             7832..8515
                     /locus_tag="ACHAYF_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8579930.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8524..>8717
                     /locus_tag="ACHAYF_002555"
     CDS             8524..>8717
                     /locus_tag="ACHAYF_002555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579931.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2747 a         1575 c         1845 g         2550 t
ORIGIN      
        1 aaaattattc aaaaccatat attgtatttt ttatttgata ataacaatgg gattattaag
       61 aagtggcact aaataaaaga ctagtgtgta tttagccgat ttaattataa acaagtaaat
      121 ggataaagta tatgtaatga taaaagaaaa atggattttc taatttttaa gctaaatgtt
      181 agatcacaca tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca
      241 tgatttttat ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg
      301 tggtttcttt tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca
      361 tttgcagcat ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa
      421 gacggtgtcg agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg
      481 cttgtttctg ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta
      541 gagctagagc tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg
      601 gaagatggaa tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg
      661 tagtacagtt caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc
      721 acgttagctg aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat
      781 tctgaagtga caggcgtcat ttttttattg taacctgtcc acacggaaat cgaataattt
      841 ggcgtatagc ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca
      901 tactcgtcat ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt
      961 tgcgcattcg taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa
     1021 gctgtttctg gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct
     1081 tggaagacga ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc
     1141 gcataggctg ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc
     1201 gtattactag agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt
     1261 aagaaagaag ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta
     1321 cgggataagt aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt
     1381 cctacaggag tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta
     1441 gaatatttca aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca
     1501 aaatcacggg aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga
     1561 cgtccgccaa tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact
     1621 tgcatctcgt cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc
     1681 ttttgggcgt ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta
     1741 tctgtttttt cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta
     1801 ttgtcatcgg attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga
     1861 tcatattctg tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt
     1921 cttttcttcg cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc
     1981 ccagcaagca atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc
     2041 tcagaagctg tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag
     2101 gtcaagatct cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg
     2161 gccttgcgtt tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat
     2221 tgctgtgtca aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa
     2281 ccgatgatcc gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac
     2341 acgatcgcat cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct
     2401 gcgcctaaat cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg
     2461 tctaattttt tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca
     2521 cctgctaaaa agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat
     2581 ccttttttat gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc
     2641 tttttaggag taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt
     2701 gctttctcct tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat
     2761 ccgcggagaa ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag
     2821 tgatttgcgt cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa
     2881 ataaaaacag cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc
     2941 gaggcatgct ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt
     3001 tttgttcttg gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata
     3061 gtctgtagtt gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg
     3121 atagtctact ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg
     3181 ggaaagataa tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc
     3241 gaattcagtg atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata
     3301 ataaggttgg ccatttggat agcgcaatac catctttaat cacactccta ggccacatta
     3361 tagcaaaaaa gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat
     3421 gtgtctgggt atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa
     3481 gttgttaaaa atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg
     3541 gtacttgtca gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg
     3601 aaagaagaat atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat
     3661 aactggaatg aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc
     3721 gaagctgtta gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt
     3781 atacttgagc atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg
     3841 aagtattttt ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg
     3901 atcagtatgg acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa
     3961 cttttttcct atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg
     4021 taaatatggc gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa
     4081 aaatgcgcgg ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt
     4141 atgaaactta cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa
     4201 aagtagctca attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg
     4261 caccaaagag cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac
     4321 gtgaagtatt tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta
     4381 atccaaataa ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat
     4441 tttaattttt gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc
     4501 tgaggaaagt ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc
     4561 ataagctaag gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct
     4621 taagtatcct tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg
     4681 cggtaaaccc ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa
     4741 gaacaggaca aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc
     4801 tggaaaagca aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc
     4861 gtgacaaaag taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac
     4921 ttcagatgaa tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg
     4981 agtaaaagtg tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt
     5041 gtatggaaaa aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac
     5101 tggttggtaa agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac
     5161 gttttgaagg aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc
     5221 gagtaaaaat cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg
     5281 taaaagcatt gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa
     5341 aatcgatcaa gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa
     5401 tcgttgaacg tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg
     5461 gcgcacattt tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata
     5521 cgacaggacc aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga
     5581 aagaaaacat ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt
     5641 atgatcctgt atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata
     5701 tcgcaccagg attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa
     5761 tcatggaaaa tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata
     5821 ttactggttc agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa
     5881 tcggattagg ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata
     5941 aagaatacgg ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa
     6001 ctgttcggaa attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta
     6061 aatatatctt gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa
     6121 aaagaaaatt gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc
     6181 cgcctagaaa aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt
     6241 tggaggaata gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc
     6301 tacaacaagc tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta
     6361 gtgcataccg aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg
     6421 gtccaaaaat caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag
     6481 ttggaactgc cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg
     6541 aagatcttat gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag
     6601 ctagagaaac acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa
     6661 caaaacagtt gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga
     6721 atcaatacga acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag
     6781 aaggaattct ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact
     6841 ttttaaatcg gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac
     6901 aacttggtta tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg
     6961 gaattaatcc gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg
     7021 ctgtttttgg agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata
     7081 aatatgcgta tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt
     7141 tgtttaacga gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct
     7201 ttcaagaatg tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat
     7261 tgaaccatac aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc
     7321 atatcatcat tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg
     7381 aactacctga aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg
     7441 atgtggaagg catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct
     7501 cttatgcatt agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag
     7561 atgtggaaaa tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc
     7621 atatccatca atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg
     7681 aaccgcttaa tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc
     7741 aggtaaaata aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt
     7801 cagctattta cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta
     7861 tagtatggac caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt
     7921 tgtcaagtta gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct
     7981 tgcagcagct tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc
     8041 aaagcaacag cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat
     8101 gattttttcc attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact
     8161 atctctggaa ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg
     8221 cattttgcgt actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact
     8281 gtatccgcaa acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca
     8341 tttttatgaa aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga
     8401 agccaaacga agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc
     8461 tattgacgat gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag
     8521 gttgtgacag aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc
     8581 tggaacactg tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca
     8641 atttcaaaaa atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca
     8701 ggagaacacc gtttatt
//
LOCUS       DAYYCQ010000102         8586 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000102 DAYYCQ010000000
VERSION     DAYYCQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.104"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1511)
                     /locus_tag="ACHAYF_002556"
     CDS             complement(45..1511)
                     /locus_tag="ACHAYF_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8579932.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="ACHAYF_002557"
     CDS             1898..2494
                     /locus_tag="ACHAYF_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8579933.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="ACHAYF_002558"
     CDS             complement(2552..3034)
                     /locus_tag="ACHAYF_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579934.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="ACHAYF_002559"
     CDS             complement(3140..3337)
                     /locus_tag="ACHAYF_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579935.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="ACHAYF_002560"
     CDS             3632..4390
                     /locus_tag="ACHAYF_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8579936.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="ACHAYF_002561"
     CDS             4450..4878
                     /locus_tag="ACHAYF_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579937.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="ACHAYF_002562"
     tRNA            4924..4996
                     /locus_tag="ACHAYF_002562"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="ACHAYF_002563"
     CDS             complement(5146..5517)
                     /locus_tag="ACHAYF_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8579938.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="ACHAYF_002564"
     CDS             5732..5917
                     /locus_tag="ACHAYF_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579939.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="ACHAYF_002565"
     CDS             complement(6000..6188)
                     /locus_tag="ACHAYF_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579940.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="ACHAYF_002566"
     CDS             complement(6212..6475)
                     /locus_tag="ACHAYF_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579941.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="ACHAYF_002567"
     CDS             6633..6890
                     /locus_tag="ACHAYF_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8579942.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="ACHAYF_002568"
     CDS             complement(6961..7152)
                     /locus_tag="ACHAYF_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579943.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="ACHAYF_002569"
     CDS             complement(7293..7613)
                     /locus_tag="ACHAYF_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579944.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="ACHAYF_002570"
     CDS             complement(7636..8070)
                     /locus_tag="ACHAYF_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8579945.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..>8586
                     /locus_tag="ACHAYF_002571"
     CDS             8405..>8586
                     /locus_tag="ACHAYF_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8579946.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2719 a         1694 c         1361 g         2812 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctgaggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattc
//
LOCUS       DAYYCQ010000103         8215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000103 DAYYCQ010000000
VERSION     DAYYCQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.105"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHAYF_002572"
     CDS             476..1339
                     /locus_tag="ACHAYF_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8579947.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHAYF_002573"
     CDS             complement(1468..3090)
                     /locus_tag="ACHAYF_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8579948.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHAYF_002574"
     CDS             3339..3860
                     /locus_tag="ACHAYF_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8579949.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHAYF_002575"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHAYF_002575"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8579950.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHAYF_002576"
     CDS             5941..6759
                     /locus_tag="ACHAYF_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8579951.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHAYF_002577"
     CDS             complement(6862..7059)
                     /locus_tag="ACHAYF_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579952.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHAYF_002578"
     CDS             7228..7806
                     /locus_tag="ACHAYF_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8579953.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHAYF_002579"
     CDS             7901..8038
                     /locus_tag="ACHAYF_002579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579954.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2465 a         1480 c         1619 g         2651 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaat
//
LOCUS       DAYYCQ010000104         7979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000104 DAYYCQ010000000
VERSION     DAYYCQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.106"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..823)
                     /locus_tag="ACHAYF_002580"
     CDS             complement(<1..823)
                     /locus_tag="ACHAYF_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8579955.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAI"
     gene            complement(1047..2987)
                     /gene="thrS"
                     /locus_tag="ACHAYF_002581"
     CDS             complement(1047..2987)
                     /gene="thrS"
                     /locus_tag="ACHAYF_002581"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8579956.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3324..3503)
                     /locus_tag="ACHAYF_002582"
     CDS             complement(3324..3503)
                     /locus_tag="ACHAYF_002582"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8579957.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3550..5373)
                     /locus_tag="ACHAYF_002583"
     CDS             complement(3550..5373)
                     /locus_tag="ACHAYF_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8579958.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5651..7930
                     /gene="helD"
                     /locus_tag="ACHAYF_002584"
     CDS             5651..7930
                     /gene="helD"
                     /locus_tag="ACHAYF_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8579959.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2330 a         1702 c         1449 g         2498 t
ORIGIN      
        1 ggattgcttc tcgtgttttt ttcacttttt cgctaagctt ttcgttttta gccatataga
       61 aataaaaaat gatatcgatc gtagcaaatt gggccaaaag tgaattagtt gccgcacttc
      121 gattcgtgat ttcttttggt ctagatgttt gtacgagtac atcggctatc ttgctaagcg
      181 ggttatttcc aaattgcgtc aacgcaatga tttcgatatt catgcttttc gccagctcgg
      241 ctaatgcgac tacatctgca cttctacctg agtttgaaac aagccaaagc actttctttt
      301 tctcattgct gacaagttgc gggagcaaga cgtggatatc tttttcaaaa atgattggct
      361 taccgacacg agaccatttt tgcagaatat cttctactac taaagaagag gcgccaaccc
      421 catataaaaa aacagtctct gccgattcca aacactctac gactttttgg atcgtttctt
      481 cttctagaat atctactgtt tcttttatcg ctacttgtgc attgttacca agtttgtttt
      541 tgatagagta aaaggactca tttgcttcta tatcactaaa accttcatcg attccagtct
      601 gtagttctgt tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg atttttttgg
      661 aaaaacgtac aactgtcggc gcactcaacc cggaagcttc tgctaattca ttagctgtca
      721 ttgtagggac cttctcaata ttaccgataa tgtagttggc caactttttt tctgctgaag
      781 aataatcttc catcatactt tcaatacgac taattacgct cataagttcc tccacccttt
      841 taatgatagt ctatgtaagt ataccatatc agacccttat tcacggtcgt atactggata
      901 aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc catcaatccc acagattcta
      961 ttgagcatct gtgtctttga ggaatacctg ttatctattg ttcggattgc tttctatcta
     1021 ctactttttt acaggaaatt tacctattag ttttctctgc tgtaattttt gacttctgct
     1081 tccatagcat caataaagat attcaaatct tctacagctg tttctttact tccataacga
     1141 cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa caactaattg gtaagggatc
     1201 ttttgtgttt gagaggcacg tatcttatag cccatttttt cattacgatc atcaacttct
     1261 acacgcaacc ccttcatttg caaacgttct ttgatttcat aagcataatc gccatgtgca
     1321 tcaacagaaa cagggatgat tgttgcttgt ataggtgcca accatgttgg aaaagcgcct
     1381 ttgtatactt ctgttaaata agcgacaaaa cgttccattg tagaaacaat tccgcggtgg
     1441 ataactactg gacgatgcgt attttccccg tcttctccaa catatgtcaa atcaaagcgc
     1501 tctggcaata agaagtctaa ttgtatagtt gataatgttt cttctgtacc aagagctgtt
     1561 ttgacttgaa catctagttt tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa
     1621 tctagttcta attcatccat agcggctttc agcattgctt gggctttttc ccacattgcg
     1681 tcatcatcaa aatatttatc tgtattgttt ggatcgcgat agctcaagcg gaaacgataa
     1741 tctgtaatat tgaagtcagc atatactgcc accatcaatt caagcgtacg tttaaattca
     1801 tctttgattt gatcaggacg aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca
     1861 cgttgtaaac ctgacaaagc acctgatttt tcatagcgat gcatcattcc taattcagcg
     1921 atacgaattg gtaattcacg ataagaatgg atcgtgtttt tgtagaccat catgtgatga
     1981 gggcagttca ttgggcgaag aacaagcatt tcgccatctc ccatatccat tggcgggaac
     2041 atgtcttctt gataatgatc ccagtgtcca gatgttttgt acaactcaac atctcccatt
     2101 attggtgtat atacatgttg gtaacctaga ctaacttctt tgtctacgat ataacgttcg
     2161 atcgtgcgtc ggattgttgc cccttttgga agccagaatg gcaaaccaga acctacttct
     2221 ggggaaacca tgaacaagtc aagttctttt cctagtttac gatggtctct ttcttttgct
     2281 tcttcacgga gacggatata ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat
     2341 acacgttgca tcatttgatt gtttgagttt ccacgccaat aagcacctgc cactgataat
     2401 aatttaaata cttgtatacg gccagtggat ggaacatgtg gtccacgaca caaatcgatg
     2461 aattcgcctt gttgataagc agtaatcact tcttcttctg gcaattcact aatcagttct
     2521 actttgtacg gatcacttgc aaaaatttct aatgcttcct gttttgaaag aacacggcgt
     2581 tcgatcggca gattttcttt gacgattttc atcatttctg cttcgattgc cggcaaatct
     2641 tctgcagtta cttggttttg tccattatct gtatcgtagt aaaaccctga atcgatagct
     2701 ggtccaacgc caaagtggat atttggatag aggcgacgca tggcttgggc catcaaatga
     2761 gctgctgaat gacggactaa gccaagtgca tcttcatggt ctggtgtgat gatctcaata
     2821 cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac tttaccagct
     2881 aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca
     2941 gactcaaatt ctttgactgc accatctgga aaagtaattt ttatcataat agtttcctcc
     3001 ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct
     3061 tgaaaagaga tactaggacg aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg
     3121 ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc
     3181 atcctttggt tttctcctgg aaaagtttca gcctagcttt tcctctctta tcgaagactc
     3241 tcaaattcag ttggctttct catcaatcaa ttatgtgttt atttaaccac taataaatag
     3301 aaaaatcaag tgggaaacta aacttatttt ttccattccc catttacacc gtatgttcct
     3361 tttttcatct cattgatgat gacatggatg tgttcttttg gtgcctgcgt gtttttatga
     3421 acagcttccg tgatatcttt catcatattt tctaattgtt ccggactgcg accttctact
     3481 aattcgacat gtacgaatgg cataatgctc tctcctcttt ctgatcgttc ttttttcaac
     3541 tcgattctat catactatgt ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt
     3601 ttttgctctc aaggcactca aaatagagac cgtatccacg acttcttgca aaacagctcc
     3661 gaataatgcg gggattagtc ccgtacttgc aattaacatc aaaaaaatac aaataaagat
     3721 gccaatcagt acagactgtt tcgctattct catcgtatct cttgaaatca ctactgcttt
     3781 tgctacacgc aagagatcat ctttcaagat gaccgcatct gcactttcac tagcagctgt
     3841 agaaccatga gcccccattg caataccgat atctgcagca gcaagtgctg gtgcgtcatt
     3901 cacgccgtct cctaccatcg caacaggtct ttgatcttta gggatacttt ctaaaatggt
     3961 cagtttatct tgaggcaagc tacgggcatg tacagaatct aattggactt tttctgcaat
     4021 cgtttgagca atggcagatc catccccggt gatcatcatc aaatttttca catcaagttt
     4081 tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt
     4141 tcctgcaaaa gatcgatcaa ttgttacata gattgctgta ttggacgttt ctatttcttc
     4201 agctgaaaca aaagaggcac gtccaatctt tacttcatga ttattgacta ctgcttggat
     4261 tccttgaccg gtcacttctt tcagttgact aacaggatac agttggctgt ttttcacgtt
     4321 gttcactaac gaacgagcta agatatggct agattcctgt tctgcactag ctgcatacag
     4381 aagtagctct tcttttttta ttccattgac tggctgaatc gcctctactt ccaatatccc
     4441 tttggtaagc gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat
     4501 cgttgttcca gtttttacaa cgatcccgtt tttacttgaa cggctcattc ctgcaactaa
     4561 agcaatgggc gcagctagaa tcagtggaca aggtgatgcc actaccagta cctcagcaaa
     4621 tcgtaaaggg tcttttgaga accaccaaga aataccaccg atcaaataag caatcaacgt
     4681 gaaagggact gcataacgat cagctaaacg gacaaatcgg gcaggctttt cttttgattc
     4741 ttcgactagt ttaataatca tttgatactg gctgtctgcg gcacgttttt cagcgatcat
     4801 ttggatcgat ccgtctccat ttaaagaccc tgacataatc tgatctccag gtttcttggt
     4861 ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc gaagattctc ctttggtcac
     4921 tcgaccatct gcgggtacaa gttcgccagg cttgactagt aactggtcat caatatttat
     4981 ttcatcgatt actatatcct gcaattttcc ttctttgaat cgatgggcaa ttcttggggt
     5041 gttatctaac aaagactgca gttctttacc cgctcgttta ttcgcgtaat cctctagact
     5101 atctccgccc gtaagcatga tcaatatcat caaactagcc cagtattctt tgacggctaa
     5161 tgttgcaagg attgcggtaa tcgctaatac atctacgcca tatcttcctg aacgtaacgt
     5221 cttgatcatt tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc
     5281 aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat aaagctatcc caccaataag
     5341 caaagtaagt aaaaattttt tgaaatgtgt cattgttttt tctcctcttt tatgtgactt
     5401 agagaggtta gtaataaatt tccagctgcc tctttcatct taagcatagt ccttgactag
     5461 cactttttaa aacaataggc cttaattgaa aacaactatc actatcagcg tttccaaaat
     5521 tcatcagttc tacaaacaaa aatattcagc aaggattact ttttcgattc atcagttaaa
     5581 aagtaccttt tttcgaaaaa ctatgctata cttttaattc gttatttacg gcggaaggag
     5641 caaacatgcg atgaatgaga aacaactaga acaaacgcac gtggatgaaa cgatcgaact
     5701 aatccagatc gaacaaaata tgctggatac aaaacaacaa gaattgactc aggaaatgaa
     5761 tcattcgaca aaagacacag ctaatcacaa gattcgagct ggttccaatg aagcttttta
     5821 tgaatctgct gttgaatatc gccaacatga gcaggaattg cttttgaaat accatactct
     5881 tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc
     5941 ccgtatcgac tttaaagaag accgtgaaac agaaacatta tatctaggta tcgcttcttt
     6001 aagagatctg gcagaagaaa caatcgttat tgactggcgc gcaccaattg ccaatcttta
     6061 ttatgaaggt gagattggtc ccgcatttta tgaaactgaa acagatagaa tagatgtcga
     6121 tctgcagttg aaacgacaat ttaaaatcca agaaggacgt attttatcta tggtagatac
     6181 gtcggaagta atcaatgatg actttttgct agagatattg gatgaatcat caagcaacca
     6241 tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat gcaatcattc gggacacaac
     6301 aagtaaagta atgctgattg aaggaatcgc cggaagtgga aagacatccg ctttgcttca
     6361 acgtattgct tttctgttgt atcataatcg caagtggctc gaggctgaaa acgttcttct
     6421 attttcaccc aaccatttgt tttctgatta catctcgact gtattgcctt cattaggtga
     6481 aagcggcgtg cctacacaga cattcaaaaa ttatttggtg cagctgctgc cagagttttc
     6541 tttactagat gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag atccgataca
     6601 agtgatgaaa agcggattga ccttagtcga tcagattgat cgctatatcc aaggcatcac
     6661 aaactacggt cccttgttcc gtgatatgaa aataaatggc cgtacgatcc tatcaaagga
     6721 atcgattcgc caatggtata aagaaacaaa tgaacaatta cctttacatc atagactttc
     6781 tctattacaa acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca
     6841 gcaatgggtc aaagatctgg cggaagaaca gctccaagaa ctatacgcaa cagatccaaa
     6901 tcttgaatat acagaacaaa aggagcgttc attacgtaaa aaactggcca accaaatcgt
     6961 acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg tcaatataac
     7021 gaaacaatat ctccactttc tacaaactgt gccactttct cttctaacac gacataaaat
     7081 cagcgagacc aggtggcagg aacatttgac aactgttcgt acacacttgc gtcaaagaga
     7141 attgcgtcaa gaggatgctg tactattctt tttactgatg cgccgaacat atccagtgac
     7201 ggttacacac aaagctcgct atatctttat tgatgaaatg caggatttcg caccatcaca
     7261 agtggcattg ctgcaagcct tgttcccaaa agcaaatttg actttctgtg gagatctaaa
     7321 tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg gaagaccttt tcgtggaccg
     7381 tgaagtcact cgtttccaat taacgacaag ctatcgctcc acaaaagaaa tcactgattt
     7441 tgctaatcat tttattgtga aagaacatca agtagaaaca acagcaagaa aaggagacct
     7501 tcctttgctc atccaaggaa caacagatga tatcaaattg aattggttag cagataccat
     7561 tcgaacgact cagggaaaag cccggtattg gcgtacagca atcatagcta aaacacaaga
     7621 agaatgcgaa gctctttatg ctcaactgcc agacgagttg aaacaacaag tccagctgat
     7681 tttaaatgag agtgacttta tgaagcgttc cattatttta cttccagcct ttttagcaaa
     7741 aggtctggag tttgaccgag ttttcttgtg ggatgtagat gcagaacatt tttcttctca
     7801 gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg atgcatgaat tgaccctttt
     7861 tacctccaat aatgaaagtc ctttgttaaa caatatggat atttcatctt atgagaaaaa
     7921 agtactgtaa gtaaaaagac aactgctatc attaatgtta gcagttgtct ttttattta
//
LOCUS       DAYYCQ010000105         7941 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000105 DAYYCQ010000000
VERSION     DAYYCQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.108"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..199)
                     /locus_tag="ACHAYF_002585"
     CDS             complement(<1..199)
                     /locus_tag="ACHAYF_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579960.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="ACHAYF_002586"
     CDS             complement(362..1996)
                     /locus_tag="ACHAYF_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8579961.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="ACHAYF_002587"
     CDS             complement(2000..3373)
                     /locus_tag="ACHAYF_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8579962.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="ACHAYF_002588"
     CDS             complement(3389..3628)
                     /locus_tag="ACHAYF_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579963.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="ACHAYF_002589"
     CDS             complement(3810..6485)
                     /locus_tag="ACHAYF_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8579964.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="ACHAYF_002590"
     CDS             complement(6668..7591)
                     /locus_tag="ACHAYF_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579965.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2328 a         1564 c         1201 g         2848 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ctagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactagat tctataacat tagaagaaat tttaaatacc tttgataaaa
     7921 aatattgatg ttttttctct c
//
LOCUS       DAYYCQ010000106         7720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000106 DAYYCQ010000000
VERSION     DAYYCQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.109"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHAYF_002591"
     CDS             <1..107
                     /locus_tag="ACHAYF_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM8579966.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHAYF_002592"
     CDS             complement(176..904)
                     /locus_tag="ACHAYF_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8579967.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHAYF_002593"
     CDS             complement(917..2197)
                     /locus_tag="ACHAYF_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8579968.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHAYF_002594"
     CDS             complement(2338..2940)
                     /locus_tag="ACHAYF_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8579969.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHAYF_002595"
     CDS             3185..3391
                     /locus_tag="ACHAYF_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579970.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHAYF_002596"
     CDS             3703..4422
                     /locus_tag="ACHAYF_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8579971.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHAYF_002597"
     CDS             complement(4503..5186)
                     /locus_tag="ACHAYF_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8579972.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHAYF_002598"
     CDS             5405..7591
                     /locus_tag="ACHAYF_002598"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8579973.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2770 a         1301 c         1454 g         2195 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc
//
LOCUS       DAYYCQ010000107         7601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000107 DAYYCQ010000000
VERSION     DAYYCQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.110"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHAYF_002599"
     CDS             complement(138..428)
                     /locus_tag="ACHAYF_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579974.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHAYF_002600"
     CDS             complement(546..806)
                     /locus_tag="ACHAYF_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579975.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHAYF_002601"
     CDS             complement(943..1356)
                     /locus_tag="ACHAYF_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8579976.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHAYF_002602"
     CDS             complement(1812..2297)
                     /locus_tag="ACHAYF_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8579977.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHAYF_002603"
     CDS             complement(2436..2651)
                     /locus_tag="ACHAYF_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8579978.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACHAYF_002604"
     CDS             2875..3675
                     /locus_tag="ACHAYF_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8579979.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACHAYF_002605"
     CDS             3694..5562
                     /locus_tag="ACHAYF_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8579980.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACHAYF_002606"
     CDS             5555..6046
                     /locus_tag="ACHAYF_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8579981.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACHAYF_002607"
     CDS             6034..6588
                     /locus_tag="ACHAYF_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8579982.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="ACHAYF_002608"
     CDS             6606..7601
                     /locus_tag="ACHAYF_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8579983.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAYYCQ010000108         7539 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000108 DAYYCQ010000000
VERSION     DAYYCQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.111"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1965
                     /locus_tag="ACHAYF_002609"
     CDS             <1..1965
                     /locus_tag="ACHAYF_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8579984.1"
                     /translation="RFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAFFYCFTFV
                     QIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFWYALTATL
                     IINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVLLIVGGLILLLLLITS
                     IIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLFKVNKTFF
                     NQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQTRSDWLN
                     NQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGGTANMEWE
                     GLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRRKQVFDKF
                     GFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQLSTMQNHM
                     PYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKIDKPITFVF
                     YGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNFGAMALEH
                     ANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEKNLTEKQK
                     EILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2025..2741)
                     /locus_tag="ACHAYF_002610"
     CDS             complement(2025..2741)
                     /locus_tag="ACHAYF_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8579985.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2968..3510)
                     /locus_tag="ACHAYF_002611"
     CDS             complement(2968..3510)
                     /locus_tag="ACHAYF_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8579986.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3515..3742)
                     /locus_tag="ACHAYF_002612"
     CDS             complement(3515..3742)
                     /locus_tag="ACHAYF_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8579987.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3821..5701)
                     /locus_tag="ACHAYF_002613"
     CDS             complement(3821..5701)
                     /locus_tag="ACHAYF_002613"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8579988.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5890..6504)
                     /locus_tag="ACHAYF_002614"
     CDS             complement(5890..6504)
                     /locus_tag="ACHAYF_002614"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8579989.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6646..7206
                     /locus_tag="ACHAYF_002615"
     CDS             6646..7206
                     /locus_tag="ACHAYF_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8579990.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         2300 a         1507 c         1236 g         2496 t
ORIGIN      
        1 cgtttcatcg aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa
       61 agaattcatt ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc
      121 tttaccttcg tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag
      181 gttgccctaa aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt
      241 atgggatttt tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct
      301 accttgatca ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa
      361 gagcctgtct ttccttcaga tttaaaaatg ctgaccggat tgtcagaact attatcgatg
      421 gttagcccag tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt
      481 attattgtac aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga
      541 tttatcagta tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa
      601 aattcgcctt ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag
      661 caaggcgccg taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa
      721 atcatggaag aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac
      781 aacacaaagg cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc
      841 gttctcaatt taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct
      901 acagacccta ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta
      961 tccgtcggtt atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt
     1021 atcagtaacc tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa
     1081 ataagtccaa atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct
     1141 gctctttatc gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta
     1201 gacagtccgg ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat
     1261 gattcagcat acaaagaaac cttgaaagct ttacaaagca atacggaaac gtcacaattc
     1321 atccaattat ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac
     1381 tacactgctg aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg
     1441 caaggccttc actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc
     1501 gataaaccga ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat
     1561 gattcgaaaa aatatggatt agaacagcac cagacagatt atttcattta taacaacaag
     1621 tatagtcgtg aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt
     1681 gctatggctc ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa
     1741 gtatctaatc atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt
     1801 aatggaaaac aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact
     1861 gagaaacaaa aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt
     1921 aaccagtaca gtgcaacatg ggcgacacaa aaaataaaag actaacaaat aaactgagca
     1981 aggggttgtg ataaaagaca tgtcacaacc ccttgtttat ctcattaatt tgcttgtcca
     2041 taaacgattt gattaaagcc atttttcaat tcattattcg ctgtctcgtt gaagtaaaga
     2101 taattatcgg gattcccctg attttgttgt gtcaccaata cttttggctg attttgctga
     2161 aacccgaata gatagacgtg ctttttcaga tatggttctg tctctgcatc ggaatagacg
     2221 gctgtgattt gatagtctgc ttgacctgtc ccactttctg accattctgc agaaacaggc
     2281 actccaccaa tagccatctt ccactccccg ttcaaaatag cttgagggac tttaagacca
     2341 tataagtcca cactcatttg attcgtataa cttttgtatt gttgtcccat tgtttggccc
     2401 catttggtca taaatgcctc taattgtgcg gatttgtctg cattccacag tgtttgttca
     2461 acttgttcgc tgctttctga agaggaagat gaagcctcta cagaagaact tgaagtagat
     2521 gtttgagttg tttcagtcgt tttggaagac tctgacgaag tagaggtatc tgctgtttct
     2581 gtacttgctg cagttgactt gctctctgtg ctagttgttg tagtagttgc aactgttgct
     2641 ttcgctgttt cttttgttga ttcttttttc gattcattcc cgttggtaca accagctaac
     2701 aaaaaagcta gaccaattgt tactaaaatc ctttttttca attaaaaaac tcctttcgat
     2761 tactcccact tctttattct atcaaataag caatgtgatt ttattgattt ttcgtatttt
     2821 ctcatcaatc tttcaaatct tctaaaaact gagaagcacc cgctgatgtt gtctgtaata
     2881 catcagcggg tgcttctttt attttaatct tcgtgatttt ccaatttacc tcacgcttta
     2941 tatttgtatt tgaagaaaca tagactttta gtcatcatcg tcttcctcat ccaaatcttg
     3001 tcggacagtt tttccttgga gtctttgtac ttggtagatg atttctttat tgaaacttaa
     3061 aagttcaaca agcaattgtt gcaagccata tttttcttct ttttcagcta atttgattgc
     3121 ccgtgtgata aagacatttt ggaaatcaaa atcttttgca aagttttcca acattgttga
     3181 ggctggtaaa tatttgtttt gaccatgttc gtaaattttt ccataacgca tgacttcttc
     3241 taaagtagac ggaacgattt cattttcaat cactaactca tgagagattt tattgaacca
     3301 tctcgcttct tgatcagcat attctgaaaa aagggtactt aattcttgcg aagatgtacc
     3361 caaaacatac aattgtgctt ggatcaattt catttcttct aatttaaaat ttgctaatac
     3421 atgccccacc atggcgccag cagttggttt atgatggtcg atatctgctt gttttgtttc
     3481 ttgaacaaat ttttcttcca ctatattcat tgatttacgc ctcttttact tttgctgatt
     3541 ggacttcata acctaatttt tccacaactg cagctaattg ttcagggtgg acttcatctg
     3601 gatcaaagtt tgctttgatt ttgctcgcat taaacaaaac tttcgtattg cttaccccag
     3661 gctgttgaga aaccgcatgt tcgatttttt gcatacagct tggacaagct agtgtatcta
     3721 attgcataat aatttttttc atcgtagttc ttccttcctt tttcttgata gtcttattct
     3781 agattacagc ttccataaaa aaattgattc ttatcaagtt ttacgatttt tcttctttgc
     3841 tttcgtgtcc aaaatacaat aaacgcatcg cgttaaagat aacgatcaag atactagctt
     3901 cgtgaacgaa catcccacta gccatgtaaa taaacccggc aaataagccg ttgagtaaaa
     3961 ataagacgac accaatggca atcactacat tttcacgggt atttaaaaca gttttcttag
     4021 ccaagcggta ggcatgaacc aacgattcaa aattagattg catcaagaca acgtcactcg
     4081 tttcgatagc aacatccgtt cctgatccca tagcaatccc gatatcagcc gcagccaaag
     4141 agggagaatc attgattcca tctccaataa aagcaacgcg caatccttgt tcttggtatt
     4201 ttttcacaaa ttgaaccttt tcgtctggta ataattcagc atggacttca tcaatgccta
     4261 acatatcagc tacataatca gcagtcatct gattatcacc tgtcagcatc actaactgtt
     4321 ttgctcctgc ttgtttcaac ttagctaatt gttcagcgac ttctggtcga atcacatctg
     4381 acacacctat gatttgagtg atttttttgt cgattgctac aataatgacc gagctgcctg
     4441 tttgttgtaa ttgatgtaaa tcttgtactt gctttggtgt gagagaaagt ttgtgagcgg
     4501 caatcaattt ggtgttgccc gcacaaactg tatgaccatc aatctcagct gtgattcctt
     4561 gtcccttgat tgtctgatta tctttgactg ctaattgttg aaaatcaatg tctttctcct
     4621 gagcataatg gatcacggcg cgccccagag gatgatcgga taacgtttct aatcgagcca
     4681 ctaaagctaa gctgtcccct tcatcctctg tatagttctt gatagtggat acagcagtag
     4741 ttcctttggt caatgtacca gttttatcaa agacaaaagt atcgattttg gaaaaagtat
     4801 ccattgcttc accgcctttg actaagatgc cccgtttcgc tccattaccg attcctgcta
     4861 cattcgaaac aggcgcacca atgactaaag cgccaggaca accaagaacc aaaacagtga
     4921 ttgctaaacg aaaatcacga ctaatcagac caacaaccaa cgcaataagt aaaacggttg
     4981 gagtataata ctgagaaaat cgatcaatga atttttccgc atgggatttg gtatcttgtg
     5041 cttcttctac taattcgata attttggcaa aggtcgtatc atctccgacc ttagttgctt
     5101 caatcgtcac gtagccatct gctaaaatcg ttccagagaa gacttcatct ccactgattt
     5161 tgacgacttg tcgtgattca ccagtcactg cggcttcatc cgcataccct ttcccgtcaa
     5221 tgactcgtcc gtccacagga atagctccac cagtttttac taagacgata tcgccttctt
     5281 ccacgtcatc gatttccact tcttcagtcg ttccatcttc ttggacaaca ctagcagtag
     5341 atggggccat ctcggtcaaa gccttgattg actcccgcgt tttggtcaat gttttttgtt
     5401 ccagatagct accaaacaaa aacaagaatg taacgatagc tgactccttg tattctccga
     5461 tcataaaggc accaagcaca gcaatagtaa cgagtaaatc aattgagata actttgactt
     5521 tcactgcctg atacgcatgg atcataatag gtatgaccgc aataatagaa gctatggcta
     5581 gagcaatgtt atagaacaag acaaaaccag taaattttcc gataaaacca aaaataatca
     5641 acacagctga taaggcggtg atatgatttc gatgctcttg gatccacttt tgtaatttca
     5701 tctttctccc tcttttcctt tcattcttac gagactaagt atagatactt atccatcata
     5761 aaaaattgac cactatcaag tttagtcaaa aaggcggttt tcttttggta gaatacacta
     5821 caaaaaatag ttagcaagaa gactcactaa aagtgatctt gccaactatt gtcgtttcta
     5881 tcgaaatttt cattgaatga ttttcctgta cagcatacat cggagatcta tttctttatc
     5941 taagattttt atcttgctaa ttttttcctt tagtctttca aatccacgtc gttcatagaa
     6001 ttgatacgta cagccactat ctgtaaaaag gaatatttct tttcctttct ctcttttttc
     6061 aaattctgtt agcaacttgg taccgatgcc ttttgttttc aaatcaggat ttgcagctaa
     6121 aaaaatgatc tctccatctg gcgaatgctt ctttaaatat gctgcaagca tttgttcatt
     6181 tgctgtctcg taagctcctt tactttcttt agcaaatata ttttgtaaaa aatcgaatat
     6241 cttaacatat atttgctgtc caaaagaacg atgtttcttt ttttctcctt ttatctccgc
     6301 cagcaaaaca cctgccaact cgtcctctgc atacaaagca agaatttgag ttgctcgatt
     6361 caactccata tacaaaaaat atcttccgta aagattcagc agaagttcat tttccatata
     6421 tctctcaaag ttcattcctt caacagcaaa atagatagct tttttgtaat ctttcttttc
     6481 taagggttta aaagttagtt tcatcatttc gcctcctcaa cttcaatgat tgtgttatac
     6541 ttacattata atagacacag tgtctactat ctataatcaa tcatctataa gtttgtcgat
     6601 taagcgacag ctttgctttg atatgtcgga taaggagata ggtaaatgaa aaagacagat
     6661 agacgcgtca aaaaaacaga aaaagctttg gctgaaacac tatccacatt attagtaaat
     6721 aaaaagattc aagcaatcac tatacgcgaa ctaacagaaa cggcagatgt acatcgttcg
     6781 actttttata cacattataa ggatatctat gatctatatg atcaattaga aagcaacttt
     6841 tttcgggatc taaatgaaat tctctcttat gatccagccc atagctatga ggaactatac
     6901 actcgcttga ttgactatgt ttacgcaaat acttttttat atcgcttatt tatcctcaac
     6961 catggtgata tttcgtttca gaagaaagtt aaacaagtta ttgaagaaaa ctatctgaac
     7021 atatggctat atgaagacaa gaaaacgatt atcacagaag aaatgagatt ctttactacg
     7081 taccacgtcc aaggttgtct atccgttctc aatcggtggg tagaaagcaa ttacaagtac
     7141 tccaaaaaag aattattaga actcttacag aaactgaatg atcacataga gaaaatcatg
     7201 ccttgaataa aaacatacta agcttagtta agaagaaccg taaccttctc gtataacaac
     7261 tgaatcttat ttagccaaat tgtttactga agattttttg agtaaaattt ctataattcc
     7321 tacttattga aaagtagttt gtagtgctgc ttctccctct ctaagtgata tatcttgttg
     7381 taccaatcaa attcacctca tcattaataa attactttta ggctaattta ttacaactta
     7441 taggttgaag ctgtaaccaa tttatagtca tattatattt ttatcatcca gtttattgaa
     7501 ttcttctcgt ttacctcaat tcattttcaa ggggaatca
//
LOCUS       DAYYCQ010000109         7432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000109 DAYYCQ010000000
VERSION     DAYYCQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.112"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1165
                     /locus_tag="ACHAYF_002616"
     CDS             158..1165
                     /locus_tag="ACHAYF_002616"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8579991.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1177..2181
                     /gene="neuB"
                     /locus_tag="ACHAYF_002617"
     CDS             1177..2181
                     /gene="neuB"
                     /locus_tag="ACHAYF_002617"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8579992.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2178..3326
                     /gene="neuC"
                     /locus_tag="ACHAYF_002618"
     CDS             2178..3326
                     /gene="neuC"
                     /locus_tag="ACHAYF_002618"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8579993.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3299..3976
                     /locus_tag="ACHAYF_002619"
     CDS             3299..3976
                     /locus_tag="ACHAYF_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8579994.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3927..5108
                     /locus_tag="ACHAYF_002620"
     CDS             3927..5108
                     /locus_tag="ACHAYF_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8579995.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5121..6098
                     /locus_tag="ACHAYF_002621"
     CDS             5121..6098
                     /locus_tag="ACHAYF_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8579996.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6098..7006
                     /locus_tag="ACHAYF_002622"
     CDS             6098..7006
                     /locus_tag="ACHAYF_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFM8579997.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            7007..>7432
                     /locus_tag="ACHAYF_002623"
     CDS             7007..>7432
                     /locus_tag="ACHAYF_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8579998.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSV"
BASE COUNT         2774 a          915 c         1400 g         2343 t
ORIGIN      
        1 ttttgtatca attttttctt tgatacttca tattttaatc aatcaaatgt tgattcgtta
       61 cgttttgaca tcattattga taattagttt agttatatat tttttaaaga ttaagaatcg
      121 aattatagat gactataatt cataggagga gatataagtg tttaaaggaa aaacaatatt
      181 agtaactggc ggaactggtt ccattggaag tgaaattgtt cgtcagcttt taaaatatga
      241 accaaaagct atcagaattt atagtagagg tgaagaaaaa caattttata tgcaacaaga
      301 gctctcaaat cacactaatt tgagattttt aattggtgat atcagagact ctcaaaggat
      361 tgattatgca tgtcgtgatg tggatattat ttttcatgct gccgcaatga aacacgttcc
      421 agcaagtgaa tataatccta tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt
      481 aatagatgca gcgatcaaaa ataatgtaaa acattttgta ggtatcagta cagataaagt
      541 agtgagtcct actaacacca tgggtgcgac aaagttattg agtgaaaaat taataatagc
      601 tgccaatcag tataaaggaa aaacagaaac aatattttct tgtgtaaggt ttggaaatgt
      661 catggggtca agtggatctg ttattcctct ttttagtaaa caaataaaaa acaataaaga
      721 tattactttg acagatgttg atatgacacg atttatgatg tcaataccgg atgctgcaag
      781 attagttctg tctgctgcta tgaattcaaa atttggagaa acatatgtct taaaaatgcc
      841 ggcaatcaat attttaactt tagctcaatc attaataaaa atatataatg atacacatga
      901 tgaaaaatat agaggtgaaa taaaagttgt aggtatcagg ccaggagaaa aattatatga
      961 agagctaatg actacagacg aaattgaacg ctcatatgaa aatgataaaa tgtatatcat
     1021 tccaccacac tatcatgcta ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac
     1081 tgcatactct tcagatgcaa cagaattaat gagtggacag gaatttatta gaatcgcaaa
     1141 agagaataat gtcgtgactt ggtaaggagt tttatgatga aatcagaagt atatgtaatt
     1201 gcagaaattg gtgtaaacca taatggaagt ctagatttag ctttagaaag tattgataaa
     1261 gctaaagaat gtggagctaa cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt
     1321 tccaaaaagg ctccaatggc aaaatatcag aaggataatc ttaaagagga cagttctcaa
     1381 tttgaaatgc taaaaaaatt agaattatct caatctgatt ttattagaat aaaagattat
     1441 tgtatacaaa ataaaattga ttttttgtct actccttttg atgaagaaag tgcagacttt
     1501 ctaaaacaaa ttggtgtaca aggatttaaa attggctcag gagatttaac aaatttacct
     1561 tttcttagaa aaatagatat gtatgggttg ccagttttat tatctacagg tatggctgat
     1621 attcatgagg tcagtgaggc tgtagattgt ttcatagata gtccagttac tattttacat
     1681 tgtacttcaa attatccggc tagttctgaa gatattaatt tattggcgat taacacatta
     1741 aaagaaatgt tcgggatacc cgttgggtac tccgatcatt cattgggata tgatgttggt
     1801 atttgtgctg ttgcgatggg tgctaaggta attgagaaac attttacctt ggataaggaa
     1861 ctacctggtc cagatcataa agcttcttta gatcctcagg aatttgcaga ttttgtaaag
     1921 catattagaa acactgaaat atttttaggt gatggtgtta agcgagcaat gccttctgaa
     1981 cttagtacaa aagaagtatc aagaaaaagc ctagtggtca ctgagaattt aaatgtggga
     2041 gatattctta cagagaaatc tcttacaata aaacgtcctg gaaatggaat agctcctaag
     2101 tttttatatc aatatattgg caaaaaagtt aaaagaccaa taagtgcaga tagtgttctg
     2161 cttgaggaag atgttgaatg aaaaaaatac ttatagtaag tggaacacga gctgattatg
     2221 gaatatataa acctgttgct gagaatttac tacaagataa ttgggatgta tcatttgttg
     2281 tttctggaat gcatttatcc cataaatatg gatttactaa agaattgatc ttagctgacg
     2341 gatttagaat tagtggagaa gttcaaagtt tgtttttaga gaacaataag ggaaacatgg
     2401 ctcggtctgt ggcattagag ataaatggtt tcacaaatat atttgaacaa actagccctg
     2461 attttgtatt attactaggg gatagaggag aaatgttggc agctgcgata gcttgtttgt
     2521 atcttggaat caaaacagca catattcacg gaggcgaagt gtctggtact gtagatgaat
     2581 caataagaca tgccataacg aaaatagcat ctgttcattt tgctgctacg gaaaaaagca
     2641 aagagcgttt aataaaaatg ggggaagatt cttggagagt ttttcccgtc ggagctccta
     2701 gaatagatac tattttaaat aatagattac ctagttttag agatataaaa gaaaaatatc
     2761 atctaaaatt tgaaaagaaa aaatacgttt tgcaagtatt tcatccagtt gtaaccgaac
     2821 tagaaaatat agaaaaacaa attttagctt tgatcaattc aatggaacaa caatctcaac
     2881 caattatttg cattttacct aatagtgatg ctggtagcga aattatcaga aaaatgtacc
     2941 agaaatattt aaaaacggat attatttata ttacgaattt agagccggag gaatacctga
     3001 ctatattaaa agagtgcaaa tttatgatcg gaaattcttc ttctgggata attgaagctg
     3061 catcattcaa aataccagta ataaatttag gaaccagaca aaatggacga gaacaatcta
     3121 aaaatattat aaatgcaaat ctaaatgaaa atgaaattga aactgctttg aataaaatac
     3181 aagatcctca gtttataaaa agcttgaaaa atgttaagaa catctacggt gacggcaaca
     3241 gttctagaag aataacaaaa acattgaatc aaatattgaa tgatggggaa gatattaagt
     3301 ggattcaaaa acaaatagct tattaaaata tactagtgat ccatacatta ttattggggc
     3361 tggcggacac gcaaaagtca ttattgaaac cttaagatta gaaaataagt taatatatgg
     3421 attttttgat gataagcaaa ataaaatgca agatctgaaa tatcttggga aaataaacga
     3481 attgaatagc tgggcggata gcaaaaaaaa aatctataaa tttattgtag ctattggtga
     3541 ctgtgtagta agaaaaaaga ttataaaaaa attgaatatg ccaagtgagc aatatggaat
     3601 agctgttcat ccaagtgcta tagtggcaaa agaaatttca attggttatg gaactgttat
     3661 ttttgcgaat gctgtaatta acccagatgc tgtaattgga gagcatgcta taataaacac
     3721 aggtagcatt gttgagcatg attgtagaat taataactat gttcatttat ctccaggtgt
     3781 atgcttagcg ggtggagtac atgtaggtgt aggaactcaa ataggaattg gttctcaatg
     3841 tatccagatg aagaaaattg gatcttggtg tatgataggc gcagggagca ctattgttaa
     3901 ggatattccc tcacattcct tagcttatgg caatccagct aaaattaaga aagaaggaat
     3961 taattttgaa ttctagaatt ttattatcaa gtcctcatat gggaggaaca gaagaaaaat
     4021 atgttgctaa ggcttttaaa gataattgga tagcgccatt aggtgaaaat gtggataaat
     4081 ttgaagaaag tattacaaaa tatactggtt ctaagggcgg agcttctgcg ttaagttcag
     4141 gtacagctgc catacatctt gccttaagac tgcttaatgt tggaaaaaac gatattgtct
     4201 ttgctccttc atttactttt atagctacag taaatcctat tttatatcaa ggggctgtac
     4261 cagtgttcat tgactcagaa cctaatacat ggaatatgag ccctgttgca ctagagagcg
     4321 cattagaaga agctgaaaga aacaaccaat tacctaaggc aatcatcgta gtgaatttat
     4381 atggtcaaat ggctgattat gaacgaattt tagctattgc aaaccaattt aatgttccag
     4441 tgatagaaga tgcagcggaa tcgttaggtt ctacttataa gggaatttcg tcaggcttat
     4501 ttgggacaat tgggatctat tcatttaacg gtaataagat catcacaacc tcaggtggag
     4561 gagctctagt gtctagtgat aaaaaatatg ttgagcattc aagattttta gcaacacaag
     4621 caagagatca agcaccgtat tatcaacata gcgaaatagg ttataattac cgtctaagca
     4681 atatttctgc cggtatcgga cgtggacaaa tggaagtatt gaatgataga gtagctaaac
     4741 gaagagaaat tttcaatcga tataaggaag ctttttctgg aattgataga attgaaatga
     4801 tgccagaagt ggatggcatg gtgccaaatc gttggttgtc tactttaacg atcaatgatt
     4861 ataattctat tgatcaagta gttaacgtaa taaataaact aaatgaaaaa aacattgaaa
     4921 caagagcttt gtggaaacca ctacatttgc aaccagtatt tcaaggggta aaaatgtatt
     4981 accatgataa taatcttagt gtgtcagaaa agttatttaa tagcggctta tgcttacctt
     5041 ctggaagtaa tatgacagat agtgaacaag aatttgttat tgatgagttt aaaaaaatat
     5101 tcaagtaggt gtgatatttt atgaacatag gaattattgg tttaggttcc atggggaaaa
     5161 gaagacttag actcattcaa gaacacttta atgatatttt actatgtggt gttgatacta
     5221 atgaggcgag acgaaaggaa gttgaaaagg aatttggaat accaacttat aatagtctaa
     5281 cagaggcaaa aaatttgttt gaaataaatt cagtttttgt ttgtacatcc cctataaccc
     5341 atgaatttat tataaaagag gctttaaatt tagattgtaa tatctttacg gaaatcaatt
     5401 tattaaatga atattatgat gaaataatcg aagtagctaa aaagaaagat agattacttt
     5461 atctatcttc tacatttttg aagagaaaag aaattcaata tattaagtct aaagtgacgc
     5521 ataataaaaa aatttcttat caataccatg taggacagta tttacccgac tggcatcctt
     5581 gggaagatta tcgcaatttt tttgtttcaa acaaaaaaac taatgggtgt agagaaatat
     5641 tcgctattga attaccttgg attatagata catttgggaa agttaaatcg tttactgtaa
     5701 tgacccagaa attatccaat cttgatattg attattcgga cagtttcact cttattattg
     5761 agcatgaaac tggtatagtt ggaactttaa gtgtagatgt tgtttcaaga gtagctaaaa
     5821 gaaatttaac tataattggt gaaaagattt taatagaatg ggaaggtaac ccggattctc
     5881 tattttcatg gaatgaagaa acttctagta tgacagatat tcaattgtac gatgaggtta
     5941 tgcatgataa taattattca catactatta ttgaagatgc atatttagag gagattaaag
     6001 aatttttcac gcttttggaa aagaaaatgg aaccccaata tacttttgaa aaagataagt
     6061 atattattaa cttaattaac gacattgagg taaattaatg atgaaagtaa ttttttttgg
     6121 gttagggtca attggaaaga gacatctaaa aaatctaata agctatggaa aaaagaattc
     6181 tattaatttt gatataacag cttatagtag cagtggaaaa aaagagtttg aagaaataac
     6241 gtatattcaa aatagagacg atttggatac acattatgat atagctttta ttacaaatcc
     6301 gacttttaag catctggaaa cactaaatgt aattgttgaa aaagttgatt acatattttt
     6361 agaaaaacct gctttttctg agagtattga tcttactcaa tataattatg attacaaaaa
     6421 tatttatgtt gctgctccac tgaggtataa aaatatcatg gaagttgttc gtaatagtat
     6481 tcaaagtaaa aaagtatatt ctgtaagagc tatttgttct acttatcttc ctgattggag
     6541 aaaaggtact gattatagaa ataattattc tgcctttaaa tctatggggg gaggagtaga
     6601 attagactgt atacatgaat tggattatat tataaaccta tttggtttcc ctaaatcagt
     6661 aaaaaaacag tttggtaaat attcgaatct tgaaattaac agtaatgatt tagcaacata
     6721 tatattgaga tacgatgata aaattataga aattcattta gattattttg gaagagttcc
     6781 tacaagaaaa tttcaattgt ttactttaga tgaatgtatc aatgttgact tattaaataa
     6841 taaaataagt tttgaagaaa acattgttaa agaaatgcct gaatctgcta atgatatgta
     6901 tgagaaggag ttgaaatatt tctttgaaaa cgtagtaaca ggcaaagaaa actggaatga
     6961 tttatatcat gcaaacgaag tattgaaaat agctgaggag aaataaatgg aaattttatt
     7021 tactatttgt ggtagagctg ggtctaaggg gtttaaaaat aaaaatttga aatatttggc
     7081 aggaaagccc ctagtccatt tcgctttagc aatgatagat atctatcaac aaaaacatcc
     7141 tgaacataat tgcacgactt gtttgaatac ggacagtaat gagttagtag aattgacgac
     7201 taagttaaat ccgtctgtga aaattgtaga acgtaaacct gaattagcta cagatactgt
     7261 agggaaaatt gaagtgatac gtgattcatt cttacaacta gaaagagaag ataatatatt
     7321 tgatttaatt atagatttag atttgacttc acctttacgt gaagtttcta atcttgaaga
     7381 attaatagat gaacatctaa aaaatttaga taaagacgtc tacttttcag tt
//
LOCUS       DAYYCQ010000110         7389 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000110 DAYYCQ010000000
VERSION     DAYYCQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.113"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACHAYF_002624"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACHAYF_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8579999.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACHAYF_002625"
     CDS             complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACHAYF_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8580000.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACHAYF_002626"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACHAYF_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8580001.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="ACHAYF_002627"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="ACHAYF_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8580002.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACHAYF_002628"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACHAYF_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8580003.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1975 a         1638 c         1289 g         2487 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaact
//
LOCUS       DAYYCQ010000111         7242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000111 DAYYCQ010000000
VERSION     DAYYCQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.114"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..575
                     /locus_tag="ACHAYF_002629"
     CDS             <1..575
                     /locus_tag="ACHAYF_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8580004.1"
                     /translation="LLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEP
                     KYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLI
                     PLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAA
                     AGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            587..1507
                     /locus_tag="ACHAYF_002630"
     CDS             587..1507
                     /locus_tag="ACHAYF_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8580005.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1548..3005
                     /locus_tag="ACHAYF_002631"
     CDS             1548..3005
                     /locus_tag="ACHAYF_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8580006.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3350..3964
                     /locus_tag="ACHAYF_002632"
     CDS             3350..3964
                     /locus_tag="ACHAYF_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8580007.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3957..5681
                     /locus_tag="ACHAYF_002633"
     CDS             3957..5681
                     /locus_tag="ACHAYF_002633"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8580008.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5678..7129
                     /locus_tag="ACHAYF_002634"
     CDS             5678..7129
                     /locus_tag="ACHAYF_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8580009.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2476 a         1206 c         1448 g         2112 t
ORIGIN      
        1 ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa
       61 gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt
      121 ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga
      181 ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg
      241 aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac
      301 aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg
      361 attttggcct gttttataac attcctagga attccggtgc attatatgat gtgactcaag
      421 ttttggatac gtatatctac aatggcctga catccacagg agattttggt atgacggctg
      481 ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg
      541 cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa
      601 agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat
      661 gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt
      721 taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat
      781 atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt
      841 cactgtactt gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc
      901 ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttgat
      961 tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca
     1021 gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat
     1081 tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat
     1141 cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg
     1201 tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt
     1261 actatacatc caagatgata agctagttcc actacagtat cttttgatga aaatccaaag
     1321 taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc
     1381 ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc
     1441 gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa
     1501 agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga
     1561 agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag
     1621 gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa
     1681 tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa
     1741 aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa
     1801 tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt
     1861 ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa
     1921 aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat
     1981 atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt
     2041 tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag
     2101 tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct
     2161 tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc
     2221 tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt
     2281 tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca
     2341 ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt
     2401 atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa
     2461 ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata
     2521 cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat
     2581 tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag
     2641 atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata
     2701 caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg attgccaaac
     2761 gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg
     2821 acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat
     2881 tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca
     2941 aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag
     3001 aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc
     3061 gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg
     3121 gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact
     3181 cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac gacaaaatat
     3241 gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc
     3301 tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa
     3361 ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg
     3421 gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa
     3481 tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa
     3541 tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac
     3601 tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc
     3661 tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc aatacagtat
     3721 gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa gttttatttc
     3781 ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta taattttagg
     3841 cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt tgacggtttg
     3901 gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg aaagttatga
     3961 ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg
     4021 cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc catacttggg
     4081 aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc acaagcagaa
     4141 tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta gggaattccg
     4201 aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat ctgatgtatg
     4261 tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa atcaaagatc
     4321 tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt
     4381 attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca ccaaaattac
     4441 tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca ggaagtattt
     4501 ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg tcattcaatg
     4561 gactctcttt atacatgatt tctggaacga caaatcgact atacacattc catgataata
     4621 aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt ctgaagaaaa
     4681 attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac caagaactgt
     4741 ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa
     4801 aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg cttatctttc
     4861 tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag gccatggatc
     4921 aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa tatgaactga
     4981 aggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt gtagaagaca
     5041 tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa gcacagatca
     5101 atccacactt tttatataat acattagaat atatccgaat gtatgcaatc agcgaaggaa
     5161 gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc
     5221 aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat gtctatttgt
     5281 atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct gatttggaaa
     5341 agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt aaacacggta
     5401 ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg
     5461 taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta aaacagattg
     5521 aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg caaaacgtga
     5581 atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag aataatgaga
     5641 caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc aagtgttatt
     5701 ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt
     5761 aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg tacttgaaca
     5821 gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg gactggaatt
     5881 tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat caggatacca
     5941 agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt tgatgaaacc
     6001 cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt tagatcagca
     6061 aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc aatggttgaa
     6121 tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg agaagcagca
     6181 taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata gatggttgca
     6241 agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat tgatcacgtt
     6301 gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa actttcctga
     6361 tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa tccctgaaac
     6421 attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata aagacgagca
     6481 tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt caagtttcag
     6541 acgcgtgctg caaaataaac aattggaagt cgctcaaaaa atgatcactg atttttttga
     6601 acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt ttttactgtt
     6661 tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac aaggaatcga
     6721 aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt cattcatcaa
     6781 aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg taatcctgca
     6841 ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc atttaaatgt
     6901 catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa
     6961 ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa acatcaatga
     7021 gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat tcaagaaaat
     7081 cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa aaaaaaagcc
     7141 aatgtaatga aataacttat aaaaaattgt ctagaaatct aattttgggc tgttcttatt
     7201 acttgaggca aggcagcttt ttcatgacct ctcgataaac gg
//
LOCUS       DAYYCQ010000112         7153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000112 DAYYCQ010000000
VERSION     DAYYCQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.115"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1368)
                     /locus_tag="ACHAYF_002635"
                     /pseudo
     CDS             complement(1..1368)
                     /locus_tag="ACHAYF_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="ACHAYF_002636"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="ACHAYF_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8580010.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2331..2429
                     /locus_tag="ACHAYF_002637"
                     /pseudo
     CDS             2331..2429
                     /locus_tag="ACHAYF_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2652..2882)
                     /locus_tag="ACHAYF_002638"
     CDS             complement(2652..2882)
                     /locus_tag="ACHAYF_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8580011.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3112..3930)
                     /locus_tag="ACHAYF_002639"
     CDS             complement(3112..3930)
                     /locus_tag="ACHAYF_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8580012.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4091..4780
                     /locus_tag="ACHAYF_002640"
     CDS             4091..4780
                     /locus_tag="ACHAYF_002640"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8580013.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4794..6296
                     /locus_tag="ACHAYF_002641"
     CDS             4794..6296
                     /locus_tag="ACHAYF_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8580014.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6309..6785
                     /locus_tag="ACHAYF_002642"
     CDS             6309..6785
                     /locus_tag="ACHAYF_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8580015.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6882..6992)
                     /locus_tag="ACHAYF_002643"
                     /pseudo
     CDS             complement(6882..6992)
                     /locus_tag="ACHAYF_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2316 a         1131 c         1295 g         2411 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgatga tctgtctaac ttaatttgac aattaagagt atatttactt cagatggtaa
     2461 ggcaacaaaa taaaatacaa taggcagatg aactaataaa taaaatctgt attaaataca
     2521 gataaattaa tataaaaatc aaaaggatca taaactttta acaagtgatg aaatgggtgg
     2581 aaattgattc ttatagacag tttgtctcat aacattgaag tattttgcaa ttttagcatt
     2641 gaaacacttt tttaatcttc aacgttgttc ttataaacaa gatgattggg aatcttttga
     2701 attaacactg tatagcttag aacacccttt atagaaaaca ttcgcttagc aatcttaata
     2761 attccttgtg cccgatgtcg tttagattca gtaggttcct gctcatcgat catggaaaaa
     2821 atttgaagga tcagatcctt aataaattta tttaagagag gatgatccat tgcttcatcc
     2881 atactcgata aactagtgat aatgagctac acgtttttct ttcaagttga tcaatagctt
     2941 ccacgataaa aatatcttta gataaaatca agaaccgtag gaactacagg gatatttggt
     3001 caataagagc caccgctatt agtaaaaaat aagctatcaa gtatgctctc atcccaagaa
     3061 ttgcctactt caatcgttaa aaaccgttag gtttagctaa gtgggggtag ttcactcaat
     3121 acttttatca ataatagagc gcaaaacttt ttctcttaat tcaactgaat caatattatt
     3181 gttttgttca taaccatgaa ctaatagatc acaaatataa agttgtgata tttttccagc
     3241 taaagatcca ccattaagaa attcttcaat ggctgtttgt aaaactaagt ctgcagactt
     3301 tcctataggt gaatgaatat agttagttat agcaataatt tttgcgccat tttttttagc
     3361 tattttcaat gaatcataag tatctttcgt tttcccagat aaagaaaaaa tgattactaa
     3421 atcattaact gttaataaag aagcgacttg ggcttgaaaa tgagggtcta gtacagcttt
     3481 tgcttgaact cctacgcgaa gaaacatatt ttctaaatca agactagtat ttccactaga
     3541 tcccactcca aatatatata tgttttttga ttgagtgatc attttaatgg cttcattcaa
     3601 tttttcttcg tctaaaagcc taatagtgga atagagagct tctgttaaac tattagcaat
     3661 ttcatcatag tactttacac tagtcggatg ttcttttttt tgactaaagt cttcctttgc
     3721 aatttcaatt tttaaatcag aaaaaccaga aaaaccaatt ttttgacaaa agcgaataat
     3781 agtagcatcg ccaacttttg tgtttttctt aatatcactc atcgtactat aaataatagt
     3841 tttcccagaa tttaaaataa aatcagcgac ttttttttca gacttcgtta aaaatggata
     3901 attttcttta attaaataga tataactcat acaaactcct ctacaatttc aacttttctc
     3961 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
     4021 tgaaagcgga tgctatattg tttttggagt tgttatccaa aaataataaa aaattggagt
     4081 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
     4141 ctgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
     4201 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
     4261 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
     4321 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
     4381 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
     4441 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
     4501 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
     4561 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
     4621 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
     4681 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
     4741 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
     4801 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
     4861 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
     4921 ttttagatca accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     4981 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     5041 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     5101 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     5161 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     5221 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     5281 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     5341 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     5401 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     5461 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     5521 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     5581 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     5641 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     5701 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     5761 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     5821 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     5881 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     5941 tatatttctt tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg
     6001 aagaagaatc agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag
     6061 agtctcttat cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt
     6121 gtgcaactcg tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa
     6181 aagacactgg tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag
     6241 ggaaagctga cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg
     6301 tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt
     6361 ccggaaaaat catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag
     6421 gtgatggttt tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag
     6481 taacaaatat atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt
     6541 atttgattca tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg
     6601 ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa
     6661 taataaaatc tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca
     6721 atgatttaat aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt
     6781 tttaaaaaag tgtatataaa aagctataaa ataagctaaa taggatattt gtcaaatgaa
     6841 gttggtgaac agaaagaact gttttctttc tgttcatttt tttattttat tagtaatgat
     6901 tccccatgcc aaatcatgat ccgtaattta aagttataaa agcttcgaaa tccattggca
     6961 tttcgttgga ataccttgat atgattattc aaatataaaa tatagagaga agagagagag
     7021 ggaagatatt tataaagggc aaagttatgt ttactcaact taggaaatag aaatagatca
     7081 atttatattc tcttgtcaat cgaatatgaa ccttagcact atataccaaa tatagtaaaa
     7141 tgctgaaaga tgt
//
LOCUS       DAYYCQ010000113         6626 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000113 DAYYCQ010000000
VERSION     DAYYCQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.116"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..493)
                     /locus_tag="ACHAYF_002644"
     CDS             complement(35..493)
                     /locus_tag="ACHAYF_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8580016.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(506..1837)
                     /locus_tag="ACHAYF_002645"
     CDS             complement(506..1837)
                     /locus_tag="ACHAYF_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8580017.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1848..3290)
                     /locus_tag="ACHAYF_002646"
     CDS             complement(1848..3290)
                     /locus_tag="ACHAYF_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8580018.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3277..4041)
                     /locus_tag="ACHAYF_002647"
     CDS             complement(3277..4041)
                     /locus_tag="ACHAYF_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8580019.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4145..5020)
                     /locus_tag="ACHAYF_002648"
     CDS             complement(4145..5020)
                     /locus_tag="ACHAYF_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8580020.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5275..5359)
                     /locus_tag="ACHAYF_002649"
     tRNA            complement(5275..5359)
                     /locus_tag="ACHAYF_002649"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5323..5325),aa:Leu,seq:caa)
     gene            complement(5377..5448)
                     /locus_tag="ACHAYF_002650"
     tRNA            complement(5377..5448)
                     /locus_tag="ACHAYF_002650"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5414..5416),aa:Gln,seq:ttg)
     gene            complement(5458..5530)
                     /locus_tag="ACHAYF_002651"
     tRNA            complement(5458..5530)
                     /locus_tag="ACHAYF_002651"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5495..5497),aa:His,seq:gtg)
     gene            complement(5592..5676)
                     /locus_tag="ACHAYF_002652"
     tRNA            complement(5592..5676)
                     /locus_tag="ACHAYF_002652"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5640..5642),aa:Leu,seq:caa)
     gene            complement(5697..5767)
                     /locus_tag="ACHAYF_002653"
     tRNA            complement(5697..5767)
                     /locus_tag="ACHAYF_002653"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5733..5735),aa:Cys,seq:gca)
     gene            complement(5813..5884)
                     /locus_tag="ACHAYF_002654"
     tRNA            complement(5813..5884)
                     /locus_tag="ACHAYF_002654"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5850..5852),aa:Gln,seq:ttg)
     gene            complement(5896..5968)
                     /locus_tag="ACHAYF_002655"
     tRNA            complement(5896..5968)
                     /locus_tag="ACHAYF_002655"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5933..5935),aa:His,seq:gtg)
     gene            complement(5971..6044)
                     /locus_tag="ACHAYF_002656"
     tRNA            complement(5971..6044)
                     /locus_tag="ACHAYF_002656"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6010..6012),aa:Trp,seq:cca)
     gene            complement(6051..6131)
                     /locus_tag="ACHAYF_002657"
     tRNA            complement(6051..6131)
                     /locus_tag="ACHAYF_002657"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6095..6097),aa:Tyr,seq:gta)
     gene            complement(6133..6208)
                     /locus_tag="ACHAYF_002658"
     tRNA            complement(6133..6208)
                     /locus_tag="ACHAYF_002658"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6173..6175),aa:Phe,seq:gaa)
     gene            complement(6244..6316)
                     /locus_tag="ACHAYF_002659"
     tRNA            complement(6244..6316)
                     /locus_tag="ACHAYF_002659"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6281..6283),aa:Asp,seq:gtc)
     gene            complement(6326..6398)
                     /locus_tag="ACHAYF_002660"
     tRNA            complement(6326..6398)
                     /locus_tag="ACHAYF_002660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6363..6365),aa:Val,seq:tac)
     gene            complement(6414..6485)
                     /locus_tag="ACHAYF_002661"
     tRNA            complement(6414..6485)
                     /locus_tag="ACHAYF_002661"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6450..6452),aa:Glu,seq:ttc)
     gene            complement(6500..6589)
                     /locus_tag="ACHAYF_002662"
     tRNA            complement(6500..6589)
                     /locus_tag="ACHAYF_002662"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6553..6555),aa:Ser,seq:gga)
BASE COUNT         1986 a         1465 c         1083 g         2092 t
ORIGIN      
        1 aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa
       61 tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga gaaactacaa
      121 tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata gctaacagtg
      181 tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt ccttcgagtt
      241 caactgtatc taaacccata tggatcagta tttttctgcc agattcactt tcgatcgtta
      301 tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca tggatatatc
      361 catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag acctcgtctt
      421 ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg tttcttgttt
      481 taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt ttgtttactt
      541 tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt tgattcaatg
      601 taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact agtaacttct
      661 ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt tttcttagtg
      721 taattggctc cacaccagtt gcaccaacat atgccggtat ttcaacaact gcatctgacc
      781 gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga cgatcatcat
      841 tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa ttaaagttca
      901 atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta tttttctctc
      961 gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca acgaccatat
     1021 caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa tttttcgtga
     1081 tgactgacat catttgaaag gttttttgcc aagttttatc tcctatatta aaatcggcga
     1141 cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct cgttgcaatt
     1201 ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac gtaacgatcc
     1261 aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa atagtcatat
     1321 cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca gaaacgatcg
     1381 actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt atatgatcga
     1441 caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg agtccacacg
     1501 tttcttgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct cgcatctcca
     1561 atcctccaac acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca agctttggat
     1621 catccgttgc acgaatgcga atatttgaaa aaccttttct tttcagcata aatttaacaa
     1681 tcaagtacat atccatattt ctttcttcat caatatcata taaacgtatt tcagataatg
     1741 gcaaccgttt ttcattgttc aaaatggctt gaacgattcc tggtgtatac gtactcccgc
     1801 ctccagctat agtgataatc tgttttttca tcatcatctt ttcctttcta ttcttctaat
     1861 aatttattga attctgccat aacataagtc acttctaagc cgatgataat ttgataattt
     1921 ttttcagttg gtttaatgat cccgcttatc cctaattgct tgattcgctg ttcctcgacc
     1981 atggtgggat ccactacctc tacccgtagg cgtgtattac aactatagac atccaggata
     2041 ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca tatattcata atttttactt
     2101 gctaacccta actctgtttc ttctaaggtt acttcattgc caaactctgc cttagcttca
     2161 cgtcctaatg tctgaatgtt tctcttattg atcaaataat aaaaagtaac gtaatacaag
     2221 gcaaaaaaga aaattccgat tggaagaatc aaaatagcat tggtagcgat tcgaaaattg
     2281 atgagataat caacaataca tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa
     2341 cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat aggagaaaga
     2401 aacatgaaac taaattcaac aggctcagtg atatttgcaa taaatgaagt tcctgcaccg
     2461 cttcccatga ttcctttgac catctcttta ttttctttat aagctgcttt atagatagct
     2521 agacatatag ctggtatacc aaacatcatt actacaaaaa aaactgataa aaacagtcca
     2581 gcctgaggat ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc cttgctagga
     2641 gatgtatagc ttcctaaatc gtaataaata tacgtattca acacatggtg cagtccaaag
     2701 gggatcagca aacgattcaa aaacataaat atccccacac ctaatgctcc catatcaacg
     2761 agcagttttg caaaattatc aatcccaatt tggatgagcg gccagataaa gcccaaaaaa
     2821 acagctgcga ttgtttgtag aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa
     2881 taagaaaaaa ctactggtaa tttttgttct ttaaagcgat tatacgtcca tgccgctact
     2941 aaaccggaaa taattccacc aaaaatcccc atattcactt ctgggtccat aatttctaag
     3001 ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta
     3061 tttttgacaa aaccgattgc aactcctata gcaaataaga tatccaagtt tccaaaaacg
     3121 gcatttccag ctgcaccaat tactgggatg ttcagcatat cgtcagctga taaacgcatg
     3181 atcaatcccg ctaatggcat cgctgcaact ggaagtagca tcgctcttcc aagcttctgc
     3241 acatttgatt taacattcat ttttattcct cctctattaa atatcctcat atgttactaa
     3301 ctcaatcaaa ggatcatttt tcactttttg agcaaatgct gaatgaatta aaaattcgac
     3361 ttggtgggta cgaggataca caaaacttga accatttaac aattcctgat ccaaatacgc
     3421 tgcgtgacac atgacttcga tcgttttatc ttctctcaat atcatgagta aacttgccaa
     3481 atattcttcc acactcgtat tgatttgtcg ttttttttca tcagtttctc ctacgacatc
     3541 gaaacaaggc tcaaaataat cgacatgacg aacttcatct tttcgttcaa agttgctccg
     3601 aaccggcaaa tcatattttt ttgctaatgt aacaactacc tcctgatttt catccaaagt
     3661 atgagtatga tgatgactgt ctaaatgagt tggtatgatc cccgcatgat aaattttttg
     3721 aatctgagca ttccattcct gatacagttg atctttgtca atgttaaatg gatgctgata
     3781 ataagataag gatctgaact ctccgctttc gataagagta tcgacagttt tgaggagtgg
     3841 tttgccacac gtcaatgtta aatggactcc tacacctagc ctaggcattt cctttcttag
     3901 tttcacagca tgctcgaaac ccggcatatt tgccatcaat gtagttgatg tcaaaattcc
     3961 tcgctgataa gcatccataa tcccataatt cacaccatgg ctatagccaa aatcatccga
     4021 attaaaaatc acttttccca tttcattctc cttctttttt ctactaaaat aggcgcaacc
     4081 aaataatcat agtccattta ctatgtgtca tttggttgcg cctatagata gttacgccct
     4141 tattttaata gattcttgct agatgaattg ccaaatagat gatttcattt tcttgaatgg
     4201 caagttgaaa ttgctgttcg atgatatctt taatctttag tgctgtacaa tacgctttgc
     4261 tatgtttaaa tgtttcagta aaaaaaccaa tattttctac tggcgtttct tcctgataaa
     4321 tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg atgataagtc agtgattctt
     4381 tatcataaac gtttgaatag gatttttcta gatgggacaa aatcgtggtc gtcatttcta
     4441 ctatttttaa gctctttaaa ctattaggct ctcctagctc agcattaatg atatgcatag
     4501 tgatgaatcc tacttcattt gaccctaact cggtttgata tcgatgattg attcgctcga
     4561 ccacttctac tgctatatca tattcatttg gataaaggaa tctcatttct ggcaacataa
     4621 ttgctgtgac atctctatct tctctttttc gttgaatagc aaaataaata tgatctgtca
     4681 atgtaatata gatatttgaa tctaatggag aggagatcct agctgttatc agttcaatca
     4741 cttcttccgc aaaatccata aattcgattg gaatctgcga gactatctca ctgaatcgtt
     4801 tgatcttttc tttagaatct aatacaaata atttgtcagc atcctcaaca tcaaatggct
     4861 tactcacttt agcagaaaag ccaatccctt tccccatcgc aattttttct attccatcgt
     4921 catcgatcaa tacagcatta tgattcagct ttttcatcac atacaccgtt aattcacctc
     4981 ctctatatga taaaacaaaa caacttgcag actccttcat acatattttt gtctccaaac
     5041 aatttttaaa gacaaaaaac gacctaagaa agaacccttt cttaggtcgt gcctaattga
     5101 atagtagcac cctacataat ttacaaaaat agtttaacac ataataaaag cgttatcaat
     5161 atctttttta ctcgttttat gataatactt gtatcgaatt gatttggagg taattacata
     5221 aaaacaaaaa aaatatagca actctctata agaaagttgc tatattgata attataccgg
     5281 cggccggggt cgaaccggca cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg
     5341 ccaattccgc cacgccggca aatttaaaaa taataactgg ggtagctgga ttcgaaccaa
     5401 cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaagggc
     5461 gaatgatggg aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt aaccacttcg
     5521 ccacattcgc catcataaaa gatgttattt aatttctcaa taaaaaacta agatgaagac
     5581 atcttagtca ataccggcgg ccggggtcga accggcacgc cctcaacggg cactggattt
     5641 tgagtccagc gcgtctgcca attccgccac gccggcaagt tgatttatta gtactaaggc
     5701 ggtaaccgga tttgaaccgg tgatgaaggt tttgcagacc tctgccttac cacttggcta
     5761 taccgccatt atactattat caatctataa aataaagaat aaattataga aactggggta
     5821 gctggattcg aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac
     5881 cccaagaata aaaagggcga atgatgggaa tcgaacccac gaatgccgga gccacaatcc
     5941 ggtgcgttaa ccacttcgcc acattcgcca tggcaggggc agcaggaatt gaacccacac
     6001 taacggtttt ggagaccgct gttctacctt taaactatgc ccctataaaa tggaggagag
     6061 tggattcgaa ccactgaacc ctaaggaacg gatttacagt ccgtcgcgtt tagccacttc
     6121 gctactcctc catggtggcg cgggacagaa tcgaactgcc gacacacgga gcttcaatcc
     6181 gttgctctac caactgagct accgcgccat attttaaaat tattaattga ataacaagaa
     6241 cgacggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg catgttaacc
     6301 cctacaccac ggaaccagtt cattatggag gttaacggga tcgaaccgct gaccctctgc
     6361 ttgtaaggca gatgctctcc cagctgagct aaacctccaa cattcaatta aaatgacccg
     6421 tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg cttgaccaac
     6481 gggccaggta aatttgctac ggagagtaag ggattcgaac ccttgagaca gtttggcacc
     6541 atctacatga tttccaatca tgctccttcg gcctctcgga caactctcca gagtgaaaga
     6601 agctcaatac tctttctcaa aatgag
//
LOCUS       DAYYCQ010000114         6615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000114 DAYYCQ010000000
VERSION     DAYYCQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.117"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHAYF_002663"
     CDS             complement(1273..1938)
                     /locus_tag="ACHAYF_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8580021.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACHAYF_002664"
     CDS             2064..4100
                     /locus_tag="ACHAYF_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8580022.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHAYF_002665"
     CDS             4104..4742
                     /locus_tag="ACHAYF_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8580023.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHAYF_002666"
     CDS             4770..5198
                     /locus_tag="ACHAYF_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580024.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACHAYF_002667"
     CDS             5655..6566
                     /locus_tag="ACHAYF_002667"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8580025.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2276 a         1001 c         1220 g         2118 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaattattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcat
//
LOCUS       DAYYCQ010000115         6326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000115 DAYYCQ010000000
VERSION     DAYYCQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.118"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1957)
                     /locus_tag="ACHAYF_002668"
     CDS             complement(26..1957)
                     /locus_tag="ACHAYF_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8580026.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="ACHAYF_002669"
     CDS             complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="ACHAYF_002669"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8580027.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2886..3638)
                     /locus_tag="ACHAYF_002670"
     CDS             complement(2886..3638)
                     /locus_tag="ACHAYF_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8580028.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3797..4006)
                     /locus_tag="ACHAYF_002671"
     CDS             complement(3797..4006)
                     /locus_tag="ACHAYF_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580029.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4017..5344)
                     /locus_tag="ACHAYF_002672"
                     /pseudo
     CDS             complement(4017..5344)
                     /locus_tag="ACHAYF_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5480..5635
                     /locus_tag="ACHAYF_002673"
     CDS             5480..5635
                     /locus_tag="ACHAYF_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8580030.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5709..6296)
                     /gene="yihA"
                     /locus_tag="ACHAYF_002674"
     CDS             complement(5709..6296)
                     /gene="yihA"
                     /locus_tag="ACHAYF_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8580031.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         1821 a         1339 c         1099 g         2067 t
ORIGIN      
        1 aaacccagcc tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata
       61 aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg
      121 ggtgactcag taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga
      181 aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa
      241 tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga
      301 tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag
      361 caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac
      421 ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt
      481 tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag
      541 cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag
      601 ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga
      661 ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata
      721 cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg
      781 tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg
      841 aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt
      901 tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc
      961 caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag
     1021 gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat
     1081 ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac
     1141 cactaagggc tagattgatc aactcttctg gtttacggat accatcactg acaggacgat
     1201 tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta
     1261 caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt
     1321 tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg
     1381 ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt
     1441 ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt
     1501 tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta
     1561 attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat
     1621 gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt
     1681 ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa
     1741 ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg
     1801 cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa
     1861 acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga
     1921 tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc
     1981 aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc
     2041 atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc
     2101 tttcgtacgg ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga
     2161 atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa
     2221 agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt
     2281 cccatacgga atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc
     2341 gtgattgttt ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc
     2401 tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata
     2461 aaatcctttt cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc
     2521 tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa
     2581 gccattgatc tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt
     2641 tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc
     2701 agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg
     2761 ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc
     2821 gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt
     2881 gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct
     2941 aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac
     3001 aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct
     3061 aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca
     3121 ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta
     3181 gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc
     3241 tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt
     3301 ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct
     3361 tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg
     3421 agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca
     3481 tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat
     3541 tcagaagcat tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc
     3601 aagtctagga ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa
     3661 ataatagcat attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt
     3721 ataaatcagt caaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt
     3781 ctatcatcaa gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag
     3841 aagcaggttt tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga
     3901 ccaaaaaacc gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata
     3961 ataatgatgc cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat
     4021 attttttgta aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga
     4081 tgctgctcac ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg
     4141 aaggtaacat cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct
     4201 gaaggaacat agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct
     4261 acttctagat attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca
     4321 gtcggatcga taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa
     4381 tacgcctgag catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata
     4441 tcataacttt ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca
     4501 ttctgatact ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt
     4561 ttcaataaac cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa
     4621 taggattgaa acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg
     4681 atacatatga cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa
     4741 atcggtttag ggatgatttg atgataatag cgactataat acatttccca acacgaagtg
     4801 aaacataaac cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact
     4861 tccccgtttt gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat
     4921 agccgttgag agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag
     4981 gcgaacaccc catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt
     5041 tctttgaaaa cagagataga caatacattt tttatcaata attgattatc ttccaagcga
     5101 aaactacatt cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct
     5161 agcattattt tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag
     5221 cggataaatg ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt
     5281 ttttttcttc ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa
     5341 tcattttctc acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa
     5401 actaaaactt tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag
     5461 aaacaaagag gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact
     5521 tttcgaatca tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg
     5581 atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa
     5641 gctcaaaaaa agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt
     5701 tattcttttt atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt
     5761 tttgtaacgg aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg
     5821 atttcgctct catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca
     5881 acaattacag ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa
     5941 ggcttatgac gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa
     6001 tatgtctcga tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca
     6061 tatcccggaa catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg
     6121 gtctttcctg gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata
     6181 aaagaagatt tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct
     6241 ggatattgtt ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc
     6301 tctctcctta acaaggtgat gaataa
//
LOCUS       DAYYCQ010000116         6321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000116 DAYYCQ010000000
VERSION     DAYYCQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.119"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHAYF_002675"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHAYF_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8580032.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACHAYF_002676"
     CDS             500..862
                     /locus_tag="ACHAYF_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8580033.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACHAYF_002677"
     CDS             914..2596
                     /locus_tag="ACHAYF_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8580034.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACHAYF_002678"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACHAYF_002678"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8580035.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACHAYF_002679"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACHAYF_002679"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8580036.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACHAYF_002680"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACHAYF_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8580037.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2105 a         1096 c         1436 g         1684 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata a
//
LOCUS       DAYYCQ010000117         6287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000117 DAYYCQ010000000
VERSION     DAYYCQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.120"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACHAYF_002681"
     CDS             190..690
                     /locus_tag="ACHAYF_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8580038.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACHAYF_002682"
     CDS             787..2634
                     /locus_tag="ACHAYF_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8580039.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACHAYF_002683"
     CDS             2637..3533
                     /locus_tag="ACHAYF_002683"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8580040.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACHAYF_002684"
     CDS             3583..3972
                     /locus_tag="ACHAYF_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8580041.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACHAYF_002685"
     CDS             3959..>6287
                     /locus_tag="ACHAYF_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8580042.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAYYCQ010000118         6223 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000118 DAYYCQ010000000
VERSION     DAYYCQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.121"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1467)
                     /locus_tag="ACHAYF_002686"
     CDS             complement(<1..1467)
                     /locus_tag="ACHAYF_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8580043.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTE"
     gene            complement(1765..2700)
                     /gene="murB"
                     /locus_tag="ACHAYF_002687"
     CDS             complement(1765..2700)
                     /gene="murB"
                     /locus_tag="ACHAYF_002687"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8580044.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2773..3693)
                     /locus_tag="ACHAYF_002688"
     CDS             complement(2773..3693)
                     /locus_tag="ACHAYF_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8580045.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3806..4558
                     /locus_tag="ACHAYF_002689"
     CDS             3806..4558
                     /locus_tag="ACHAYF_002689"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8580046.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5422..5955
                     /locus_tag="ACHAYF_002690"
     CDS             5422..5955
                     /locus_tag="ACHAYF_002690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8580047.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1870 a         1189 c         1039 g         2125 t
ORIGIN      
        1 ttcagttaaa tgtctatttt tttctctgat gatggtattt ccaaaatatt cccagtcatc
       61 tggagtcggt ggatttttcc aaattatctg ttctttatta aatttttcaa gtaaacaatc
      121 agataaataa atattcgtac gagaagtgat tcgactctca atcacaatat tgtaattttt
      181 aaatcctttc acttgagaaa taatgtaatc ggtataagca gatccttgcg aaaaatcaat
      241 acagacatga atcggtgctt caaaatatga taccggacaa ataccgacca ataaaaagag
      301 ataatcataa aataagcgaa tcatgaaacg ttcatctttt cccaagaaat agcgtttatt
      361 ctcttgtatg accttttgga ttgctgtact gaatattgga tacgtctcga agaaaaactg
      421 tagctgcttt ttagacgtaa agctagtcat ctcaaagtcg aatatccaat tttttcgatt
      481 gacatgcatc atacccatac gaagttcttt tcgtatatat agtctttctt cttcagagat
      541 ttcaatcgaa aatggtaatt gatcaatcaa ttccactgac attttttgac taatcatgtg
      601 gatatcatga aaatgagctt ccgcaagtcc aaatgcgcca tctaattcaa attcttcttc
      661 cgtgatcaaa aacaacatga tataagaaat ctcatcaatg atctgctctt tagggatatg
      721 aagaaaagaa atatgtgata aatccttgat gatcttcatt ccttctgagc tcttaaagta
      781 ttcatctgaa aagaaacgtt ttccgattgg ttgattccca taggccctca acaaactaat
      841 agtggttagc aaattgaatt tgattcgttt tgttgcagac agttttaaat tgaacagata
      901 ttcaaaatat tgcacgatac tctttgcttc attcatgacg ctttctctaa atggcataga
      961 aaaaccatta aagaccccga aatatacccc aaataatacg ttccgtatgt ttatttcttc
     1021 cccaactaat atgtttttct ttaactttat ttgatgctgt tcaagaaaag agactaattc
     1081 tcttcttttt acataggctc ttgatatgct taaaaaatga ttctctgcaa atttttcgat
     1141 cgttactttt gaatccacga tctccgttaa cagtcgagca atttttgatc gttgaaagta
     1201 gattcttctt aaatttttga tatccgtcaa attcagacgt tcgacatgga tctctgcttc
     1261 ttctgaaatt tggatctctg gctttgtttt gaattcagta aattctttat ttaattctat
     1321 gatataattt cttgttttaa atttagacaa acctaatgtt tcatataact taggcagcac
     1381 tacataattc tttttctcaa attctaaata tctagctagc agcagttttt ctctatcttc
     1441 ctcttctata aggtaatcca acttcatatc ctacacctca aaaaatgcct cataccaatt
     1501 acttgatatg cagatgaaag ttcatctttt cacctatact gattttatga tggattatgc
     1561 ttgttttttc atcagttatt ctcatttttt atcttttttt tgagaatatt gcaaaaaatg
     1621 gaaaaattta tgaggaaata ttctatattt tttgttttat tgtgttaaaa tgtacaagat
     1681 ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc ctgctctttt
     1741 acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt ctgatattgc
     1801 ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt tttctttgat
     1861 tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat gatcgatatt
     1921 gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca ggccttgcaa
     1981 tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa agacacttcc
     2041 gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact catccattct
     2101 tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca atacgatagc
     2161 acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt ttgtcaaagt
     2221 tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt tgatctctcc
     2281 gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga tgccgcaagc
     2341 aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa tcagtgaggc
     2401 acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt cagtcagcat
     2461 gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat ttcctaatac
     2521 catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt cttcttgttt
     2581 tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat agttcatcaa
     2641 tggttcatca aacaatatag taatcgtggc taagctttca actatttctt ctttattcac
     2701 tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt aatacaatct
     2761 tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct cacataattc
     2821 atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac ctaaacggat
     2881 ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg tgtttgtcca
     2941 gtcgccaaat tctttcactt tttttccatc agcatcttga atgacaagct gggtcagatc
     3001 gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat tagcgccact
     3061 tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta aaatggcata
     3121 ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg gttcatctaa
     3181 taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga actctgtcgt
     3241 ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg ggatacgttt
     3301 caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa ttatttggtt
     3361 cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca gtggtttgtc
     3421 aatataaaca tggataccat tttctagaca ttttttggct aattcataat gtacttttgt
     3481 tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga tcgtttcata
     3541 tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat ttcttgttgc
     3601 aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg ctttttctgc
     3661 gatattccct acaccgatta caccaatctt catctacttt cctcctggat catttagtta
     3721 ctttcatctt acaccatttt tttctaaaat gaggtacaat agtatcgcaa aggtaaggaa
     3781 attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa acgggcttcg
     3841 tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg attttttctg
     3901 tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag gttattatca
     3961 atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg ctaaaaaacc
     4021 agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag gccgtctgat
     4081 tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt ctcaaagcga
     4141 attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt atttggaaaa
     4201 tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc atcaaaagat
     4261 tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag aagaaagagc
     4321 ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt tttatccaac
     4381 ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa acaatgctgg
     4441 ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag ctgatgcaaa
     4501 gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt tgaaataaag
     4561 aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa aatggctttc
     4621 cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac tgtttatcga
     4681 gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc
     4741 tcatattttt cgtttggttg ggcttattac cgcagataca agcttttgaa caccgtttat
     4801 taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa caaaaatagt
     4861 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
     4921 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     4981 agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct tttgaacacc
     5041 gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa caaatggcaa
     5101 aatagttcct catattttgc tattttttga gctaataccg agggtcagct gattgaacac
     5161 cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa atttttttga
     5221 aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca tgatccccat
     5281 ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa atattacata
     5341 aaaaattcta tgttttttgt taaatttatt tgcttttttc aaaagtatgt tacagtaatt
     5401 tcccaaggag gttttacaga aatgaatttt gttgccatgg attttgaaac tgccaatcat
     5461 cagccttata gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat
     5521 gaattctata cattgataca gcctgagact ccttttttct ggcgaaatgt ccaaattcac
     5581 ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc
     5641 caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt tgatacaaag
     5701 gtcttagcgg gatgcttaga ttattatcat cttgaacagc ctaattatct ttctttatgt
     5761 acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa atcatcgatt gaacactgtg
     5821 tgcgaaaatt tgaacatcca attagatcat caccatgatg cattggaaga tagtcgtgca
     5881 tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga cagatccatt gaaaaaactt
     5941 gtgactataa agtaaaagaa tacttatgaa acacacaaag aggtcgtgag cctgccgttt
     6001 cactccgagt cacaaggagc aatttcaaaa aatagcttct catgtttatt aaatattgtg
     6061 actggtgacc gaggagctgg caggagaaca ccgtttattg gagaggttgt gaaaaagctg
     6121 ttctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt ttcatttggt
     6181 tggacttatt accgcagatg caagcttttg gacaccgttt acc
//
LOCUS       DAYYCQ010000119         6192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000119 DAYYCQ010000000
VERSION     DAYYCQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.122"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..165)
                     /locus_tag="ACHAYF_002691"
     CDS             complement(<1..165)
                     /locus_tag="ACHAYF_002691"
                     /inference="COORDINATES: protein motif:HMM:NF045046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HFM8580048.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSELAPALY
                     MCDEKIVLPKV"
     gene            complement(177..1994)
                     /locus_tag="ACHAYF_002692"
     CDS             complement(177..1994)
                     /locus_tag="ACHAYF_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8580049.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2008..2772)
                     /locus_tag="ACHAYF_002693"
     CDS             complement(2008..2772)
                     /locus_tag="ACHAYF_002693"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8580050.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2800..3498)
                     /locus_tag="ACHAYF_002694"
     CDS             complement(2800..3498)
                     /locus_tag="ACHAYF_002694"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8580051.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3510..4289)
                     /locus_tag="ACHAYF_002695"
     CDS             complement(3510..4289)
                     /locus_tag="ACHAYF_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8580052.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4305..5249)
                     /locus_tag="ACHAYF_002696"
     CDS             complement(4305..5249)
                     /locus_tag="ACHAYF_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8580053.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5295..6074)
                     /locus_tag="ACHAYF_002697"
     CDS             complement(5295..6074)
                     /locus_tag="ACHAYF_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580054.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1615 a         1292 c         1123 g         2162 t
ORIGIN      
        1 aactttaggt aatactattt tttcgtcaca catatatagt gcgggtgcta gttcagagca
       61 atctgctcca atgatttttc cttccatttc attattgttt aatgctgttt taaatgcatt
      121 cactagttca caacgacgtc cacaacttaa tattaaaata ttcaattttt aatctcctat
      181 ctattcgaag catttgcaaa agtgacaatg tctttagcca attgttcatc gtcttgcggc
      241 aggctcttga caaaatccat cactttttgt attgagtaac ctttgatgtt tcctacaaag
      301 attttctcat aaactgcttc atcatttctt tccttatcca acaatagttc ttcatatagt
      361 ttttcccctg gacggatacc tgtttcaatg atttcgattt cgtcttcgct atacccgctc
      421 aatcggatca tgtttttcgc taggtctaca atcttcactg gttcactcat atctagaata
      481 aaaatttcgc caccttttgc taatgcgcca gattgaataa ctaaacgact agcttcagga
      541 atcgtcataa agtatcgagt catacgaaag tctgttacag tgattggacc accttgagca
      601 atttgttctc tgaatactgg aatcacacta ccgcgagaac caaggacatt cccaaatctg
      661 acagctgaga atttcgtaca gccttcttca ttcaatccag tcacaatcat ttcagcgatt
      721 cgttttgttg aacccatgac atttggagga ttgttagctt tatctgtcga taccatgacg
      781 aagtttttca cttttgctgc tttagctgct tctgctacat tttttgtacc gaaaatattg
      841 tttttgactg cttcttttgg attatattcc atcagtggta catgtttatg agcagctgca
      901 tggtagacga tatctggatg atattgttcc atgatctcat tgattttctc gcgatcttgg
      961 atatctgcga tgatcggtac gatttctgta ggacaatttt gatgatgcgt gcgtaactca
     1021 cggtcaatca agtagatcga gttctcccca tgtcctaaaa gcaatagctt tgcaggattg
     1081 aattgaatga tttgccgaca gatttctgaa ccgatcgatc ctcctgcgcc ggtgacaaga
     1141 atgactttgt ctgtgatctg atctttgatg gattcaatat ccagctttac ctcatctctt
     1201 cccagtaaat ccactacgtc aattgttttt aattttgata cgctaatttt ccctgaagca
     1261 agttcttcta tagaaggcat cgtgttgact ttcacacgag cggattcgac taactcgaag
     1321 attcgtcgta atttttttct tgataaggaa gggatagcga tcgttaccat ttgaatgtca
     1381 tatttttcaa tcaattccgg aatatctttt aatgcaccta atactttttt tcctgaaaga
     1441 tacgtatttc ttttatttgg atcatcatct acaaacccga caacgtggat atcttgagcc
     1501 gttttggatc caagaaagct gttgtataaa atacgcccgc cttcgccagc accaacaatc
     1561 aatgtatttt tagcagaatc tgctgatacg tatctcatat tttttgtttc gatgtagata
     1621 cgccagatca atcggctacc gatgatcaat aagagcgaaa gcagataagc aaagataact
     1681 aaccgtaaac tgtatcgccg attaatgaac agcagtatga ggatacttga ggcagcactt
     1741 gcagatagtg aactaaaaat tgcaatgatc tctcttaaat ttgtatagcg attgatccga
     1801 gtgaaaactt tgaataaact accataaaat aaataaaaac caatcgacaa accgatagag
     1861 attaaaataa gatccattgg tatagataca aacggcttca tgaatttagc tgctgcaata
     1921 tttgcagcaa ttaataacaa gctgtccaca aaaaccaatg tggcaatttt tcttcttctt
     1981 gttaatataa acacttttta attcccccta aaataaacca aacttcttct tttttacctc
     2041 ttcatagtct ggataactga tttcatcccc gttgatcaga tcctttgcaa cttgcttcat
     2101 ctgtactact ttttccttac caaagtcctt ggcaatctgc tcatagcact cttttaagta
     2161 aaaggtgcgc ttagagactc cgtgtgcatc agaagcagtc atctgcacga gattatgttt
     2221 gaccattgtt ttagctgttt tctgaatatc tttgccaaat accccaacat aacttggcgc
     2281 agtaagttgg gccaaacacc ccatatctag gaacggaagc aaatgattcg ggtcttcgcg
     2341 aaaatgcgcg ttgcgttctg gatggacgat cactggtgtc tttcctagtt ctaacaactc
     2401 aaaaaaaagc cgttctgcat ataacggcac ttccagtgtc ggaaattcga tcagaagata
     2461 tgtatcatct aaatctgtaa ataaaatttc atctcggtgg atgtcttcga tgagcgttcc
     2521 ggtaatacgt acttcttgtc cttccaataa cgtcaaaggc aactgtcttt tctctaattc
     2581 ggcttgtaac gaagcgacta gagaaatgac ttgtgatttt tcattgctgt attttccatt
     2641 attgtggtgt ggtgtgcata aaatatgcgt gatgccttgc tgaatcgctt tttcagccat
     2701 tgcaatactt gcttctaagt tttctgcccc gtcatcgatt cctggtagga tatggcaatg
     2761 tagatcgatc atttctcttg tctcctctct tcttattcct taatttccgt agtaataacc
     2821 agcatctttg ctatgttctg ctccgttgta tacaacacct aaaactcgtg cttgtaccat
     2881 ctctagcagt tcttttgctt tattcaatga ttctttacgt gatgtatttt cacgtacgac
     2941 caataaagtg ccatccgttt ttgaagacat gatctgtgca tctgttacgg ctactactgg
     3001 tggcatatcg aatatcacca cgtcaaataa ctgtcttgct tcgtctagga tctgatccat
     3061 tcttggcgaa cttaacaatt ctgatgggtt gggtggctta ggtccactag gcaaaatcga
     3121 caagttctcg actggcgttc gttggattac atctgctaca cttcctgatg aactaagtgc
     3181 tgtacttaat cccctggcat tgttcagctg gaatgtctta tagacgacgg gtttacgtaa
     3241 atctgcatcg actagtagaa ctcgttgacc ggatttagca aaaacgactg caatgtttgc
     3301 tgcagtggtg gattttcctt ctccaggacc tgatgaagtc acgacaatcg ttttgatttg
     3361 ctgaccagct gcggaggcaa actggatatt cgtccggatc gtacgatatt gttcggcgat
     3421 tggtgaagat gggtttgtta atgtgatcaa actgactgca tgagtttgtg ctgctttttg
     3481 tttctgtgtt cgtgccattt ttcttcctcc tagactcttg agcggcttct gcgtgatggg
     3541 gctgttttat cgtttgtagc ttttggtcgt gcaggagctg ttcctttttt acggatacca
     3601 gcgttcaatt ctttcgctga catttctgga actgttccta agattgggaa tcctaatgca
     3661 tccgtcacat atttttcatc tttcactgtc cgatcaagta attcgaataa gaatgccaga
     3721 cctacgccta ccatcattcc taatactaag ccaatcacta gattcaattt attatttggc
     3781 gaaacaggtg tcgtattggc aactgcacct gaaataatcg agattttatc gacattcatc
     3841 acgtcttttg cgttctcttg gaaaacttgt gaagttgtgt tagcgatctg ttgagcagtc
     3901 actgcattcg tgcttattgc ctggatcgag aacatttgtg aattttgtga ctgattgacg
     3961 gagatcgcat ctttgatttg tccagcagtc attttcacat tgtcttctgt ttctaaacga
     4021 tctttgactt catttagtac taaatcactg acgatcatat ctttatacgt attgatcatt
     4081 tgcaggttcg tattgacatc attgacattt gctgtgtctg attgtggcag tgtcacgatc
     4141 aattgtgctt gtgaactgta ttttggtgtc aagatgaaaa atgttacgat tcctgagatg
     4201 gccaagccag caaacattga gatgatgatt gttaccaagt gtttgcgtaa tgtttcaaaa
     4261 atttctttca agctgattgt ctcttccatt ttttctccac tttcctattt attctctaga
     4321 ttcatttgat cttttaattc ttttttcaca cgatcaagtt cttgatcgct gactttttga
     4381 taggagatat tatcttgcat gaagccttct ccttgcagct gatcttgctt gatcgtacca
     4441 aatgcctccc gataatctaa agcgattttt ttcatttcat caaagctcat gtctgttttc
     4501 atattcgact ctagagcatt caaaacggat tgatagttgc tgacaccgtt taaactcaat
     4561 actttttttg ctacaccttc tacgatcttt ctttgacgct cttgtctacc atagtcacca
     4621 tttggatctt cataacgcat acgagaataa gataatgcct gatccccgtt caatgtaatt
     4681 ttaccgattg gaaagtcata gccatcttgt gagaaagtga ggtcattatt gacttcgata
     4741 ccaccaactg catcaacgag ttcctttagc cctttcatat tgatagagac atagtgatcg
     4801 attgggatgt ctacatattt ttgtagggta tccatcgcca tggcagctcc gccgaatgcg
     4861 taagcgtggt tgatcttgtc ttttgtgtca cgacctacga tgtcgacata cgtatctcga
     4921 ggaatgctga cgatcgttgt ctgtttgtct tcaggactga ctgtggcaac catcatcgta
     4981 tctgatcttc cttgatcgac ccgacctaaa tctccagtat caatgcccat caatagaaca
     5041 gagaaagatt cttgtttgtc cagatcaact ggtttcgttc gttctccttg ggttcgttcc
     5101 actgattcgt aagttttctc aatagaatgt gagagatcca gatataattt tgctcctact
     5161 cctacaattg ctaatactaa tacgataaga actgctaaaa taatcagaaa tatttttttc
     5221 cctcttgatt ttcttcttcg tcttgccata gtttcctccg tttgctaacc ttgcttgaat
     5281 caacgtttta actgtcaata acttatattg ctatagccca tttgctgagc aacagtttgt
     5341 gcttttgttg cgtatgctgc gcttattttt tcattccgga tctgctctat ttgactgagc
     5401 gccgtcaaat attgatcgta agtagcggtg gcagtcactt gaccatcctt caacatcgta
     5461 tcgaataact gatctagttg cgtgacttgc gcgatctgag tatcagcgaa tcctagatat
     5521 tgcataacaa ccgttttcca aggactatct ttaaaaaagc tcatattttc acgtacacga
     5581 gcgacatctg cacttgtcac cttttctttg atgacgacat cgtctacagc ttgctgccag
     5641 tttgatacat ttttagtaaa taaatcgttg acagctgtct ggatcttcag ttttgattct
     5701 gcactatcta actgtgtcac gattgctttc ttctcatctt ggattgcaac agcttcttta
     5761 ggcaattctg attctttgat gtcgtaatca tctgctgaaa gtttgacaga ggatagctta
     5821 tttgacaaac ttgtgattgt ttcttctgtt atttcagggt ttagaaaatt tttttctttt
     5881 gtactataca gcttgtctac ttcttttgat aaagaagtca aggtgtcttt ttgattggtg
     5941 atggcagatt ctgcataatt gatccgttgc tgatgttgcc gctgattatt gatatggtaa
     6001 ccaaacaaac agatcgcaat agcacctgca cctaccgcca cagcagtcaa tgacttgacg
     6061 aatggctttt ccatagattc actcctaagt attgatgata gataaattaa gatataattc
     6121 acatacataa aaatgacagc tttttatttt aaaaaacata attaaattaa tatattcaca
     6181 gaaatatttg ta
//
LOCUS       DAYYCQ010000120         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000120 DAYYCQ010000000
VERSION     DAYYCQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.123"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHAYF_002698"
     CDS             725..811
                     /locus_tag="ACHAYF_002698"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8580055.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHAYF_002699"
     CDS             complement(932..1621)
                     /locus_tag="ACHAYF_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580056.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACHAYF_002700"
     CDS             complement(1866..2780)
                     /locus_tag="ACHAYF_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8580057.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHAYF_002701"
     CDS             complement(2762..3142)
                     /locus_tag="ACHAYF_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8580058.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHAYF_002702"
     CDS             complement(3592..3879)
                     /locus_tag="ACHAYF_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8580059.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHAYF_002703"
     CDS             complement(3899..4123)
                     /locus_tag="ACHAYF_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8580060.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHAYF_002704"
     CDS             complement(4578..4748)
                     /locus_tag="ACHAYF_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580061.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHAYF_002705"
     CDS             complement(4763..5296)
                     /locus_tag="ACHAYF_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8580062.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHAYF_002706"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHAYF_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8580063.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYCQ010000121         6153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000121 DAYYCQ010000000
VERSION     DAYYCQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.124"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACHAYF_002707"
     CDS             complement(207..1169)
                     /locus_tag="ACHAYF_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8580064.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACHAYF_002708"
     CDS             complement(1171..2610)
                     /locus_tag="ACHAYF_002708"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8580065.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACHAYF_002709"
     CDS             2795..4741
                     /locus_tag="ACHAYF_002709"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8580066.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /gene="scrK"
                     /locus_tag="ACHAYF_002710"
     CDS             5005..5877
                     /gene="scrK"
                     /locus_tag="ACHAYF_002710"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8580067.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1953 a         1056 c         1089 g         2055 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 aggttctgtt gcaaagtttt aaatctacta tca
//
LOCUS       DAYYCQ010000122         6029 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000122 DAYYCQ010000000
VERSION     DAYYCQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.125"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..370)
                     /locus_tag="ACHAYF_002711"
     CDS             complement(68..370)
                     /locus_tag="ACHAYF_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8580068.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(590..956)
                     /gene="ssrA"
                     /locus_tag="ACHAYF_002712"
     tmRNA           complement(590..956)
                     /gene="ssrA"
                     /locus_tag="ACHAYF_002712"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1127..1900)
                     /locus_tag="ACHAYF_002713"
     CDS             complement(1127..1900)
                     /locus_tag="ACHAYF_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8580069.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2044..2394
                     /locus_tag="ACHAYF_002714"
     CDS             2044..2394
                     /locus_tag="ACHAYF_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8580070.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2375..3091
                     /locus_tag="ACHAYF_002715"
     CDS             2375..3091
                     /locus_tag="ACHAYF_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8580071.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3091..4539
                     /locus_tag="ACHAYF_002716"
     CDS             3091..4539
                     /locus_tag="ACHAYF_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8580072.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4610..5119)
                     /locus_tag="ACHAYF_002717"
     CDS             complement(4610..5119)
                     /locus_tag="ACHAYF_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580073.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5109..5834)
                     /locus_tag="ACHAYF_002718"
     CDS             complement(5109..5834)
                     /locus_tag="ACHAYF_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580074.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2052 a         1050 c         1170 g         1757 t
ORIGIN      
        1 aaaaatatcc cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta
       61 gcctacttta atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac
      121 gccgatgatc gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat
      181 ctttcttcat tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga
      241 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      301 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt
      361 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
      421 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      481 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      541 ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg
      601 gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac
      661 tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga
      721 tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc
      781 ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa
      841 gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg
      901 taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta
      961 gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat
     1021 tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa
     1081 aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa
     1141 aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa
     1201 gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca
     1261 aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt
     1321 ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg
     1381 agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac
     1441 ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa
     1501 tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg
     1561 tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat
     1621 catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta
     1681 ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag
     1741 agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta
     1801 atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc
     1861 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
     1921 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
     1981 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
     2041 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
     2101 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
     2161 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
     2221 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
     2281 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
     2341 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
     2401 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
     2461 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
     2521 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
     2581 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
     2641 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
     2701 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
     2761 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
     2821 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     2881 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     2941 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     3001 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     3061 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     3121 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     3181 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     3241 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     3301 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     3361 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     3421 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     3481 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     3541 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     3601 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     3661 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     3721 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     3781 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     3841 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     3901 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     3961 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     4021 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     4081 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     4141 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     4201 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     4261 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     4321 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     4381 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     4441 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     4501 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     4561 catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt tagcccgcgg
     4621 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4681 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4741 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4801 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4861 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4921 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4981 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     5041 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     5101 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa
     5161 gtgaaaggga atagactaag aatgagacag catccactga gcccaataag agtttgggca
     5221 aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc
     5281 tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc
     5341 agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac
     5401 tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc
     5461 tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc
     5521 aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat
     5581 agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga
     5641 agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct
     5701 aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt
     5761 gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag
     5821 cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt
     5881 atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga
     5941 ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat
     6001 tcgaaaagag gagcagcgac aataatttt
//
LOCUS       DAYYCQ010000123         5985 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000123 DAYYCQ010000000
VERSION     DAYYCQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.126"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..451)
                     /locus_tag="ACHAYF_002719"
     CDS             complement(<1..451)
                     /locus_tag="ACHAYF_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8580075.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            675..1466
                     /locus_tag="ACHAYF_002720"
     CDS             675..1466
                     /locus_tag="ACHAYF_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8580076.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1432..2385
                     /locus_tag="ACHAYF_002721"
     CDS             1432..2385
                     /locus_tag="ACHAYF_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFM8580077.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2783..3757)
                     /locus_tag="ACHAYF_002722"
     CDS             complement(2783..3757)
                     /locus_tag="ACHAYF_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8580078.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3872..4306
                     /locus_tag="ACHAYF_002723"
     CDS             3872..4306
                     /locus_tag="ACHAYF_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8580079.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4431..5453)
                     /locus_tag="ACHAYF_002724"
     CDS             complement(4431..5453)
                     /locus_tag="ACHAYF_002724"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFM8580080.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5845..>5985
                     /locus_tag="ACHAYF_002725"
     CDS             5845..>5985
                     /locus_tag="ACHAYF_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFM8580081.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRA"
BASE COUNT         1881 a         1129 c         1032 g         1943 t
ORIGIN      
        1 aatttgtaat aagttcgtat atgttttgcg gtgtttcttg tttcttgtaa ttttccacga
       61 atgtttcatc catgaataaa ttcataaacg cttgaagtaa tcctacttgt tcttttggtt
      121 ctttgattcc taaaaaaaat agattttcta cagtcatttc ctgattatcc cccatttgat
      181 aaaaataaat tgggttcttc agctgcataa caaaaacaaa tggcttttcg acatattcac
      241 attccgaatg tggcagcgcg atatttaaat acttagtaat caatccagta ggaaattctc
      301 tttctcttgc aatcagtcca ttcaaataat tctcattgac gtaccccatt ttttctaatt
      361 gttgtgcagc gattaaaaaa gcttcttctt tattttttac ttctaccgct aattgaatca
      421 aactaggaac gaacatctct ttatatttca ttccaacctc ctcctttttg attaagtttg
      481 ttaatttatt gttctactaa attcatcttt gttttacaag aacattatat ttctgttagt
      541 ttgttttgtc aacgctttca aaagaaattt attttgttaa atttgatcta cttgaaaata
      601 aaatcgttta tctatataat taagaagaac aaagtcgaac aaaaattgac ttttatgtta
      661 aaggagtaat tcatatgctg aaaaaagaaa gacaagaaca aatcattaca ttattagaga
      721 aagattttta tttaactaat tcagaaatcg ctaacaaatt aggcgtatca gaaatgacca
      781 tacgccgaga tgtcactgaa ttagcaaaaa aaaataaagt aaaaaagcta tatggtggcg
      841 ttgaacgaaa ggaaccttat aatcgagaac tatcgactca agaaaagatt gatacgaatg
      901 tagccgaaaa aaaatatatt ggtcaagtca tgaacagtat gattcccgat aattctacta
      961 tttatgtcgg tgctggaaca acgatcctat atgcacttcc tttcattcaa aagaaaaatc
     1021 tatttgtaat tacaaacagt ttgattgctt tcaactattt aaagaatcat acaagttatc
     1081 ggattttact tactggtggc gaattttctc ctattacaga agagttcatt ggggaagttg
     1141 cagaaagatc ttttgaaagt ttgaatgtcg atatctcttt cgctgcaaca aacggcatat
     1201 ttgataacaa tgtgacaacc gctcaattta ttgagggaaa tattcagaac gcagcgctca
     1261 atgcagcaaa aatcaaatgc attgttgcgg attattcaaa attaaataaa tcagacgtct
     1321 atacttttag aaaattaacc gattttgatt atctaataac cgataatcat ttatcagaac
     1381 aggatttcaa tcattattca aaatacttaa caatattaaa ggagcccaaa aatgatactg
     1441 acaatcacga tgaacccatc cattgattcc atttatttta caaaacactt tgaattaggt
     1501 acgatgacac gctttgattc tccggtacgc tatgtaggcg gcaagggaat caattgcgga
     1561 cgagcttctg ctctactagg ttcatcagtt attttaacag ggtttttggg cggttatttt
     1621 ggcagtatga ttcaagatat gctgaaaaat gaaaatttgt ttgatttgca ttttttacct
     1681 attgcagaag aatcaagagg tgccgtcacg atcatgcatg atgatgaaac gcagacagag
     1741 ctagtggaag ctggtcctga aatagcagat actactatca aaaaattaat gaatgatatc
     1801 aaactaatca gtaaaaataa ttacctttca gtcattacaa taaatggttc tgttaacaat
     1861 ccagatccct ttttgtatgc taaattatta caatatatca aagaacaaat agatccttcc
     1921 acctatatat taatggatat atctggacaa catctccgtg aagtcttagc aagtaagtat
     1981 aaaccgatgt tcatcaaacc taatatccat gagttttcag atatcatcca tcaaaaaatt
     2041 actcaaaaaa gcgacataaa acaccatcta aaaacaaatc cactatttaa agatatcgat
     2101 gttatcatga tttcttgcgg ctcgtatggt gcagtagtca aagtaaaaga agaattttgg
     2161 aatgttgaga tccctaagat tcctatcatt aatacaacag gctctgggga tgccactgtt
     2221 ggaggttttg ctcattcatt agaacagaat tatcctttaa aagaatgtgt aaggtacgca
     2281 atggcttgcg gcatgtcgaa tgcacagcaa tcagaagtcg ggaaagttat tccagctgat
     2341 gtaaaaaatt tagcaaaaag cattaaaatc acagaaatga aataaatagt acaaagtaaa
     2401 taattgttta tatgaaatga gacacaaaga gactgtgaca aaaaacgtgt gtaccgaccc
     2461 ccaaaagtta gattttttgg tctaactttt gggagtcgat acattgtcac agtccttttt
     2521 tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca
     2581 tgagaagcta ttttttgaaa ttgctccttg tgactcagag ttaaacggca ggctcacgac
     2641 ctcttggtat aaacggtgtt caaaagcttg catctacgct aatgagtcca accaagtcaa
     2701 aaatgaggag cttttttggc tgtccttatt acttgatgca gggcagtttt ttcataatct
     2761 cttttagatt aaatcctttt ggttacacaa ggttctcttc aaagaatgtt gccatttttt
     2821 caaatggaat gatgtctgtc tggtcgtata gatctgtatg ggatgctcct ggaatgatca
     2881 tgagttcctt gttgtcacct tttagttctt tgaaagcgtc ctccccaaag taacgtgaat
     2941 gagctttttc cccgtgaatg actaagactg catgacggat ttctggaata taatgcaata
     3001 gttttgcatt cataagtgac aaccctgatg tcctattcca accatcattg gagtttaaag
     3061 aacggacatg atatccgcgg tctgttttat aatacgcatg atagtctttg acaaattgag
     3121 gagcatcttc aggcaacgga tcaaccacac ctccagcacg tgcgtagtcc ccttgtttga
     3181 aatcaatgat gcgttgtgca tttaattctt cacgcatttc atatcgggca ttttcatcca
     3241 taacatcgaa gtagccattt gctgttactc gagtcatgtc atacattgtg gaaacaactg
     3301 ttgctttgac ccgcgtatcg atagctgctg cattcaatgc catacctccc catccacaaa
     3361 taccaatgat tccgattctc tcagggttta catttttttg tacagataaa aagtctactg
     3421 ccgcttggaa atcttccgta ttgatatcag gcgaagcaac atatcgaggt actcctccgc
     3481 tttctcctgt aaacgatgga tcaaacgcaa tcgttaaaaa gcctctttca gccatcgtct
     3541 gcgcatatag accagacgat tgttccttca ctgcaccaaa tggcccgctc acagcgattg
     3601 ccggcaattt cttttcactt acttcttttg gttcatataa atcagcgact aatgtgatac
     3661 caaaacgatt cgggaagctc acctttcgat gattgacttg attgcttttt ggaaatactt
     3721 tgtcccattc ttgtgtgatg tttaattctt tattcatatt ctagactctc ctttgatcaa
     3781 aattgacatt tgtttttgaa tgtattatta tgataaaact taaacttgac tttaggtcaa
     3841 ggtaaaaatt ttattttagg aggatttttt tatgtattcc attggacaag tgtctgaaat
     3901 gtttggattg cctgtttcta ctttacgtta ttacgacaaa gagggattat ttcctgatat
     3961 ccagcgtgtt tctggtatta gaaaattttc agagaatgag ttagaggctt taagagtcat
     4021 tgactgtttg aaaaaatctg gtttagaaat aaaagacatt aagcaattca tggaatggag
     4081 caaagaaggc agtaagacat ttgaggttcg taaagaacta tttgaaaaac aaaaagaagt
     4141 agtagaaaaa gaaatagcaa aattagaaag agtactcgct atgctaaact acaaaagctg
     4201 gtactatgaa gaagcaataa aagccggcaa tgaagaagcc gtattgacaa tgattcctga
     4261 tgatttaccc caacatgtaa aagaagctta tgtacattct cattgaacaa acggataact
     4321 cactctcctt ctctatgaac ttcatgaaaa catgaataaa cacctctaac tgattgttag
     4381 aagtgtttat tttttcactc aagtatcctg cacttacaaa aatatccttt ttaccgccat
     4441 cctaattctg gagcgatatc ttttacgatt gtttcaagca agtgcatgtt atattctacc
     4501 cccaaagtat taggaatcgt gacaagtact gtgtctgctt ctttgattgc ttcatcttca
     4561 gctagctcct tgattaattg atcaggttca ccagcaaaag tttttccaaa aatcgttggg
     4621 tttcggtgat aagcaagata gccgacagca tcttgttgcg gactaccgtc ttgtccaaat
     4681 aagcgtcgat ccaaatcagt ggtgattggc tgaatcgagc gagtaactaa tgttcttggt
     4741 tcaaaatcat gacctgcttg cttccacgct tctttatacg cacgtaattg gtttgcttgt
     4801 tggatatgga atggctcgtg ggattcgtaa ttgactaatg tagatgattg cagattcatt
     4861 ccatgttgtg ctgcccagat tgccgtttgc tgcgaaccag ctccccacca gattctttgt
     4921 cgtaatcctt ctgaataagg ttctatccgt aattttcctg atccttgtgg gaacattggt
     4981 tgaggatttg gttcttcaaa tggttttcct tcaagtaatt ccaagaattc aagggtgcgt
     5041 tcccgtgtta cctctttgat ttcttcttca ctgtaatcat aaccaaagta tttccagcca
     5101 tctagaactt gttcaggtga accacgcccc actcctagtt gcactcttcc ttgcgtgatg
     5161 atatctgtca atccggcatt ttctgccata taataagggt tttcatatcg catatcaata
     5221 agtcctgtac cgatttcaat cttacttgtc tttgcgccga ttgccgctag caaaggaaac
     5281 ggcgacccaa tctgaggggc aaaatgatgt acgcggaaaa acgctccgtc cactccgatt
     5341 ttttctgctt ccaccgctaa atcaatcgat tgcaaataag catcttgagc tgatttcaca
     5401 agcgatcctt gctccatcca atgtccaaag cttaaaaagc cgattttttt caaggtaatg
     5461 acctccttta aacctacaaa aatggaaggt tttttatttt taatatgaac tgattctact
     5521 actttttttc ttaaaagacg aataattgtt ctcgcctcac aatcaagaaa tgtatcggct
     5581 tactacaccc ctactttctt catcatctgc cgtgtcttaa tctcttttaa cagtctgtta
     5641 catcgttgat ttttgactca aaatgattgt ttctaacatg tatttatggt aaaaaagaca
     5701 agattagttc tttgtacaca tcgatttata cgctgaacaa aaagaaatat ataaactaag
     5761 aaacccattg ttttttcgct ccatcacata gagtttactt tagataaaca gtgacattta
     5821 ctaatccata acggaggaaa tcaaatgaaa aaaatctatt tcttatgtac gggcaattca
     5881 tgccgcagtc aaatagcaga aggctacgcg cgtaaatact tgtctccttc aatgtttgag
     5941 ataagaagtg cggggatcga gacacatggc ttgaatccaa gagct
//
LOCUS       DAYYCQ010000124         5982 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000124 DAYYCQ010000000
VERSION     DAYYCQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.127"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..1653
                     /locus_tag="ACHAYF_002726"
     CDS             16..1653
                     /locus_tag="ACHAYF_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580082.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1798..2538)
                     /gene="truA"
                     /locus_tag="ACHAYF_002727"
     CDS             complement(1798..2538)
                     /gene="truA"
                     /locus_tag="ACHAYF_002727"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8580083.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2729..3364)
                     /locus_tag="ACHAYF_002728"
     CDS             complement(2729..3364)
                     /locus_tag="ACHAYF_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8580084.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3511..4731)
                     /gene="sstT"
                     /locus_tag="ACHAYF_002729"
     CDS             complement(3511..4731)
                     /gene="sstT"
                     /locus_tag="ACHAYF_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8580085.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4950..5948
                     /locus_tag="ACHAYF_002730"
     CDS             4950..5948
                     /locus_tag="ACHAYF_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580086.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
BASE COUNT         1936 a         1123 c          902 g         2021 t
ORIGIN      
        1 aaataggaga aaaaaatgaa aaaaaacaaa aaccaaataa tagatatcat ttttatgtta
       61 tttctaagtt ttgcttacgt catccctcta ttcatgtcta agggaatcta tcatagtgtc
      121 aatcaggata cttactttca tttatctcga atcataggac tagataatgt atggagttct
      181 ccagtaaact tcaataactt cgatcatcat ggaacaatga tgaatatttt ttatccatgg
      241 cttactcttt atccagcttt tttattttat aaaatgatgg gaaatcttgt tttaagttat
      301 aatatctact atttttttat cactttttta acaatggtcg tttcttattt ttctatgaaa
      361 caaataaaaa acaatcgtta tatttcgctg ttattttcga ttatttatac attttccgcc
      421 tatcgtgcca ttgacatatt ccatcgtgct tcattgggag aagctgtcgc tttaacattt
      481 ttgcctttga tactaatggg gtgttacgaa atttatatta gagattacca aaaatggtac
      541 tggttgtcta ttggcatgac gctagtagtg tatactcatt tactttctgt cgctatggtt
      601 tctgtattta ttggaggaac gttgtttctt tctttttatt tttgggacca aaaaatcgct
      661 agactcttaa gtcttttaaa agctaccgtc ctcacctttt ttttaagtgc gggattctta
      721 attcctttta ttcaacaatc tagagcacaa gaattgaaag tcccactggg aaaagaatta
      781 agtggtatgg ctcctagtga tatgctgaca catattttaa ataataacta taacaactat
      841 actattggat tattcttatt tctcggattg attggtgctt ttatttttat aaaaaaatta
      901 actgctgatg atttatttat tttctttctt ggtgtatttg ttcttttttg ttcaaccaat
      961 ttatttcctt ggcaactttt taaccataca ccagttaaga gtctacaatt tgtctggcgg
     1021 ttaaatggtt tttcttcttt atttatagcc tatacgatga gtatagtgat ctattatatt
     1081 ttttctacta aaaataattc atggaaaata atggtcttta cgtttcttgc actaatattg
     1141 catctctcag gagtaagcaa ttctatccat gctaacaaag attcattaac acttatagca
     1201 cctgaagacg ctgttgctat agctgaaaat tacaatcata ctgattatgc aaacaaagag
     1261 tcaatctatc atcctgatat gattaataat gatcaatatt tactaggtaa ccaggaaata
     1321 aatccaacaa ctcattttat gagtaacaaa cttacaattg aattagataa ttcgaataat
     1381 aaaaatactg tcttgacgac acctatttat cgttataaag ggcaagtggc ttctattaat
     1441 ggcaaactag tacaaacaaa attatctaag tttggcacaa ctgaattaac tattcctcca
     1501 ggtataaata aagtcgttat tacttatcag tacacaaaac tagcaattgc ttcaagatat
     1561 ttatctatcg ttactttgat tttattttta ctttatcgat ttactttttc taaacaaaaa
     1621 caaccgagaa gagaagtaca acattctcac tagattttta tttaagaaag actttttagc
     1681 tgtacacata aacaggctaa aaagtctttt tttatatttc tttaatttgc ttcatacatt
     1741 atttaacatc gatatttata ctgtgaaaca tgcgtttcat ccattatcaa agtattttta
     1801 tcgataataa acttcatcca aaaacagacc ttgtgcaggg acggtttctc ctgcatgttg
     1861 ccgcactttg ctttcaaata tttcatcgat tgtttctatc ggcatggcac ctgtgccgat
     1921 ttctagtaat gtccccatga taatccgcac cattttatat aagaatccgt ctccgacaaa
     1981 agtaaaatga agcatttctc cctttcgttc gatagataat tcatcaatcg tacgcactgt
     2041 ggattttttt gattttttca atgcagaaaa accaatgaaa tcatgtgtac ccactaattt
     2101 ttcgcaagct gcattcagct tgtccatatc caactcttga gggaaataaa agctgtggtt
     2161 tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa tagctgtatt ttttgcccac
     2221 cgcattatat cttgaatgga agcgctcagg catttcttcg attcttttga tgacaatatc
     2281 ccgcggaagg taacgattca taaagtcgat catttctgcc agtccaattt ctgtgtttgt
     2341 tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca tctgttcttc ctgatccaat
     2401 aatttctatc tcttcatttg tcatctgatg caatactttt tcgatttttc cttgaatggt
     2461 tttatcgtca tctccaagtc tttgccatcc tgaatatctt tttccgtcat attcgattgt
     2521 caacttgatg tttctcatat tttagtggct cctctgtctt ccatttggtc ttctctctac
     2581 ctttttaaga ttgtagcaca aaatatagct ccttgcttct ctaagacaga agctatacaa
     2641 gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca gccaccggat gaagtatccg
     2701 ctgactgttc aatttgacgg ctcaagtttt actgtttgag atgcaagata ctcaaccagg
     2761 caatgggcac ataattaaag aaaaaactga ttctttggca aaaacctatt ttattcagta
     2821 atggccaaga atttatagaa ggaattcttg ccagtccata aatcgccgca aaaagcaaac
     2881 tgatcacaga cagtccaagg tataagtggc ttacgtccat ttgcccattt tttcgataga
     2941 tcaagaaaag aagaactgga aacaataaaa aaccactgta acctaaacct gatccgatat
     3001 tatggaccca tgtcgagaca ttccacgaag attcatgggt attgatactg aacaaacctg
     3061 taaagataca atcacctata ccatagaacg cgatggctgc tgtaactagg gtagctaaca
     3121 cacgagatgt actaacaaat gcagcataca atgcaggcaa tgacaggatg aaaaagatcc
     3181 ctgaaaccac agaccaaata agaaacgctt ttcttacagg actggaaacc tctccaaata
     3241 cactgatcac gctcttcatt tgatctaatt caggataata caatcctaat acgtacggag
     3301 tcaaaaaatc actcagcacg cctaataata aaaagaaaaa accaaattta cgtaaaaaat
     3361 ccatgatacg cctccaataa caattaccta tgcttttaat tgttattata aacgacttta
     3421 tcttgattta acagaaacct ctatttacac gaacatctat ataactcatt aaaaactcga
     3481 atcattttaa aaatactaaa tatctaacgt tcaacgttca cttaatccat ctaaacaatc
     3541 tttcaatttg attttttccc cacttctttt acgcgcgcct agctctccta ttgcagtaaa
     3601 caacagatca cttgacgaat ttaaggcagt ttccattgaa tcctggataa caccgataat
     3661 aaatccaata ccgacgattt gcatcgcgac atcattagag ataccaaaca aactacatgc
     3721 taatggaatc aataacagtg aaccgcccgc aacacctgaa gctccacaag cagctaaagc
     3781 agacaaaata cataaaagga ctttcaaaac aaaaggtgct tcaatcccta aagaatgtac
     3841 agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg cccatattga tcgtagcacc
     3901 taacgggatc gatatggaat aagactcttt tgtcaatttc aatctttcag ccaacatcat
     3961 gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa gcgggaagtg cgctttcttt
     4021 caaacaaaag aaaatcaatg gatagggatt ttctttaata aacaaaaacg tgaggaatgg
     4081 gtacacaact aatgcgacaa acagcatcgt accgatcaaa acaagtaaaa gctgcagata
     4141 ctctgccaat ccagcgattc cagtcgtagc aattgattga taaaccaatc ctaaaatccc
     4201 tatcggagca aatgcaataa tcacttgtac gacttgtgac aacgctatag atatctgatc
     4261 caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct agtcccaaca atacagccca
     4321 gaataagacg ccaaggtaat tcgcatcaat aagagattga acaggatttt gtgtgacatt
     4381 cgtcaacata gtagataaaa cactttccaa actttgcggt gctttttcag atactgtttc
     4441 ttgtaaagta ataggaacag taaaaattga acttacaata actgctacaa gtgcagaaag
     4501 caatgttccc actaaatata agatcaaaat aggcttcacg aatgtcttat tcccaatttt
     4561 atgcttggca attgacgcca tcgtcaagaa aaaaaccaga ataggtgcga ttgccttcaa
     4621 gcaactgaca aacaacgttc ccaatattga aataaatgtc catgtgggga aaaagatacc
     4681 aaacaatatt ccgactaaaa taccaacaac aatacgttga actaacgaca tatccaaaaa
     4741 cttctctaac aaatgaccac cctttcacaa ttttaacaat cattgtatca ttttatgcaa
     4801 agaaactaaa ggtataattt tctaaaaatt ctaacaatac aaacaaccat ttcttgctaa
     4861 ttttgtactt tttctgtcta cacgttgttt tttgagatat tctaagttag gatgttatta
     4921 atgggcaact tatcaggaaa cgttttctta tgtttttgtc caattttttt gtgaggtgtt
     4981 tattcttgaa aaaaataaag aaggagagtt atggcttcct gacaatcttt gtcgcttgta
     5041 taatgatggt tttacagcgt atattgattt cagcagtcac tattcctaca tatgcaacaa
     5101 accctggtcg attctacatc tatacatttt tacaaacttc cgttgttatt ggtgcaaatt
     5161 tgatcccact ttacatgggt taccaatatc aaaaaatcgc ccgtttaaaa gttttacatt
     5221 atttaagtcg ttttgccttt atattcctaa tagccacact agtttgcaat atcttttttt
     5281 acgtacaagc tggcagcttg aatattcgtg attattggct tatcctaaca cctatcagtc
     5341 agaattactt tacttatgct gtttcttgta ctttgctttt ttgtagtatt ccttatacag
     5401 ttggattttt aaataaatta tcgaaaacaa caattaaata tctactatta tttttaacaa
     5461 ttggattagt tggctgctct actctattta ataaagaccc gtttgcatta cagaatggac
     5521 agagtatttt atggattttt tatttatttt taatgggtta tggtttgaaa gaatggaact
     5581 ggaaagaaaa ggtccgctat aagcttcctc aagtatttat cagcttgatt gttttattcg
     5641 gtttgattat tttgatgaca caaatttcac tcatcatcag aggaaataca gatacagcgc
     5701 tgaggttttc taaaccatat tctctcttcg caatgtatta tactttctct tcgtttttat
     5761 ttttagaaat gctatctgat aaattaggta taaaaggaag aggaaatttt cttgctgtat
     5821 ttttgattgt cactcaagtt atatgtaatt tccctttagt ttcttatcgc gtatctacat
     5881 ttatgaaacg tgaatttcca aactcaggga aggcatggct gataaatatc gggcagtttt
     5941 ttatttgata tttattagca gtgtccctcc tagttatctt tt
//
LOCUS       DAYYCQ010000125         5974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000125 DAYYCQ010000000
VERSION     DAYYCQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.128"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..415
                     /locus_tag="ACHAYF_002731"
     CDS             59..415
                     /locus_tag="ACHAYF_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8580087.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            405..671
                     /locus_tag="ACHAYF_002732"
     CDS             405..671
                     /locus_tag="ACHAYF_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8580088.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            830..1831
                     /locus_tag="ACHAYF_002733"
     CDS             830..1831
                     /locus_tag="ACHAYF_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8580089.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2201..2815)
                     /locus_tag="ACHAYF_002734"
                     /pseudo
     CDS             complement(2201..2815)
                     /locus_tag="ACHAYF_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3055..3300
                     /locus_tag="ACHAYF_002735"
     CDS             3055..3300
                     /locus_tag="ACHAYF_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580090.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3398..3619
                     /locus_tag="ACHAYF_002736"
     CDS             3398..3619
                     /locus_tag="ACHAYF_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580091.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3941..4621
                     /locus_tag="ACHAYF_002737"
                     /pseudo
     CDS             3941..4621
                     /locus_tag="ACHAYF_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4761..5300
                     /locus_tag="ACHAYF_002738"
     CDS             4761..5300
                     /locus_tag="ACHAYF_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580092.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT         2199 a          820 c         1070 g         1885 t
ORIGIN      
        1 aattcgaatt tttatgttat atactataat ataaaatcaa tatatgatat aattgggtat
       61 ggaaaaaata tcttttgaaa catttaaaag acctaatggt catgatgaat ttttagaatg
      121 gttggaaaca ttaccaaaaa aagatagtgc gaagctttta cgtacaatag aagaaacaga
      181 aaagaatggg atgctagtag ctcaacggtt gaaatgggtg aaaaaattag acactaattt
      241 gtacgaatta agaagtaagg taggatcaaa catccaaaga gctatctatt tccatgtggt
      301 caatggaagg tatgtcatta cccatggatt tactaaaaaa acggagaaaa caccaataag
      361 tgaacttaaa catgcaaaag aacttagaga ggagtggttc acaaatgatg attaaaaaat
      421 tgattgaaga aaagcaaaaa gacaaagatt ttgcagaagc atatgaccgt gaaggaagaa
      481 aactcgaaac tgcggttgct ctttatcatg cacgggagga agcaggccta actcaagcgg
      541 agcttgcaca aaaagcacat acaactcaat ctactattgc tcgaattgag agaggggata
      601 acgtatcttt cgataaactt gatgatatcg ctagggcatt aggcaaaaag ctggtagtag
      661 aatttgctta aaagcgagtt gaaaatatac ctgtaatagg ctcctaacta atcaattagt
      721 ttagtgggcc tttttgtact aaatagataa aagttgtcct atagtgtaaa agtcaataaa
      781 ttttatacaa aaattacttt aaagagacat aaagagaaag gtacaataaa tgaaaaccat
      841 aaatctaata aaagtattta tagcatcacc aggtgatata gcagcaaaga ggaatgaagt
      901 agaagaaacc atatatagat ggaatataga aaatacagat aatacaaaag taattttgat
      961 gccaattcga tgggaaacga atgcgattcc tacttatata aataatagct ctgggcaaag
     1021 tgttattaat aaacaaattg tagaaaatag tgatatatta ttagcgattt ttggccataa
     1081 gttgggttct ccagttgata attatagatc gggaactata gcagaaattg attactttta
     1141 taaaaatagc aaaggcgagg taggggtttt tttcttagat gaaccagttc cacaagtttt
     1201 ggctgaagag tatatagaag ttaagaagta cagggactct ttagaagata agggttttta
     1261 tggaacatat agcaaagaca atatcagaag gtttctcaca aaaaaagtga attatttaat
     1321 tgagaaagca caaaataatt tagaatcaaa acgctttgat ttagattcta tttcttacaa
     1381 gacaccttca gtaattaaca aaaaagttac cggaactgat aaagattttg atgacaactt
     1441 agatattttt gatccaatag aatttgacaa tgatgaaaga ttgctgctaa tatatgcgtt
     1501 tatggatgaa aattctatct tttcaactga taattcaact tatagaaaag tagttcaatg
     1561 ggaaagtgat aacaatttaa ggagttactt gagtgataga gtatttgatg tactagaaaa
     1621 attaagtaaa tcgggaattt taaaggaaga atacgattct tcttttgatg agtatccatc
     1681 acgctatatt ttgatttcag aggactataa gaaatttaaa aggtattttt taaaaaatga
     1741 aaaaaagata tctgttatta tggagaaata caaagctcca atacaggctg atccattttc
     1801 caatttggat gaatcagatt taccgtttta aattttaaat agataatttc attttttagt
     1861 agcagaggta taaacttttt taatgtttga attgaaaaga aatgctaata ggtcgacttt
     1921 atgtttctct tgtgcaatcg taaatgcggg ggaccataaa attgatctat tatcgagtgt
     1981 gtataaaata aaattcaaat cttattagtt tacataagat acattatact aagttgtcaa
     2041 tgttaaatgc ctttaaataa ggacgattta tgcatcaatt atatttatgt taactaaatt
     2101 ctttttaaca taaatgtaat caacttagct agatgaatct aaaatgattg tctgtattga
     2161 tgacaaagct aattaataat tacgtgattc aaaaaagaaa tcatacaatt agttggtcga
     2221 tttctttttt tattcggtaa attgtttgcc tagtcacatt gtagtctgtt gcaatctttg
     2281 agatagcaac cccattctca agatcctgaa cgatactcct atagacaagg cgacgttgag
     2341 gatctttggt gtctgcactg tataacttag gacgaccttt atagacgccg tttgctttag
     2401 cgattttaat tccttgtgcc tggcgtcgtt tcgattcagt acgttcttgt tcagcaatca
     2461 tggctgagat ttaaatgatt aagtctttga tgaatttatc taggagagga tttccaattg
     2521 cttcagccat gatcggtaaa ctggtgataa taagtcgcac attcttcttc tttagataat
     2581 tcactgaatc gataatctcg tcataattgc gtccaagtcg atcaatggct tctacaataa
     2641 aaatatcttg ctctctgaca aaatctaaag cttcctggaa aatcggccga tgactaatcg
     2701 tcgctccaga ttgtttttcg ataaaaattt tttcagcgcc aaactttttc atttcttcaa
     2761 tttgacgttg ttcattttga tcgatggatg aaactctaat ataagcaatt ttcaaattta
     2821 ttccctactt ttctataacg tttataatac accctaatca tacgttagaa ctctaaattt
     2881 ttcaatgtta tatttttgtc tatttagggt aaactctaaa tagacaaacg gtttttgaat
     2941 tctaaaagtt aaatagttta taatagaaga aaaagaatgg ggtgacgatt tcttagattg
     3001 aaaaggaaag ataaatatta tagattatta cttttgacag ctgaagcagg atggatggat
     3061 tttccagaag ttgtaaaaga agttcaagag ataggtgcaa ctatttctga tagccgctcc
     3121 tctcctatga actatcagga tgcccatgga attaaaataa ttgatcaaga gagaaatgtc
     3181 agaaaataca caacaataaa tgagtgtgta attaatgaaa atctttgtag ggcaccgata
     3241 acgaaacaca ttaagaatag aagcaaagca aaagacggaa gaacttttac taccttctaa
     3301 gtttttttca tatttgtttg ctatacttag atctaaggaa ttagactttc gctgcccatt
     3361 atttaagttt gaggtagcgt cttgcgaggg atcaataatg gaagaagaaa aaatatttga
     3421 aaaacgctgg gaattggctt cagttgaaca aagagcaagg taccataatc taatgtcctc
     3481 ttaccgtaat attgattgga cttacaaaga aaagaagtac ttgctttggt tatgtcagtt
     3541 agatgtaaat acctttgaaa catttgaagt gattctagat aagattaaaa acagcaatga
     3601 aaaaagagct gatctataat ggttcgctct ttttatttta ttaactcttt ggttactata
     3661 tgtaaaatac agcttcatat tacaataaat ttcaggatac aaatttattt aagcatatat
     3721 aaatataatt gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat
     3781 aaatgggcaa accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag
     3841 ttctgggaag aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt
     3901 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     3961 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     4021 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     4081 ttatcgttgg gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     4141 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     4201 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     4261 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     4321 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     4381 tgtgggttta tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga
     4441 acaagaccat cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc
     4501 ttccacgatt aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg
     4561 aacgctcttc ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta
     4621 agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa
     4681 ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga
     4741 aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg
     4801 tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag
     4861 ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact
     4921 atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg
     4981 gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag
     5041 aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga
     5101 aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta
     5161 cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt
     5221 tattaattag ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg
     5281 gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt tcatagcaat
     5341 ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc
     5401 gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt
     5461 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
     5521 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
     5581 tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc
     5641 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
     5701 atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
     5761 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
     5821 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
     5881 tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata
     5941 ataaagtact acctattaaa ggtagtactt tatt
//
LOCUS       DAYYCQ010000126         5814 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000126 DAYYCQ010000000
VERSION     DAYYCQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.129"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..710
                     /locus_tag="ACHAYF_002739"
     CDS             <1..710
                     /locus_tag="ACHAYF_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFM8580093.1"
                     /translation="LSRNKKYIENETDSVVLVSSEKVIDICENKILTSAFFEQNGFKT
                     PEILNSSEITKFPVFIKPLNGSSSVNTFIVNNEKELLFFSEYIDNPLIQEYILGEEYT
                     IDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIKDRELIETTKKMLEKLNAIGHITIQ
                     CIKNEKGIFYLEINARYGGGAPISIKSGANSPEYIIKMIDSKKNLRYRENYLDKMIAL
                     RYDQAIYLDEKGNVYND"
     gene            703..1398
                     /locus_tag="ACHAYF_002740"
     CDS             703..1398
                     /locus_tag="ACHAYF_002740"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8580094.1"
                     /translation="MIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIFPATFDEI
                     YRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLENADTLLTE
                     LRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLE
                     KFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIR
                     SLWEIKNFLGVIS"
     gene            1395..2513
                     /locus_tag="ACHAYF_002741"
     CDS             1395..2513
                     /locus_tag="ACHAYF_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFM8580095.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            2503..3123
                     /locus_tag="ACHAYF_002742"
     CDS             2503..3123
                     /locus_tag="ACHAYF_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8580096.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            3319..4419
                     /locus_tag="ACHAYF_002743"
     CDS             3319..4419
                     /locus_tag="ACHAYF_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8580097.1"
                     /translation="MAKYYNKKILIMANNDDGLYRFRGMLIKNLVDQGNEVIVSLPKG
                     KNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNYCNILKKIKPDMVIAYTIKPNIYG
                     GLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQ
                     TLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNL
                     YSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHE
                     GMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYE
                     QRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            4426..4821
                     /locus_tag="ACHAYF_002744"
     CDS             4426..4821
                     /locus_tag="ACHAYF_002744"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8580098.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            4818..>5814
                     /locus_tag="ACHAYF_002745"
     CDS             4818..>5814
                     /locus_tag="ACHAYF_002745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8580099.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDIIQNLSDEYYTRAICKCKYKDNWKGISAKFALKNKWVFLIYIYNKLIK"
BASE COUNT         2271 a          677 c         1086 g         1780 t
ORIGIN      
        1 cattatcaag aaataaaaaa tatattgaaa atgaaacaga ctcagttgta ttagtttctt
       61 cagaaaaagt catcgacatt tgtgagaaca aaattttaac ttctgctttt tttgaacaaa
      121 atggttttaa aactcctgaa attttaaact cttctgaaat tacaaaattt cctgtattta
      181 taaaacctct gaatggtagt tcatctgtta acacatttat tgtaaataat gaaaaagaat
      241 tgttgttttt tagtgaatat atagataatc ctcttattca agagtatatt ttaggtgaag
      301 aatatactat agatgtttgc ttaaactttg attcgacacc agtaagtatt gttcctagaa
      361 aaagaatagc aactagaggt ggggaaatac tgaagggtca gattattaaa gatagagaac
      421 taattgagac tacgaaaaag atgttggaaa aattaaatgc aatcggacat attactattc
      481 aatgtataaa aaacgaaaag ggaatatttt atttagaaat aaatgcaaga tacggtggag
      541 gagcccccat tagcattaaa tcaggagcta attcccctga atatattata aagatgatag
      601 attcaaaaaa aaatttgaga tacagagaaa attatttaga taaaatgata gcactgagat
      661 atgatcaagc gatttattta gatgaaaaag gaaatgtata taatgattaa agcggtgatc
      721 tttgatttag atgacactct tgtgtctgaa tatgattatg taaaaagtgg atacaaatgt
      781 gtagctagaa gattagcagt gcaaaaaatt ttcccggcta cttttgacga aatatataga
      841 gtccttttag atctttttgc tgaagatagt aaaaatgtat ttaatcgtct atatgactat
      901 tttgaaattc cttatagtaa aaaagaaatt aaagaactgg taaatgtata tcgagaacac
      961 aaaccagaca taaatttttt agaaaatgca gatactttat taacggaatt aagaagtaga
     1021 ggatacaagc tggggattat tacagatggg tatgctataa cccaacgaaa caaattggat
     1081 gctttgaaag tagactattt ggtagattat atttgtatta ctgacgaatt aggccgtgaa
     1141 ttgtggaaac cacaccctat ctcatatctt ttattgttag aaaagtttaa tttaaaggca
     1201 caagagtgta tatatattgg cgataatgag ttgaaagatt ttataactgc caaatcaata
     1261 ggaatgcaaa caattaaaat agaaagagta aatggtattt acagagaaag cagtttgaag
     1321 ttcaaaagat tttaccaagc tgataaaaaa atacgttcgt tatgggagat aaaaaatttt
     1381 ttgggagtga tttcatgaaa agtaaaagaa ttctattagc ttctccacat atgagtgaag
     1441 aaggatatga aaaagagtat gtaaaagaag catttgatac aaattgggtt gctcctttag
     1501 gtgcgaatgt gaatggattt gagaaagaat tagcagaata cgtgggagct agtcatgctg
     1561 ccgcattatc ttctggaaca gcagctatac atttagcttt gaaagcggct ggggtcggta
     1621 aagatgacat agtgatttgt cagtccttga cgttctcagc tactgctaat ccaattctct
     1681 atcaaggagc tatacctgta tttgttgata gtgattacga aacttggaat atgaaacctg
     1741 aacatttgcg aacagcacta gaaaaatatc ctaacacaaa ggctgtatta gtagtacatt
     1801 tgtacggttt atcagctaat ttagacgaaa ttactaaaat atgcaaagaa tacgatgtta
     1861 ctctaattga agatgctgca gaatcattag gaacaacata taaaggaaaa tatactggta
     1921 catttggcga ctatggaatt tattctttta atggaaataa gataatcaca acttcaggtg
     1981 gcggaatgct tgtatccaat gacaaagaaa aggtggaaaa agtgagattt tgggctacgc
     2041 aatctcgtga gaaggcacgc cattatcaac acaatgaaat tggctataat tatcgaatga
     2101 gcaatatcgt tgctggtatc ggtcgaggac aattgaaagt attaaatgaa agaattgaga
     2161 aaaaacgata tattcatagt ttttataaaa aagaattgtc taaattgtca aatatacaat
     2221 tcatgccaga aaataattgg aatgaaccga attattggtt aagtgtcata acaataaagg
     2281 gaataaatgc gaatcatctt attgatgagt tagacaaaaa taatatagaa gcccgcccag
     2341 tatggaaacc tatgcattta caacctgttt ttaaaaatta tgattatatt ggcgatgatg
     2401 tagcagaaac tttatttgac ttaggagtat gtttaccatc agatacaaag atgacagatg
     2461 aagatttaaa atatgtagta tctacgatta aaagagtgat caatgatgcg taaaaaaact
     2521 ttctatcagc tttttgggaa aagaatttta gatatcctat tgtcaggaat tgctctcatc
     2581 gttttatcgc cgataatctt aattgtaggt tttttagtaa gaattaaact tggttctcca
     2641 attatcttca aacaagaacg accaggaaaa agtgaaaaaa ttttttccat gtataaattt
     2701 cgtacaatga ctgacgaaag agaccacaat ggagaatatt tacctgatga aattcgacta
     2761 actaaatttg ggaaaatgct tagagcaaca agtctggatg agctacccga actttggaat
     2821 attttgaaag gtgatatgtc gatcgtagga cctcgtccat tattagtgga gtatttgcca
     2881 ctatatagtg agaagcaacg gaaaagacac aacgtaagac caggattaac tggactggct
     2941 caggtcaatg gaagaaatgc tattagttgg gaagaaaagt ttgatttaga tgtttattat
     3001 gttgataaaa ttagtttctt taatgatctt attattataa ttcagacatg taaaaaagta
     3061 atcaagaaag agaatattaa tacaatcaat aacaatatcc cagaaaaatt taaagggagt
     3121 taaatacagc agcttaatgc aagttttgtg acttgcatta tatttaggta taaatcaaag
     3181 cgagtatatt agaattatat aacatatgtt aaaaaaacgt atagctaata aaagtatgat
     3241 tttaaaaatt tgtgtagagt tttaaatata ctgtttcaat acgagaaaat tataaaaaag
     3301 tataaggaaa agagagttat ggcaaagtat tataataaaa aaattcttat aatggcaaac
     3361 aacgatgatg gtctttaccg ttttcgagga atgctcataa aaaacttagt agatcaaggt
     3421 aatgaagtta ttgtatctct tccaaagggc aagaatattc ctaaattaat tgaacttgga
     3481 tgtcaaatta ttgaaactcc aatagataga agaggaatta atccaaaaac tgactttaaa
     3541 ctatttttta actattgtaa tattttgaaa aaaataaagc cagatatggt cattgcttat
     3601 actatcaaac caaatatata cggtggattg gcatgtcggt taagaaaggt ttcttatgca
     3661 ttaaatataa ccggattagg aacggcattt caaaaaaagg gggttttgaa aagtcttatt
     3721 gtatttttgt atagattagc agctaaaaaa tctaaggtca tatttttcga aaattttgaa
     3781 aatatgaaaa catttcagac tttacaaatt gctaaacaaa gtaaatacaa gctattaaac
     3841 ggtgcaggag tagatgtaga acactttagt tatttagaat atcctaaaga tgagcaagtt
     3901 cattttttat ttattggaag aattatgaaa gagaaaggtg tagatgaact ttttgaagca
     3961 atgaaaaact tatatagtga agagaaaaat tgtgtacttg atgtccttgg gtattttgaa
     4021 gaagattacg gcataaaaat tgaaaaatac gaaaaagagg gatggttaaa atatcatgga
     4081 tatcaatctg atattcgtcc ctttgtagaa agagcacatt gctttgtttt gcctagttgg
     4141 cacgaaggaa tggcaaatac taaccttgaa tcagcagcat cagggagacc aattattaca
     4201 agtgatatcc cagggtgtaa agaagcagtt attgaatcga agtctggtta tttatgtgaa
     4261 agaaaaaata gttgtagtct ctataattcg atgaataaat ttctaaaatt atcatatgag
     4321 cagcgcatta acatgggaaa aatggggaga aattatatga ttaaaaaatt taataagaag
     4381 aaagttgtcg atgaaacaat taaatcatta tttagttaaa aagatatgtt ttgcaaagta
     4441 tgtgatagaa aagagggatt ctgtggcttc agaatagata tcaaagaatt ggcaaaagtt
     4501 tctgatattt ttttatttac tacattacaa gaaggaatgc caagatctat gatggaagct
     4561 atggcaagtg gattgccatg tatagcaagt aaaatccgag gtaatgtaga ccttttagtg
     4621 gaaggaaaag gtggatatct tgttccagct aaagatgcag aattagtagc agagtgtttg
     4681 agagaactaa tcaataataa atctcttaga aagtatatgg gaaatgagaa cctttccaga
     4741 attaaagaat tcgaggttag tatcgtaaaa aaaagaatca aagaaatata tgaagaagtt
     4801 ataggtgaga gtgtaaaatg aaaaatccaa tgttttcaat aatagtacct atctattgcg
     4861 ttgaaaaata tttgaaagat tgcttagatt caataagaaa tcagacattt aaaaattttg
     4921 aagcaatact agttgatgac ggtagtcctg acgaatgccc agaaatatgt gactattatt
     4981 cccaaataga tggacgttta aaagtaattc ataagaaaaa cggtggaata atagatgcac
     5041 ggaaaacagg agtcactagt gctatgggag agtatatagt ttttctagat ggtgatgatt
     5101 ggcttgcaaa tacttattta gaaaaatatg cggaagtaat tactaagtac aatccagata
     5161 taatatgtag tgggtatttt gcatcacgtt ccaacaaaat tagaaaaaga gaattgtctt
     5221 gctcgtttgg atattatgac aaagataaaa ttataaattt gatttatcct aaattaattg
     5281 aggatgaaca tggagagtat tttagaccac aattatgggc aaaagctttt agaactactc
     5341 tgtacaagga acaacaacaa gtatctgggc aagttagtgt tggtgaagat catgcatgta
     5401 caaaaccatg tatttaccat gcaaattcga tatatatgat ggaaaaatgt ttatattatt
     5461 acagaataaa cgaaaattct ataacaaaaa aaccacaacc atattattgg gaaggaccaa
     5521 aggctattgg agagcatttt gaaaagcaga ttgatatgaa taagtatgat tttcaagatc
     5581 aagtatatag aaatgttaca cacaatttat ttaatgttgc tgtttcacaa tttaataaac
     5641 aggacaaata tttaaatata aaaaaagata ttatacaaaa tctaagtgac gaatactata
     5701 ctagagctat ttgtaaatgc aaatataaag ataattggaa aggaattagt gctaagtttg
     5761 cattgaaaaa caaatgggta tttttaattt atatttataa caaattgata aaga
//
LOCUS       DAYYCQ010000127         5771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000127 DAYYCQ010000000
VERSION     DAYYCQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.130"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /locus_tag="ACHAYF_002746"
     CDS             complement(<1..302)
                     /locus_tag="ACHAYF_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8580100.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
     gene            395..744
                     /locus_tag="ACHAYF_002747"
                     /pseudo
     CDS             395..744
                     /locus_tag="ACHAYF_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1016..1306)
                     /locus_tag="ACHAYF_002748"
     CDS             complement(1016..1306)
                     /locus_tag="ACHAYF_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580101.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1708..1944
                     /locus_tag="ACHAYF_002749"
     CDS             1708..1944
                     /locus_tag="ACHAYF_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580102.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="ACHAYF_002750"
     CDS             1951..3903
                     /locus_tag="ACHAYF_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8580103.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="ACHAYF_002751"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="ACHAYF_002751"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8580104.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4799..5491)
                     /locus_tag="ACHAYF_002752"
     CDS             complement(4799..5491)
                     /locus_tag="ACHAYF_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8580105.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1895 a          997 c          917 g         1962 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagttgaa
     4561 taagcaattt tttctataag ttatattatc ataataccac aaaatcacaa attttcattc
     4621 aattatcatt ttcaattgca ttttataaat cattttgtat gtaatttggt atgtaagtta
     4681 aaatgaaaaa agtagtccat tttgaagtac attttgtcat tttgtactcc aaaactgaac
     4741 tacttagtat agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt
     4801 atttacgcaa atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat
     4861 cttttgcttt ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt
     4921 cgtctaattt cttttgtgta tctgttaact gagtttctac atttgttttt tcttgtgtca
     4981 gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt
     5041 gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt
     5101 ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct
     5161 tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt
     5221 ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct
     5281 gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta tctgtcaatg
     5341 tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca
     5401 aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga
     5461 caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta
     5521 catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta catatgtaat
     5581 tttaatataa atactatcaa atactcattt aatcctcctt ttttatctat ttaaaaaatc
     5641 ttttttatag agaaaaatgc aggttaatac tttcttaaat aaatatttat ggtgaacaaa
     5701 aacaaaaaag caagttttat taaacgtaac ccttttcatt tcatagatta ctgtatttat
     5761 tacaaaaaac g
//
LOCUS       DAYYCQ010000128         5705 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000128 DAYYCQ010000000
VERSION     DAYYCQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.131"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..493
                     /locus_tag="ACHAYF_002753"
     CDS             <1..493
                     /locus_tag="ACHAYF_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFM8580106.1"
                     /translation="KKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDAIS
                     IYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYN
                     LKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERI
                     LTI"
     gene            774..1994
                     /locus_tag="ACHAYF_002754"
     CDS             774..1994
                     /locus_tag="ACHAYF_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8580107.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1991..4711
                     /locus_tag="ACHAYF_002755"
     CDS             1991..4711
                     /locus_tag="ACHAYF_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8580108.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4695..5639
                     /locus_tag="ACHAYF_002756"
     CDS             4695..5639
                     /locus_tag="ACHAYF_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8580109.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2121 a          911 c         1122 g         1551 t
ORIGIN      
        1 gaaaaaaatt tttcacgagg tagggagtga attttctatc aaagagctaa actatgagct
       61 tgagtatgaa aaatctattt gttcaatccc ttataataat attgaggaaa ttatagaaaa
      121 ggatgctatc agcatttaca aattttttat ttttattcct aatcgtcaat tgaaagctaa
      181 tatatttaac catttgaaaa agatgctaaa tgtgtctgtt actgaatcgg cacctgtaaa
      241 tattgaaatc gtacctcaaa atgtatctaa aggtgtggtg tttgatcgtt tggaatcaat
      301 ctacaattta aagaaacctt tgagaatcgc gatcggagat agtttaaatg acttatctat
      361 gtttgaaagt gcagatatta gttttgcaat gggaaatagt catcaagaaa tcatgaaaaa
      421 agctgattat gtcactgttt caaatgataa agacggagta tctcaagcat ttgaaagaat
      481 tcttaccata taaactaatt aaggtagtat agaacataga attctaaata cggaaaaatg
      541 gagcataaat tccagtacaa atagtacttc ttcagtgtca aacgaagcga atataacata
      601 gatatttagg attgtgacaa aaatcatgtc acaatctttt tttcgaataa acttattttt
      661 tgaagtcggc catttatcgt aatttctttg cataatacga acaaattttc ggttattgat
      721 gaaaaaaaac attgaaaatg gtatgataaa agaaaaaaag gaggaaattt ttaatggtga
      781 aatttatcca tgcggcagat ttacatatgg atcggtcatt tgaaggactg acaactcttg
      841 ataaaactgt acaggaaaaa ctattgaaga cgaatctgac cgttttatca aatatcattg
      901 aacaggcaat aataaataac gttgattttg ttttacttgc aggagataat tttcatcaaa
      961 atcgtccttc attaaaaata cagaaacatt tttcagaaca gatgaaacgg ttagaaaaga
     1021 accatatacc tgtatttata atattcggaa atcatgattt ctatcaaaaa gagcggtatt
     1081 ggttctcctt tcctaaaaat gtccatttat ttacatccga aacggtagag acaaaaaaaa
     1141 taacgataaa aagtggagaa actgtctctc tcagtggatt tagttatcgt caaccgtgga
     1201 ttcaaaaaga caaagtgatg gaatttcctt ctagagaact cacagattac catattgggt
     1261 tgtaccacgg ggaacctggc gtctctcaaa aaggaaacta tgctcctttc caaccctcaa
     1321 agatgcaaga aaagggatac gattattggg ctttagggca tatccatgtg ccaacggtct
     1381 taaatgaaaa gcaaacaatt gtttatccag gtgcgcctca aggtcatact aaaaaggaac
     1441 aagcttctac atctattcta ctagttgaat taagtaaagg aagttgccaa atacatccga
     1501 ttaaagtagc agaggtctat tggaagacaa aggaaatatc attgaggaaa gcaagaacga
     1561 caaaagaggt cattgttcac attcgtcagc agctttcaaa agtagaggat ggatttagtt
     1621 taattgagat aaaattaaaa gattatgacc atctcaatac agatgttttg gaacggatac
     1681 aatcaggaga gctattagac tatttgttag aggaattctc agataggata aatgatttct
     1741 ttatttggcg tatttctctc atagagaata ctttttatac gaagacccca cttgctgctt
     1801 ctgctaaatt gatggaacag ttatttcaat actatcagac acctcaagat ttccaacaaa
     1861 ttttaaatga agtatatagt catcaagaag cggctagaat attgagcgaa ctgccggaat
     1921 atcaagaaga aacacttgaa aaagccaagc agctgcttaa tcaagatttt ttatttgagg
     1981 aggatcagga atgaagattt taaggatcga gattgctgct tttggaaaat ggcgtcaaaa
     2041 aagttttgac ttttattcag gcaatcagct gatctatggg ggaaacgaag caggaaaatc
     2101 aacgatttat caatttattc aagcaatatt atttggtttc ccttcaaaag gccgtaaaaa
     2161 aaaggattat acaccaaaag acggctcagc ctatggcggc aaaatatggc ttaaacatcc
     2221 tgtatatggt gaatttgctg tcgagcgcta caagcagcaa aatcgcggaa aatcgaaggt
     2281 gtggctgggg gatcaagtgg gaagtgatga gttactagaa aaactgatcg cacctttgac
     2341 gaaagagtta ttccagcagg tatttacttt ccagcaagag cagttgatgg aactagatca
     2401 tttgagagaa aaagagctac atgatgcgct gatttcttta ggcatcacgg gcagttctca
     2461 agtattccaa aagcggcaag aatattatca gaaagcacaa caattattca agaaaaaagg
     2521 ccaaaaatta ctaatcaatc aaaaattaaa tgaatggaaa gagctgcaag aaaaaattca
     2581 tcaaaaacaa gaacaggaag cttattttgg caagttgatc caacaagaac aggaatacga
     2641 gtcgcaacag cagcagttat tggcacaatt aaaagaggcg aacaaacaat tttcattagc
     2701 cagacagcaa gaacttaatt tttctttata tgaagaatgg caatctctca aaaaaaacag
     2761 cactacaggt cttcctgatg agcaacttct tttagatctg gagtctttct ccaaacggta
     2821 tcagcaaaca aatgagcgtt tggaagaatt agaagcaaca ctggaaaagc atagcgggat
     2881 ggatcagcag tcagccagat attattttta tttagagcag gagcaggaac taaaagatct
     2941 ccagcaactt caatttacag cggaacaatt aaacgaagaa tggcaaagat tacaaattga
     3001 gactagagaa acaaattcag aattgaagat tttggaacaa aggtggcatt ggcagaaaaa
     3061 tccgttaccg gaagaatttt tatatgaaga agaatggaag catcttctgg ctgaagaaag
     3121 aaggattgct gatctagaaa gccagatttc tgtgcgtctc caaatagcta aagaacagca
     3181 agagatgatt gaagcagaag tgaatgactt ggaagagacc tatccagcat tattacaaaa
     3241 tcagtcagag atagcaccag tctcttcatc taaaccgtat atgctgatgg ctggcggagc
     3301 aataatcatt gcaggtatat ttgtaccaat gcccttgaaa gtgatccttt tactaattgg
     3361 tgcaggcatg attggctatt tcttctacaa aaggcaacca aaaattaaaa ctaaaaatga
     3421 tttctctgag ataaaaggaa tgtggcaaga aaaactagga caacttgatc tagccttagc
     3481 tcaattagct gaagcagaag aaaaacaaaa gagaactgga gaagaaaaac agattcttca
     3541 gcaagaagta tatcgttttg cggatcatcg gcacttagga aagatggata cgctcaaact
     3601 catgaaagaa tatggacagg aagtcataga ttatcagaaa ttatatggcg tatttgagcg
     3661 gaaacataaa agagaacagg aaatacaaga aaaactacga caaatcgacc aaaaatttga
     3721 gtttgttaaa gaatggttac cgatacaaga taagacaatt tcagaaaaaa tggtattgct
     3781 ttcccgtttt atacaagaga tggaacagat gaaatttgcc agaagctatc agcaaaatac
     3841 tttattgaag caagaaatca atcaattgaa aaagaaacaa aaagtatgga tacaagaata
     3901 cagcgaacaa ttgactcaag caggcttatc ttatccttcg gaagtacctg tctttttaca
     3961 aaaagccaaa caagccgaac agcagatgaa aagatacaat gaattatcgg aaatgcttac
     4021 tccactattt cctgaagagc tgacaaaaga gaaattatct gtaagactgc gccaactgaa
     4081 agaaaagcaa tcccaactcc agcaagaaga aaaagaagca agtgaaaatc ggcaacgact
     4141 ccaacttcaa gtagaagagt tgcaaagaga cggcacattg gacgaacttt atcaacaagc
     4201 tagccggcaa aaagcaaaat tacaggaatt actggaggaa tggggagcac ttgaaatcgc
     4261 cgggacattc ttaggtgatc tttcaacaga gatgagtgaa agacaattac cccagttgtt
     4321 aagagcagca ggtgattatt taggtatttt aacaaaagga aattatcaga agatattact
     4381 gaaaaatgat acactgactt taacagacgg atatacagaa tttcccatct atgagttatc
     4441 tacaggtacg aaagatcagc tgattatggc gatgagattt gcttacctct cattagaagc
     4501 taaccgttcg atatgcccga tcatcattga tgatggctgg cttcattatg atcatcagcg
     4561 aaagtatcaa ttagctgaac ttttagctaa atttggagaa aagtatcaaa taatttgttt
     4621 ttcttctgat aaagaaatgg taagctatta ccatgagatg aaccaaatcg taaaacaatt
     4681 agaaggagca aataatgaaa aaaattcgtg atttagtaat cgatgagcag tttgaacaat
     4741 ttgtcctgat caaaagtgca gatgtaagaa tcgctaaaaa cggaaaaaaa tttattgctt
     4801 ttactttcca agatacttca ggtactatcg atggtaaata ctgggatgct tcggaggagg
     4861 aaatcaataa atttacagcc ggcacagtcg tttttctaaa cggtaaaaga gaggtttatc
     4921 aaggaaaccc tcaagtaaag attcttcata tgcgcttaac aagggtagat gagccaggag
     4981 agcctagttt atacatggaa agagcccctt tgaagaaaga agaaatggaa gaagaaatca
     5041 atcaattgtt gtttgagatc accaatgcgc attggaatcg tattgttcgc tatctactta
     5101 ataaatatca aaaacaattt tttgagtttc cggcagcaaa aagaaaccat catgcatttg
     5161 ccagtggatt agcttatcat actttgacaa tgcttcactt agggaaatca atcgccgatg
     5221 aatatcctga gctgaatcga gcattgttgt atgctggtat cattttgcat gatttaggaa
     5281 aggtgattga actttccggt cctatgtcga cagaatatac gcttgcagga aatttgatcg
     5341 gtcatctggt attgattgac gaagaaataa ctaaagcctg tcttgcgttg aaaatcagcg
     5401 aagctgacga agatgttctc gtattacgtc atatggtttt agcacaccat ggcttattag
     5461 aatatggctc acctgtacgt ccaagaataa tggaagcaga aatattacat caaatcgaca
     5521 atatcgatgc ttcgatgcag atgatgttga cttcgattcg tcaaacagaa ccaggaaaat
     5581 acaccgatcg gattttcggt atggacaacc gaagtttcta tgtaccaaaa gatttatagc
     5641 aaaaatttat ttttttcatt caaaacaaaa aaagcgagac ataagtcttc gctttttttg
     5701 ttttc
//
LOCUS       DAYYCQ010000129         5594 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000129 DAYYCQ010000000
VERSION     DAYYCQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.132"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..862
                     /locus_tag="ACHAYF_002757"
     CDS             5..862
                     /locus_tag="ACHAYF_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8580110.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            884..1393
                     /locus_tag="ACHAYF_002758"
     CDS             884..1393
                     /locus_tag="ACHAYF_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8580111.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1430..1831
                     /locus_tag="ACHAYF_002759"
     CDS             1430..1831
                     /locus_tag="ACHAYF_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580112.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1943..2296
                     /locus_tag="ACHAYF_002760"
     CDS             1943..2296
                     /locus_tag="ACHAYF_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580113.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2330..2773
                     /locus_tag="ACHAYF_002761"
     CDS             2330..2773
                     /locus_tag="ACHAYF_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8580114.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2790..3875
                     /gene="uxuA"
                     /locus_tag="ACHAYF_002762"
     CDS             2790..3875
                     /gene="uxuA"
                     /locus_tag="ACHAYF_002762"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8580115.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3868..4581
                     /locus_tag="ACHAYF_002763"
     CDS             3868..4581
                     /locus_tag="ACHAYF_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8580116.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4788..4937)
                     /locus_tag="ACHAYF_002764"
     CDS             complement(4788..4937)
                     /locus_tag="ACHAYF_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580117.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            5333..5533
                     /locus_tag="ACHAYF_002765"
     CDS             5333..5533
                     /locus_tag="ACHAYF_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8580118.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1941 a          856 c         1215 g         1582 t
ORIGIN      
        1 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
       61 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
      121 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
      181 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
      241 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
      301 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
      361 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
      421 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
      481 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
      541 cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga
      601 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
      661 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
      721 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
      781 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
      841 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
      901 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
      961 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
     1021 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
     1081 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
     1141 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
     1201 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
     1261 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
     1321 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
     1381 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
     1441 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
     1501 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
     1561 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
     1621 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
     1681 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
     1741 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
     1801 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
     1861 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
     1921 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
     1981 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
     2041 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
     2101 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
     2161 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
     2221 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
     2281 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
     2341 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
     2401 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
     2461 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
     2521 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
     2581 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
     2641 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
     2701 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
     2761 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
     2821 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
     2881 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
     2941 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
     3001 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
     3061 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
     3121 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
     3181 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
     3241 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
     3301 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
     3361 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
     3421 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
     3481 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
     3541 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
     3601 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
     3661 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
     3721 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
     3781 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
     3841 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
     3901 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
     3961 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
     4021 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
     4081 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
     4141 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
     4201 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
     4261 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
     4321 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
     4381 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
     4441 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
     4501 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
     4561 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
     4621 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
     4681 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
     4741 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
     4801 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
     4861 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
     4921 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
     4981 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
     5041 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
     5101 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
     5161 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
     5221 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
     5281 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
     5341 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
     5401 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
     5461 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
     5521 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt
     5581 tgtacgaaaa aatt
//
LOCUS       DAYYCQ010000130         5339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000130 DAYYCQ010000000
VERSION     DAYYCQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.133"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHAYF_002766"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHAYF_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHAYF_002767"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHAYF_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACHAYF_002768"
     CDS             2090..3130
                     /locus_tag="ACHAYF_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8580119.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3954..4286
                     /gene="traE"
                     /locus_tag="ACHAYF_002769"
     CDS             3954..4286
                     /gene="traE"
                     /locus_tag="ACHAYF_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8580120.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4905..5246
                     /locus_tag="ACHAYF_002770"
     CDS             4905..5246
                     /locus_tag="ACHAYF_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8580121.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1769 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     3901 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
     3961 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
     4021 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
     4081 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
     4141 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
     4201 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     4261 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     4321 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     4381 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     4441 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     4501 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     4561 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     4621 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     4681 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     4741 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     4801 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattgata
     4861 taaaacatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     4921 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     4981 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     5041 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     5101 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     5161 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     5221 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     5281 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaat
//
LOCUS       DAYYCQ010000131         5333 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000131 DAYYCQ010000000
VERSION     DAYYCQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.134"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..388
                     /locus_tag="ACHAYF_002771"
     CDS             32..388
                     /locus_tag="ACHAYF_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFM8580122.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="ACHAYF_002772"
     CDS             404..754
                     /locus_tag="ACHAYF_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFM8580123.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="ACHAYF_002773"
     CDS             783..1562
                     /locus_tag="ACHAYF_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFM8580124.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="ACHAYF_002774"
     CDS             1573..2235
                     /locus_tag="ACHAYF_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFM8580125.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="ACHAYF_002775"
     CDS             2254..3360
                     /locus_tag="ACHAYF_002775"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFM8580126.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="ACHAYF_002776"
     CDS             3350..4441
                     /locus_tag="ACHAYF_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFM8580127.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="ACHAYF_002777"
     CDS             4454..5194
                     /locus_tag="ACHAYF_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFM8580128.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1718 a          870 c         1267 g         1478 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca ata
//
LOCUS       DAYYCQ010000132         5312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000132 DAYYCQ010000000
VERSION     DAYYCQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.135"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHAYF_002778"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHAYF_002778"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8580129.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHRELGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHAYF_002779"
     CDS             complement(1297..2133)
                     /locus_tag="ACHAYF_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8580130.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHAYF_002780"
     CDS             complement(2317..3534)
                     /locus_tag="ACHAYF_002780"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8580131.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHAYF_002781"
     CDS             complement(3683..3844)
                     /locus_tag="ACHAYF_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580132.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHAYF_002782"
     tRNA            3917..4000
                     /locus_tag="ACHAYF_002782"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5312)
                     /locus_tag="ACHAYF_002783"
                     /pseudo
     CDS             complement(4240..5312)
                     /locus_tag="ACHAYF_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1655 a         1082 c          837 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctag
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAYYCQ010000133         5130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000133 DAYYCQ010000000
VERSION     DAYYCQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.136"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1010
                     /locus_tag="ACHAYF_002784"
     CDS             <1..1010
                     /locus_tag="ACHAYF_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8580133.1"
                     /translation="VEKYIDECINSLINQTLKDIEIILLNDGSTDNTLAILKKYEKLD
                     SRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFESLYNCAIAENADIA
                     IGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNRLYSHNAWDKIYRR
                     SLVLEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYRQQNNSIMHKPKSN
                     LVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQKNVGGTCEDYKNAL
                     VSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKMYKTAYNMLWRIYN
                     R"
     gene            1024..2103
                     /locus_tag="ACHAYF_002785"
     CDS             1024..2103
                     /locus_tag="ACHAYF_002785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8580134.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIY
                     GEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEAL
                     AIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQK
                     GIKLRKELTSKNISNIWYEYLNQFF"
     gene            2122..3546
                     /gene="wzy"
                     /locus_tag="ACHAYF_002786"
     CDS             2122..3546
                     /gene="wzy"
                     /locus_tag="ACHAYF_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFM8580135.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIG
                     IYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNG
                     IYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNG
                     GYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIV
                     KNFVYGVLIILLINFFLQQFNKRL"
     gene            3592..5097
                     /locus_tag="ACHAYF_002787"
     CDS             3592..5097
                     /locus_tag="ACHAYF_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8580136.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIILVNVLFYRRYVVKIISKIIVKIKKI"
BASE COUNT         1831 a          606 c          922 g         1771 t
ORIGIN      
        1 atgttgaaaa atatattgat gagtgtataa atagtttgat aaatcaaaca ttgaaagata
       61 ttgagattat tttgttaaac gatggttcaa cagataatac gttggcgata ttaaagaaat
      121 atgaaaagct tgattctaga ataaaagttt acgaacataa aaatatgggg ttagggccaa
      181 ctagaaatcg agggatcaaa atagctaatg gagaattttt ggcttttgtg gattctgatg
      241 attttgtttc caatactatg tttgaaagtt tatataattg tgcaattgca gagaatgcgg
      301 acattgcaat tggagaagta ggcatttttt ctgatagaga aatagaaaga gtaaaaataa
      361 gaaaaagtct aacgaactta aaaacaattg aattagcaac atataacaag aaagaatttt
      421 ttaagcaatt ctatttcaat cgtttatatt cacataatgc ttgggataag atatatagac
      481 gtagtttagt attagagttt aatttatcat ttggtgataa caaaataatt tttgcagaag
      541 ataattggtt tcaattacaa gtatttatga caaatacaag aatctgtttc gtagataaat
      601 tgtgttatta ttatcgccaa caaaataatt ctataatgca taagccaaaa agtaatttgg
      661 tttttagaca tggaaatatg attgctgatt atgaaaagtt gataaggcaa aatggaaaca
      721 acataataga taaaaaagct tgtatgttgg ttgcatttga tgtactaatt atggagattt
      781 ttaatcaaaa aaacgttggt ggaacatgtg aagattataa gaatgcatta gtttctttga
      841 aacatcataa aatattgaga gaaaatattt caaatatagt tagaatgaaa gcatatgaac
      901 ttgaacctaa tatgagaaag aagttagccc ttgtgaccat tagtataatg tttaatttta
      961 aaatgtataa aacagcgtat aatatgctct ggagaatata taatcgataa agaggtagga
     1021 aatatgagga aattgacctt tgtgatagga attatgggaa aaggaggggc agaacgagta
     1081 attgcgaatt tggcaaataa ttttattcaa gataactggg aaattgatat tataactata
     1141 tatgggaatc ggcaagacta tgaactgaat agaaaaatac aggtacatcc aataatatgt
     1201 aaaagttcgt ttcgtgcatt tagacctttg gaacgcttta aggaacttag gaaaaaaata
     1261 agggaaatac agcctgatct ggttatttcg ttccttgctg atgtaaatat ccatgtcatt
     1321 atttcattgt taggagtaaa acaaaaatta attgtttctg aacgaaatga tccacaacgg
     1381 aatccagaag aaccatggat taggaaaatt agagattttc tttataaaaa tgtagatgga
     1441 attgtttttc aaacaccaga cgcaaaaaaa tattttagta agatcaatga taaaaaagta
     1501 aaaacaatta taattcccaa cccaataaag gaaaatttac cttattataa taaaactaca
     1561 agtagtaaaa aatttattac tgcatgtaga ttggataaac aaaaaaattt gcccttaatg
     1621 attgatgcaa taggaaaact tattcatgaa ggatatgagt gtgaactgga tatttatgga
     1681 gaagggggat taaaaaccga gcttgaaagt tatatcaacc ataatggatt aacagaattt
     1741 gttcatctaa aagaatttac aaataaaata catgaaaaga tgcaaaatgc ggcaggattt
     1801 gtaatttctt ctgattatga aggtatatct aattctatgt tagaagcact ggctattgga
     1861 gtgccagtta tcgctacaga ttgtccagta ggtggcgcaa gaatgtattt aggtgataga
     1921 aatgaaatag gtcatcttgt tgaatgtaat aatctagata agttaacaga tgcaatgcgt
     1981 agggtacttt tggatagaga aagtgtggta agaaaagcgc aaaaaggaat taaactaaga
     2041 aaagaactga catctaaaaa tatttcaaat atatggtacg aatatttaaa ccagttcttt
     2101 taaccaaaag gaggatattg tgtgaaagaa aaaataaata tattgatgtt ttcgttaacg
     2161 ttagtcgcgt ttacaatatt ttgcgctttt tcattaaatc agaactattg gctaaattgg
     2221 cttggaatat tgatttactt atatgcaatc tgtacatggc attggagaaa aggagattca
     2281 atattttcat tatacacgat ttttttcact ctttttttat tatttagtta tgggcaatgc
     2341 attatgtggg cattcggtat tggaacagat cgtggaatcg gtacaacagt agtagcttat
     2401 ggtacgggga ttattcctag tgaatcagat atgataatgg taaaatggta ttcttgtatt
     2461 tctatgtttg tatttcacat aggagcactc ttatttacga gaaaaaccac actaagaaaa
     2521 gttagttggt atgaatctgg agatgctgat gccgatagaa agtttttatt taaaataggg
     2581 tgtatcattt ctataatagt agtaccgata gtttttgttt ctaaattact agaggtacgt
     2641 atagcattag tccatggtta taatgcatta tattatggtg attatgctac acaaagtgga
     2701 tatttacaaa ttattctata tctttttttt ccagccttaa tatgtttatt aattggtagc
     2761 aacttttcaa aagtaataac gagatttgtt tttattattt ttgctttata ttctttatta
     2821 ggaattatgt caggagacag aggaagttgg ttgtatagct ctattatact catatgggca
     2881 tacactcaat ataaaggaac ctttaattat aaaaaattaa taaagtggct tattttagct
     2941 gttataggaa tatatattct aaacgtaata acgaaagcac gtgatggtgg aggactaagt
     3001 aaacttactt taaaagattt tacatctgta tttgattcag atgattctcc tttagttgat
     3061 tctttttttg aaatgggggg gactatgagt attataactt ttttcctaat aagtggtaat
     3121 ggaatatatc cctttgctaa tacttatttg acctcactct taggctctat atcaacaaga
     3181 atgctatcaa tgtttggaat aaagtttgtt ttattagctg attggttttc tcaggattat
     3241 ttaggaatca cttgggggac tggattctca atgattgcag aagcctatgt caatggtgga
     3301 tacattgggg gtatagtcta tatttttatt attggtatag tcatcggtaa aatcctttct
     3361 aatagtatag acaaccaaga tattgaaaat catcctatga agatgttttt ggctataact
     3421 acagcaaata ttataatggg ttttcctaga gctgcttcat atttaattgt gaaaaatttt
     3481 gtgtatggtg ttttaattat attgttgata aactttttcc tacagcaatt taataaacgt
     3541 ctttaatatt taagcttaat aaaaagataa aacaagtcag gagttgcaaa aatggaaaga
     3601 actgctcggc tcaaaaaaaa cactatttgg gctctggtgt ttcaaataac ttctattgtc
     3661 tgtggtatga tcttgctacg tttgatatta acaaagtacg gatccgaggt taatggaatg
     3721 attagctcta ttaaccaatt tctacaaatt atttcttttt tacaattagg agtaggcgct
     3781 gtaatgcaat caactctata caaaccacta gcagaaaagg ataatttaat gataagtcaa
     3841 atcttagctt ctggagggaa gttttatcgg caaattgcat atattctact agtatacgtg
     3901 ggtttgttac ttgcagtcta ttatgttctc gttcctaatg aatttgggag atcatataca
     3961 atggcaatga ttgcaacatt aagtattaat tcatttgctc aatattattt tggtattgtt
     4021 aatactttgt tattaagtgc tgaccagaaa atttatatta cgtatattgc acagacaata
     4081 gcatttattt ctaatacagt tgcatgtgct ttattaatta gtctgaacac agagatcctt
     4141 atggtaaaac ttattactgc tataatttat cttgcttgtc caatatatta tagatggtat
     4201 gtggatcgaa actataatat aaagaggaat gtgtcatatg ataaggaacc gatccctcaa
     4261 aaatggaatg gggttgcaca acacgtagct tctgttgtac tagatagtac tgatgttgta
     4321 gtgttatcta tattttctaa cctgatatat gtatctatat ataatgtgta caatttagta
     4381 atatacggag taaaacagct attcatggct atgacaaatg gcgttcaggc cttactaggg
     4441 gaactttggg tcagaaaaga aatagaagtt ctttacaaaa catttgcatg gttcgaatgg
     4501 attgtacata ctttgacagt ttttattttt ggctgtaccg gtatactaat cgttccattt
     4561 attcaagtat atacaagagg agtaacagat gctaattata atcaattatc attcgcagct
     4621 ttacttgtaa ctgctaacgc acttcactgt ctgcgtacac cttatcatgt aatgataaaa
     4681 gcagctggta aatataaaga aactcaaaat agttttataa ttgcttcttt gttaaattta
     4741 ggtttatcat taattttggt taaaccacta gggctagttg gagtagcatt aggatctcta
     4801 atttcaatca catatcatac actttggagt gcaaattata attcaaaata tttgcttaaa
     4861 tggccgatga aaaaattcta taaacagtta tttgtaaaca tcttaactgt agttctttct
     4921 tttggggcga catcacaatt gaaaatgatg tcaatcacat atttggattg gattgttctc
     4981 gcaattaaat gtgcagttat tgtatttata tgtataatcc tagttaatgt tttattttat
     5041 agaagatatg tagtaaaaat aattagtaaa atcatcgtaa aaataaagaa aatttaatac
     5101 acaataaatg ttttaataaa cttaataaaa
//
LOCUS       DAYYCQ010000134         5126 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000134 DAYYCQ010000000
VERSION     DAYYCQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.137"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(253..1101)
                     /locus_tag="ACHAYF_002788"
     CDS             complement(253..1101)
                     /locus_tag="ACHAYF_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8580137.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1265..2167
                     /gene="gnd"
                     /locus_tag="ACHAYF_002789"
     CDS             1265..2167
                     /gene="gnd"
                     /locus_tag="ACHAYF_002789"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8580138.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2169..3170
                     /locus_tag="ACHAYF_002790"
     CDS             2169..3170
                     /locus_tag="ACHAYF_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8580139.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3167..3451
                     /locus_tag="ACHAYF_002791"
     CDS             3167..3451
                     /locus_tag="ACHAYF_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8580140.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3484..4281
                     /locus_tag="ACHAYF_002792"
     CDS             3484..4281
                     /locus_tag="ACHAYF_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8580141.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4295..>5126
                     /locus_tag="ACHAYF_002793"
     CDS             4295..>5126
                     /locus_tag="ACHAYF_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8580142.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
BASE COUNT         1651 a          839 c         1099 g         1537 t
ORIGIN      
        1 aaaataagaa acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta
       61 actttgttat cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat
      121 gcgatgacat acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca
      181 aaaaagccac taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt
      241 tctgttatgg tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg
      301 acgtgattct ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag
      361 ttgagaaata cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat
      421 aaaaacgaca tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt
      481 ttttgctcca gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt
      541 gtgagaaatc aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat
      601 atggtaatcc gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc
      661 agttgctaca atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat
      721 aatagtagcc gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt
      781 tgaaatacta gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat
      841 attttcgtgg atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa
      901 atctttaaat ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat
      961 gcccagttca cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa
     1021 aatataatcg gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg
     1081 tagtcttatg gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc
     1141 cgttctcatt aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt
     1201 tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc
     1261 tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt
     1321 aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc
     1381 aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca
     1441 gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa
     1501 actttttcca aaactcggac caggagacat tattattgaa ggcggaaact cttattataa
     1561 agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg
     1621 gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa
     1681 agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct
     1741 gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata
     1801 tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt
     1861 tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat
     1921 ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt
     1981 ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt
     2041 tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc
     2101 agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg
     2161 tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct
     2221 ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc
     2281 aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa
     2341 gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt
     2401 cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa
     2461 tcgtatctat catacctgct ttaattgaac gaaatgctgt agcagaaaat aatataacta
     2521 atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat
     2581 gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg
     2641 gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg
     2701 ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag
     2761 tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag
     2821 aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc
     2881 tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa
     2941 gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc
     3001 atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg
     3061 aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc
     3121 ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat
     3181 ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat
     3241 ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg
     3301 aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca
     3361 atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac
     3421 aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat
     3481 ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct
     3541 agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa
     3601 gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg
     3661 attgattccc ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat
     3721 gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca
     3781 caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa
     3841 gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct
     3901 gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct
     3961 atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa
     4021 gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag
     4081 ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt
     4141 ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg
     4201 catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa
     4261 ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag
     4321 tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac
     4381 gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga
     4441 agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc
     4501 tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc
     4561 attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct
     4621 cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga
     4681 caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac
     4741 ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc
     4801 attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt
     4861 agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa
     4921 agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga
     4981 tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc
     5041 gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga
     5101 cgaagatgac tatgatgacg gatttt
//
LOCUS       DAYYCQ010000135         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000135 DAYYCQ010000000
VERSION     DAYYCQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.138"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHAYF_002794"
     CDS             85..972
                     /locus_tag="ACHAYF_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8580143.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHAYF_002795"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHAYF_002795"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8580144.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHAYF_002796"
     CDS             2274..2720
                     /locus_tag="ACHAYF_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8580145.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHAYF_002797"
     CDS             2993..4345
                     /locus_tag="ACHAYF_002797"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8580146.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHAYF_002798"
     CDS             4516..4803
                     /locus_tag="ACHAYF_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8580147.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYCQ010000136         4879 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000136 DAYYCQ010000000
VERSION     DAYYCQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.139"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHAYF_002799"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHAYF_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8580148.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACHAYF_002800"
     CDS             complement(2066..3064)
                     /locus_tag="ACHAYF_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8580149.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACHAYF_002801"
     CDS             complement(3290..3943)
                     /locus_tag="ACHAYF_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8580150.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHAYF_002802"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHAYF_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8580151.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1439 a         1017 c          753 g         1670 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttc
//
LOCUS       DAYYCQ010000137         4786 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000137 DAYYCQ010000000
VERSION     DAYYCQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.141"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHAYF_002803"
     CDS             <1..813
                     /locus_tag="ACHAYF_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8580152.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHAYF_002804"
     CDS             786..1229
                     /locus_tag="ACHAYF_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580153.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2401..3369
                     /locus_tag="ACHAYF_002805"
     CDS             2401..3369
                     /locus_tag="ACHAYF_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8580154.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            3379..3849
                     /locus_tag="ACHAYF_002806"
     CDS             3379..3849
                     /locus_tag="ACHAYF_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8580155.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            3865..4035
                     /locus_tag="ACHAYF_002807"
     CDS             3865..4035
                     /locus_tag="ACHAYF_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580156.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            4445..>4786
                     /locus_tag="ACHAYF_002808"
     CDS             4445..>4786
                     /locus_tag="ACHAYF_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM8580157.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         1825 a          778 c          789 g         1394 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcgattat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agttttgtag agagtgacct tataggcact ctcacattac gaacgatacg
     1501 tccctcggac gtactgaaag ggtgggactt atgagcgaac taatgcaaaa tttggctagc
     1561 aaaaaagcga aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aaacagttta tttcagcttt gtcatggcgt gtgccgactt
     1681 ttttaaaagt ctaggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaagg
     1741 cgtttcaaat gtctaagtat ataaatatat aagaatagag ttaagagtgc ttaaatcgaa
     1801 ttttagaggg aattcagaca attttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acagttttag caaaggagcc ggaggcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcaactata gggagtaaga
     2041 ttcccagttt agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctgta tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacaga
     2341 cattttgtat ttcataacgt ctgtatctct tgtgcgagta agaaaggagc tattttaaat
     2401 atgtctaatg aaatcgtaaa gtatcatcat gaattaaata caattccgtt gcgaaaattt
     2461 acaccagtcg aaatgaattt gttcttctct atcgtatcaa gaatgcgtga tgtaggtgat
     2521 aaaaaagtac aatttacatt cgagcaatta aaagatttaa gcaattataa agcaactgca
     2581 aatgttcgat tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt
     2641 ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg ttttatttaa tcaatttaaa
     2701 atcgacggaa aagctgatat tccttttgct gaaatacaag ttcatgaaaa agccttaccc
     2761 ttacttaata atcttgaaga atgggtgcgc tattctctcc aacaattcaa tgaattagaa
     2821 agcagttatt caaaaacaat gtttcgatta ctaaaacaat acagaaccaa aggtttcgct
     2881 tatttttcaa aagaagactt tcatgaatta cttgatattc ctaaaagtta caaccaaggt
     2941 acgatcgatc aacgtgtgtt caatccaata cgacaagaac taaccgcaat ctttaaaggt
     3001 ttaacaatca aaaagaaata cggaaaaggc cgtgggaagc ctgtcattgg ctaccaattc
     3061 acatttaaac cagaaatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
     3121 gaacaaaatc gggctttaga actagctaat cgacaaactc aacacgaaaa agaaactact
     3181 tcattacaag aaagaattaa ggaacaaaag cgaaaattag aacttttaca acagttagaa
     3241 gagttagaaa aacaacaaaa aaatagagaa aacgaaacgg ataaacaaaa gttagacaac
     3301 aattccgata ttcctctaga actaagaaaa gacttacttg ataatcttca aaacttattt
     3361 aaagactaag gagtattcat gacagataaa tcaataaaag aatttgcaga agaactaggt
     3421 gtcagcaaac aagccattag aaagtatttt gataaactgc caaccaagtt gacacctaag
     3481 ttgataggtg gaaaatacgt aatatcagca aaagctcaag agtttataaa aaatagtatc
     3541 aaccaaataa atgaagttga tactgaagtt gacagtttag ttgatagtat tttaattgca
     3601 gaattaaaag aaaaaaacaa acaaattgat catttacaag gggtcataga aatacaacaa
     3661 aaattactcg accagcaaca acaactcacc ttgcaggcaa atcaacaaat cgacaaacta
     3721 caaaaacggt tacaattagt ttatacggaa gaaacttcag aaaatgaatc aactactctt
     3781 tctgaagaaa cagaatcgat agaaaaacaa acaaagaaga agcaaaaatg gtggaaacta
     3841 tggaaataac tggaggaaac attcatgaca agaaaaaagc tcatatttgg ttctatttta
     3901 ctaggaattc taggcttcag tccgatgtta ttgtcagtga tcaataaagg aaataaacat
     3961 ggtgaattga aattgaatcc aaattattac aacagtccta acgaagagtt atttgtaaaa
     4021 gaaacggata aataataaaa aaagactgct ctaacgagca gcccaaaatg agattatcct
     4081 agtttagcaa cataaaccat ttattagatg aaaatgagat acaaacaaag cgtatcaaat
     4141 cctctcaaaa ataacaagtc tttaaaaaat gacagataaa aatgcacaaa gaattagata
     4201 tagataaccg cgaaaaatca agccttaaaa agagcttttt acctagacca atccaaaatc
     4261 acaaagtgag cctctgtatc ggttgataca agggctactt ggcatagtac acattatgtt
     4321 aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa
     4381 atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa
     4441 aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat
     4501 gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa
     4561 tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca
     4621 ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct
     4681 tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc
     4741 agtattttca gacgctgata atacgattaa atatatttat accgtc
//
LOCUS       DAYYCQ010000138         4740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000138 DAYYCQ010000000
VERSION     DAYYCQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.142"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..959
                     /locus_tag="ACHAYF_002809"
     CDS             153..959
                     /locus_tag="ACHAYF_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8580158.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            977..1804
                     /gene="vanW-B"
                     /locus_tag="ACHAYF_002810"
     CDS             977..1804
                     /gene="vanW-B"
                     /locus_tag="ACHAYF_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFM8580159.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1801..2772
                     /locus_tag="ACHAYF_002811"
     CDS             1801..2772
                     /locus_tag="ACHAYF_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8580160.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2765..3793
                     /gene="vanB"
                     /locus_tag="ACHAYF_002812"
     CDS             2765..3793
                     /gene="vanB"
                     /locus_tag="ACHAYF_002812"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8580161.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3799..4407
                     /locus_tag="ACHAYF_002813"
     CDS             3799..4407
                     /locus_tag="ACHAYF_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8580162.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4473..4646)
                     /locus_tag="ACHAYF_002814"
     CDS             complement(4473..4646)
                     /locus_tag="ACHAYF_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580163.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1332 a         1002 c         1262 g         1144 t
ORIGIN      
        1 attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga aaactacaga
       61 ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca gccacaaatc
      121 gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat cattccaatg
      181 cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt ctatgtgcgt
      241 tcattatttc gttcacagtc tgcacgctgt ttttggggtg gagactggct tccgtattgg
      301 aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact gacgtagtgg
      361 agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa caggaatgga
      421 gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg gagcttgagc
      481 aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa gatttgtttg
      541 atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga acaacagaaa
      601 aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc tacacctctg
      661 cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc gagcatcagc
      721 ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac gaggtttata
      781 gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca ccagacaaga
      841 cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc atcgaagctg
      901 ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca gaaaaatgag
      961 aaaaggatgt tatgttatgg acagaaaaag attgacacag cgtttcccgt tcctgcttcc
     1021 aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga atgagatttg acggctgtcg
     1081 ctatgcgcag acgatagggg aaaaatcgct ttcccatttg ctctttgaaa cggattgcgc
     1141 attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg tgttcaactt
     1201 aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa ccttttcttt
     1261 ctggtggctg gtacgccatg cggacaaaga taccccctat aaagacggcc ttacggtgac
     1321 caatggtaaa ctcaccacca tgtcgggcgg cggtatgtgc cagatgagca atttactatt
     1381 ttggatgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg aagtaaagga
     1441 gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattt cagagggctg
     1501 gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatggg tgaccttaga
     1561 tgatgagaaa atcatcggtc aggtgttcgc cgacaaagag ccccaagcat tatacaaaat
     1621 tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat atgcccaggt
     1681 taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc tgctttatac
     1741 aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagg aggagaacca
     1801 atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac aggatgaggc aaatgctttc
     1861 cgcgctttat cgccggattt tcatattatc cctacgctga ttagcgatgc gatatcggca
     1921 gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg taagccataa gtccgaggtt
     1981 tccgaggcga caattcttgc gctgagaaag gtcggggtaa aatacatttc tacccgcagc
     2041 atcggctgcg atcacattga tacgactgcc gccgagagaa tgggaatctc ggttggcacg
     2101 gttgcgtatt cgccggacag cgttgcggat tatgctttga tgctgatgct gatggccata
     2161 cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag atttcagatt ggatcgtatc
     2221 cgggggaaag aactggggga tatgactgtg ggagttattg gaaccggcca tatcgggcaa
     2281 gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc tggcctatga taacagccga
     2341 aaaatggatg cagattatgt ccagcttgat gagcttctaa aaaacagcga tattgttacg
     2401 ctccatgtgc cgctttgtgc ggatacccgc catctgatcg gtcagaagca aattggagag
     2461 atgaagcaag gcgcattttt aatcaacacc gggcgcggag cgcttgtcga taccggggcg
     2521 ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg cactggatgt gttggagggc
     2581 gaggatcagt ttgtttatac cgattgctcg cagaaagtgc ttgaccatcc ctttttgtcg
     2641 cagcttttaa ggatgccgaa tgtgatcatc acaccccata cggcgtacta cactgagcgt
     2701 gtgctgcagg atactacaga aaaaacaatc aggaattgtc tcaattttga aaggagttta
     2761 cagcatgaat agaataaaag tcgcaatcat cttcggcggt tgctcggagg aacatgatgt
     2821 gtcggtaaaa gccgcaatag aaattgctgc gaacattgat acggaaaaat tcgatccgca
     2881 ctacatcgga attacaaaaa acggtgtatg gaagctatgc aagaagccat gtacggaatg
     2941 ggaagccgac agtctccccg ccatactctc cccggatagg aaaacgcatg ggctgcttgt
     3001 catgaaagaa agcgaatacg aaacacggcg tattgatgtg gctttcccgg ttttgcatgg
     3061 caaatgcggg gaggatggtg cgatacaggg gctgtttgta ttgtctggta tcccctatgt
     3121 gggctgtgat attcaaagct ccgcagcttg catggacaaa tcactggcct acattcttac
     3181 aaaaaatgcg ggcatcgccg ttcccgaatt tcaaatgatt gataaaggtg acaagccgga
     3241 ggcgggtgcg cttacctacc ctgtctttgt gaagccggca cggtcaggtt cgtcctttgg
     3301 cgtaaccaaa gtaaacggta cggaagaact taacgctgcg atagaagcgg caggacaata
     3361 tgatggaaaa atcttaattg agcaagcgat ttcgggctgt gaggtcgggt gtgcggtcat
     3421 ggggaacgag gatgatttga ttgtcggcga agtggatcaa atccggctga gccacggtat
     3481 cttccgcatc catcaggaaa acgagccgga aaaaggctca gaaaatgcga tgattacagt
     3541 tcccgcagac attccggtcg aggaacgaaa tcgggtgcag gaaacggcaa agaaagtata
     3601 tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt tttttgcagg aggatggcgg
     3661 catcgttcta aatgaggtca ataccctgcc cggttttaca tcgtacagcc gctacccacg
     3721 tatgatggcc gccgcaggaa tcacgcttcc tgcactgatt gacagcctga ttacattggc
     3781 gttaaagagg tgataagtat ggaaaatggt tttttgtttt tagatgaaat gttgcatggc
     3841 gtccgttggg atgccaagta cgccacatgg gataacttca caggaaaacc ggtggatggg
     3901 tatgaggtga atcgcatcat cggcacaaag gccgtggcgt ttgctctgcg cgaggcacaa
     3961 atccatgcgg ctgcgcttgg ctatggcttg cttttatggg atggatatcg gccaagaact
     4021 gcggtggact gcttcctgcg ttgggcagcg caaccggagg acaatctcac aaaagaaaaa
     4081 ttttacccca atatagagcg agccgagttg attacaaagg gttatgtggc ctcacaatcc
     4141 agccatagcc gtggaagcgc aattgatctt acgctctacc acctggatac aggggaactt
     4201 gtttcaatgg gaagtaactt cgattttatg gacgaacggt cgcatcatac agcaaaaggg
     4261 ataggggatg cagaggcaca aaatcgaaga tgcttgcgta aaatcatgga aagcagcgga
     4321 tttcagtctt atcgctttga atggtggcac tataagttga ttgatgagcc ataccccgat
     4381 acctatttta attttgccgt ttcataatga aagtattggt tttgtatcag attttggatt
     4441 tgataaaatc tatgctggtc atggacaatc ttttactgga aagtggttaa agcatctttt
     4501 gtaatacatt gcaaattata gtattcaatc tgcccagcgg cagaaaagaa aaaaaccgct
     4561 gttgggcaga cctttcccac gcccattttg tattttgctg gcggcggttt tgagaaatca
     4621 aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc cataggagga tgccgctatg
     4681 ctgttttgct attgccatgc tctaatccac ttccaccact caaaaaaagc cttgcccctc
//
LOCUS       DAYYCQ010000139         4635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000139 DAYYCQ010000000
VERSION     DAYYCQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.144"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..519
                     /locus_tag="ACHAYF_002815"
     CDS             175..519
                     /locus_tag="ACHAYF_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8580164.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ACHAYF_002816"
     CDS             520..939
                     /locus_tag="ACHAYF_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8580165.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ACHAYF_002817"
     CDS             complement(1031..1381)
                     /locus_tag="ACHAYF_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580166.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2678)
                     /locus_tag="ACHAYF_002818"
     CDS             complement(1347..2678)
                     /locus_tag="ACHAYF_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8580167.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2934..3500
                     /locus_tag="ACHAYF_002819"
     CDS             2934..3500
                     /locus_tag="ACHAYF_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8580168.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3645..3732
                     /locus_tag="ACHAYF_002820"
     tRNA            3645..3732
                     /locus_tag="ACHAYF_002820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3677..3679,aa:Ser,seq:cga)
     gene            4066..>4635
                     /locus_tag="ACHAYF_002821"
     CDS             4066..>4635
                     /locus_tag="ACHAYF_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM8580169.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaaacac ctgtaaaacc aagataaaaa tactttcttt tctgtgaaac gattcgtttt
     2641 tgaggatgaa tataaataac gtcagtttcg ttttccatta cacaccttct ttttcatttt
     2701 ctttaactat aacttgaaat gaaagatttg gacaactatc atttaggtca aaactaaaaa
     2761 tgtaagggaa cccattgaat ttaaccaaat ttttttaatt ttgcaaaaaa aagttaaaga
     2821 agactgaaaa aaaatttaaa atataacgtt ttgagtaaaa tgtttgcaaa aaatgctaaa
     2881 aatagggtac aataaaacaa catatactgc agtaaagaag tgggggagtt ttaatgagaa
     2941 gaaaagtcta taccaaggat caaattctaa aagctgctta tgatgttgtt gcaaaggaag
     3001 ggtttaaagg atttactgcg agaaatattg ctaaaaagat ggggatttcg acacagccga
     3061 tttatctgga attcaaaaac atggaagatt taaaaaatac gttgctggat tctatctttg
     3121 aacaactaaa agaagatgtt ttccctattg aacgtactgg tgataaaatc attgatctcg
     3181 gtttgaacta tatctacttt gcaaaaaagc atcatagtct ttacatggca ttgtacattg
     3241 atgaacatgg ggacggaaag aaaatgcatg atttttcata caattatttc cgtgaaatag
     3301 ttaaagaaga tgaaagatac aatcaattat cggatgaata tgtcgatgct ctgcatactg
     3361 gtacatgggt agcagtgact ggcttagcgt cattgatgtc atcaaacatc atgcatccaa
     3421 cagaagaaca aatcattgca ctgatcaaac ggacaattga ctcgatttta gcattagata
     3481 atcctgaaat aatcagatag tgttctatca gtatgaattt gatcggtaag tccgttggct
     3541 gcaaactgtc aatggacttt tttgctaaaa aaagcgattt ctgttattta tctattagaa
     3601 ataggcttga catttcgtga aaaaagttta aaaatagtac ttacggaagg ttggcagagt
     3661 ggtaatgcac cggactcgaa atccggcgaa ccggctaata ccggcgcgca ggttcaaatc
     3721 ctgtaccttc cttatatcaa cgattagagg ttgtgaagaa gctgtcctgc atcaagtaat
     3781 aagaacggcc aaacaaaaat agcttttcct attttttcgt ttgattgggc ttattaccgc
     3841 agatgcaagc ttttgaacac cgtttgttcg gaataagggg ctgtgacaag acttttgtca
     3901 cagcccctta ttgttgtact tttaacctac aatatttttt gattccccct gagtactatt
     3961 ttttataaat gctagtaaat caatcttctt tggccttttt ttgataaaaa aaatactaga
     4021 tgtaagataa aagtgtaaaa acgatttgtt ttaggagggg aaacgatgtg taccgcaatc
     4081 acttatgcaa cgaaagacca ttattttgga aggaattttg attatgagat gtcttataat
     4141 gaagtggtca ctattactcc aagaaattat cgtttcgatt ttagaaaggt aaaaaattta
     4201 gataaacatt atgcaatgat aggaatagca gctggggtat ccaactatcc actctattat
     4261 gaggctacaa atgaaaaagg gttgagtatg gcaggactga attttcctgg caatgctgat
     4321 tataaagaat tacaagaagg aaaagataac gttgcaccat ttgagtttat tccatggata
     4381 ctagggcaat gctcaaccat agacgaagct aaagaattat tggctactat caatctagtc
     4441 aatatcgatt ttagtgaaaa actacccttg tcacctttac actggttatt agctgataaa
     4501 gaaaaatcga tagtaattga aagtatgaaa gatggacttc atttatatga taatcctgtc
     4561 ggtgtgttga caaataatcc gccatttgat tatcaattat ttaatctgaa caattatcgc
     4621 tcgctatcaa atgga
//
LOCUS       DAYYCQ010000140         4628 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000140 DAYYCQ010000000
VERSION     DAYYCQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.145"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACHAYF_002822"
     CDS             complement(52..522)
                     /locus_tag="ACHAYF_002822"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8580170.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACHAYF_002823"
     CDS             complement(681..1139)
                     /locus_tag="ACHAYF_002823"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8580171.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACHAYF_002824"
     CDS             complement(1310..2455)
                     /locus_tag="ACHAYF_002824"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8580172.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACHAYF_002825"
     CDS             complement(2468..3385)
                     /locus_tag="ACHAYF_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8580173.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACHAYF_002826"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACHAYF_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8580174.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1450 a          845 c          876 g         1457 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaa
//
LOCUS       DAYYCQ010000141         4587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000141 DAYYCQ010000000
VERSION     DAYYCQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.146"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHAYF_002827"
     CDS             186..2855
                     /locus_tag="ACHAYF_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8580175.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHAYF_002828"
     CDS             complement(2946..3560)
                     /locus_tag="ACHAYF_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8580176.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHAYF_002829"
     CDS             3680..4219
                     /locus_tag="ACHAYF_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580177.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHAYF_002830"
     CDS             4216..4554
                     /locus_tag="ACHAYF_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8580178.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggaggaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DAYYCQ010000142         4552 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000142 DAYYCQ010000000
VERSION     DAYYCQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.147"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..2034
                     /locus_tag="ACHAYF_002831"
     CDS             94..2034
                     /locus_tag="ACHAYF_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8580179.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2121..4043
                     /locus_tag="ACHAYF_002832"
     CDS             2121..4043
                     /locus_tag="ACHAYF_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8580180.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4323..>4552)
                     /locus_tag="ACHAYF_002833"
     CDS             complement(4323..>4552)
                     /locus_tag="ACHAYF_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFM8580181.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1508 a          774 c          937 g         1333 t
ORIGIN      
        1 tattccaaga atttcagtta ttacggaatc attttgtgct taataaaaag aaaccgtttt
       61 tatatactat aaatgtagaa aggcggtagg aaaatgaaaa aaattattgc aatcacatcc
      121 tgtccagttg gtattgccca tacatacatg gcagcagaaa acttagagaa agcggggaaa
      181 gcagttggcg ccgaggtaaa agtcgaaaca catggttcca tcggtattga aaatgaattg
      241 actgcaagag atatcgaaga agcagctggt gtaatcatcg cagcagatac taaaatagat
      301 aaatcacgat tcggcggtaa acctttgata accgtaggtg tgcaagaagg aatccaccat
      361 gcagatgagt tggtgagaga tattttagca ggaaaagcgc cagtctataa aagtacggag
      421 gcaattattt cttccgaaaa taaaagtgaa tcagaaaatt taggtaaaaa aatctataag
      481 agtttgatga atggcgtttc gtacatggta cctttcgtag ttacaggagg gttattgatc
      541 gcgatttctt taacattagg aggaacagct acacctgaag ggatcaagat accagaagga
      601 acgatttggg caacgatgaa tagtatcgga agtatcgcta tggggttgat ggttccaatt
      661 ctatctgcat ttattgcgca aagtattgca gatcgtccag gacttgttcc tggatttgtt
      721 ggtgggatgt tagcggcaaa cggagcatta tatggtagtg atgcgaatgc gggattctta
      781 ggcgggatca tcactggatt tttagcagga ttcattgctt taggaatcaa gaaagtgcga
      841 gtaccgaaag ctttacaatc gatcatgcca attattgtta tcccaatttt aggatcattg
      901 gcagttggtt ttgtgtttat ctatgttatt ggagaaccag tagctcttct atttagttca
      961 ttgactcact ttttagcagg gatgcaagga ggaagtgaaa ttgttctagc tatgatctta
     1021 ggagcgatga ttgcttttga tatggggggg ccgttcaata aagtagcctt tttgttcgga
     1081 tctggcttga ttgcagaagg caattattcc attatggggc caattgctgt agctatctgt
     1141 atccctccat tagcattggg gctggcatca ttgattttaa aaaccaaatt tacgaaaaca
     1201 gaacgagaag ctggtaaggc gtccttagca atgggactgt ttgggattac agaaggcgct
     1261 attccgtttg cttcaacaga tccactacgg gtgattccaa gcattgtaat cggggcaatg
     1321 acaggaagtg tgatcgcgat gctttcagga gtgacagatc atgtagctca tggcggtccg
     1381 atcgtagcgg ttttaggagc agtggatcac gtagccatgt tttttgtagc agtgattgct
     1441 ggtattgctg taaccgtgct aatgttaaga atccttaaac cgacattaca acaaactgcg
     1501 gttgctgcag caaatggtgt gacagtagca gatctatcag agactgaaac aagtgatgtt
     1561 tatacgaaaa ctgaaacttt tgattctatc acggatatta tgtcagatga agcaatcgtt
     1621 ttaggtgaaa cagcaacaac aaaagacgga gtaatcgaag atttagtcca acgtttagaa
     1681 aaaacagcgg cgatcacaga taaaaccaga tttaaagaag tcatctatga tagagaacat
     1741 gaaagtacaa cgggaatcgg tatggggatt gctattccac atggaaaatc agcttatgtt
     1801 cttaaaccaa cagtggcatt tgccagaagt caatctgggg tagattggca ttctttagat
     1861 ggaaaacctg cacatatgat ctttatgatt gctgtacctg aaaacagcca aggtgatatg
     1921 cacttgaaaa tactgcaacg tctatcacgt aaattgatgg atgatgactt ccgtcaagca
     1981 ctgatggaag cgccagataa aacaacagtt caccaattat taagcgaaat gtaacataag
     2041 atgaaaaatc gaccgtgatg agacataata gatgtgttat catgatcgat tttttattta
     2101 gaagggagtt tctgcacatt atgaatcagc gtcagcaatc tttgatctat caattaagtg
     2161 agaacaataa ttatataaca acaaaaaagc tagcagagca tttccatgtg tcagaaagaa
     2221 cgattcatag cgatttgaat accattgaaa cttggttgag agacaaacat ctgcctttaa
     2281 tgtttgaaag aaaaaaaggt acaggtatct tgttgaatgg tctgcctcac gaaagggaac
     2341 aactgaaacg agcgcttaat gaagagactc aaacagaaat ggataaaaaa cagcttcgtc
     2401 agttattgat tttccatctg ctagtagcaa aagacggatt tagtcttgaa gaactttcag
     2461 aaaaactata tgttggaaaa cgtacgattc gtaaagaatt gacagagttg caaagctttt
     2521 ttgagtatca ccatttacag ctagtatcca aaacaaaact aggaactttt cttaaaggcg
     2581 atgaacaaga aaaaagacag ttgcttgtta agacattacg aaatatgcag aaagaagatc
     2641 cacaaagtcc gtcgttgaaa gaattcttcg ctaaagatac attaaaagtt atccaacata
     2701 cattacaaga tgttttttat gaaaaccatt tagaacagcc gaatggactg gctagtgtgg
     2761 agattcacat ctactttatg ctcgagcgaa tgaaacagta ccagaaagtg aaattaagta
     2821 aagacgaaaa cgaagtcgtg gaacacaccc aagcacaaaa gttaagtagt caaatattag
     2881 caaaactagc caccatctat ccaattgaat tttctcctga tgaaatcaat tatctggctt
     2941 tgcgtatcgc caatcttttt acaaattcac aagcagccac acgttttcaa aaggaatcta
     3001 gcgcacttgc tgatcatttg atcgaacaag tggaacagtt tctaggctat tcattaaaag
     3061 aagatcagct actaaaacaa aatttaaggt ctcatttatc ttcaacttat tttcgactcc
     3121 attatggttt gcagatttct aatccattga cgaaaaatgt gtttagtacg tacacacaac
     3181 ttttcttagt tttacagtta attttagaag attattttga aaaggaaaat ttttatgttc
     3241 cacaagagga gacagcttat ttaacgatcc atttccaagc agcgatcgag cgacacaaac
     3301 atcagaaaag tagaagatat caaacggttt tagttagtga atattcaaaa gctatggcta
     3361 cttttctcga agcacggctg aacagagaac ttccagaatt aacgattatg gatctagttg
     3421 aatataagcc agaattaaag agacaagatt ttccctctgt tgattttatc ttggctacgt
     3481 taccatttaa acatataagc attccagtaa tcgagatctc accaatgatc acagaaactg
     3541 atctagccta tttgacaaaa tatatgctgg agcatgtgcc tatcaagaag aaaaaaacat
     3601 ttgatctagc aagctttact catccatttc tgatctttcc tcaacttgaa cggacagacc
     3661 cagccgatat tttaaacttt atgggaaacg tcttagtgga acatcattat gtggagccgg
     3721 aattcatcga ttctgtcttg gaaagagaca gtcactccag tactagagta gcaccatttg
     3781 taacaatccc tcacggtaat ccgctgtatg tgaagcattc gatgatttct attgctacaa
     3841 tgaaagaacc agtcctatgg cacggggaac agatacgtat cgtactgcta ttagcaataa
     3901 agaaaaatga tttgaaacat gcagagttca aaaaaatatt ttctgttatt cactatctag
     3961 aaaagcagcc agaacaaatg gctcagttga tgcaatccaa tcatccgcta gaaatcttaa
     4021 cgttgttatc tagttatgaa taagtagcga ttaaaaaact gcctttatat ttataataat
     4081 tctatctatt tatttggaag tagaaaacta aatacaaaaa aagaggatgt ggccaaagtt
     4141 gtggcacatc cttttatttt gagtttctta taatttttta tagttgctta cgatgtcttg
     4201 actactggta aacggtgttc aagagacaat ttgtcggcaa taaggcacaa ttttgtaaaa
     4261 tatggaaagc tattttttta acattgtttc ttagtgcttg aaaaatcatt ttgaatactt
     4321 ctttatacga tcgatgtacg ttgatttaag tctaaaactt ttcttttgat tccttctctg
     4381 acttttctga attccgttaa tatttcttct tcgctcccga cagcttgtgc aggatctttt
     4441 aggtcccagt gaaggtgttc gtgattgttt ggaacggcag ggcacttgtc tttggcatca
     4501 ccacaaagtg taatgatcaa gtctgcctga gtaaagtaat ctctatcaat ca
//
LOCUS       DAYYCQ010000143         4433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000143 DAYYCQ010000000
VERSION     DAYYCQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.148"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHAYF_002834"
     CDS             complement(543..2048)
                     /locus_tag="ACHAYF_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8580182.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHAYF_002835"
     CDS             complement(2045..2731)
                     /locus_tag="ACHAYF_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8580183.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHAYF_002836"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHAYF_002836"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8580184.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          730 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAYYCQ010000144         4418 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000144 DAYYCQ010000000
VERSION     DAYYCQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.149"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACHAYF_002837"
     CDS             59..913
                     /locus_tag="ACHAYF_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8580185.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACHAYF_002838"
     CDS             1010..3259
                     /locus_tag="ACHAYF_002838"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8580186.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4418
                     /locus_tag="ACHAYF_002839"
     CDS             3262..>4418
                     /locus_tag="ACHAYF_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8580187.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1556 a          625 c          807 g         1430 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagt
//
LOCUS       DAYYCQ010000145         4414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000145 DAYYCQ010000000
VERSION     DAYYCQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.150"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..105
                     /locus_tag="ACHAYF_002840"
     CDS             <1..105
                     /locus_tag="ACHAYF_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8580188.1"
                     /translation="MEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            1492..2415
                     /locus_tag="ACHAYF_002841"
     CDS             1492..2415
                     /locus_tag="ACHAYF_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8580189.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            2431..2970
                     /locus_tag="ACHAYF_002842"
     CDS             2431..2970
                     /locus_tag="ACHAYF_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8580190.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3655..3828
                     /locus_tag="ACHAYF_002843"
     CDS             3655..3828
                     /locus_tag="ACHAYF_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580191.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYN
                     SPNEELFVKETDK"
BASE COUNT         1638 a          679 c          742 g         1355 t
ORIGIN      
        1 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
       61 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag cttaaatcat agataccgta
      121 agggatttta ttctttattt aaaactttgc aacagaacca tcatatcaat acaatgatta
      181 cgctcgttat gtaataaaat aatcagaaag tgaaactatc tcatgaataa aaagatttta
      241 gctgtaatta tttcaataat agttttaatc actatgttct tcatatttag gctaattttc
      301 aatataagct tacaaaaaag tattctttat ctaattccta tagcatttat cttagcaatt
      361 tttagaagta tttacggaaa taaaaactct aaatagctaa aaataaatca tactaaattt
      421 tacaaaaagt ggctagcatt ttctagccac tttttgtatt gtggagcgtg ggttttgagg
      481 gggattcaag ggggagcttg cgcccctttg acaagcggtt atttgcacca aatacggagg
      541 tatttggcta gcaaatatag agcttgccaa acccttttag tgagttttgt agaaagtacc
      601 tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa agggtgggac
      661 ttatgagcaa attaatgcaa aatttggcta gcaaaaaagc taaaaaatat atgctaataa
      721 tcgagcgaaa taaacacttt ttcaagtgag cgaatccgcc atgacaaagc tgaagcagtt
      781 tatttcagct ttgtcatggc gtgtgccgac ttttttaaaa gtctaggcac acgagcaaat
      841 atctttatca agctctaatt tgcgttttaa gacgtttgaa acgcctaagt atataaatat
      901 ataagaatag gtttaagagt gcttaaatcg aattttagag ggcatttaga caattttagg
      961 gttgttaggg cttgccctga ccgcctgtaa ggcgttttaa gaacaagata agggatttat
     1021 actaggactg tcttaaaatg tcttaaacga acgctcagag cttctcaaag ggttccttat
     1081 gaacggcttt tagcaaagga gccggaggcg actgcgcagt ataatgtgag agcagagcga
     1141 tcacaccttt ttagcgaacc gtagggagca aggtttggaa ttggcagttt tgataaattc
     1201 gcgaacctgt ttagagaaaa aaggagtgat tttttaggcg ggaaaattag cctcccctct
     1261 ttttatatct ctttttatat ctctttttat atctctttta tgtcggctgt gtccttactc
     1321 tcccaagcaa ttcagagttt tttcaagtag gttttctacg cttttcaagt aggttttcta
     1381 cgcttttcaa gtaggttttc tacgcttttc aagtaggttt tccgacatta ttgagtattt
     1441 ttataaagta tgttactttt atttcatact ttataaaagt aggagaagct tatggcaaat
     1501 gaaattgtaa aatatgaaaa tagattaaat catattcctc tgagaaaatt taatagtcgt
     1561 gaaatgaact tatttttttc aattgcttca cgtgtacgtg aaaaaggtgt agatgagatt
     1621 attttttcat ttcacgattt gaaaaaacta agcggttata cagaacgtgg ggaacaattt
     1681 ataaaagatc ttagtagcac gtatgacaaa ttattaagta ttaatgcttg gaatgatgat
     1741 ggaaatactt taactaaatt tgttgctttc acaaaatata gcattatacg agataaacaa
     1801 cttgttataa tttcagttaa tccagaattt aaaggacttt ttaatcaatt aaagaactgg
     1861 acaagattta atctagagca atttactaat ttggatagca catattcaaa aactatgttt
     1921 cgattattaa aacaatatcg aactcaaggc tggactgaat tttccaaaga aaaatttttt
     1981 gatttactag atattcctaa aagttattgg aatagtccgt caaatattga caaatttatc
     2041 ttaaggccaa ttaaagaaga actcactccc ttatttaaag gattaactat tcgtaaaaaa
     2101 tatggtaaag gtcgaggtaa acctgttatc ggttatcgtt ttacttggaa agctgaaatt
     2161 aaccatgctg acgatttttc caaagggaaa caagaagatt tacgtataaa attatttaat
     2221 attgatcaca atggagaatt aacacgagaa gaaaaatggc gcgctaaaga tcgaatacta
     2281 aatcttccct taggcactca tgaagctgat tttaaacaac aacaacaaac tgaagaaaaa
     2341 caggcagaaa aacgagaact ttcaaacaaa ctcaaacaag atttactagg aaatttacaa
     2401 aacttattcg attaagctta aggagtattt atggcagata aaacaatcaa agaacttgcg
     2461 gaagaattag gagttagtaa aacagcaatt aacaaaaaag tttctgacaa aaatcgaaaa
     2521 ctttggtttg caaaaaacgg aaacaagttt gtgattaacg aaaccggtca aaaagctata
     2581 aaatcaatgt ttttagtcga taacgcaaac ttaaatcgaa aactagttgg cgaaaaatca
     2641 aaaaccaaaa accataaaaa caccaaccaa gtttccgaat taaaactcct ttctttccta
     2701 gaaaaacagt taaatcgaaa agatatacag ctacaagaaa aagataatca aataaaacaa
     2761 atgcaaaaat tactcgacca gcaacaacaa ctcactctac aagcgaacca acaaattgag
     2821 aacctacaag aacagctcca tttaacttat acagaagaaa ctccaacaac cgaatcaact
     2881 gccttttctg aaaaaaaagc agacaataca caaccactta ttgaaaaaaa gtggtggcaa
     2941 atttggaaat ctaatagaac caatctctaa aattttctag aagttagttc ggatagtaga
     3001 ggaaaaagtt gtactacctg atggggagat cactcaaatt tttatgagaa agaaaaaata
     3061 gtttacaact gtaaacaatc aatgtttata gttgtaaact atcaaactat atggtgactt
     3121 attgcttaaa ataggttgaa atctcactca atttttggtg gaaaaacaaa ataaattcta
     3181 atcgattttg ttgaaaaagt cataaaaaaa caaatgtgat tttaacaaaa tttcaaaaaa
     3241 atcgtttgaa atttcgttta ttttttttga gaattttgat catcgtaggg tcattgtaga
     3301 atcattatga gatcatcgtg ggatcatcga ataaagtatg ggaaaggctt gcggtgcttg
     3361 gtattaaggg cattctccgc ctcccaggtt cttgaccatt ttctctcaac gtggtaaaaa
     3421 tcccacttat aagagaaaat ggtcaagaac ctaaacggtc ggcgaagaaa acataccaag
     3481 caccacaagc aaaaaaagtt agagtaactt ccaaaaaaga aaaatttttt agaacaactt
     3541 ttgaaaattt aattttgatt tatatccgtt caatttaaac atttgataaa ctgaaggcta
     3601 gtatttaaaa tgtgtataca agtggtagcg ttaaaaaaag tggaggtata acctatgtta
     3661 aaaaaaactg tgttggtact gggttctatt tctctaggaa tattaggttt tagccccatg
     3721 ctattatccg taattggaaa aaacaattcc aacggagaac ttaaattaaa ttcaaattat
     3781 tacaacagtc ctaacgaaga attgtttgta aaagagacgg ataaataata aaaaagactg
     3841 ctctttcgag cagccagtta aaaaataatg agtgtttatt cttgaaaaag caacgcgctt
     3901 atgaagcgct accaaaaatt tatcatattt tatgaaaaat aacaccccag taaaaaatga
     3961 ctggtaaaaa ttcacaaaga aacggaaaga agcaactgca gaaaatcaag ccttaaaaag
     4021 agcttttgac ttagaccaat cgaaaatcac aaagtgagcc cctgtatccc ttggtagaga
     4081 ggctacttgg catagtacac attatgtcaa caagaattta atataacata tttttctagt
     4141 ttgttataac aatgccactc taacaaaaat gtaagcatta tccttattaa aagctccttg
     4201 acaagactat accttttatt ttatgatttt tacatagaaa acgttttctc tattttttgt
     4261 ttagaggtga tttttatgat ttgcaagaac aagaaaaaaa ttattgctat tattgctagt
     4321 ttctctttga tttttagtac tgcatgtact gtcttattaa caaataatgc taatgttgaa
     4381 gctgcaagct cttcttacaa acataatcat gctg
//
LOCUS       DAYYCQ010000146         4285 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000146 DAYYCQ010000000
VERSION     DAYYCQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.151"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..1887)
                     /locus_tag="ACHAYF_002844"
     CDS             complement(121..1887)
                     /locus_tag="ACHAYF_002844"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8580192.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1847..3601)
                     /locus_tag="ACHAYF_002845"
     CDS             complement(1847..3601)
                     /locus_tag="ACHAYF_002845"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8580193.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3711..4280)
                     /locus_tag="ACHAYF_002846"
     CDS             complement(3711..4280)
                     /locus_tag="ACHAYF_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8580194.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1450 a          809 c          599 g         1427 t
ORIGIN      
        1 ttcaatcata ctgattcttt cgtgtattca aaattagatg ctgtttaata aaattattct
       61 ttttgaaaaa gaattataac tatatcccaa aaattgtagg ttaatttaaa gcctatttca
      121 tcatcgtgat tgagcttctt tttcatagac gaatttctca gatagtttag ttttctttat
      181 gagatttttg tatatttctg accttcgata caactcatta tgagtacctt gatcaactag
      241 ttttccttga tccatcacta caattttatt taccttttca atagacttca agcgatgcga
      301 tataattagt actgtcttat cttgtattaa acgatttaaa ctttcctgaa tacgtttttc
      361 attttccgca tctaaactgg cagtaatttc atctaaaatt aaaattggcg catttttcaa
      421 aaaagcccgt gctatggata ggcgttgtcg ctctcctcca gaaagtgaac ttccgttttc
      481 tccgaccaat gtatgataac cgtcaggtag tttttcaata aagtcttcgc agttagctaa
      541 acgagctgct tttttcactt cctcatctga cgcttgacta tttccaatac gtatattttc
      601 caaaatactt gtattaaaca aagttacctc ttgaaaaaca atagcgattt tgctaaacaa
      661 agaagctgga gatacacgtt taatatcata cccatcaatt aaaatacagc cttcatcata
      721 atcatacagt ctagacacta atttcaagat actagttttc ccggaaccac tagcacctac
      781 taacgcagtc acttctcctt gtttagccgt aaaagagata tgatctaaaa ttggggtatt
      841 gttatcatat gagaaagaga catctcgcaa ctcaatgtca aaagatttca gaggactatc
      901 tgatccctct tggatactgg tttccttcat agctctaatt cgctgtattt taggagctaa
      961 ataaaatatt tcaagcactg cttctttcat agaatcgaag gaatccttta tttttattgc
     1021 ggctagtaag taaccaacga cgtagagtat agtcacttca cccgttaaca gtaagtgaac
     1081 acctactaga atgactactg ctaaactaac atatgagaaa atagaggata aggccatgac
     1141 tgaaaaagta cttaattcta cctttaaatg aatcctttca ctctcttcca tttttttgaa
     1201 aagtctgtct tgaacttttt tagatagatt aaagctatta atctcttgct gcaattctat
     1261 agtttcttga aattcttccg agttctctct caaagtatcg taatatttag tattggcttt
     1321 cgtttgggat ttctttgata acaagattaa gacaaaacta aataacgttg gcaaaataac
     1381 agctaatccc attttgacat taccaaccaa aagcatcact gaaataaaag ggaaaaacag
     1441 agccataccg ccggctttag gtattgcatg actcatcgca tgctcaatac cttcaacgtc
     1501 agacatgata gtttgagata aatctgataa attatgtttt gaaaaatagg ataatggtaa
     1561 atttgacaaa tcgtctgcaa tttgcactct taaatgcgca ctttctttat aagtactatt
     1621 atataagttt tcatactccc tatctaacaa ccaatacatg aaaagcaaag taacaaacga
     1681 tacggcaaaa taaagccaac ggggctttgt attttctaaa actaattcgt ctatcagcat
     1741 cattataatg aatgctggta ccatattaat gcagtataca aaaaaactag aaatagaagc
     1801 tttagttaaa gcttctgcgc cctgatcact taaagcaaat ttgtttttat aaaaactttt
     1861 cattgtcaag cctccaatca tttgcattct cataattagt gaccaattgt ttatacactt
     1921 gatgtttagc cattaattca ttatgatttc cacgttctac tattttccct tctgacaaga
     1981 caagaatttc atctacagat ttaatgctgg ataatcgatg agctatcata ataactgttt
     2041 tttctttcat cagatgttga aaagcctttt gtagttcata ctcattatct ggatcaatag
     2101 atgcactggc ttcatccata ataacaatct ttgcatcttt taaaattgct ctggcaatag
     2161 ctatacgttg tttttctcct cctgacagat aaactccttt actaccaatt aaggtatgct
     2221 ctctttcggg aaatttttct aaaatcgatt cgcaaccggc taatcttagc gcgtcaaata
     2281 ctttacttgc tggcgcttga ggatttgcta tagaaacgtt atcgtaaata ctctgattga
     2341 atagtttcgt atcttgaaag acaaaagcaa ttgtgttaat aattgtgtct tttgaatagt
     2401 gatttaaagg cttaccacca attttaatct gtcccttatc cacattgtag aaacctgaaa
     2461 ttagtttagc tatcgtactc tttccgcttc ctgattttcc tactaatgca taaattcttt
     2521 tttgttctaa aactaagttg aaattttcta aaacacactc ttctttgtat gaaaagctga
     2581 cattttcaaa tgcaatgtca caatttttaa tctgtgtctc gttcccatat tttagttggt
     2641 cttgcttcat ttctaaataa agggagtcta gtgtttcgac tgcatatctt gcttcaaaca
     2701 agtgcatact tacatacatt attctcatga aagaaacaaa aataattcca cttaaaaaaa
     2761 acaacatcaa caagtcaaca gctaatatct tgggagtggt aagagaagtt ataaaaaagg
     2821 ttataggaac tatcaaaatg caaactatgc caaaaaatag ccattgaaaa agaacaaaag
     2881 gtgttttccc actcaaagaa tacttatatg caaacttagc ataatcgtta attgctttat
     2941 tcaaggcttt aaatgatgtt actttagcac caaatatctt tataacttga attccgcgga
     3001 tatattctac cgtttcagag cttaacacat ctaacgattc ttgatatttt tgaataaaat
     3061 ttgtattctc tgtcattttc tttaacaaga atacaccaat tactaacatg gcgattagaa
     3121 ccaatccaat tttaaagtta atgaaaaagc ctaaggcaac tgataaaaca ggtgttacta
     3181 tggcttgagc actatctgga atcaagtgag caattgccat atgtgtttta gccgcgttgt
     3241 catcaattgt ctttcgaata tatcccgaag aatttaaatc aaaaaaacga aaacttgctt
     3301 ctgttaaacc gctaattccc ttttttctca aattagtctc taagcgaaac ccaaggatat
     3361 gagaaaataa gccagaaaaa atataaaata aagctcctaa agttaaccaa gcaacagttt
     3421 gaaccgcata actcttagct aaatattcat ctcctacaat aattagagac tgaagaaatt
     3481 tgtaaatact gtaatatcca tagactgtta atacgacaga aaaatcagaa aataaagtgg
     3541 ctaaccatcc gtagtatttc attttaggga cataactaaa taattttttg tatactttca
     3601 ttttttcctc ccccgcattc aatttcttct cttattcgtt tacaactgtt aatgctttcc
     3661 gtaatagcgt aaattagaga tgtacaaaca gataaaaggt aaactacgtt ctattttttg
     3721 tattttttta agacttttct tccaatcaat acaccaatca tagataaact tgctacaact
     3781 aacactgaaa tgattcccca tatacctacc gcatagtgga tcattgcatc cattgttgca
     3841 gcagattgtc ctttatctat ccaatcttga cgaaaacgat ctgtaaagaa ccatatagga
     3901 atccactgtc cggcagcact gcctaattgc attagagccc aagacgcaac ttgaggaatt
     3961 gctcgatcat atccaaatac agatagaata atatcagcta agatcgccat actaatcgaa
     4021 ctgataaacc aaggtaagta cgccccgccc attaacgtaa aaaataacaa taaaacaatt
     4081 gtgtaaatag taaaaattcc ttttctaggg tagttatagc taatataaac aaatatgatt
     4141 ccagataata atccaaaaat cccgggggat atgctgtgac caaacgcccc accaatacta
     4201 ctaaccatta ttgaaaaaaa gtaaattact agggaaataa ctgtagctaa accaataaaa
     4261 atataatctt ttgttttcat atata
//
LOCUS       DAYYCQ010000147         4062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000147 DAYYCQ010000000
VERSION     DAYYCQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.153"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..1430
                     /locus_tag="ACHAYF_002847"
     CDS             81..1430
                     /locus_tag="ACHAYF_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8580195.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1558..2148)
                     /locus_tag="ACHAYF_002848"
     CDS             complement(1558..2148)
                     /locus_tag="ACHAYF_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8580196.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2295..3647
                     /gene="brnQ"
                     /locus_tag="ACHAYF_002849"
     CDS             2295..3647
                     /gene="brnQ"
                     /locus_tag="ACHAYF_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8580197.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1169 a          744 c          789 g         1360 t
ORIGIN      
        1 tttggaatct tagttgtgtt tttcagaaaa ctctcttaat atggaagtaa ttaaactttt
       61 ttagttagga tgatttaaaa atgaatgaag agcaagaact gcagcgtgga ctgaaaaata
      121 gacatgtcca actgatttct attggcggag cgattggaac ggggttgttt ttaggttccg
      181 gaaagtctat ccagctagct ggcccttcga tattgctcgc ctatcttatt actggatgca
      241 tctgtttttt cattatgcgt gcgttaggcg aacttctgtt atcaaacacc aataaccatt
      301 cttttctgga ttttgttgca gaatatcttg gcaaaaaagc ggctttcatc actggctgga
      361 cgtattggtt ctgctgggta tcgattgcaa tggccgattt aacagccatc ggaatgtatg
      421 tacgctactg ggcgcctggt gttcctcagt ggcttcctga actgattgca ttaggtcttc
      481 ttctttgctt gaatatggtg gctgtctcat tgtttggtga gttagagttt tggtttgcat
      541 tgatcaaagt tgtagcgatc attgccttta tcctcgtagg agcttatatg atcctcaccc
      601 actatacgac agatattggc acagccagta tcacgaatct atggtctcat ggcggattct
      661 ttccaactgg tgcaaaagga tttatcttag ctttccaaat ggtaaccttt gcttttgctg
      721 ggatagaatt agttggatta gtcgctggag aaacagaaaa tcctgaaaaa gtgttgccgg
      781 aagctatcaa taatattcct atcaggatca tcttatttta cttaggatct ctttttgtca
      841 ttatggcaat ctatccatgg aacagcttga atccagataa cagtccattt gtagaagtat
      901 tttcggaaat tgggatcacg attgctgctt ccttgatcaa tttagtcgta ttaagtgcag
      961 cagcttctgc ctgcaacagt gcaatatata gtacaggacg tatgcttcgt tctttagcgc
     1021 aagaaggcag tgcccctaaa aaattcaaaa agttaacaac acatcatgtg ccagggaatg
     1081 ctttgacatt ttctactatc gtcattttca tttctgttat cttgaattac gtgatgccta
     1141 gtgaagtatt cacactagta agcagtattg ccactacttg tttcttgttt atctggagta
     1201 tgcttgtcta cacgcacatg aaatatcgta aaagtatcct cggcaaaaaa gctcattcat
     1261 ttaaaatgcc gttgtaccca tttagtaatt atctggtatt tctttacatg gcctttgtgt
     1321 gtgtcgtctt gttcctagga aaagatacga gaatcgcttt attactaaca cctgtgtggt
     1381 tcttgcttct attactaatc tatcatatga aatatgaacg taagaagtaa gaaagtagtt
     1441 gtgacagaag tggtcagctt cgagaaataa gaaggaattt acgaaaattg cttcttaaat
     1501 ttttgtgaaa tttcggctta tttcttggag ataaaagctt ctgtcccagc ctcttttcta
     1561 tttccccgga aacaaccgct gataataggc atctgctttt ttataggaaa aaatccaaat
     1621 accagataca aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga caccggcaat
     1681 caagcataag ttaatcatca tggagataag acttcgacgg tcctttgaca aaactctcac
     1741 gacatgggaa tctggtttat ttgctttcat gatttgcctc atcaagatac caaaagaaaa
     1801 actccacatc gtgtatacta agacataagc catctcaggc caaaaagctt cagggttacg
     1861 cccgacccaa ctagtaacag aaggacataa agtcagccaa aagagcaaaa agatattcga
     1921 ccagaccact cggtaattaa ttcgttctgc catctgaaat atacgatgat ggttcaccca
     1981 gatcaccgca acaaatataa agctgatggt ataagctaat aatgtatttc ggatgaccca
     2041 taaagaatga aagctagatg tattagggat cttgatctct aatacaagaa ctctcaaaac
     2101 aatggctacc actccatcag taaatgcttc taatctcgac tttgtcacga tgattcctcc
     2161 tcagatgatt cagcgctttt attttatcac ttacgttatt aaataaccat cgttttatat
     2221 agggaatttt ctgaaaattg ttgcatattt ttcgaaattt tgttactttt aaaaaaaccg
     2281 taaaggagtt atgtatgaca aaaaaattat catttaaaga ctatcttttt ataggctcca
     2341 tgcttttcgg tctatttttt ggtgctggaa atcttatctt tcccgttcat ttgggtcaag
     2401 aagcaggcgc tgctaccttt tgggcgaatc taggtttctt ggttaccggt atcggtcttc
     2461 ccttcttagg ggtgattgcc attggtgtat cgaaaagctc aggtgtatat gaattagcaa
     2521 aacggataaa tcaaacatat gcgatggttt ttaccatctt gctttactta gttatcggac
     2581 cgttttttgc tttacctcgc cttgcaacca catctttcga gattggttta gcaccattta
     2641 tcgatcctag cagacagacg ctatatctag caattttcag tattgtattt tttgtgctgg
     2701 cttggtggtt ttcaagaaaa ccgacaaaga tattagatta tgttggaaag ttcctgaatc
     2761 ctgcattttt ggttttatta ggtgctttgc ttctccttgc ttttttgaat ccactaggtt
     2821 ctgtaaatca agcgcctatt cagccaaatt atcaagagca tgcttttttc acagggttta
     2881 ctcaaggata taacacatta gatgcgcttg ctgctttagc atttgggatc atcattgtca
     2941 caacaatcca aaacatgggt gtaacaaaac ctgctgaaat cgctaaagac acgatcaaat
     3001 ctggtgcgat cagcattata ttgatgggaa tcatttatac tttgctcgct tatttaggtg
     3061 ctatgagctt aggtagcttt gctctaagtg aaaatggcgg gatcacgctg gctcagattg
     3121 ctgatcatta tttaggtact tatggcagta tcttattagc atttatcgtt atattagctt
     3181 gcttgaagac cggtattggt ttgattacag cttttgcaga aacattcaca gatttgtttc
     3241 cgcaaagaaa ttacgcattc tttgttgcac ttgctagtat ccttccttgt ttagcggcaa
     3301 acgtaggctt gacgaatatc attcaatttt cgttgcctgt tttgatgttc atctatccat
     3361 tagcaatgac actgatttta ctagtgttag ttggtccttt gtttaagcag cgtacggctg
     3421 tttatcgaat gactacttat tttacattga ttgcttcgat ttttgatggg ttgaacgctt
     3481 gcccagaaac tatcaaacaa acacctattg ttcaaaatct tttatatgtt gcggaaagct
     3541 acttgccgtt tttcaaattg gggatggggt ggatcgtacc agcagttatc ggttttgtta
     3601 tcggactgat ttggagtttc gctaagaaag aagaagtagc agattgataa aagaggttgt
     3661 gaaagagacg ttcagccttg agcaataaaa agtaattttg aaaaatggct tctcacattt
     3721 ttgcataaat ttgacttaat accgagaaac tgacttttga acaccgttta ttgagaggtc
     3781 gtgaaaaagc cgcttagttt caagtattaa gaaatatttt gtgaaaatgg cttctcacat
     3841 ttttgcataa atttgactta ataccgagaa actggctttt gaacaccgtt tattgagcgt
     3901 ctataaactg acctgagtat tagagaaaca aaacggcaaa gtagctccat atactttgcc
     3961 gttttgtttt gaactaatac cgaagagaag ctgatcaaac aacatttctt ttataatata
     4021 gattttagac atatatatga tataaaaata gttttcgcaa aa
//
LOCUS       DAYYCQ010000148         4035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000148 DAYYCQ010000000
VERSION     DAYYCQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.154"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1039
                     /locus_tag="ACHAYF_002850"
     CDS             107..1039
                     /locus_tag="ACHAYF_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580198.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            1193..2482
                     /gene="tig"
                     /locus_tag="ACHAYF_002851"
     CDS             1193..2482
                     /gene="tig"
                     /locus_tag="ACHAYF_002851"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8580199.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            2729..3979
                     /gene="clpX"
                     /locus_tag="ACHAYF_002852"
     CDS             2729..3979
                     /gene="clpX"
                     /locus_tag="ACHAYF_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8580200.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
BASE COUNT         1442 a          658 c          865 g         1070 t
ORIGIN      
        1 taaaatctag cttttagagg gtgcttcagt tatttttaat tgtcatgctt tttttgtttt
       61 gctactattg aacatgagct gaatatgagc aagaggagga cactgcgtga agccgattga
      121 ttttccagaa aaatatgaga atttaatgcg agtggcacaa caagcactag caaaccagca
      181 atatcagcag gcaaaagaat tatttcaacg agcttacgag ttgaaagaaa gctttgaggc
      241 taatagcctg cttgtttttt gtctttacga gttggacgag aaaaaagaag cattgaagca
      301 agcactcttg catgaaaaac agtatcttca aaatgaggag tttgctgaat tttattttga
      361 cttgttaatc agtacacaag attttttata tgctagaaaa ttgatagcaa gtacagattt
      421 ttatgaagct ttcgagcagc gggtcattga aaaaatccaa tttgcagaag aattatccgg
      481 acaaatggag aggcagaaga tcaaggcact ccacaataaa tcggaggaac ttccttcgtt
      541 ggaacctgca cgtcagcttg cgtctattga tgagatcgaa cagcttcctt atcatgagtt
      601 tatccaaaca gcgagtaaac ttattgtatc gccagaggtc catattttag caagggcaaa
      661 attactggag acacttaggc aattgaacga atgccatcct gttttctatt tgacgattga
      721 agaaaagtta gtcaaagtca taccaaaaga tctgccaaag ccgcagcagc aggcaagtta
      781 ccagcaatta tgtgtatttt cagatcgtta cgggaatgaa gatgcgctgc ttagttccgt
      841 gttaaaagaa gaattcacgt tgcaatctgc aattgtttat cctgtctatg atacatacat
      901 cgaagacccg aagcgctggt ttcaactgac tgtagaggcg tgtacaggaa aaacgatgta
      961 tgaaggtaca gaagatgaaa aacaagattt tttaaaaaaa agagaaaaaa ttttgcagca
     1021 aatgatcttc ttccattaga ttttatcagt gaaaaagata aaaatgttat gaaaaacaag
     1081 gattctatcc attcaatatg tttacaattt gaaagggtta gtgtactatt taaaggtatg
     1141 catgaaacgt ttttgatgta taaaatgaat ttctaaactc ggagggaatt aaatgactgc
     1201 aacatttgaa aaaaaaggca cgaatgatgg tgttttaaaa tttacgattg cgcaagacga
     1261 aatccaaaaa ggattaacaa ctgcgtttaa taaagtgaaa aaaagcttga acgtacctgg
     1321 attccgtaaa gggaaagttt ctcgtcaagt attcaaccgt atgtacggtg aagaagcttt
     1381 atatgaagat gcattgaatg ctgtattacc agaagcttat gaagctgctg tcaaagaagc
     1441 tggcatcgat cctgtttctc aaccaaaaat cgatgttgaa agcatggaaa aaggtcaaga
     1501 ctgggtaatc actgctgaag taacagtgaa accagaagta aaattaggac aatataaaga
     1561 tctagttgtt gaaaaacaag accgtgaagt aacagatgct gacgtggatg cacgtatcaa
     1621 acgtgaacaa gaagcacaag ctgaattagt tatcaaagaa gatgctgcag ctgaaaatgg
     1681 cgatacagta gtaatcgact tcgaaggctt tgtagatggc gaagcgtttg atggcggaaa
     1741 aggcgaaaat tactcacttg aattaggttc aggctcattc attccaggtt ttgaagatca
     1801 attagttggt cataaatcag gtgaaagtct agatgtaaat gtaactttcc cagaagatta
     1861 tcaagcagaa gatcttgctg gaaaagaagc agtcttcaaa gtgacaatcc atgaagtaaa
     1921 agcaaaagaa gtgcctgaat tagatgacga attcgcaaaa gatgttgatg acgaagtatc
     1981 aacattagac gaattgaaag aaaaatatcg caaagaatta gtagaaacaa aagaaaaagc
     2041 agcggacgat gcaaaagatg aagcagcgat ccgtatggct gttgaaaacg cagaaatcgt
     2101 tgaattgcca cacgtgatgg ttcatgatga agtacatcgt tcaatggacg aattcttgaa
     2161 caacatgcaa cgtcaaggta tctctcctga aatgtactat caattaactg gttctacaga
     2221 agaagacttg cataaacaat tcgaaggtga agcagaaatg cgcacaagaa caaatcttgt
     2281 gatcgaagca attgcagctg ctgaaaatct tcaagcaaca gaagaagaaa tcaatgcaga
     2341 aatccaagaa ttatcagaaa catacaacat gccaatcgaa caaatcaaac gcgtgttgac
     2401 agaagatatg ctggaacatg atgtgacaat gaaaaaagca atcgaattga tcacaggtac
     2461 agcgacagaa gcgaaagaat agaaatcaag taataaatca tttctgaaga agctacttct
     2521 agaaaattgt ttattcgaaa taagggactg tgacacgatt ttgtcacagt tccttatttt
     2581 ttgttaagag taaaaaatgc aaaggtatag gtcagttgat cctttgttaa acactttttc
     2641 tttttactaa actatgttat gatagtaaaa gactgtaaag tctttgatag tttcttgcat
     2701 actttttgca agttaagggg tgaagaccat gtatgacaat ccaagtagca atgagaccgt
     2761 acgctgttct ttttgcggga aaacccaaga agaagtgaaa aagatcgtag ctggtccagg
     2821 ggtctacatc tgtaatgaat gtatcgacct atgtaaagaa atcattgatg aagaatttta
     2881 tgaagaagct gtacgcgaat tcactgaagt ccctaaacca ctagaaattc ttgaagtatt
     2941 gaataactac gtgattggac aagatcgcgc aaaacgctcg ttagctgttg ccgtatacaa
     3001 tcactataaa cgtgtcaatc agcaagcaca agaaaacaca gatgatgttg aactgcaaaa
     3061 aagcaatatc tgcttgatcg gacctacagg gtctggtaaa acatttttag cacaaacatt
     3121 agcaaaaaca ttgaatgtac cttttgcaat cgcagatgcg acaagtttga cagaagctgg
     3181 ttatgtgggg gaagatgtag aaaacatttt gcttaaattg cttcaatctg ctgattacaa
     3241 tgtggaactt gcagaaaaag ggatcatcta tattgatgag atcgataaaa ttgcgcgtaa
     3301 aagcgaaaat gtatctatta ctcgtgacgt ttccggcgaa ggtgtccaac aagccttgct
     3361 gaaaattcta gaaggaactg ttgcaagtgt gccacctcag ggcggacgta aacatccaca
     3421 tcaagaattt attcagattg atacgacgaa catattgttc atcgtaggtg gtgcatttga
     3481 tggcatcgaa acgattgtga agaatcgtat gggcgaaaag acgattggat ttggtacaca
     3541 aaaccaaaaa ctttctgaag acgaaagtgt catgcagcat atcattcctg aagatttact
     3601 aaaattcgga ttgatccctg agtttatcgg acgattacct gttatggcag ctttagagaa
     3661 attgacaaca gatgatctgg tacggatctt gactgagccg aaaaatgcgc tagtcaaaca
     3721 atatcaaaaa cttctttctc tagataatac cgaacttgaa ttcgaacctg aagcgttgag
     3781 agccattgcg aaaaaagcaa tcgaacgcaa taccggtgct cgaggacttc gttccatcat
     3841 tgaagaaatc atgatggatg tgatgtttga tatcccttca aatgaaaaca tcgaaaaagt
     3901 gattatcaca aaagaagcag ctgaattgtc tggtgagcca acagtaatct ataaagattc
     3961 agagaaaaaa gcgggttgat gaaaagaggc tgccgtaaaa gtcattttgg cttttgcgac
     4021 agccgcttgc tttat
//
LOCUS       DAYYCQ010000149         3974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000149 DAYYCQ010000000
VERSION     DAYYCQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.155"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..471
                     /locus_tag="ACHAYF_002853"
     CDS             <1..471
                     /locus_tag="ACHAYF_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580201.1"
                     /translation="IIICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFII
                     FSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLL
                     SLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(597..1274)
                     /gene="nth"
                     /locus_tag="ACHAYF_002854"
     CDS             complement(597..1274)
                     /gene="nth"
                     /locus_tag="ACHAYF_002854"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8580202.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1281..1979)
                     /locus_tag="ACHAYF_002855"
     CDS             complement(1281..1979)
                     /locus_tag="ACHAYF_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8580203.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2247..3431
                     /locus_tag="ACHAYF_002856"
     CDS             2247..3431
                     /locus_tag="ACHAYF_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8580204.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3437..3880)
                     /locus_tag="ACHAYF_002857"
     CDS             complement(3437..3880)
                     /locus_tag="ACHAYF_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8580205.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1041 a          781 c          645 g         1507 t
ORIGIN      
        1 atcataattt gtctgcacca tcttttttta tcgaaaacaa tacctcgtta ttccaagaat
       61 aaaaaagctg aaaaattttg tttattatta aataaattta ctttaattgc tcctatcctt
      121 gcatttatta ttttttctgt acttttaagc actactctta aaggaaaatt tatggaaaga
      181 agctctcatg caatgattct cactttttta tggttgttgt tcaccagaat ctatattttt
      241 ctgatgagcc tgaaaccgcc caaatccatt agtttatgtt tagttataaa tggtattttt
      301 ttattatcac ttatcatttt tattacacct ttagatagat acgtcactta tttatataat
      361 cctttggaat actggactta ttttataggt atattggaag gcattatttt ctacatcggg
      421 tattttccca ataaaaataa caacttttat ttttatcgca ataaattgta ataagttacc
      481 atcctagcta ctgagtatgt acatttactt tttcatgcaa aaaagataaa cgaaacagct
      541 tttctccata ataacaagat aattcataca tttatttgca attaaagaaa ccacgtctac
      601 aggcgtggtt tcttttttag cgccttttct tttagacgca tccgattttt cccatcttga
      661 catattgata gcaaaggaca tacctcgcac ttgggatttc ttgccgtgca atgatatcta
      721 ccaaaaaaaa tcagtgtatg atgtgtcttt acccataatt cttgtggtac tttccgcatc
      781 agtgtttctt ctacttccat gaccgtagca tctagtttgc agatcctcag gcgttttgag
      841 acacgttcca cgtgggtatc tactgcgatt gctggtatcc cgaatgcatc ccccaatacg
      901 acatttgctg tctttctgcc tacaccagga agagacatca attcttctct tgacgttggc
      961 acttgcccat cgaaacgttc aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta
     1021 tttcgataca aaccaatcgt ttttatttta agaatgattt catcgattga agcttctgct
     1081 aacgcatcgg gtgtcggaaa agaggcaaac aaatctggcg ttgctttatt cactgataca
     1141 tctgtcgcct gggcacttaa gatcactgca atcaataact caaatggatt attgtgcttg
     1201 agttctccat gtgcttcggg aaacattcca tacatcgttt ctagagcttc cattgttttt
     1261 tgtttgctta acatattccg ctactcctcc aaccaatttt tcagtgagac tttcggcaat
     1321 tcttcttgtt gagctgactg ttctacttct ctttgaagaa gctgctgttt tcttcgtttt
     1381 tggtcttctt ccacttgttg ttttgtacgt agattttttc tttcccacga tagcaaaatg
     1441 cggtcaacgt aattaaaact ataagcctga tttaatacgg cttcccgcag tgctagcttc
     1501 aaaatttccg gctgataatg gtcttcttct agccattgtc cgatccgctg gaactcgatc
     1561 gaagaaagcg ggcgtccaaa ttcttgttca aataattgat acactgactg gatttctttt
     1621 tcttgtgttt tttcttcgta ttttttttct tgtgttttta aatactcacc taatagatca
     1681 tagatggggt aaagattata tcgatctgct tttttaccgt tgacaatcgt cgtttctatc
     1741 ttgataaatc catttgttac gagacgatcc agtatttgaa aaatctgatc ttgtttgatt
     1801 cccatatcca tagcaatcat ctgcaaatca gggaaagtat ctccttcaag ctgtgccata
     1861 tgcaactgta gaacaaataa aaattcttca gattgtagac cgatcctatg ataattttta
     1921 agtaaaagat tagatatgac tgtttgtcca gcgcttaagt aattttctaa attcaacatg
     1981 atttctcctc cgctcaatca cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt
     2041 cttttctatt tacttcactg atcggtcaga tttacaatgt cgtctacttg atttttgttt
     2101 gttcatgttt ttgatgcgta tcttcacttt tcggaataaa taaattaagt agatttcgct
     2161 gttccttaga atgttgacaa ggaaacaaaa ttactataca ctatttttgt ttccaagtta
     2221 acattattgc ataaaggagt ttttacatgt acagtttcac aaataaacat tctaaagttt
     2281 ttggatacgt ctctgtctta ttcactggat taggatttac tatcgtcagt cctgtacttc
     2341 cgtttttaac gctgccttat tctcactcca tacatcagca agctttctat atcactttgc
     2401 tcatgtctgt ttatgctcta gctgccttct tatctgcccc cattctcggt agcttaagtg
     2461 atcattttgg gcgtcatccg atccttatta tcagtttatt aggatcaagt atcggatatt
     2521 tgctttttgg tttaggaaat tcgatatgga tgttgttcct tggtcgtatc attgaaggat
     2581 tgacggcagg agaaatctcc accttatatg cttactttgc cgacatcacg gaacctaatg
     2641 aaagaaccaa agtttttgga tggatgggtg ctttagtcgg tataggaaca acgctgggtc
     2701 ctattatagg gggtctatta gctgagttgg ggaattctgt tcctatattt attggagcac
     2761 tctttacttt cttaaatgcc gtttatggct acacatttat gctagaaagc cttccaataa
     2821 aaaaacgttc agtagattta agtttctccc atgtaagacc ttttcaccaa ttacaacaat
     2881 tattcaagat ttcatccgtt attcctttat tgacagcagg ttttgctgtt tggttggcag
     2941 ctggctctct tcaatctatc ttttctcaat tttcgattga cgcctttcag tggaaggctg
     3001 ggttggttgg tttaagcttc tctttgattg gaatcttgga tactgtctcg caattgttca
     3061 ttatgcctcg attgctcaaa aaattttcag aacagcagat cacaagaata gggatgttca
     3121 gtgaaatcct cgcctatcta ttcatcacat tatctggaat tcttctactg ccgattttgt
     3181 ttttatttgg gattatctgt tatggtttcg gtgattctat ttttactcct gtattcaatg
     3241 gccaactatc caattctgtc tcagataacc agcaaggtct tgtaatgggc ggtacccaaa
     3301 gtatacaatc tctatcgcgc gtcatcggcc cgctaatcgc aggacagctt tatgctgcag
     3361 caccatttct tccgacggta ttcggatttt tgcttatgat ttttgcctat tatcactaca
     3421 ataaaaaata gtagcttcat ttgctacttc gtataaattc tctgtatcga ccgataaaac
     3481 gaaatactgt gtcaaattct tcttttgaca tattttcaaa tacagtagca tctcgctgta
     3541 cccatttttg atggagttgc tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt
     3601 gaaaatagat ttctttttta ttctcttcgc tttgataagt gtttatgatc cctctagcta
     3661 tcagtttttt tgttagctta ctgatggctc ctctagtcat gtaactagca ttcgctaatt
     3721 ttgtcacgtt aggatcaggg attttcccga tatgttcaat cacttctagt tcatttagag
     3781 taaatcctgc aaattctttt tccatttctg gtttattacg atgaataatc tcattagcta
     3841 aatccagata ttcttctatt agcagttgtc ttttatccat ttctacacct tctttttctt
     3901 tcattatatt gtttttttgt ttcctattca acaaagataa aaatattttg taggaaaacc
     3961 caatctgaaa ccaa
//
LOCUS       DAYYCQ010000150         3876 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000150 DAYYCQ010000000
VERSION     DAYYCQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.157"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(716..3865)
                     /locus_tag="ACHAYF_002858"
     CDS             complement(716..3865)
                     /locus_tag="ACHAYF_002858"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM8580206.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          635 g         1362 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttggacact tgaatctcgg ctgttttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cttccttcac gaatgggctg attaggaaac tcttttgtta
     2041 accagtcagg ttctttcgtc atttctttga tcgctcgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg
     2161 tataggcatt gtctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagt agggtccaaa taactttctt ttttgacagg ctccatttga tcaatcatac
     2281 gattgatctg ttccggtgaa tactctgcat atgtttctac ttcttttaat ttccgataga
     2341 ttttattctc taatgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaaa
     2401 ccgagccatc ttctaatgca ttcttgatgg tataggtatg caagacctct ccatactgat
     2461 catgggtcgt acgtgccaat tgccctttgg cttgtttccg attctcaggg aaaatcggtg
     2521 tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt
     2581 cagcacttaa cgctcgatga cactcatcta ctacaaacac gatatgttgc cccattaatt
     2641 tttggaagcg attggttcct tttttctctt cttgtttttt tgcatatttc aacgccgcat
     2701 ctagcttttg acgggttgta ataatcacaa catttgcatt ggcatctgcc aacagagttt
     2761 cacttaactc tttggcactt cctgttccca cgatcaagct attcgctttg gcgttcccgg
     2821 aagaaatccc agtattaaat tccgaagcaa atttcgtaaa ctctgtggtg gtttgattat
     2881 ctaaatcttt gcggtccact aacatgatcg tccgatcgat ccccgatttt cgtgctaaaa
     2941 gcttagtaga tacaaaacta gtcaacgttt ttcctgaacc agtcgcatgc cacacatatc
     3001 ctgattgatg tttatttgca gcaataaaca aggcttgaat cgcatgtact tgataaggat
     3061 gtaacaccat gaggatttta ttgtcttgat cttcactgac aatcgtataa tccgcaatca
     3121 agcgatgagc atccgggata tttagcactt gtttgcaaaa ctcatataag ttttctacct
     3181 ttttattatc tttcgttcgc caactaaata aaaacttagg atgcaaatcc tttggtaagg
     3241 catttgcaaa ataccgagtg gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa
     3301 agatattgtt ccgaaacata ccttcttctg catatttttt gatttggtta aacgcctgat
     3361 aaaaactatc ttttgcggtt acttgtttca gctcgatttg gacaatcggt aatccattga
     3421 tcagtaaggt gacgtcaaaa cgtcgatccc gtgcttctat gttacttcca cgttttgcaa
     3481 tttgatggac cacttcatag gtagagattc ctccaccaat gtcttgatta gaataaagaa
     3541 tgagcgagac agaacccagt tgcggatctt cccgttcaat cgtgatccga gccattccat
     3601 tttcaccttt taaccatttc gcagcatcaa agggggtttt cgttcgtagt aacaattctg
     3661 ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc attcagttct gcttgattat
     3721 ttgagatgat tttttggcgt aaattgaccc aaagatcttc ttctgatttt agatcgggac
     3781 gatacgtcca ctgattatgc ccttccccca atacacggat caactgttct tcgacttccg
     3841 cttcctcacg gtgactaatt ttcatttatc ttccct
//
LOCUS       DAYYCQ010000151         3836 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000151 DAYYCQ010000000
VERSION     DAYYCQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.158"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHAYF_002859"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHAYF_002859"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8580207.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACHAYF_002860"
     CDS             complement(2260..2511)
                     /locus_tag="ACHAYF_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8580208.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACHAYF_002861"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACHAYF_002861"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8580209.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACHAYF_002862"
     CDS             3461..3679
                     /locus_tag="ACHAYF_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580210.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1063 a          814 c          712 g         1247 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttct
//
LOCUS       DAYYCQ010000152         3775 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000152 DAYYCQ010000000
VERSION     DAYYCQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.159"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHAYF_002863"
     CDS             127..579
                     /locus_tag="ACHAYF_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580211.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHAYF_002864"
     CDS             593..1282
                     /locus_tag="ACHAYF_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580212.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHAYF_002865"
     CDS             1407..3758
                     /locus_tag="ACHAYF_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8580213.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1281 a          494 c          777 g         1223 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact attta
//
LOCUS       DAYYCQ010000153         3753 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000153 DAYYCQ010000000
VERSION     DAYYCQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.160"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHAYF_002866"
     CDS             <1..1087
                     /locus_tag="ACHAYF_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8580214.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACHAYF_002867"
     CDS             complement(1305..2447)
                     /locus_tag="ACHAYF_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8580215.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACHAYF_002868"
     CDS             complement(2452..2637)
                     /locus_tag="ACHAYF_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8580216.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACHAYF_002869"
     CDS             complement(2991..3236)
                     /locus_tag="ACHAYF_002869"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8580217.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACHAYF_002870"
     CDS             complement(3230..3631)
                     /locus_tag="ACHAYF_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8580218.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1238 a          679 c          567 g         1269 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tat
//
LOCUS       DAYYCQ010000154         3737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000154 DAYYCQ010000000
VERSION     DAYYCQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.161"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACHAYF_002871"
     CDS             72..155
                     /locus_tag="ACHAYF_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8580219.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHAYF_002872"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHAYF_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8580220.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACHAYF_002873"
     CDS             complement(1280..2143)
                     /locus_tag="ACHAYF_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8580221.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACHAYF_002874"
     CDS             complement(2145..2417)
                     /locus_tag="ACHAYF_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8580222.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACHAYF_002875"
     CDS             complement(2434..2643)
                     /locus_tag="ACHAYF_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8580223.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2742..3638)
                     /locus_tag="ACHAYF_002876"
     CDS             complement(2742..3638)
                     /locus_tag="ACHAYF_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8580224.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1148 a          654 c          579 g         1356 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt aatcacttgt gatttgtgat tctattcttt ttcattttct
     2761 aattgaatga ttcgctcaat catttcaaaa aatacgttct catacataga caaaactttt
     2821 ttgtcatagc ctttgtgttc tgtaatgcca tttttagacc aagtacttac tttattactt
     2881 cgcttgataa tatttcggaa aaccaaatta tcctctttat gttctttgta cagttcttcc
     2941 aggtttgatt ttatcgttgc gctgtccgta tcaactaaat aaggaacaaa accaatcata
     3001 tctagtccag ggttaaactg ttcttgcaaa tcaatcaaat aggaaatata gttttgaatg
     3061 ttgtttgtac tttcttcttc tgcttgtaaa gggatcataa cgtaatcact cgccacgatt
     3121 gcattatttg tataaacgct tggcgttggt acagtatcaa taataataag atcatagtca
     3181 ctttttaaag gtgctaaaag agtagcaagc aatctacttt cattttcaaa cgtccatgag
     3241 cgagttaatt ttggcagtaa catcaaatca aacgtgccag ggatcaagtc taaattatca
     3301 gtcaaatgaa taatagaaga agccaagttt ccatttttca agccttcata aaaattgaca
     3361 cgtggcaatt ctacctcaaa agtttttgct aagtcttttg tcaatgttgc ctgtaagtcc
     3421 ttatcgatca ttaaaacttt taaattcaat ttgtctgtca agtaagcaaa catagtcgat
     3481 aatttggact ttccaacacc acctttaaag taattattca agataataat tgcttcattt
     3541 ttattgttta aaatacgtct taattcttca agtattttta gtttttcctt ctccataact
     3601 acgccccatt ctttttttgt atagtaatat tgtatcatta agaatcacaa atcacaagtg
     3661 atttgtgatt aatcacttgt gatttgtgat aagtgatttg tgattaatat ataaaagccc
     3721 tctttaaagg gctttta
//
LOCUS       DAYYCQ010000155         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000155 DAYYCQ010000000
VERSION     DAYYCQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.162"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHAYF_002877"
     CDS             complement(<1..353)
                     /locus_tag="ACHAYF_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580225.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHAYF_002878"
     CDS             complement(613..1161)
                     /locus_tag="ACHAYF_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580226.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHAYF_002879"
     CDS             complement(1162..2019)
                     /locus_tag="ACHAYF_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8580227.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHAYF_002880"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHAYF_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHAYF_002881"
     CDS             complement(2581..2910)
                     /locus_tag="ACHAYF_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8580228.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHAYF_002882"
     CDS             complement(2987..3259)
                     /locus_tag="ACHAYF_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8580229.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHAYF_002883"
     CDS             complement(3259..3522)
                     /locus_tag="ACHAYF_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8580230.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAYYCQ010000156         3661 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000156 DAYYCQ010000000
VERSION     DAYYCQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.163"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1091)
                     /locus_tag="ACHAYF_002884"
     CDS             complement(387..1091)
                     /locus_tag="ACHAYF_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580231.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1278..1523)
                     /locus_tag="ACHAYF_002885"
     CDS             complement(1278..1523)
                     /locus_tag="ACHAYF_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580232.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1540..2391)
                     /locus_tag="ACHAYF_002886"
     CDS             complement(1540..2391)
                     /locus_tag="ACHAYF_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8580233.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2557..3561
                     /locus_tag="ACHAYF_002887"
     CDS             2557..3561
                     /locus_tag="ACHAYF_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8580234.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1141 a          623 c          708 g         1189 t
ORIGIN      
        1 tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga
       61 aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa cacaaaatat tatagaaccg
      121 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
      181 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      241 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      301 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      361 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      421 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      481 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      541 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      601 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      661 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      721 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      781 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      841 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      901 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      961 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
     1021 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
     1081 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1141 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1201 cgtattttcc cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat
     1261 aatccgttcc attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa
     1321 taaatgactt catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc
     1381 agttcttcga tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct
     1441 gcatcgatca aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa
     1501 ttcaagcgtt cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg
     1561 gtcttttgct ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt
     1621 taattcaaaa tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg
     1681 aagaggaagg aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc
     1741 atgtgcttca gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg
     1801 actatatgct tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa
     1861 ttcgcctggt gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag
     1921 tgtatctaga tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc
     1981 atacagttct tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa
     2041 agcaacaggg tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt
     2101 caatgattct tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt
     2161 tgttgtcaag aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct
     2221 ttcttcattc ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc
     2281 tttaacagag gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac
     2341 cgggatttcg taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc
     2401 tcctttttta ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt
     2461 gtttctactc ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt
     2521 ttttttccgt aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca
     2581 attctgcata ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat
     2641 ctgacaaaag aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct
     2701 caaacaaaaa ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct
     2761 caagaaaatt ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa
     2821 gaaagtgaag aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg
     2881 atgtacttat ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca
     2941 gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca
     3001 caaaaagcag atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa
     3061 aaaaaatatg agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt
     3121 gttcctgcac cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt
     3181 ggaaaaaaat ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat
     3241 gatacgatag aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag
     3301 gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca
     3361 gaagcacctc ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa
     3421 ttcaagctat caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat
     3481 ttgattgaat ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat
     3541 gaagtattga atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca
     3601 gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt
     3661 g
//
LOCUS       DAYYCQ010000157         3523 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000157 DAYYCQ010000000
VERSION     DAYYCQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.164"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHAYF_002888"
     CDS             <1..156
                     /locus_tag="ACHAYF_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8580235.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACHAYF_002889"
     CDS             175..1452
                     /locus_tag="ACHAYF_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8580236.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="ACHAYF_002890"
     CDS             1462..2118
                     /locus_tag="ACHAYF_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8580237.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="ACHAYF_002891"
     CDS             2118..3494
                     /locus_tag="ACHAYF_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8580238.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
BASE COUNT         1446 a          353 c          599 g         1125 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 gagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aat
//
LOCUS       DAYYCQ010000158         3490 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000158 DAYYCQ010000000
VERSION     DAYYCQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.165"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHAYF_002892"
     CDS             complement(<1..2232)
                     /locus_tag="ACHAYF_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8580239.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHAYF_002893"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHAYF_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          983 a          772 c          549 g         1186 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac
//
LOCUS       DAYYCQ010000159         3457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000159 DAYYCQ010000000
VERSION     DAYYCQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.166"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..>3457
                     /locus_tag="ACHAYF_002894"
     CDS             414..>3457
                     /locus_tag="ACHAYF_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8580240.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         1279 a          537 c          709 g          932 t
ORIGIN      
        1 gaaggtgatt tcaaattccg aagaaggaat ttgaagtcac ctttttactt atgtcacagt
       61 ctcattcagt aatgcgggat aaggctcctt attccgttat ttgaatactc tgaagtttaa
      121 tctaagaaag ggtaatattt tgaaaacatg cttcatcgca tagaaatgaa cttaatttca
      181 gaaagaatac atacatatct ttttatatgt tttcagctgc tgaatggtta aaaagcacaa
      241 atatccgaaa aaaacatgaa gaaatgtact ttttttgttg taatgttcat gaaattctct
      301 aagtaaatta gttaacataa ggttagtaag attcctattc tgtgaataat acgtatcaga
      361 tttatctttt attcttacta ggaaagtaat tattaaggga gttgatttga ataatggtta
      421 gcaagaataa taagagagta tttctagaaa aaacaaagaa acgagtattg aagtacagta
      481 tcaaaaaatt aagtgttggg gttgcatctg ttttgattgg tgttggtctt gtcttgggaa
      541 caactgaact agttaaagca caagatgaaa tttctccaag cagcccactt gaaacagcga
      601 taagctccgt acaagtaggt gacaaagtag cttcggggaa tacttttcag gagaatcctg
      661 gttatacgaa aaattataac ttttcggatt tacaatttag tcctcaagaa ctaactggag
      721 acacactaaa aggcaataca attggatttg aagtttacgg aaaacataat attgcagcat
      781 caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg attagctaaa tatgtagaaa
      841 aaattcaggt cgatccaaag aaaggaattg gatcttctag acggaccttt gtaagaatta
      901 atgattcgct gggtagacct acaaatattt ggaaggtcaa ttatattcgt gcaagtgatg
      961 gattatttgc tggggcagaa acaaccgata cgcaaaccgc tcctaatggg gtaattacgt
     1021 ttgaaaagag cctagatgaa atttttaaag agataggcat agacaatctc aagaccgatc
     1081 gtttaatgta tcgaatctac ttggtaagtc atcaagacga tgataaaatt gtaccgggta
     1141 ttgatagtac gggctacttt ttaacagatt cagatgattt ctataatagc ttagatgtat
     1201 ctgaaaataa tcctgaccag tttaaacatg gatcagtaag tgcaaaatat gagaaaccta
     1261 atactcaaac aaaagatggt tcaggatcca caggtgctaa tggagctata atcttagatc
     1321 ataagttaac gaaaaattat aatttctcat attcatcttc cgcaaagggc acgccttggt
     1381 atgctaatta caaaattgat gaacgattag ttccatatgt agcgggtata cagatgcaca
     1441 tggttcaggc tgacaaagta acatatgatg tttcgtttga atccggaaca aaagtagctg
     1501 atttagcaat cgagcgacgg gaaggtcatg aaaattatgg cataggttca ataactaata
     1561 atgatttaac taaacttatc gattttgcta atgcaagtcc acgtccagtc gttattcgat
     1621 atgttttaca attgactaag ccattagatg aaatcttaga agatatgaaa gcaacagcac
     1681 aagttgaaga aaataaacca tttggtgaag atttcatctt tgattcttgg ttgtcggata
     1741 cgaataaaaa attaatccag aacacttatg gaacaggtta ttattatttg caagatattg
     1801 atggtgatgg aaaccctgac gataaagaag agagcggaga cacgaatcca tatatcggga
     1861 aacctgaatt agaagaagta tatgatgttg acacaacagt taaggggaaa gtattcatcc
     1921 acgagttagc gggaacaggt cacaaagccc aacttgttga taaagaaggt actgtattag
     1981 cagaaaaaac tatcgctcca aatgaaaaag atggggctcc aatttcagat actgtagaat
     2041 ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa agatgaatta aaaatccaaa
     2101 tcgtttctcc aggttttgat aaaccagaag aaggttcaac cgttattaag gaatcaccaa
     2161 aagcggttga taaacaaacc gtggtagttg gatttaaacc agatgctaaa gaatcaattc
     2221 ggaataataa aaacttacct gaagatgcag agtattcatg gaaaacagag cctgatactt
     2281 ctaacgttac tgatagtacg aaaggtattg taactgttaa gatcggaaat cgaactttcg
     2341 acgtggatgt agagtttgct gtaaaagctt ctcaagctat ggaaaatgat gcaacatacg
     2401 tacctataac aacaactcca gaaacgacag ttcaaagtgg aaaacctaca tttgataaac
     2461 cagatgttcc tttagctaaa gatgcctttt caatttcaga tgtctataat aagtacttcg
     2521 gtaatgcaag tgttgatgca aacactggta ttgttacatt cactccagct aaaggtgtag
     2581 gagaatcgga gccgattact ggaacaattc ctattaaaat tgtttaccaa gatggttctg
     2641 taggcacgac cgatttagca gtaactgtaa gtaaagatat ttatgaaaat ccaggagaaa
     2701 acattcctgc aggctaccac aaagtaacct tcaccgcagg ggaaggaaca agtattgaaa
     2761 gtggaacaac agtctttgca gtgaaagacg gcgtaagctt gccagaagat aaacttccag
     2821 tgttgaaagc aaaagatggt tatacagatg cgaaatggcc agaagaagca actcaaccaa
     2881 ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa actggatgat atcattgaaa
     2941 atccaggaga aaacattcct gcaggctacc acaaagtaac cttcaccgca ggagaaggaa
     3001 caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc ttaccagaag
     3061 ataaacttcc agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg ccagaggaag
     3121 caactcaacc aattacagca gatgatacag aatttgtatc aagtgcaaca aaactggatg
     3181 ataaatctga cgctgacaaa tataatcctg aaggtcaaaa agtgactaca gaattgaata
     3241 aggaaccaga cgcatttgag ggaattaaga acaaagaaga tttaccaaaa gatactaagt
     3301 atacttggaa agaaaaagta gatgttagcg cagctggaaa taaaaaaggg actgttgtag
     3361 tgacatatcc agatggatca tctgatgaag ttgaagtaga tgttacagtg acagacaatc
     3421 gttctgacgc cgataaatat gagccaacag tagaagg
//
LOCUS       DAYYCQ010000160         3362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000160 DAYYCQ010000000
VERSION     DAYYCQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.167"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(579..1193)
                     /locus_tag="ACHAYF_002895"
     CDS             complement(579..1193)
                     /locus_tag="ACHAYF_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8580241.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1799..2176)
                     /locus_tag="ACHAYF_002896"
     CDS             complement(1799..2176)
                     /locus_tag="ACHAYF_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580242.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2476..3357)
                     /gene="galU"
                     /locus_tag="ACHAYF_002897"
     CDS             complement(2476..3357)
                     /gene="galU"
                     /locus_tag="ACHAYF_002897"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8580243.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1028 a          680 c          507 g         1147 t
ORIGIN      
        1 tttccttaaa attctttttt tatttttaga ttagagaaaa tctcgtcctg tttttttcat
       61 tttacaatca ttgaaaaatt gaatttcatt gcaaaaaaaa taataaacac ttataaatat
      121 ccattatcta tccttcaatt tttgtgtatt ttatttctat tagaaaaagg tatcctgttg
      181 gacaattttg ttatatcagg taaacttcat tgcaataatg ataacaaatg tactataatt
      241 actttgtaaa gagttttggg gaagactctt tcatcaacgt gtattaagag acaagaaaga
      301 atccatatgg attctttctt gtctcttttt ccactacttt ttatttttct gacactacat
      361 attcttataa ctcatttctc ataactgtga aacaacttat aaaaataaca aactttaaac
      421 aaaacaagct taaaatcaaa gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc
      481 gttgttaatc ctcattaata atcgttttct ttttttcaaa cgctccattt ttatgtggaa
      541 cattattttt ttaaggaact ttgtcaacat gtggataact attttccact tgtgtcctgg
      601 actagctgtt ttgctgtaaa atacaaaact tgctgcagca aaaagatcgt tgcaagagag
      661 gctgttccgc agatcgctgg atctagattt ggcaaagtca agactgcatc atgtaaattg
      721 gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc gcaaaccact gctatgttgc
      781 gatttgatac ttcgtgacaa atcaaatcca gtaacaatga ttaatggatg accattttct
      841 ttgatccact gagctagttc gatgattgcc gggtttcttc cttcattcga aagaagaaga
      901 aaaatgtctt ctgatttgac agaaagctga tcaataattg cctgtcctgc accttctagc
      961 gtttcataga cacctccggc tggattttca atcaatatac ttggtacaag atccggggtt
     1021 tgatcccaaa attcaaatgc tacaccactt agtgtgcggc tagcatatat ctgcaatcta
     1081 ccaccttgat taatttgttt cgcgatccaa gtgccagctt gaagcatcag cggctcttct
     1141 atccttaatt tctcttccaa ttggtgataa tagtcaaaat aacataaact catttatctc
     1201 tcccgcctag ctatacttga catgaataga aacagctgcc taatctaagg aagtacataa
     1261 aaaaacagta gttagacagt aacacatgtg gtctttgctg tttcaaacac aaaaaaggca
     1321 ttgaacgaat acgcccaata ccttgacata gcaaataaaa caacgtgcag accatggaaa
     1381 atatctacat aggggtagaa ttttcaccct agattaggta caataaatta ggtcgatgca
     1441 ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt gtgtgactgg tcattccctg
     1501 attcttagcg ccaactaagg gtcagggagc ctttttttat tcagctgttt ctatctgaat
     1561 ttattgtaag taatctctta tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg
     1621 atttatatat atataaataa cataaaaaaa ggaatggctt atcccgatac aacaagcatt
     1681 cctttaaaaa agattctttt atataagtga aaaaagcttt tttccaccaa ttaaataaat
     1741 ctctcatctc aatagtttat ccattatttt atcttttttt gcgtatacct actaaatatt
     1801 accgtcttct tctcgttctt ctactttgtt cgagagaatc tgtactagaa aaagtagttg
     1861 tatgtctacc ttttctgcct atccgagact ttcttttcat atatgggata taaatgatta
     1921 gatacagtac agcaaaaaat aaagcaaata cgcaagcttc ccaactacct aacgccaatt
     1981 caaccatcaa ggtagcaacc ccaacggatg ctgcatacca gataaaaatc agaaacgttt
     2041 ctttttttct gaccatgcga aacatgataa atagactaat aagcaatagt ggcaacacaa
     2101 atatccaatt gcttccttcc atcagccact tgagtatatc aagcagcgac tgtgcaacag
     2161 cgactaattt tcccatagtg aacacacacc tttttttata attaaaaaat tcgccttttt
     2221 attctactat gaatcttctg gcttggcttt catttctctt atgtaatcat tttcttttat
     2281 acaacttaaa aaaaaattaa accattagca taatattttc tgaatctatt cacgaaagtc
     2341 tttttctatc tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca
     2401 gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa tagcctttca ttctcagcag
     2461 gccacttatt tccagttaac tttctgttct tagcttttta ctcaactgga taatatatgc
     2521 acttaatgaa gtcccaattt ccgaatgttg aagtccatat tcaatatttg tcttcatata
     2581 tcctaactta tcgcctacgt cgtatctttt tcctttaaac tctctcgcat agaccgcctc
     2641 tgtctggtta agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc
     2701 ttgagtttct aatatctcaa atattttagg agtcagtaga taacgtccga taatggctaa
     2761 attacttggt gcttttccca tttcaggttt ttctacaaac ccttttactc tgcaacgacc
     2821 cttttcgcta tctgttttag gatcaataat tccgtattta gatgtttcat tttctggtac
     2881 acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa tccatcatca attgttgagt
     2941 caatgggata tcatctttca tcaaatcatc ccccagcatg acgacaaacg gttcatttcc
     3001 tataaatgct tttgcttgaa gcacggcatc ccccagtcct cttggatgag attgacggat
     3061 gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg agtaattctg ttttttcctt
     3121 ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag tgatcttcaa tgggtctttt
     3181 ttcttttccc gttacgatca agatgtcttc gattcccgaa tccaatgctt cttccacaat
     3241 aaactggatc gttggtttat ccacgattgg aagcatttct tttgccatgg cttttgttgc
     3301 cggcaaaaaa cgtgttccta gcccagcggc aggaatgact gcttttttga ccttcacttg
     3361 ct
//
LOCUS       DAYYCQ010000161         3011 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000161 DAYYCQ010000000
VERSION     DAYYCQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.168"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..2240
                     /locus_tag="ACHAYF_002898"
     CDS             180..2240
                     /locus_tag="ACHAYF_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8580244.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2323..2892
                     /locus_tag="ACHAYF_002899"
     CDS             2323..2892
                     /locus_tag="ACHAYF_002899"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8580245.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1093 a          538 c          567 g          813 t
ORIGIN      
        1 attacttaac aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa
       61 aaatcaaaaa aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa
      121 atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa
      181 tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac
      241 ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat
      301 ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac
      361 gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg
      421 caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa
      481 cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca
      541 gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac
      601 agatcactga tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag
      661 tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg
      721 cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac
      781 aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag
      841 gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat
      901 ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa
      961 gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa
     1021 cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag
     1081 aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag
     1141 ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata
     1201 gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg
     1261 atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg
     1321 aacaaaatgg gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa
     1381 aaagtcactg gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc
     1441 aatcaggagg ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag
     1501 gtcatgatct tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag
     1561 caacaaattc cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag
     1621 taccattgga ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt
     1681 atcatgaccc gtattcactc caagaactcc atgctcagtc actattacat gttttaccat
     1741 taaacaaaca gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa
     1801 aacaacaaat ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag
     1861 agttagcaag aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc
     1921 atttggcccc acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac
     1981 aatcagaaaa agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga
     2041 aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc
     2101 aagtaccact tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt
     2161 taggagattt catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg
     2221 acgcagatga ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt
     2281 catatgttat ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta
     2341 ccttacgcag aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta
     2401 gcggaaggtt ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt
     2461 gagacttccg ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac
     2521 tttgctcctt atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga
     2581 gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt
     2641 tttcattggt tagcagtgaa atcagattac caaggaaaag ggctcgcaaa agcattgact
     2701 gccagagtct tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca
     2761 cagacatgga gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct
     2821 gaaaatctag acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa
     2881 ttagataaat aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt
     2941 cattcctcat atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac
     3001 attcaaaaaa g
//
LOCUS       DAYYCQ010000162         2926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000162 DAYYCQ010000000
VERSION     DAYYCQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.169"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..188
                     /locus_tag="ACHAYF_002900"
     CDS             <1..188
                     /locus_tag="ACHAYF_002900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580246.1"
                     /translation="FMNGVVTKALSQLLFLNKRCARDSLSAIRHDFVKYGKRFFKIAS
                     YRSRLNISVTTSPLNGV"
     gene            482..2575
                     /locus_tag="ACHAYF_002901"
     CDS             482..2575
                     /locus_tag="ACHAYF_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8580247.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDKDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            2600..2902
                     /locus_tag="ACHAYF_002902"
     CDS             2600..2902
                     /locus_tag="ACHAYF_002902"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8580248.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         1015 a          498 c          586 g          827 t
ORIGIN      
        1 tatttatgaa cggagttgtg acaaaagcct tgtcgcagct cctttttctg aataaacggt
       61 gtgccagaga tagcctatcg gcaataaggc acgattttgt aaaatatggg aagcgatttt
      121 tcaaaatcgc ttcttatcgc tcaaggctga acatctctgt cacaacctct ccactaaatg
      181 gtgtttaaaa gcttgcatct gcgataataa gaacaaccaa acgaaaaata ggaagcacta
      241 ttttagttta gttgttctta ttacttgatg caggacagct ttttcacaac ctcttttttg
      301 tatagatggg caaaattttt gtaataatag gcgtatgtct ttttcgttga ggagatgaaa
      361 aagcatatgg cttatttttt gtttgctttg cttactttct ttccacttca agtggcagaa
      421 atatctactt tttattatgt aagcggaacc atatacttga aatcagaagg agatcatttc
      481 tatgaaaaca agaacaattg aaatacttca cagaatcatg acagcccaat atccagtaaa
      541 agcaaaacat ctagcaagtg agttcaatgt cagtcagcgg acgatccgtc aggaagttct
      601 tgaagcgaat atctggttga gcagtaaaaa gcttccggag atccgtacaa ttcgcaacaa
      661 aggttttttg ttgaagctgg aaaaggaaaa gcaagctcga ttagaaactg aaataaaaga
      721 tgtgaaagac ggctttttga atcggcgcga acgtattttt gatctagttt tagccgttgc
      781 ttacagccga atcccgatcc atctaaacaa aaaagaagaa gaatttcaaa tctctaaaag
      841 tacattagat aaagatatga gacggttacg agcaacactg gtcagttatg ggatagagat
      901 cgtcagttat ggaaagcaag gactggttta taaaggttca gaacgatcta tccgtacgat
      961 gatctatgat tttatcaatc agaatctggg aatgaccgac ttctttatga atcaagatgc
     1021 acaattaaca ccggctcaaa aaatctttct ccattatttt cctttggtgg aaatcaaaaa
     1081 aatacaggag atctattcca ataagttgtt gaaacaagaa gatgatattt ataaaaaaca
     1141 aatcctgctt tttacattga tctggatcca gcgaatcagg aaacatgaat ttatctctgc
     1201 cattaattgg aaaaatacag aaataaaaga cagtagcttt tctgcattta ttgaccacat
     1261 gatcgaggaa tttcaattgg acaatgtccc gcaagtagaa agaaattatc ttcgtttcac
     1321 tattgagacg ttcaatgcta gagatatctc caattcaatc gagtgggtac aagctcaatt
     1381 actaacgatc caattgatcc aatttgtaga gaacgagaca cacgtacctt tccatctaaa
     1441 ggaagaagct ttattcgaaa atctctataa gcacatggct gcactgattg taagaatcaa
     1501 gaatcatatc caagtcatga atccattgaa agaaaacatc cgaacaaatt atttcccaat
     1561 ctaccgcgca gtcactcatt ttgttcctac gatcgaagaa gtaattggtg gcaagatact
     1621 ggaggacgag atagcttttc ttgtcatcca cttttcgacg atcgccagtg taatcaagcg
     1681 agacttgaat tatgtttata aatcagtcgt tgtctgtaac catgggatgg ctactggaaa
     1741 tctgttagca gagaatctga aagaaaagtt tcctcagatc gagattactg cagttttaag
     1801 ttcaaaagaa atcgaattag tagataaatt ggatgtcaat ctggttttca gcacctttca
     1861 attgaattat cgaaataagc cgcttttagt tgtcgatccg attttaactg agacgaatat
     1921 cttggttata gaagactttt tgaaaagaaa tgaagaacta cagcggttag agcctaacag
     1981 tcatgaatcg acggagttat tcactcaaat aatagatgtc atgaaagaaa gtggtggccg
     2041 agtagatcga atcatctatg gcaagttaga aaaattattc gaaacaaaca atttagaaat
     2101 caacaaaagg gagatccagc ctatgttaaa agatattttg acggacagca atatcatgat
     2161 caaagaaaaa gccactactt gggaggaatc gattgaacta gctgctagac cattggttaa
     2221 agaaaacatt gtcgaagagc gctatgtcga agcgatgatt cgtgcagtga atgattacgg
     2281 accgtatatc gtgattggca aacacatggc actagcccat gcccgaccag aagatggggt
     2341 caataaatta ggagtcagcg tagctacgat tgagcaacca atcgatttcg gtaatcctga
     2401 aatggatcca gtaaagatta ttttttgttt ggcagcagta gactcctact cgcatctgaa
     2461 catcatgaaa gaactaattg aattgatcaa caacgaagag aagttggatc gattgattga
     2521 atgcacaaat atcgaagatt tcaaacaact gcttttttca gtagttgaga aataataaat
     2581 gatacggggg agaacaaaga tgaacaaatt gaacatttta ttcgtatgcg gtgcaggttt
     2641 aggaagtagt tttgcagcac agatgtctgc tgaggatgtt ttgaacaaat taggagtaga
     2701 agccaaactg gaccattgtg atatttcttc cgcagtttcc atgaaaccag atgtgatcat
     2761 cacagcagaa aattttcgtt cgcagttcga aaagttcgca gttgatccta aaacggcgat
     2821 gatctatttg aaaaatattg tttcaaaaaa tgaaattgaa gaaaaaatca ccccagttct
     2881 gcaagataag ggagttatct aaatacaaaa agaaaagagg gtaaaa
//
LOCUS       DAYYCQ010000163         2772 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000163 DAYYCQ010000000
VERSION     DAYYCQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.170"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..224
                     /locus_tag="ACHAYF_002903"
     CDS             <1..224
                     /locus_tag="ACHAYF_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580249.1"
                     /translation="RTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDVSTS
                     NSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(315..680)
                     /locus_tag="ACHAYF_002904"
     CDS             complement(315..680)
                     /locus_tag="ACHAYF_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580250.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            888..1370
                     /locus_tag="ACHAYF_002905"
     CDS             888..1370
                     /locus_tag="ACHAYF_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580251.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1567..2457
                     /locus_tag="ACHAYF_002906"
     CDS             1567..2457
                     /locus_tag="ACHAYF_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8580252.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          945 a          446 c          540 g          841 t
ORIGIN      
        1 ttagaactaa acgaagtttg tcttctaaaa aaaattgggt atactcacaa actatttttt
       61 atggaggaat tgttttaatt agtttacttt cttcaacact ttattctttg aatattatag
      121 atgtttccac ctcaaattca atatctatta ttggaatcat tattgctgca attattacac
      181 aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa atagaaaaac tagtgatagt
      241 tttggtgata taagaaaaac atatatgtat aaaaagaaga aagtagcttc tctttgtaca
      301 tatatggttt gtttttatgg atgccgagct tttttataag ataaggctac cgcccataac
      361 gacccaagga tttgtggaac taagtaaata cctgcaagaa gcaagatcag ctgggtcggt
      421 tttaaaggat gatagaagta acgccattca gtaaaattgg cgcaaaataa ataggaagtg
      481 acagtagata taatattggc aatgaataaa gatataatac gcctgttcct ttgaaaataa
      541 tatccagcaa aaagagataa aaatatcaat gtgatgaacc cgcttacgga atgataacga
      601 aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag ataaaagcaa cggaaggttt
      661 ttccagcaag tttttatcat ctttcatcac cacctaacaa gtgttattat tttgttagtt
      721 ttattgtaaa ttgaatcctg caataatcaa ttactttttc attatttcta gattaaatga
      781 ataaactctc atgccattat ctctaaaaaa aaagtttttc cccctaattt ttttagatag
      841 aaaattgtat agtatgatga gagaaaaagt agtttggggg aacgttaatg agaagaaaag
      901 cagtaatcga tcggctttat ttattttttt ggttttttcc tatcgttttg ctacctttta
      961 catgggtaaa tgctgttgta caaggtgcgg agatattagg cctgatccaa ggaagcggat
     1021 ttgtggtttc taggaatttt ccagctttga ttgttttcta tttagtgata agtgtaggtc
     1081 tcttattacc aaaatctctg atcaatcact acaattggca gctgctttac ttttcttgct
     1141 tattagtatt tacatctacg ttttctttgc tgcctcgctt gattatcggc cctaatggtt
     1201 tgagcgagag tggattgaag gatttagctt attcctccat gtatttcttg acgatcaaac
     1261 catggttcta tttagtaatt gcctcattta ttcttgcaac atttttttac ttctggacag
     1321 aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa taactattga tggtggatca
     1381 tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc tcatatgctt tttttattat
     1441 tatgtaaaga atgcgaaaaa cgtttacatg atttttacat aacttaacta tatttttaca
     1501 gcaaccatac gagggattta cagtcgactg ataaacttta gttgtaaata agaaaggagc
     1561 aataaaatga aaaaagtatt gtttactcta ggtatgatgg gaattctttt agccggatgc
     1621 ggctcaggat caggaaatac agatggctct gccacacaaa ataattcaag tgactcaaat
     1681 gaacaagtaa aaatcgttgc ggtaggttcg acagcattac agccgttagt cgatgcagca
     1741 caagaaagct ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg aggaggaagc
     1801 ggaactggtt tgagccaagt cgaagctgga gcagttacta tcggaaactc tgatgtattt
     1861 gcagaagaaa gagacggagt tgatgcttca aaattagtgg atcataaagt agcagtggtc
     1921 gggatggcac caatcgtcaa taaagataca gatgtcaaag atatcaccaa acaggaattg
     1981 attgatatct ttacaggaaa aatcactaac tggaaggaag ttggcgggaa agatcaaaaa
     2041 atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga aaaatgggga
     2101 ctggatggtg ctacccctgt gcagtcccaa gaacaagatt catcaggaac agttcggcag
     2161 ctggtaagtc aaacaccagg agcaatcagc tatttagctt tctcatatct cgatgattcc
     2221 acacaagcac taagtatcga cggtgtagaa ccaaaagaag agaatgtagc agataacagc
     2281 tggggaatct ggtcatatga acatatgtac acgaacggaa aaccttcccc tgaagtccaa
     2341 aaattcttag actacatgat gacagaagaa atccaagaag gacctgtcaa agaattgggc
     2401 tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat caaataacta
     2461 gcagataaac atgccacggt agtcgttact attaaggaaa aatcaactat gtttccccac
     2521 atatacattt ttcctttaat tgtgaaaaat agttcctcat attttgccgt tttttgagct
     2581 aataccgagg gtcagctgat tgaacaccgt ttattcgaaa aaaagagcta cgacatagaa
     2641 ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg tgaagacaaa tttgaataaa
     2701 acttagaccg atatgcggcg cgttctgtgc gccgcatatt tttttagttt tcaaagatca
     2761 gaccttgttg ta
//
LOCUS       DAYYCQ010000164         2768 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000164 DAYYCQ010000000
VERSION     DAYYCQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.172"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1874)
                     /locus_tag="ACHAYF_002907"
     CDS             complement(75..1874)
                     /locus_tag="ACHAYF_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8580253.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(1998..2594)
                     /locus_tag="ACHAYF_002908"
     CDS             complement(1998..2594)
                     /locus_tag="ACHAYF_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8580254.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          976 a          522 c          480 g          790 t
ORIGIN      
        1 aaaacgcata tacctgcggt ggcaggttat gcgttttttg ttgtataaag acttttttca
       61 ttaacgattt ttgattagcc gactcccaaa acaaaattta gcagtttatc tgaataagtg
      121 atttcttcat ctgtttgttt cacactttca tttagtagtc ccatctcatc ggttatgata
      181 ccatccacac cataaaacat catgcgactc atagcatctg aatcgttgat tgtccataca
      241 tatacttctt ttccgtcttg gtgggcagaa ttaatgaagt tacggttgat cgtactatat
      301 tccattgtca gaaaatcagc aggcgtcaca gggggcccaa caatattgaa aggtaagatg
      361 tagcccacat aaaagtttgg ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa
      421 ctttgcaaaa tatgatggtt ctctaagata actttgcggt atttatggac aaatcgttcg
      481 acaaggtcat ccgaatcttt tttagaggtc ttgatctcaa taagtaattt ttggtttagc
      541 ttatgtgctt tttccaaata agaatcaaaa gaacaaacag gggcttgcac tccgttttca
      601 gtaactgtaa gatttgtcaa ttctttcaat gtaagatcct gtggtgttcc atttacccct
      661 gttaagtgac gaagatttgc atcatgcatc actacgaaat gaccatcttt tgtttcctga
      721 acatccatct ctacataatc aggatgatag tctctgcttg ttttttcaag tgcttctaaa
      781 gtattttgcg ccccattttg ggtactcaca cctctgtgag agacagtcac tggttttgtc
      841 tgaacagctg aagtcagata atccatattg taaagacaga cgccaatacc gaaaaaaact
      901 gcaaaaagag tcaaaccagt gtttttcagc gcagagaaac gcaagttggg ggcttgtggg
      961 ataaaccact tagggatctc aggcaagaag ccttcgtcat ccatgaaatc aacaatgata
     1021 taaaaaatcc ctacggtaga catcacgata ttgaataata aaatcccttg taaaagcgtc
     1081 atagcaaaca cagcgctgat caatgcatag tctgggaaaa attgttcaac catactttgt
     1141 gccaaaataa caacgatata tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca
     1201 ataaattggc ccgtgatggc caaaagacgc gatttggtca atgaccagct ttcccgaatg
     1261 gctgctcgaa aaggacgatc tcgtagaatc atttccggta aagcaaaaat cagtcgaatc
     1321 ccaatatagc caaggaaaat ataaacgagt aaaaaggaac tgacgatgat ccaacgatta
     1381 gcaaaaataa aatccataat gaaagcgggg atttttattt ttgacagtag atcagaatta
     1441 aaacttaatc cgctgatcgg tagaatcaat aaaaaataag caaggaaaaa caaaaaagta
     1501 atgggtctca ctttcttcag ctgaagtatc gtcaagtgta agagttgctt caaagagatt
     1561 ggttctttct ttttaataaa aaaaacactc atcaaaagaa aagtaaattc gaaataaact
     1621 aacagtaaaa tcgtcatcaa aactacgatt aacgagagta gaaccccagg atgttgagaa
     1681 aaaattgaag gaatattgtc ataagacaaa tagtcaattg cccctcggtg taaaataaaa
     1741 cgggtcgtac tgctcaataa gggtaaacaa ataaataaaa taaagccatg catcagcaag
     1801 acatcacgga aataagcttg tgttcctttt aaaaagtccc aagcattacc taagctacgt
     1861 ttcaaataat tcatcctatc acctgctctt cacattgtag cgcaaatcta atgaaaaagg
     1921 aaagggtgca gtgtgttttg cagacccttt cccagattta acattgtcaa tcaatgacta
     1981 attttacaaa ataaatatta tccaagaaga ccacttagat attgacctcc ataaatcaag
     2041 gccatactat ataagaaaat cgacagtggt ttagcaatca aaatgattag tgtatatcgt
     2101 ttcactgaca tgtttgtcaa tccagccatc aaacataacg catcgtcagg agcaactgga
     2161 agaaaaatag ctagagcaaa cagcttctca aaacggtttt gattatccag ccagccaata
     2221 tatttgttat atgtcttatc gctaatcatg gaaagtatga atggctttcc ataacgacgc
     2281 cctagcagaa aaatgataat cgaaccaatg cagattccaa tatagttata gataaatcca
     2341 gcataagggc caaatatcag gacaccagct gcagtactga tccctccagg aatgattgga
     2401 ataactactt gcaggatttg gatgaagaca aaaataatcg gtcctaaaat gatggaatca
     2461 cctactaatc ctcttaacgc attcaaatct ttgaaaacac ctaaattaat aaaataaatc
     2521 gtcagcgcga ttgataggcc taatccgata atggatatga aatttatcag ttttcgagag
     2581 gcagcaatat tcattattca tcaactcctt agaggttctt tgttaactat actttactag
     2641 aaaagcaact aactgcgcca ttttttgata tgacagaaaa caaacaattt tgtaagttca
     2701 tcataagtat aaaagattga tctcaaaaaa acatccaact aaagacttat ttaataactt
     2761 gttttttt
//
LOCUS       DAYYCQ010000165         2674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000165 DAYYCQ010000000
VERSION     DAYYCQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.173"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACHAYF_002909"
     CDS             699..785
                     /locus_tag="ACHAYF_002909"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8580255.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACHAYF_002910"
     CDS             complement(1087..1311)
                     /locus_tag="ACHAYF_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580256.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACHAYF_002911"
     CDS             1677..1961
                     /locus_tag="ACHAYF_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580257.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACHAYF_002912"
     CDS             1964..2380
                     /locus_tag="ACHAYF_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580258.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2674)
                     /locus_tag="ACHAYF_002913"
     CDS             complement(2517..>2674)
                     /locus_tag="ACHAYF_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8580259.1"
                     /translation="PALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT          882 a          497 c          430 g          865 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggat
//
LOCUS       DAYYCQ010000166         2620 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000166 DAYYCQ010000000
VERSION     DAYYCQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.174"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..313
                     /locus_tag="ACHAYF_002914"
     CDS             8..313
                     /locus_tag="ACHAYF_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580260.1"
                     /translation="MPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLV
                     MMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
     gene            477..731
                     /locus_tag="ACHAYF_002915"
     CDS             477..731
                     /locus_tag="ACHAYF_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580261.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            801..1046
                     /locus_tag="ACHAYF_002916"
     CDS             801..1046
                     /locus_tag="ACHAYF_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8580262.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1122..1487
                     /locus_tag="ACHAYF_002917"
     CDS             1122..1487
                     /locus_tag="ACHAYF_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8580263.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1548..2111)
                     /locus_tag="ACHAYF_002918"
     CDS             complement(1548..2111)
                     /locus_tag="ACHAYF_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580264.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          770 a          470 c          415 g          965 t
ORIGIN      
        1 gattattttg ccatttattg cgattttatt cgatttagtt gcggcagcag cttattttct
       61 ccaattaaat tatcaaacct caggaattct gtttattgga atgatttttc aaggtgtgat
      121 tacccttttg cttcttgtca tgatgctcag ctataaagga aaaagatatg cccgtattca
      181 aacaaaagta tttgttaaat atgtttccat tcgctatggg atcatcattc tttccttttt
      241 agtgaatgcg atcgtcctct ttttatatgt attgaattac ttagatatca atccattgat
      301 tttttctcga tagataagac acattcagcg gaacagttcc tgcttatcag cagaaaactg
      361 ttctgctttt tcatttttca ttgatctatt cttcttttat tttcctagca tttcatttgt
      421 gatgtatgca ataacatgtt atcttttatt atatgaatcg ttgaggagtc cgttacatga
      481 ttacgttggt gagagttttg ttttgggtgc ctgcggtcgc actggtcgca agtattgttt
      541 atcttatgaa ttggaacaaa gaacggtttt atttagctat tttgactctt ccggctatct
      601 actttatgtg gaaagttttt aattacaact actttgaacc cgatagtgtt tttatagaag
      661 aattatcagg actggttttg tctttattga tcgtcattct ttatttgata cgtttaaata
      721 aaaaacattg agtactattg attgcatgtg tagctttcta tagtcattat aactattcta
      781 ctataatgaa aggtgattaa atgagtatcg aagaagctat tttactggat actttacgtt
      841 gtcgtctgaa tttcttaaga actggccgat ctttatctca agtagctgtt tctagcgcgt
      901 taagtattcc tttattcaaa tatcgtaagt acgaagaagg agaagaaatt ccaagcatca
      961 ttgaattaat ctatatcgct gacttttacg atatttccgt tgattaccta attggtcgct
     1021 cagaaacagc aagcaaactg aagtgatttt ttccagcctt ggtgctggtt ttttcattta
     1081 acgtctaagg ttaacaatct atatataaag gagacatttc tatgcgatta tggcatgaaa
     1141 aattgatttc aaagcttcct cgtcaacagc ttttaggaca acatcgggaa tgctgtgcat
     1201 taagaggaaa tggctgggga aagaaacatg ctactgtgga ttatgttttt cactattctc
     1261 cttatagatt attccaatat catcagcttg tcatggatga aatggaaaaa cgtggttaca
     1321 gcccagcacc agagtggaaa aatcctatgt atcgtggcaa atcatgcgaa gcattttttg
     1381 atttacctac tgtagagcgc aacgtgccga tttatccgga acacgatgaa gtttatctag
     1441 cagaatgttt agcaaatttg gagcaaaaag gaatttatct tacctaatat aactagaaaa
     1501 gccttctatc tcctcttact tcattaaaaa gctttaacca tcttagatca agctaaaatg
     1561 tcgtaagttt tagggttatc ttcatcctca cgtgcaaaac taaaagcgta ctctagtttt
     1621 ggttcatcgc caacttccct agacataaat gaacatctcc tgacgtggtt taaaagtacc
     1681 gcatcttcga cgtctgtata cgatgcacag cacagattgc ttcttttgat ctttcttttg
     1741 atttttctgt tgcttcttct acaccttctt cgattactac atcgatgctt acaattgctt
     1801 ctgcctaata ttcccatgca tcgtcgattt aagcctctgt agtcactgtg atcgtttcca
     1861 cattttaact cctctgtcgt ggcttcagtt gattttgtga tcagttcagt tgctaagcca
     1921 gagtcagaac aacctgccat tgctagcgtt aatattgata gtaaaaatag tttttttatc
     1981 ataaggttcc ccccctgaaa atagactaaa ataataacgc tttctttttt tgttttcaga
     2041 cgaaaattaa taatttctaa atattcttta aaaaagctta taactattat gcctttttta
     2101 atatataaca cagttatttt taaaaataag aatgctaaat cactctacaa taacggcgaa
     2161 agcacaaaaa ataaaaaaat tattacgtta ttaaaaaaac agatgaaaga tcaggtttta
     2221 ttagatctca ttctttcatc acttgaaggg atttaatgaa ctactttttc ctctttgtcg
     2281 tttttatata ttcgtattcc ttcaaacagt aggatagcct ctttcaatgc ttgatatttg
     2341 aactgtccgt tacttaaaaa tttagatgtg ccgccctctt cctagaacat attctttttt
     2401 actttttcat taattgacag accaatcgaa cgaaactgta aatttcgata ggataatgat
     2461 tggtcaaact cattcatttt caagacatct cgattcatat tcaagatagg aatcttttta
     2521 tcaacatcac tttattcgta ttttcctcta ccataaaagc ttcacgctcc acaaaattgt
     2581 tttatacact actattcagc ccattcaacg accaatgaat
//
LOCUS       DAYYCQ010000167         2595 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000167 DAYYCQ010000000
VERSION     DAYYCQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.175"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACHAYF_002919"
     CDS             <1..1412
                     /locus_tag="ACHAYF_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8580265.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2595
                     /locus_tag="ACHAYF_002920"
     CDS             1546..>2595
                     /locus_tag="ACHAYF_002920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8580266.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLL"
BASE COUNT         1019 a          363 c          520 g          693 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tatta
//
LOCUS       DAYYCQ010000168         2536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000168 DAYYCQ010000000
VERSION     DAYYCQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.176"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="ACHAYF_002921"
     CDS             complement(<1..1097)
                     /locus_tag="ACHAYF_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8580267.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            1327..>2536
                     /locus_tag="ACHAYF_002922"
     CDS             1327..>2536
                     /locus_tag="ACHAYF_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8580268.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVL"
BASE COUNT          768 a          519 c          471 g          778 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttgggctct atactttttg atgttggtga acttcaccaa catctttttt gtataaaaaa
     1261 atatagaaaa gacaccctga aatcctttag aattaatttg gctaccaatt cataggagga
     1321 ttcaagatgt ctcatactca gcttatcaaa gataccctaa atattctaga cctaaatatc
     1381 tatttcgagg aaaattgttt aactaaagaa aaatacaaag ggcaaatttg cttgatttac
     1441 aaaggaactc ttctgtacaa gccagaagag tgttcccatt gtctttgtgt cgtcccctct
     1501 aggatcattc gttgggggac gacaaccgtt cggttgttac tgaatgatgt gtctgagtat
     1561 cgcacttatc tggagctgaa gaaacaacga tttaaatgta agtcttgcca aagaacattc
     1621 gtagcggata cctctgttgc tgaaaaacac tgtttcatta gtcaaaaagt tcgttggtct
     1681 gtcattgctc gcttaaaaga gaatacttcc atgacggaaa tcgctcgtca gaaaaatatt
     1741 tccacttctt cggtctatcg tgtcatgaag cgattctacc gcccattgaa tcctttcaaa
     1801 cagactttgc caaaggtctt atgctttgat gaattcaaat cggttcgaag tgttgccagt
     1861 gcaatgagtt ttatcatgat ggacggacaa agccatgcat tattggatat tgtcgaaaat
     1921 cgacgcttgc cttatttaga acgctatttt tctcggtttt cattagcgac tcgtgaagcc
     1981 gttcaattga ttgttattga tatgtatgcg ccttatgttt cgttggtgaa aaagcttttc
     2041 ccgaatgctc agttgatcat cgatcgtttc cacatcgtcc aacatattgg cagaactttc
     2101 ttgaatcatc gagtgaaaga aaccacggta cgacttaaag gaaattccca ctctcaacga
     2161 gggttaggga aaaaattgaa gcgctattgg aaactcctac aaaaagaaga agcaaagtta
     2221 aactatgaaa aacgggtttg gcgtgccagt tttaaagatt atttgactga aagtgagatt
     2281 gccgatcgac tgttagctgc ttgtcccaac ttacgccaag gctatcagtt ataccaagac
     2341 attctttatg cggtaaagaa aagagatcag tcactatttg aggattgttt aactagagac
     2401 attagcggtc tgccagaaac ctatacaacc actttacgaa cgtttaaaaa gtttctgcca
     2461 cagatccaaa atgcgcttca ctacagctat tcaaacggac cattggaatg cttaaacaac
     2521 cacatcaaag tattaa
//
LOCUS       DAYYCQ010000169         2399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000169 DAYYCQ010000000
VERSION     DAYYCQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.177"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHAYF_002923"
     CDS             207..2069
                     /locus_tag="ACHAYF_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8580269.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2399
                     /locus_tag="ACHAYF_002924"
     CDS             2069..>2399
                     /locus_tag="ACHAYF_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8580270.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DAYYCQ010000170         2271 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000170 DAYYCQ010000000
VERSION     DAYYCQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.178"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..>2271)
                     /locus_tag="ACHAYF_002925"
     CDS             complement(151..>2271)
                     /locus_tag="ACHAYF_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8580271.1"
                     /translation="TSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIA
                     RLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQ
                     VTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNS
                     EQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAER
                     ASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQ
                     SYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEI
                     LKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSA
                     FDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKAS
                     ELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPN
                     ESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKR
                     VQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYT
                     TAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLD
                     EQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          580 a          507 c          413 g          771 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag t
//
LOCUS       DAYYCQ010000171         2178 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000171 DAYYCQ010000000
VERSION     DAYYCQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.179"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1038
                     /locus_tag="ACHAYF_002926"
     CDS             <1..1038
                     /locus_tag="ACHAYF_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFM8580272.1"
                     /translation="HLINILNVYQNFYRESKGDLFIYSEFENARRYSERIKELGLFND
                     VVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTKILFSNVYKKNP
                     DVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDV
                     KNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVN
                     SIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFS
                     PSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWA
                     ELRKLVEYGII"
     gene            1025..>2178
                     /locus_tag="ACHAYF_002927"
     CDS             1025..>2178
                     /locus_tag="ACHAYF_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8580273.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLG"
BASE COUNT          748 a          269 c          341 g          820 t
ORIGIN      
        1 catttgatta atatattaaa tgtttatcaa aatttttacc gagaatcaaa aggagattta
       61 tttatttatt ctgaatttga aaacgctaga agatatagtg agcgtataaa agaattaggg
      121 ctatttaatg atgttgtttt tgtgaatcac cggaaaatcg gttcttcctt taaacgtaaa
      181 attaacttgt taactaacca taatcaattt ttctcggatg atttatccgt ttatgatgaa
      241 atttttatac agggggaaaa ttatttcaca aaaatacttt tctccaatgt ttacaaaaaa
      301 aatccagatg taaaactaaa ttatatagaa gatggtttgg gtacttattt gaagaagagt
      361 atattttcaa caagtaaaaa cgttcaaaga gtttttaaaa tattaaataa aaatagcatc
      421 ttcaaaaaaa aaattgacga ttattttgtc tattgtccgg aattgttact ttatgatgta
      481 aaaaatataa aaaaaattcc caaaatagat gaaaacagta aattgatgaa aaacatacaa
      541 tcaatctttg ggatagaaga agtttttgtc ggagataaat gtatcttttt tgatcaacct
      601 ttattatctg atggttatgg catagatgaa acagatattt tcgctaaagt aaatagtata
      661 ttttctggaa aaaaagaaac tttaattaag cttcatccta gagcaaggac aaatcgttat
      721 ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact tgaaaatgac
      781 ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc atctatgatg
      841 tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga atatacagca
      901 ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga acattataac
      961 cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag aaagttggta
     1021 gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca gtttctggta
     1081 tactataggg aattttttta ttaagggaat ttcattcata actattccta tttttgtaaa
     1141 tattactact caagcagaat atggtttgat taataatttc acttcatttg tatcaatttt
     1201 ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt ttgactataa
     1261 agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt cgttaattgt
     1321 gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg atatgaacca
     1381 acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta gttatatcca
     1441 gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt ttatatctac
     1501 ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg atgataaata
     1561 tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag taatttattt
     1621 aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat acgctttaaa
     1681 aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac aattcgatag
     1741 aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct acattagtaa
     1801 cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg ttccatggtt
     1861 ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta actattattt
     1921 agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt tagcaaaatt
     1981 tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa ccttaggcta
     2041 tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga aaaaaacatc
     2101 gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa acatgatttt
     2161 catacctagg ttaggaat
//
LOCUS       DAYYCQ010000172         1991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000172 DAYYCQ010000000
VERSION     DAYYCQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.180"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..1061
                     /locus_tag="ACHAYF_002928"
     CDS             114..1061
                     /locus_tag="ACHAYF_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8580274.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            1191..1931
                     /locus_tag="ACHAYF_002929"
     CDS             1191..1931
                     /locus_tag="ACHAYF_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580275.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          695 a          328 c          427 g          541 t
ORIGIN      
        1 actcatgacg atagaggatt ttccgttttt tattttgggc tataataaac tctgttagga
       61 aaatttggta taattgatag taagaatgac aacatgttag aaaggaagag agcatgccca
      121 ctttagcaga tgttgcaaaa cgtgccaatg tttcaaaaat gacggtctct cgagtcatca
      181 atcatccgga acaggtgact gacgaattga aagagttagt tttctcagca atggctgaac
      241 tggattaccg tccaaatatt gctgcaaaag cacttgtcac gaatcggtcg cagatcatca
      301 aattatttat actggaagaa atcgacacaa ctgagcccta ttacatgaat ttgctgatgg
      361 gaatcgctaa acggatcggt aaagcgcatt attctctcca attagttaca aatgatgcct
      421 tcgacgtcgg ttcttgtgat ggatacatca tcacaggtgt cagaaaaaaa gacttcgatt
      481 ggatcgaccg tttggaaaaa ccagttgtct tgtttgggga aaattctgaa ggatatgatt
      541 atgtagacag cgataatgaa tatggtacag caaaagcaac gcaatatgcc ttagaaagag
      601 attatcaaac gattatttat atcggtatcg aagtggacga gccttttgaa atttcaaggg
      661 aaactggtta tttatctgtg atgaaggaag ttgatcttcc tagtaaaatc ttgcggtttc
      721 caaatcattc gacagaagta gaaagatata tgacagaaaa ctgggcagat tttacagata
      781 aaacttgttt tgtatgtagt tcagatcggc tagcactagg agcagtaaga gggattataa
      841 atgctggagg acacttgccc gaagaatttg gtgtgattgg atttgatggg gtatttttag
      901 accaaatcag ttcacctaaa ttgacaacga tcaagcaaga tattatacgt atgggtgaag
      961 tatgtggaga aatgttgtta aaaaaaatag aagaaaaagg ggcgagtcaa ggttatcgtc
     1021 gtttttttcc tgaactagtt atcagagaga caacgaatta agtcttatta ctatatatat
     1081 atttatgaaa ttcatcgcta ttttcttaaa ataatcagcg cattgctact gaaaacaatg
     1141 aatctgtgct attattgaga aaagttccat ttttgagaag gatggtttga atgaaagaaa
     1201 tcagacgaaa acatttccgt tttcctgcgt acgatgatga gttaggcgta aaattaacca
     1261 acgacaagaa gcgagtatta tttgacggac aagaagatct tttgactgaa cagaatcagt
     1321 ccccgtttga acagatgaaa gaatttcatt tcacgaacac gaacaccgag aaaatccgtc
     1381 gcaagcgtcg tgcaacaaat ggattgaagt cagctccgcg taaggaaaat ttggaaaagc
     1441 ataaaggaaa attaccggat tataccggcc attaccaatc agaaatcaca tcgacaggac
     1501 gaaaaaaact ttttggtgat tcctctcgtt ctgtcagaca aagtgaaaca aggacagagt
     1561 ccaagcatac tacacgaaac gcatctgttg acacgaaggt gcgggagcgt tcttattttg
     1621 ttcctaaata tgtacccgca tcgattatcc cagaccctag agaaagcaaa atttctgagg
     1681 atcatttaat cgaatcgatg aaaaaaacgg atgactctta tctgttgttc gatacagaac
     1741 cgacgccgta tcaagaaaaa agaaatggtg agccgtctgt acgaacattc aatcgaacaa
     1801 aaacgttaga ggaaacaaag cagtcagaag aaaaaaaatc gaaaaaaagc catggtatct
     1861 tagaacgctc attacaagga ttgatcgatg atcaaggaaa ctctttggga gaaaatagct
     1921 attttaaata agtgatatac aaatttatat tcggctacag ttggaaagga aacgctgcgg
     1981 cgtttcctta t
//
LOCUS       DAYYCQ010000173         1955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000173 DAYYCQ010000000
VERSION     DAYYCQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.181"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="ACHAYF_002930"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="ACHAYF_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8580276.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="ACHAYF_002931"
     CDS             853..1041
                     /locus_tag="ACHAYF_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8580277.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="ACHAYF_002932"
     CDS             1054..1611
                     /locus_tag="ACHAYF_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8580278.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="ACHAYF_002933"
     CDS             1643..1885
                     /locus_tag="ACHAYF_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8580279.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          716 a          304 c          404 g          531 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggtt
//
LOCUS       DAYYCQ010000174         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000174 DAYYCQ010000000
VERSION     DAYYCQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.182"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHAYF_002934"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHAYF_002934"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8580280.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYCQ010000175         1847 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000175 DAYYCQ010000000
VERSION     DAYYCQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.183"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="ACHAYF_002935"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="ACHAYF_002935"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8580281.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DAYYCQ010000176         1841 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000176 DAYYCQ010000000
VERSION     DAYYCQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.184"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..1605
                     /locus_tag="ACHAYF_002936"
     CDS             13..1605
                     /locus_tag="ACHAYF_002936"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8580282.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            1598..>1841
                     /locus_tag="ACHAYF_002937"
     CDS             1598..>1841
                     /locus_tag="ACHAYF_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8580283.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          639 a          300 c          389 g          513 t
ORIGIN      
        1 gagtgtatct ttttgggtgc agaattaaat caaaaattat ttagtgcggc agataatctc
       61 cgtagcaaaa tggatgcgtc agaatataaa aattatttat tgggactgat tttctataaa
      121 tatttatccg accgtttgtt ggaacaagtg gttcttttag cagatgaatc cttagaggaa
      181 tatgatacgg taagcaaaca aacgatgttg tatcgcgaat tactttcaga tgaagaatca
      241 aaagaagatc tgattgcaac aatcgttgat attttggggt atgcgatttc gccagagtat
      301 cttttcaacg tcttagcgga tcaagcaaaa caagcaacgt tccaattgaa tgatctaaac
      361 aaagcctttg tgcaattggc atctacctac aatcaattta atgggttatt tgatgatgtg
      421 gatctccaat caaaaaaact aggaacagat gagcaacaac gaaatgtgac gattaccgaa
      481 gtcattaaga aattgaatga cgtggaagtt ttaggtcacg atggggacgt gatcggggat
      541 gcttatgaat ttctgatcag tcaatttgct tcagaagcag ggaagaaagc cggagaattt
      601 tacacgcctc atatggtttc tgatatgatg gcacaaatcg tgacgttgga tcaaaaagaa
      661 cgacgattct ttagtgtttt tgatccaacc atgggttcag gttcattgat gttaaatgtg
      721 cgtaactact taactcaccc agataacgtt aaatatcatg ggcaagaatt aaatacaacg
      781 acatacaatc tagcaaaaat gaacttgatt ttacatgggg tagatgccga agaaatgaat
      841 ctccgcaatg gggatacgtt gaataaggat tggccaacag atgaacctta cacatttgat
      901 gcagtagtaa tgaacccacc ttattcggct aattggtcag cagatacgac cttcttagac
      961 gattctcggt ttaatcgtta tggaaaatta gcgccaaaat ccaaagcaga ttttgcgttt
     1021 cttcttcatg gtttctatca tttgaaggaa acagggacta tggccatcgt cttgccacac
     1081 ggagttttat tccgtggcgc tgcagaaggg gtcattcgtc aaaaattact agaagacggc
     1141 agtatttatg ctgtgatcgg tatgcctgcg aacttgttct tcgggacatc gattccaacg
     1201 acggtgattg tattgaaaaa gaaccgccaa actcgagacg tattattcat tgacgctagt
     1261 cgagaatttg tcaaaggaaa aaatcagaat aaattatcgg aagaaaacat tcaaaaaatc
     1321 ctcgaaacct atgctgaaag aaaagatgtc gagaaatacg ctcatttggc aaactttgat
     1381 gaaatcaaag aaaatgacta taacttaaat attccacgct atgtagatac gtttgaagaa
     1441 gaagaaccaa ttgatatggt tcatgtcgga aatgacatca agaagatacg acaagaacag
     1501 caagtgctag aaaaagaact tttagaagct atctcttccc tgcaaacaac acctgaaaat
     1561 gaagcgtggt tacaaggtgc gctggaggtg ttcaaacatg agtaatgata tacaaccaga
     1621 aattcgtttc ccaggattta cggaagcttg ggaacagcgt aagtttgaat gcttacttga
     1681 taaaaaagat ggagtaagga ggggaccatt tgggagtgcc ttaaagaaag aattctttgt
     1741 atctaatagc aattttgttg tctatgaaca acaaaatgct atttatgata attacgaaac
     1801 aagatataaa attactgaaa aaaaatataa tgaattaatc a
//
LOCUS       DAYYCQ010000177         1767 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000177 DAYYCQ010000000
VERSION     DAYYCQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.185"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..204)
                     /locus_tag="ACHAYF_002938"
     CDS             complement(<1..204)
                     /locus_tag="ACHAYF_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8580284.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTL"
     gene            complement(201..854)
                     /locus_tag="ACHAYF_002939"
     CDS             complement(201..854)
                     /locus_tag="ACHAYF_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8580285.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1142..>1767
                     /locus_tag="ACHAYF_002940"
     CDS             1142..>1767
                     /locus_tag="ACHAYF_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8580286.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          563 a          320 c          239 g          645 t
ORIGIN      
        1 caaagtttgt tctatatatt ttatttcttt tccattcata ttactagaaa tttcatttgc
       61 catattatga atcttttctt ttctcgtctc caaatatgtt ttaggaaaga taaaaaaaat
      121 taatgaatga actaatataa ttattattcc aagaacagaa aatatttgta tgaacatttt
      181 tggaaatatc tttaattttt tcattttctc tccaatttat atcctacatt tcttatagta
      241 gttatacagt ctaattgtaa tttttttcta agttctttta tatatacatc tattactcga
      301 tcataaggag tttcttcgct atctttccat acataatcaa taatttgcat tcttgttaac
      361 acttgtccat tattatccaa taaacatttt aatacttcta gttcttttgc atttacatct
      421 atttcttcac catttatttt agctgtatag ctattaaaat taaccgatag atccttatat
      481 tcaaacttct ctaaatgtcc ataattcttc ttaattaaag aatctattct agcttttaag
      541 accggcaatg aaaatggctt ttctacatat ccgtctacta aattagtaaa tgcatcaatt
      601 ttatattctt catcactaaa tgcagtcaag attagaattg gtaaattgct tttctttcta
      661 atttctttca acacttctaa accatttata aaaggtatct gaatgtctaa gataaccaaa
      721 tttatatcgc tattaaattt tgacaaggct tctcttccat ctttagcttg aataacagta
      781 tatccaaatt ctgaaagata ttcacttatt ccctctctta tcatattatc atcttcaaca
      841 gttaatattt tcatatatac ctccacttaa gcctatacac tagatattat acagcatatt
      901 atggctctat aatatctagt gttggtgaca ccttctgttg ccaatacttt ttcttttttc
      961 taataaatgc aaacagacac cttgaaatcc tttagaataa agggttctat aatattttgt
     1021 gttgttggaa aatcgaaggc gattttctac taacactttt tcttcgtttc aactcaaaaa
     1081 gagactcact tttggtaaaa tttagtcgac caaaacatca ccctaccaaa ggagtcgctc
     1141 tatggataat catactagaa aattactcgg attaacagat aaacatttat tttttgatga
     1201 agaatggctg attgaaaaag aatataaagg cgttcaagcg cagtttatta aagggagatt
     1261 ggatgactca ccttcccatt gtgaacactg tggatcgatt cgtattatcc gcaacggttc
     1321 ctatacgaca cgtactcaga tattgaaagt caaagaaaag ttgactattt tagaattaaa
     1381 acgtacccgt tttctatgct atgattgtgg gcagaccttt tctgcgaaaa cggacttagt
     1441 tgatgaacat catcaactaa caaaggaact gaagcaggcg attctcatag aactgtacga
     1501 aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc tcggatggga cggtgacacg
     1561 gatcttgcgt gaagcaacga agcattacca gccacgtatg aacttccttc cgacggtgtt
     1621 gtgtatggat gagttcaaat caatgaagtc tgtttctggt tccatgagtt ttatttgtgt
     1681 tgatgggaca acccatcagc tatttacgat ccttgaagat cgcagacttt acaaactaac
     1741 tcaatacttc ctgcgctttc ctagaaa
//
LOCUS       DAYYCQ010000178         1747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000178 DAYYCQ010000000
VERSION     DAYYCQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.186"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            378..1628
                     /locus_tag="ACHAYF_002941"
     CDS             378..1628
                     /locus_tag="ACHAYF_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8580287.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          334 c          308 g          527 t
ORIGIN      
        1 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
       61 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg gaaaggatcc
      121 aacgggaatt tgctatttcc tcctgtagcg tttgtctacc ctgtttagtc tgttacccga
      181 accactgtta tcactaccaa ctcattagct gtttcgataa tggactagac tttcaaatag
      241 tagtttggaa ggcagcttct agctctttta tcaccgtatt ctattaggcg aggttgtggc
      301 tatacagtga gtttctggga aatcccacag tatcgaagca ttttccaaaa tttatatcga
      361 aaggaggtcc ttcttccatg aaattatttg tcggtattga cgttagctct gaaaaactag
      421 atgtttgttt tttaacagac gacaatcaat tgtctatctt atctgaaatc tctgttgcca
      481 atgacatcga aggtgcttca ttcatacgag aaacaattct tgaattcaat aacagctacc
      541 actttgatca aattgtgatt ggcatggagt caacgtccat gtacagcttc catccttcca
      601 tgttttttca tgaagatgag aaactaaaga agctcaacac acttgtaacg attgaaaatc
      661 cttttcgggt gaaacaattt agtcgcatgt ttgatgagaa taaaactgat cgaaacgatg
      721 cattaagaat cgctgatttc ttgcgtattc aacgattcac aacttcccct atcaaagaag
      781 agaaatacat ggccttacag cgtttaacac gtactcgcta tcaacttatt aagcaattga
      841 ttcgtacaaa gcaacatttt cttgaaaatc taacgtacaa atgtaatacg cttgctcgag
      901 aaatgcgaga cgaatcgacg agtcttttta gtgcaaccct catttcactc atgacggaag
      961 atttcacatt ggatgaactt gctgagcttc ctttagaagc tttctgtgat ttacttcaag
     1021 aaaaaggaaa aggccgtttc aagcaaccag aaaaaatcgc taaagcaatt caacgtgcaa
     1081 ttaccatgag ttaccgccta ggtgctcttg cacaagaatc aattaatgtt gttttaagtg
     1141 ttctcgtacg agaaatacga gcacttgaaa aaaatatcaa agaattagat aaagccatcg
     1201 aacaagtaat tgttgtcatt ccagaatatc aatgcttaac cagtattcca ggcgttggta
     1261 aagtctatgc tgccggttta atcgctgaaa ttggtcaaat cgaaagattt gaagatcaga
     1321 caaaattggc aaaatacgct ggattaagtt ggaaagtgaa ccaatctgga aactatcagt
     1381 cgcaaaatac acctctgaca aaacaaggaa atcgatattt tagatactac ttagttgaag
     1441 ctgccaactc tgtaaaaaat tatcttcctg aatacaaagc tttttatcaa agtaaatata
     1501 aggaggttcc aaagcatcaa cacaaacgtg cactcgtcct aaccgcaaga aaatttacgc
     1561 gtctggtgga tacgctacta cgtaaacacc aactctatac gccaccaagg agtgtgatag
     1621 agaaataaca atccgttatt gacgcaaatc cttgaatcaa cccgaaaaaa atttgatttt
     1681 gatcgggtct agtttcgtgc gcttcaaaac atattactca ataaagaaaa tcttaaattt
     1741 catcttg
//
LOCUS       DAYYCQ010000179         1635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000179 DAYYCQ010000000
VERSION     DAYYCQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.187"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHAYF_002942"
     CDS             <1..1604
                     /locus_tag="ACHAYF_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8580288.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          598 a          269 c          333 g          435 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attca
//
LOCUS       DAYYCQ010000180         1626 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000180 DAYYCQ010000000
VERSION     DAYYCQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.188"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHAYF_002943"
     CDS             316..570
                     /locus_tag="ACHAYF_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8580289.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHAYF_002944"
     CDS             571..888
                     /locus_tag="ACHAYF_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8580290.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..>1626
                     /locus_tag="ACHAYF_002945"
     CDS             1447..>1626
                     /locus_tag="ACHAYF_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580291.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILS"
BASE COUNT          570 a          249 c          310 g          497 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttct
//
LOCUS       DAYYCQ010000181         1483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000181 DAYYCQ010000000
VERSION     DAYYCQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.189"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHAYF_002946"
     CDS             64..972
                     /locus_tag="ACHAYF_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8580292.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1483
                     /locus_tag="ACHAYF_002947"
     CDS             972..>1483
                     /locus_tag="ACHAYF_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580293.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          511 a          216 c          345 g          411 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DAYYCQ010000182         1480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000182 DAYYCQ010000000
VERSION     DAYYCQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.190"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHAYF_002948"
     CDS             64..972
                     /locus_tag="ACHAYF_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8580294.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACHAYF_002949"
     CDS             972..>1480
                     /locus_tag="ACHAYF_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580295.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAYYCQ010000183         1476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000183 DAYYCQ010000000
VERSION     DAYYCQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.191"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..501
                     /locus_tag="ACHAYF_002950"
     CDS             91..501
                     /locus_tag="ACHAYF_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8580296.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            515..871
                     /locus_tag="ACHAYF_002951"
     CDS             515..871
                     /locus_tag="ACHAYF_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8580297.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          541 a          210 c          272 g          453 t
ORIGIN      
        1 tttgattttt tttatatttt attagctaat aattggtatt ttaaaagaaa ttactgtaga
       61 attggattat aattaaaaag gagtgagaaa atgataaaag tatatacttc gcctagctgt
      121 acttcctgta gaaaagcaag agcatggttg caagcgaatc aacttgaatt tgaagagaag
      181 aatatttttg ccgaaccgtt aacagaaagt gagatcaaaa agatcttggc agcgactgaa
      241 ggaggagttc aagatattat ttccactcgg tcgaaaattt acgaaaagct gggcatagat
      301 tttaacgaat taactttaaa gcaaatgatc gtcttattca aagaatatcc ttcattattg
      361 agacgaccta ttttgattga tgaaaaaaga atactaatcg gatttaatga agatgagatc
      421 cggtcatttg tccctcgtga aattcgatgt gttgccagaa atctgatcta tttacaaaac
      481 aataacatct taaattcata gggagggagc cttcgtggaa gtgaaagtct atggatcagc
      541 gcactgtgta acaagtctga aggcgaaaca atggttagaa caccatcaat tgccttatca
      601 atttgttgac ttagaagaac gcgatttaag tacagaagaa gcagaagaat tatgcagttt
      661 tgaagaagtg caagcagaac agctatttgc gacttggtct gaggcttttc acaacattgc
      721 gattgacctt tcctgtgtag gaaaaaaaca actgattttt ttatgcacac aacataccaa
      781 tctcctcaga cgtcctttga ttattatcga tgaagttcta tttattggat acaatgagca
      841 gttgatggaa cagcttatct taaataaata gactttaact gttttaatca agtcactgag
      901 catttcatac agttgtaaca gtcgaacact tagcatcaag cgtgttatgt gttctttttt
      961 tgactttatt tttctactat agagacatgc tcaatttatg tggtcaaaat tcaaatctag
     1021 gtggggatat agagttaaaa atatattgac atgtgtaaat ttacatgata tattcatttc
     1081 tgttgtttta agtagcaaag caataaaata gtattttaaa aaacatataa aatagttgtt
     1141 gacatgttag aaatgacatg ataagataac tgagttgtta aaaaagaacc acttgtgaag
     1201 cttgattcta tcgaaaaaaa cttcaaaaaa gttcttgaca aataacaaat gataagttat
     1261 gataaatgag ttgctgtaag atttcgaaaa acgaaatcaa aaaaactttt aaaaaagagt
     1321 tgacaattac ttgtctctct gatatgatat aaaagttgct acggcagcca aaaacgaatc
     1381 ggggaacact cctgatgaag tagacctttg aaaactgaac aaagtaagac aaaccaaatg
     1441 tgtagggcgt cttgattcaa ttcaagacaa caaaca
//
LOCUS       DAYYCQ010000184         1475 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000184 DAYYCQ010000000
VERSION     DAYYCQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.192"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1184)
                     /locus_tag="ACHAYF_002952"
     CDS             complement(45..1184)
                     /locus_tag="ACHAYF_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8580298.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          387 a          314 c          266 g          508 t
ORIGIN      
        1 aataatgcta atctggctct gttttttcat ttattaaaaa aatcttacgc taagtaggat
       61 aaaataattc cttgatattt ttcgatcata tccaaataat tatcgatttc aacatactca
      121 tctacttgat gaggcaaagt aacgacacca ggcccaaaga cgacaaagtc gaaggaatgg
      181 ttagctttcg taaattcggc tgcatccgtt gttgctgccg ctccaactaa aggtaatggc
      241 tggctgaatt gttgctgaat gcaatgaatc aaaggagaat ctggatcagc tttgacaggt
      301 attttgttgt aatcgatcat caattccaaa tgatagtctg tctcttgatt caattcattg
      361 acgattgatt gcagcaatgc aatgatttta tcatttgggt attctggaat cgatcgaatg
      421 ttcccttgga gctgcgcatg gcttgggatg ctgttgactt gattcccgcc actgatcaat
      481 gtaacattat ggattgtttt tcctaggaca ggattctcga ttgtttcagc gaggtgattc
      541 atttcagcat ttgctttcgt aatgaattca tttaggtgat tgattgcatt ataaccttgg
      601 tctggcatag agctatgcgc ttcttttccg tgcgaggtga ctgtatagtt gattgatccc
      661 atgtgcgtat acatcaaact gtaattggtt ggctctccga tgatcaaggc atctaagtca
      721 tctacatagc ctgctttcgt gagctgttcc ccacctagtt cacccacttc ttctccgaca
      781 gtggctaata atttgactgt tccgttaaat ggttttcctg actctttcag ttcgatcatg
      841 gcaatcacca ttgctgccaa gccactcttc atatctgtag caccacgtcc gtacaaacgg
      901 ttcccatgga tttctgctgc aaagggtgca taggtccaag acgattcatc accagccgcc
      961 acgacatcca tatgcccaga taggcctaac acttttcctg attggccctt ttggtaacga
     1021 gcaatcaaat tatctctacc atctctgtaa gaaacaatct ctcctgttat gtcatgcttt
     1081 gccaaaagct tattcaaata ggctgctact tcaccttcgt ttccattcac tgatttaatt
     1141 cggatgatct cttgtaaaat cgcgattttt tcttcttttt tcatctaatc ctctcccttg
     1201 ccatttcttt ttattgtatt tgagtgtgct cgaattttca aataggagca accaaagtaa
     1261 aaatgaagaa agagagccac tttatgaaaa atatgctgct acttccctcc tattttattt
     1321 atacgagagg acttgctcat agattcctat tctactgttc tatccttaaa agctcttata
     1381 tgatgctagt tattgttagc tttccttttt caaggatgat cttttcttgt accattcttt
     1441 gtaaacatca tatacagaag ctagttgcaa aaaaa
//
LOCUS       DAYYCQ010000185         1432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000185 DAYYCQ010000000
VERSION     DAYYCQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.193"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..243
                     /locus_tag="ACHAYF_002953"
     CDS             <1..243
                     /locus_tag="ACHAYF_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8580299.1"
                     /translation="SRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLI
                     LDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            248..1195
                     /locus_tag="ACHAYF_002954"
     CDS             248..1195
                     /locus_tag="ACHAYF_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8580300.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          471 a          201 c          298 g          462 t
ORIGIN      
        1 tctaggcaac agacacctga agtttttgaa atgaatgcct caatgtattc ttttaaacct
       61 gaatttctaa aaaaaagtaa ttatattttt gatgggcaat gtgggataat aaaaatgaga
      121 gattatctaa ttttagatat tgatagtgaa gaagatttta aatggttaga atttttgttt
      181 cctaaatttt tagaagattc ttcggagctt caagatatct ataattattt atatcggagg
      241 taaaaggatg agctgttatc ttatcaccgg tggggctggc ttcattggtt ctaccttagc
      301 caatcattta ggagaagaac atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt
      361 agaaaatctt gatatgacga aaaatatcac ttttattgag ggagatgtcg ctgagaaatc
      421 cttgatggaa gagatcatga aagctaataa atttgattat atctttcatt tagcagcagt
      481 tgctagtgtg gcagattcag ttgcccgacc agtagaaaca catcgtgtga attttgaaag
      541 tgtattaatg attttagaat tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt
      601 tagttcttca gcggctgttt atggggatga accgacttta ccaaaaaaag aagagtccgt
      661 gattcgccca ttgacgcctt atgcggttga taaattcgct gcagaacaat atgtgctaga
      721 ttattgtcat ttatatgatg tacccggtag cgcagttcgt tttttcaatg tctatggtcc
      781 aaatcaaaat ccgaattctc catacagtgg tgtgatttct attctcgtag atcgctataa
      841 gaaacaaatc gtaggagaag caacttcatt tacattattt ggtgatggaa gccagtctag
      901 agactttgtt tatatagatg atgtaatcca agcattactt ttagttgcga atgaggaagc
      961 tgcactgggg aaacaattca atgttggaac gggtaaagca accacacttt tggaattgat
     1021 tcatgcaatc aatgaaattc tcggtgtaga attgacactt gaatacaaag aagagcgttc
     1081 gggtgatatc catgactctc tagcagatat tactaagatc cagtcaattg gctatcaacc
     1141 gaaatatggt attttaagtg gaatgactaa ttatttgaaa actgaaatca attaaaacat
     1201 actaagataa gattagaggg taaattcagg ctctttgtca ataaggactg atgagttttg
     1261 taaaatcaaa tctgggatag aagggctctt tgtcaaatga tgttggtaaa gagaaatctt
     1321 agaataattt ggacagaaca gaaagaagaa aacctctttc tgttctgttt atttggttaa
     1381 aaatgttttg ccatgacgaa tcataattcg tagtttgaaa ttgtaaaagt tg
//
LOCUS       DAYYCQ010000186         1374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000186 DAYYCQ010000000
VERSION     DAYYCQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.194"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1360
                     /locus_tag="ACHAYF_002955"
     rRNA            <1..1360
                     /locus_tag="ACHAYF_002955"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          373 a          297 c          418 g          286 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg acttaaccaa taggttaaaa gaaa
//
LOCUS       DAYYCQ010000187         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000187 DAYYCQ010000000
VERSION     DAYYCQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.195"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACHAYF_002956"
     CDS             82..>1364
                     /locus_tag="ACHAYF_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8580301.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAYYCQ010000188         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000188 DAYYCQ010000000
VERSION     DAYYCQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.196"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHAYF_002957"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHAYF_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM8580302.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAYYCQ010000189         1266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000189 DAYYCQ010000000
VERSION     DAYYCQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.198"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..392)
                     /locus_tag="ACHAYF_002958"
     CDS             complement(243..392)
                     /locus_tag="ACHAYF_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580303.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="ACHAYF_002959"
     CDS             854..1066
                     /locus_tag="ACHAYF_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580304.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          471 a          188 c          209 g          398 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaa
//
LOCUS       DAYYCQ010000190         1246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000190 DAYYCQ010000000
VERSION     DAYYCQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.199"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHAYF_002960"
     CDS             200..742
                     /locus_tag="ACHAYF_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580305.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACHAYF_002961"
     CDS             898..>1246
                     /locus_tag="ACHAYF_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8580306.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAYYCQ010000191         1235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000191 DAYYCQ010000000
VERSION     DAYYCQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.200"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..788
                     /locus_tag="ACHAYF_002962"
     CDS             <1..788
                     /locus_tag="ACHAYF_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8580307.1"
                     /translation="KIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYE
                     NKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGM
                     DIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQF
                     IGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHT
                     ISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            805..>1235
                     /locus_tag="ACHAYF_002963"
     CDS             805..>1235
                     /locus_tag="ACHAYF_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580308.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSES"
BASE COUNT          471 a          135 c          215 g          414 t
ORIGIN      
        1 gaaaaataga agatctattc catgaaccgt taattgaata tacagaaaat ctaagtaaaa
       61 ctatcgagca aatcaaaata gataatctgt tatcacaaaa ttacgatcca gaaatatatc
      121 tgtttcttta tgaaaataaa atactggaat atgttaaaaa tggagatatt cgaaatttag
      181 aaaacatggt ttttaatctg agtaatggaa taattccaag tgttagtgga gatacgattc
      241 ggtctgaaaa aaattattct atcattgttt ttgaaaagtt agcacaaacg tctataacat
      301 taggaatgga tataatagaa gcgtatcaaa gtagagatgc tttgatacaa gaaaacgaat
      361 tagctgtatc gttacctgag gtcttaaaag tgagggattc tgggattgtt tattatacta
      421 aggaaatcgg aaaaactaag atagagcatc tttctccctt aatttcttct gtcgttcaat
      481 tcatcggctt aaatatctat aaaagaatta ctgtaaagga aatagctaat tatttttctg
      541 taagtgaaac taaattaaga gaaagcttta agaatgaaat gcatattacg atttataatt
      601 atatctctaa gcgaaaaatt tctacggcta aaataatgtt gaaatcaaac catactatca
      661 gtgaagtttc tttaggttta ggattttcag attcttcaca cttttcgaga gtattcaaaa
      721 agtacgcagg tgtatctcct aagcaatatc aattaggttt agtggacaat ttcaacaaca
      781 ttagctgaga ggttttattt tattatgaga ctatatcatt tacgtagttt ggaggagttg
      841 aggaaattgg attttaacaa aaaatttttt tgggaaattt ctgatttgag ttgtttcgta
      901 gaatctgagc gtctaagtaa tggtaataat agtgtgttaa atgaatatga caaagtagta
      961 attaataaag taaaaagttt atgtgaagaa gctagagaat ctatcagtca tattaatggt
     1021 agcaatttta aacaaacatt atttaatctg attaagctag atgctaaaat atcaagttat
     1081 ttattctttt tgatacatga tgaatttatg gattatcaaa aattggatca gttaattgaa
     1141 agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt aaacaactac
     1201 aagttaatag attataagta tttgagtgaa agcaa
//
LOCUS       DAYYCQ010000192         1219 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000192 DAYYCQ010000000
VERSION     DAYYCQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.201"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..460
                     /locus_tag="ACHAYF_002964"
     CDS             2..460
                     /locus_tag="ACHAYF_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8580309.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="ACHAYF_002965"
     CDS             469..696
                     /locus_tag="ACHAYF_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8580310.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          377 a          195 c          204 g          443 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     1081 gctcccatat atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac
     1141 tatgtagttt tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa
     1201 cattattttg ataataatg
//
LOCUS       DAYYCQ010000193         1214 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000193 DAYYCQ010000000
VERSION     DAYYCQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.202"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..129)
                     /locus_tag="ACHAYF_002966"
     CDS             complement(<1..129)
                     /locus_tag="ACHAYF_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8580311.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTD"
     gene            342..>1214
                     /locus_tag="ACHAYF_002967"
     CDS             342..>1214
                     /locus_tag="ACHAYF_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8580312.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNYGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSAASKRNN"
BASE COUNT          408 a          204 c          218 g          384 t
ORIGIN      
        1 atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata tgttagaaac
       61 agttctcata atttccttga atgttataaa agtatctgta taatgttcag tatattttga
      121 tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg cgtttttttg
      181 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgggt
      241 ttttattacg ccgattgtaa aattaagcta gacaactaaa aaagtcattt gtgctacact
      301 caaaatagtt ccgaccaaag aattataagg agtgagtaca aatgacctat acccatctta
      361 caacggatga acttgtaata atagagtctt atttcaaaat gaatcaatct gttgctaaaa
      421 ctgcccattg cttgaatcgt tcaagacaaa cgatccataa agtatatctg ttcttcaagc
      481 aaggaaaatc agccttagaa tattatcaac agtataagaa aaacaaatca aactatggta
      541 gacgtccgct tgttttaccc gaggaacaat cagaatatat tcaaagaaag gttgttcaag
      601 gatggacacc cgatgtgatt gttggtcgtg cagcgtttcc tattagttgt tctgctcgta
      661 ccatttatcg tatgttcaaa aaggggctat ttgattcttc tgacttaccg atgaaaggca
      721 agcgtaaacc gaatggacat caagaaagac gtggaaaaca aactttccgc cgctctattc
      781 atgaacgtga aaaggattat agccaattct caaatgagtt tggtcacctt gaaggtgaca
      841 ctatcgtagg tctgaaacat aaaagtgctg taattacctt agttgaacga ttatcaaaag
      901 ttatcatcac attgaaaccg tgtggtagac aagcgattga tattgaaaaa aaattaaatc
      961 aatggtttga atctgtaccg aaaaacctat tcaaatccat cacttttgat tgtggaaagg
     1021 aattttcaaa ttggaaacag atcagtaatg tcaatgatat tgccatttat ttcgctgatc
     1081 caggaacgcc gtctcaaaga ggcctaaacg agaattctaa cggattgtta cgtagagatg
     1141 gtttattgaa atctatggat ttcaattcag tagatgaatt ttttattcaa tctgccgcat
     1201 ctaaacgaaa taat
//
LOCUS       DAYYCQ010000194         1212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000194 DAYYCQ010000000
VERSION     DAYYCQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.203"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..>1212
                     /locus_tag="ACHAYF_002968"
     CDS             110..>1212
                     /locus_tag="ACHAYF_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580313.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYT"
BASE COUNT          403 a          157 c          156 g          496 t
ORIGIN      
        1 tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat atagtgacta
       61 atctagtgtt taaaaaaaat aaatgatact atatgtaata taaaaaaaca tgaggtggta
      121 tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat ttgttagttt
      181 attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg atacggcatt
      241 tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta attttgaaac
      301 gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc tatatccttt
      361 atttttattg atcaagttta ctaatttagc aataggctat aacctttttc tttatattgt
      421 tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca aaaaacatgt
      481 tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg tagaaatatt
      541 tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta ttttactcgg
      601 gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga ttagcatgac
      661 tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga ttataattgc
      721 tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta ttaaattaat
      781 aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta tgattgagca
      841 gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa gagcaattta
      901 tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat ttggtattgg
      961 cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat taggaatctt
     1021 atctaaaatc acttatataa tgggtataat atctattcta ctttgtacaa aaatatttcc
     1081 atggtttgcc ttacaaaata ctcctttaaa tattattcaa gctccttggc gttttatgat
     1141 attttcaaca ttgttcatat ctttttcagt atctctattt atttctccac agttggaaaa
     1201 attttcttat ac
//
LOCUS       DAYYCQ010000195         1173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000195 DAYYCQ010000000
VERSION     DAYYCQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.204"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHAYF_002969"
     CDS             <1..969
                     /locus_tag="ACHAYF_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8580314.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          396 a          209 c          234 g          334 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cag
//
LOCUS       DAYYCQ010000196         1084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000196 DAYYCQ010000000
VERSION     DAYYCQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.205"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACHAYF_002970"
     CDS             288..695
                     /locus_tag="ACHAYF_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580315.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..>1084
                     /locus_tag="ACHAYF_002971"
     CDS             709..>1084
                     /locus_tag="ACHAYF_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580316.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGK"
BASE COUNT          405 a          135 c          186 g          358 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaat
//
LOCUS       DAYYCQ010000197         1062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000197 DAYYCQ010000000
VERSION     DAYYCQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.206"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACHAYF_002972"
     CDS             complement(37..>1062)
                     /locus_tag="ACHAYF_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8580317.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAYYCQ010000198         1045 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000198 DAYYCQ010000000
VERSION     DAYYCQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.207"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHAYF_002973"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHAYF_002973"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8580318.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DAYYCQ010000199         1035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000199 DAYYCQ010000000
VERSION     DAYYCQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.208"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="ACHAYF_002974"
     rRNA            complement(<1..>1035)
                     /locus_tag="ACHAYF_002974"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAYYCQ010000200         1002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000200 DAYYCQ010000000
VERSION     DAYYCQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.209"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1002
                     /locus_tag="ACHAYF_002975"
                     /pseudo
     CDS             369..1002
                     /locus_tag="ACHAYF_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAYYCQ010000201          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000201 DAYYCQ010000000
VERSION     DAYYCQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.210"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHAYF_002976"
     CDS             <1..>986
                     /locus_tag="ACHAYF_002976"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8580319.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAYYCQ010000202          959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000202 DAYYCQ010000000
VERSION     DAYYCQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.211"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACHAYF_002977"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACHAYF_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACHAYF_002978"
     CDS             complement(570..>959)
                     /locus_tag="ACHAYF_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8580320.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAYYCQ010000203          945 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000203 DAYYCQ010000000
VERSION     DAYYCQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.213"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..541)
                     /locus_tag="ACHAYF_002979"
     CDS             complement(20..541)
                     /locus_tag="ACHAYF_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580321.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLM
                     ELKKYSNTQTKFS"
BASE COUNT          295 a          163 c          134 g          353 t
ORIGIN      
        1 tattgtattc tttataactt caagagaact tcgtttgagt attactgtat ttttttaatt
       61 ccataagaaa attagttatt acctgccacc taatcagcaa atctgaacct gagtcaatat
      121 aattaatatt attttccatc tccaacattg taagtactgt tggtgttaat atagggactt
      181 tgtacaattg tatttttttg attttatcat ctgcacaaag gagatttttt aaatctaatc
      241 cttcttttat ttcaaaagtt gaatttttta aatctataag agtaaaacca attccttcat
      301 actgaatttc tgaaaatcga atttttctcc cagttgatag atcaaggggc agaaaacctt
      361 ttattacatt gtgagtgtca ccatcattta caatgtcatt aggaattttg attttagaat
      421 tctgtggagc acctattgga agataataat tcattataat tttgtagtct tcatgaggaa
      481 ataaagtact tttaggattg ctaagatatt ctcgtaccga atctaagaat ggcttatcca
      541 ttgaatcttc atccccattt aaactaaaaa acattcctac aattgctttt gcaactctaa
      601 gtgaattcat tttctttatt gaatgtgtta aatcagactt accattcttt aaacttgtat
      661 tgttataaag gaaattttta ttgtcttctg caaatctaat atattcctca acatactcac
      721 taccaaaaaa actattacat ttactacata acgtattgga atggcaacag tttttttgac
      781 aaattttata aggtgcagaa cttctttccg tatgctattc cgattgtcct tgacaatgag
      841 cctcctcgga tgtgacgatc ttcggcagga gctaacagtt aaagttagac tgcctcgcta
      901 gcgttagcac atccgagctc attatcaagg attcgctgcg ccaat
//
LOCUS       DAYYCQ010000204          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000204 DAYYCQ010000000
VERSION     DAYYCQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.214"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHAYF_002980"
     rRNA            <1..>943
                     /locus_tag="ACHAYF_002980"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYCQ010000205          872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000205 DAYYCQ010000000
VERSION     DAYYCQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.216"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>872
                     /locus_tag="ACHAYF_002981"
     CDS             <1..>872
                     /locus_tag="ACHAYF_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8580322.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYR"
BASE COUNT          379 a          113 c          142 g          238 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aa
//
LOCUS       DAYYCQ010000206          858 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000206 DAYYCQ010000000
VERSION     DAYYCQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.217"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..116
                     /locus_tag="ACHAYF_002982"
     CDS             <1..116
                     /locus_tag="ACHAYF_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFM8580323.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
     gene            244..795
                     /gene="raiA"
                     /locus_tag="ACHAYF_002983"
     CDS             244..795
                     /gene="raiA"
                     /locus_tag="ACHAYF_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8580324.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT          306 a          151 c          177 g          224 t
ORIGIN      
        1 aaaaaatatt gattattgac gatgtctata caacaggaca gacgcttttt catgcagcag
       61 attgcttgca gaacgcacag ccgaaaatca tccgaaccat ttctttggca agatgaatca
      121 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
      181 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
      241 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
      301 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
      361 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
      421 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
      481 ttggttgttg acaaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
      541 tcccgtgaaa cgggcatgaa tactgcagat gctgctgctt tattcaatga agaggacgaa
      601 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
      661 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
      721 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
      781 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
      841 agctgccgca agtttttt
//
LOCUS       DAYYCQ010000207          849 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000207 DAYYCQ010000000
VERSION     DAYYCQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.219"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..720
                     /locus_tag="ACHAYF_002984"
     CDS             <1..720
                     /locus_tag="ACHAYF_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8580325.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          326 a          133 c          167 g          223 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatata
//
LOCUS       DAYYCQ010000208          824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000208 DAYYCQ010000000
VERSION     DAYYCQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.220"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(357..470)
                     /locus_tag="ACHAYF_002985"
                     /pseudo
     CDS             complement(357..470)
                     /locus_tag="ACHAYF_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          295 a          129 c          131 g          269 t
ORIGIN      
        1 attgaaacat ccatctggaa tttgtctttt tcgttgcttt gctcataaaa atctgcatat
       61 tcatcactaa tattttttct aataaattct tccatcataa agatcacctc gaaaagagta
      121 tacgaacaaa cgttcttttt gtaaaggtgg aatttattat actaaataaa taggtgaaca
      181 agtacttgtg ccaaattatg tgccaaaaaa aatcgaattt aataaaacac aaacaaaaag
      241 aaatcctatt atgataacat ttcttataat aacaaacaca acagaagacg tgtaatagtt
      301 agtcaggaac gtacaaataa cccctgtatc ctttgcggta cagggggtta ttttgtataa
      361 gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt
      421 actccttgat aaagtaaaat agatgcatgc gtatgtctta gtccatcaat aaacagtcga
      481 agagaaaata gggaaaaatt gattactaaa gaaaaactta ctaggtcgac ggtgtagttg
      541 attttcatac ttcaatagat tcgaattaaa cgatttcgat tggcttatac cagtcctatc
      601 caacgaaaga tatatcgttc ttaactctag cggtataact tgaagcttta cgtaaagcca
      661 atgggaatga actggagatg actttttatt atttttagta atagctgatg ggttaatact
      721 tacaactttc cttttcaaag taccatataa aaaaacggtg gggttgttta gcgaaataaa
      781 ggatctaagc caatagctta gatcctttat tctatgcttt atat
//
LOCUS       DAYYCQ010000209          716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000209 DAYYCQ010000000
VERSION     DAYYCQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.221"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..>716)
                     /locus_tag="ACHAYF_002986"
     CDS             complement(47..>716)
                     /locus_tag="ACHAYF_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580326.1"
                     /translation="NLNDLSRSISEYLKGNSYLKIRFSHILILLPYLNENFTLIQVDK
                     SFIASGNQGNMNITSNKTSRVLLALMGYGILYRDDQNGQILINRAGRSLLMKYRKFIP
                     EDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNELLNKLYPENAIKAHFGDANHMY
                     SWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYVEDEDIIKYMKPVIKLVNKNKGT
                     RGAS"
BASE COUNT          222 a          133 c           85 g          276 t
ORIGIN      
        1 tttgttgatt taccaacatg tttagacatt tttctctagg aaaaaattaa ctagcaccac
       61 gggttccttt atttttatta acaagtttta tcacaggctt catatatttg attatatctt
      121 catcttctac atagatatct gttatcatta cgacatctgt actatttcga ttgagtactt
      181 tcacttgatc tccagcattg attttccctc taaatctttt aggaatgaac caactataca
      241 tatgattagc atcaccaaaa tgtgctttta tcgcattctc aggatataac ttattcaata
      301 gttcattctt ttgaattttc aaaacttcaa atctatactt atcatcttct gctaatctaa
      361 ataaatcttc taaacgcatg ttttctaact gtaagttttg aaaatcttcc ggtataaatt
      421 ttctatattt cattaataat gaacgcccag cccgatttat tagaatctgg ccattttggt
      481 catctctata aagaattccg taacccatca acgccaatag cacacgtgac gttttgttac
      541 ttgtaatatt catatttcct tgattacctg aggcaatgaa agacttatct acttgaatta
      601 gggtgaaatt ctcattcaaa tatggtagta aaattaatat atgtgaaaat cgaatcttga
      661 gatagctatt ccccttgaga tattcactga tacttctact caaatcattt aaattt
//
LOCUS       DAYYCQ010000210          691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000210 DAYYCQ010000000
VERSION     DAYYCQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.222"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..282
                     /locus_tag="ACHAYF_002987"
     CDS             <1..282
                     /locus_tag="ACHAYF_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8580327.1"
                     /translation="QLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNKNY
                     FKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(318..689)
                     /locus_tag="ACHAYF_002988"
     CDS             complement(318..689)
                     /locus_tag="ACHAYF_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580328.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          223 a          112 c           96 g          260 t
ORIGIN      
        1 caattatggt ttaaatttga cccttcgaat agatttatca aagattttta taaagtatgg
       61 aattcagaag ttttcttttt agcaatcgaa gatagcttat taatcaatct ctactattct
      121 aataagaact atttcaaaat tcctgctgcg aaaactagaa tgaagaaggg tgtatacttt
      181 ttgtttgata tcgtgactga cgtgccggac gcacgtagcg atcatcggcg ttatgactat
      241 ataaagtata ctttcgttga tccagaaaga tacaaagatt aaataaaaaa ataacaaaca
      301 aactcgcttg aaattcatta ggtgagtttg tttattattt tttctgcttc tcgattcatc
      361 agcgccatat ctgttttcgt aatggtttca ttaggaatat agtcagcaca ttttcttttc
      421 tctaacattc tatacccact attatgtatt cctaaggtat tcaattcctt aactgttgaa
      481 aacaaattcc aaacggtttg gtggcttcct tttccgctac ttccattata aataatatca
      541 taattttctt tcaaatactc atcagatttt ttaaaagcac tgtagtatgc tcttgaaaca
      601 gcagttcttc tataagattc tttagaatta tatttatcat ctgattcaat ttgtttggaa
      661 atagataaat attcattaaa gtcaaacatt t
//
LOCUS       DAYYCQ010000211          625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000211 DAYYCQ010000000
VERSION     DAYYCQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.223"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..625
                     /locus_tag="ACHAYF_002989"
                     /pseudo
     CDS             1..625
                     /locus_tag="ACHAYF_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DAYYCQ010000212          576 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000212 DAYYCQ010000000
VERSION     DAYYCQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.224"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          186 a           81 c           81 g          228 t
ORIGIN      
        1 ttttttcttg atgtgatatc cttgtctttt ttgtaatgtt tttaacttat tgcttttgca
       61 aaatttattt acctgttttc tggatcaacg atagtgtaca ttatgctatc cttgtttgga
      121 ttttaggacg tttaaagagg cactttttta cataacttaa tccatagacc ttttaagcat
      181 gctgtgagat aataataaag aagagtttaa agcgcacccc aaaccacttc cccataagtg
      241 tattacgctt taaactcttt tatatttgaa ttcactaaga agcataccat atttttgaaa
      301 aaaagtgaga aaaaaggctt ataattagga tttagagtaa ttaattagat gtttatttta
      361 taggactgat actatagatt acaaatagta tcgtatttcg caatcttaaa attatcttct
      421 tactgtgaat aagataataa aatttgtatt tgtccttttt aggtaactta ttttaatcta
      481 tatacctttc tggatcaacg aaagtatact ttatatagtc ataacgccga tggtaactat
      541 gcactgtaac gataatgctg ctaaaatatc ttttta
//
LOCUS       DAYYCQ010000213          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000213 DAYYCQ010000000
VERSION     DAYYCQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.225"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHAYF_002990"
     CDS             <1..>534
                     /locus_tag="ACHAYF_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8580329.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAYYCQ010000214          525 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000214 DAYYCQ010000000
VERSION     DAYYCQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.226"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>525
                     /locus_tag="ACHAYF_002991"
     CDS             <1..>525
                     /locus_tag="ACHAYF_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8580330.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDH
                     RPVKRRNKFYRSLRT"
BASE COUNT          183 a           90 c          103 g          149 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt
      421 ttatcaaggg acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca
      481 tcgtccagta aagagacgca ataaattcta tcgaagttta cgcac
//
LOCUS       DAYYCQ010000215          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000215 DAYYCQ010000000
VERSION     DAYYCQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.227"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACHAYF_002992"
     CDS             <1..>468
                     /locus_tag="ACHAYF_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8580331.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAYYCQ010000216          464 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000216 DAYYCQ010000000
VERSION     DAYYCQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.228"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>464
                     /locus_tag="ACHAYF_002993"
     CDS             <1..>464
                     /locus_tag="ACHAYF_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8580332.1"
                     /translation="LVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSG
                     NSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVT
                     MSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          168 a           85 c           77 g          134 t
ORIGIN      
        1 ctagtcacta aaggcgattt aaatgaatta ttccgttcgc acttagaaaa agcgataaac
       61 acactcctac ggactgaatt aacggctttt ttagattacg aaaaatatga tcgcactggt
      121 tttaattcag gtaattcgag aaacggttct tactttcgat caatcaaaac cgaatatggt
      181 gaattaacat tggaaatacc tagagatcgt aatggtgagt ttaaacaaca aactttacca
      241 gcctacaaaa gaacaaacga tacattggaa accactatta tccatttatt cgaaaaaggt
      301 gttacgatgt ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca
      361 caaaccatgt ccaacatgac taaagttctg actgaagaag taaatgcctt taaatccaga
      421 gccttaaatg ataagtatgt cgctattttt atggacgcta ctta
//
LOCUS       DAYYCQ010000217          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000217 DAYYCQ010000000
VERSION     DAYYCQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.229"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHAYF_002994"
     CDS             complement(<1..430)
                     /locus_tag="ACHAYF_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8580333.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAYYCQ010000218          461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000218 DAYYCQ010000000
VERSION     DAYYCQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.230"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..359
                     /locus_tag="ACHAYF_002995"
     CDS             <1..359
                     /locus_tag="ACHAYF_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580334.1"
                     /translation="QNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTPEEQIQR
                     KLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIVKAKVSK
                     RFIFNPKLEIYSVVIE"
BASE COUNT          203 a           46 c           73 g          139 t
ORIGIN      
        1 ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt atagttttgg
       61 gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat actcctgaag
      121 aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat ccaagagata
      181 attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt gaagttacag
      241 ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta aaggcaaaag
      301 tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta atagaataat
      361 agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa ataaaatata
      421 ccatggaaga cataaaagaa aaactggata actagtttat t
//
LOCUS       DAYYCQ010000219          454 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000219 DAYYCQ010000000
VERSION     DAYYCQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.231"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..>454
                     /locus_tag="ACHAYF_002996"
     CDS             290..>454
                     /locus_tag="ACHAYF_002996"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8580335.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILK"
BASE COUNT          166 a           50 c           70 g          168 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaa
//
LOCUS       DAYYCQ010000220          437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000220 DAYYCQ010000000
VERSION     DAYYCQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.232"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>437
                     /locus_tag="ACHAYF_002997"
     CDS             <1..>437
                     /locus_tag="ACHAYF_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8580336.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGK
                     KWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV"
BASE COUNT          136 a           82 c          105 g          114 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 acctttacac aagaacagcc attggaagcc atcgttacgg atcttactta tgttcgcgtg
      301 gggaaaaagt ggcattatat ctgcttaata cttgatttgt ttaatcgaga aattattggt
      361 tattcctgtg gtgagaagaa agatgcctca ttggtaaaag aagcctttgg acggataccg
      421 tattctttaa cagacgt
//
LOCUS       DAYYCQ010000221          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000221 DAYYCQ010000000
VERSION     DAYYCQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.233"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACHAYF_002998"
     CDS             complement(109..>414)
                     /locus_tag="ACHAYF_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8580337.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAYYCQ010000222          379 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000222 DAYYCQ010000000
VERSION     DAYYCQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.234"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          137 a           61 c           62 g          119 t
ORIGIN      
        1 aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttacaatt ggagtggtag
       61 ttaattagtg acttattttt gattttatag cactgatact ataaaatata gatatcatca
      121 tattacacaa tcttaatact aacttaaaaa atatctcctt tcacaagtat ggtgataaaa
      181 ttcgttccgg gctacctttt taggtagcct actttaatct ttataccttt ctggatcaac
      241 gaaagtatac tttatatagt cataacgccg atgatcgcta cgtgcgtccg gcacgtcagt
      301 catgaatcgg ctacactaga ctagacagaa aaaataaggt gtgtagaata ataaaaaaca
      361 cactggagga tttttcatg
//
LOCUS       DAYYCQ010000223          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000223 DAYYCQ010000000
VERSION     DAYYCQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.235"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHAYF_002999"
     CDS             complement(<1..>321)
                     /locus_tag="ACHAYF_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8580338.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYCQ010000224          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000224 DAYYCQ010000000
VERSION     DAYYCQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.236"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHAYF_003000"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHAYF_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYCQ010000225          299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000225 DAYYCQ010000000
VERSION     DAYYCQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.237"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACHAYF_003001"
     CDS             <1..>299
                     /locus_tag="ACHAYF_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8580339.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAYYCQ010000226          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000226 DAYYCQ010000000
VERSION     DAYYCQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.denovo.238"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHAYF_003002"
     CDS             <1..>282
                     /locus_tag="ACHAYF_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8580340.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYCQ010000227        13054 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.guided.078, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000227 DAYYCQ010000000
VERSION     DAYYCQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.guided.078"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1034)
                     /locus_tag="ACHAYF_003003"
     CDS             complement(<1..1034)
                     /locus_tag="ACHAYF_003003"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8580341.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINE"
     gene            complement(1239..2432)
                     /locus_tag="ACHAYF_003004"
     CDS             complement(1239..2432)
                     /locus_tag="ACHAYF_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8580342.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(2511..2717)
                     /locus_tag="ACHAYF_003005"
     CDS             complement(2511..2717)
                     /locus_tag="ACHAYF_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8580343.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(2710..3852)
                     /locus_tag="ACHAYF_003006"
     CDS             complement(2710..3852)
                     /locus_tag="ACHAYF_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8580344.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            4364..4999
                     /locus_tag="ACHAYF_003007"
     CDS             4364..4999
                     /locus_tag="ACHAYF_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8580345.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            5135..5227
                     /locus_tag="ACHAYF_003008"
                     /pseudo
     CDS             5135..5227
                     /locus_tag="ACHAYF_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            5341..5976
                     /locus_tag="ACHAYF_003009"
     CDS             5341..5976
                     /locus_tag="ACHAYF_003009"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8580346.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            6083..7321
                     /gene="mef(H)"
                     /locus_tag="ACHAYF_003010"
                     /gene_synonym="mefH"
     CDS             6083..7321
                     /gene="mef(H)"
                     /locus_tag="ACHAYF_003010"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8580347.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            7548..7967
                     /locus_tag="ACHAYF_003011"
     CDS             7548..7967
                     /locus_tag="ACHAYF_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8580348.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            7972..8211
                     /locus_tag="ACHAYF_003012"
     CDS             7972..8211
                     /locus_tag="ACHAYF_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8580349.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(8436..9359)
                     /locus_tag="ACHAYF_003013"
     CDS             complement(8436..9359)
                     /locus_tag="ACHAYF_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8580350.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(9391..10155)
                     /gene="map"
                     /locus_tag="ACHAYF_003014"
     CDS             complement(9391..10155)
                     /gene="map"
                     /locus_tag="ACHAYF_003014"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8580351.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            10313..10753
                     /locus_tag="ACHAYF_003015"
     CDS             10313..10753
                     /locus_tag="ACHAYF_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8580352.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(10931..11500)
                     /locus_tag="ACHAYF_003016"
     CDS             complement(10931..11500)
                     /locus_tag="ACHAYF_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8580353.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            11755..12987
                     /locus_tag="ACHAYF_003017"
     CDS             11755..12987
                     /locus_tag="ACHAYF_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8580354.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         4053 a         2399 c         2439 g         4163 t
ORIGIN      
        1 ttctcattga tcagaccaat caattcaaat aataaaattg cagcaaacaa catggcaata
       61 atcaatatga tcgttccgat tcttccagta taattcatga ccatagctgt gaaagaaaac
      121 atcaaagcca tcgcataaat cgtcaggaca gcacctttat gagtaaagcc taaactgatt
      181 agtcggtgat gaagatgttt tttatctgct gtagaaaaag aaacacggtt tgcttttctc
      241 ctaatgatag caaaaaacgt gtctgttacc ggcactccca tgacgatcag caaagaaatc
      301 gatgaaacaa aagtcacgtt cttcagccct tgtagtgaaa ggacggccat cataaaccct
      361 aggaatagag ctcctgtatc gcctaaataa atctttgccg gataaaaatt gtaaggaaaa
      421 aaaccgataa tacttgctac aagacagaaa ataacaactg gaatatacac tgtagaagca
      481 tggaggaaaa aatatccaat gattcctatc gtaattaaac cgatcaatga aatccctgcc
      541 gccagcccat ccaaaccgtc aatcagattg accgcattcg tgataccgaa gatccaaaaa
      601 atcgttacgg gaaaactaag ccagcctagt tggatatatc caataaaagg aagcgtgaag
      661 gaatcaatac gtatatctgc tacaaaataa ataataagag aagcaatcaa gatccctata
      721 cttttttgtt taggtgtcag ctcatattta tcatcgagca ctcctgtgac tatgattact
      781 aaagaagcaa tcaagatcgg tacaatatac gattgaggga taattttacg gaataagaat
      841 aaagtcgata taacaaatga aataaagata ggcagtccgc ccgctgaagg cattggaact
      901 ttatttattc ttctttcatt tggtttgtcg accatacctg tttgtactga aatgattttg
      961 aatacaggtg ttaatatcag tgataataaa attgtcagaa aaaatcttat aaccatacta
     1021 aaagaaacaa gcatatgggc acctacttta attaagagtc ataattatta taaattatat
     1081 atgtcttttt gactatcttt ttatatcttc tcaaatgtcc gcaaagcctt attttatcgg
     1141 ctctacgggc attttgcttt tgtggtaaac ctcgcatatc taggtctatc ttcttatatt
     1201 ttccctgtca atcgtggtta aaatcgtggt aaatacttct atgcgattag acgctgaacc
     1261 tctgattttg cggtatctat ggacgcatga gcgtaccagt tcattgtgat actgatgttt
     1321 gaatgcccca tgatatactg taaatctttc gggttcatgt tcttgcttgc cagccttgtg
     1381 cagaatgtat ggcgtagcgt atgcggtgta atatgtggca agggattgtc cttgtggtgc
     1441 ttgttgtatt tctttaccat acggataaat aaggcgttat aatcaatggc aacttttgga
     1501 ttacctttcg gattgataaa cacaaaaccg ctgtgtccgt ctacctctat cgactttatc
     1561 tttggtcggc tgttgataac ccgttgaaat gcctgtatcg tttctttgct taatggcact
     1621 tgccttatcc cgcttttcgt cttaggcgtt tcaatataat agccctgttc cttactcttt
     1681 agtaactggt ggtcgataat cacaacttca ttcttgaaat caatgtcggc tactgtcagt
     1741 ccgcacagtt ccgagatacg aagtcctgtc tttaacagta tcagcacatc atcataatgc
     1801 ttgtgataca cgttgtccgt cttaataaat gacaataagg cttgctcctg ttcctctgtc
     1861 aatgcgactt tctctttggt atcattttct atgacttcac ttagcttgaa atcaaaaggg
     1921 tttttcctca cataatcatc ttgtatggct atgtagaatg acgcttttaa cgagcgttta
     1981 tggttgttaa tggtattgta ggaaaagcct ttgtctttca tgcgtaacgc ccattcttta
     2041 gcgtcagagg gttttatcgt atcaatgctc ctagcaccta acttgtcctc ttttaataac
     2101 cgcatgagtt gttcccgttg tttctgtgtg cttttcttca catttgtcct ctgtgcgttc
     2161 tgtttggcat agagttggca aagcgtcatt ttcttgcctg tgctgtcgat accgtcctca
     2221 atatcccgtc ttatctgctg ttccagttct cgaagtgagg gtttttcccg ttttcccttt
     2281 ggtgtcgggt ctgtgggtgt caatctccaa gcatacacat attttgtgtt tcccaatgcg
     2341 tctacatatt tatataagta ttttccgtct gttcgttggc tctctccagt atgcaggata
     2401 cgacctttgt tatcccgtct tttttctttc atggtgtctg ctcctttcct tgttggaaag
     2461 agccttgata tgacttgtgt tcatcataac acatacaagg ctcatttgca ttagattgcg
     2521 tccaatttat caataacctt ttcaaactgt cggcgtttaa tctgtatgcg gttgccattc
     2581 ataatgagcc agccagcgtc cttgttttcc tctgccaatc gtcttaactt gttttctcca
     2641 atgcggaaat actttgacgc ttcttcaatg gtaagggtgt atttttccca tacgggaata
     2701 tcctgtgtat tactcataaa acaccccctc actttcgatt acgtctgata tgtccgtaca
     2761 catctgttcc acaagcattt cctgtttttc agaaaggtgg gtacattcat caatcatttt
     2821 catgtagttt gaacctgtca gcctgtcgat ttcctctatc atcacgattg acggcatgac
     2881 ctgtctttcc agccattgca gagtgcgttc catattgaca ggtacagggt tcatttcaaa
     2941 aggtaaactg tcatgggaaa tgaatatctc ccacaatgga tagtcaggaa attccacaaa
     3001 atcggtaatg agataaaaca ccagcccatg agggttatac gtcaataaca gttcttcaac
     3061 tgcctgtacc gcctttttgt cacgcagacg aatttcaaaa cggttgataa tatcggtatg
     3121 tttctttttc gttgcctgtt ccttttgttt ttcatacagg cagaaatatt ttgtgcttgc
     3181 ctttgagccg atataaaggg tacttgctaa attacggttt tttccgctta attttccacc
     3241 ttgtacattt tcatagtttt tacagcgaca ttccgcacca ccatttctgt atttttcaga
     3301 tagcacagga atatccagca gaccgcacat atcattgatt gccaagtcaa aacgtcggat
     3361 aacaccgcca cagtcaagac agcggtttaa gaatgaatac cagtccctgt tctgtgcctg
     3421 taagacatat tccatgttgc gtgagcctgc cccttttaat tctaaaaata cgcccatgtg
     3481 ttcatcatca gaagccatga ccttgatttc cccgtaagca taatgctcct tatagccata
     3541 atacccatag tcataatgga taaaataatc tgctttcatg ccaagcacct ttcggataac
     3601 ttccaatgcg tcagtcgttg gaaaacggac agacagataa tcaacgagca gataaaaagg
     3661 cttatcgcag atatagtaat tcagacaacg caggataatg ttctgtgttt ccttgctggc
     3721 gttagctttt ccgttctcaa agcggttgat actatatctt gatacatggg taagacttgc
     3781 aagttctcct tgtgaaagac cgctggcaag acgtttgttc ttcatttcaa gtgcaaattc
     3841 taaattgttc attgtattcc tccaatccca caatagcctt gaaatacctg taaaccacct
     3901 ttcaaaaagt gtgatatgta ctatcttctt aaaacctctc attactactc acaggtttgg
     3961 gggtgtatgg tttttgactt gtcttttgtg aaatagtgcc cccctgttag ataacggggg
     4021 cacagacaag gctcgcaggc tcgccagccg acaaagcatt tcttcccctt gacacaaggt
     4081 gtcatggcgg taagcctgag aggcttccgc caccgtgtca gaaatacttt gtcagcaagt
     4141 tctgttttgc cttttctaac aagttccatt tcaaccgata ttcatatcta cggatttaat
     4201 cagataggta tttctttttg aatgttttta tatttcattc tattgtgctt attcgattac
     4261 aagaacatag taattcttat atgcttattt gtcaatatct ttttttgcct ttacaaatca
     4321 tattaagcat gatagactta taatagcgaa gatataacga gatatggagg gttatgcaat
     4381 gaaaataagc catttaggaa ataatataca gaccataaga aaatttaggg gaatgaaaca
     4441 acaggaactt gcggacaaaa tcggtatcaa tatgcagagc ctttccaaga ttgaaagagg
     4501 gttgaactat cctgcctatg aaacgctgga aaagataatg gaagtgctgg acgttacgcc
     4561 caatgaattg ttgtcgggag aatggaagta tgtcaatcaa tctgaaaagg aagtctgtca
     4621 gtttttaaga gccgaagaac gtctaaatgc agaactgaaa catggacact atgataactt
     4681 ctttgacagc gaggaagaat ggctggaata tgagttagaa aagttacggg aatatattac
     4741 agactatatc aacgggaaaa gcattgaagc gtccgacctt tacccaatca aagaatttat
     4801 ccagcatttg aagtttcaaa aattattaga ccgctatgat gatttataca gtatggatat
     4861 gtttggggaa agcatagagg gacataagta taagacacct tatcaagtcg tgaaaatgat
     4921 aaacccgaac tcaaaagaag atatggaact gttacgagaa gtattaagga ataacaattt
     4981 tgatgatgag gacgagtaac ttttttcctc ttgcgagaag atacagcaaa caaaaagccc
     5041 gatcaagagt gcaaagcacc accaatggtg gcaaagctct tgacagggct ttttcttttc
     5101 tgttgtgggc aatcaagagg aagaaaggga taaaatctga tttgtggtaa atcaggcaaa
     5161 ggcaacgcag gtatcacagt atgaattgac accgcataag gaaagcaact ggaagattgt
     5221 agaataaatg aaaaaggctt gcattctgca tttttataaa attgcaagtc tttatattga
     5281 caagcataaa atactaagat atactttaat cagaccaacg gtttgtattg gaggtaagtt
     5341 atggcacgaa ataaatatcc agaagaaaca gtaaaactca ttttagatgt ttctacaaaa
     5401 atgttttgtg aaaaaggata tgataacaca tcattgcaag atattatcaa tgaaacaaaa
     5461 ctttctaaag gtgctattta tcatcatttc gattcaaagg aagatatttt aaaagcaatt
     5521 tttcaaagaa ttggaaatga aaatgcagta atatttgcta aaattagaga taacgatagt
     5581 ttaaacggtt tagaaaagtt aagagaaata tttaagacag cggtattcaa ttctaaccaa
     5641 agcgttttat tgacagtttc tcctcaacta ttaaacaatc ctcgttttct tgctatgcaa
     5701 atagaacaaa tgtatgaaat tgtagtacct gaatttgttg aacccgtttt agaacaaggt
     5761 atcaaggata agtctttgga gattgaaaat tcacatgaaa ttgcagaagt tatcatggta
     5821 ttaagtaatg tatggctcaa cccattagtt gaaatgacgg ataaagaggg tatggaaaaa
     5881 cgttgcaata cattcaatgc ccttttaaat ggaataggca tagacacatt attagacgaa
     5941 gaaacaattt ctgcatttat acagttttgt aaataactgt atttgttatc cctatacgat
     6001 agactgctct ataaaagagc agtttattta aaccattata taaaccgacg gtctgtttga
     6061 ttaaataaaa aggagagatt ttatgcaagc agaaaaaaaa ctttttaatc gtgattttat
     6121 acttgttgtt ataggacaga taatttcttt atttggaaac gctatcctac gatttgcatt
     6181 accgtactat ttgttaaaag aaactggttc atcagctttg tttggtattg ctacagcctg
     6241 ctctttttta cctatggtta ttttgtcgct ggtaggtggt attattgctg acaggacaaa
     6301 taaaagaaac attatggtca tactagattt tgtaacagca atcataattt tagtatttta
     6361 tttgtcattg ggaaaaattc ctatcatacc tttgtttatt gttgctttga tgttacttta
     6421 tggtatttct ggtgcatatc aaccagcggt acaagcaagt attcctctat tggtatcatc
     6481 agataaactt accgtcggta atgctataat aaatcaagta aatactttag caaactttat
     6541 cggaccagct ataagcggag tagtttttca attttatggt atcaatccca ttttaattgt
     6601 tagtattata tgttttactt tttcagccat tatggaaatt tttatccata ttccacacca
     6661 aaaaagacct cgtgaagtag gtgtaattgc agttgctaaa aacgatttaa aagatagtta
     6721 tacctttttg aaaaaggaga aacccatttt tatatccgta atctttttgg tttgcatttt
     6781 caatttggtt ttaagtgctg ttttaattgt tggtctgcct atcttaattt ctgaagtttt
     6841 aaaaatgcca gatacactat atggtttttc acaatcagca ttatcattag gtggtttatg
     6901 tggtggtatt ttaacagctc ttgttgcttc aaagttaaaa ctaaaaaacc tttatacctt
     6961 gttacttatt tgttctgttt ctgttttact tatgggaatt tcgctatttt tagaattgcc
     7021 tgcaatagtt tcctactggg tagttacagt gatgtgcttt gtggctatgg gtgtatctac
     7081 tatgtttgtt gttcaaattt acactcttgt tcagacacaa acaccaccgc aattagtagg
     7141 aaagataatg gctacgctgg tagcagttgc tatgtgcggt caacctattg gacaaatggt
     7201 ttacggtatt ttattcgatg tatttataca agaaacatgg gttgttatgt ttgtagcttc
     7261 tattgcttca atcatgattt caatatattc aaaaagaatt tttcaaaagt tagagatata
     7321 atacaagggg ttaaatagta tgttaggcta aaaacaatat ataaaagaaa atttgaaatc
     7381 tgctaaaagg atagtgttat aaaagaaaga gtaatattgc agtatttaag gctcgcacaa
     7441 atatatgttt tgtgtgggct tttttcttac catttttctg tatttctaaa gggtataaac
     7501 ggtattccgt ttatatattt cagcattagc agaaaggggg tgagattatg aggacatctt
     7561 ctttcgagca tatcgtcaga ttacagttca attctttaat gctgattgtc attaagaaca
     7621 aagtgaaaag tcgaaacaga cagcttgcaa gacattctaa acgtgaagta ttgttttgtg
     7681 aaatagccga aacgaaacaa gttgaacgtg gaacgattga tacctgtttt tatgattgtg
     7741 tatcatttaa agtcttgcac tttacaattt atgtatctga tgaaacactt ggtacagttt
     7801 tacatagact gtcagaaaaa cagcgtagtg cgattttatt acgttatttt caaggaatga
     7861 acgacagaga aatttcagaa ctgtaccatg tatcacgttc tgctatttgc agtagaagaa
     7921 ctagaggact aaggaagtta aaaatgcttt tgagcgaaag gaagtaaaga aatgagtaaa
     7981 gattatggct atccctcttt tgaattgatt tgcaaagcgt caagcggaga tgaaatggca
     8041 gttaaagaga ttttgaagtt ttatgacggt tatatttcta aattatgctt gcgtcctttt
     8101 taccactctg aaagtggtaa aatcattatg caggttgatg aagaactaaa aggagaaatt
     8161 catacagaca tgatgaaagc aatattgaaa tttgaaatcg gagtgaagta atcaactgta
     8221 taaaaaaatc gggagataca cagtttcgtg ctatctccct tagtagtttt acggtcatat
     8281 caaggctcat tcaatcgtgg taaattcgtg gtaagatgag tgtttttcta tacttcaaaa
     8341 tgcttgaatg tatagtgttt atgcggataa ttgaaaatta gatataaaat ttcaaataaa
     8401 ttactgatat aaatttacaa taagaactct tgtttctact tttttttatg agaaaattta
     8461 gttttcaatt tattgaacca ttttcggctt ggatctcgac gttttttgat ttgatgaccc
     8521 gtcacgtatt cttgaacaac tgcattgatg attccgccca aaatgatgat cgtagcagca
     8581 aaattaagcc aaagcatgag tacaataaaa cttcctatga tctgataacc gctgatcctc
     8641 gttgcaaaat acctagcata gatactaaac acttgtgata acagcatcca gccgacagta
     8701 gcgaagatcg tgcccggaat gatcgtcttc aacttcaact gagcattagg gacaattaaa
     8761 taaatcaaga acatgatgac aaataaagct accaatgtga ttggccactt caaagtttgg
     8821 aactgatcga taaagttcac gggtatttgt acgattggct gaatagcatc caaaatcagc
     8881 tgtcccaacc caagaacgat agttacccca ctaatagcaa tcatgaacaa gacaatgaca
     8941 attagtgaga agaaacggac gatcaaaaaa ttttttctat tttctacgcc atacgcttta
     9001 ttcatcgcaa tttgcaaagc attgatactt tgacttgcag accataaagc cgctaatgca
     9061 gaaacggata acaagcctcc tgaactttgt gtcaaaaggt ctttgattgc aggaccgata
     9121 aactggtaaa cggtctcagg aatcacttct cgtaaatagg tcagtacagt ctcttgatcg
     9181 atgtggagaa acggtaaaat attaccaaaa gcaataatca atggaaataa agacaagagt
     9241 aaataataag caacgacgac agaggtcgta ctcatttctg aatcagtgat gcgcttttga
     9301 gtcgtctcaa taaagcgcat cagattttca ttttgtttta ttttgttcca aactttcatg
     9361 tacctctccc gtacctattg actggataga tcaataagtg ccttcttctc cttgtgatgt
     9421 caaaattctt ggaccatctt tagtaatagc caatgtatgt tcatattggc aagaaagtcc
     9481 gccgtctctt gtatacgcag tccagccgtt aggatccatt ttcattttcc aagttccggt
     9541 attaaccata ggttcgatcg tgataaccat gccttctttc aaccgcaacc cttttccagc
     9601 ttctccgtaa tgaggaactg ctggttcttc atgaatcgta ggaccaacgc catgaccaat
     9661 aaaatcacga acgatcgcta gattttcgcc ttctacataa gtttggatag catgaccgat
     9721 gtcaccaatc cgattcccta cttgtgcttg ctcaataccg atgtacaaag ctttcttcgt
     9781 tacttccatc aatcggtcga tctcaggtgt agattctcca actacatagg cccaacaaga
     9841 atcagaaatg ccgcctttca aatcgataca catatcgact tttaccaaat caccgttttt
     9901 caaaggcttt ttacgaggga atccatgaca aatttcatca tttatactga tacaagtcgc
     9961 atattcatac ccttcaaagc caatttgcgc agcaattccg ccatgactct cgatatagtt
    10021 tctgacaaat acctcaatat cccaacttgt gattcctggt tttataaaat cacgcagatt
    10081 tttatgtaca tctgctaata aagctccaga ttctgccatc atttcaattt cacgtgctga
    10141 ttttaaagta atcatatttt cgtctccttt ttacaaatcc ctctcatttt aacatagcgg
    10201 catacattta gccaaatgac attccccagg tgtctctagg ttttattttc ttttcaatga
    10261 tgttttggta taattcataa ttgttaaata attataaacg aggaggcaca tcatggcttt
    10321 agcaaaaatc gtatatgcta gcatgacagg aaacactgaa gaaatcgccg atattgttgc
    10381 agaagcattt gaaaatctgg atatcgaagt cgaaatcaac gaatgtaccc aagtagatgc
    10441 agatgaattc gaggaggcag atatttgcgt agtagctact tatacatacg atgatggcga
    10501 tctgcctgat gagatcgtag acttttatga agacttgcaa gaattggatc tattgggaaa
    10561 aatttacggt gtctgcggat ctggtgatac tttttacgat gaattctgca aatcggtgga
    10621 tgatttcgat gctgcttttg caaaaacagg cgcatcaaaa ggagccgaaa atgttaaagt
    10681 ggatctgaat gctgaagaag aagatatcga aaatttagaa gctttcgcta aagaactagt
    10741 cgcaaaaatt taatatactt gcaataaaag agccatccag atttattcaa tctgtatggc
    10801 tcttttattc attgcattca tggaagagtt cttcgggagt taaaaatgca tacgctgtaa
    10861 gaaaaacgaa cttttcctta ttttgacaca tctcttgtta cctactgatt tgtggaactt
    10921 ttgagtatat tcaatcgatt tcgattggtt ctttgaattt caagatagca tctatactgt
    10981 cttggatcat cttttcaatc tgttcatcag ttggatggat gatgcttgaa gacatcaaag
    11041 ctgcgatccc agtagctatg atccatgtcc cattatacaa agattcgatc tgctcttctg
    11101 aaagattcac ataagctgga tcgcgtttca ctgtattcac aaagtaagta taagagaaat
    11161 tctgcatctc ttttcctcca ccatattctt ctaagtacag tgaacgatac aacttcttct
    11221 ctcttcttgc aaaatggata taattaaaag caagatcgat aatcgtattc cctgtatgct
    11281 caacagtgaa cacttcatat tgtaaatgat catagatctg ctcaaaaaga gcttgtctca
    11341 agtcatccat atttttaaac tcaagataaa tcggttgcgt ggaacaattc attttgttcg
    11401 cgatgtttct cgcagtaaat cgtgtaaatc catcggtggc agctacttca taagcagctg
    11461 ccaaaatctg ttctctagta atcgtctttt ttcttgccat attttttaat ctccttaatt
    11521 tttttataac gcttccacca ttgattaatt tcattgtaac atgttttttt tgatatgcaa
    11581 aattagtttt tgattttctt cacaagctga aatagcaagt agtaacatat atcatgaaac
    11641 ttaaccttaa aaaaataacc tccttttttt atttaaagcc tcattgtttg aatcataatt
    11701 aaatggtatc atgctaatag ttaccagaaa aaatggtgag gaggaatttt tattatgtta
    11761 tctgatttta atgaacttct aaaaaaatat gcacgcttaa ttgcagaaac tggcgtagcc
    11821 acagaaaaag gacacacagt tgttttacag atcagcgtgg aacaggcacc ccttgctcga
    11881 ttgatcacac aagaagctta taaactagga gccgctgagg ttatcgttca atggactgac
    11941 gaccagatcc aaagagaatt tcttttacat gctgcaacgg atcgtattga gaatgtacca
    12001 caatctaaaa tcgatcaagc ggatgaatgg cttgaaaaag gagcaagcag gatcagcgtt
    12061 gtttctgccg atcccgatgc atttgctggt gtagatagcc atcgagtagc gtcttatcaa
    12121 tcagcgaatg gcaaagcact aatgaattta cgcaaggcaa ctcaagcaaa taaagtcagt
    12181 tggactgtcg tggctgcggc tggtaaacaa tgggcagcaa aagtcttccc agaactgcca
    12241 gaagaaaaac aagttgaggc attatgggat cagattttca aaacgactcg tgtctatgaa
    12301 gaagaccctg tcttagcatg gaagaagcat gatgaaaaat tagcaaaaaa agcagaagaa
    12361 ttgaatcgag aacaattttc tgcccttcac tatactgctc ccggtacaga tatcattatc
    12421 ggattgccta aaaaccatct ttgggaaggc gctggaagct ataatgctcg tggagaaaaa
    12481 ttcatggcaa atatgccgac ggaagaagtg ttcacagcac ctgacagtca tcgagtagat
    12541 ggttacattt caagtacaaa gccactgagt tatgctggca ccatcatctc gggtatgaaa
    12601 tttactttta aagatggaaa agttgttgat ttttctgccg aacaaggaga agatgtctta
    12661 gcaaaactgc ttgatacaga tgaaggtgcg cgtcgtttag gagaagtcgc acttgtacct
    12721 gacccttcac ctatttctca atctggcatc atctttttca atacattatt tgatgaaaac
    12781 gcatcgaatc acttagcact aggttctgct tatgccttca gtgtgaaagg cggtacagaa
    12841 atgtccgatg aagaattagc agaagctgga ttgaatagaa gtcaaaccca cgtagatttc
    12901 atggtaggat ctgacaagat ggatatcgac ggaatccgtg aagatggctc tacagtaccg
    12961 attttccgaa atggcgattg ggcttgagta tactttcgca cacatgcaaa aaataatggc
    13021 tctttgtcaa taaggactga tgagttgtgc aaaa
//
LOCUS       DAYYCQ010000228        10486 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.guided.092, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000228 DAYYCQ010000000
VERSION     DAYYCQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.guided.092"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..707
                     /locus_tag="ACHAYF_003018"
     CDS             <1..707
                     /locus_tag="ACHAYF_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8580355.1"
                     /translation="RVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEIYHFDFL
                     EAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNY
                     FILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDN
                     QAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDS
                     TIYEQYEYDLSSVENK"
     gene            complement(857..1798)
                     /locus_tag="ACHAYF_003019"
     CDS             complement(857..1798)
                     /locus_tag="ACHAYF_003019"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8580356.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1924..3045
                     /locus_tag="ACHAYF_003020"
     CDS             1924..3045
                     /locus_tag="ACHAYF_003020"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8580357.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3303..4805
                     /gene="eat(A)"
                     /locus_tag="ACHAYF_003021"
     CDS             3303..4805
                     /gene="eat(A)"
                     /locus_tag="ACHAYF_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8580358.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4872..5399
                     /locus_tag="ACHAYF_003022"
     CDS             4872..5399
                     /locus_tag="ACHAYF_003022"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8580359.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5610..6671
                     /locus_tag="ACHAYF_003023"
     CDS             5610..6671
                     /locus_tag="ACHAYF_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8580360.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6674..7570
                     /gene="murQ"
                     /locus_tag="ACHAYF_003024"
     CDS             6674..7570
                     /gene="murQ"
                     /locus_tag="ACHAYF_003024"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8580361.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7584..9566
                     /locus_tag="ACHAYF_003025"
     CDS             7584..9566
                     /locus_tag="ACHAYF_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8580362.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9631..10077)
                     /gene="nrdI"
                     /locus_tag="ACHAYF_003026"
     CDS             complement(9631..10077)
                     /gene="nrdI"
                     /locus_tag="ACHAYF_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8580363.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10288..>10486
                     /locus_tag="ACHAYF_003027"
     CDS             10288..>10486
                     /locus_tag="ACHAYF_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine:ornithine antiporter"
                     /protein_id="HFM8580364.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINK"
BASE COUNT         3409 a         1850 c         2283 g         2944 t
ORIGIN      
        1 gacgcgtgcg cccatttacc aatgcaaaaa ttgtgttatt tttttggaat cacttcaaag
       61 cagaacacga ggcgatgctg aagcaggcac aagatgaacc ggcaatcgat gagatttatc
      121 attttgattt tttggaagca aaagaacttg ggctgaaaca taatagttca ttttattcga
      181 aacatatgaa tttttcttct agcgaaccaa agacggatct attttttggt gcaacgaata
      241 atgggagaaa agaacgagca gaaagctata agcaggaatt tataaaaaga gatgttacga
      301 ccaactattt cattcttcca catcgaggga atgaacaacc tggatatctt acttatgaag
      361 agtacctgaa aaaaacaggg gaatcgcgcg ggattctgga actgttgcgg gagggtcagc
      421 aaggagtgac tttgcggaca tttgaatcca tgtattttca aaagaaacta gtgacggata
      481 atcaagcgat tgctcattat catttttatc gtcctgaaaa tatttttttg cttcgagaac
      541 gtgatttaga tgaattaccg gaatttctgc atacaccatt taagccaatc ctatcagagc
      601 tattggaatt ttatgatgca gaaaattggg caaaacgatt tatcactaac gattccacta
      661 tttatgagca atacgaatat gacctctcct ctgttgaaaa caaataaaaa acctacattt
      721 tgaagagatt atgtactgaa acaaacaagc acaattctta tggcatcatg agagttgtgt
      781 tttctttttg atcttttatt gggcttaaaa aaagagaacc agctgctgat tcagctgatt
      841 ctcatcagag agccttttat tctgcgccaa ggattccttc ttttgcatgg actaattggg
      901 tcaaaaaggc acaatatggt gtagcaatat catatttttg tccttttcgc caaatggcac
      961 cattgatgta gtcgatttca gtcaaacgat ggtttttgat caggtcttgg tacatagatg
     1021 ggtaatgcaa cccgatgttg tcaggatcat aagtagaagc aatatgttgg tacacttctt
     1081 cttgatctag tatgattcct tcttttgctg cgatagaagc aaattcagat acgattgttc
     1141 gaaccatgct ctctgccgct ttttgcgcgc ctagttcagc aatattacaa tccaaaatcg
     1201 tgcataaacc attcaatgtt ccatttacac aagcttttcg ccaaatggaa taacgaacgt
     1261 tatggctata gatcggattt aaatttgctt cttgaaagac ttctacaact ttttttgtaa
     1321 atgcttcgcc ttctggttcc aagttctcta gttcgatttc tccatcacct aacaatgtga
     1381 cttttccggg accagctaga ccagctgtcc acatcgtgat cccaaataag atatttttct
     1441 ttggcacata tttttccagt acatcttcat gacctaatcc atttagtaaa cacaaaacat
     1501 aagtattgtc agtaataatg gattggatcg aacaaaacat atcatctaat tgattggctt
     1561 ttgttaatgc tacgatcaag tccacgtgtt cgttgctttc gatgatttct tctggtgaat
     1621 agattggcag ttttgcgacc acttctttac cgttaaagtc agcaatcaac ccatttgtac
     1681 gaatggcttc aatgtgagca ggccaacgat cgatcaagag gacctcattt ccgctttgat
     1741 gtagcatcaa tccgattcgg ctgcccattg ctccggcccc agctattgcg attttcatgt
     1801 tttttctctc ctttattcaa tgagttccct tttagtatat agaaaaatgg aagaaaatag
     1861 cattgttttg tttgaaaaaa atgaaaaaaa gtgtcttact taagaagaaa gtgagggata
     1921 cagatggata ttttggcggc aattgattca tttaaaggaa gtgcttcttc atttgaacta
     1981 aatcaggcag ttctaaatgg ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt
     2041 gcagatggcg gagaaaatac tgcagaagca atctattacg cgctcagggg aacatggcgt
     2101 accattgatg ggatggatct cttattccgt cagcgaaaaa tcagctattt attgacaact
     2161 tggcaaggaa aaaacaccgc agtcatcgaa tctgcacaag cattggggtt agatctactt
     2221 gataaaccaa cggatcaaac tatccgactt gcttccagtt atgccttagg agaacttatt
     2281 aaagatgcac tgcttcaaga agtagaacaa attattgtca ctttaggagg aagcgggtgt
     2341 agcgatggtg gtctaggatt actccaatcc ttgggtgctt ctttagaagg gtacacagaa
     2401 ggaaatcctt tattatctac taaaaaactt cattttgatc gaagaaacgt tcttgtttct
     2461 aaagtaaaat tgattatcgc tgcagatgtt accaatccat atacaggtca acaaggagct
     2521 gcttggcaat ttggaagaca aaaaggagga atagatacga cgctagcttt cttagataaa
     2581 caagcaggaa agattgctca gcaaataaaa gatttctggg aggtggattt agatgatatt
     2641 ccaggaagtg gagcagcagg cggtattggt ggagcccttt ttttgttggg agcgaagatg
     2701 gtttctggtt tcgatttagt cagccaaatc actggattgg aaaaaaagat cgaacaagca
     2761 gatgtcatca ttacaggaga agggcggatc gatcagcaaa cgatccacgg aaaagttcca
     2821 tacggtgtag cagtgcttgc aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt
     2881 agagaccaag agctagaagg actttctgat tttctcccaa ttgtcttaag tattcatttg
     2941 ggtccaatag gtttagaaga agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc
     3001 ttaggacaga cacttagcag atttttcacc atcctcccga agtagttgac aaaatcattt
     3061 tctttatgct atcgtaattt caaatatagg acaaagtgag ggatccgtgc attgaaaaat
     3121 taagtcgatt gatgaaagct gcattcagac tgtaccttgt ggagacaagg aagcagtgtg
     3181 cagattttcg ggattggctt ggttttttcg atgtatgggt ctttttgtgt gtatccaacc
     3241 cttgcatcct ctttagccag ttctgaaaat aggactggtc tttttttacg aggtgatgga
     3301 aaatgtctaa aatcgaaata aaaaatctga cattcggcta cgacagccaa ggcacattat
     3361 tatttgaaca agcaaatcta aattttgaca cacaatggaa actaggactt atcggacgaa
     3421 acggtcgagg aaagacaact ttactgaata ttctacaaaa caaactacct tatcaagggc
     3481 aagtaatcca tcagcaagaa tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt
     3541 taacctatta cgtgttaaat gatattacgg attttgagat atgggaaatc gaaagagagc
     3601 tccaattgat gcaaacagat cctgaaatct tatggagaga attcagcaca ctatcggggg
     3661 gagagaagac aaaagtccta ctggcacttt tatttgtgga tgacactcat ttcccgttaa
     3721 tcgatgaacc aacgaatcat ttggatatct ctggtagaaa acaagtagcg gcttatttga
     3781 aaaagaaaaa acaaggcttc atcgtggtca gccatgaccg gggatttatc gatgaagtag
     3841 tggaccatgt tttagcaatc gaaaaaagtc aactggaact ttatcaaggg aatttctcta
     3901 tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga
     3961 aaaaagaagt cagtaggcta aagaaaacag cagctgaaaa agccgaatgg tctcgttccc
     4021 gagaaggaga taaaacaaag aaacaagtcg gattcatcga tactgaatct agacgagtga
     4081 ataaaggagc agtgggtgct gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga
     4141 atcggatgga gacccagatc agcgagaaag aaaaactatt aaaagatatc gaatatatcg
     4201 attctttgac gatgaatagc caagcgtctc accataagcg acttttaagc gtagaagatc
     4261 ttcaattagg gtatgaaaat ctgttattcg agccaattca ttttacaatc gagcctcatc
     4321 agcgggtggc gatttcaggt cctaacggtg caggaaagtc atccattatc cattatcttc
     4381 tgggggcatt caacggcaag gttataggag aaaaatacca gccaaaacat ctgagcatta
     4441 gttatgcaag ccaaaattat gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa
     4501 accaagtaga ctaccaagca tttttgaaca acctccgaaa gcttgggatg gaaagagatg
     4561 tttttcataa caagatcgag cagatgagta tgggccaacg gaaaaaagtg gaattggcta
     4621 aatctttatc acagccagct gaactatata catgggatga accattgaat tatttggatg
     4681 tcttcaatca agaacaatta gaacaactga tcttgaacgt gaaacctgcc atgttactag
     4741 tggaacatga tcaaaccttc ctggataaag tatctactga gattatttct cttgagagaa
     4801 tctaaccctt gtaatctcaa gaacaacaag gtatggttaa ggggaacttt gccaacgaag
     4861 gagagaatca catgctttat gtaacgagac acggagaaac aacttggaac gcacaaggat
     4921 tagtttgcgg acgggcagat gtgccgctga ctgaaaaagg acagatgcag gcacagaaac
     4981 tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa aatcatccat tcccctcttc
     5041 aacgagcaag agatacagca caagcagttg ctgatcgtct cagcttgccg ctgaccgttg
     5101 atgaacgatt ggtagaaatg gattttgggg attatgatgg actgccaagc aaagatgaga
     5161 actttcaaaa agcacgttta gcgttcgctg tacgattccc aaatggggag tcagtcttag
     5221 atgtgtatgc acggatcgtt cctttattga aagaatgcat cgaagacgaa gaaaatgtct
     5281 atctgttagt ttgccataat gccttgataa gagtgatcaa tgcttatttt catccgatgc
     5341 ctaatgaggg gttctttaca ttcatggtag ataatacaga attggtttct tatgagtaat
     5401 tatgtttttg tgttatttaa ataaaatatt tcaaggcact gcttttgctt agcagtgcct
     5461 tttttaggtt atgacaattc ttttgataat ataaactcaa cctttagaaa tgaaatttaa
     5521 ttattttaat aaaagtattg aaattttgtt tctttagata tatactctag tcatagaaat
     5581 gaagacggat acagaaaaag agggataata tgtttggctt ttcaatattt atgaacgatt
     5641 ctttgtcaga agagaagaaa cagtatatcc gccaaatggc atccaatggc tttgtcggta
     5701 ttttcacttc catgcatata ccagaagatg atgtttcggc atatcaaaag cgattgatcg
     5761 aactaggaag cgaagcgaaa aagaataaac tagaattgat ggtcgatatt tcaggagagg
     5821 cattggaacg agcgggtttt tcattagaag atctaacgcc tttgaaagca attggtgtaa
     5881 caggtttaag aatggattat catatttcca atcagcagat tgccgactgg tcacatcaat
     5941 tgaaaatcag tttgaatgct agcacgatca cgcctaaaga tatcgatgaa ttgaaagaag
     6001 cagaagctga tttctcacag attgaagctt ggcataatta ttatccacgt cccgaaacag
     6061 ggctggacaa agaatggtat cagaaaaaaa atcaatggct caaaattcaa ggtctccttg
     6121 tccaaggttt tgtaccagga gatacagagt tgagaggccc actatatcaa ggattgccaa
     6181 ctttggaaga acatcgaggg atccatcctc ttgcaggcgc tctagatcta ttagcaagta
     6241 acacggatat tgtttacatc ggagatgcag gtctgtcaga aaacgttcag gagcaatttg
     6301 cttctttcca aaaagaacag acagtcttgc tccatacgga acctgtagat gaagaatttt
     6361 acgaatatat attagggaag catacgaatc ggcaagacga tgcaagagat gtcatacgaa
     6421 gcgtagatgc acgttttcga gagattccgc ctatcccagc aagaaataca gctacgcgta
     6481 tgaaagggtc tattacttta gataacgaaa agtatttgcg ctatatgggt gagatccaat
     6541 taaccaaata tgatttgcca gcggatgaaa aagtgaatgt agtagctaaa gttatcaaag
     6601 aagagctacc attgatcaac caaatcaaag ctggtatgaa ctatcaattt ataagaaaag
     6661 aaggaagata aaaatgagca aaatcaattt agagaaccta acaacggaac gcagaaacca
     6721 agaaactttt ggattagatg agatgagtat ttctcaagcc gttcaactaa tgaacaaaga
     6781 agatcagaaa gtggccgctg cggttgaaaa agaactgaca agtatcgaac cagtgattgc
     6841 taacacgatt gagtccttta aaaaaggtgg acgtctgatt tatatgggag ccggaacaag
     6901 tggaagacta ggtgtgttag atgctgctga atgcgtaccg acattcggtg tagaaccgga
     6961 aatggtcata ggtctaattg ctggtggaga agaagcaatg actgtagctg tcgaaggggc
     7021 cgaagattct tatgagctag ggcaagaaga tctaatcaag ctgaatttga caaaaaatga
     7081 tatagttatt ggtatcgcag ctagcggtcg tacaccatat gtgatcggtg gattagatta
     7141 cgcaaaatcg atcggtgctg tcacaggaac gatttcttgc aataaacaag ccgaaatcag
     7201 taaacacgcg gatttcccaa tcgaagtcga tgcaggtcca gaatttttga caggctctac
     7261 tcgattgaag tcaggaacag cacaaaaact tatcttgaat atgatttcta ctatcagtat
     7321 gattggtata ggtaaagtat tcaataactt gatggtcgat gtcaaaccaa caaatgaaaa
     7381 actaatcgaa cgctcaaaac gaatcatcat gcaagcgaca gaagctgatt acgaaacagc
     7441 tgaaaagtac tttgaagaag cagatcaaaa tgttaagctg gctatcgtca tgattttgac
     7501 aaatagtgat aaagaggaag ctcaagcaaa attagctgaa gcaaacggat ttgtaaaagg
     7561 gaccttatga gggggaaaag accatggcaa aaaacgaact gactttggaa gaactggcaa
     7621 aacaaattta taaaggggca ggcggcatgg gcaatgtaga aagcattgtc cactgcatga
     7681 ctcgtgtgcg tatgaagatc atcaatgatg atcttgtaaa tgtctctgaa ttaaaagcga
     7741 ttccaggagt gcttggtgta gtagaagatg aacagcttca agtcatcatc ggacctggga
     7801 aagtgaacaa agtagcacag caaatggttg atttggcagg cgtgaaatta ggagaaaaac
     7861 tttcttctgt aagtacagct acagcaaatt catcagcatc tggaaaagac cgtgtaaacg
     7921 aacgggcaca agaaatgaaa gcggcacaaa aatcgaaaca aaaaccatct aaaatcaaat
     7981 cggtattaaa agacatttct aatatctttg tgccaatgat tcctgcattt gtcggtactg
     8041 ggatcgtggc agggattgcc gcagttttag caaacttagt cacagccgga acacttgatg
     8101 cagcaacatg gcaacaatat atcgatgtga tgaacatctt gaaaaacggg atgtttagct
     8161 acctagttat ctatacaggt atcaatgctg cacaagtttt tggtgcaaca ccaactcttg
     8221 gtggagttat cggggccgtt gtgatgctga cagggatgaa tccggaagca ccactgaaaa
     8281 acttatttac tggtgaagct cttgcagctg gacaaggtgg gatcctaggt gtcatcttcg
     8341 ctgtttggct attgtcaatc gttgagaaaa aactgcataa agtagtacct gatgcggtag
     8401 atatcatcgt tacaccaact ctttcattga ttgccatcgg tctgattgaa attttcttca
     8461 tcatgccttt agcaggtttc atttctgatg gcatggtcgg tgggatcaac tgggtacttg
     8521 gcgttggtgg agcatttgcc ggcttcgttc taggcacatt attcttgccg atggttatgt
     8581 tcggactgca tcaaattttg actccaatcc atgtgcaaat gattgatgaa actggtcgta
     8641 cgctattgct tccaattctt gcgatggctg gtgcaggaca agtgggtgca gcattggcat
     8701 tatggattcg ctgcaaaaaa gataaagaat taacagaaat gatcaaagga gctttaccag
     8761 tcgggatcct aggaatcggc gaaccattga tttacggagt aacattacct ctaggacgcc
     8821 catttattac tgcttgtatc ggtggaggta tcggcggggc agtgatcgga atgatcggaa
     8881 atgtcggggc tatcgctatt ggaccttcag gagccgcttt gatcccattg atctctgacg
     8941 gtaaatggtt aggctacatc cttggattat tggcagctta tgctggtggc ttcgttgcta
     9001 cattcttctt cggtattcct aaagaccgtt tagaaaaaga agaattagcg actgaaacag
     9061 ccacagtaaa tgaagtgcct gaaaaagtag tacctcaaac ttcagctgaa agtgtgacta
     9121 tcctctcatc agttgctgac ggaacagttg tttctttaga taaagcaagt gatccagttt
     9181 ttgcacaaaa gatgatgggt gaaggctact ttgtagaacc agaaaacgga catatctatt
     9241 ctccagttac tggggtagtc agctctgtct tcccaacgaa acacgctatt gggatcacaa
     9301 cgccaaatgg tttagaaatc ttgcttcata tgggaatcaa cacagttgac ttaggcggta
     9361 cgccatttga tgtgaaagta acagaagggc agcaagtgac acctgatacg cttgtagcag
     9421 aagctgactt agaagcgatc aaagcagcag gcaaagaaac atccatgatc gtcttggtaa
     9481 ccaatatgga tcgtgtaaaa aactttgtct tggaaaaaac aggtaaagca aaagcaaaag
     9541 cacctgtgat ggatgtcaaa gcttaatatg atagaaacaa tagaaacgaa agaagaaact
     9601 gaaatctatt tttcagcttc ttctttttta ttatttattg ttttctgcta aaatctgata
     9661 aattcgtgtc acatcagatg gtgttccccg taattcataa tctgccaaga aaggaaagcc
     9721 aaatgtttgt gcatattgtt tggctgttag acaatactga ttattgaagt ttttatttcc
     9781 gctaccgact acaccaagac aaagtttatg attgtcttca tgttccagat attcacgcat
     9841 cgtttcggtc aagatttctg tatcaccgtt atctaaacca tttccgccgt ctaaataagt
     9901 aggaacgaaa gtaaaaaatg gttctgtttc tttcgtaaaa tcactatttt catggatttc
     9961 ttttaaagta acttcaggat tgacaggatc ttctgcatgc attttttcag catattctgc
    10021 tagatgtttg gcaaaggcac gcgtatttcc tgaaatcgat atatataaaa tgttcatgga
    10081 tcatcctcca attctttcct tttagtatag catctttttt aagttttttg gtggtgtaat
    10141 ttggaaaggg actaggcgct ttgcttgtat aatgagaaaa aagccggtag aattatattt
    10201 ctattaaagt ttttattaat taagtaaaaa taaaaaaggt ttgttatact ttttgataaa
    10261 agcggtttta ttttggagga tgcttacatg gatacagagc aacagaaaaa aggaattggt
    10321 ttaattccac ttgctgcctt agtaattggt tcagcgattg gaggtggcgt atttggtatt
    10381 atgacggaca tctccggatc ggctggcggc ccagctattt tcagttgggt actcgtagga
    10441 acagcaatgt tgatgctctc cttgtcgatc aataatatca ataaaa
//
LOCUS       DAYYCQ010000229         7943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.guided.107, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000229 DAYYCQ010000000
VERSION     DAYYCQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.guided.107"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACHAYF_003028"
     CDS             128..1564
                     /locus_tag="ACHAYF_003028"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8580365.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4185
                     /locus_tag="ACHAYF_003029"
     CDS             1753..4185
                     /locus_tag="ACHAYF_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8580366.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            4415..5482
                     /gene="rlmN"
                     /locus_tag="ACHAYF_003030"
     CDS             4415..5482
                     /gene="rlmN"
                     /locus_tag="ACHAYF_003030"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8580367.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5472..5678
                     /locus_tag="ACHAYF_003031"
     CDS             5472..5678
                     /locus_tag="ACHAYF_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580368.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5675..6778)
                     /locus_tag="ACHAYF_003032"
     CDS             complement(5675..6778)
                     /locus_tag="ACHAYF_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8580369.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7330..7878
                     /gene="aac(6')"
                     /locus_tag="ACHAYF_003033"
     CDS             7330..7878
                     /gene="aac(6')"
                     /locus_tag="ACHAYF_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8580370.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2658 a         1357 c         1724 g         2204 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaagttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     2521 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     2581 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     2641 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     2701 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     2761 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     2821 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     2881 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     2941 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     3001 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     3061 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     3121 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     3181 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     3241 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     3301 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     3361 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     3421 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     3481 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     3541 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     3601 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     3661 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     3721 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     3781 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     3841 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     3901 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     3961 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     4021 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     4081 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     4141 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     4201 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     4261 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     4321 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     4381 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     4441 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     4501 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     4561 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     4621 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     4681 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     4741 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     4801 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     4861 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     4921 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     4981 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     5041 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     5101 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     5161 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     5221 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     5281 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     5341 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     5401 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     5461 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     5521 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     5581 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     5641 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     5701 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     5761 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     5821 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     5881 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     5941 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     6001 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     6061 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     6121 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     6181 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     6241 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     6301 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     6361 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     6421 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     6481 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
     6541 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
     6601 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
     6661 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
     6721 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
     6781 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
     6841 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
     6901 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
     6961 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
     7021 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
     7081 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
     7141 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
     7201 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
     7261 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
     7321 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
     7381 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
     7441 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
     7501 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
     7561 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
     7621 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
     7681 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
     7741 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
     7801 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
     7861 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
     7921 gaaggaattt acgaaaatta ctt
//
LOCUS       DAYYCQ010000230         4822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.guided.140, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000230 DAYYCQ010000000
VERSION     DAYYCQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.guided.140"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..1000
                     /locus_tag="ACHAYF_003034"
     CDS             194..1000
                     /locus_tag="ACHAYF_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8580371.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1018..1845
                     /gene="vanW-B"
                     /locus_tag="ACHAYF_003035"
     CDS             1018..1845
                     /gene="vanW-B"
                     /locus_tag="ACHAYF_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFM8580372.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1842..2813
                     /locus_tag="ACHAYF_003036"
     CDS             1842..2813
                     /locus_tag="ACHAYF_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8580373.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2806..3834
                     /gene="vanB"
                     /locus_tag="ACHAYF_003037"
     CDS             2806..3834
                     /gene="vanB"
                     /locus_tag="ACHAYF_003037"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8580374.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3840..4448
                     /locus_tag="ACHAYF_003038"
     CDS             3840..4448
                     /locus_tag="ACHAYF_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8580375.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4514..4687)
                     /locus_tag="ACHAYF_003039"
     CDS             complement(4514..4687)
                     /locus_tag="ACHAYF_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580376.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1361 a         1016 c         1277 g         1168 t
ORIGIN      
        1 gatagaatga ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata
       61 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
      121 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
      181 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
      241 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
      301 ctgcacgctg tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc
      361 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
      421 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
      481 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
      541 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
      601 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
      661 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
      721 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
      781 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
      841 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
      901 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
      961 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     1021 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     1081 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     1141 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     1201 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     1261 ttaaacggct tattgataag accgggggaa accttttctt tctggtggct ggtacgccat
     1321 gcggacaaag atacccccta taaagacggc cttacggtga ccaatggtaa actcaccacc
     1381 atgtcgggcg gcggtatgtg ccagatgagc aatttactat tttggatgtt cctgcatacg
     1441 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     1501 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     1561 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     1621 caggtgttcg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     1681 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     1741 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     1801 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     1861 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     1921 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     1981 ctggcaatca atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg
     2041 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     2101 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     2161 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     2221 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     2281 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     2341 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     2401 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     2461 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     2521 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
     2581 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     2641 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     2701 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     2761 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     2821 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata
     2881 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     2941 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     3001 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     3061 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     3121 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
     3181 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
     3241 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
     3301 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
     3361 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
     3421 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
     3481 attgtcggcg aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa
     3541 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
     3601 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
     3661 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
     3721 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
     3781 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
     3841 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
     3901 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
     3961 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
     4021 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
     4081 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
     4141 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
     4201 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
     4261 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
     4321 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
     4381 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
     4441 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
     4501 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
     4561 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
     4621 cgcccatttt gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg
     4681 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
     4741 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa
     4801 gttgaactat ttaatggact gt
//
LOCUS       DAYYCQ010000231         4736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.guided.143, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000231 DAYYCQ010000000
VERSION     DAYYCQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.guided.143"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..816
                     /locus_tag="ACHAYF_003040"
     CDS             124..816
                     /locus_tag="ACHAYF_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8580377.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1143..1640
                     /gene="dfrG"
                     /locus_tag="ACHAYF_003041"
     CDS             1143..1640
                     /gene="dfrG"
                     /locus_tag="ACHAYF_003041"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8580378.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1712..3664)
                     /locus_tag="ACHAYF_003042"
     CDS             complement(1712..3664)
                     /locus_tag="ACHAYF_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8580379.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3671..3907)
                     /locus_tag="ACHAYF_003043"
     CDS             complement(3671..3907)
                     /locus_tag="ACHAYF_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580380.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4309..4599
                     /locus_tag="ACHAYF_003044"
     CDS             4309..4599
                     /locus_tag="ACHAYF_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580381.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1603 a          786 c          815 g         1532 t
ORIGIN      
        1 gattaaatga gtatttgata gtatttatat taaaattaca tatgtaattt aaaaacgctt
       61 gacattgtgt ttgagatcac ttacttttag catgtaaaag tattggatta aggagctatg
      121 caaatgaaaa agaaatttat tactggtggg attgtcgttg catttctatg tctaattggc
      181 tacacaggca cgcggtacgc aaatgaaatt attttgtggg gtggagagac agatattgat
      241 acaatcaatg agaatctgac acagcttgat catacattga cagataaaga gcaagcgatt
      301 acagatctta ccgctcgact agcacaaaaa gaacagacat tgcaagattc aaaggcagat
      361 gtccaagaat accaaacaaa agtaaatgaa ttagaaaacc aaaaaaatca atcggcagca
      421 gaaaaacaaa acttagaaag tcaattagtc agcaaggatg cacaactaca aagcaaacaa
      481 aatgaaatca atgtcaaact agatgagatc aaccaaaaaa atgatgaaat caatcatctg
      541 aatgcagatt ggacagcaag actacaagaa gcacaagcaa aactttcaga attacaaaat
      601 actgtaaatg gtttgaatca acaaatcgca acactgacac aagaaaaaac aaatgtagaa
      661 actcagttaa cagatacaca aaagaaatta gacgaagcaa ctgctgaaaa ctcaagcttg
      721 aaacaatata ttcaaaagtt agaaaaagca aaagatgaag tggaagatac agctgataaa
      781 tctcagcaaa ttgttgaaga acatttgcgt aaataatctt tcttaacaag taaacctaaa
      841 ttagtaaaga agcaacagta atctatacta agtagttcag ttttggagta caaaatgaca
      901 aaatgtactt caaaatggac tacttttttc attttaactt acataccaaa ttacatacaa
      961 aatgatttat aaaatgcaat tgaaaatgat aattgaatga aaatttgtga ttttgtggta
     1021 ttatgataat ataacttata gaaaaaattg cttattcaac taacgggcag gataatgaga
     1081 aaaggttttt tgatagaaat aagaaattag atggtaaatt agttaaaaag gctgtgttat
     1141 aaatgaaagt ttctttgatt gctgcgatgg ataagaatag agtgattggc aaagagaatg
     1201 acattccttg gaggattccc aaggactggg aatatgttaa aaatactaca aagggacatc
     1261 cgataatatt aggtaggaag aaccttgaat caatcggaag agccttacct gacagaagaa
     1321 atattattct gacgagagat aaggggttta cctttaatgg ttgtgaaatt gttcattcaa
     1381 tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat ttttattttc ggaggagaac
     1441 agatttataa tttgtttttc ccttatgttg agaaaatgta catcacaaaa atacatcatg
     1501 aattcgaagg agatactttt tttccagaag tgaattatga ggaatggaat gaggtatttg
     1561 cccaaaaagg gataaagaat gataaaaatc cgtataacta ctattttcat gtatatgaaa
     1621 gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa gaacagtaaa gataaagcac
     1681 aagagcttgg gattactcca agctcttttt gtcatttgaa ataaattata tctttccaat
     1741 caaattgttt atctgtaatc cctaatcttt gagcaggtgt caaaaccttt ttatccgctg
     1801 atttaaaagg gcgacaaaag ttgtagtaag ttcttagaat tgtaatcgca aattgagcat
     1861 acttcggatt gaagttagcg taaatatagc tcttcttatc accacgagct gttgttaatg
     1921 gtctttcaag agatgatata taacgtctta tctgttgaat aaagctattg gtcgaatggt
     1981 cattaacatt taatagcatc tttgcgattt cgtttggttc cagagcagat aaatccgttc
     2041 tacaatcaac tgaataaaat cctttatcct tagtggctaa tggatgtttg atagggttat
     2101 cggcatattt gcgatatctt tctccatctt tactaatagc ctcttcgtgg aattgatgac
     2161 ttgttagtaa ttcagttaga taatgagatg ccaaagtccg tagaaacttt gttttgaatc
     2221 ctctgatatc tccccaatcg agcagttctg cttttgcttg tccgaattct tttaagcatt
     2281 gttttctgct tttcgtcttg ttaacttggc aaataaaatg atgtgcatcg ctcaacctta
     2341 attctttcgt gaatactcta taaaaggcgt ttctaataga gaagtcatca tcactaatca
     2401 ttcgccattc gtcggcatta accatttgtt taattaacca atagtgagca gttgtagtat
     2461 aaggagcatc aacatttaaa ccatcgacaa atctactcct attagtaatt ttccctaatt
     2521 cgtgaagata agcattccta ttttcgctat cgttagcgga tggttcttgt ggataataag
     2581 accaatctaa cctatcattt ttacgactaa aagtattgag atggtcttct ttgaattttc
     2641 ttgtatcttc atttaattca tccattgaaa tattccaatc ataggcaaca tcagaacgga
     2701 acacatatct tgataagacc tcagcagata caactatata cgtttgcata ttaaggtctt
     2761 caaaccccgt atatttcttt gaaccttgtc ctttcttacg gacgttattt aggtagtagt
     2821 gcatcttgtc cgtattaagc cacatctcgt taaatttctt tgtttgaagg ggttgtgtct
     2881 catatcgttc taaaaactcc aaacaacgcc tatacagcca ctccagctta tgatagtagg
     2941 ttccaactcc aattcctaaa atatcacaag tacgactgac tgaaaccctt ccaacaacca
     3001 tcttagcgaa cattggaaga atggtgttct tttgttggtg ataagtagtg gtttcttctc
     3061 tttttggtag aacattagtg aacttcttac acgccttaca ttgatagcgt tgagatttgc
     3121 ccctactttt cccccttttg taaaattgct tcggctcatt gaatggggtt gattcttctt
     3181 ccgagcagcc ttctttatga aattgataat ctggctcaac atcttggatg ctattgattt
     3241 ctataaggcg tttaatttct tcaacaacag accaattaga taaaggggtt actgtacagt
     3301 tttgcgatac acctctgcca ataggattgt cattgcagta cataccttta tgacctttat
     3361 ctttggaact accagtcatt ttatatcgac ttggtttccc ttttacatct ttgaatttgt
     3421 gctgttcttt cccaaaatta caacaaaacg gattgatgca gtaattatat tgaatgggat
     3481 attcagaacc gttccattta aaagacgttg gcaagaacaa cattttgtta taccttgttg
     3541 caaatcgttt tggagttaat aaatcatatt gtttagccct atcttttaat tcttcgtttg
     3601 aaatcggaat tttaacatct acaatttcat cttccttcgt ggctaatctt ttaagcttgg
     3661 tcattgtcta tcaacccact ctatctttga tacccatttt ttcaacaatc cgcttattac
     3721 ttcgcttgtt ggcaatccat tcaataaact tcttatcgtc taaaatgttc aataagaatt
     3781 catccactgc ttcgctttca gtacgctctg catattcagc gtattgctta actatcaccc
     3841 ttacttgctc tgatatttcc caatccacag acttagcgtt cttatttttt ggctcaagaa
     3901 attgcatagg aaaatgcacc cctttttgct tagtaaattg taaccatttt aacacaccaa
     3961 tttgcattcc tacaataatt atttcacgtt cgcattttta tatacatttt aacttacatt
     4021 ttgtggtgta aattaacaac aaacgttgat ttaacaataa aaaacgagta tcaaattcag
     4081 ttgcaatttg atactcattt aggtttacat tttcactttt tgtactccaa aactgaacta
     4141 cttaaatcta tactgctgcc tctttttttt agtacatgtc cttcatcttc gcaggaagtg
     4201 ccaatatgat tttaagaaag gtaatagaca aaaagtcaaa aagataaaac agagtttctt
     4261 ctttacaaag ggaatgtatg ttcgtataat ggtattgagg tgaaggttat ggagaaaaaa
     4321 tatggaacag tagataagat ccgtactttg caagttagtg ccagaccgtt gatccgtttc
     4381 agtttaaatg gggtcaattg tttgattgct tcccatagtt tgaacttcct agctgaagta
     4441 gacgaaggga tgaagttagt cgttacaggt tattataata atagaaagca atttgttgtg
     4501 agatcctatc atgtgttagg gaaacccaaa attgtggtag aatatgagaa aagtctatac
     4561 ccaagaaaaa aggtccgaca agatgaaaga ataaactaga aaacaagcgg ctgagacaaa
     4621 aatgtcctag ccgcttgttt tggaaagatg gatgcagacg atgggatttg aacccacacg
     4681 agcatacgct cacaagaacc tcaatctata tataactaca tgtatttaag tatttg
//
LOCUS       DAYYCQ010000232         4256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.guided.152, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000232 DAYYCQ010000000
VERSION     DAYYCQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.guided.152"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..187
                     /locus_tag="ACHAYF_003045"
     CDS             <1..187
                     /locus_tag="ACHAYF_003045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580382.1"
                     /translation="KQQFHGWSMSIFSNDSRIGESYDRFGDLVIVISFRVGKDVMQFF
                     CKQIFLLFFSIDNCCFT"
     gene            206..1684
                     /gene="msr(C)"
                     /locus_tag="ACHAYF_003046"
     CDS             206..1684
                     /gene="msr(C)"
                     /locus_tag="ACHAYF_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8580383.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1731..2177
                     /locus_tag="ACHAYF_003047"
     CDS             1731..2177
                     /locus_tag="ACHAYF_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8580384.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2376..2891)
                     /locus_tag="ACHAYF_003048"
     CDS             complement(2376..2891)
                     /locus_tag="ACHAYF_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8580385.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3008..4210
                     /locus_tag="ACHAYF_003049"
     CDS             3008..4210
                     /locus_tag="ACHAYF_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8580386.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1269 a          717 c          926 g         1344 t
ORIGIN      
        1 taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta gaataggtga
       61 aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag gaaaagatgt
      121 gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata attgttgttt
      181 tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa caaatctaca
      241 agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct atcagcaaga
      301 tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca aactaatagc
      361 cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca actacctgcc
      421 tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca gtcatttcca
      481 gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac gaaagatcga
      541 gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc caacaaccca
      601 tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt atggtacgct
      661 ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa tctgggaagt
      721 aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca ctcaaaagga
      781 attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga aaaaacgatt
      841 ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag tgtccagtaa
      901 aaagaaaaag caacaaatca gactggatcg gttgtcttcc tctaaacaaa aagacagtgt
      961 acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa aaataggaga
     1021 aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat ctcttgaact
     1081 ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg ggagaacatt
     1141 actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg tcggtgaaaa
     1201 tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag gaatccttct
     1261 ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga catctgaaga
     1321 atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg tccgtagttt
     1381 gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta cattaagtgg
     1441 gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta atgtgttgct
     1501 gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag agaagctaat
     1561 gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg tcgagcgtac
     1621 ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta cgagaaattt
     1681 ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga ttgcctacaa
     1741 ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc ttggggatga
     1801 gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg attggtctag
     1861 tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg ctggcgaaaa
     1921 aaattgcctt taaactacca gtaaatccag aaagcaaggt ccaaatgatc ttaactattt
     1981 catgcttgat ggttttagga atggtgacat gtatgtcttt attcggtgtg atcatggaac
     2041 aaggaatacc tgatcaatta ttctctgctt atatgaatgc ttggaaaatg aatgtgattg
     2101 ccgctttacc gttgcagttg atcatcgttg gcccgctttc aagaagtgtg ctaaaagcaa
     2161 ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa ggccttggca tgctttttgt
     2221 catagtctct tgaatgcagt tctagaaaaa tagtgatccg aacgataggt tttggcctgt
     2281 cgttcggatc gctttttctt ttccaactgt attgctcttt tattgaagat aatttgctct
     2341 tcaatagata tttctagaaa ggttctgttt atcccttact caaaaaactg ctgcatttgt
     2401 tctcttactt gctgacacca ttggagttcc tgctgacaaa gcataattct gcgctggatc
     2461 acaacataac gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa ttttttttga
     2521 tttttttgca tgattttgtt cagctgttgt tctctagtga atagcagtcc agtagctgtt
     2581 gttttatcga gcgtcgaaaa agcatagatt tttgctagaa attcatcttt ttgcgtagga
     2641 gcgggagttt cctctttgat ccattcctct acaagagcac gtccttcatc agtcaagtgg
     2701 tagatctttt tttgagagtg ctcgtctttg tccaaaacgg tcacataacc ctgctggtct
     2761 aacttggcaa gcgttgtata gatctgactg tgatgggctt cccaaaaaat tgtgatcagc
     2821 tgcttcaatt catacccaga acgtggtttg gataaaagca cgcataataa tatataggct
     2881 aaagtattca taaaaatcct acctctcgtc ttattttctt tattatatca cagtaaagtt
     2941 tccacgtttt acatgtgcat attgacatgt aaaataaatc atgtatgatg ctaatagaga
     3001 gaaagggatg gaaatattgg agaatcaaat agatatacaa gaacaaacaa atgaagaaac
     3061 aaaggggaaa agttcagcgc tagttgtagc agctacctgt atgatcgctt ttatgggagt
     3121 gggtctagta gacccaatct taaaaacgat tgctgcacag ttaaacgcta cgccagctga
     3181 aacgaccctg ttatttacta gttacatgtt agtcactggt gtggtgatgc tatttacagg
     3241 tttcttatct agtcggatcg gtttgaaaaa aacattgatg acaggattgt ttatcatcgt
     3301 tgtattcgct ggtcttggcg gattatctgg gaatatcgga atgctgattg ggttacgtgc
     3361 cggctggggt gtcgggaatg ctttatttgt ctctactgct ttagcaacga tcgtcagtat
     3421 tttagttggt aacacagaaa aagcaatcat gatgtatgaa gcagcacttg gctgcggaat
     3481 ggctgttggc cctctagtcg gtggggtctt gggcagtggt tcatggcgtt atccattttt
     3541 tggggtagca tttttgatgt tttgcggttt tatagcccta gcggtacttt tacctgaaac
     3601 tgagaaacca aagagaaaag taggcctttg ggaaggtgtc aaagcgttaa gcaatcataa
     3661 acttcgttca gttggactaa tcgcattgtt atataatttc ggattcttta ctttacttgc
     3721 gtatgcgcca tttttattga ttggctattc tgaattagaa gtcggttttg tattttttgg
     3781 ctggggtgtg cttctggcta ttgcttcgat tttcgttgca ccggctcttg agcggaagtt
     3841 tacgacttat cggacaattt tagcagcatt tgtcttattc attatttgtt tgctgttatt
     3901 aggagctgga acttctatgc cagtccttgt accgatcagc attgtgttag caggattttt
     3961 ccaaggaatt atcaatacac tgttgacgac gattgctatg gaaataccag gtctgcaacg
     4021 aaacgttgct tcgtccagct atagttttgt ccgctttttc ggtggagctt tagcaccatt
     4081 tattgcaggt gtgattggtg aacatattgg tgaaagctat gcgttttatt ttgcaggtgc
     4141 cattgttctt atcagtgtat gttgtatcat ttatcaccgt aattattttg ctacaaatga
     4201 agaagaataa gaaatcagag gttgtgaaaa agctgtcctg catcaagtca taagaa
//
LOCUS       DAYYCQ010000233         3950 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.guided.156, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000233 DAYYCQ010000000
VERSION     DAYYCQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.guided.156"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..613)
                     /locus_tag="ACHAYF_003050"
                     /pseudo
     CDS             complement(243..613)
                     /locus_tag="ACHAYF_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            758..943
                     /locus_tag="ACHAYF_003051"
     CDS             758..943
                     /locus_tag="ACHAYF_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8580387.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1101..1268
                     /locus_tag="ACHAYF_003052"
     CDS             1101..1268
                     /locus_tag="ACHAYF_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8580388.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1886..2545
                     /gene="vanR-B"
                     /locus_tag="ACHAYF_003053"
     CDS             1886..2545
                     /gene="vanR-B"
                     /locus_tag="ACHAYF_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8580389.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2548..3891
                     /gene="vanS-B"
                     /locus_tag="ACHAYF_003054"
     CDS             2548..3891
                     /gene="vanS-B"
                     /locus_tag="ACHAYF_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8580390.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         1130 a          818 c          981 g         1021 t
ORIGIN      
        1 ccattcaggg gtttggggat ttccccaaca agcaaaacgg acgcggcccg gcagggacgg
       61 ggcaggccgt ttttccggga gtggctacac tccctatgct tgctacgtta ctgtttctcc
      121 actaacaaga cgtttccaaa gccccaaact acccgcaaaa tagccctctg gaaagaaaaa
      181 agtgcaaaaa aatagaagct acgctgtgca gctcccgcta aaagtaaaac agaggatgcc
      241 agttacacat cctccgtttc attcttcttc ggcttttcaa ttagtatggc gctgtcaatc
      301 gcgccctcga tgatcggcag gtattcttcg gggcagagtt tcagcttgtg gctgaccctt
      361 tgccgcagtt cgctttcctc ccgcatgatc tccgggttaa agtagcgttc cacaggaagc
      421 ccgcaggctt taataagctg tatgacaacc ggcagactgg gaatggcccg tcgttttcga
      481 tgttggcaag atagcgccag tcaatattta ttttctctgc cattgctttg cgcgaaacgc
      541 gctttgcttg ccgggccgct ttcacatccg cgccgaaagt ttcaaagccg ggacaatctt
      601 caatattcgc catatcgcac ctcccactta cattgtatat ttcatactct gcccgtggaa
      661 tgttggtata tgcacctttc ttaaagttta tatttcacat tttgtttctt ttattcttcg
      721 agcaaaagaa ttataatgta ccctgtggag gtgtactatg gactacatga cgttgaaaga
      781 ggcaggcgag aaatggggtg tgcccccacg atggataaac tactattgtg ctgcgggacg
      841 catccccggc gctgtaaaga tggcgacgat ctggctcatt cccaaaactg cggaaaagcc
      901 gattgacagg cgtacaaaga aaggaaaggc ggttactgaa tagtataatg aaaccggcgc
      961 attttggata tggttttgaa gagagagaaa gcatggtatt ttgtcacgga aaaactataa
     1021 gatgatagat ggcaggctgc tccagaccga taaaaagtat tctcatctga aacagaaaga
     1081 aaagatttac acatggatgt atgaagaagc gaaactttat catgacagaa atggaaaatg
     1141 cccgaaacaa agacgaggaa gctgctgctg tatcagccgt atatggtaga attgaaaagg
     1201 ccggcatctg gattctttat ggtgaagtct ccagacattg gataactgct ggtctaatgc
     1261 gcctttgagt atggggtgat tgattttaat accggggagg tacggctttt tgaggaaaat
     1321 attctgggat ttacatttgg cgattattac atttcctgta actgcaagga cacggatgat
     1381 gaagatatga ggaatataga cttgctgatt ttctactgtc ttgaacggta ttaggactga
     1441 atctgctgcg cggagtgcgg caagagcgac cggagggagt acacacaggc cctgcctgtg
     1501 tgaaaagcca taccgcaagg tgcattcgca aaaaagaggc cgcgcatttt gctgaggctt
     1561 ggggtgtccc caacaagcaa aacgagaacg ccgcaggcgg gcaagtggtg gatcagaata
     1621 tccgtaaagg atattctgat ccattgcttg ccagtattat cgggatgttt tgacaaccgc
     1681 tgtaatcgaa acatcccaaa cattaagaag gtgttaatta ttattatctt gatttaattc
     1741 gtttaaacca ataatataga tacatacacc atgaaaagaa gcgagtatcc caatgacgga
     1801 tgattaaaat ccgaaaagaa atcgctttga aactacaggg aaactacaga ctgttatgtt
     1861 atcttcttaa aatggaggga tttttatgtc gatacgaatt ctacttgtcg aggatgatga
     1921 tcatatctgt aatacagtaa gggcgtttct ggctgaggca ggatatcagg tggatgcctg
     1981 cacagatgga aatgaggcat acaccaagtt ttacgaaaac acttatcaac tggttattct
     2041 tgatattatg ctgcccggta tgaacgggca tgaacttttg cgtgaatttc gtgcgaaaaa
     2101 tgatactccc attctgatga tgacagccct gtcggatgac gaaaaccaaa tccgggcgtt
     2161 tgatgcagag gcagacgact atgtaacaaa gccattcaag atgcagattt tactaaagcg
     2221 ggtggaagcc ctgctgcggc gcagcggtgc gctggcaaag gaaatccgtg tcggcaggct
     2281 gacacttctg ccggaggatt tcacggtact ttgtgacggt acagagctgc ccctgacacg
     2341 aaaagaattt gaaatccttt tgctgctggt gcagaacaaa ggcagaacct taacgcatga
     2401 aatcattttg tcacgtatat ggggatatga ctttgagggt gatggcagca cagtccacac
     2461 tcatatcaaa aatttgcggg ccaagctgcc ggaaaatatc atcaaaacca ttcgcggtgt
     2521 aggataccga ttggaggaat cataataatg gaaagaaaag ggattttcat taaggttttt
     2581 tcctatacga tcattgtcct gctactgctt gtcggtgtaa cagcaacact gtttgcacag
     2641 caatttgtgt cttatttcag agtgatggaa ttacagcaaa cagtaaaatc ctatcagcca
     2701 ttggtggaac tgattcagaa tagcgatagg cttgatattc aagaggtggc agggctgttt
     2761 cactacaata accaatcctt tgagttttat attgaagata aagagggaag cgtactctat
     2821 gccacaccaa atgccaatac atcaaatagt tttagaccca actttcttta tgtggtacat
     2881 agagatgata atatttcgat tgttgctcaa agcaaggcag gtgtgggatt gctttatcaa
     2941 gggctgacaa ttcggggaat tgttatgatt gcgataatgg ttgtattcag ccttttatgc
     3001 gcgtatatct ttgcgcggca aatgacaacg ccgatcaaag ccttagcgga cagtgcgaat
     3061 aaaatggcaa acctgaaaga tgtaccgccg ccgctggagc gaaaggatga gcttggcgca
     3121 ctggctcatg atatgcattc catgtatgtc aggctgaaag aaaccatcgc aaggctggag
     3181 gatgaaatcg caagggaaca tgagttggag gaaacacagc gatatttctt tgcggcagct
     3241 tctcatgagc taaaaacgcc catcgcggct acaagcgttc tgttggaggg aatgcttgaa
     3301 aatatcggtg actacaaaga tcattctaag tatctgcgcg aatgcatcaa aatgatggat
     3361 aggcagggca aaatcatttc cgaaatactg gagcttgtca gcctgaatga tgggagaatc
     3421 gtacccatag ctgaaccgtt ggacataggg cgcacggttg ccgagttgct gcccgatttt
     3481 caaaccttgg cagaggcaaa caaccagcgg ttcgtcacag atattccagc cgggcaaatt
     3541 gtcctgtccg atccgaggct gctccaaaag gcactatcca atgtcatatt gaatgcagtt
     3601 cagaacacgc cgcagggagg cgaggtacgg atatggagtg agcctggtgc tgaaaaatgc
     3661 cgcctttttg ttttgaacat gggcgttcac attgatgata ctgcgcttcc aaggctgttc
     3721 accccattct atcgcattga tcaggcgcga agcagaaaaa gtgggcgaag cggtttagga
     3781 cttgccatcg tacaaaaaac gctggatgcc atgagcctcc agtatgcgct ggaaaacacc
     3841 tcggatggcg ttttgttctg gctggattta ccgctcacat caacattgta aatatttaaa
     3901 acttaaatga ttttgggctc tttgtcaaat aggactgata gagtgagata
//
LOCUS       DAYYCQ010000234         2771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.guided.171, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000234 DAYYCQ010000000
VERSION     DAYYCQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.guided.171"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHAYF_003055"
     CDS             110..193
                     /locus_tag="ACHAYF_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8580391.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHAYF_003056"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHAYF_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8580392.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACHAYF_003057"
     CDS             complement(1318..2181)
                     /locus_tag="ACHAYF_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8580393.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACHAYF_003058"
     CDS             complement(2183..2455)
                     /locus_tag="ACHAYF_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8580394.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACHAYF_003059"
     CDS             complement(2472..2681)
                     /locus_tag="ACHAYF_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8580395.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          990 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattaa
     2761 tcacttgtga t
//
LOCUS       DAYYCQ010000235          957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5674
            SAMEA115707886-rid22593703.guided.212, whole genome shotgun
            sequence.
ACCESSION   DAYYCQ010000235 DAYYCQ010000000
VERSION     DAYYCQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707886
            Sequence Read Archive: ERR13197612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290173.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:44:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,061
            CDSs (total)                      :: 3,000
            Genes (coding)                    :: 2,922
            CDSs (with protein)               :: 2,922
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 38 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 14 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707886-rid22593703.guided.212"
                     /isolate="VRE5674"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACHAYF_003060"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACHAYF_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACHAYF_003061"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACHAYF_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          329 a          171 c          125 g          332 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggtt
//
